MX3 HYDRATIONTESTING SYSTEMUSER MANUAL

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Table of Contents

Welcome 2

Intended Use 4

The MX3 Hydration Testing System 5

First Time Setup 10

Loading the MX3 Dispenser 11

Taking Measurements 12

Recommendations 15

Sample Collection Tips 17

Interpreting Measurements 18

Baseline Measurements/ Optimal Hydration Zone (OHZ) 19

FCC Compliance 22

Warranty 23

Technical Specifications 24

Medical Disclaimer 24

Contact Us 25

Welcome

Thank you for choosing MX3 Diagnostics!

This manual will guide you through the basics of how to use the MX3 Hydration Testing System (HTS) for measuring and tracking hydration status in teams, training groups and individual athletes.

The MX3 HTS is designed knowing our customers will benefit from performing laboratory-grade hydration testing anywhere, anytime.

Our system will transform how you think about hydration in order to optimize performance and improve overall health and well-being.

The MX3 Diagnostics Team

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Intended Use The MX3 Hydration Testing System

The MX3 HTS allows you to easily measure an athlete’s salivary osmolarity (SOSM) in seconds, anytime and anywhere, using a microliter of saliva. SOSM is a research-proven, non-invasive measure of hydration status and exercise-induced dehydration.

Hydration measurements are viewed in real time directly on the MX3 LAB or App and uploaded wirelessly to the MX3 Portal for a more detailed analysis. The MX3 App and Portal have been designed for use by professional teams and organizations of any size as well as individual athletes.

Continued use of the MX3 HTS will allow you to develop athlete-specific hydration strategies to optimize:

+ preparation + performance + recovery

The MX3 Hydration Testing System consists of six components:

1) MX3 LAB2) MX3 Hydration Test Strips3) MX3 Dispenser4) MX3 App 5) Charging Cable6) MX3 Portal (on personal device)

MX3 LAB

MX3 Dispenser

Charging Cable

MX3 Hydration Test Strips

MX3 App

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Test Strip

Test Strip Port

Display

Eject Button

Charging Port

LED Indicator

Select ButtonScroll Buttons

Power Button

Wrist Strap Eyelet

The MX3 LABThe MX3 Hydration Test Strip

The MX3 Hydration Test Strip is a single-use biosensor that samples saliva to determine the salivary osmolarity (SOSM) and hydration status of an athlete.

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The MX3 LAB is a powerful handheld device capable of laboratory-grade analysis. Simply insert a test strip, take a small saliva sample and get your actionable results in seconds.

This product complies with FCC Part 15.247 as a DTS device.

For further information see page 22.

The MX3 Test Strip Dispenser stores up to 45 sterilized test strips. It provides a clean, easy- to-use solution, minimizing potential contamination from manual handling.

The MX3 Test Strip Dispenser

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The MX3 App The MX3 Portal

The MX3 App, available on iOS and Android, tracks and analyzes hydration measurements. The App displays customized information for coaches, trainers and athletes, showing the most important information for each level of user.

The MX3 Portal is a centralized database of all hydration measurements collected by an organization. The portal can be used to add new teams, analyze measurement data and generate reports.

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First-Time Setup

Before using the MX3 Hydration Testing System, you must register and set up your MX3 Account.

A video demonstrating how to load the MX3 Dispenser can be found at mx3diagnostics.com/resources

Loading the MX3 Dispenser

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Open Dispenser

Open Test Strips Keep base tilted up to prevent strips from sliding out.

Insert Test Strips at Upward Angle

Remove Test Strip Holder

Push Tabs to Release Spring

Push Down Spring Until It Locks

Tilt Dispenser Up, Allowing Test Strips to Slide into Dispenser

Close Dispenser

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Pull Holder with Test Strips down from MX3 Cover

Test Strips may Fall!!!

Pull Holder with Test Strips down from MX3 Cover

Test Strips may Fall!!!

Pull Holder with Test Strips down from MX3 Cover

Test Strips may Fall!!!

Pull Holder with Test Strips down from MX3 Cover

Test Strips may Fall!!! Pull Holder with Test Strips down from MX3 Cover

Test Strips may Fall!!!

Pull Holder with Test Strips down from MX3 Cover

Test Strips may Fall!!!

Pull Holder with Test Strips down from MX3 Cover

Test Strips may Fall!!!

Account Registration

To sign up for an account visit portal.mx3diagnostics.com

MX3 offers three account types. Select one that best describes how you will use your MX3 Hydration Testing System.

App Access/User Registration

If you selected “Large organization/Professional Team” during account registration, you will need to set up teams, coaches, trainers and athletes in the MX3 Portal before use.

If you selected “Small Organization/Small Team” during account registration, you can now access the MX3 Portal or App using your username and password. You can set up athlete accounts using either the MX3 Portal or through the MX3 App.

If you selected “Individual” during account registration, you can now access the MX3 Portal or App using your username and password. You can also access the MX3 Portal to view and export past measurement data.

For information on how to set up user accounts and use the MX3 Portal, visit mx3diagnostics.com/resources

Download the MX3 App

To download the MX3 App, search for “MX3 Diagnostics” in the iOS App Store or Google Play Store.

Taking Measurements

Use the scroll buttons to highlight APP and press Select.

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Result will be displayed on both the LAB and the MX3 App.

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Ensure Bluetooth is enabled on your phone or tablet. Open the MX3 App and tap the icon or the Take a Measurement button to initiate a measurement.

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Follow the prompts to connect your MX3 LAB to the MX3 App. Insert a Hydration Test strip.

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Press the Eject Button to eject the test strip.

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Swallow saliva in mouth and generate fresh saliva.* Tap the tip of the test strip against the saliva sample at an approximately 30° angle until a tone sounds.

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*See Page 17 (Sample Collection Tips)

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The MX3 LAB may also be used in LOCAL mode to take measurements without using the App. LOCAL mode measurements are not recorded or stored in the App or the device itself, but allows readings to be made when a smartphone/tablet is unavailable. To take a measurement in LOCAL mode, turn on the MX3 LAB, scroll to highlight LOCAL, press Select and follow the process for taking a saliva sample. The reading will be displayed on the screen.

Local Measurements

For FAQs and Troubleshooting guides visit mx3diagnostics.com/resources

FAQs and Troubleshooting

MX3 technology combined with a hydration protocol will allow athletes to assess hydration, prepare for competition or training, perform at their best, and recover fully. Follow these recommendations to benefit most from MX3:

The MX3 Hydration Testing System (HTS) classifies hydration status into four possible categories: hydrated, mildly dehydrated, moderately dehydrated and severely dehydrated, by measuring salivary osmolarity (SOSM) based initially on populational hydration data from users of our system.

We recommend athletes use the baseline feature within the MX3 App to establish their personalized Optimal Hydration Zone (OHZ) (see page 19) corresponding to their fully hydrated state. By establishing an athlete’s OHZ, the MX3 App then customizes hydration status classifications based on their unique hydration profile.

MX3 Recommendations for Hydration Tracking

Assess

+ To maximize the use of the MX3 HTS, athletes should first establish their OHZ. Routine measurements will give each athlete deeper insight into their personal hydration fluctuations and highlight days when an athlete requires additional hydration.

Prepare

+ In preparation for competition or training, we recommend athletes measure their SOSM the day before training (or at least 4 hours before training/game) to ensure the athlete has enough time to hydrate if their SOSM score is higher than their OHZ.

+ Athletes who are consistently above their OHZ before training may be more susceptible to dehydration and therefore may require additional fluid intake during training and competition. They should also be encouraged to increase their fluid intake outside of training to improve preparation and recovery.

Perform

+ After training, athletes’ SOSM scores are likely to increase as they typically will not be able to offset all fluid losses that have occurred during training. When SOSM scores increase by more than 100% after exercise when compared to OHZ or pre-exercise measurement, fluid intake during training or competition has been insufficient.

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MX3 Hydration Testing System

Recommendations 15

Sample Collection Tips 17

Interpreting Measurements 18

Baseline Measurements / Optimal Hydration Zones (OHZ) 19

(continued on page 16)

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Sample Collection Tips

For a visual guide, see the instructional videos available at mx3diagnostics.com/resources

To optimize the accuracy of measurements taken with the MX3 System, we highly recommend the following:

+ Always have an athlete swallow all saliva and generate a fresh sample before each measurement.

+ Ensure an athlete did not eat or drink anything 5 minutes prior to taking a measurement.

+ Ensure a good amount of saliva is present on the athlete’s tongue. The tongue should be noticeably wet. If insufficient saliva is present for the sample, ask the athlete to swallow and generate a fresh sample. Insufficient saliva can result in an error.

+ Always avoid bubbly areas of saliva when collecting a sample. Collection of bubbles can result in an error or less accurate reading.

+ Do not cover the top of the test strip with saliva or a tongue. It is important to ensure saliva or any

part of the mouth does not cover the exit pore. The easiest way to ensure this is to sample with the LAB and test strip angled downwards.

+ Tapping on the saliva with the test strip will lead to a quicker measurement as it aids the uptake of saliva into the test strip.

+ If saliva sample is too viscous, we recommend using the MX3 sample tray to collect saliva and taking a measurement from there. This will facilitate making the measurement. If still difficult, ask the athlete to drink a small amount of water, wait 5 minutes and remeasure.

Exit Pore (DO NOT SUBMERGE OR COVER)

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+ When an athlete consistently increases their SOSM by more than 100% post-exercise, encourage the athlete to actively increase their fluid intake in subsequent training sessions.

Recover

+ In the hour(s) and or days following competition and training sessions, athletes should rehydrate and monitor their SOSM.

+ Athletes should be re-tested periodically until their SOSM scores return to within or close to their OHZ/Pre-Training Score (± 15mOsm). Where an athlete regularly does not achieve full recovery of their SOSM several hours after training or by the following day, advise the athlete to increase fluid rehydration during recovery.

• For athletes who consistently do not recover their SOSM in a reasonable amount of time, consider utilizing fluids for recovery that contain additional electrolytes and/or carbohydrates to boost speed of rehydration and recovery.

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In basic terms, SOSM values increase as dehydration increases, and they decrease as hydration status improves. Most athletes will exhibit a gradual increase in SOSM as they exercise.

While comparison of SOSM measurements with population averages can provide an estimate of hydration status, every athlete’s SOSM range and hydration response is unique. To provide the most accurate hydration analysis, SOSM measurements should be compared against an optimal hydration zone determined by Baseline measurements (see page 19).

The interpretation of measurements is dependent on the context in which they were taken.

Interpreting Measurements

+ When measuring at rest or immediately before exercise, a dehydrated measurement suggests an athlete is not regularly drinking enough fluid to be fully hydrated and should increase daily fluid intake.

+ If measuring immediately after exercise, a severely dehydrated score suggests that fluid intake during exercise has been insufficient to avoid significant dehydration. In this scenario, an athlete should continue to hydrate with fluids and monitor SOSM over a period of hours until an athlete’s

SOSM reading returns to within 15mOsm of an athlete’s optimal hydration zone (OHZ).

+ When measuring in the hours following exercise, dehydrated readings suggest that additional fluid intake is required to recover from fluid losses during exercise. Fluid intake should continue (at a sensible rate) until SOSM measurements are within 15mOsm of an athelete’s OHZ.

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Baseline Measurements / Optimal Hydration Zone

The MX3 HTS initially classifies hydration status using populational data.

While comparison of SOSM measurements with population averages can provide an estimate of hydration status, every athlete’s SOSM range and hydration response is unique. To provide the most accurate hydration analysis, SOSM measurements should be compared against an optimal hydration zone (OHZ) determined by carrying out Baseline measurements.

An athlete’s OHZ is also displayed on the results bar after taking a hydration measurement. This allows an athlete to PREPARE for training or a game by targeting their personal OHZ or to RECOVER after the game by rehydrating and assessing hydration status until their SOSM returns to a value that is close to or within their OHZ.

To add a measurement to an athlete’s baseline, click the ADD TO BASELINE Button on the measurement result page. A minimum of 6 baseline measurements is needed to calculate an OHZ.

Prior to taking a baseline measurement, we recommend following a hydration protocol to ensure an athlete is well hydrated. A recommended example protocol can be found on page 20.

If following our suggested hydration protocol is not feasible, an athlete or trainer may choose instead to define readings as baseline measures when they feel water consumption has been adequate to achieve a fully hydrated state.

Example Baseline Protocol Notes

Day 1

+ Consume 1 standard glass of water (250 ml) every hour from 9/10am to 5/6pm in addition to any water typically consumed during or after exercise.

+ Limit alcohol consumption (1 standard drink) to avoid alcohol-related dehydration affecting

baseline readings.

Day 2

+ Consume 1 standard glass of water (250 ml) every 45mins from 9/10am to 5/6pm in addition to any water typically consumed during or after exercise. We recommend conducting baseline measures on days where exercise is light to minimize exercise-related dehydration increasing baseline readings.

+ Avoid eating or drinking 15 minutes before taking a baseline measurement.

+ Take 3 baseline readings at 12pm and 3 baseline readings at 5/6pm.

+ We recommend not to consume any alcohol during day 2 to avoid

alcohol-related dehydration effects.

Day 3+

+ For greater precision, repeat the process outlined on day 2 for

additional days, up to 3 days total.

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The MX3 Hydration Testing System comes with a 1-year limited warranty against defects in materials and workmanship from the date of delivery. A 90-day extended warranty applies to repairs returned within the last 90 days of the warranty period. The rights outlined here are in addition to your statutory rights provided by Australian or US consumer law.

Eligible goods may be replaced with a new or refurbished equivalent or superior product (where applicable). Eligible goods may also be repaired with new or refurbished parts or refunded at MX3’s discretion.

This warranty will not apply to:

+ Cosmetic damage due to regular use

+ Damage caused by unintended use

+ Damage caused by external causes

+ Damage caused by non-authorized disassembly, servicing or modification.

This warranty does not guarantee uninterrupted or error-free use. MX3 is not liable for any

incidental damage resulting from defects (as limited by law).

To claim the warranty, you must contact MX3 support at support@mx3diagnostics.com within the warranty period with proof-of-purchase. If online support or in-person service is not available, you may be required to mail-in your Hydration Testing System for service. For such situations, MX3 will cover shipping to and from your location.

For Australian Customers: Our goods come with guarantees that cannot be excluded under the Australian Consumer Law. You are entitled to a replacement or refund for a major failure and compensation for any other reasonably foreseeable loss or damage. You are also entitled to have the goods repaired or replaced if the goods fail to be of acceptable quality and the failure does not amount to a major failure.

Warranty Information

Warranty Obligor Information

Name: MX3 Diagnostics Inc.

Business Address: MX3 Diagnostics, Inc. c/o WeWork 801 Barton Springs Rd. Austin, TX 78704

Email Address: support@mx3diagnostics.com

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FCC Compliance

This product complies with FCC part 15.247 as a DTS device.

This Device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions:

(1)This device may not cause harmful interference, and

(2) This device must accept any interference received including interference that may cause undesired operation.

NOTE: THE GRANTEE IS NOT RESPONSIBLE FOR ANY CHANGES OR MODIFICATIONS NOT EXPRESSLY APPROVED BY THE PARTY RESPONSIBLE FOR COMPLIANCE. SUCH MODIFICATIONS COULD VOID THE USER’S AUTHORITY TO OPERATE THE EQUIPMENT.

To access the FCC information on the MX3 LAB, Select INFO on the main menu. The following information will be displayed:

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Contact Us

For further questions about the MX3 System please contact us:

MX3 Diagnostics, Inc. c/o WeWork801 Barton Springs RoadAustin, TX 78704 USA

MX3 Diagnostics Pty. Ltd. Suite 43, 255 Drummond Street,Carlton VIC 3053Australia

For sales inquiries: sales@mx3diagnostics.comFor technical support: support@mx3diagnostics.com

Website: www.mx3diagnostics.com

MX3 LAB Technical Specifications

Medical Disclaimer

+ Size 8.4 x 1.8 x 1.0 in / 214 x 45 x 25 mm

+ Weight 5.6 oz / 160 g

+ FCC Compliance 15.107, 15.109 Class A

+ Bluetooth version 5.1

+ Battery life more than 1000 measurements when fully charged

+ Battery type Single Cell rechargeable, Li-Po, 3.7V, 1100mAh

+ Charging port USB-C

The MX3 hydration score or hydration category is not medical advice. If you or your athletes are experiencing headaches, dizziness, nausea or other symptoms of dehydration after exercise, please seek immediate medical attention.

Hydration categories and advice are based off population data and may vary from your personal hydration profile calculated from a user’s OHZ.

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