Multispecies Cytograms
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Transcript of Multispecies Cytograms
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Cat WBCThis area includes nonlysed MNs that are included in the MN count. Samples should be run at room temp to minimize nonlysis.Eoss average 2%-12% but may be as high as 35%Eoss not counted hereEoss are counted in the retic channel (even without ordering a retic). Retic reagent must be available on the analyzer at all times when running cats.
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Cat with Large Platelets
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Cat with Platelet ClumpsPlatelet clumps appear herePlatelet clump count located here
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Cat HGB, Platelet and RBCHigh MPV (cat MPV value can be up to 25)Platelets can be larger than RBCsPlatelet cytogram is very diffuse due to platelet activation.
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Cat ReticEoss are counted in the retic channel. Three times as much sample is pushed through the flowcell when running cat. Throughput for CBC/Diff on cat is 74 samples/hour.Low Retic count normal for cat
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Cattle WBCElongated MN population
Cattle can have 0-25% EOSsMonoLUC
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Cattle HGB, Platelet, and RBCLow MCV
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Cattle ReticCattle have a very low retic count or no retics at all therefore retics do not need to be run on cattle.
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Dog WBCBaso positioned lower
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Dog HGB, RBC and PlateletPlatelets look very diffuse - activatedLarge platelets commonMPV can be 15LPlatelet activation may be a function of the draw technique
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Dog Platelet (with less platelet activation)Platelets less diffuse
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Dog Retic%Retic low on dogs (but can be 5%)
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Goat WBC
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Goat HGB, Platelet and RBCGoats can have 1,000,000 plateletsRBC count very high and MCV very low2D platelet analysis used to obtain RBC countLower threshold set at zero
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Goat ReticLow retic resultsNo Retic parameters
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Guinea Pig WBC
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Guinea Pig HGB, Platelet and RBCSmall Platelets
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Guinea Pig Retic
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Horse WBCEoss are located out to the side and are giant in sizeMonosLUCsUnlysed MN - can warm the blood but non-lysis may not be eliminated
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Horse HGB, Platelet and RBCLow MCV
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Horse ReticHorses do not have retics
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Monkey-Cyno WBCPerox cytogram looks like a weak staining human
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Monkey-Cyno HGB, Platelet and RBC
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Monkey-Cyno Retic
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Monkey-Rhesus WBC
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Monkey-Rhesus HGB, Platelet and RBC
- Monkey-Rhesus Retic
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Mouse WBCMice do not have many monosInverted DiffLipids common for mice
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Mouse with Platelet ClumpsPlatelet clumps
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Mouse HGB, Platelet and RBCRBCMCVPlateletMPVPlatelet count about 5% higher (on samples with platelet clumps) than H1E due to difference in mixing between analyzers
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Mouse ReticCommon pattern
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Pig WBC
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Pig HGB, Platelet and RBC
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Pig Retic
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Rabbit WBCMany BasosVery little noiseLymph threshold is low due to small size of lymphsBasos can equal 4-10%
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Rabbit HGB, Platelet and RBCSmall Platelets
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Rabbit ReticRabbits can have 5%-6% Retics
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Rat WBCInverted diffPlatelet clumps common (see next Run Screen)
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Rat-LE with Platelet ClumpsPlatelet Clumps common due to draw technique
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Rat-LE HGB, Platelet and RBCPlatelet count usually higher on the ADVIA 120 (for samples with platelet clumps than H1E due to differences in mixing between analyzers Platelet Clumps common
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Rat ReticRat Retic >2%
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Sheep WBCLUCsMonosLymphsInverted Diff
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Sheep HGB, Platelet and RBCVolume Threshold set to zeroRBCMCV
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Sheep ReticLow %Retic