Mouse Models of Prostate Cancer Name: Vicki Javier Mentor: Todd Hricik Institution: MSKCC.
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Transcript of Mouse Models of Prostate Cancer Name: Vicki Javier Mentor: Todd Hricik Institution: MSKCC.
![Page 1: Mouse Models of Prostate Cancer Name: Vicki Javier Mentor: Todd Hricik Institution: MSKCC.](https://reader034.fdocuments.us/reader034/viewer/2022051516/56649ee65503460f94bf5fa4/html5/thumbnails/1.jpg)
Mouse Models of Prostate Cancer
Name: Vicki Javier
Mentor: Todd Hricik
Institution: MSKCC
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• Prostate Cancer: disease marked by abnormal growth of tumor cells in the prostate gland
• Prostate Cancer is detected by– Digital Rectal Exam– Biopsy– PSA serum reading
Prostate Cancer
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• PSA is a biomarker for CaP
• Our Question: Does PSA play a role in CaP?-Mouse studies
PSA Serum Screen
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PSA mouse study
• Mice don’t have PSA
• We already put WT PSA gene into mice
• We now need a mutant form of PSA gene to put in mice so that we can compare whether the mut mouse will develop CaP in a different way than the WT PSA mouse
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• Mutate the PSA gene (in a plasmid) nucleotide sequence so that a Serine amino acid is changed to an Alanine in the PSA protein (gene product)
• Prepare the mutated PSA gene for injection into the mouse
Project Goal
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Promoter WT PSA Ser 189 SV40 Poly A
NotIKpnI
EcoRI
EcoRI
Promoter Mut PSA Ser 189 to Ala SV40 Poly A
NotIKpnI
EcoRI
EcoRI
SDM
Site Directed Mutagenesis
TCT = Serine
GCT = Alanine
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![Page 8: Mouse Models of Prostate Cancer Name: Vicki Javier Mentor: Todd Hricik Institution: MSKCC.](https://reader034.fdocuments.us/reader034/viewer/2022051516/56649ee65503460f94bf5fa4/html5/thumbnails/8.jpg)
Tools
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![Page 10: Mouse Models of Prostate Cancer Name: Vicki Javier Mentor: Todd Hricik Institution: MSKCC.](https://reader034.fdocuments.us/reader034/viewer/2022051516/56649ee65503460f94bf5fa4/html5/thumbnails/10.jpg)
Restriction Digestion of DNA
Direction of DNA travel
6000 bp5000 bp
250 bp
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References
• Hricik, Todd
• http://www.web-books.com/MoBio/Free/Ch9G.htm
• http://en.wikipedia.org/wiki/Site-directed_mutagenesis
• http://www.ehealthmd.com/library/prostateremoval/PR_glossary.html
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Acknowledgements
• Todd Hricik
• Dr. Sat Bhattacharya
• Sven
• Ross
• Neil