Neuron cell under strong laser beam
13
uron cell under strong laser be 江江江 2003.07.07 Living soft matter ?
-
Upload
nayda-mckinney -
Category
Documents
-
view
34 -
download
2
description
Neuron cell under strong laser beam. Living soft matter ?. 江宏仁 2003.07.07. NEURONAL MORPHOGENESIS AND STRUCTURAL PLASTICITY: Axon Initiation Axon Growth Axon Guidance Axon Branching Dendritic Growth, Guidance, and Branching Dendritic Spine Formation, Stability, and Rapid Motility - PowerPoint PPT Presentation
Transcript of Neuron cell under strong laser beam
Neuron cell under strong laser beam
江宏仁 2003.07.07
Living soft matter ?
NEURONAL MORPHOGENESIS ANDSTRUCTURAL PLASTICITY:
Axon Initiation Axon GrowthAxon Guidance Axon Branching Dendritic Growth, Guidance, and Branching Dendritic Spine Formation, Stability, and Rapid MotilitySynapse Formation and Stability Axon and Dendrite Retraction
PHENOMENOLOGY
Growrh cone
protrusion
anchorage
forward movement
tail retraction
4 processes coordinated:
Axon Guidance
Laser:1064nm 700mW source 50 mW after nosepiece
Guiding neuronal growth with light ?
Optical forces can impact diffusive behavior.
Theory 1
Mechanical manipulation of a membrane.
Theory 2
Dynamic Excitations in Membranes Induced by Optical Tweezers
Colloidal effects
Theory 3
PEARLING OF CYLINDRICAL VESICLES
Laser-induced rounding
Tests for each theory
Theory 1 :* in vitro actin polymeried under laser beam * Listeria movement as membrance moving model
Theory 2: *using flow cell to apply shear force on membrance
Theory 3: *using indirect phenomena ex: pearling, rounding, retrcation, or in water *direct observe by EM or light scattering
