Int J Clin Exp Med 2015;8(11):20279-20286www.ijcem.com /ISSN:1940-5901/IJCEM0015047

Original ArticleThe protective effects of total phenols in magnolia officinalix rehd. et wils on gastrointestinal tract dysmotility is mainly based on its influence on interstitial cells of cajal

Hui Tian1, Dazhi Huang2, Tao Li3, Lihua Huang2, Xingguang Zheng2, Danxia Tang2, Lu Zhang2, Jian Wang2

1School of Life Science and Technology, Mianyang Normal University, Mianyang, China; 2School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China; 3School of Science, China Pharmaceutical University, Nanjing, China

Received August 25, 2015; Accepted October 25, 2015; Epub November 15, 2015; Published November 30, 2015

Abstract: Magnolia officinalix Rehd. et Wils is a kind of herb which is widely used for gastrointestinal tract mobility disorder in Asian countries. In this study, we investigated whether the total phenols of Magnolia officinalix Rehd. et Wils (TPM) treatment improves gastrointestinal tract dysmobility induced by intraperitoneal injection of atropine (5 mg/kg) in rats. Rats were randomly grouped into three units: TPM-pretreated/atropine-treated group, atropine-treated group and control group. TPM were administrated for 7 days. Gastric residual rate and intestinal transit were measured 20 min after atropine injected, and gastrointestinal hormones (including: gastrin (GAS), motilin (MTL), somatostatin (SS) and p substance (PS) levels in serum were also measured by ELISA kits. The number and distribution of interstitial cells of Cajal (ICCs) in stomach were detected by immunohistochemistry analysis, while c-kit and stem cell factor (SCF) expressions in stomach were also measured by western blotting. We found that TPM pretreatment significantly improved atropine-induced gastric residual rate increase, while had no significantly effects on intestinal transit; it also significantly normalized GAS, MTL and PS serum levels. Atropine-induced ICCs numbers decreased in both sinuses ventriculi and body of stomach, which is improved by TPM pretreatment. West-ern blotting results showed the expressions of c-kit and SCF were down-regulated after atropine injection, which can be reversed with TPM pretreatment. These results above indicates that TPM treatment can significantly protected atropine-induced gastric dysmoblility, which may owed to its regulation on c-kit/SCF signing pathway.

Keywords: Magnolia officinalix Rehd. et Wils, gastrointestinal tract, mobility disorder, interstitial cells of Cajal (ICCs)

Introduction

Magnolia officinalix Rehd. et Wils is a herb with long tradition and a large variety of uses on treatment of dyspepsia, abdominal distention and constipation among Asian countries. Modern natural product chemical and pharma-cological researches showed that the extracts (alcohol or aqueous) obtained from this plant’s dried bark had the activities on antioxidant and antiviral [1]. Our previous study indicated that the total phenols of Magnolia officinalix Rehd. et Wils (TPM) , which is mainly including magno-lol and honokiol, showed the significant protec-tive effects on dyspepsia and gastrointestinal (GI) tract motility disorders. However, the cellu-

lar and molecular mechanisms underlying the effects of TPM on GI tract motility have not been explored to date.

Gastric motility plays a very important role in digestion, which is precisely regulated by neuro-modulators and hormones. The neuroregula-tion of gastric motility includes parasympathet-ic and sympathetic nerve regulation, and the enteric nervous system (ENS) which is distrib-uted almost every area from stomach to anus. ENS’s vital functions have drawn researchers’ attention in recent years. Interstitial cells of Cajal (ICCs) is a kind of specialized cells in GI tract, which function as a pacemaker and gen-erate spontaneous electrical activity (slow

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wave), timing the phase contractions of the tunica muscularis in GI tract [2]. Dysfunctions of ICCs have been associated with several GI mobility disorders, including irritable bowel syn-drome [3], diabetic gastroenteropathy [4], and intestinal pseudoobstruction [5]. ICCs express c-kit, a tyrosine kinase receptor, which plays a critical role in the control of ICCs growth, dif-ferentiation, and apoptosis, and the mainte-nance of rhythmic activities in the GI tract [6]. The natural ligand for c-kit has been identified as stem cell factor (SCF), which activates the c-kit receptor, inducing multiple downstream signaling pathways [7]. Disrupting SCF/c-kit function impairs the development of functional

Materials and methods

Plant materials

The bark of Magnolia officinalix Rehd. et Wils was purchased from Hehuachi herbal medicine market (Chengdu, China), and identified by Prof. Xianming Lu, a veteran botanist from Chengdu University of Traditional Chinese Medicine. The extract of total phenols of Magnolia officinalix Rehd. et Wils (TPM) was yielded by 90% etha-nol heating reflux of the barks for 60 min, then vacuum dried. The high performance liquid chromatography (HPLC) (Agilent 1200) of the extracts was performed with a monitoring

Figure 1. The HPLC profile of total phenols of Magnolia officinalix Rehd. et Wils (TPM). A. HPLC chromatogram for the standard substances of magnolol and honokiol; B. HPLC chromatogram for total phenols of Magnolia officinalix Rehd. et Wils (TPM).

ICCs, and results in a defect in the ENS, which finally lead to the dysfunction of GI tract. In the other hand, gastrointestinal hormones also play an important role on regulation GI tract’s functions. For example, gastrin (GAS) promotes gastric acid secretion and peristole [8], motilin (MTL) regulates gastric emptying and the migrating motor complex of small intestine [9]. Thus, gastrointestinal hormones levels disorder can also cause dysfunction of GI tract.

In this study, we investigat-ed the mechanism by which TPM protects against atro-pine-induced gastric dys-motility in rats. We found that TPM effectively pre-vents atropine-induced ch- anges in ICCs distribution and gastrointestinal hor-mones levels, reverses at- ropine-induced inhibition of the SCF/c-kit signaling pathway in stomach, and improves atropine-induced gastric dysmotility. Our results indicated that TPM prevents atropine-induced gastric dysmotility by regu-lating ICCs’ function.

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wavelength of 203 nm, Inertsil R ODS-2 C18 (250 mm×4.6 mm, 5 μm), a column tempera-ture of 35 °C, a mobile phase of acetonitrile/water (11:9, v/v), and a flow rate of 1.0 mL/min. The chromatographic profiles of the extract are shown in Figure 1. Magnolol and honokiol were regarded as markers (Sigma, USA).

Animals and experimental protocols

The study was conducted in accordance to the Laboratory Animal Care Committee at Chengdu University of Traditional Chinese Medicine. 24 adult male Sprague-Dawley rats (10-week old and weighing 250-300 g, supplied by Sichuan Chengdu Dashuo Biotechnology Ltd) were housed at room temperature (25°C) with 60% humidity and a 12-h light/dark cycle. Rats were fed standard rat chow and water ad libitum.

The rats were assigned to three groups (8 rats for each group): TPM- pretreated/atropine-treated group, atropine-treated group, and con-trol group. Rats in the TPM-pretreated/atro-pine-treated group received a gavage of TPM (14.00×10-2 g/kg body weight). Rats in the atropine-treated or control group were received a gavage of normal saline (placebo control), respectively. Drug pretreatment lasts for 7 days. At end of experiment, rats were deprived of food (free to take water) for 18 h, and gastro-intestinal dysmotility was induced by an in- traperitoneal injection of atropine sulphate [10] (5 mg/kg body weight, Sigma Aldrich, USA) 60min after the last time TPM administration; Meanwhile, animals in the control group were injected with the same volume.

trifuged for obtaining serum. Then, stomach was removed, washed with cold PBS, dried with filter paper immediately after cardia and pylo-rus were ligated, and then weighted with/with-out removing gastric contents. The gastric emp-tying was evaluated by gastric residual rate, which was calculated as: weight (with gastric contents removed)/weight (without gastric con-tents removed) ×100%. Meanwhile, the small intestine was also removed. The distance of carbon powder transmission as measured from the gastric pylorus to the distal small intestine. Intestinal transit was calculated as: (distance of carbon powder transmission/total length of small intestine) ×100%.

Measurement of gastrointestinal hormones in serum

Gastrin (GAS), motilin (MTL), somatostatin (SS) and p substance (PS) levels in serum were measured by using enzyme-linked immunosor-bent assay (ELISA) kits (Abcam, USA).

Immunohistochemistry analysis

The sinuses ventriculi and 1/3 body of stomach (0.5 cm×0.5 cm) was removed immediately after measurement of gastric residual rate. Affiliated tissues (such as adipose) were removed, and the tissues were fixed with 4% (W/V) paraformaldehyde (pH 7.4) for 8 h. After fixation, the tissues were dehydrated by gradi-ent flushing with low concentration to high con-centration of ethanol under 4°C for 30 min. Then, the tissue was sectioned with paraffin embedding after xylene transparent. The immu-

Figure 2. The protective effects of TPM on atropine-induced gastric and intes-tinal dysmobility in rats. Gastric residual rate (A) and intestinal transit (B) were measured in control (Ctrl), atropine-treated (Atropine) and TPM-pretreated/atropine-treated (TPM+atropine) rats via the method described above. Data are shown as mean ± SD (n = 8). *: P < 0.05, **: P < 0.01.

Measurement of gastroin-testinal (GI) tract transit

Method for GI tract tran- sit measurement was im- proved on the basis of method described previ-ously [11]. 20 min after atropine or saline injection, a 12% carbon powder sus-pension (0.2 mL/10 g body weight) was administered to the rats intragastrically. The animals were then euthanized and dissected after another 20 min. Blood samples were collected via aorta abdominalis and cen-

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nostaining was performed as previously described [12]. Briefly, tissues were incubated in goat serum (99%) at room temperature for 20 min to reduce nonspecific antibody binding, and then incubated with primary antibodies against c-kit (rabbit anti-rat, 1:200 dilution, Abcam, USA) at 25°C for 60 min, followed by incubation at 4°C for 24 h. After primary anti-body was removed by PBS washing, immunore-activity was detected by incubation in HRP-coupled secondary antibody (goat anti-rabbit IgG H&L, 1:100 dilution, Abcam, USA) at room temperature for 60 min. Nuclei were counter-stained by using hematoxylin (Sigma, USA). Section was examined by using BA200 digital microscopic camera system (Motic, China), and optical density was analyzed by using Image-Pro Plus 6.0 system (Media Cybernetics, US).

Western blotting

Western blot was performed to check the SCF/c-kit signaling pathway. 1/3 upper parts of stomach tissues (gastric antrum and pace making area) were homogenized in RIPA lysis buffer, then sonicated and incubated on ice for 15 min. Protein was extracted by a total protein

between groups were tested for statistical sig-nificance by using one-way analysis of variance (ANOVA) followed by least significant difference (LSD) method for multiple comparison tests. P values < 0.05 were considered statistically significant.

Results

TPM improves atropine-induced dysmotility of gastrointestinal tract

To examine the protective effect of TPM on GI tract dysmotility, we investigated the gastric emptying and intestinal transit of rats in con-trol, atropine-treated, and TPM-pretreated/atropine-treated groups. Data were shown that, gastric residual rate was significantly higher in atropine-treated group than in control group (P < 0.01), while intestinal transit was significantly higher in atropine-treated group than in control group (P < 0.01), which indicated that atropine can reduced gastric emptying and small intesti-nal motility as expected. TPM treatment signifi-cantly reduced gastric residual rate (P < 0.01), while had no significant influences on intestinal transit in atropine-treated rats compared with

Figure 3. The regulation of TPM on gastrointestinal hormones which were inter-rupted by atropine-treatment in rats. The serum levels of gastrointestinal hor-mones, which including gastrin (GAS) (A), motilin (MTL) (B), somatostatin (SS) (C) and p substance (PS) (D), were measured in control (Ctrl), atropine-treated (Atropine) and TPM-pretreated/atropine-treated (TPM + atropine) rats via ELISA method. Data are shown as mean ± SD (n = 8). *: P < 0.05, **: P < 0.01.

extraction kit (Chemgene, China). The proteins were separated by SDS-PAGE gel and electrophoretically transferred onto nitrocellu-lose membrane. After being blocked, the membranes were then incubated with primary antibodies again- st c-kit (dilution 1:1000, Abcam, USA) or SCF (dilu-tion 1:1000, Abcam, USA) at 4°C, overnight. β-actin was used as an internal control. Bands were visual-ized using HRP-coupled secondary antibody (dilu-tion 1:2,000, Cell Signaling Technology, USA). Detec- tion was performed by densitometry using the enhanced chemilumines-cence detection system (Bio-RAD, USA).

Statistical analysis

Results are expresses as mean ± standard devia- tion (SD). Data differences

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the placebo-treated group (P < 0.05), which indicated that TPM can improve gastric empty-ing reducing induced by atropine. (Data are pre-sented in Figure 2).

TPM normalizes the gastrointestinal hormones levels which were disordered by atropine treat-ment

As the important role of gastrointestinal hor-mones in regulation of GI tract, we measured the serum levels of gastrointestinal hormones,

which including, gastrin (GAS), motilin (MTL), somatostatin (SS) and p substance (PS) in each group’s rats. Data were shown that, GAS, MTL, and PS levels were significantly down-regulated in atropine-treated group compared with con-trol group (P < 0.01), while there was no signifi-cant changes in SS levels. TPM treatment sig-nificantly reversed atropine-induced GAS, MTL, and PS levels down-regulation (P < 0.01), while had no significant effects on SS level. Results indicated that atropine can induce gastrointes-

Figure 4. The protective effects of TPM on atropine-induced ICCs damage in rats’ stomach. The distribution and amount of ICCs in sinuses ventriculi and body of stomach were evaluated by immunohistochemistry analysis in control (Ctrl), atropine-treated (Atropine) and TPM-pretreated/atropine-treated (TPM + atropine) rats. Optical density value (A) and representative immunohistochemistry photos (×400 magnification) (B) of each group are presented. Data are shown as mean ± SD (n = 6). *: P < 0.05.

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tinal hormones (including GAS, MTL, and PS) levels disorder, which can be improved by TPM treatment. (Data are presented in Figure 3).

TPM treatment attenuates atropine-induced interstitial cells of cajal (ICCs) damage

Since ICCs play an important role in initiating GI mobility, we examined whether ICCs damage takes part in atropine-induced GI tract dys-motility by performing immunohistochemistry analyses using antibodies against c-kit (a mark-er for ICCs in the GI tract) in sinuses ventriculi and body of stomach. Compared with control group, c-kit expressions significantly decreased both in sinuses ventriculi and body of stomach after treated with atropine (P < 0.05). In TPM pre-treat group, c-kit expressions were up-regu-lated both in sinuses ventriculi and body of stomach. These results indicated that atropine can decrease amount of ICCs in rat’s sinuses ventriculi and body of stomach, which impaired production and transmitting of slow wave in stomach, and TPM can protect ICCs from atro-pine-induced damage by increasing the amount

SCF/c-kit signaling pathway. TPM pretreat- ment significantly turn over atropine-induced decrease of c-kit and SCF expressions (P < 0.05), promoting the SCF/c-kit signaling path-way in stomach. (Data are presented in Figure 5).

Discussion

In present study, we have shown that atropine induced inhibition of intestinal transit and gas-tric emptying, as a result of a decrease in the number of ICCs with impairing the c-kit/SCF signing pathway in stomach. In another hand, atropine also induced the decrease of GAS, MTL, and PS levels in serum. TPM treatment effectively prevents atropine-induced gastric dysmotility, restores ICCs numbers, reverses the atropine-induced blockade of the c-kit/SCF signaling pathway, and normalizes GAS, MTL, and PS levels. These results suggest that the ICCs network and the SCF/c-kit signaling path-way play a very important role in the beneficial effects of TPM on atropine-induced GI tract dysmotility.

Figure 5. TPM reversed atropine-induced down-regulation of c-kit and SCF ex-pressions. The c-kit and SCF expressions in control (Ctrl), atropine-treated (At-ropine) and TPM-pretreated/atropine-treated (TPM + atropine) rats’ stomach were measured via western blotting method. The variation folds of c-kit (A) and SCF (B) expressions and protein bands (C) of each group are presented. Data are shown as mean ± SD (n = 6). **: P < 0.01.

of ICCs in sinuses ventriculi and body of stomach. (Data are presented in Figure 4).

TPM treatment up-regu-lates c-kit and SCF expres-sions in atropine-treated rats

As described in the In- troduction section, impair- ed SCF/c-kit signaling path-way can lead to ICCs net-work defection, and finally cause GI tract dysmotility. We investigated whether TPM treatment improved the SCF/c-kit signaling pa- thway which impaired by atropine in stomach. West- ern blotting results showed that c-kit and SCF expres-sions were both significant-ly down-regulated in atro-pine-treated group com- pared with control group (P < 0.05), which suggesting that atropine impairs the

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It is well documented that atropine suppresses GI transit in several animal models [10, 13-15]. Atropine-induced delay of gastric emptying which is caused by its effects on the proximal stomach [16]. Based on previous knowledge, we found that atropine-induced GI tract dys-motility by inhibiting gastric emptying and intes-tinal transit. TPM pretreatment improves atro-pine-induced gastric residual rate, suggesting that TPM has the effects on promoting in gas-tric mobility in rats. Our results provide an experimental evidence for the rationalization of tradition using of Magnolia officinalix Rehd. et Wils for curing GI tract dysfunction.

GAS, distributed almost in whole GI tract, can promote gastric emptying by relaxing pylorus and shrinking sinuses ventriculi [17]. MTL, a kind of brain-gut peptide, play a role of gastric contraction start, and strongly stimulate mechanical movement and electrophysiology activity in GI tract [18]. PS, also a kind of brain-gut peptide, is the strongest hormone on stimu-late smooth muscle excitement in GI tract [19]. While, SS, another kind of brain-gut peptide, can inhibit GI tract mobility and secretion of gastrointestinal hormones [20]. In our research, we found that GAS, MTL and PS serum levels down-regulated by atropine treatment, which can be effectively reversed by TPM. However, there’s no change on SS level. These findings demonstrate that TPM can normalize the gas-trointestinal hormones’ serum levels during GI tract dysfunction. However, the molecular mechanism of TPM’s effects on the gastroin-testinal hormones secretion still remains unclear.

As ICCs play an important role in the regulation of GI tract smooth muscle contraction, damage to ICCs networks is likely responsible for atro-pine-induced GI tract dysmotility. Damage to ICC has been reported to be associated with abnormal GI tract electrical rhythmicity and contraction [21, 22]. Since almost all ICCs express c-kit, distribution of ICCs can be checked via immunohistochemical dye of c-kit [23]. We find that atropine impairs the ICC net-work by decreasing the total number of ICCs, which can impair generation and transmission of slow wave in stomach, and eventually lead to GI tract dysmotility. TPM pretreatment increas-es the number of ICCs, suggesting that the cel-lular and molecular targets of TPM are associ-

ated with ICCs networks. Since c-kit/SCF signaling pathway plays a critical role in the control of ICCs growth and differentiation, and maintenance of rhythmic activities in the GI tract [6]. Disrupting c-kit/SCF function results in a defect in the ICC network, GI mobility, and enteric motor neurotransmission. In this study, we find that atropine impairs the c-kit/SCF sig-naling transduction with reduction of c-kit and SCF expressions. TPM pretreatment effectively improves the expressions of c-kit and SCF in atropine-treated rats and prevents atropine-induced ICCs damage. These data suggest that TPM prevents atropine-induced gastric dys-motility by normalizing ICCs’ function via regula-tion of the SCF/c-kit signaling pathway.

In summary, we studied the effects of TPM pre-treatment on ICCs morphology, c-kit/SCF sign-ing pathway of stomach in a rat model of atro-pine-induced GI tract dysmotility. Our results suggest that the beneficial effects of TPM pre-treatment on atropine-induced GI tract dys-motility is mediated by c-kit/SCF pathway acti-vation in ICCs and normalized the secretion of gastrointestinal hormones.

Acknowledgements

This study was supported by National Na- tural Science Foundation of China (NSFC No.81202933), Sichuan Province Science-technology Support Plan Projects (No. 2013szz025), Sichuan Province Department of Education Science-technology Plan Project (No.14ZA0262), and Mianyang Normal Uni- versity Science-technology Plan Projects (No. QD2014A006).

Disclosure of conflict of interest

None.

Address correspondence to: Dr. Jian Wang, Depart- ment of Pharmacy, Chengdu University of Traditional Chinese Medicine, No. 1166 Liutai Avenue, Cheng- du, Sichuan Province, China. Tel: +86 028 6180 0231; Fax: + 86 028 6180 0234; E-mail: jian-wang08@163.com

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