Mosaic Digital Illumination - Harvard...
Transcript of Mosaic Digital Illumination - Harvard...
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Mosaic Digital Illumination
Specifications Summary
Transmission 360 nm to 800 nm
Extinction ratio > 1000:1
Minimum resolvable spot Diffraction limited with 60x or 100x objective
Optical pixel rise / fall time < 1 µs
Minimum optical pulse width75 µs external trigger100 µs internal trigger
Maximum frame repition rate 600 frames / sec
Certification CDRH IIIb (if fitted with a laser source)
Features and Benefits • Unlimited flexibility in shape, size and
complexity of illumination mask
• Simultaneous illumination of multiple
regions of interest
• Precise illumination of areas of interest
that protects target specimen and
fluorophore
• Zero delta acquisition time for true
digital excitation/illumination
• Longest lifetime and lowest
maintenance with rugged
semiconductor device
Mosaic Real Time Parallel Illumination of Multiple RegionsMosaic is a patented instrument platform built around MEMS Digital Mirror Devices (DMD). Mosaic
exploits DMD in a proprietary programmable platform, integrated with scientific light sources
including lasers, LEDs and arc lamps, and operates from 360 to 800 nm. It is offered with a range
of high performance microscope adapter optics and can be integrated with CLSM, spinning disk
and wide field imaging modalities. High speed frame switching (60 Hz) makes Mosaic suitable for
many dynamic applications including bleaching, uncaging, photo-switching, optogenetics and
constrained illumination. Variable intensity distributions can be achieved by rapid gating of mirror
patterns.
DMD TechnologyThe core of Mosaic is the Digital
Micromirror Device (DMD), a high speed
and highly efficient semiconductor-based “light
switch” array of hundreds of thousands
hinge-mounted, addressable, tiltable,
microscopic mirrors.
When a DMD chip is coordinated with a digital
video or graphic signal, a light source, and
beam delivery optics, its mirrors reflect a
digital image of the illumination mask onto the
sample.
Active Illum
ination
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Mosaic Digital Illumination
Illumination Source Options
DMD illumination ports 1 or 2 on left hand side* 1 or 2 on left hand side, 1 or 2 on right hand side*
Incoherent sources High pressure mercury lamp (100 W - 300W)DG4
Andor AMH-200Other light sources on request
CW laser 405 nm diode488 nm Argon514 nm Argon
Other on request
* For 7201 / 7204 / 7205 allow only 1 port, 7206 allows 2 portsAdditional illumination source options available on request
Hardware SpecificationsOptical
Model Mosaic Mosaic Duet
Transmission 360 nm to 800 nm
Intensity stability Absolute
Extinction ratio > 1000:1
Minimum resolvable spot Diffraction limited with 100x objective
Fields of view 7206 = 5.28 x 3.96 mm 7201 = 8.64 x 6.48 mm
7204 and 7205 = 13.12 x 9.84 mm
Illumination port clear aperture Ø 34 mm
For flat field illumination port options, please see table at bottom of this secion
Illumination Port Options
Dichroic beamsplitter Single pass, specify wavelengthMulti-pass, specify wavelength
Beamsplitter 360 nm to 700 nm, R = 100, 70, 50 or 30%
Excitation filter * 480 nm / 20 nm (GFP), 470 nm / 40 nm (FITC) - 38 mmOther filters on request - 25 mm with adapter
Laser safety filter, interlocks Laser specific
Shutter Optional
* Additional excitation filter options available on request
INTEROPERABILITYCompatible with microscopes platforms - point & slit scan confocal, spinning disk confocal & widefield.
Custom OEM systems available for High Content Screening and other fluorescence based imaging instrumentation.
Compatible with control and acquisition software from market leading microscope manufacturers including Carl Zeiss,
Leica and Olympus.
Wavelength modularity - add lasers and filters cost effectively.
Flat field Epi illumination ports
Adapter Leica DMI series Nikon TE2000 Nikon Ti series Nikon Universal Olympus IX series
Part Number MM-7310-DMI-FFEP-X* MM-7310-TE2K-FFEP-X* MM-7310-TI-FFEP-X* MM-7310-NFS-FFEP-X* MM-7310-IX70-FFEP-X*
Adapter Zeiss Axiovert 200 Zeiss Axio Examiner D1 Zeiss Axio Imager M1 Zeiss Axio Observer C-mount video port
Part Number MM-7310-ZSAV-FFEP-X* MM-7310-D1-FFEP-X* MM-7310-IMG-FFEP-X* MM-7310-OBS-FFEP-X* On request
*For 7201 / 7204 / 7205, X = 1*For 7206, X = 2
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Mosaic Digital Illumination
Software SpecificationsThe Mosaic system includes software that controls calibration and enables the user to graphically or numerically define and edit the shape and
characteristics of the illumination at the target.
Release MicroPoint Mosaic Version 1.1
Drivers PLX 9054 API version 4.1 for Mosaic.exe UIPLX 9054 API version 6 for SDK
Calibration Scale & Offset corrections for X & Y8 user-defined descriptions
Operating systems Windows 2000, XP
File Functions Import (*.dxf, *.bmp, *.msk); Save *.msk
Drawing Editor Shapes Line, Paintbrush, Rectangle, Oval, Freehand polyshape
Drawn Shape Characteristics Linewidth, paintbrush size, Alpha blended color and opacity of defined regions, Color palletteClick and drag shapes and edges, ut, copy, paste, delete shape, Group, ungroup shapes
Controls Engage mask pattern, Go dark (global), Stop, Snapshot video
Options Continuous update from drawing editor, Configure video subsystem, Exposure time & trigger selectionVideo mode & time selection, On/Off, Adjust laser outline highlight
The angular alignment of either the CW laser or lamp illumination is factory set. The spatial alignment is controlled via the software calibration routine. The
focus in the z direction is fixed at installation or OEM integration. Beam shaping is then controlled with the spatial light modulating DMD.
Define region of interest and excitation
energy at the target in image acquisition
software
Simultaneously excite and acquire image
with zero delta time
Software Compatibility - Life Science ApplicationsMosaic systems are compatible with a wide range of life science imaging software as indicated in the table below.
Andor iQ Version 2.0 Live cell multi-dimensional imaging with fexible control of MicropPoint
MicroPoint Laser Galvo Version 1.0 Image-based targeting, control & calibration
MDC MetaMorph Ver 7.1 and above (32-bit) Fully integrated MicroPoint support with powerful imaging capabilities
MDC MetaMorph Ver 7.74 and above (64-bit) Fully integrated MicroPoint support with powerful imaging capabilities
Nikon NIS Elements 3.0 SP4 Build 505 Multi-dimensional imaging with simple/fast control of MicroPoint
Software Development Kits (SDK) SDKs are available for OEM to develop software to control integrated MicroPoint systems
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Mosaic Digital Illumination
ComponentsThe main components of MicroPoint are shown below:
Flange for EPI source
DMD array
Flange for optional second light source
Beamsplitter/ dichroic plugs
Laser zoom collimatorLaser fiber input
Electronic interface
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Mosaic Digital Illumination
Ordering your Mosaic system
Prior to commencing the order process please advise your customer representative of your application requirements. Please also refer to the flow charts on the next 2 pages.
In order to allow us to select the correct model for your requirements please advise your customer representative of the make, model (and any accessories fitted) of the microscope being used.
Step 1.
Please select the field of illumination required.
Step 2.
Choose one or more EPI / Mosaic beamspliiters.
Step 6.
Select the type of EPI light source require.
Step 4.
Select the primary left sided DMD light source. For the 7206 option only, a secondary left side light source can also be selected at this point.
Step 3a.
For Mosaic Duet systems only a primary right sided light source can also be selected. For the 7206 option only, a secondary right side light source can also be selected at this point
Step 3b.
Step 1.
Confirm your Microscope make and modelLeica, Nikon, Olympus, Zeiss
Step 2.
The following field of ilumination options (diagonal) are available:
7201 11.5 mm; 7204 17.4 mm; 7205 17 mm Axio Tirf; 7206 7 mm
Choose one or more optional EPI excitation filters.
Step 5.
Step 5.
Optional EPI Excitation filters are also available:
GFP filter in holder (480/20), FITC (470/40), Others on request
Step 4.
The following EPI light sources are available:
Andor AMH, DG4, Microscope specific Hg lamp, MicroPoint, other
Step 3a.
The following DMD light sources / filter sets for the left side port of Mosaic are available:
Diode laser, Andor ALC, Other laser, Andor AMH, DG4, Hg
The following left sided excitation filters are available:
GFP filter in holder (480/20), FITC (470/40) ,Blank filter holder, Others on request
Secondary left sided DMD source (7206 only), select one or more of the following
beamsplitters:
30 Epi, 50 Epi,70 Epi, 100% Epi Plug; 0% Epi (Blank) Plug, A range of dichroic mirrors is
available on request.
Step 6.
The following Epi-Mosaic beamsplitters are available:
30 EPI, 50 EPi, 70 EPI, 100% Epi plug, 0% Epi (Blank) Plug
Step 3b.
For Mosaic Duet systems only, additional DMD ight sources / filter sets for the right side port:
Diode laser, Andor ALC, Other laser, Andor AMH, DG4, Hg
The following right sided excitation filters are available:
GFP filter in holder (480/20), FITC (470/40) ,Blank filter holder, Others on request
Secondary right sided DMD source (7206 only), select one or more of the following
beamsplitters:
30 Epi, 50 Epi,70 Epi, 100% Epi Plug; 0% Epi (Blank) Plug, A range of dichroic mirrors is
available on request.
Mos
aic
Dig
ital I
llum
inat
ion
Pag
e 6
of 9
ww
w.a
ndor
.com
dis
cove
r ne
w w
ays
of s
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Mul
tiple
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ith M
icro
Poi
ntFo
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equi
ring
bot
h ab
latio
n an
d z
ero
del
ta t
ime
fluor
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nce
exci
tatio
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mul
tiple
reg
ions
with
com
ple
x ge
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the
Mos
aic’
s E
pi I
llum
inat
ion
por
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ted
with
a M
icro
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yste
m. a
nd fi
lterin
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on
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osai
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put s
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elay
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saic
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Sec
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6 on
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Exc
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Filt
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GFP
�lte
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hol
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(480
x40
nm)
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lank
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lder
30 E
pi :
70 L
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Bea
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litte
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lug
50 E
pi :
50 L
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Bea
msp
litte
r P
lug
70 E
pi:
30 L
aser
Bea
msp
litte
r P
lug
100%
Ep
i Plu
g0%
Ep
i (B
lank
) Plu
g
Op
tio
nal
Ep
i Exc
itat
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Filt
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(Cho
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one
or m
ore
if re
qui
red
)5
FITC
exc
iter
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FP e
xcite
r �l
ter
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dam
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ter
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lder
Fiel
d o
f Illu
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n(C
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EP
I / M
osa
ic B
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split
ter
(Cho
ose
one)
630
Epi
:70
Lase
r B
eam
split
ter
Plu
g50
Epi
:50
Lase
r B
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split
ter
Plu
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Epi
: 30
Lase
r B
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split
ter
Plu
g
100%
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lug
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i Lig
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roP
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HD
G4
Hg
Oth
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Con
figur
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Mos
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The
Mos
aic
can
be
tailo
red
to
suit
your
nee
ds,
the
re a
re a
num
ber
of o
ptio
ns in
clud
ing
mos
aic
illum
inat
ion
sour
ces,
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i illu
min
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n so
urce
s, A
ctiv
e m
irror
arr
ay
mag
nific
atio
n an
d fi
lterin
g.
Mos
aic
Dig
ital I
llum
inat
ion
Pag
e 7
of 9
ww
w.a
ndor
.com
dis
cove
r ne
w w
ays
of s
eein
g ™
11.5
mm
17.4
mm
17 m
m A
xio
TIR
F2
7.1m
mFi
eld
of I
llum
inat
ion
(Cho
ose
one)
Mic
rosc
op
e O
pti
on
sM
osai
c illu
min
atio
n in
put s
hutt
er w
ith c
ontr
olle
r &
BN
C c
able
Lase
r de
liver
y /
safe
ty �
lters
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e C
hann
el T
TL s
ynch
roni
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n m
odul
e w
ith d
elay
and
dura
tion
inpu
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mar
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eft
Sid
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igh
t S
ou
rce
(Cho
ose
one)
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icro
sco
pe
N.B
. If
lase
r ch
osen
, the
n la
ser
kit (
cont
aini
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uore
scen
t ta
rget
, �b
er c
oup
ler,
hom
ogen
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, �b
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& in
terlo
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de
lase
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MH
DG
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i / M
osa
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eam
split
ter
(Cho
ose
one)
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:70
Lase
r B
eam
split
ter
Plu
g50
Epi
:50
Lase
r B
eam
split
ter
Plu
g70
Epi
: 30
Lase
r B
eam
split
ter
Plu
g
100%
Epi
Plu
g0%
Epi
(Bla
nk) P
lug
Op
tio
nal
Ep
i Exc
itat
ion
Filt
ers
(Cho
ose
one
or m
ore
if re
qui
red
)5
FITC
exc
iter
�lte
rG
FP e
xcite
r �l
ter
Rho
dam
ine
exci
ter
�lte
rB
lank
�lte
r ho
lder
Bea
msp
litte
rs(C
hoos
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mor
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206
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Sec
on
dar
y L
eft
Sid
eL
igh
t S
ou
rce
(720
6 on
ly)
Lef
t S
ide
Exc
itat
ion
Filt
er(C
hoos
e on
e)La
rge
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�lte
r in
hol
der
(480
x40
nm)
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e b
lank
�lte
r ho
lder
30 E
pi :
70 L
aser
Bea
msp
litte
r P
lug
50 E
pi :
50 L
aser
Bea
msp
litte
r P
lug
70 E
pi:
30 L
aser
Bea
msp
litte
r P
lug
100%
Ep
i Plu
g0%
Ep
i (B
lank
) Plu
g
Ep
i Lig
ht
So
urc
e(C
hoos
e on
e)4
Mic
roP
oint
AM
HD
G4
Hg
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er
Pri
mar
y R
igh
t S
ided
Lig
ht
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urc
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.B.
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col
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req
uire
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Bea
msp
litte
rs(C
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e or
mor
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206
only
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Sec
on
dar
y R
igh
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ide
Lig
ht
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206
only
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bla
nk �
lter
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msp
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50 E
pi :
50 L
aser
Bea
msp
litte
r P
lug
70 E
pi:
30 L
aser
Bea
msp
litte
r P
lug
100%
Ep
i Plu
g0%
Ep
i (B
lank
) Plu
g
Dio
de
lase
rA
LCA
MH
DG
4H
gO
ther
Oly
mp
usN
iko
nZ
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Leic
a
Con
figur
ing
Mos
aic
Due
t (C
ompl
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tary
Mul
ticol
our I
llum
inat
ion)
Som
e p
hoto
sens
itive
pro
tein
s ca
n b
e ac
tivat
ed w
ith o
ne w
avel
engt
h of
ligh
t an
d d
eact
ivat
ed o
r “s
ilenc
ed”
with
ano
ther
. Thi
s p
rop
erty
is e
xplo
ited
in t
he M
osai
c D
uet
e.g.
to s
harp
en c
ontr
ol o
ver
pro
tein
loca
lizat
ion.
A s
econ
d li
ght
sour
ce is
cou
ple
d in
to t
he D
MD
at
a co
mp
lem
enta
ry a
ngle
to
the
prim
ary.
In t
his
confi
gura
tion
pix
els
NO
T se
lect
ed fo
r ac
tivat
ion
defi
ne a
n in
vers
e m
ask
whi
ch c
an b
e ill
umin
ated
to
“sile
nce”
mol
ecul
es o
utsi
de
of t
he a
ctiv
atio
n re
gion
(s) o
r th
ose
diff
usin
g aw
ay.
Page 8 of 9www.andor.com discover new ways of seeing™
Mosaic Digital Illumination
0
Shutter Moves 38.1[1.50]
Removeable Beamsplitter57 [2.2] Ø Cylinder
Duet
76
.2 [
3.0
]
Shutter Moves 38.1[1.50]
80 [3.1]
0
(Laser Input Attachment)
160 [6.3]240 [9.375]
(Lamphouse Attachment Plane)
Mosaic Lamphouse Adapter
Duet
76
.2 [
3.0
]
160 [6.3]
333 [13.1]
229 [9.0]
146 [5.8]+ 100 [4] Fiber Bend Radius
Laser Fiber Collimator
Shutter
Duet
Shutter
Weights:
Mosaic Head = 3.5 kg [7.5 lb]
Mosiac Duet Head = 4.5 kg [9.8 lb]
Product DrawingsDimensions in mm [inches]
7206
7201 - 7205
Head Dimensions
Model Part Number Length
7201 MEC-04686 369.06 mm [14.53]
7204 MEC-04685 385.57 mm [15.18]
7205 MEC-04684 356.06 mm [14.03]
7206 MEC-04729 333.00 mm [13.1]
Fields of Illumination (FoI)
Model 7201 7204 7205 7206
FOV (Lamp house)Diagonal, mm 11.54 17.44 17.44 7.12
FOV (Lamp house)XY, mm 9.23 x 6.92 13.95 x 10.46 13.95 x 10.46 4.88 x 3.66
FOV 0.4 mm fiber ZC* x2 (mm)** 8.40 X 6.92 11.90 X 10.46 11.90 X 10.46 4.2 X 3.66
FOV 0.4 mm fiber ZC x1 (mm)*** 4.4 6.7 6.7 2.7
FOV 0.2 mm fiber ZC x2 (mm)** 4.0 6.1 6.1 2.5
FOV 0.2 mm fiber ZC x2 (mm)** 2.2 3.4 3.4 1.4
DMD pixel size (µm) 12 17 17 6
*ZC = Zoom Collimator X2 = max; X1 = min ** Intersection of circle and DMD rectangle*** Circular field of illumination
Shutter
Duet
0
Shutter Moves 38.1[1.50]
Removeable Beamsplitter57 [2.2] Ø Cylinder
Duet
76
.2 [
3.0
]
Shutter Moves 38.1[1.50]
See Table
14
6 [
5.8
]+
100
[4]
Fib
er B
end
Rad
ius
80 [3.1]
0
97 [3.8]
Duet
76
.2 [
3.0
]
97 [3.8]
175 [6.88](Lamphouse Attachment Plane)
Mosaic Lamphouse Adapter
Laser Fiber Collimator
Shutter
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Mosaic Digital Illumination
Order TodayNeed more information? At Andor we are committed to finding the correct solution for you. With a dedicated team of technical advisors, we are able to offer you one-to-one guidance and technical support on all Andor products. For a full listing of our regional sales offices, please see:
Our regional headquarters are:Europe Japan
Belfast, Northern Ireland Tokyo
Phone +44 (28) 9023 7126 Phone +81 (3) 3518 6488
Fax +44 (28) 9031 0792 Fax +81 (3) 3518 6489
North America China
Connecticut, USA Beijing
Phone +1 (860) 290 9211 Phone +86 (10) 5129 4977
Fax +1 (860) 290 9566 Fax +86 (10) 6445 5401
www.andor.com/contact
MMosaicSS 0411 R2
Minimum Computer Requirements:
The imaging computer must have two adjacent PCI
slots (with exceptions) and a bay for BNC Trigger
connectors. Application software for the Mosaic
Digital Diaphragm System is typically integrated into
the imaging software provided for the microscope.
Operating & Storage Conditions
Operating Temperature 0°C to 30°C ambient
Relative Humidity < 70% (non-condensing)
Storage Temperature -25°C to 50°C
NOTE: Laser radiation is only present in Mosaic systems fitted with a laser. Some Mosaic systems use non laser-based light sources.
1x DMD Head assembly, Mosaic II
1x PLX and daughter board pair, programmed
1x 10’ SCSI-3 Ultra LVD/SE MD698 cable
1x TPES15-T050PP-7 Mosaic custom power supply
1x 3m mains cable for TPES15-T050PP-7
1x First surface mirror
10x Green Post-It notes
1x Allen key (0.05”)
1x T-handle (3/32)
1x LED Maglite, with 2 xAA batteries
1x Maglite clamp
1x Mosaic post holder
1x Mosaic foot
1x NT-59-000 4” stainless steel post (1/4 - 20 UNC)
1x NT-58-999 2” stainless steel post (1/4 - 20 UNC)
1x Zeiss male flange
1x BNC finger assembly
1x User guide
Items shipped with your Mosaic:
Windows is a registered trademark of Microsoft Corporation.Power Requirements
100 - 240 VAC, 50-60 Hz, 1.0 A