ORIGINAL GEL IMAGES for Figure 410.1038/s42255-020-025… · ORIGINAL GEL IMAGES for Figure 4...
Transcript of ORIGINAL GEL IMAGES for Figure 410.1038/s42255-020-025… · ORIGINAL GEL IMAGES for Figure 4...
1
ORIGINAL GEL IMAGES for Figure 4
Figure 4d – Actin (42 kDa)
Figure 4d – ATF4 and cleaved ATF6 (49 kDa and 55 kDa)
Figure 4d – Caspase-3 (19/17 kDa)
Figure 4d – NRF2 / TXNIP (61 kDa and 50 kDa)
Control Rnls- - - + + + - - - + + + TG
75Kd
37Kd
Scanned by C-DiGit Blot Scanner, LI-COR
Scanned by C-DiGit Blot Scanner, LI-COR
-15 Kd
-20 KdControl Rnls
- - - + + + - - - + + + TG
Anti-cleaved Caspase 3
Scanned by C-DiGit Blot Scanner, LI-COR
Control Rnls- - - + + + - - - + + + TG
Anti-NRF2 (61Kd)
Anti-Txnip (~50 Kd)
2
Figure 4d – phosphorylated eIF2alpha (38 kDa)
Figure 4d – phosphorylated IRE1alpha (110 kDa)
Figure 4d – phosphorylated PERK (170 kDa)
Figure 4d – total eIF2alpha (38 kDa)
Figure 4d – total IRE1alpha (130 kDa)
Control Rnls
- - - + + + - - - + + + TG
Scanned by C-DiGit Blot Scanner, LI-COR Anti-phospho-eIF2a
37Kd-
Scanned by C-DiGit Blot Scanner, LI-COR
Control Rnls- - - + + + - - - + + + TG
Scanned by C-DiGit Blot Scanner, LI-COR
37Kd-
50Kd- Control Rnls- - - + + + - - - + + + TG
Anti-Total eIF2a
Scanned by C-DiGit Blot Scanner, LI-COR
Control Rnls- - - + + + - - - + + + TG
Scanned by C-DiGit Blot Scanner, LI-CORAnti-Total IRE1a
3
Figure 4d – total PERK (150 kDa)
Figure 4e (Xbp1u:473bp; Xbp1s:447bp)
Control Rnls- - - + + + - - - + + + TG
150Kd-
250Kd-
Scanned by C-DiGit Blot Scanner, LI-COR
Anti-Total PERK 150 Kd
500 bp
400 bp
300 bp
200 bp
650 bp
non-speci!c Xbp1u 473 bp
Xbp1s 447 bp
Control Rnls- - - + + + - - - + + + TG
mut