P15080: Flow Culture System

Multi-Disciplinary Senior DesignProblem Definition Review

September 11, 2014Collin BurkhartKatelyn Busse

Michelle GarofaloRobert Repetti

Morgan StoesselSarah Tran

Agenda● Project Background● Project Statement● Stakeholders● Use Scenarios ● Customer Needs and

Constraints● Engineering

Requirements

● House of Quality● Benchmarking ● Top Risks● Deliverables ● Project Plan● Questions

Project Background

● Cell culture○ What is it?

● Motivation for this project

Problem Statement

● Current Status○ 2D - Static○ 3D - Static

● Current Limitations○ Macro vs. Microfluidics○ Cell Environment in

vivo● The Main Objective

Stakeholders ● Dr. Jennifer Bailey● Biomedical engineering students taking

Advanced Cell Culture Class● Dr. Dan Phillips● Biomedical Engineering Department● Rochester Institute of Technology● MSD Team (and potential future MSD

Teams)

Communication Plan● Follow up meeting with Dr. Bailey every two

weeks● E-mail customer with questions and

concerns at any time● Discuss budget with Dr. Phillips● Reach out to Dr. Man and Dr. Gaborski for

endothelial expertise

Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells

RIT

Instructor

Student(s)

Advanced Cell Culture class

offered

Retrieves flow culture system from

storage

Inspects components for damage

Has students perform

experiment

Assembles components

of system

Sterilizes empty system in autoclave

Transport system to biosafety

hood

Replaces damaged

components

Damaged?

Yes

No

Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells

RIT

Instructor

Student(s)

Add sterile media to

media reservoir

Seed growth plate

Add seeded growth plate

to growth chamber

Transfer to incubator

Power pump and sensory equipment

Activate pump to

initiate media flow

Ensure flow is continuous and free of

bubbles

Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells

RIT

Instructor

Student(s)

Adjust pump flow rate for desired shear

stress

Adjust incubator for

desired pH value

Adjust incubator

temperature as desired

Allow cells to grow

Cycle through new media every 3-4

days

Disconnect growth

chamber to view cells

Reconnect growth

chamber after viewing

Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells

RIT

Instructor

Student(s)

End experiment

after two weeks

Deactivate pump

Cut power to pump and

sensory equipment

Transfer system to biosafety

hood

Disconnect growth

chamber

Drain remaining

media from system

Observe cells in growth

chamber on microscopes

Use Scenario Flow Culture Lab Experiment to Grow Endothelial Cells

RIT

Instructor

Student(s)

Advanced Cell Culture class

ends

Students have completed experiment

Drain growth chamber

Autoclave all equipment

Return equipment to

instructor

Returns equipment to

storage

Process repeats for additional

experiments

Customer Requirements

Importance: 1=Essential, 2=Customer Want, 3=Preference

Essential Customer Requirements

Sterility, Temperature, pH

Constraints ● It is assumed that the flow culture system will be using

only animal endothelial cell lines● This system may need to sit in an incubator to control

temperature, media pH, and relative humidity● Chamber that holds cells needs to fit on defined

microscope stages● System must be sterilized by autoclave● Budget of ~$1550

Engineering Requirements

Importance: 1=Essential, 2=Customer Want, 3=Preference

Essential Engineering Requirements

Essential Engineering RequirementsDescription and Tests

House of Quality

● Every customer requirement has been satisfied by at least one engineering requirement.

● Diagram can be found at the following location on EDGE:http://edge.rit.edu/edge/P15080/public/Problem%20Definition%20Documents/Additional%20DocumentsP15080_House_of_Quality document

Benchmarking● Perform cell culture in BME Lab with Dr.

Bailey● Research and contact representatives of

current companies that market cell culture flow devices

● Confer with Dr. Gaborski and Dr. Man

Top RisksBudget: ● Materials too expensiveSchedule: ● Team member delays● Unexpected labor hoursTechnical:● Unable to keep materials sterile● Material malfunction● Pump inadequacies● Lack of knowledge in electrical engineering spectrum for sensor

configuration

Project Plan

Deliverables for Next Cycle

● Functional Decomposition 9/18● Concept Generation 9/23● Morph Table 9/23● Function/Engineering Analysis 9/23● Feasibility Analysis 9/25● System Design Review 9/30 or 10/2

Questions,Comments,

orConcerns?

Thank you!