Operating Instructions MCP Multichannel Pipette ... · Getting to know the MCP 7 1.3 Safety has...

Operating Instructions

METTLER TOLEDOMCP Multichannel Pipette Calibration Systems

Overview of the MCP

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Figure A: Overview 1 Evaluationunit(fordetails,seefigureD) 2 Motorcontrolunit 3 Measuringunit(fordetails,seefigureC)

Figure B: Connections(backoftheinstrument)Evaluation unit: 4 CANsocket(connectiontothemotorcontrolunit) 5 DB-25socket,female(connectiontothemeasuringunit) 6 ConnectionsocketforACadapter

Motor control unit: 7 9-pinD-SUBsocket,female(connectiontothePCviaconverter/hub) 8 CANsocket(connectiontotheevaluationunit) 9 15-pinD-SUBsocket,female(connectiontothemeasuringunit)10 ConnectionsocketforACadapter

Measuring unit: 11 15-pinD-SUBsocket,female(connectiontothemotorcontrolunit)12 DB-25socket,female(connectiontotheevaluationunit)

Figure C: Detailed view of the measuring unit 13 Levelingscrew14 Rackwithcontainers15 Slideforrackcover 16 Rackcover 17 Levelindicator18 Housingwithweighingcellandtransportmechanism 19 Weighingyoke 20 Racksupport

Figure D: Detailed view of the evaluation unit 21 Statusindicatorsforvibrationadapter,weighingprocessadapterandrepeatability(fromlefttoright) 22 Alphanumericdisplay(weightvalue,menu,messages) 23 Calibrationsymbol(appearswhenweighingcellrequirescalibration)24 Operatorkeyswiththefollowingfunctions:

In measuring mode:

Press briefly Press and hold down

«1/10d»,«Cal» Changethedisplayresolution Internalcalibrationoftheweighingcell

«j»,«Menu» Nofunction Callthemenu

«Start»,«f» Startmeasuringcycleorreferencemeasurement Triggerareferencemeasurement

«On/Off»,«k»,«C» SwitchontheMCPCancelaprocessinprogressSetthedisplaytozero

SwitchofftheMCP

In the menu:

Press briefly Press and hold down

«1/10d»,«Cal» Nofunction Nofunction

«j»,«Menu» Selectthenextmenuitem Exitthemenuafterstoringthesettings

«Start»,«f» Changethesetting Nofunction

«On/Off»,«k»,«C» Exitthemenuwithoutstoring Nofunction

Contents

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Contents1 Getting to know the MCP ................................................................................................................................. 6

1.1 Introduction................................................................................................................................................................. 61.2 Whatyoushouldknowabouttheseinstructions............................................................................................................ 61.3 Safetyhaspriority........................................................................................................................................................ 7

2 Standard equipment ........................................................................................................................................ 8

2.1 Contentsofthelargetransportcase............................................................................................................................. 82.2 Contentsofthesmalltransportcase............................................................................................................................. 92.3 Othercomponentssupplied......................................................................................................................................... 9

3 System setup and cabling ............................................................................................................................. 10

3.1 Selectingthelocation................................................................................................................................................ 103.2 Assemblingandlevelingthemeasuringunit............................................................................................................... 103.2.1 PreparingtheMCPfortheverificationofmultichannelpipettes.................................................................................... 103.2.2 PreparingtheMCPforthecalibrationofsingle-channelpipettes.................................................................................. 123.2.3 Levelingthemeasuringunit........................................................................................................................................ 133.3 Systemcabling.......................................................................................................................................................... 14

4 Startup and menu settings............................................................................................................................. 15

4.1 SwitchingtheMCPonandoff..................................................................................................................................... 154.2 Menuoverview........................................................................................................................................................... 154.3 Usingthemenu......................................................................................................................................................... 164.4 Settingthevibrationadapter....................................................................................................................................... 174.5 Settingtheprocessadapter........................................................................................................................................ 174.6 Selectingrepeatability................................................................................................................................................ 174.7 Selectingthenumberofpipettechannels................................................................................................................... 174.8 Activating/deactivatingthesecondmeasurementofcontainer1.................................................................................. 184.9 Definingthefilllimitofthecontainers......................................................................................................................... 184.10 Definingthetimeintervalforreferencemeasurement.................................................................................................. 184.11 Calibratingthecontainers.......................................................................................................................................... 194.12 Resettingtofactorysettings........................................................................................................................................ 19

5 Working with the MCP .................................................................................................................................. 20

5.1 Calibratingtheweighingcell...................................................................................................................................... 205.2 Preparingtheworkstation.......................................................................................................................................... 205.3 Testingmultichannelpipettes..................................................................................................................................... 215.3.1 Fillingthemoisturetrap.............................................................................................................................................. 215.3.2 Referencemeasurement............................................................................................................................................ 215.3.3 Performingthemeasurement..................................................................................................................................... 225.3.4 Emptyingthecontainers............................................................................................................................................. 235.4 Testingsingle-channelpipettes................................................................................................................................... 245.4.1 Fillingthemoisturetrap.............................................................................................................................................. 245.4.2 Performingthemeasurement..................................................................................................................................... 24

6 Status and error messages ............................................................................................................................ 26

Contents

7 Cleaningandservice.................................................................................................................................... 28

8 Technicaldataandaccessories..................................................................................................................... 29

8.1 MCP105data............................................................................................................................................................. 298.2 Ambientconditions.................................................................................................................................................... 298.3 Protectionandstandards............................................................................................................................................ 298.4 Powersupply............................................................................................................................................................. 308.5 RS232Cinterfacedata............................................................................................................................................... 308.6 Dimensionsandweights............................................................................................................................................ 318.7 MCPtechnicalspecifications...................................................................................................................................... 328.8 Accessories............................................................................................................................................................... 328.9 Spareparts................................................................................................................................................................ 33

9 Appendix:MCPandhostcomputer................................................................................................................ 34

9.1 Connectiontothehostcomputer................................................................................................................................ 349.2 PCsoftware............................................................................................................................................................... 359.2.1 PCsoftwarefunctions................................................................................................................................................. 359.2.2 Initializationandconfigurationofthemeasuringsystem.............................................................................................. 369.2.3 Measurementprocedure............................................................................................................................................ 379.2.4 Abortingareferencemeasurement............................................................................................................................. 389.2.5 Abortingapipettemeasurement................................................................................................................................. 409.3 Dataformat............................................................................................................................................................... 429.4 MTSICScommands................................................................................................................................................... 429.4.1 Notesonthecommandformat................................................................................................................................... 429.4.2 StandardSICScommands.......................................................................................................................................... 439.4.3 SpecificSICScommandsfortheMCP.......................................................................................................................... 43

10 Recommendationsforpracticaluse............................................................................................................... 49

10.1 Internalcalibration..................................................................................................................................................... 4910.2 Verificationoftheweighingcellcalibration................................................................................................................. 4910.3 Excessevaporation.................................................................................................................................................... 49

11 Workingwiththeoptionalcalibrationkit........................................................................................................ 0

11.1 Contentsofthecalibrationkit..................................................................................................................................... 5011.2 Installationofthecalibrationkit.................................................................................................................................. 5011.3 Carryingouttheverification........................................................................................................................................ 51

GettingtoknowtheMCP

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1 Getting to know the MCPThissectionprovidesbasicinformationaboutyourMCPmultichannelpipettecalibrationsystem.Pleasereadthissectioncarefully,evenifyoualreadyhaveexperiencewithsimilarsystemsandbesuretofamiliarizeyourselfwiththesafetyinstructions.

1.1 IntroductionThankyouforchoosingtheMCPfromMETTLERTOLEDO.

TheMCPenablesyoutodeterminetheprecisevolumeofpipetteswithoneormorechannels(max.12channels).Todothis,theMCPdeterminestheamountofwaterpipettedforeachchannelandtransfersthisdatatoacomputer,whereitisconvertedintovolumevaluesandprocessedfurther.AspecialapplicationisavailablefromMETTLERTOLEDOforevaluatingthedata,however,theMCPcanalsobeintegratedintoexistingapplications.

TheMCPsupportspipettecalibrationinaccordancewithcommonstandards(suchasISO 8655)whileconsiderablyreducingthetimerequiredforthemeasuringsequence.SeveralMCPcanbecombinedwithoneanothertospeeduptheoperatingsequencefurther.WithanarrayoffiveMCPforexample,thecalibrationofa12-channelpipetterequireslessthanaquarterofanhour(threevolumeswith10measurementseach,accordingtoISO8655).

Thebuilt-inmoisturetrapandanadditionalmeasurementtocompensateforevaporationreducemeasurementerrors,whichcanoccurduetoevaporationofthemediumduringthemeasuringcycle.

TheMCPissuitableformobileuse:Allsystemcomponentsarehousedinastabletransportcase,whichprovidesoptimumprotec-tion.

1.2 What you should know about these instructionsThefollowingconventionsapplytotheoperatinginstructionsasawhole:

Keydesignationsareenclosedindoubleanglebrackets(e.g.«On/Off»or«f»).

Thesesymbolsindicatesafetyandhazardinstructions.Ifthesearenotcompliedwith,injurytotheuser,damagetotheMCPorothertangiblesandmalfunctionscanresult.

Thissymbolindicatesadditionalinformationanddirections,whichifobserved,facilitateyourhandlingoftheMCPandcontributetoproperandeconomicaluseofthesystem.

GettingtoknowtheMCP

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1.3 Safety has priorityOperateandusetheMCPonlyaccordingtothedirectionsintheseoperatinginstructions.

ItisessentialtofollowthestartupinstructionsfortheMCP.

If the system is not used according to the manufacturer’s operating instructions, the instrument protection provided may be impaired (see also section 5.4.4 of EN 60101:01).

TheMCPmayonlybeusedinclosedrooms.Operationinhazardousenvironments isnotpermitted.

OnlyusetheACadaptersuppliedwiththeMCPandensurethatthevoltagevaluesprintedonitcorrespondtothelocallinevoltage.Onlyconnecttheadaptertosocketswithgroundconnection.

All components of the MCP have a rugged construction, but are still precisioninstruments.Treatthemwithappropriatecare.

DonotopenthecomponentsoftheMCP,theydonotcontainanypartsthatcanbemain-tained,repairedorreplacedbytheuser.ShouldyouhaveproblemswithyourMCPontheoddoccasion,pleasecontactyourresponsibleMETTLERTOLEDOdealer.

OnlyuseoptionalequipmentandperipheralssuppliedbyMETTLERTOLEDOwiththeMCP;thesehavebeendesignedtoworkoptimallyinthesystem.

Disposal

InconformancewiththeEuropeanDirective2002/96/EConWasteElectricalandElec-tronicEquipment(WEEE)thisdevicemaynotbedisposedofindomesticwaste.ThisalsoappliestocountriesoutsidetheEU,pertheirspecificrequirements.

Pleasedisposeofthisproductinaccordancewithlocalregulationsatthecollectingpointspecifiedforelectricalandelectronicequipment.

Ifyouhaveanyquestions,pleasecontacttheresponsibleauthorityorthedistributorfromwhichyoupurchasedthisdevice.

Shouldthisdevicebepassedontootherparties(forprivateorprofessionaluse), thecontentofthisregulationmustalsoberelated.

Thankyouforyourcontributiontoenvironmentalprotection.

Standardequipment

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2 Standard equipmentFollowingreceiptoftheMCP,youshouldfirstcheckthescopeofdelivery.Ifthisdoesnotcorrespondtoyourorderorthedeliverynote,pleasecontacttheresponsibleMETTLERTOLEDOdealer.TheillustrationsinthissectionalsoshowyouthecorrectwaytopackallthepartsshouldyoueverneedtotransporttheMCPtoanotherlocation.

2.1 Contents of the large transport caseTheMCPissuppliedinaruggedtransportcase,whichreliablyprotectsthesystemcomponentsduringtransport.

1 Smalltransportcase(forcontentsseesection2.2)

2 Evaluationunit

3 Motorcontrolunit

4 Measuringunit

5 Rackwithmountedrackcoverandslide

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Standardequipment

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6 Racksupport

7 ACadapterforevaluationunit(primary:230 VACor115VAC,50/60Hz;secondary:12VAC)includingwallmountingplate

8 Cablesetconsistingof:

2powercablesfortheACadaptersoftheevaluationunitandthemotorcontrolunit.Ifthescopeofdeliveryincludesasuctionpump(item10),anadditionalpowercableissupplied.

1balancecable(connectioncableforevaluationunit/measuringunit60 cmlong,25-pin,m/m)

1CANcable(connectioncableforevaluationunit/motorcontrolunit30cmlong,m/m)

1controlcable(connectioncableformotorcontrolunit/measuringunit60cmlong,15-pin,m/m)

1USB/serialconverter

9 ACadapterformotorcontrolunit(primary:100-240 VAC,50/60Hz,0.8A;secondary:12VDC,2.25A).Ifthescopeofdeliveryincludesasuctionpump(item10),anadditionalACadapterforthepumpissupplied.

10 Single-channelsuctionpumpforemptyingthecontainers(Note:ForsystemsthatincludeseveralMCP,onlyonesingle-chan-nelsuctionpumpissupplied).Anoptional4-channelsuctionpumpisavailabletoincreaseefficiencywhenemptyingthecontainers,seesection8.8.

2.2 Contents of the small transport caseThesmalltransportcaseishousedinthelargecaseandcontainsthefollowingparts:

11 Containers(13pieces)

12 Waterreservoirwithcoverandholder

13 Weighingyoke(Caution:Donotsqueezetheyokewhenremovingfromthecase!)

14 Single-channelkit (parts insidethe dashed red frame). Afterinstallation of this kit the MCPis ready for the calibration ofsingle-channelpipettes.

15 Smallparts(rubberconeforremovingtheglasstubesofcontainers,tubesforthesuc-tionpump,etc.)

2.3 Other components suppliedAUSB/serial converterincludingdriversoftwareandinstructionsisalsosuppliedwitheachMCP.ThiscanbeusedtoconnecttheMCPtoaPCusingaUSBinterface.ForsystemsthatincludeseveralMCP,aUSB hubisalsosupplied(includingapowersupplywithamainsplugadaptorspecifictothecountry).EachMCPinthesystemisconnectedtothehubviatheUSB/serialconverter,whichinturnisconnectedtothePC(Note:Dependingonthecurrenttechnologyusedandavailability,ahubwithanintegratedUSB/serialconvertermaybesupplied).Alternatively,eachMCPcanbeconnecteddirectlytoanRS232CinterfaceonthePC.

Dependingonthescopeofyourorder,thedeliverymayincludeotheroptionalitems,whicharenotlistedabove(e.g.,PCsoftware,calibrationkitforthecontrolofinspection,measuringandtestequipment,etc.).

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Systemsetupandcabling

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3 System setup and cablingThissectionprovidesinformationaboutsettingupandselectingthelocationfortheMCP.

3.1 Selecting the locationPleaseobservethefollowingnoteswhenselectingasuitablelocation:

– Dry,closedroom

– Stable,vibration-freeandhorizontalsurface

– Nostrongfluctuationsintheambienttemperatureoratmospherichumidity.For good results the ambient humidity should be in the range of 50 - 60 % rh.Nodrafts(windows,doors,airconditioningunits,extractionhoods,etc.)andnodirectsunlight.

– Operationinhazardousenvironmentsisnotpermitted

3.2 Assembling and leveling the measuring unitTheevaluationunitandmotorcontrolunitaresuppliedreadyforcablinganddonotneedtobemounted.Themeasuringunitconsistsofdifferentcomponentswhichmustbeassembled.Therearetwoassemblyprocedures,oneforthecalibrationofmultichannelpipettesandoneforsingle-channelpipettes.Thenexttwosectionscoverbothproceduresseparately.

3.2.1 Preparing the MCP for the verification of multichannel pipettes

Placethemeasuringunitattheselectedlocation.

Inserttherack supportinthefrontposition(towardsthefrontpanel)inthemeasuringunit(A).Thepinontheracksupportmustbeontheright-handside(B).Note:Iftheracksupportisnotinstalledcorrectly,itwillnotbelevelandtheMCPwillnotworkproperly.

Duringtransportation,theweighing yoke support of the mea-suring cellmaybecomemisaligned,i.e.itisnotlocatedinthecenteroftheopeninginthemeasuringunit.Inthiscase,useasmall item (thinpencil, clipor the like) to center theweighingyokesupportagain.

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Carefullytaketheweighing yokeoutofthesmalltransportcase.Handletheweighingyokeasshownintheoppositefigure(left)andmakesurenottosqueezeit!

Carefullyinserttheweighing yokeintheopeningprovidedinthebaseofthemeasuringunit.Important: Alargeandsmallpinarelocatedatthefootoftheweighingyoke,whichcentertheyokeintheweighingcell.Ensurethatbothpinsareinthecorrespondingholesintheweighingcell.

Carefullyinserttherackintheracksupport.Therackonlyengagescorrectlyinthecamontheracksupportwhenbothslotsareontheright-handside.Wheninsertingtherack,ensurethattheweighingyokeremainsinitsplace.

Placethe12containersintherack.Ensurethattheconicalcenter-ingspigotsofthecontainersareontheoutsideoftheindentedguiderailonbothsides.


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Placetherack coverontherack.Thecamonthesideofthecovermustpoint towards the rightandengage in thecorrespondingslotontherack.

Placetheslideontherackcover.Ensurethatthecamsontheslideengageinthecorrespondingslotsontherack.Theillustra-tionoppositeshowstheslideintheopenstatewithfreeaccesstothecontainers.Tosealthefilleropenings,holdtheslideonthetabandslideittotheleft.

Note:Theslidecanalsobemountedata180°angle,thusenablinguser-friendlyoperationforrightandleft-handedusers.

Levelthemeasuringunit(seesection3.2.3).

3.2.2 Preparing the MCP for the calibration of single-channel pipettesThecalibrationofsingle-channelpipettesrequirestheso-called“single-channel kit”.Youwillfindtherespectivepartsinthesmalltransportcase(seesection2.2).Incasethesepartsarenotincludedinthestandarddeliveryyouwillhavetoorderthesingle-channelkitseparately.

Placethemeasuringunitattheselectedlocation.

Installthecentering ringandtheweighing pan.


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Installthelargeorsmallglass tube holderdependingonyourrequirements,theninserttheappropriateglass tube(thefigureshowsthelargeglasstubeholder).

Installtheevaporation trapandputontheglass cover.

Levelthemeasuringunit(seesection3.2.3).

3.2.3 Leveling the measuring unit

Turnbothleveling screwsonthefrontofthemeasuringunituntiltheairbubbleisinthecenterofthelevel indicator.Note:Anincorrectlyleveledmeasuringunitcanresultinmeasurementerrors.


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3.3 System cablingPlacetheevaluationunitnexttothemeasuringunitandthenplacethemotorcontrolunitontheevaluationunit.Thecomponentsarenowreadytobecabled.Note: For systems that include several MCP, only cable the associated components. In this case, please note the serial numbers on the evaluation unit and the measuring unit. They must be identical.

Cablethecomponentsasfollows:

A Connecttheevaluationunittothemeasuringunit.Todothis,usethe60 cmbalancecablewiththe25-pinconnectors(m/m).

B Connecttheevaluationunittothemotorcontrolunit.Todothis,usethe30cmCANcable(m/m).

C Connect themotorcontrolunit to themeasuringunit.Todothis,usethe60cmcontrolcablewiththe15-pinconnectors(m/m).

D Firstconnectthemotorcontrolunittothepowersupply.Todothis,useonlyoneofthetwoACadapterssupplied(primary:100- 240 VAC,50/60Hz,0.8A;secondary:12VDC,2.25A).Note:Ifthemotorcontrolunitisconnectedtothepowersupplyaftertheevaluationunit,problem-freeoperationofthesystemisnotguaranteed.

E Connecttheevaluationunittothepowersupply(themotorcontrolunitmustalreadybeconnectedtothepowersupply,seeabove).Todothis,useonlytheACadaptersupplied(primary:230VAC,50/60Hz,90mA;secondary:12VAC,1.25A).

F ConnectionofthemotorcontrolunittothePCbymeansofthesuppliedUSB/serialconverter.ThetypeofconnectiontothePCvariesdependingontheMCPsystemtypeandthescopeofdelivery.Forthemoment,werecommendthatyoudonotyetconnecttheMCPtothePCbutfirstfamiliarizeyourselfwiththeoperationoftheMCPinthenextsection.TheconnectiontothePCandthecorrespondingconfigurationaredescribedinsection9.

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Startupandmenusettings

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4 Startup and menu settingsThissectionprovidesinformationaboutstartinguptheMCPandthemenusystem,whichcanbeusedtoadaptyourMCPtotherelevantoperatingconditions.ItisassumedthatallthecomponentsoftheMCPhavebeencabledcorrectly(section3).

4.1 Switching the MCP on and offAssoonastheevaluationunitisconnectedtothepowersupply,theMCPstartsupautomatically.

Toswitchoffthesystem,pressandholddownthe«On/Off»keyuntil“OFF”appearsinthedisplay.Whenyoureleasethekey,thedisplaygoesblank.

Toswitchthesystemonagain,brieflypressthe«On/Off»key.Thestart-upproceduretakesafewsecondsandduringthistimevarioussegmentsofthedisplayshowupbriefly.Assoonastheweightdisplayoramessageappearsinthedisplay,thesystemisreadytooperate.

Werecommendthatyouonlyusethe«On/Off»keytoswitchoff theMCPanddonotdisconnectitfromthepowersupply.Thusthesystemrequiresdoesnotrequireawarm-uptime.However,iftheMCPisdisconnectedfromthepowersupply,youmustwaitat least 1 hourafterswitchingonforthesystemtoreachthermalequilibriumandbereadytooperate.

Oninitialstartup,themessageoppositeappearsinthedisplay.ThismeansthattheMCPrequires basic calibration of the containers in the measuring unit.This calibration isperformedinthemenuthatisdescribedinthesectionsbelow.Note:Brieflypressingthe«Start»keywhilethemessageisdisplayedlaunchesthecontainercalibrationproceduredirectly(thecalibrationprocedureisdescribedinsection4.11).

4.2 Menu overviewInthemenuyoucanadapttheMCPtoyourspecificrequirements,changesettingsandcallfunctions.Themenucontains9menuitems,eachofwhichoffersvariousselectionoptions.

Vibrationadapter Processadapter Repeatability Numberofpipettechannels1)

2ndmeasurementofcontainer11)2)

Containerfilllimit2) Timeintervalforrefe-rencemeasurement2)

Containercalibration2) Resettingtofactorysettings

CHNLS 12 F l - n

OLë14.5 dLt t 5 VES. CAL. rESEt

3 OFF Std

OFF

=O0000e

do CAL

1)ThissettingisusuallyconfiguredviathePCapplicationsoftware.2)Thissettingisnotrequiredforthecalibrationofsingle-channelpipettes,henceitisignored.

Informationaboutusingthemenuandtheindividualmenuitemscanbefoundinthesectionsbelow.


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4.3 Using the menu

Calling the menu

Pressandholddownthe«Menu»keyuntil“D\ENU”appearsinthedisplay.Whenyoureleasethekey,thefirstmenuitemappears(Vibrationadapter).

Selecting the menu items

Brieflypressthe«j»keyinthemenu.Thenextmenuitemappearseachtimethekeyispressed.Afterthelastmenuitem(Resettofactorysettings),thefirstmenuitemisshownagain(Vibrationadapter).

Changing the setting in the selected menu item

Brieflypressthe«f»key.Thenextavailablesettingappearseachtimethekeyispressed.Afterthelastsetting,thefirstsettingisshownagain.Onceyouhaveselectedthedesiredsetting,brieflypressthe«j»keytoswitchtothenextmenuitem(seeabove).

Storing the settings and exiting the menu

Onceyouhavemadeallthedesiredsettings,pressandholddownthe«Menu»keyuntiltheMCPreturnstothenormaloperatingmode.Beforetheweightdisplayappears,theoptiontostorethesettingsisbrieflyconfirmed(“StorEd”).

Exiting the menu without storing the settings

Youcanexitthemenuatanytimebybrieflypressingthe«C»key.Anychangesyouhavemadeinthemenuwillnotbestored.

Note: Ifyoudonotpressanykeysforsometimeinthemenu,theMCPautomaticallyreturnstothenormaloperatingmodewithoutstoringanychanges.

D\ENU

OFF

3

3

CHNLS 4

CHNLS 5

rESEt

StorEd

=O0000e


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4.4 Setting the vibration adapterInthefirstmenuitem(Vibrationadapter),theMCPcanbeadaptedtotheambientconditionsatthepointofinstallation(vibrations,drafts,etc.).

TheMCPispresetforoperationinunstableenvironmentsexworks(setting“3”,symbol-izedbythelargewave).

Ifyouareworkinginastableorverystableenvironment,youcanselectoneofthetwoothersettingsandthusincreasetheoperatingspeedoftheMCP:

Setting“1”(smallwave): Forextremelystableenvironments.

Setting“2”(mediumwave): Forstableenvironments.

3

OFF

Std

4.5 Setting the process adapterTheprocessadaptercanbeusedtoadaptabalancetodifferenttypesofweighing.

AsnormalweighingtasksarenotusuallyperformedbytheMCP,theprocessadapterisswitchedoff(setting“OFF”).We recommend that you do not change this setting as this can adversely affect the measuring performance.

4.6 Selecting repeatabilityThecirculariconforthestabilitydetectorislocatedinthebottomleftcornerofthedisplay.Assoonasthemeasurementresultiswithinthespecifiedlimitvaluesoveracertaintimeinterval,themeasurementresultisconsideredstableandtheiconforthestabilitydetectorfades.Therepeatability(“Repro-Set”)settingisusedtodeterminethetimeintervaloverwhichtheresultmustbewithinthelimitvaluesinorderforittobeconsideredstable.Thebettertherepeatability,thelongerthemeasurementprocesslasts.

TheMCPispresetforstandardrepeatabilityexworks(“Std”),i.e.themeasurementvalueisreleasedasstableveryquickly.Werecommendthatyoudonotchangethissettingsoasnottoprolongthemeasurementprocessunnecessarily.

Inadditiontothefactorysettings,4othersettingsareavailable:

“Good”: Quickreleaseofthemeasurementvalue.

“bEttEr”: Slowreleaseofthemeasurementvalue.

“bESt”: Oncestabilityhasbeenachieved,themeasurementvalueisonlyreleasedifithasnotchangedforafewseconds.

“OFF”: Thefunctionisswitchedoff,eachmeasurementvalueisconsideredstableandreleasedimmediately.

4.7 Selecting the number of pipette channelsThismenuitemisusedtodefinethenumberofchannelsforthepipettetobetested.Thisisusually1,8or12channels.TheMCPispresetfor12-channelpipettesexworks,butanynumberofchannelsbetween1and12canbeselected.

Important:Ifyouselect“1”inthismenuitem,youmustpreparethemeasuringunitforthecalibrationofsingle-channelpipettesasdescribedinsection3.2.2.

CHNLS 12


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4.8 Activating/deactivating the second measurement of container 1TheMCPhasafunctionforasecondmeasurementofcontainer1,whichcanbeactivatedordeactivatedinthismenuitem.Note: This menu item applies to the calibration of multichannel pipettes only.

Second measurement deactivated (factory setting):Thereisnosecondmeasurementofthefirstcontainer(theplaceholder_isinsertedinthedatarecordinsteadoftheweightvalueofthesecondmeasurement,seesection9.3).

Second measurement activated:Oncepipettingiscomplete,theliquidinthefirstcon-tainerisweighedagain(secondmeasurement).Basedonthedifferenceinweightandthetimethathaselapsedbetweenthetwomeasurementstheevaporationratemaybecalculated.Theweightvalueofthesecondmeasurementisaddedtothedatarecordforthecurrentmeasuringcycle(seesection9.3).Important:Thevaluesrecordedfortheindividualpipettechannelsarenotchanged,onlyadditionalinformation(weightvalueofthesecondmeasurement)isavailable,whichcanbeevaluatedifnecessary.

F 1 - n .1

F 1 - n

4.9 Defining the fill limit of the containersThismenuitemcanbeusedtodefinethefilllimitforthe12containersinthemeasuringunit.Assoonasthislimitisreached,the“ED\PtY”levelwarningappearsinthedisplay,whichpromptsyoutoemptythecontainers.Note: This menu item applies to the calibration of multichannel pipettes only.

Thefilllimitissetto14.5 gexworks.Eachtimethe«f»keyispressed,thevaluechangesby0.1 g(settingrange:10.0...16.0 g).Topreventcontainersfromoverflowing,youshouldsetthelimitsothatthereisasafetymargin,whichcorrespondstothelargestvolumetobepipetted(filllimit=16.0 gminusthelargestvolumetobepipetted).

OLë 14.5

4.10 Defining the time interval for reference measurementFollowingeachmeasuringcycle,theMCPusestherecordedvaluesasareferenceforthenextpipettingoperation(thisprocesscorre-spondstotaringonastandardbalance).Thelongerthetimebetweentwomeasuringcycles,thegreaterthedangerthatthereferencevalueswillnolongerbevalidduetoevaporationandthesubsequentmeasurementwillthereforebeinaccurate.Thismenuitemcanbeusedtodefinethetimepermittedbetweentwomeasuringcycles.Ifthistimelimitisexceeded,the“do rEF”messageappearsinthedisplay,whichpromptsyoutodeterminethereferenceagain(bybrieflypressingthe«Start»key).Note: This menu item applies to the calibration of multichannel pipettes only.

Thetimeintervalforreferencemeasurementissetto5(minutes)exworks.Eachtimethe«f»keyispressed,thevaluechangesby1minute(settingrange:1...10minutes).dLt t 5


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4.11 Calibrating the containersOninitialstartup,theMCPpromptsyoutocalibratethe12containersinthemeasuringunitwiththe“do CAL”message.Thefilllimitsofthecontainersarebasedontheemptyweightsofthecontainers(section4.9).Whenthe“do CAL”messageisshowninthedisplay,youmaylaunchthecalibrationprocedurebypressingthe«Start»keybriefly.Themessage“CAL.StArt”confirmsthatthecalibrationprocedurehasstarted(seebelow).However,youmayinitatecontainercalibrationatanytimeviathemenu,asdescribedbelow(thisisrecommendedaftereverychangeoflocation).Note: This menu item applies to the calibration of multichannel pipettes only.

Note: Only perform this calibration operation if you are sure that the containers are empty and dry, otherwise the “ED\PtY” level warning appears too late during pipetting and the containers can overflow.

Ifyouwanttocalibratethecontainers,pressandholddownthe«j»keyuntil:

Themessageoppositeappearsinthedisplay,whichconfirmsthestartofthecalibrationoperation(thecalibrationoperationcanbecanceledatanytimeusingthe«C»key).Therackofthemeasuringunitthenmovesbackwardsstep-by-stepandoneofthecontain-ersisweighedwitheachstep.Youcanfollowtheprogressofmeasurementprocessinthedisplay.Oncethelastcontainerhasbeenweighed,therackmovesbacktotheinitialposition.

Followingsuccessfulcompletionofcalibration,theconfirmationoppositeappearsbrieflyandtheMCPexitsthemenuandreturnstothenormaloperatingmode.

Note: If the“Abort” message appears instead of the confirmation, the calibrationoperationcouldnotbeperformedproperly(e.g.duetoadraftorvibrations)andmustberestarted.

VES. CAL.

CAL.StArt

CAL donE

4.12 Resetting to factory settingsThismenuitemcanbeusedtoresetallmenusettingstothefactorysettings.

Note: All the individual menu settings are lost in the event of a reset. The calibra-tion values of the containers are also deleted and the calibration operation must be repeated with empty containers (section 4.11). We therefore recommend that you only execute a reset if you are sure that you really want to carry out the required calibration with empty containers.

Ifyouwanttoresettothefactorysettings,pressandholddownthe«j»keyuntil:

Theconfirmationoppositeappearsinthedisplay.TheMCPthenexitsthemenuandreturnstothenormaloperatingmode.

Note:Followingreset,the“do CAL”messageappearsinthenormaloperatingmode,whichpromptsyoutocalibratethecontainers(section4.11).

rESEt

r donE

WorkingwiththeMCP

20

5 Working with the MCPThissectionprovidesinformationabouthowtoworkwiththeMCP.ItonlyexplainshowtoworkontheMCP,informationaboutevaluat-ingthedataonthePCandaboutusingtheoptionalPCapplicationsoftwarecanbefoundinthedocumentationsuppliedwiththesoftware.

5.1 Calibrating the weighing cellInordertoensuretheaccuracyofthemeasurementresults,theweighingcellbuiltintointhemeasuringunitmustbecalibratedonthefollowingoccasions:

– Oninitialstartup(allowatleast60minuteswarm-uptimebeforecarryingoutthecalibration)

– Aftereverychangeoflocation

– Whenthesmall“Cal”symbolappearsintheupperpartofthedisplay(theweighingcellispermanentlymonitoredandthe“Cal”symbolappearsautomaticallyassoonasthecellrequiresre-calibration).The“Cal”symboldisappearsuponsuccessfulcompletionofthecalibration.

Regardlessoftheabovecalibrationintervalswestronglyrecommendtocalibratetheweighingcellatleastonceaday.Thecalibrationisperformedwithbuilt-inweights.

SwitchtheMCPonwiththe«On/Off»key.Note:IftheMCPwasdisconnectedfromthepowersupply,waitatleastanhourafterswitchingonforthesystemtoreachthermalequilibrium.

Pressandholddownthe«Cal»keyuntilthedisplayoppositeappears.Whenyoureleasethekey,calibrationbegins.

Youcanfollowtheprogressofthecalibrationoperationinthedisplay:First,shorthorizontalbarsaredisplayedwhilethezeropointisbeingdetermined.Themaximumloadingrams(“100.00000 ”)thenappearsinthedisplay,followedbythezeroload(“0.00000”).Note:Thecalibrationoperationcanbecanceledatanytimebypressingthe«C»key.

SuccessfulcompletionofcalibrationisbrieflyconfirmedwiththemessageoppositeandtheMCPthenreturnstothenormaloperatingmode.

Note:IftheMCPabortsthecalibrationoperationwiththe“Abort”message;unstableambientconditions(draft,vibrations,etc.)areusuallythecause.Clearthemessagebybrieflypressingthe«C»key,makesuretheambientconditionsarestableandrestartthecalibrationoperation.

CAL donE

CAL int

5.2 Preparing the work station

Placethewater reservoirsuppliedonyourworkstationandfill itwithdistilledwater.Waitatleast1hourbeforestartingpipetting.Thisensuresthatthewaterisatambienttemperature.

Note:IfyoudonotworkwiththeMCPforanextendedperiodoftime(e.g.overnight),youshouldplacethecoversuppliedoverthereservoirinordertopreventthewaterbecomingcontaminated.

Thewaterreservoirshouldbereplacedfromtimetotime,asdespitetheuseofdistilledwater,aslimydepositcanformovertimeduetoimpuritiesintheair.Replacementreser-voirscanbeorderedfromMETTLERTOLEDO(section8).

WorkingwiththeMCP

21

5.3 Testing multichannel pipettes

Thedescriptionsinthissectionassumethatthefollowingworkhasalreadybeencarriedout:

– Correctcablingofallsystemcomponents(section3.3)

– Assemblyofthemeasuringunitfortheverificationofmultichannelpipettes(section3.2.1)

– Settingofthenumberofpipettechannels(section4.7)

– Calibrationofthecontainers(section4.11)

– Othermenusettings,ifrequired(section4)

– Calibrationoftheweighingcell(section5.1).

5.3.1 Filling the moisture trapTheMCPisfittedwithamoisturetrap.Themoisturetrapconsistsofsixelongatedcontainersintheracktotheleftandrightofthecontainers.Themoisturetrapisfilledwithdistilledwater,whichslowlyevaporatesandthuscreatesasaturatedatmosphereintherack.Thisatmosphereminimizesevaporationfromthecontainersandthusassociatedmeasuringinaccuracies.

Removetherackcoverandtheslide.Werecommendtoremovethecontainerssothatyouhavefreeaccesstothemoisturetrap.Fillallcontainersofthemoisturetraponbothsideswithdistilledwater.

Note:Fill with water carefully so that no other parts of the MCP become wet. Do not overfill the moisture trap, otherwise the water could spill out when the rack is moved. There is a risk of corrosion. Furthermore, the weighing system is not watertight and the penetration of water into the weighing cell below the rack could damage the MCP.

Placethecontainersbackintherackandreplacethecoverandslide.

Werecommendthatyouchecktheleveloftheliquidinthemoisturetrapregularlyandrefillwithdistilledwater,ifnecessary.

Important note for transport:BeforeyoumovetheMCPtoanotherlocation,removeallthecontainersandemptythemoisturetrap.

5.3.2 Reference measurementNote:IfyouhavealreadystarteduptheMCPforthefirsttime,youarepromptedtocalibratethecontainerswiththe“do CAL”message.First,carryoutthisoperatingstep(foradescriptionsee4.11).

IfyouswitchontheMCPwhenyoustartwork,the“do rEF”messageusuallyappearsfirstandpromptsyoutoperformareferencemeasurement.Duringreferencemeasurement,thecontainersareweighedwiththeircontentsandthemeasurementvaluesareusedasabasis(reference)forthenextmeasurement.Thismessageappearseachtimethetimeintervalspecifiedforreferencemeasure-mentisexceeded(thiscanbedefinedinthemenu,seesection4.10).Thiscanalsooccurduringtheworkingdayifthetimebetweentwomeasurementprocessesexceedsthetimeintervalspecified.However,ifthetimebetweentwomeasurementprocessesiswithinthetimeinterval,the“do rEF”messagedoesnotappear,asthevaluesofthepreviousmeasurementareusedasabasisforthesubsequentmeasuringcycle.

WorkingwiththeMCP

22

Eachtimethe“do rEF”messageappearsinthedisplay,brieflypressthe«Start»keytoperformareferencemeasurement.Note:Referencemeasurementcanbecanceledatanytimeusingthe«C»keyandthenrestarted.

Therackofthemeasuringunitthenmovesbackwardsstep-by-stepandoneofthecontain-ersisweighedwitheachstep.Youcanfollowthemeasurementprocessinthedisplay:Theweightofeachcontainerisdisplayedandassoonasthestabilitydetector(thesmallcircleinthebottomleftcornerofthedisplay)fades,thevalueisaccepted.Oncethelastcontainerhasbeenweighed,therackmovesbacktotheinitialposition.

Followingsuccessfulcompletionofreferencemeasurement,theconfirmationoppositeappearsbrieflyandthen:

TheMCPindicatesthatitisreadyforpipetting.

Note:Ifthe“do rEF”messagereappearsinsteadoftheconfirmation,referencemea-surementcouldnotbeperformedproperly(e.g.duetoadraftorvibrations)oryouhavecanceledthemeasurementwiththe«C»key.Restartreferencemeasurement.

do rEF

rEF donE

rEAdY

5.3.3 Performing the measurement

Makesureagainthatyouhaveselectedthecorrectnumberofchannelsinthemenuforyourpipette(section4.7).

Setthevolumeforthefirstmeasurementprocessatthepipette(e.g.10%ofthenominalvolume).Pleaseobserveanyotherspecificationsforpreparingthepipette(e.g.accordingtoISO8655).

StartthedataacquisitionprogramonthePC.Note:Theconfigurationandoperationofthedataacquisitionprogramarenotdescribedinthissection.IfyouareworkingwiththeoptionalsoftwarefromMETTLERTOLEDO,pleaseobservetheoperatinginstructionssuppliedwithit.

Usethepipettetodrawthesetvolumefromthewaterreservoir,opentheslideontherackcoverandemptythepipetteintothecontainer.Pleasenoteallregulationsregardingcorrectpipetting(e.g.accordingtoISO8655).

Note

– Pipettingmustalwaysbeginwithcontainer1(notethemarking“1”ontherackcover)andtheorientationofthepipettemustnotbechangedduringameasuringcycle(donotturnthepipette!).

– Ifanerroroccursandyouwouldliketorepeatthepipettingprocess,holddownthe«Start»keyforalongtime.Thistriggersanewreferencemeasurement(section5.3.2)andyoucanthenstartpipettingagain.

Ñ 18.04326e

Note:Youmaytriggerareferencemeasurementatanytime(withoutthe“do rEF”messagebeingdisplayed).Thismayberequiredifyouthinkapreviousmeasurementdidnotproducereasonableresults.Inthiscasemakesurethedisplayshows“rEAdY”,thenpressandholdthe«Start»keytolaunchthereferencemeasurement.

WorkingwiththeMCP

23

– Youmayspeedupthemeasurementbyreducingtheresolutionofthemeasuredvaluesto4decimalplaces(insteadof5).Brieflypressthe«1/10»keytotoggletheresolutionbetween4and5decimalplaces.However,thelowresolutionisrecommendedonlyforlarge-volumepipetteswherearesolutionof4decimalplacesissufficient.Note:TheselectedresolutionisnotlimitedtothedisplayofmeasuredvaluesbutalsoaffectsthedatarecordstransferredviatheMCPinterface(section9.3).

Closetheslideandbrieflypressthe«Start»keytotriggerthemeasurementprocess.Note:Themeasurementprocesscanbecanceledatanytimeusingthe«C»key,however,theMCPthenrequiresanewreferencemeasurement.

The rack of the measuring unit then moves backwards step-by-step and one of thecontainersisweighedwitheachstep.Oncethelastcontainerhasbeenmeasured,therackreturnstotheinitialposition.Note:Ifyouhaveactivatedthesecondmeasurementofcontainer1inthemenu(section4.8),thefirstcontainerisweighedasecondtimetocompletethemeasurement.

Successfulcompletionofthemeasurementisconfirmedbrieflywiththemessageopposite.The“rEAdY”messagethenappearsandtheMCPisreadyforthenextmeasurement.donE

5.3.4 Emptying the containers

ED\PtYAssoonasoneofthecontainershasreachedthefilllimitspecifiedinthemenu(section4.9),therequestoppositetoemptythecontainersappears.

ConnectthesuctionpumpsuppliedtothepowersupplyviatherelevantACadapter.Note:Anoptional4-channelsuctionpumpisavailabletoincreaseefficiencywhenemptyingthecontainers,seesection8.8.

Connectthesuctiontube(withtheprobe)totheinput(“IN”)onthepump.Connectthesecondtubetotheoutputonthepump(“OUT”)andplacethefreeendinasuitablecollector.

Carefullyinsertthesuctionprobeinthefirstcontainer.Pressthe“ON”switchonthepumpandemptythecontainer.Thisonlytakesafewseconds.Repeatthisprocessuntilallthecontainershavebeenemptied.

Note:Do not allow the pump to run dry for too long as it may become damaged. When handling the probe, make sure that no other parts of the MCP become wet. There is a risk of corrosion. Furthermore, the weighing system is not watertight. The penetration of water into the weighing cell below the rack can damage the MCP.

Thenext timethe«Start»key ispressed, theMCPrechecksthe liquid level. If there isstillmorethan2 mlofliquidinoneofthecontainers,the“ED\PtY”messageappearsagain.

WorkingwiththeMCP

24

5.4 Testing single-channel pipettes

Thedescriptionsinthissectionassumethatthefollowingworkhasalreadybeencarriedout:

– Correctcablingofallsystemcomponents(section3.3)

– Assemblyofthemeasuringunitfortheverificationofsingle-channelpipettes(section3.2.2)

– Settingthenumberofpipettechannelsto“1”(section4.7)

– Calibrationoftheweighingcell(section5.1).

5.4.1 Filling the moisture trap

Removetheglasscoverfromtheevaporationtrap.

Filltheevaporationtrapwithdistilledwater.

Note:Fill with water carefully so that no other parts of the MCP become wet. There is a risk of corrosion. Furthermore, the weighing system is not watertight and the penetration of water into the weighing cell below the rack could damage the MCP.

Putontheglasscoveragain.

Werecommendthatyouchecktheleveloftheliquidinthemoisturetrapregularlyandrefillwithdistilledwater,ifnecessary.

5.4.2 Performing the measurement

Setthevolumeforthefirstmeasurementprocessatthepipette(e.g.10%ofthenominalvolume).Pleaseobserveanyotherspecifi-cationsforpreparingthepipette(e.g.accordingtoISO8655).

StartthedataacquisitionprogramonthePC.Note: Theconfigurationandoperationofthedataacquisitionprogramarenotdescribedinthissection.IfyouareworkingwiththeoptionalsoftwarefromMETTLERTOLEDO,pleaseobservetheoperatinginstructionssup-pliedwithit.

WorkingwiththeMCP

25

Drawthesetvolumefromthewaterreservoir,thenemptythepipetteintotheglasstube.Pleasenoteallregulationsregardingcorrectpipetting(e.g.accordingtoISO8655).

Themeasuredweightvalueappearsinthedisplay.Pressthe«Start»keytotransferthevalueviatheinterface.Threeconcentriccirclesaredisplayedatthetopedgeofthedisplaywhiledatatransferisinprogress.Assoonasthethreecirclesdisappear,datatransferiscompleteandtheMCPisreadyforthenextmeasurement.

Note

– Pressingthe«Start»keytransmitsthedisplayedvalueviatheinterface.Thisvalueiscumulative,i.e.theMCPcontinuouslysumsupallmeasurements.Incaseyourparticulardataacquisitionprogram requires theweight valueofevery singlemeasure-mentyouwillhavetoresettheMCPdisplaytozero(withthe«k»key)beforestartingthenextmeasurement.

– Youmayspeedupthemeasurementbyreducingtheresolutionofthemeasuredvaluesto4decimalplaces(insteadof5).Brieflypressthe«1/10»keytotoggletheresolutionbetween4and5decimalplaces.However,thelowresolutionisrecommendedonlyforlarge-volumepipetteswherearesolutionof4decimalplacesissufficient.Note:Theselectedresolutionisnotlimitedto the display of measured values but also affects the datarecordstransferredviatheMCPinterface(section9.3).

0.02016e

Statusanderrormessages

26

6 Status and error messagesTheMCPdisplaysmessagestoinformyouaboutthecurrentinstrumentstatus,actionstobecarriedoutandanyerrors.

Message Meaning Action required/note

rEAdYThe MCP is ready for the next measure-ment

None

donEThemeasurementwassuccessfullycom-pleted.

Themessagedisappearsaftera fewseconds.

do rEFReferencemeasurementrequired Performreferencemeasurement(sec-

tion5.3.2).Ifreferencemeasurementdoesnotstartandthemessagereap-pears, check the hardware (correctinstallation of the weighing yoke,containers,moisture in theweighingsystem,etc.).

ED\PtYContainerfilllimithasbeenreached. Emptycontainers(section5.3.4).

do CALBasiccalibrationofthecontainersrequired.

Themessageonlyappearsoninitialstartupandwhenthemenusettingshavebeenresettothefactorysettings.Perform basic calibration (section4.11).

no FuncYouhavepressedakey,whichdoesnothaveafunctioninthiscontext.

Pressthecorrectkey.

AbortA measurement or calibration operation(calibrationof the containersor internalcalibrationoftheweighingcell)couldnotbecompletedproperly.

Makesuretheambientconditionsarestableandrestartthemeasurementorcalibrationoperation(section4.11and5.1).Ifnecessary,changethelocationoftheMCP.

wssssxOverload Check that the weighing yoke is in-

stalledcorrectly(sec.3.2.1).Iftherackisintheinitialposition,noweightmaybeplacedonthecantileverarm.

y____zUnderload Check that the weighing yoke is in-

stalledcorrectly(section3.2.1).

Statusanderrormessages

27

Message Meaning Action required/note

The MCP is searching for the zero point(waitingtoreachstability).

None

rEF Err.Youhaveattemptedtostartameasurementeven though a reference measurementmustfirstbeperformed.Thismessageonlyappears if youcontrol theMCPviaSICScommandsfromaPC(section9).

First,performareferencemeasurement(viatheMCPkeypadorwiththerelevantSICScommand)andthenrestartthemeasuringcycle.

Cal Theweighingcellrequirescalibration. Calibrate the weighing cell as soonas possible (section 5.1).The“Cal”symbol disappears upon successfulcompletionofthecalibration.

Cleaningandservice

28

7 Cleaning and serviceCleanallthecomponentsoftheMCPeveryonceinawhileusingaslightlydampenedcloth.Ifnecessary,useacommerciallyavailable,mildcleaningagent.Tocleanthemeasuringunitthoroughly,proceedasfollows:

DisconnecttheACadapterofthemeasuringunitfromthepowersupply.

Unplugalltheconnectorsfromthebackofthemeasuringunit.

Removetheslideandtherackcover.

Carefullyliftall12containersfromtherack.Caution: The glass tubes are fragile!

Carefullylifttherackverticallyfromtheracksupport.

Removetheweighingyoke.

Lifttheracksupportoutofthemeasuringunit.

Cleanallthepartsusingaslightlydampenedcloth.Note:Makesurethatnomoisturepenetratestheweighingcell.Ifnecessary,covertheweighingyokeopeningwithadhesivetape.

Forcleaningpurposestheglass tubes of the containersmayberemovedasfollows:Slidethesuppliedrubberconeovertheglasstubeandwithdrawthetubefromthecontainer.Aftercleaning,drytheoutsideoftheglasstubeandthetubeguideofthecontainercovercarefully(duetocapillaryactionincreasedevaporationmayoccurifthesepartsarewet)!Relocatetheglasstube.Note:Theglasstubesshouldbefirmlyseatedthustheycannotberemovedbyhand.Incaseaglasstubeisnotfirmlyseated,removeitandthenslightlysqueezethecontaineronbothsides.Relocatetheglasstubeandcheckforproperseatingagain.Aftercleaning,replaceallthepartsinthemeasuringunit(section3.2.1).Thencheckwhetherthemeasuringunitisstillleveledcor-rectly(section3.2.3).Reconnectthemeasuringunittotheevalu-ationunitandthemotorcontrolunit.ConnecttheACadaptertothemeasuringunitandconnectthemeasuringunittothepowersupply.

Important notes

– Neverusecleaningagents,whichcontainsolventsorabrasiveingredients.Suchagentscancausescratchedsurfaces,especiallyinthecaseofthecoverglassofthedisplayontheevaluationunit.

– MakesurethatnoliquidpenetratestheindividualcomponentsoftheMCPorcomesintocontactwiththeconnectionsonthebackofthehousing.

– NeveropenthehousingofMCPcomponents,theydonotcontainanypartsthatcanbecleaned,repairedorreplacedbytheuser.

AskyourMETTLERTOLEDOdealerabouttheserviceoptionsavailable–regularservicingbyanauthorizedservicetechnicianensuresmeasuringaccuracyforyearsandprolongstheservicelifeofyourMCP.

Technicaldataandaccessories

29

8 Technical data and accessoriesThissectioncontainsthetechnicaldataoftheMCPandinformationaboutoptionalaccessoriesthatarecurrentlyavailable.Informationaboutstandardequipmentcanbefoundinsection2.

8.1 MCP105 data

Number of channels: 1...12

Volume of the containers: 16 ml(levelwarningconfiguredinthemenu)

Measuring time for 8/12 channels: 70 s/110 s(incl.repeatedmeasurementofthefirstcontainer)

Moisture trap: Built-in

Evaporation rate calculation : Byremeasuringthefirstcontainer,canbeactivatedviathemenu

Minimum measuring volume: 10 µl(formeasurementsaccordingtoISO8655)

Weighing module data (built into the measuring unit):

Readability: 0.01 mg

Max. load: 101 g

Measurement uncertainty: 0.02 mg

Stabilization time: approx.5 sec

ThisdataisonlyvalidiftheMCPisoperatedwithinthespecifiedambientconditions(section8.2).

8.2 Ambient conditionsProblem-freeoperationoftheMCPandcompliancewiththeweighingmoduletechnicalspecifications(section8.1)areonlyensuredunderthefollowingambientconditions:

Installation location: Closed,dryrooms.Operationinhazardousenvironmentsisnotpermitted.

Height above sea level: Upto4000 m

Ambient temperature: 5–40 °C(41–104 °F)

Relative atmospheric humidity: Max.80 %at31 °C,lineardecreaseofupto50 %at40 °C,non-condensing

Warm-up time: 60minutes,minimumfollowingconnectiontothepowersupply(iftheevaluationunitandmotorcontrolunitarepermanentlyconnected to thepowersupply, theMCPrequiresnowarm-uptimeafterswitchingonwiththe«On/Off»keyandisim-mediatelyreadytooperate).

8.3 Protection and standards

Installation category: Class2

Pollution degree: 2

Protection: Protectedagainstdustandwater

Safety and EMC: Seedeclarationofconformity(separatebrochure11780294)


30

8.4 Power supplyOnly the AC adapters suppliedshouldbeusedtosupplypowertotheevaluationunit,motorcontrolunitandsuctionpumpwithcountry-specificpowercablesand3-pinconnectors.Themeasuringunitdoesnotrequireconnectiontothemains,itissuppliedbytheevaluationunitandthemotorcontrolunit.

AC adapter for the evaluation unit (suppliedwithwallmountingplate)

OneofthefollowingACadaptersissupplieddependingonthedestinationcountry:

Primary: 115 VAC(-20/+15%),50/60Hz,195mA, secondary:12VAC,1.25A

Primary: 230 VAC(-20/+15%),50/60Hz,90mA, secondary:12VAC,1.25A

AC adapter for the motor control unit and suction pump

Primary: 100-240 VAC(-15/+10%),50/60Hz,0.8A

Secondary: 12 VDC(±5%),2.25Amax.(electronicprotectionagainstoverload)

8.5 RS232C interface dataThebuilt-inRS232CinterfaceinthemotorcontrolunitisusedastheMCPconnectiontoaPC(directorviaUSB/serialconverter).Theinterfacehasthefollowingspecifications:

Interface type: Voltage-controlledinterfaceaccordingtoEIARS-232C/DIN66020(CCITTV.24/V.28)

Max. cable length: 15 m

Signal level: Outputs: Inputs:

+5 V...+15 V(RL=3-7 kΩ) +3 V...25 V

-5 V...-15 V(RL=3-7 kΩ) -3 V...25 V

Connection: 9-pinD-SUB,female

Operating mode: Fullduplex

Transmission mode: Bit-serial,asynchronous

Transmission code: ASCII

Baud rates: 600,1200,2400,4800,9600(factorysetting)1)

Bits/parity: 7bit/even,7bit/odd,7bit/none,8 bit/none(factorysetting)1)

Stop bits: 1 stop bit(factorysetting)2),2stopbits

Handshake: None,XON/XOFF(factorysetting),RTS/CTS1)

End of line: <CR><LF>2)

Pin assignment: Pin2:MCPtransmittingline(TxD)

Pin3:MCPreceivingline(RxD)

Pin4:Readytoreceive(hardwarehandshake)(DTR)

Pin5:Signalground(GND)

Pin6:Cleartosend(hardwarehandshake)(DSR)

1) FactorysettingcanbemodifiedbyMETTLERTOLEDOservicetechnicians2) Settingcannotbemodified

6

15

9

DataGND


31

8.6 Dimensions and weights

Evaluation unit and motor control unit

Weightofmotorcontrolunit: 0.6 kg

Weightofevaluationunit: 1.2 kg

Measuring unit

Weightofmeasuringunit: 6 kg

220

mm 91

mm

177 mm

296

mm

160 mm

88 mm

171 mm

164

mm

Technical data and accessories

32

8.7 MCPtechnicalspecificationsThe following table contains the times measured by METTLER TOLEDO for testing a pipette on systems consisting of 1 to 5 MCP. De-pending on the MCP model, the relevant environment and the work technique, the measuring times may in practice deviate from those provided.

To calculate the measuring times, the following two test methods were used that are based on the most commonly used test meth-ods:

– MethodA: 10measurements each at 10 %, 50 % and 100 % of the nominal volume of the pipette, this complies with the require-ments of ISO 8655.

– MethodB: 4measurements each at 10 %, 50 % and 100 % of the nominal volume of the pipette.

NumberofMCP–> 1*MCP 2*MCP 3*MCP 4*MCP 5*MCP

Method Channels

A 12 59 30 21 17 14

A 8 51 26 18 15 12

B 12 24 13.5 10 8 7.5

B 8 21 11.5 8.5 7 6.5

All measuring times are specified in minutes, based on measurements made with the MCP105 model.

Assumedhandlingtimerequiredtodeterminethemeasuringtimes

Multichannel pipettes: Per pipetting operation 10 seconds

Volume change: Per change 10 seconds

8.8 Accessories

Designation Articleno.

“Calibrysoftware” (Microsoft Windows®) with operating instructions, singleworkstationversion 11138419

“Calibrysoftware” (Microsoft Windows®) with operating instructions, singleworkstationversionLight 11138423

“Calibrysoftware” (Microsoft Windows®) with operating instructions, network-compatibleversion 11138420

CalibryUpdate 11123915

ProgramCasetteMCP 11138300

Calibrationkitforcontrolofinspection,measuringandtestequipment (calibration of the weighing module using external weights)

11138254

4-channelsuctionpump for emptying the containers 11138252

Single-channelkit100µl 11138008

Single-channelkit20ml 11138006

WorkingtableMCP5 (140 x 140 x 78 cm) 11138043

WorkingtableMCP2 (80 x 80 x 78 cm) 11138040


33

8.9 Spareparts

Designation Articleno.Waterreservoir(asareplacementforthewaterreservoirsupplied),setof5pcs 11600616Watercontainer(includingglasstube) 11138266Glasstubes(forthewatercontainers),setof5pcs 11138253Slideforrackcover 11138123Rackcover 11138121Rack 11138265Supportforevaluationunit 11138024Sparepartsforsingle-channelpipettes Glasscover

Evaporationtrap

Glasstubesmall15mmdiameter

Glasstubeholderfor15mmglasstube

Glasstubelarge24mmdiameter

Glasstubeholderfor24mmglasstube

Weighingpan

Centeringring

00210863

00210862

00210864

00210870

00210865

00210869

11138259

00210872CablesPoweradaptorformotorcontrolunitandsuctionpump

ACadaptorforevaluationunit

PowercablesfortheACadapters DK GB USA AUS SA Euro(grd) CH I

1“BalanceCable”(connectioncableforevaluationunit/measuringunit60cmlong,25-pin,m/m)

1“CANCable”(connectioncableforevaluationunit/motorcontrolunit30cmlong,m/m)

1“ControlCable”(connectioncableformotorcontrolunit/measuringunit60cmlong,15-pin,m/m)

1USB/serialconverter

USBHub

11107909

00224570

0008745200089405000886680008875100089728000879250008792000087457

00211535

00239259

11138403

11103691

11600611Single-channelsuctionpump 11138268Tubeforsuctionpump 11138132Largetransportcase 11138351Smalltransportcase 11138350

Appendix:MCPandhostcomputer

34

9 Appendix: MCP and host computerThissectionprovidesinformationaboutconnectingoneormoreMCPtoahostcomputer(PC),settingupanetworkwithseveralMCPandtheSICScommandsthataresupportedbytheMCP.

Caution: Do not place the PC keyboard on the same working surface as the MCP! Using the keyboard produces vibrations which may cause the MCPxxx to abort a running action (such as a reference mea-surement). The MCP then requires to perform the appropriate action again before further commands can be processed.

9.1 Connection to the host computerTheMCPsystemisusuallyconnectedtothehostcomputer(PC)viaaUSB(UniversalSerialBus)andallpartsrequiredforthisareincludedinthescopeofdelivery.Inprinciple,adirectconnectiontotheRS232Cinterface(s)onthecomputerisalsopossible.AnRS232CinterfacemustthereforebeavailableonthecomputerforeachMCP.Thevariousoptionsforconnecting via USBareexplainedbelow.

ThePCconnectionisdoneviathe9-pinRS232Cinterfaceconnectorlocatedonthebackpanelofthemotorcontrolunit.

System consisting of a single MCP, supplied with a USB/se-rial converter

Insert the 9-pin connector of the USB/serial converter into theRS232Cinterfaceofthemotorcontrolunit.

InserttheUSBcableoftheconverterinafreeUSBinterfaceonyourPC.

System consisting of several MCP, supplied with several USB serial converters and a USB hub

Insert the9-pinconnectorof theUSB/serialconverters into theRS232Cinterfaceofthemotorcontrolunits.

ConnecttheUSBcableofeachconvertertotheUSBhub.

ConnectthehubtoafreeUSBinterfaceonyourPC.

MCPMotorcontrolunit

PC

USB/serialconverter

USBcable

RS232C9-pUSB

MCPMotorcontrol

unit1

PC

USB

cabl

e

RS232C9-pUSB

USBHubUSBcable

USB/serialconverter

MCPMotorcontrol

unitnRS232C9-p

USB/serialconverter

USBcable


35

System consisting of several MCP, supplied with a USB hub with an integrated USB/serial converter

ConnectthefreeendoftheRScableofeachMCPmotorcontrolunitdirectlytoanRSconnectiononthehub.

ConnectthehubtoafreeUSBinterfaceonyourPC.

MCPMotorcontrol

unit1

PCUS

Bca

ble

RS232C9-pUSB

MCPMotorcontrol

unitn

USBhubwithintegratedUSB/serialconverter

RScable

RS232C9-p

RS23

2C9

-p

RScable

9.2 PC softwareThenetwork-compatibleandvalidatable“Calibry” softwareavailablefromMETTLERTOLEDOcanbeusedonthePCasanoption.Alternatively,theMCPcanbeintegratedintoindividual(existingorspeciallycreated)applications.Thissectionbrieflyexplainsthefunc-tionsofthesoftwareandprovidesanoverviewoftheinteractionbetweentheuser,MCPandsoftware.

9.2.1 PC software functionsInprinciple,thePCsoftwarecarriesoutthefollowingtasks:

– ConfiguringtheconnectedMCP

– Distinguishingbetweendataandcontrolcommands

– AssigningCOMportstoindividualmeasurementproceduresandcancelingtheassignmentfollowingcompletionofthemeasure-ment(thereservationofCOMportsisdescribedbelow)

– Startingreferencemeasurements(usingSICScommands,seesection9.3)

– Monitoringthenumberofpipettingoperationscompletedandstillinprogress

– Storingmeasurementdata

ThePCsoftwareisnotusedtocontrolthemeasurementprocedure,thisisexecutedbytheMCPalone(thePCsoftwarecanonlytriggerameasurement/referencemeasurementorcancelaprocessthatisinprogress).ThePCsoftwaredoesnotrequestanydata,thisistransferredindependentlybytheMCP.

Thediagramsinthesubsequentsectionsshowtheworkflowuponinitializationandconfigurationofthemeasuringsystem,therefer-encemeasurement,andthemeasurementofpipettes.Pleasenotethefollowing:

– ThediagramsshowjustoneMCP.IfasystemincludesseveralMCPxxx,thecommandsaresenttoeachunit.

– Spacesaredesignatedwith“_”,“<CRLF>”meansASCIIcharacters0x0D0x0A,<TAB>meansASCIIcharacter0x09.

– Allvaluesshowninthediagramsareexamples.


36

9.2.2 Initialization and configuration of the measuring systemThefollowingillustrationshowstheinteractionbetweentheuser,PCsoftwareandMCPwheninitializingandconfiguringthemeasuringsystem.

MCP Controller MCPxxxMCP Operator

1: start MCP controller

2: open serial port

3: request device type ( I2<CRLF>)

4: return device type

5: request software versions (I3<CRLF> )

6: return software versions

8: return serial number

7: request serial number (I4<CRLF>)

9: return status

10: configure measurement

11: configure (e.g. A107_12_0<CRLF>)

12: confirm (A107_A<CRLF>)

13: return status

14: start reference measurement

15: start reference measurement (A109<CRLF>)

19: reference measurement done (A109_A<CRLF>)

20: return status

18: do reference measurement

16: reference measurement started (A109_B<CRLF>)

17: return status

21: display status 'READY'

(e.g. I2_A_"MCP105_101.00090_g"<CRLF>)

(e.g. I3_A_"1.66_1.00_26080842"<CRLF>)

(e.g. I4_A_"1113153280"<CRLF>)


37

9.2.3 Measurement procedureThefollowingillustrationshowsthesequenceofameasurementprocess.


1: dispense desired volume

21: display status 'READY'()

6: measure channel #1()

2: press 'START'

3: measurement started (A108_B<CRLF>)

7: return channel #1 weight (e.g. 0.00015<TAB>)( )


9: return channel #2 weight (e.g. 0.00014<TAB>)()


10: ...channels #3..#7...

12: return channel #8 weight (e.g. 0.00013<TAB>)( )

14: return channel #1 check weight (e.g. 0.00012<TAB>)()

15: return weight unit (g<TAB>)()

16: return place holder (_<TAB>)()

17: return place holder (_<TAB>)()

18: return serial number (1113153280<CRLF>)()

19: measurement done (A108_A<CRLF>)( )

13: measure channel #1 again()

20: return status()

5: return status()4: make COM port reservation()


38

The“Make COM port reservation”functionisthemostimportantfunction.Itcanbeusedtomanagedatafromanunknownnumberofinstruments.

Example:ThreeMCPareconnectedtothecomputer(toCOMports8,9and10)and3volumeswith10measurementseacharetobetaken.ThemeasurementcounterissettozerointhePCsoftware.

Theuserpressesthe«Start»keyonthefirstinstrument(atCOMport9).Theinstrumentsendsthe“A108B”confirmation.Thisincreasesthemeasurementcounterbyone.Alldatathatisthenreceivedatport9isassignedtothe“Index=Measurementcounter=1”measur-ingdataarea.Therefore,port9isconnectedto“Index=1”.Note:TheMCPsuppliesthemeasurementdataautomaticallytothehostinterface.Thedataistransferredcontinuously,whichinturnenablescontinuousmonitoring.

Theuserthenstartsthemeasurementonthesecondinstrument(COMport10).Thismeansthatthemeasurementcounterincreasesbyoneagain.Alldatathatisthenreceivedatport10isassignedtothe“Index=Measurementcounter=2”measuringdataarea.

If theMCPatport9 sendsan“A108 A”confirmation (“Measurement completed”, see section9.4.3), theconnectioncanbe re-moved.

IftheuserthenstartsanothermeasurementontheinstrumentatCOMport9,themeasurementcounterincreasesbyoneagainandthereisanewconnectionfromport9to“Index=3”.

9.2.4 Aborting a reference measurementThefollowingillustrationsshowthesequencewhenareferencemeasurementisaborted,eitherviathe«C»keyoftheMCPorviaanexternalcontroller.

Aborting a reference measurement with the «C» key of the MCP evaluation unit



7: abort measurement

8: reference measurement aborted (A109_I<CRLF>)

abort is executed immediately

10: display status 'DO REF'

9: return status

4: return status




6: press 'C'


39

Aborting a reference measurement via an external controller (external software)


12: reference measurement aborted (A109_I<CRLF>)

6: abort reference measurement


13: return status

8: stopping (A106_B<CRLF>)

7: stop (A106<CRLF>)

11: stopped (A106_A<CRLF>)

4: return status

9: return status


10: abort reference measurement





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9.2.5 Aborting a pipette measurementThefollowingillustrationsshowthesequencewhenapipettemeasurementisaborted,eitherviathe«C»keyoftheMCPorviaanexternalcontroller.

Aborting a pipette measurement with the «C» key of the MCP evaluation unit



5: measure channel #1

2: press 'START'


6: return channel #1 weight (e.g. 0.00015<TAB>)


9: measurement aborted (A108_I<CRLF>)

7: press 'C'

abort is executed immediately


10: return status

4: return status


41

Aborting a pipette measurement via an external controller (external software)



5: measure channel #1

2: press 'START'


6: return channel #1 weight (e.g. 0.00015<TAB>)


11: measurement aborted (A108_I<CRLF>)



13: return status

8: stopping (A106_B<CRLF>)

7: stop (A106<CRLF>)

12: stopped (A106_A<CRLF>)

4: return status

9: return status


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9.3 Data formatThefollowingexampleshowsatypicaldatarecordfortheMCP.Thisdatarecordresultedfromthemeasurement of an 8-channel pipettewithanadditionalsecondmeasurementofthefirstcontainer.

0.00015 0.00014 0.00016 0.00014 0.00016 0.00023 0.00015 0.00013 0.00012 g _ _ 1113153280

1 2 3 4 5 6 7 8 a b c d e

Value Meaning

1...n Weightvaluesoftheliquidpipettedinthecontainers(n=numberofpipettechannels,inthisexamplen=8)

a Weightvalueofthesecondmeasurementofcontainer1

b Unitofmeasurementofweightvalues(alwaysgram)

c Reservedforfutureuse,theplaceholder“_”isenteredinsteadofavalue

d Reservedforfutureuse,theplaceholder“_”isenteredinsteadofavalue

e SerialnumberoftheMCPfromwhichthedataistaken

Note

– AllvaluesareseparatedbytabsandthedatarecordisterminatedbyCRLF.

– Thenumberofdecimalplacesoftheweightvalues(4or5)dependsontheresolutionsettingoftheMCPwhichcanchangedwiththe«1/10d»keyorviaaspecificSICScommand(section9.4.3).Ifaparticularmeasurementandtheassociatedreferencevalueshaveadifferentnumberofdecimalplaces,thecorrespondingdatarecordwillalwaysbebasedonthelowernumberofdecimalplaces.

– Ifthesecondmeasurementofcontainer1wasdeactivatedinthemenu(section4.8),item“a”doesnothaveavalue.Inordertoensureuniformformattingofthedatarecords,theplaceholder“_”isenteredinstead.

9.4 MT SICS commandsThePCsoftwareandMCPinteractviaMTSICScommands,whichcanbeintegratedintoindividualapplications(MTSICS=METTLERTOLEDOStandardInterfaceCommandSet).

TheMCPsupportsarangeofstandardSICScommands,whicharelistedbrieflybelow(section9.4.2).Detailedinformationaboutthesecommandscanbefoundinthe“MT SICS Reference Manual”brochure(onlyavailableinEnglish,00705184).

Inadditiontostandardcommands,specific SICS commands also exist for the MCP.Thesecommandsarenotincludedinthe“MTSICSReferenceManual”butareexplainedindetailinsection9.4.3below.

9.4.1 Notes on the command formatSICScommandsconsistofoneormorecharactersfromtheASCIIcharacterset.Pleaseobservethenotesbelow:

• Enterthecommandusinguppercaselettersonly.

• Thepossibleparametersofthecommandmustbeseparatedfromoneanotherandfromthecommandnamebyaspace(ASCII32decimal,shownhereasï).

• EachcommandmustbeterminatedwithCRLF(ASCII13decimal,10decimal).


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9.4.2 Standard SICS commandsTheMCPsupportsthefollowingcommandsfromthestandardSICScommandset:

– CommandsaccordingtoSICS Level 0: “I0”,“I1”,“I2”,“I3”,“I4”,“S”,“SI”,“SIR”,“Z”,“@”.

– CommandsaccordingtoSICS Level 1:“D”,“DW”,“K”,“SR”,“T”,“TA”,“TAC”,“TI”.(Note:Ifameasurementisinprogresswhilethe“D”commandisreceived,thetextwillappearintheMCPdisplayonlyafterthemeasurementhasbeencompleted).

– CommandsaccordingtoSICS Level 2: “C3”,“I11”.

Detailedinformationaboutthesecommandscanbefoundinthe“MT SICS Reference Manual”(onlyavailableinEnglish,00705184).

9.4.3 Specific SICS commands for the MCP

A105 – Position the rack and request the status

Command A105ïPosition Position:0...13 Targetposition,towhichtherackshouldbemoved(0=startposition,13=endposition).

Response A105ïB Commandisexecuted(theresponseisrepeatedeachtimeapositionispassed).

A105ïA Targetpositionreached.

A105ïL Incorrectnumberofparametersorparametersoutsidethepermis-siblevaluerange(0...13).

A105ïI Commandcannotbeexecuted(e.g.becauseapositioningcommandorameasurementprocessisalreadyinprogressorbecausethereisanerror).

Command A105 Requestthestatusoftherack.

Response A105ïAïPositionïError Position:0...13 Normalpositions99 Intermediateposition

Error:0 Noerror1 Initializationnotyetcompleted2 Incrementpulsegeneratortime-out3 Limitswitcherror

A105ïI Commandcannotbeexecuted(e.g.becauseapositioningcommandorameasurementprocessisinprogress).

A106 – Stop

Command A106 Stoptherackimmediately.Anymeasurementprocessthatisinprog-ressisthusaborted.

Response A106ïB Commandisexecuted.

A106ïA Drivestopped.

A106ïI Commandcannotbeexecuted.


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A107 – Configure the measurement procedure and request the current configuration(seealsosection4.7and4.8)

Command A107ïChannelsïMeas. mode Channels:1...12 Numberofchannelsofthepipettetobetested

Measuring mode(secondmeasurementofcontainer1,seesection4.8):0 Measurementwithsecondmeasurementofcontainer11 Measurementwithoutsecondmeasurementofcontainer1

Response A107ïA MCPconfiguredsuccessfully.


A107ïI Commandcannotbeexecutedatpresent(e.g.becauseameasurementprocessisinprogressorbecausetheMCPmenuisopen).

Command A107 Requestcurrentconfiguration.

Response A107ïAïChannelsïMeas. mode Channels:1...12 Numberofchannelsofthepipettetobetested

Measuring mode(secondmeasurementofcontainer1):0 Measurementwithsecondmeasurementofcontainer11 Measurementwithoutsecondmeasurementofcontainer1

A107ïI RequestcannotbeexecutedastheMCPmenuisopen.

A108 – Start measurement process

Command A108 Startmeasurementprocess.

Response A108ïB Measurementprocessandcontinuoustransferofthemeasuredvaluesstarted(thedataformatisdescribedinsection9.3).

Note:Thisresponseisalsosentwhenthemeasurementwasinitiatedviathe«Start»keyoftheMCP(alsoseediagraminsection9.2.3).

A108ïA Measurementprocesscomplete.

A108ïI Commandcannotbeexecuted(e.g.becauseapositioningcommandorameasurementprocessisalreadyinprogressorbecausethereisanerror,orthemeasurementprocesswasaborted).

A109 – Start reference measurement

Command A109 Startreferencemeasurement.

Response A109ïB Referencemeasurementstarted,continuoustransferofmeasuredvalues

A109ïA Referencemeasurementcomplete.

A109ïI Commandcannotbeexecuted(e.g.becauseapositioningcommandorameasurementprocessisalreadyinprogressorbecausethereisanerror,orthereferencemeasurementwasaborted).


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A111 – Time remaining until next reference measurement

Command A111 Inquiryoftimeremaininguntilnextreferencemeasurement(duringthistimetheunitwillbeavailableforameasurement).

Response A111ïValue Value:Time[s]remaininguntilnextreferencemeasurement:Value>0:Unitisavailableformeasurementforxxseconds.Value<0:Unitisnotavailableformeasurementsincereferencemea-surementisoverdueforxxseconds.

A112 – Start measurement process and supply data in MT-SICS A format

Command A112 Startmeasurementprocess.

Response A112ïx1ïx2ïx3ïx4ïx5 x1 State of measurement process:A Measurementprocesscomplete,lastvalueB Measurementinprogress,furthervaluestofollow

x2 Value identifier:0 Measurementprocessstarted1 Firstmeasurement2 Secondmeasurementforthecalculationoftheevaporationrate3 Evaporationrate(currentlynotused)4 Serialnumberoftheunitperformingthemeasurement

x3 Consecutive number of measurement (channel) (availableonlywithvalueidentifiers1and2)

x4 Measured value (availableonlywithvalueidentifiers1,2and3)

x5 Unit of measured value (availableonlywithvalueidentifiers1,2and3)

Example for 8-channel measurement including the calculation of the evaporation rate

Example A112 Startthemeasurementprocess A112ïBï0 Measurementprocessstarted A112ïBï1ï1ï0.00015ïg Firstmeasurementofchannel1,result0.00015 g A112ïBï1ï2ï0.00014ïg Firstmeasurementofchannel2,result0.00014g A112ïBï1ï3ï0.00016ïg Firstmeasurementofchannel3,result0.00016g A112ïBï1ï4ï0.00014ïg Firstmeasurementofchannel4,result0.00014g A112ïBï1ï5ï0.00016ïg Firstmeasurementofchannel5,result0.00016g A112ïBï1ï6ï0.00023ïg Firstmeasurementofchannel6,result0.00023g A112ïBï1ï7ï0.00015ïg Firstmeasurementofchannel7,result0.00015g A112ïBï1ï8ï0.00013ïg Firstmeasurementofchannel8,result0.00013g A112ïBï2ï1ï0.00012ïg Secondmeasurementofchannel1,result0.00012g A112ïAï4ï"1112345678" Measurementprocesscomplete(“A”),serialnumberofunit.


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A113 – Configure the time interval for reference measurement(refertosection4.10)

Command A113ïTime Time:1...10 maximumadmissibletimeinterval(inminutes)betweentworeferencemeasurements.

Response A113ïA Timeintervalforreferencemeasurementconfiguredsuccessfully.



Command A113 Requestcurrenttimeintervalforreferencemeasurement.

Response A113ïAïTime Time:1...10 Currenttimeintervalinminutes


A114 – Switch the resolution of measured values(1/10d,seethenotesinsections5.3.3,5.4.2and9.3)

Command A114ïValue Value:0 1digitresolutionofmeasuredvalues(5decimalplaces)1 10digitsresolutionofmeasuredvalues(4decimalplaces).

Response A114ïA Resolutionofmeasuredvaluesconfiguredsuccessfully.

A114ïL Incorrectnumberofparametersorparametersoutsidethepermis-siblevaluerange(0or1).


Command A114 Requestcurrentresolutionofmeasuredvalues.

Response A114ïAïValue Value:0 Resolutionofmeasuredvaluesis1digit(5decimalplaces)1 Resolutionofmeasuredvaluesis10digits(4decimalplaces)



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A115 – Configure the vibration adapter(seesection4.4)

Command A115ïValue Value:1 Settingforextremelystableenvironments2 Settingforstableenvironments3 Settingforunstableenvironments.

Response A115ïA Vibrationadapterconfiguredsuccessfully.



Command A115 Requestcurrentsettingofthevibrationadapter.

Response A115ïAïValue Value:1 Settingforextremelystableenvironments2 Settingforstableenvironments3 Settingforunstableenvironments.


A116 – Configure the process adapter(seesection4.5)

Command A116ïValue Value:0 Processadapterswitchedoff(“OFF”),recommendedsettingfor MCP1 Notrecommended,mayaffectthemeasuringperformance!2 Notrecommended,mayaffectthemeasuringperformance!3 Notrecommended,mayaffectthemeasuringperformance!

Response A116ïA Processadapterconfiguredsuccessfully.



Command A116 Requestcurrentsettingoftheprocessadapter.

Response A116ïAïValue Value:0 Processadapterswitchedoff(“OFF”),recommendedsettingfor MCP1 Notrecommended,mayaffectthemeasuringperformance!2 Notrecommended,mayaffectthemeasuringperformance!3 Notrecommended,mayaffectthemeasuringperformance!



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A117 – Select the repeatability(seesection4.6)

Command A117ïValue Value:0 Functionswitchedoff,eachmeasurementvalueisconsidered stableandreleasedimmediately(“OFF”)1 Veryquickreleaseofthemeasurementvalue(“Std”)2 Quickreleaseofthemeasurementvalue(“Good”)3 Slowreleaseofthemeasurementvalue(“bEttEr”)4 Oncestabilityhasbeenachieved,themeasurementvalueis onlyreleasedifithasnotchangedforafewseconds (“bESt”).

Response A117ïA Repeatabilityconfiguredsuccessfully.



Command A117 Requestcurrentsettingoftherepeatability.

Response A117ïAïValue Value:0 Functionswitchedoff,eachmeasurementvalueisconsidered stableandreleasedimmediately(“OFF”)1 Veryquickreleaseofthemeasurementvalue(“Std”)2 Quickreleaseofthemeasurementvalue(“Good”)3 Slowreleaseofthemeasurementvalue(“bEttEr”)4 Oncestabilityhasbeenachieved,themeasurementvalueis onlyreleasedifithasnotchangedforafewseconds (“bESt”).


Recommendationsforpracticaluse

49

10 Recommendations for practical useWe recommend the following preventive maintenance work to keep the measuring performance of the MCP at top level:

Keeptheunitcleananddry.Distilledordeionizedwaterisveryaggressiveandcausessuperficialcorrosion(rustfilm)towetmetallicsurfacesevenifthesearemadeofhigh-qualitystainlesssteel.

10.1 Internal calibrationOnceaday,preferablyinthemorningbeforethefirstmeasurement,theMCPshouldbecalibratedinternally(pressandhold«Cal»key,seesection5.1).DonotdisconnecttheMCPfromthemainswhentheunitisnotused.WhentheunitwasdisconnectedfromthemainsorincaseofpowerfailuremakesuretolettheMCPwarmupforatleast60minutesbeforeperformingmeasurements.

10.2 Verification of the weighing cell calibrationAfteracertainperiodoftime(dependingtocustomer’sparticularrequirements)theMCP’sinternalcalibrationshouldbeverifiedwithanexternalweightusingtheoptionalcalibrationkit(orderinginformationisfoundinsection8.8).Theverificationintervaltobedefinedbythecustomerismeanttolimitpossibleinformationcampaignsorrecallactionsthatmaybemandatoryincaseofmeasurementerrors.Section11explainshowtousetheoptionalcalibrationkit.

MaximumadmissiblecalibrationtolerancesoftheMCPforpipettecalibrationaccordingtoISO8655:

Application tolerances measuredwith20 gF1orbetter(E2)

Tolerancefor0.03 %(alertlimit) 0.00700 g

Tolerancefor0.10 %(actionlimit) 0.02100 g

Thecalibrationtolerancevaluesmentionedaboveensurethatallpipettecalibrations(single-channelormultichannel)meettheISO8655requirementsevenincasethetolerancesspecifiedbythepipettemanufacturerareuptofivetimesmorerestrictive.

When will it be necessary to inform your clients in case of measurement errors?

Incasethe0.10%toleranceisexceeded,youshouldfirstverifytherealimpactonpipettecalibration.If,forexample,theadmissibletoleranceforbalancecalibrationerrorsisexceededbyafactorof10(i.e.1%),thisjustmeansthatthepipettecalibrationdatastatedinthecalibrationprotocolsaretoohighortoolowby1%.Dependingoncustomer’squalitystandardsthismayormaynotjustifyarecallaction.

10.3 Excess evaporationIfyouencounterexcessevaporationalthoughtheMCPisoperatedwellwithinthespecifiedambientconditions,thefollowingproblemmaybepresent:Ifthecontainersareoverfilledorhavebeeninclined,followingtransportationorafterthetubeshavebeenremoved,theremayberesidualwaterintheareawherethetubeentersthecontainer.Thisresidualliquidmaycausecapillaryactionthatmovesthewateroutofthecontainerwhichleadstoexcessevaporation.Inthiscase,removetheglasstubefromthecontainer(section7).UseKleenexorothersuitablecleaningpapertocarefullydrytheoutsideofthetubeaswellasthetubeguideintheupperpartofthecontainer,thenrelocatethetube.Preventoverfillingorincliningthecontainers.Checkthecriticalarea(wheretheglasstubeentersthecontainer)onaregularscheduleanddry,asrequired.

Workingwiththeoptionalcalibrationkit

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11 Working with the optional calibration kitTheoptionalcalibrationkit(orderinginformationseesection8.8) isusedtoverify the internalcalibrationof theMCP.Thissectiondescribestheinstallationanduseoftheoptionalcalibrationkit.Informationonthefrequencyofverificationsandthetolerancesarefoundinsection10.

11.1 Contents of the calibration kitThecalibrationkitissuppliedinaplasticcaseandincludesthefollowingitems:

1 Centeringring

2 Weighingpan

3 Draftshield

4 Coverglass

5 Calibrationweight20 ginprotectivebox(certificateincluded)

6 Tweezersinsleeve

11.2 Installation of the calibration kitSwitchtheMCPoffandremoveallassemblypartsformthemeasuringunit(containers,rack,weighingyoke,etc.).

Installthecenteringring.

1

2

3

4

5 6


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=O0000e

Mounttheweighingpan.

Installthedraftshieldandputonthecoverglass.

11.3 Carrying out the verification

Brieflypressthe«k»keytoresettheMCPdisplaytozero.

Liftoffthedraftshieldandcoverglass,thenusethetweezerstolocatethecalibrationweight(20 g)exactlyatthecenteroftheweighingpan.Relocatethedraftshieldandthecoverglass.


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Waituntilthedisplayreachesstability,thennotetheweightvaluedisplayed.

Removethecalibrationweight,thenperformaninternalcalibra-tion(section5.1).

Uponcompletionoftheinternalcalibration,resettheMCPdisplaytozeroandputonthecalibrationweightagain.Oncethedisplayreachesstability,theweightvaluemustbewithintheadmissibletolerances(applicationtolerancesseesection10,specificationsof theweighingmoduleseesection8.1). If thevalue isoutofeithertoleranceyoushouldcontacttheMETTLERTOLEDOservicedepartmentwithoutdelay.

1)99999e

CAL donE

CAL int

1)99996e

To protect your METTLER TOLEDO product’s future:METTLER TOLEDO Service assures the quality, measuring accuracy and preservation of value of all METTLER TOLEDO products for years to come.Please send for full details about our attractive terms of service. Thank you.

Subjecttotechnicalchangesandtotheavailabilityoftheaccessoriessuppliedwiththeinstruments.

©Mettler-ToledoAG200611780744BPrintedinSwitzerland0610/2.12

Mettler-Toledo AG, Laboratory & Weighing Technologies,CH-8606Greifensee,SwitzerlandPhone+41-1-9442211,Fax+41-1-9443060,Internet:http://www.mt.com

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