Molecular Metabolism A PhD program to Learn and Explore in ... · programm 420 2014-2017 FWF W1226...

Molecular Metabolism –

A PhD program to Learn and Explore in the

Life Sciences (MOBILES)

Curriculum Vitae and Publication List of Faculty Members

E1-Ronald P. Kühnlein………………………………………………………………………1

E2-Frank Madeo……………………………………………………………………………..6

E3-Monika Oberer………………………………………………………………………….16

E4-Brigitte Pertschy……………………………………………………………………..….22

E5-Stefan Schild……………………………………………………………………………27

E6-Ulrich Stelzl……………………………………………………………………………..33

E7-Klaus Zangger………………………………………………………………………….39

E8-Ellen L. Zechner………………………………………………………………………..46

E9-Rudolf Zechner…………………………………………………………………………52

E10-Robert Zimmermann………………………………………………………………….62

E1: R. Kühnlein

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Curriculum Vitae – Univ.- Prof. Dr. rer. nat. Ronald P. Kühnlein Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, 2nd floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 5504, E-Mail: [email protected] http://www.mpibpc.mpg.de/kuehnlein

Year of Birth *1966

Nationality German

Orcid ID http://orcid.org/ 0000-0003-1448-4117

No of Publications 36 (23 first and/or corresponding)

h-Index 22 (Scopus, April, 2018)

Ph.D. Students since 2013: 4 (3 finished, 1 ongoing, see Table 1)

Keywords Drosophila melanogaster, lipid metabolism, energy homeostasis,

lipases, lipid droplet proteins, inter-organ communication, hormonal

signaling, obesity

Professional Experiences

since 10/2016 University Professor for Biochemistry, Institute of Molecular

Biosciences, University of Graz, Austria

2010 - 2016 Independent Research Group head “Molecular Physiology”, Max Planck

Institute for biophysical Chemistry, Göttingen, Germany

2000 - 2009 Project group head, Department Molecular Developmental Biology, Max

Planck Institute for biophysical Chemistry, Göttingen, Germany

1998 - 1999 Research scientist, Max Planck Institute for biophysical Chemistry,

Göttingen, Germany

in 1998 Visiting scientist at the Rockefeller University, New York, USA

1997 - 1998 Postdoctoral fellow, Max Planck Institute for biophysical Chemistry,

Göttingen, Germany

1996 - 1997 Postdoctoral fellow, Georg-August-University, Göttingen, Germany

Education

2012 Habilitation in “Developmental Biology”, Georg-August-University,

Göttingen, Germany

1996 Dr. rer. nat., Eberhard-Karls-University, Tübingen, Germany

(“summa cum laude”)

1991 Diploma in Biology (“with distinction”; Subjects: Genetics, Microbiology,

Biochemistry), Ludwig-Maximilians-University, Munich, Germany

E1: R. Kühnlein

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Top 5 Oral Presentations

2017 Journal of Experimental Biology 2017 Symposium, Steyning, West Sussex,

UK

2016 FASEB conference, “Lipid droplets: Dynamic Organelles in Metabolism and

Beyond” Snowball, Colorado, USA

2016 28th Conference of European Comparative Endocrinologists, Leuven,

Belgium

2015 Invertebrate Neuropeptide Conference 2015, Bagan, Myanmar

2015 9th International Congress of Comparative Physiology and

Biochemistry, Kraków, Poland

Top 5 Career Related Activities in Editorial Boards, as Reviewers and Memberships

since 2000 Reviewer of 35 peer-reviewed Journals (“top ten” according to IF: Nature,

Nature Methods, Cell Metabolism, Trends in Cell Biology, Nature

Communications, EMBO Journal, PNAS, Trends in Endocrinology and

Metabolism, PLoS Genetics, Cell Reports)

2012 Guest editor PLoS Genetics

since 2000 Scientific reviewer for research foundations [FWF (AT), DFG (DE), Welcome

Trust (GB), NWO (NL), GACR (CZ), HFSP (FR), Boehringer Ingelheim Fund

(DE), DAAD (DE)] and Universities [University of Rochester (USA),

Washington University School of Medicine (USA), University of Graz (AT)]

2003/2009 Conference organization: European Drosophila Research Conferences

(Göttingen, Nice)

since 2016 Society membership: Deutsche Gesellschaft für Fettwissenschaft (DGF)

Externally Funded, Competitive Research Grants (since 2013)

Funding Org.

Project Number

Research Topic Amount Funded [kEuro]

Funding Period

DFG KU 2866/1-1 Regulation of Drosophila

melanogaster body fat content by store-operated calcium entry

133 2013-2016

Behrens Weise

Foundation

Nutrigenomics of Obesity using the Drosophila Model Organism

150 2013-2015

International Collaborations

Rolando RIVERA-POMAR, Universidad Nacional del Noroeste de Buenos Aires / National

Science and Technology Research Council (CONICET), Pergamino, Argentina

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Michael WELTE, University of Rochester, Rochester, USA

Xun HUANG, Chinese Academy of Sciences, Beijing, People`s Republic of China

Christian WEGENER, Universität Würzburg, Würzburg, Germany

Radmila ČAPKOVÁ FRYDRYCHOVÁ, Czech Academy of Sciences, Czech Republic

Bruno LEMAITRE, École Polytechnique Fédérale de Lausanne, Switzerland

International Collaborations relevant for doc.fund MOBILES

J. VANDEN BROEK, KV Leuven, Belgium: Akh-TGL in migratory locusts

D. KODRIK, Biology Centre Czech Academy of Sciences, Czech Republic: Systemic Akh-

TGL modulators

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List of Publications since 1. 1. 2013 (peer reviewed)

[1] Musselman LP, Kuhnlein RP. Drosophila as a model to study obesity and metabolic disease. J Exp Biol 2018; 221. DOI: 10.1242/jeb.163881

[2] Rockenfeller P, Smolnig M, Diessl J, Bashir M, Schmiedhofer V, Knittelfelder O, Ring J, Franz J, Foessl I, Khan MJ, Rost R, Graier WF, Kroemer G, Zimmermann A, Carmona-Gutierrez D, Eisenberg T, Buttner S, Sigrist SJ, Kuhnlein RP, Kohlwein SD, Gourlay CW, Madeo F. Diacylglycerol triggers Rim101 pathway-dependent necrosis in yeast: a model for lipotoxicity. Cell Death Differ 2018; 25:765-81. DOI: 10.1038/s41418-017-0014-2

[3] Korandova M, Krucek T, Szakosova K, Kodrik D, Kuhnlein RP, Tomaskova J, Capkova Frydrychova R. Chronic low-dose pro-oxidant treatment stimulates transcriptional activity of telomeric retroelements and increases telomere length in Drosophila. J Insect Physiol 2018; 104:1-8. DOI: 10.1016/j.jinsphys.2017.11.002

[4] Galikova M, Klepsatel P, Munch J, Kuhnlein RP. Spastic paraplegia-linked phospholipase PAPLA1 is necessary for development, reproduction, and energy metabolism in Drosophila. Sci Rep 2017; 7:46516 DOI: 10.1038/srep46516

[5] Gáliková, M, Klepsatel, P, Xu, Y, and Kühnlein, RP. The obesity-related Adipokinetic hormone controls feeding and expression of neuropeptide regulators of Drosophila metabolism Eur J Lipid Sci Technol 2016, 118. DOI: 10.1002/ejlt.201600138

[6] Klepsatel P, Gáliková M, Xu Y, and Kühnlein RP. Thermal stress depletes energy reserves in Drosophila Sci Rep 2016, 6. DOI: 10.1038/srep33667

[7] Gáliková M, Diesner M, Klepsatel P, Hehlert P, Xu Y, Bickmeyer I, Predel R, and Kühnlein RP. Energy Homeostasis Control in Drosophila Adipokinetic Hormone Mutants Genetics 2015, 201 (2), 665-683. DOI: 10.1534/genetics.115.178897

[8] Baumbach J, Xu Y, Hehlert P, and Kühnlein RP. Gαq, Gγ1 and Plc21C control Drosophila body fat storage J of Genet and Genomics 2014, 41 (5), 283-292. DOI: 10.1016/j.jgg.2014.03.005

[9] Baumbach J, Hummel P, Bickmeyer I, Kowalczyk KM, Frank M, Knorr K, Hildebrandt A, Riedel D, Jäckle H, and Kühnlein RP. A Drosophila in vivo screen identifies store-operated calcium entry as a key regulator of adiposity Cell Metab 2014, 19, 331-343. DOI: 10.1016/j.cmet.2013.12.004

10 Most Relevant Publications (Entire Career)

[1] Gáliková, M., Diesner, M., Klepsatel, P., Hehlert, P., Xu, Y., Bickmeyer, I., Predel, R., and Kühnlein, R. P. Energy Homeostasis Control in Drosophila Adipokinetic Hormone Mutants Genetics 2015, 201 (2), 665-683. DOI: 10.1534/genetics.115.178897

[2] Baumbach, J., Hummel, P., Bickmeyer, I., Kowalczyk, K.M., Frank, M., Knorr, K., Hildebrandt, A., Riedel, D., Jäckle, H., and Kühnlein, R. P. A Drosophila in vivo screen identifies store-operated calcium entry as a key regulator of adiposity Cell Metab 2014, 19, 331-343. DOI: 10.1016/j.cmet.2013.12.004

[3] Birner-Gruenberger, R., Lange, J., Bickmeyer, I., Hermetter, A., Kollroser, M., Rechberger G.N., and Kühnlein, R. P. Functional fat body proteomics and gene targeting reveal in vivo functions of Drosophila melanogaster α-Esterase-7 Insect Biochem Mol Biol 2012, 42, 220-229. DOI: 10.1016/j.ibmb.2011.12.004

[4] Kühnlein, R. P. Lipid droplet-based Storage Fat Metabolism in Drosophila J Lipid Res 2012, 53, 1430-1436. DOI: 10.1016/j.plipres.2011.04.001

[5] Kühnlein, R. P. 2011: The contribution of the Drosophila model to lipid droplet research Prog in Lipid Res 2011, 50 (4), 348-356. DOI:

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10.1016/j.plipres.2011.04.001

[6] Beller, M., Bulankina, A.V., Hsiao, H.-H., Urlaub, H., Jäckle, H., and Kühnlein, R. P. PERILIPIN-dependent Control of Lipid Droplet Structure and Fat Storage in Drosophila Cell Metab 2010, 12 (5), 521-532. DOI: 10.1016/j.cmet.2010.10.001

[7] Grönke, S., Müller, G., Hirsch, J., Fellert, S., Andreou, A., Haase, T., Jäckle, H., and Kühnlein, R. P. Dual lipolytic control of body fat storage and mobilization in Drosophila PLoS Biol 2007, 5 (6), e137. DOI: 10.1371/journal.pbio.0050137

[8] Beller, M., Riedel, D., Jänsch, L., Dieterich, G., Wehland, J., Jäckle, H. and Kühnlein, R. P. Characterization of the Drosophila lipid droplet subproteome Mol Cell Proteomics 2006, 5 (6), 1082-1094. DOI: 10.1074/mcp.M600011-MCP200

[9] Grönke, S., Mildner, A., Fellert, S., Tennagels, N., Petry, S., Müller, G., Jäckle, H. and Kühnlein, R. P. Brummer lipase is an evolutionary conserved fat storage regulator in Drosophila Cell Metab 2005, 1 (5), 323-330. DOI: 10.1016/j.cmet.2005.04.003

[10] Grönke, S., Beller, M., Fellert, S., Ramakrishnan, H., Jäckle, H. and Kühnlein, R. P. Control of Fat Storage by a Drosophila PAT Domain Protein Current Biol 2003, 13 (7), 603-606. DOI: 10.1016/S0960-9822(03)00175-1

E2: F. Madeo

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Curriculum Vitae – Univ.-Prof. Dr. rer. nat. Frank Madeo Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, ground floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 8878, E-Mail: [email protected] https://molekularbiologie.uni-graz.at/de/alterung-zelltod-labor-frank-madeo/

Year of Birth *1967

Nationality German

Orcid ID orcid.org/0000-0002-5070-1329


h-Index 65 (Scopus, April 2018)


Keywords apoptosis, autophagy, epigenetics and aging in yeast, flies and

mammalian model systems


since 2004 Full Professor at the University of Graz, Austria

1997 - 2004 Group Leader at the University of Tübingen, Germany

Education

1994 - 1997 Ph.D. thesis “Tyrosin phosphorylation and apoptosis in yeast”,

University of Tuebingen, Germany, with first class honors

1987 - 1993 Studies of Biochemistry, University of Tübingen, Germany


2017 Mosbacher Kolloquium, Mosbach, Germany

2017 Helmholtz- Nature Medicine Diabetes conference, Munich, Germany

2016 Invitation of behalf of the Swedish Royal Academy of Science,

Goetheborg, Sweden

2014 Keystone Symposium on Autophagy, Austin, TX, USA

2012 EMBO conference on “Programmed Cell Death in Model Organisms” Ein

Gedi, Israel

Honors ▪ Awards

2015 Erzherzog-Johann Research Award

2003 - 2004 Heisenberg fellow of the Deutsche Forschungsgemeinschaft

mailto:[email protected]

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since 2018 Member of the „American Academy of Microbiology”

since 2017 Executive Editor at “Cell Stress”

since 2014 Editor in Chief “Microbial Cell”

2009 Best publishing scientist of the University of Graz

since 2007 Vice Speaker of the FWF-funded research consortium SFB LIPOTOX

Top 5 Funded Projects


Funding Org.

Project Number

Research Topic Amount funded [kEuro]

Funding Period

FWF P 29203 Causal epigenetic changes during

aging and caloric restriction 352 2016-2019

FWF P 29262 Mechanisms of Spermidine mediated neuroprotection

339 2016-2019

FWF P 27893 Pro-autophagic Polyphenols and

Polyamines for Longevity 453 2015-2018

Austrian Ministry of Science/ University support

Unconventional research

programm 420 2014-2017

FWF W1226 DK Metabolic and Cardiovascular

Disease 392 2010-2018

International Collaborations

Guido KROEMER, Centre de Recherche des Cordoliers, France

Jörn DENGJEL, Université de Fribourg, Department Biology, Fribourg, Switzerland

Stephan SIGRIST, Institute for Biology/ Genetics, Freie Universität Berlin, Germany

Chris MEISINGER, Institut für Biochemie und Molekularbiologie, University of Freiburg,

Germany

Rafael de CABO, National Institutes of Health (NIH), Baltimore, USA


Guido KROEMER, Centre de Recherche des Cordoliers, France: Investigation of epigenetics

in cell culture

Chris MEISINGER, Institut für Biochemie und Molekularbiologie, University of Freiburg,

Germany: Mitochondrial aspects of spermidine functions

https://www.researchgate.net/institution/Universite_de_Fribourg

https://www.researchgate.net/institution/Universite_de_Fribourg/department/Departement_de_Biologie

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[1] Sauvat A, Chen G, Müller K, Tong M, Aprahamian F, Durand S, Cerrato G, Bezu L, Leduc M, Franz J, Rockenfeller P, Sadoshima J, Madeo F, Kepp O, and Kroemer G. Trans-Fats Inhibit Autophagy Induced by Saturated Fatty Acids. EBioMedicine 2018 DOI: 10.1016/j.ebiom.2018.03.028

[2] Rockenfeller P, Smolnig M, Diessl J, Bashir M, Schmiedhofer V, Knittelfelder O, Ring J, Franz J, Foessl I, Khan MJ, Rost R, Graier WF, Kroemer G, Zimmermann A, Carmona-Gutierrez D, Eisenberg T, Büttner S, Sigrist SJ, Kühnlein RP, Kohlwein SD, Gourlay CW, and Madeo F. Diacylglycerol triggers Rim101 pathway-dependent necrosis in yeast: a model for lipotoxicity. Cell Death Differ 2018 25(4): 765–781. DOI: 10.1038/s41418-017-0014-2

[3] Pietrocola F, Castoldi F, Markaki M, Lachkar S, Chen G, Enot DP, Durand S, Bossut N, Tong M, Malik SA, Loos F, Dupont N, Mariño G, Abdelkader N, Madeo F, Maiuri MC, Kroemer R, Codogno P, Sadoshima J, Tavernarakis N, and Kroemer G. Aspirin Recapitulates Features of Caloric Restriction. Cell Rep 2018 22 (9): 2395–2407. DOI: 10.1016/j.celrep.2018.02.024

[4] Madeo F, Eisenberg T, Pietrocola F, and Kroemer G. Spermidine in health and disease. Science 2018 359(6374). doi: 10.1126/science.aan2788

[5] Guaragnella N, Stirpe M, Burhans W, Côrte-Real M, Gourlay C, Ludovico P, Madeo F, Petranovic D, Winderickx J, Mazzoni C, and Giannattasio S. New perspectives from South-Y-East, not all about death. A report of the 12thlnternational Meeting on Yeast Apoptosis in Bari, Italy, May 14th-18th, 2017. Microb Cell 2018 5 (2): 112–115. DOI: 10.15698/mic2018.02.616

[6] Schwarz C, Stekovic S, Wirth M, Benson G, Royer P, Sigrist SJ, Pieber T, Dammbrueck C, Magnes C, Eisenberg T, Pendl T, Bohlken J, Köbe T, Madeo F, and Flöel A. Safety and tolerability of spermidine supplementation in mice and older adults with subjective cognitive decline. Aging (Albany NY) 2018) 10 (1): 19–33. DOI: 10.18632/aging.101354

[7] Ring J, Rockenfeller P, Abraham C, Tadic J, Poglitsch M, Schimmel K, Westermayer J, Schauer S, Achleitner B, Schimpel C, Moitzi B, Rechberger GN, Sigrist SJ, Carmona-Gutierrez D, Kroemer G, Büttner S, Eisenberg T, and Madeo F. Mitochondrial energy metabolism is required for lifespan extension by the spastic paraplegia-associated protein spartin. Microb Cell 2017 4(12): 411–422. DOI: 10.15698/mic2017.12.603

[8] Zechner R, Madeo F, and Kratky D. Cytosolic lipolysis and lipophagy: two sides of the same coin. Nat Rev Mol Cell Biol 2017. 18(11): 671–684. doi: 10.1038/nrm.2017.76

[9] Stekovic S, Ruckenstuhl C, Royer P, Winkler-Hermaden C, Carmona-Gutierrez D, Fröhlich K-U, Kroemer G, and Madeo F. The neuroprotective steroid progesterone promotes mitochondrial uncoupling, reduces cytosolic calcium and augments stress resistance in yeast cells. Microb Cell 2017 4 (6): 191–199. DOI: 10.15698/mic2017.06.577

[10] Kainz K, Tadic J, Zimmermann A, Pendl T, Carmona-Gutierrez D, Ruckenstuhl C, Eisenberg T, and Madeo F. Methods to Assess Autophagy and Chronological Aging in Yeast. Meth Enzymol 2017 588: 367–394. DOI: 10.1016/bs.mie.2016.09.086

[11] Pietrocola F, Demont Y, Castoldi F, Enot D, Durand S, Semeraro M, Baracco EE, Pol J, Bravo-San Pedro JM, Bordenave C, Levesque S, Humeau J, Chery A, Métivier D, Madeo

https://doi.org/10.1016/j.ebiom.2018.03.028

https://doi.org/10.1038/s41418-017-0014-2

https://doi.org/10.1038/s41418-017-0014-2

https://doi.org/10.1016/j.celrep.2018.02.024

https://doi.org/10.1126/science.aan2788

https://doi.org/10.15698/mic2018.02.616

https://doi.org/10.18632/aging.101354

https://doi.org/10.15698/mic2017.12.603

https://doi.org/10.1038/nrm.2017.76

https://doi.org/10.15698/mic2017.06.577

https://doi.org/10.1016/bs.mie.2016.09.086

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F, Maiuri MC and Kroemer G. Metabolic effects of fasting on human and mouse blood in vivo Autophagy 2017, 6,1-12. DOI: 10.1080/15548627.2016.1271513

[12] Eisenberg T, Abdellatif M, Schroeder S, Primessnig U, Stekovic S, Pendl T, Harger A, Schipke J, Zimmermann A, Schmidt A, Tong M, Ruckenstuhl C, Dammbrueck C, Gross AS, Herbst V, Magnes C, Trausinger G, Narath S, Meinitzer A, Hu Z, Kirsch A, Eller K, Carmona-Gutierrez D, Büttner S, Pietrocola F, Knittelfelder O, Schrepfer E, Rockenfeller P, Simonini C, Rahn A, Horsch M, Moreth K, Beckers J, Fuchs H, Gailus-Durner V, Neff F, Janik D, Rathkolb B, Rozman J, de Angelis MH, Moustafa T, Haemmerle G, Mayr M, Willeit P, von Frieling-Salewsky M, Pieske B, Scorrano L, Pieber T, Pechlaner R, Willeit J, Sigrist SJ, Linke WA, Mühlfeld C, Sadoshima J, Dengjel J, Kiechl S, Kroemer G, Sedej S and Madeo F. Cardioprotection and lifespan extension by the natural polyamine spermidine Nat Med 2016, 22(12), 1428-1438. DOI: 10.1038/nm.4222

[13] Carmona-Gutierrez D, Hughes AL, Madeo F, and Ruckenstuhl C. The crucial impact of lysosomes in aging and longevity. Ageing Res Rev 2016. 32: 2–12. doi:10.1016/j.arr.2016.04.009

[14] Macheiner T, Fengler VH, Agreiter M, Eisenberg T, Madeo F, Kolb D, Huppertz B, Ackbar R and Sargsyan K. Magnetomitotransfer: An efficient way for direct mitochondria transfer into cultured human cells. Sci Rep 2016, 21, 6-35571. DOI: 10.1038/srep35571

[15] Gupta VK, Pech U, Bhukel A, Fulterer A, Ender A, Mauermann SF, Andlauer TF, Antwi-Adjei E, Beuschel C, Thriene K, Maglione M, Quentin C, Bushow R, Schwärzel M, Mielke T,Madeo F, Dengjel J, Fiala A and Sigrist SJ. Spermidine Suppresses Age-Associated Memory Impairment by Preventing Adverse Increase of Presynaptic Active Zone Size and Release PLoS Biol 2016, 29, 14(9), e1002563. DOI: 10.1371/journal.pbio.1002563

[16] Pietrocola F, Pol J, Vacchelli E, Baracco EE, Levesque S, Castoldi F, Maiuri MC, Madeo F and Kroemer G. Autophagy induction for the treatment of cancer Autophagy 2016, 12(10), 1962-1964

[17] López-Otín C, Galluzzi L, Freije JM, Madeo F and Kroemer G. Metabolic Control of Longevity Cell 2016, 11, 166(4), 802-21. DOI: 10.1016/j.cell.2016.07.031

[18] Pietrocola F, Pol J, Vacchelli E, Rao S, Enot DP, Baracco EE, Levesque S, Castoldi F, Jacquelot N, Yamazaki T, Senovilla L, Marino G, Aranda F, Durand S, Sica V, Chery A, Lachkar S, Sigl V, Bloy N, Buque A, Falzoni S, Ryffel B, Apetoh L, Di Virgilio F, Madeo F, Maiuri MC, Zitvogel L, Levine B, Penninger JM and Kroemer G. Caloric Restriction Mimetics Enhance Anticancer Immunosurveillance Cancer Cell 2016, 11, 30(1), 147-60. DOI: 10.1016/j.ccell.2016.05.016

[19] Mitchell SJ, Madrigal-Matute J, Scheibye-Knudsen M, Fang E, Aon M, González-Reyes JA, Cortassa S, Kaushik S, Gonzalez-Freire M, Patel B, Wahl D, Ali A, Calvo-Rubio M, Burón MI, Guiterrez V, Ward TM, Palacios HH, Cai H, Frederick DW, Hine C, Broeskamp F, Habering L, Dawson J, Beasley TM, Wan J, Ikeno Y, Hubbard G, Becker KG, Zhang Y, Bohr VA, Longo DL, Navas P, Ferrucci L, Sinclair DA, Cohen P, Egan JM, Mitchell JR, Baur JA, Allison DB, Anson RM, Villalba JM, Madeo F, Cuervo AM, Pearson KJ, Ingram DK, Bernier M and de Cabo R. Effects of Sex, Strain, and Energy Intake on Hallmarks of Aging in Mice Cell Metab 2016, 14, 23(6), 1093-112. DOI: 10.1016/j.cmet.2016.05.027

[20] Rockenfeller P, Carmona-Gutierrez D, Pietrocola F, Kroemer G, and Madeo F. Ethanolamine: A novel anti-aging agent Mol Cell Oncol 2016, 3 (1), e1019023. DOI: 10.1080/23723556.2015.1019023

https://doi.org/10.1016/j.arr.2016.04.009

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[21] Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Acevedo Arozena A, Adachi H, Adams CM, Adams PD, Adeli K, Adhihetty PJ, Adler SG, Agam G, Agarwal R, Aghi MK, Agnello M, Agostinis P, Aguilar PV, Aguirre-Ghiso J, Airoldi EM, Ait-Si-Ali S, Akematsu T, Akporiaye ET, Al-Rubeai M, Albaiceta GM, Albanese C, Albani D, Albert ML, Aldudo J, Algül H, Alirezaei M, Alloza I, Almasan A, Almonte-Beceril M, Alnemri ES, Alonso C, Altan-Bonnet N, Altieri DC, Alvarez S, Alvarez-Erviti L, Alves S, Amadoro G, Amano A, Amantini C, Ambrosio S, Amelio I, Amer AO, Amessou M, Amon A, An Z, Anania FA, Andersen SU, Andley UP, Andreadi CK, Andrieu-Abadie N, Anel A, Ann DK, Anoopkumar-Dukie S, Antonioli M, Aoki H, Apostolova N, Aquila S, Aquilano K, Araki K, Arama E, Aranda A, Araya J, Arcaro A, Arias E, Arimoto H, Ariosa AR, Armstrong JL, Arnould T, Arsov I, Asanuma K, ….Madeo F, … Zhou S-F, Zhou X-J, Zhu H, Zhu H, Zhu W-G, Zhu W, Zhu X-F, Zhu Y, Zhuang S-M, Zhuang X, Ziparo E, Zois CE, Zoladek T, Zong W-X, Zorzano A, and Zughaier SM. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) Autophagy 2016, 12(1), 1–222. DOI: 10.1080/15548627.2015.1100356

[22] Enot DP, Niso-Santano M, Durand S, Chery A, Pietrocola F, Vacchelli E, Madeo F, Galluzzi L, and Kroemer G. Metabolomic analyses reveal that anti-aging metabolites are depleted by palmitate but increased by oleate in vivo Cell Cycle 2015, 14(15), 2399–2407. DOI: 10.1080/15384101.2015.1064206

[23] Longo VD, Antebi A, Bartke A, Barzilai N, Brown-Borg HM, Caruso C, Curiel TJ, de Cabo R, Franceschi C, Gems D, Ingram DK, Johnson TE, Kennedy BK, Kenyon C, Klein S, Kopchick JJ, Lepperdinger G, Madeo F, Mirisola MG, Mitchell JR, Passarino G, Rudolph KL, Sedivy JM, Shadel GS, Sinclair DA, Spindler SR, Suh Y, Vijg J, Vinciguerra M, and Fontana L. Interventions to Slow Aging in Humans: Are We Ready? Aging Cell 2015, 14(4), 497–510. DOI: 10.1111/acel.12338

[24] Pietrocola F, Galluzzi L, Bravo-San Pedro JM, Madeo F, and Kroemer G. Acetyl coenzyme A: a central metabolite and second messenger Cell Metab 2015, 21 (6), 805–821. DOI: 10.1016/j.cmet.2015.05.014

[25] Carmona-Gutierrez D, Zimmermann A, and Madeo F. A molecular mechanism for lipophagy regulation in the liver Hepatology 2015, 61 (6), 1781–1783. DOI: 10.1002/hep.27738

[26] Libiseller G, Dvorzak M, Kleb U, Gander E, Eisenberg T, Madeo F, Neumann S, Trausinger G, Sinner F, Pieber T, and Magnes. IPO: a tool for automated optimization of XCMS parameters BMC Bioinformatics 12015, 6, 118. DOI: 10.1186/s12859-015-0562-8

[27] Niso-Santano M, Malik SA, Pietrocola F, Bravo-San Pedro JM, Mariño G, Cianfanelli V, Ben-Younès A, Troncoso R, Markaki M, Sica V, Izzo V, Chaba K, Bauvy C, Dupont N, Kepp O, Rockenfeller P, Wolinski H, Madeo F, Lavandero S, Codogno P, Harper F, Pierron G, Tavernarakis N, Cecconi F, Maiuri MC, Galluzzi L, and Kroemer G. Unsaturated fatty acids induce non-canonical autophagy EMBO J 2015, 34 (8), 1025–1041. DOI: 10.15252/embj.201489363

[28] Braun RJ, Sommer C, Leibiger C, Gentier RJG, Dumit VI, Paduch K, Eisenberg T, Habernig L, Trausinger G, Magnes C, Pieber T, Sinner F, Dengjel J, van Leeuwen FW, Kroemer G, and Madeo F. Accumulation of Basic Amino Acids at Mitochondria Dictates the Cytotoxicity of Aberrant Ubiquitin Cell Rep 2015, DOI: 10.1016/j.celrep.2015.02.009

[29] Rockenfeller P, Koska M, Pietrocola F, Minois N, Knittelfelder O, Sica V, Franz J, Carmona-Gutierrez D, Kroemer G, and Madeo F. Phosphatidylethanolamine positively

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regulates autophagy and longevity Cell Death Differ 2015, 22 (3), 499–508. DOI: 10.1038/cdd.2014.219

[30] Pietrocola F, Lachkar S, Enot DP, Niso-Santano M, Bravo-San Pedro JM, Sica V, Izzo V, Maiuri MC, Madeo F, Mariño G, and Kroemer G. Spermidine induces autophagy by inhibiting the acetyltransferase EP300 Cell Death Differ 2015, 22 (3), 509–516. DOI: 10.1038/cdd.2014.215

[31] Hine C, Harputlugil E, Zhang Y, Ruckenstuhl C, Lee BC, Brace L, Longchamp A, Treviño-Villarreal JH, Mejia P, Ozaki CK, Wang R, Gladyshev VN, Madeo F, Mair WB, and Mitchell JR. Endogenous hydrogen sulfide production is essential for dietary restriction benefits Cell 2015, 160(1–2), 132–144. DOI: 10.1016/j.cell.2014.11.048

[32] Madeo F, Zimmermann A, Maiuri MC, and Kroemer G. Essential role for autophagy in life span extension J Clin Invest 2015, 125(1), 85–93. DOI: 10.1172/JCI73946

[33] Galluzzi L, Bravo-San Pedro JM, Vitale I, Aaronson SA, Abrams JM, Adam D, Alnemri ES, Altucci L, Andrews D, Annicchiarico-Petruzzelli M, Baehrecke EH, Bazan NG, Bertrand MJ, Bianchi K, Blagosklonny MV, Blomgren K, Borner C, Bredesen DE, Brenner C, Campanella M, Candi E, Cecconi F, Chan FK, Chandel NS, Cheng EH, Chipuk JE, Cidlowski JA, Ciechanover A, Dawson TM, Dawson VL, De Laurenzi V, De Maria R, Debatin K-M, Di Daniele N, Dixit VM, Dynlacht BD, El-Deiry WS, Fimia GM, Flavell RA, Fulda S, Garrido C, Gougeon M-L, Green DR, Gronemeyer H, Hajnoczky G, Hardwick JM, Hengartner MO, Ichijo H, Joseph B, Jost PJ, Kaufmann T, Kepp O, Klionsky DJ, Knight RA, Kumar S, Lemasters JJ, Levine B, Linkermann A, Lipton SA, Lockshin RA, López-Otín C, Lugli E, Madeo F, Malorni W, Marine J-C, Martin SJ, Martinou J-C, Medema JP, Meier P, Melino S, Mizushima N, Moll U, Muñoz-Pinedo C, Nuñez G, Oberst A, Panaretakis T, Penninger JM, Peter ME, Piacentini M, Pinton P, Prehn JH, Puthalakath H, Rabinovich GA, Ravichandran KS, Rizzuto R, Rodrigues CM, Rubinsztein DC, Rudel T, Shi Y, Simon H-U, Stockwell BR, Szabadkai G, Tait SW, Tang HL, Tavernarakis N, Tsujimoto Y, Vanden Berghe T, Vandenabeele P, Villunger A, Wagner EF, Walczak H, White E, Wood WG, Yuan J, Zakeri Z, Zhivotovsky B, Melino G, and Kroemer G. Essential versus accessory aspects of cell death: recommendations of the NCCD Cell Death Differ 2015, 22(1), 58–73. DOI: 10.1038/cdd.2014.137

[34] Niso-Santano M, Bravo-San Pedro JM, Maiuri MC, Tavernarakis N, Cecconi F, Madeo F, Codogno P, Galluzzi L, and Kroemer G. Novel inducers of BECN1-independent autophagy: cis-unsaturated fatty acids Autophagy 2015, 11(3), 575–577. DOI: 10.1080/15548627.2015.1017222

[35] Garg AD, Galluzzi L, Apetoh L, Baert T, Birge RB, Bravo-San Pedro JM, Breckpot K, Brough D, Chaurio R, Cirone M, Coosemans A, Coulie PG, De Ruysscher D, Dini L, de Witte P, Dudek-Peric AM, Faggioni A, Fucikova J, Gaipl US, Golab J, Gougeon M-L, Hamblin MR, Hemminki A, Herrmann M, Hodge JW, Kepp O, Kroemer G, Krysko DV, Land WG, Madeo F, Manfredi AA, Mattarollo SR, Maueroder C, Merendino N, Multhoff G, Pabst T, Ricci J-E, Riganti C, Romano E, Rufo N, Smyth MJ, Sonnemann J, Spisek R, Stagg J, Vacchelli E, Vandenabeele P, Vandenberk L, Van den Eynde BJ, Van Gool S, Velotti F, Zitvogel L, and Agostinis P. Molecular and Translational Classifications of DAMPs in Immunogenic Cell Death Front Immunol 2015, 6, 588. DOI: 10.3389/fimmu.2015.00588

[36] Kozieł R, Ruckenstuhl C, Albertini E, Neuhaus M, Netzberger C, Bust M, Madeo F, Wiesner RJ, and Jansen-Dürr P. Methionine restriction slows down senescence in human diploid fibroblasts Aging Cell 2014, 13 (6), 1038–1048. DOI: 10.1111/acel.12266

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[37] Mossmann D, Vögtle F-N, Taskin AA, Teixeira PF, Ring J, Burkhart JM, Burger N, Pinho CM, Tadic J, Loreth D, Graff C, Metzger F, Sickmann A, Kretz O, Wiedemann N, Zahedi RP, Madeo F, Glaser E, and Meisinger C. Amyloid-β peptide induces mitochondrial dysfunction by inhibition of preprotein maturation Cell Metab 2014, 20 (4), 662–669. DOI: 10.1016/j.cmet.2014.07.024

[38] Madeo F, Pietrocola F, Eisenberg T, and Kroemer G. Caloric restriction mimetics: towards a molecular definition Nat Rev Drug Discov 2014, 13 (10), 727–740. DOI: 10.1038/nrd4391

[39] Kepp O, Senovilla L, Vitale I, Vacchelli E, Adjemian S, Agostinis P, Apetoh L, Aranda F, Barnaba V, Bloy N, Bracci L, Breckpot K, Brough D, Buqué A, Castro MG, Cirone M, Colombo MI, Cremer I, Demaria S, Dini L, Eliopoulos AG, Faggioni A, Formenti SC, Fučíková J, Gabriele L, Gaipl US, Galon J, Garg A, Ghiringhelli F, Giese NA, Guo ZS, Hemminki A, Herrmann M, Hodge JW, Holdenrieder S, Honeychurch J, Hu H-M, Huang X, Illidge TM, Kono K, Korbelik M, Krysko DV, Loi S, Lowenstein PR, Lugli E, Ma Y, Madeo F, Manfredi AA, Martins I, Mavilio D, Menger L, Merendino N, Michaud M, Mignot G, Mossman KL, Multhoff G, Oehler R, Palombo F, Panaretakis T, Pol J, Proietti E, Ricci J-E, Riganti C, Rovere-Querini P, Rubartelli A, Sistigu A, Smyth MJ, Sonnemann J, Spisek R, Stagg J, Sukkurwala AQ, Tartour E, Thorburn A, Thorne SH, Vandenabeele P, Velotti F, Workenhe ST, Yang H, Zong W-X, Zitvogel L, Kroemer G, and Galluzzi L. Consensus guidelines for the detection of immunogenic cell death Oncoimmunology 2014, 3 (9), e955691. DOI: 10.4161/21624011.2014.955691

[40] de Cabo R, Carmona-Gutierrez D, Bernier M, Hall MN, and Madeo F. The search for antiaging interventions: from elixirs to fasting regimens Cell 2014, 157 (7), 1515–1526. DOI: 10.1016/j.cell.2014.05.031

[41] Eisenberg T, Schroeder S, Büttner S, Carmona-Gutierrez D, Pendl T, Andryushkova A, Mariño G, Pietrocola F, Harger A, Zimmermann A, Magnes C, Sinner F, Sedej S, Pieber TR, Dengjel J, Sigrist S, Kroemer G, and Madeo F. A histone point mutation that switches on autophagy Autophagy 2014, 10(6), 1143–1145. DOI: 10.4161/auto.28767

[42] Ruckenstuhl C, Netzberger C, Entfellner I, Carmona-Gutierrez D, Kickenweiz T, Stekovic S, Gleixner C, Schmid C, Klug L, Sorgo AG, Eisenberg T, Büttner S, Mariño G, Koziel R, Jansen-Dürr P, Fröhlich K-U, Kroemer G, and Madeo F. Lifespan extension by methionine restriction requires autophagy-dependent vacuolar acidification PLoS Genet 2014, 10 (5), e1004347. DOI: 10.1371/journal.pgen.1004347

[43] Mariño G, Pietrocola F, Eisenberg T, Kong Y, Malik SA, Andryushkova A, Schroeder S, Pendl T, Harger A, Niso-Santano M, Zamzami N, Scoazec M, Durand S, Enot DP, Fernández ÁF, Martins I, Kepp O, Senovilla L, Bauvy C, Morselli E, Vacchelli E, Bennetzen M, Magnes C, Sinner F, Pieber T, López-Otín C, Maiuri MC, Codogno P, Andersen JS, Hill JA, Madeo F, and Kroemer G. Regulation of autophagy by cytosolic acetyl-coenzyme A Mol Cell 2014, 53(5), 710–725. DOI: 10.1016/j.molcel.2014.01.016

[44] Eisenberg T, Schroeder S, Andryushkova A, Pendl T, Küttner V, Bhukel A, Mariño G, Pietrocola F, Harger A, Zimmermann A, Moustafa T, Sprenger A, Jany E, Büttner S, Carmona-Gutierrez D, Ruckenstuhl C, Ring J, Reichelt W, Schimmel K, Leeb T, Moser C, Schatz S, Kamolz L-P, Magnes C, Sinner F, Sedej S, Fröhlich K-U, Juhasz G, Pieber TR, Dengjel J, Sigrist SJ, Kroemer G, and Madeo F. Nucleocytosolic depletion of the energy metabolite acetyl-coenzyme a stimulates autophagy and prolongs lifespan Cell Metab 2014, 19 (3), 431–444. DOI: 10.1016/j.cmet.2014.02.010

[45] Magnes C, Fauland A, Gander E, Narath S, Ratzer M, Eisenberg T, Madeo F, Pieber T, and Sinner F. Polyamines in biological samples: rapid and robust quantification by solid-

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phase extraction online-coupled to liquid chromatography-tandem mass spectrometry.J Chromatogr A 2014, 1331, 44–51. DOI: 10.1016/j.chroma.2013.12.061

[46] Sukkurwala AQ, Martins I, Wang Y, Schlemmer F, Ruckenstuhl C, Durchschlag M, Michaud M, Senovilla L, Sistigu A, Ma Y, Vacchelli E, Sulpice E, Gidrol X, Zitvogel L, Madeo F, Galluzzi L, Kepp O, and Kroemer G. Immunogenic calreticulin exposure occurs through a phylogenetically conserved stress pathway involving the chemokine CXCL8 Cell Death Differ 2014, 21(1), 59–68. DOI: 10.1038/cdd.2013.73

[47] Sigrist SJ, Carmona-Gutierrez D, Gupta VK, Bhukel A, Mertel S, Eisenberg T, and Madeo F. Spermidine-triggered autophagy ameliorates memory during aging Autophagy 2014, 10(1), 178–179. DOI: 10.4161/auto.26918

[48] Pietrocola F, Malik SA, Mariño G, Vacchelli E, Senovilla L, Chaba K, Niso-Santano M, Maiuri MC, Madeo F, and Kroemer G. Coffee induces autophagy in vivo Cell Cycle 2014, 13(12), 1987–1994. DOI: 10.4161/cc.28929

[49] Mariño G, Pietrocola F, Madeo F, and Kroemer G. Caloric restriction mimetics: natural/physiological pharmacological autophagy inducers Autophagy 2014, 10(11), 1879–1882. DOI: 10.4161/auto.36413

[50] Büttner S, Broeskamp F, Sommer C, Markaki M, Habernig L, Alavian-Ghavanini A, Carmona-Gutierrez D, Eisenberg T, Michael E, Kroemer G, Tavernarakis N, Sigrist SJ, and Madeo F. Spermidine protects against α-synuclein neurotoxicity Cell Cycle 2014, 13(24), 3903–3908. DOI: 10.4161/15384101.2014.973309

[51] Stekovic S and Madeo F. Cell death pathways Biochim Biophys Acta 2013, 1833(12): 3447. DOI: 10.1016/j.bbamcr.2013.09.016

[52] Büttner S, Habernig L, Broeskamp F, Ruli D, Vögtle FN, Vlachos M, Macchi F, Küttner V, Carmona-Gutierrez D, Eisenberg T, Ring J, Markaki M, Taskin AA, Benke S, Ruckenstuhl C, Braun R, Van den Haute C, Bammens T, van der Perren A, Fröhlich K-U, Winderickx J, Kroemer G, Baekelandt V, Tavernarakis N, Kovacs GG, Dengjel J, Meisinger C, Sigrist SJ, and Madeo F. Endonuclease G mediates α-synuclein cytotoxicity during Parkinson’s disease EMBO J 2013, 32 (23), 3041–3054. DOI: 10.1038/emboj.2013.228

[53] Gupta VK, Scheunemann L, Eisenberg T, Mertel S, Bhukel A, Koemans TS, Kramer JM, Liu KSY, Schroeder S, Stunnenberg HG, Sinner F, Magnes C, Pieber TR, Dipt S, Fiala A, Schenck A, Schwaerzel M, Madeo F, and Sigrist SJ. Restoring polyamines protects from age-induced memory impairment in an autophagy-dependent manner Nat Neurosci 2013, 16 (10), 1453–1460. DOI: 10.1038/nn.3512


[1] Madeo F, Eisenberg T, Pietrocola F, and Kroemer G. Spermidine in health and disease. Science 2018 359(6374). DOI: 10.1126/science.aan2788

[2] Haemmerle G, Moustafa T, Woelkart G, Büttner S, Schmidt A, van de Weijer T, Hesselink M, Jaeger D, Kienesberger PC, Zierler K, Schreiber R, Eichmann T, Kolb D, Kotzbeck P, Schweiger M, Kumari M, Eder S, Schoiswohl G, Wongsiriroj N, Pollak NM, Radner FPW, Preiss-Landl K, Kolbe T, Rülicke T, Pieske B, Trauner M, Lass A, Zimmermann R, Hoefler G, Cinti S, Kershaw EE, Schrauwen P, Madeo F, Mayer B, and Zechner R. ATGL-mediated fat catabolism regulates cardiac mitochondrial function via PPAR-α and PGC-1 Nat Med 2011, 17 (9), 1076–1085. DOI: 10.1038/nm.2439

https://doi.org/10.1126/science.aan2788

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[3] Eisenberg T, Knauer H, Schauer A, Büttner S, Ruckenstuhl C, Carmona-Gutierrez D, Ring J, Schroeder S, Magnes C, Antonacci L, Fussi H, Deszcz L, Hartl R, Schraml E, Criollo A, Megalou E, Weiskopf D, Laun P, Heeren G, Breitenbach M, Grubeck-Loebenstein B, Herker E, Fahrenkrog B, Fröhlich K-U, Sinner F, Tavernarakis N, Minois N, Kroemer G, and Madeo F. Induction of autophagy by spermidine promotes longevity Nat Cell Biol 2009, 11 (11), 1305–1314. DOI: 10.1038/ncb1975

[4] Tasdemir E, Maiuri MC, Galluzzi L, Vitale I, Djavaheri-Mergny M, D’Amelio M, Criollo A, Morselli E, Zhu C, Harper F, Nannmark U, Samara C, Pinton P, Vicencio JM, Carnuccio R, Moll UM, Madeo F, Paterlini-Brechot P, Rizzuto R, Szabadkai G, Pierron G, Blomgren K, Tavernarakis N, Codogno P, Cecconi F, and Kroemer G. Regulation of autophagy by cytoplasmic p53 Nat Cell Biol 2008, 10 (6), 676–687. DOI: 10.1038/ncb1730

[5] Herker E, Jungwirth H, Lehmann KA, Maldener C, Fröhlich K-U, Wissing S, Büttner S, Fehr M, Sigrist S, and Madeo F. Chronological aging leads to apoptosis in yeast J Cell Biol 2004, 164 (4), 501–507. DOI: 10.1083/jcb.200310014

[6] Wissing S, Ludovico P, Herker E, Büttner S, Engelhardt SM, Decker T, Link A, Proksch A, Rodrigues F, Corte-Real M, Fröhlich K-U, Manns J, Candé C, Sigrist SJ, Kroemer G, and Madeo F. An AIF orthologue regulates apoptosis in yeast J Cell Biol 2004, 166 (7), 969–974. DOI: 10.1083/jcb.200404138

[7] Madeo F, Herker E, Maldener C, Wissing S, Lächelt S, Herlan M, Fehr M, Lauber K, Sigrist SJ, Wesselborg S, and Fröhlich KU. A caspase-related protease regulates apoptosis in yeast Mol Cell 2002, 9 (4), 911–917.

[8] Laun P, Pichova A, Madeo F, Fuchs J, Ellinger A, Kohlwein S, Dawes I, Fröhlich KU, and Breitenbach M. Aged mother cells of Saccharomyces cerevisiae show markers of oxidative stress and apoptosis Mol Microbiol 2001, 39(5), 1166–1173.

[9] Madeo F, Fröhlich E, Ligr M, Grey M, Sigrist SJ, Wolf DH, and Fröhlich KU. Oxygen stress: a regulator of apoptosis in yeast J Cell Biol 1999, 145 (4), 757–767.

[10] Madeo F, Fröhlich E, and Fröhlich KU. A yeast mutant showing diagnostic markers of early and late apoptosis J Cell Biol 1997, 139 (3), 729–734.

Patents

Eisenberg T, Madeo F, Stekovic S. 2016. Use of spermidine for the enhancement of mitochondrial respiration; EP16198663

Baekeland V, Buettner S, Madeo F, Winderickx J. 2014. Alpha synuclein toxicity; US2014093494 (A1)

Windericks J, Van den Berghe G, Gunst J, Vanhorebeek I, Langouche L, Eisenberg T, Madeo F, Magnes C, Sinner F. 2012. Methods and preparations for protecting critically ill patients; US2012015901 (A1)

Madeo F, Eisenberg T, Pieber T, Magnes C, Sinner F, Windericks J, Van den Berghe G, Gunst J. 2012. Methods and compositions for diagnosing medical conditions; WO2012007531 (A2); WO2012007531 (A3)

Eisenberg T, Rockenfeller P, Madeo F, Minois N, Kroemer G, Windericks J. 2010. Activators of the autophagic pathway; NL1036427 (C)

Baekeland V, Buettner S, Gerard M, Madeo F, Windericks J. 2010. Synpcleinopathies; WO2010094090 (A3); WO2010094090 (A2)

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Windericks J, Eisenberg T, Kroemer G, Madeo F, Minois N, Rockenfeller P. 2010. Activators of the autophagic pathway; WO2010081204 (A2); WO2010081204 (A3)

Froehlich KU, Madeo F. 2000. Rapid screening assay for apoptosis-modulating substances comprises determining the effects of substances on yeasts; DE19842612 (A1)

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Curriculum Vitae – Assoz. Prof. Dr. rer. nat. Monika Oberer Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, 3rd floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 5483, E-Mail: [email protected] https://molekularbiologie.uni-graz.at/en/research/areas-of-research/strukturbiologie/

Year of Birth *1973

Nationality Austrian

Orcid ID http://orcid.org/0000-0003-2525-9513




Keywords structure and function of proteins, lipid metabolism, protein-

protein/DNA/lipid interaction, lipases, helicases, NMR spectroscopy,

X-ray crystallography, biophysics; biochemistry


since 2018 Member of curriculum development group for Ph.D. studies in Natural

Sciences at the University of Graz, Austria

since 2014 Associate Professor, Institute of Molecular Biosciences (IMB), University

of Graz, Austria

2013 Habilitation, University of Graz

2011 - 2014 Assistant Professor (tenure track), IMB, University of Graz

2008 - 2011 University Assistant at the IMB, University of Graz

since 2008 Project Leader (GOLD-III, DK Molecular Enzymology, SFB Lipotox)

2006 - 2008 Co-Project Leader in ‘Genomics of Lipid-associated Disorders’ – GOLD-

II; Institute of Chemistry, University of Graz

2002 - 2006 Post-doctoral Research Fellow (2003-2005 Erwin Schrödinger

Fellowship, 2005-2006 Max-Kade Fellowship), Laboratory of Prof. G.

Wagner, BCMP, Harvard Medical School, Boston, MA, USA

Education

1998 - 2002 PhD thesis, Institute of Chemistry, University of Graz, Austria,

Graduation Promotio sub auspiciis praesidentis rei publicae Supervisor:

Prof. Dr. C. Kratky

1997 Research visit, Center of Molecular Genetics, University of California

San Diego (UCSD), La Jolla, USA

1995 - 1996 Studies in Biochemistry and Chemistry, Western Washington University,

Bellingham, USA


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1991 - 1997 Studies of Chemistry, Karl-Franzens University Graz, Austria


2018 “The crystal structure of monoacylglycerol lipase from M. tuberculosis

reveals the basis for specific inhibition”. i-next Meets Industry Workshop:

Translating Structural Biology into Biomedical Applications, Grenoble,

France

2017 “Structural Studies of Protein-protein and Protein-lipid Interactions

Determining the Fate of Fat”. The Adriatic NMR Conference, Mali Ston,

Croatia

2016 “The cap makes the difference: Structural evidence for conserved cap

architecture and conformational plasticity in monoacylglycerol lipases”.

8th Conference Chemistry towards Biology, Brno, Czech Republic

2014 “Structural Biology in Lipid Metabolism”. Young Investigators in Lipid

Science, Düsseldorf, Germany

2013 „Structural Investigations in Lipid Metabolism“. X. H.P. Kaufmann-Tage,

German Society for Fat Science, Heidelberg, Germany

Top 5 Honors ▪ Awards

2015 Promotion Award for Scientific Achievements (Förderungspreis)

sponsored by the State of Styria, Austria

2015 Journal of Structural and Functional Genomics Poster Award, ICSG,

Rehovot, Israel

2013 Kardinal-Innitzer-Förderungspreis (Cardinal-Innitzer Promotion Award),

Kardinal-Innitzer-Studienfonds, Vienna

2008 Award for Excellent Scientific Achievements, University of Graz

2002 Würdigungspreis (Award of Academic Excellence), Austrian Federal

Ministry of Science


since 2014 Reviewer Fonds Wetenschappelijk Onderzoek - Vlaanderen, FWO

since 2011 Editorial board member PLoSOne

since 2010 Reviewer of peer-reviewed Journals (Cell Metabolism, e-Life, BBA, JBC,

Biochemistry, PLoSOne, JLR, Journal of Structural Biology, etc)

since 2010 Scientific and organizing committees (e.g. Austrian Bio-NMR Meeting,

Intern. Graz Symposium on Lipid and Membrane Biology, Graz, Austria)

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18

since 2008 Member of the ÖGMBT (Austrian Association of Molecular Life Sciences

and Biotechnology) and FEBS (Federation of European Biochemical

Societies)



Funding Org.

Project Number


Funding Period

Foundation Leducq

12CVD04

Transatlantic Network on Triglycerides — Triglyceride Metabolism in Obesity and

Cardiovascular Disease

191 2013-2018

The Max Planck Society

Characterization of the

mitochondrial fission factor 107 2017-2018

FWF P24857 Structure, dynamics, and substrate specificity of monoglyceride lipases

348 2012-2017

FWF/ University support

W9 DK Molecular Enzymology, 3rd and

4th funding period 559 2011-2019


F3016 SFB Lipotox, Subproject P16;

Priority Research Program 2nd and 3rd Funding Period

577 2011-2017

Top 5 International Collaborations

Haribabu ARTHANARI, Dana-Faber Cancer Institute, Boston, USA

Irina CORNACIU and Jose A. MÁRQUEZ, Grenoble Outstation, European Molecular Biology

Laboratory, Grenoble, France

Ingrid DREVENY, School of Pharmacy, University of Nottingham, United Kingdom

Gerhard WAGNER, Biological Chemistry and Molecular Pharmacology, Harvard Medical

School, Boston, USA

Stephen G. YOUNG, Dept. of Medicine/Div. of Cardiology, University of California Los

Angeles (UCLA), USA


Ingrid DREVENY, School of Pharmacy, University of Nottingham, United Kingdom: Surface

plasmon resonance, small volume PEAQ-ITC

Irina CORNACIU and Jose A. MÁRQUEZ, Grenoble Outstation, European Molecular Biology

Laboratory, Grenoble, France: High-Throughput Crystalization (HTX) Laboratory

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[1] Riegler-Berket L., Leitmeier A., Aschauer P., Dreveny I., Oberer M. Identification of lipases with activity towards monoacylglycerol by criterion of conserved cap architectures. Biochim Biophys Acta 2018, 1863(7), 679-687. DOI: 10.1016/j.bbalip.2018.03.009.

[2] Padmanabha Das K.M., Wechselberger L., Liziczai M., De la Rosa Rodriguez M., Grabner G.F., Heier C., Viertlmayr R., Radler C., Lichtenegger J., Zimmermann R., Borst J.W., Zechner R., Kersten S., Oberer M. Hypoxia-inducible lipid droplet-associated protein inhibits adipose triglyceride lipase. J Lipid Res 2018, 59, 531-541. DOI: 10.1194/jlr.M082388.

[3] Cerk I.K., Wechselberger L., Oberer M. Adipose Triglyceride Lipase Regulation: An Overview. Curr Protein Pept Sci 2018; 19, 221-233. DOI: 10.2174/1389203718666170918160110.

[4] Thul P.J., Tschapalda K., Kolkhof P., Thiam A.R., Oberer M., Beller M. Targeting of the Drosophila protein CG2254/Ldsdh1 to a subset of lipid droplets. J Cell Sci 2017, 130, 3141-3157. DOI: 10.1242/jcs.199661.

[5] Hromic-Jahjefendic A., Jajcanin Jozic N., Kazazic S., Grabar Branilovic M., Karacic Z., Schrittwieser J.H., Das K.M.P., Tomin M., Oberer M., Gruber K., Abramic M., Tomic S. A novel Porphyromonas gingivalis enzyme: An atypical dipeptidyl peptidase III with an ARM repeat domain. PLoS One 2017, 12:e0188915 DOI:10.1371/journal.pone.0188915

[6] Heier, C., Taschler, U., Radulovic, M., Aschauer, P., Eichmann, T.O., Grond, S., Wolinski, H., Oberer, M., Zechner, R., Kohlwein, S.D., Zimmermann, R. Monoacylglycerol Lipases Act as Evolutionarily Conserved Regulators of Non-oxidative Ethanol Metabolism J Biol Chem 2016, 291 (22), 11865-11875. DOI: 10.1074/jbc.M115.705541

[7] Fong, L.G., Young, S.G., Beigneux, A.P., Bensadoun, A., Oberer, M., Jiang, H., Ploug, M. GPIHBP1 and Plasma Triglyceride Metabolism Trends Endocrinol Metab 2016, 27 (7), 455-469. DOI: 10.1016/j.tem.2016.04.013

[8] Aschauer, P., Rengachari, S., Lichtenegger, J., Schittmayer, M., Das, K.M., Mayer, N., Breinbauer, R., Birner-Gruenberger, R., Gruber, C.C., Zimmermann, R., Gruber, K., Oberer, M. Crystal structure of the Saccharomyces cerevisiae monoglyceride lipase Yju3p Biochim Biophys Acta 2016, 1861 (5), 462-470. DOI: 10.1016/j.bbalip.2016.02.005

[9] Sahu-Osen, A., Montero-Moran, G., Schittmayer, M., Fritz, K., Dinh, A., Chang, Y.F., McMahon, D., Boeszoermenyi, A., Cornaciu, I., Russell, D., Oberer, M., Carman, G.M., Birner-Gruenberger, R., Brasaemle, D.L. CGI-58/ABHD5 is phosphorylated on Ser239 by protein kinase A: control of subcellular localization J Lipid Res 2015, 56 (1), 109-121. DOI: 10.1194/jlr.M055004

[10] Rengachari, S., Aschauer, P., Sturm, C., Oberer, M. Purification, crystallization and preliminary X-ray diffraction analysis of a soluble variant of the monoglyceride lipase Yju3p from the yeast Saccharomyces cerevisiae Acta Crystallogr F Struct Biol Commun 2015, 71 (Pt 2), 243-246. DOI: 10.1107/S2053230X15001557

[11] Hofer, P., Boeszoermenyi, A., Jaeger, D., Feiler, U., Arthanari, H., Mayer, N., Zehender, F., Rechberger, G., Oberer, M., Zimmermann, R., Lass, A., Haemmerle, G., Breinbauer, R., Zechner, R., Preiss-Landl, K. Fatty Acid-binding Proteins Interact with Comparative Gene Identification-58 Linking Lipolysis with Lipid Ligand Shuttling J Biol Chem 2015, 290 (30), 18438-18453. DOI: 10.1074/jbc.M114.628958

[12] Heier, C., Radner, F.P., Moustafa, T., Schreiber, R., Grond, S., Eichmann, T.O., Schweiger, M., Schmidt, A., Cerk, I.K., Oberer, M., Theussl, H.C., Wojciechowski, J., Penninger, J.M., Zimmermann, R., Zechner, R. G0/G1 Switch Gene 2 Regulates Cardiac Lipolysis J Biol Chem 2015, 290 (43), 26141-26150. DOI: 10.1074/jbc.M115.671842

E3: M. Oberer

20

[13] Boeszoermenyi, A., Nagy, H.M., Arthanari, H., Pillip, C.J., Lindermuth, H., Luna, R.E., Wagner, G., Zechner, R., Zangger, K., Oberer, M. Structure of a CGI-58 Motif Provides the Molecular Basis of Lipid Droplet Anchoring J Biol Chem 2015, 290 (44), 26361-26372. DOI: 10.1074/jbc.M115.682203

[14] Beigneux, A.P., Fong, L.G., Bensadoun, A., Davies, B.S., Oberer, M., Gardsvoll, H., Ploug, M., Young, S.G. GPIHBP1 missense mutations often cause multimerization of GPIHBP1 and thereby prevent lipoprotein lipase binding Circ Res 2015, 116 (4), 624-632. DOI: 10.1161/CIRCRESAHA.116.305085

[15] Adeyo, O., Oberer, M., Ploug, M., Fong, L.G., Young, S.G., Beigneux, A.P. Heterogeneity in the properties of mutant secreted lymphocyte antigen 6/urokinase receptor-related protein 1 (SLURP1) in Mal de Meleda Br J Dermatol 2015, 173 (4), 1066-1069. DOI: 10.1111/bjd.13868

[16] Steinkellner, G., Gruber, C.C., Pavkov-Keller, T., Binter, A., Steiner, K., Winkler, C., Lyskowski, A., Schwamberger, O., Oberer, M., Schwab, H., Faber, K., Macheroux, P., Gruber, K. Identification of promiscuous ene-reductase activity by mining structural databases using active site constellations Nat Commun 2014, 5, 4150. DOI: 10.1038/ncomms5150

[17] Nagy, H.M., Paar, M., Heier, C., Moustafa, T., Hofer, P., Haemmerle, G., Lass, A., Zechner, R., Oberer, M., Zimmermann, R. Adipose triglyceride lipase activity is inhibited by long-chain acyl-coenzyme A Biochim Biophys Acta 2014, 1841 (4), 588-594. DOI: 10.1016/j.bbalip.2014.01.005

[18] Harper, S., Gratton, H.E., Cornaciu, I., Oberer, M., Scott, D.J., Emsley, J., Dreveny, I. Structure and catalytic regulatory function of ubiquitin specific protease 11 N-terminal and ubiquitin-like domains Biochemistry 2014, 53 (18), 2966-2978. DOI: 10.1021/bi500116x

[19] Cerk, I.K., Salzburger, B., Boeszoermenyi, A., Heier, C., Pillip, C., Romauch, M., Schweiger, M., Cornaciu, I., Lass, A., Zimmermann, R., Zechner, R., Oberer, M. A peptide derived from G0/G1 switch gene 2 acts as noncompetitive inhibitor of adipose triglyceride lipase J Biol Chem 2014, 289 (47), 32559-32570. DOI: 10.1074/jbc.M114.602599

[20] Boeszoermenyi, A., Schmidt, J.C., Cheeseman, I.M., Oberer, M., Wagner, G., Arthanari, H. Resonance assignments of the microtubule-binding domain of the C. elegans spindle and kinetochore-associated protein 1 Biomol NMR Assign 2014, 8 (2), 275-278. DOI: 10.1007/s12104-013-9499-x

[21] Thiel, K., Heier, C., Haberl, V., Thul, P.J., Oberer, M., Lass, A., Jackle, H., Beller, M. The evolutionarily conserved protein CG9186 is associated with lipid droplets, required for their positioning and for fat storage J Cell Sci 2013, 126 (Pt 10), 2198-2212. DOI: 10.1242/jcs.120493

[22] Rengachari, S., Aschauer, P., Schittmayer, M., Mayer, N., Gruber, K., Breinbauer, R., Birner-Gruenberger, R., Dreveny, I., Oberer, M. Conformational plasticity and ligand binding of bacterial monoacylglycerol lipase J Biol Chem 2013, 288 (43), 31093-31104. DOI: 10.1074/jbc.M113.491415

[23] Schweiger, M., Paar, M., Eder, C., Brandis, J., Moser, E., Gorkiewicz, G., Grond, S.,

Radner, F.P., Cerk, I., Cornaciu, I., Oberer, M., Kersten, S., Zechner, R., Zimmermann,

R., Lass, A. G0/G1 switch gene-2 regulates human adipocyte lipolysis by affecting

activity and localization of adipose triglyceride lipase J Lipid Res 2012, 53 (11), 2307-

2317. DOI: 10.1194/jlr.M027409

E3: M. Oberer

21


[1] Riegler-Berket L., Leitmeier A., Aschauer P., Dreveny I., Oberer M. Identification of lipases with activity towards monoacylglycerol by criterion of conserved cap architectures. Biochim Biophys Acta 2018, 1863(7), 679-687. DOI: 10.1016/j.bbalip.2018.03.009..

[2] Padmanabha Das K.M., Wechselberger L., Liziczai M., De la Rosa Rodriguez M., Grabner G.F., Heier C., Viertlmayr R., Radler C., Lichtenegger J., Zimmermann R., Borst J.W., Zechner R., Kersten S., Oberer M. Hypoxia-inducible lipid droplet-associated protein inhibits adipose triglyceride lipase. J Lipid Res 2018; 59, 531-541. DOI: 10.1194/jlr.M082388.

[3] Aschauer, P., Rengachari, S., Lichtenegger, J., Schittmayer, M., Das, K.M., Mayer, N., Breinbauer, R., Birner-Gruenberger, R., Gruber, C.C., Zimmermann, R., Gruber, K., Oberer, M. Crystal structure of the Saccharomyces cerevisiae monoglyceride lipase Yju3p Biochim Biophys Acta 2016, 1861 (5), 462-470. DOI: 10.1016/j.bbalip.2016.02.005

[4] Boeszoermenyi, A., Nagy, H.M., Arthanari, H., Pillip, C.J., Lindermuth, H., Luna, R.E., Wagner, G., Zechner, R., Zangger, K., Oberer, M. Structure of a CGI-58 Motif Provides the Molecular Basis of Lipid Droplet Anchoring J Biol Chem 2015, 290 (44), 26361-26372. DOI: 10.1074/jbc.M115.682203

[5] Cerk, I.K., Salzburger, B., Boeszoermenyi, A., Heier, C., Pillip, C., Romauch, M., Schweiger, M., Cornaciu, I., Lass, A., Zimmermann, R., Zechner, R., Oberer, M. A peptide derived from G0/G1 switch gene 2 acts as noncompetitive inhibitor of adipose triglyceride lipase J Biol Chem 2014, 289 (47), 32559-32570. DOI: 10.1074/jbc.M114.602599

[6] Rengachari, S., Aschauer, P., Schittmayer, M., Mayer, N., Gruber, K., Breinbauer, R., Birner-Gruenberger, R., Dreveny, I., Oberer, M. Conformational plasticity and ligand binding of bacterial monoacylglycerol lipase J Biol Chem 2013, 288 (43), 31093-31104. DOI: 10.1074/jbc.M113.491415

[7] Gruber, A., Cornaciu, I., Lass, A., Schweiger, M., Poeschl, M., Eder, C., Kumari, M., Schoiswohl, G., Wolinski, H., Kohlwein, S.D., Zechner, R., Zimmermann, R., Oberer, M. The N-terminal region of comparative gene identification-58 (CGI-58) is important for lipid droplet binding and activation of adipose triglyceride lipase J Biol Chem 2010, 285 (16), 12289-12298. DOI: 10.1074/jbc.M109.064469

[8] Marintchev, A., Edmonds, K.A., Marintcheva, B., Hendrickson, E., Oberer, M., Suzuki, C., Herdy, B., Sonenberg, N., Wagner, G. Topology and regulation of the human eIF4A/4G/4H helicase complex in translation initiation Cell 2009, 136 (3), 447-460. DOI: 10.1016/j.cell.2009.01.014

[9] Bordeleau, M.E., Mori, A., Oberer, M., Lindqvist, L., Chard, L.S., Higa, T., Belsham, G.J., Wagner, G., Tanaka, J., Pelletier, J. Functional characterization of IRESes by an inhibitor of the RNA helicase eIF4A Nat Chem Biol 2006, 2 (4), 213-220. DOI: 10.1038/nchembio776

[10] Oberer, M., Marintchev, A., Wagner, G. Structural basis for the enhancement of eIF4A helicase activity by eIF4G Genes Dev 2005, 19 (18), 2212-2223. DOI: 10.1101/gad.1335305

E4: B. Pertschy

22

Curriculum Vitae – Priv.Doz. Dr. rer. nat. Brigitte Pertschy Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, 1st floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 1518, E-Mail: [email protected] https://molekularbiologie.uni-graz.at/en/bergler-pertschy-groups

Year of Birth *1976


Orcid ID http:// orcid.org/0000-0003-3558-0191




Keywords ribosome biogenesis, 40S subunit maturation, RNA-protein complexes,

RNA helicases, nuclear transport, ribosomal RNA metabolism,

ribosomal proteins, dedicated chaperones, yeast


2014 Habilitation in Biochemistry and Molecular Biology at the University of

Graz

since 2009 Independent postdoctoral researcher and group leader (own position

funded by the FWF via a Hertha Firnberg fellowship, an Elise Richter

fellowship and stand alone projects) at the Institute for Molecular

Biosciences, University of Graz.

2005 - 2009 Postdoctoral researcher in the group of Ed Hurt, BZH Heidelberg

Education

2001 - 2005 PhD training, Institute of Microbiology, University of Graz, Austria,

Supervisor: Prof. Dr. G. Högenauer

2000 Exchange semester at the University of Queensland, Brisbane,

Australia

1995 - 2001 Studies in Microbiology at the University of Graz, Austria


E4: B. Pertschy

23


2017 “Coordination of distant 40S ribosomal subunit maturation events", The

Biology of RNA protein complexes conference, Regensburg, Germany

2015 “The ribosome assembly path of Rps3", Ribosome Synthesis Meeting,

Brussels, Belgium

2013 „The interaction of ribosomal protein S3 with ankyrin repeat proteins in

ribosome biogenesis and extraribosomal pathways", RNP Biogenesis

Meeting, Regensburg, Germany

2009 “RNA helicase Prp43 and its co-factor Pfa1 promote 20S to 18S rRNA

processing catalyzed by the D-site specific endonuclease Nob1",

Ribosome Synthesis Meeting, Regensburg, Germany

2004 “Diazaborine treatment of yeast cells inhibits maturation of the 60S

ribosomal subunit”, Young Investigator Award lecture at the ÖGGGT

Meeting, Innsbruck, Austria


2013 Elise Richter Senior Postdoctoral Fellowship, funded by the FWF

2009 Hertha Firnberg Postdoctoral Fellowship, funded by the FWF

2006 Erwin Schrödinger Fellowship Abroad, funded by the FWF

2004 ÖGGGT Research Prize for Young Scientists, awarded by the Austrian

Society of Genetics and Gene Technology (ÖGGGT)


since 2016 Editorial board member of Microbial Cell

since 2014 Reviewer of peer-reviewed Journals (Nature Communications, Nucleic

Acids Research, Plant Cell, RNA, etc)

since 2012 Member of the RNA society

since 2010 Member of the ÖGMBT (Austrian Association of Molecular Life Sciences

and Biotechnology)

E4: B. Pertschy

24



Funding Org.

Project Number


Funding Period

FWF P28874 Restructuring Events in 40S

maturation 342 2016-2019

FWF P27996 The ribosome assembly path of

Rps3 244 2015-2018

University of Graz

AVO-164700404

Incentive funding for the investigation of ribosomal protein

chaperones 80 2015-2017

FWF P23682 A specific chaperone for ribosomal

protein S3 300 2012-2016

FWF P23883 Role of Rps15 in 40S assembly 150 2011-2015


Dieter KRESSLER, Unit of Biochemistry, University of Fribourg, Switzerland

Nicolas LEULLIOT, CNRS, Université Paris Descartes, France

Celia PLISSON-CHASTANG, Centre de Biologie Integrative, University of Toulouse, France

Sebastién FERREIRA-CERCA, Institute of Biochemistry, Genetics and Microbiology,

University of Regensburg, Germany

Johannes HAYBÄCK, Institute for Pathology, University of Magdeburg


Nicolas LEULLIOT, CNRS, Université Paris Descartes, France: structure of the Prp43-

Tma23 complex

Dieter KRESSLER, Unit of Biochemistry, University of Fribourg, Switzerland: investigation of

genetic links between Tma23 and ribosome maturation factors

E4: B. Pertschy

25


[1] Zisser, G., Ohmayer, U., Mauerhofer, C., Mitterer, V., Klein, I., Rechberger, G.N., Wolinski, H., Prattes, M., Pertschy, B., Milkereit, P., Bergler, H. Viewing pre-60S maturation at a minute's timescale. Nucleic Acids Res 2018, 46(6):3140-3151. DOI: 10.1093/nar/gkx1293.

[2] Sprenger, H., Kienesberger, S., Pertschy, B., Pöltl, L., Konrad, B., Bhutada, P., Vorkapic, D., Atzmüller, D., Feist, F., Högenauer, C., Gorkiewicz, G., Zechner, E.L. Fic proteins of Campylobacter fetus subsp. venerealis form a network of functional toxin–antitoxin systems. Front Microbiol 2017, 8:1965. DOI: 10.3389/fmicb.2017.01965

[3] Golob-Schwarzl, N., Schweiger, C., Koller, C., Krassnig, S., Gogg-Kamerer, M., Gantenbein, N., Toeglhofer, A.M., Wodlej, C., Bergler, H., Pertschy, B., Uranitsch, S., Holter, M., El-Heliebi, A., Fuchs, J., Punschart, A., Stiegler, P., Keil, M., Hoffmann, J., Henderson, D., Lehrach, H., Reinhard, C., Regenbrecht, C., Schicho, R., Fickert, P., Lax, S., Haybaeck, J. Separation of low and high grade colon and rectum carcinoma by eukaryotic translation initiation factors 1, 5 and 6. Oncotarget 2017, 8(60):101224-101243. DOI: 10.18632/oncotarget.20642

[4] Prattes, M., Loibl, M., Zisser, G., Luschnig, D., Kappel, L., Rössler, I., Grassegger, M., Hromic, A., Krieger, E., Gruber, K., Pertschy, B., Bergler, H. A conserved inter-domain communication mechanism regulates the ATPase activity of the AAA-protein Drg1. Sci Rep 2017, 7, 44751. DOI: 10.1038/srep44751.

[5] Pillet, B., Mitterer, V., Kressler, D., Pertschy, B. Hold on to your friends: Dedicated chaperones of ribosomal proteins: Dedicated chaperones mediate the safe transfer of ribosomal proteins to their site of pre-ribosome incorporation. Bioessays 2017, 39(1):1-12. DOI: 10.1002/bies.201600153.

[6] Mitterer, V., Gantenbein, N., Birner-Gruenberger, R., Murat, G., Bergler, H., Kressler, D., Pertschy, B. Nuclear import of dimerized ribosomal protein Rps3 in complex with its chaperone Yar1. Sci Rep 2016, 6:36714. DOI: 10.1038/srep36714.

[7] Mitterer, V., Murat, G., Réty, S., Blaud, M., Delbos, L., Stanborough, T., Bergler, H., Leulliot, N., Kressler, D., Pertschy, B. Sequential domain assembly of ribosomal protein S3 drives 40S subunit communication. Nat Commun 2016, 7, 10336. DOI: 10.1038/ncomms10336.

[8] Stanborough, T., Niederhauser, J., Koch, B., Bergler, H., Pertschy, B. Ribosomal protein S3 interacts with the NF-kappaB inhibitor IkappaBalpha FEBS Lett 2014, 588 (5), 659-664. DOI:10.1016/j.febslet.2013.12.034

[9] Loibl, M., Klein, I., Prattes, M., Schmidt, C., Kappel, L., Zisser, G., Gungl, A., Krieger, E., Pertschy, B., Bergler, H. The drug diazaborine blocks ribosome biogenesis by inhibiting the AAA-ATPase Drg1 J Biol Chem 2014, 289 (7), 3913-3922. DOI:10.1074/jbc.M113.536110

[10] Reinthaler, F.F., Galler, H., Feierl, G., Haas, D., Leitner, E., Mascher, F., Melkes, A., Posch, J., Pertschy, B., Winter, I., Himmel, W., Marth, E., Zarfel, G. Resistance patterns of Escherichia coli isolated from sewage sludge in comparison with those isolated from human patients in 2000 and 2009 J Water Health 2013, 11 (1), 13-20. DOI:10.2166/wh.2012.207

[11] Zarfel, G., Galler, H., Feierl, G., Haas, D., Kittinger, C., Leitner, E., Grisold, A.J., Mascher, F., Posch, J., Pertschy, B., Marth, E., Reinthaler, F.F. Comparison of extended-spectrum-beta-lactamase (ESBL) carrying Escherichia coli from sewage sludge and human urinary tract infection Environ Pollut 2013, 173, 192-199. DOI:10.1016/j.envpol.2012.09.019

https://www.ncbi.nlm.nih.gov/pubmed/29294095


http://www.frontiersin.org/people/u/281585






https://doi.org/10.3389/fmicb.2017.01965

http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path%5b%5d=20642

http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path%5b%5d=20642

https://doi.org/10.18632/oncotarget.20642

E4: B. Pertschy

26

[12] Kappel, L., Loibl, M., Zisser, G., Klein, I., Fruhmann, G., Gruber, C., Unterweger, S., Rechberger, G., Pertschy, B., Bergler, H. Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation J Cell Biol 2012, 199 (5), 771-782. DOI:10.1083/jcb.201205021

[13] Koch, B., Mitterer, V., Niederhauser, J., Stanborough, T., Murat, G., Rechberger, G., Bergler, H., Kressler, D., Pertschy, B. Yar1 protects the ribosomal protein Rps3 from aggregation J Biol Chem 2012, 287 (26), 21806-21815. DOI:10.1074/jbc.M112.365791


[1] Sprenger, H., Kienesberger, S., Pertschy, B., Pöltl, L., Konrad, B., Bhutada, P., Vorkapic, D., Atzmüller, D., Feist, F., Högenauer, C., Gorkiewicz, G., Zechner, E.L. Fic proteins of Campylobacter fetus subsp. venerealis form a network of functional toxin–antitoxin systems. Front Microbiol 2017, 8:1965. DOI: 10.3389/fmicb.2017.01965

[2] Pillet, B., Mitterer, V., Kressler, D., Pertschy, B. Hold on to your friends: Dedicated chaperones of ribosomal proteins: Dedicated chaperones mediate the safe transfer of ribosomal proteins to their site of pre-ribosome incorporation. Bioessays 2017, 39(1):1-12. DOI: 10.1002/bies.201600153.

[3] Mitterer, V., Gantenbein, N., Birner-Gruenberger, R., Murat, G., Bergler, H., Kressler, D., Pertschy, B. Nuclear import of dimerized ribosomal protein Rps3 in complex with its chaperone Yar1. Sci Rep 2016, 6:36714. DOI: 10.1038/srep36714.

[4] Mitterer, V., Murat, G., Réty, S., Blaud, M., Delbos, L., Stanborough, T., Bergler, H., Leulliot, N., Kressler, D., Pertschy, B. Sequential domain assembly of ribosomal protein S3 drives 40S subunit communication. Nat Commun 2016, 7, 10336. DOI: 10.1038/ncomms10336.

[5] Stanborough, T., Niederhauser, J., Koch, B., Bergler, H., Pertschy, B. Ribosomal protein S3 interacts with the NF-kappaB inhibitor IkappaBalpha FEBS Lett 2014, 588 (5), 659-664. DOI:10.1016/j.febslet.2013.12.034

[6] Koch, B., Mitterer, V., Niederhauser, J., Stanborough, T., Murat, G., Rechberger, G., Bergler, H., Kressler, D., Pertschy, B. Yar1 protects the ribosomal protein Rps3 from aggregation J Biol Chem 2012, 287 (26), 21806-21815. DOI:10.1074/jbc.M112.365791

[7] Pertschy, B., Schneider, C., Gnädig, M., Schafer, T., Tollervey, D., Hurt, E. RNA helicase Prp43 and its co-factor Pfa1 promote 20 to 18 S rRNA processing catalyzed by the endonuclease Nob1 J Biol Chem 2009, 284 (50), 35079-35091. DOI:10.1074/jbc.M109.040774

[8] Ulbrich, C., Diepholz, M., Bassler, J., Kressler, D., Pertschy, B., Galani, K., Böttcher, B., Hurt, E. Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits Cell 2009, 138 (5), 911-922. DOI:10.1016/j.cell.2009.06.045

[9] Pertschy, B., Saveanu, C., Zisser, G., Lebreton, A., Tengg, M., Jacquier, A., Liebminger, E., Nobis, B., Kappel, L., van der Klei, I., Högenauer, G., Fromont-Racine, M., Bergler, H. Cytoplasmic recycling of 60S preribosomal factors depends on the AAA protein Drg1 Mol Cell Biol 2007, 27 (19), 6581-6592. DOI:10.1128/MCB.00668-07

[10] Pertschy, B., Zisser, G., Schein, H., Köffel, R., Rauch, G., Grillitsch, K., Morgenstern, C., Durchschlag, M., Högenauer, G., Bergler, H. Diazaborine treatment of yeast cells inhibits maturation of the 60S ribosomal subunit Mol Cell Biol 2004, 24 (14), 6476-6487. DOI:10.1128/MCB.24.14.6476-6487.2004







https://doi.org/10.3389/fmicb.2017.01965

E5: S. Schild

27

Curriculum Vitae – Assoz. Prof. Dr. rer. nat. Stefan Schild Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, 1st floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 1970, E-Mail: [email protected] https://molekularbiologie.uni-graz.at/index.php?id=26468

Year of Birth *1976

Nationality German



h-Index 20 (Scopus, April, 2018)


Keywords bacterial pathogens, virulence, biofilm, transmission, nutrient aquisition,

nucleases, surface structures, colonization


since 2011 Associate professor, Institute of Molecular Biosciences, University of

Graz, Austria

2011 Habilitation in “Microbiology”

2010 - 2011 Assistant professor, Institute of Molecular Biosciences, University of

Graz, Austria

2008 - 2010 University Assistant, Institute of Molecular Biosciences, University of

Graz, Austria

2005 - 2008 Howard Hughes Medical Institute (HHMI) research associate in the lab

of Dr. Andrew Camilli, Department of Molecular Biology & Microbiology,

Tufts University, Boston, MA

Education

2002 - 2005 PhD thesis (very good)

University of Würzburg, Germany, Supervisor: Prof. Dr. J. Reidl

1996 - 2002 Study in Biology (with distinction), University of Würzburg, Germany

Supervisor diploma thesis: Prof. Dr. J. Hacker


2017 “Friend or Foe: Bacterial membrane vesicles in host-pathogen

interactions“. University of Münster. May 2017

2015 “Bacterial Pathogenesis: Adaptation strategies, surface structures and

therapeutic interventions“. University of Hannover. October 2015


E5: S. Schild

28

2015 "From outer membrane vesicles to nanoparticles: Therapeutic

approaches to fight bacterial infections". University of Stuttgart

Hohenheim. May 2015

2013 “OMVs - Vaccine against Pasteurellaceae”. Biovaria Munich. June 2013

2011 “Extracellular DNA and Vibrio cholerae biofilm dynamics“ Microbial

pathogenesis and Host response. Cold Spring Harbor Laboratory.

September 2011


2014 Innovation award, Science Park Graz

2011 Travel award from the Land Steiermark

2009 Publication award from the Heinrich-Jörg Stiftung

2002 – 2005 PhD fellowship from the “Studienstiftung des deutschen Volkes”


since 2017 Editorial board member "International Journal of Medical Microbiology"

since 2017 Editorial board member "Microbial Cell"

since 2013 member of the ethic commission for animal research, Institute of

Molecular Biosciences, University of Graz, Austria

since 2011 Reviewer of peer-reviewed Journals (PLoS Pathogens, e-Life, Infection

and Immunity, Journal of Bacteriology, Traffic, PLoSOne, JLR, etc)

since 2008 Coordinator of animal research for the Dept. of Microbiology, Institute of

Molecular Biosciences, University of Graz, Austria

E5: S. Schild

29



Funding Org.

Project Number

Research Topic Amount funded [kEuro]

Funding Period

FWF P 27654 Characterization of Vibrio cholerae

biofilms 329 2018-2020

BioTechMed/ University Support

-

Immunomodulatory and Antiproliferative Bioactivities of the Bacterial Secretome

in Gastrointestinal Disease and Therapy (Coordinator & PI)

205 (430 for

consortium) 2017-2019


W9 DK Molecular Enzymology 3rd and 4th funding period

559 2011-2019

FWF P 25691 Outer membrane vesicles of

enteric pathogens 306 2013-2018

FWF P 22986 Gene regulation in dynamic

biofilms 282 2011-2015


Mario F. FELDMAN, Washington School of Medicine, St. Louis, USA

Julia FRITZ-STEUBER, Department of Cellular Microbiology, University of Stuttgart

Hohenheim, Germany

Meta KUEHN, Department of Biochemistry, Duke University, Durham, USA

Alexander SCHMIDT, Inst. of Infectiology, University of Münster, Germany

Andrew CAMILLI, Department of Microbiology, Tufts University, Boston, USA


Julia FRITZ-STEUBER, Department of Cellular Microbiology, University of Stuttgart

Hohenheim, Germany: Expert for membrane-associated enyzmes of bacteria

C. URBAN, Department of Clinical Microbiology, University of UMEA, Sweden: Impact of Xds

inhibition on neutrophil extracellular trap escape

G. WAKSMAN, UCL London, UK: Expert for structural analysis of membrane proteins

E5: S. Schild

30


[1] Cakar F, Zingl FG, Moisi M, Reidl J, Schild S. In vivo repressed genes of Vibrio cholerae reveal inverse requirements of an H+/Cl- transporter along the gastrointestinal passage. Proc Natl Acad Sci U S A 2018, 115(10):E2376-E2385. DOI: 10.1073/pnas.1716973115

[2] Wurm P., Tutz S., Mutsam B., Vorkapic D., Heyne B., Grabner C., Kleewein K., Halscheidt A., Schild, S., Reidl, J. Stringent factor and proteolysis control of sigma factor RpoS expression in Vibrio cholerae. Int J Med Microbiol 2017, DOI: 10.1016/j.ijmm.2017.01.006

[3] Lichtenegger, S., Bina, I., Durakovic, S., Glaser, P., Tutz, S., Schild, S., Reidl, J. Serum resistance and phase variation of a nasopharyngeal non-typeable Haemophilus influenzae isolate. Int J Med Microbiol 2017, DOI: 0.1016/j.ijmm.2017.01.005

[4] Vorkapic, D., Pressler, K., Schild, S. Multifaceted roles of extracellular DNA in bacterial physiology Current Genet 2016, 62 (1), 71-79. DOI: 10.1007/s00294-015-0514-x

[5] Roier S, Zingl FG, Cakar F, Schild, S. Bacterial outer membrane vesicle biogenesis: a new mechanism and its implications. Microbial Cell 2016, 3, 257-259. DOI: 10.15698/mic2016.06.508

[6] Roier, S., Zingl, F.G., Cakar, F., Durakovic, S., Kohl, P., Eichmann, T.O., Klug, L., Gadermaier, B., Weinzerl, K., Prassl, R., Lass, A., Daum, G., Reidl, J., Feldman, M.F., Schild, S. A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria Nat Commun 2016, 7, 10515. DOI: 10.1038/ncomms10515

[7] Pressler, K., Vorkapic, D., Lichtenegger, S., Malli, G., Barilich, B.P., Cakar, F., Zingl, F.G., Reidl, J., Schild, S. AAA+ proteases and their role in distinct stages along the Vibrio cholerae lifecycle Int J Med Microbiol 2016, 306 (6), 452-462. DOI: 10.1016/j.ijmm.2016.05.013

[8] Gumpenberger, T., Vorkapic, D., Zingl, F.G., Pressler, K., Lackner, S., Seper, A., Reidl, J., Schild, S. Nucleoside uptake in Vibrio cholerae and its role in the transition fitness from host to environment Mol Microbiol 2016, 99 (3), 470-483. DOI: 10.1111/mmi.13143

[9] Leitner DR, Zingl FG, Schild S. A Glimpse on Outer Membrane Vesicles as Vaccine Candidates. J Vaccines Vaccin 2015 6:293. DOI: 10.4172/2157-7560.1000293.

[10] Salem, W., Leitner, D.R., Zingl, F.G., Schratter, G., Prassl, R., Goessler, W., Reidl, J., Schild, S. Antibacterial activity of silver and zinc nanoparticles against Vibrio cholerae and enterotoxic Escherichia coli Int J Med Microbiol 2015, 305 (1), 85-95. DOI: 10.1016/j.ijmm.2014.11.005

[11] Roier, S., Blume, T., Klug, L., Wagner, G.E., Elhenawy, W., Zangger, K., Prassl, R., Reidl, J., Daum, G., Feldman, M.F., Schild, S. A basis for vaccine development: Comparative characterization of Haemophilus influenzae outer membrane vesicles Int J Med Microbiol 2015, 305 (3), 298-309. DOI: 10.1016/j.ijmm.2014.12.005

[12] Leitner, D.R., Lichtenegger, S., Temel, P., Zingl, F.G., Ratzberger, D., Roier, S., Schild-Prufert, K., Feichter, S., Reidl, J., Schild, S. A combined vaccine approach against Vibrio cholerae and ETEC based on outer membrane vesicles Front Microbiol 2015, 6, 823. DOI: 10.3389/fmicb.2015.00823

[13] Seper, A., Pressler, K., Kariisa, A., Haid, A.G., Roier, S., Leitner, D.R., Reidl, J., Tamayo, R., Schild, S. Identification of genes induced in Vibrio cholerae in a dynamic biofilm system Int J Med Microbiol 2014, 304 (5-6), 749-763. DOI: 10.1016/j.ijmm.2014.05.011

[14] Schneditz, G., Rentner, J., Roier, S., Pletz, J., Herzog, K.A., Bucker, R., Troeger, H., Schild, S., Weber, H., Breinbauer, R., Gorkiewicz, G., Hogenauer, C., Zechner, E.L.

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Enterotoxicity of a nonribosomal peptide causes antibiotic-associated colitis Proc Natl Acad Sci U S A 2014, 111 (36), 13181-13186. DOI: 10.1073/pnas.1403274111

[15] Lichtenegger, S., Bina, I., Roier, S., Bauernfeind, S., Keidel, K., Schild, S., Anthony, M., Reidl, J. Characterization of lactate utilization and its implication on the physiology of Haemophilus influenzae Int J Med Microbiol 2014, 304 (3-4), 490-498. S1438-42 DOI: 10.1016/j.ijmm.2014.02.010

[16] Seper, A., Hosseinzadeh, A., Gorkiewicz, G., Lichtenegger, S., Roier, S., Leitner, D.R., Rohm, M., Grutsch, A., Reidl, J., Urban, C.F., Schild, S. Vibrio cholerae Evades Neutrophil Extracellular Traps by the Activity of Two Extracellular Nucleases PLoS Pathog 2013, 9 (9), e1003614. DOI: 10.1371/journal.ppat.1003614

[17] Roier, S., Fenninger, J.C., Leitner, D.R., Rechberger, G.N., Reidl, J., Schild, S. Immunogenicity of Pasteurella multocida and Mannheimia haemolytica outer membrane vesicles Int J Med Microbiol 2013, 303 (5), 247-256. S1438-4221(13)00065-9 DOI: 10.1016/j.ijmm.2013.05.001

[18] Moisi, M., Lichtenegger, S., Tutz, S., Seper, A., Schild, S., Reidl, J. Characterizing the Hexose-6-Phosphate Transport System of Vibrio cholerae, a Utilization System for Carbon and Phosphate Sources J Bacteriol 2013, 195 (8), 1800-1808. DOI: 10.1128/JB.01952-12

[19] Leitner, D.R., Feichter, S., Schild-Prufert, K., Rechberger, G.N., Reidl, J., Schild, S. Lipopolysaccharide modifications of a cholera vaccine candidate based on outer membrane vesicles reduce endotoxicity and reveal the major protective antigen Infect Immun 2013, 81 (7), 2379-2393. DOI: 10.1128/IAI.01382-12

[20] Lees-Miller, R.G., Iwashkiw, J.A., Scott, N.E., Seper, A., Vinogradov, E., Schild, S., Feldman, M.F. A common pathway for O-linked protein-glycosylation and synthesis of capsule in Acinetobacter baumannii Mol Microbiol 2013, 89 (5), 816-830. DOI: 10.1111/mmi.12300


[1] Cakar F, Zingl FG, Moisi M, Reidl J, Schild S. In vivo repressed genes of Vibrio cholerae reveal inverse requirements of an H+/Cl- transporter along the gastrointestinal passage. Proc Natl Acad Sci U S A 2018, 115(10):E2376-E2385. DOI: 10.1073/pnas.1716973115

[2] Roier, S., Zingl, F.G., Cakar, F., Durakovic, S., Kohl, P., Eichmann, T.O., Klug, L., Gadermaier, B., Weinzerl, K., Prassl, R., Lass, A., Daum, G., Reidl, J., Feldman, M.F., Schild, S. A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria Nat Commun 2016, 7, 10515. DOI: 10.1038/ncomms10515

[3] Gumpenberger, T., Vorkapic, D., Zingl, F.G., Pressler, K., Lackner, S., Seper, A., Reidl, J., Schild, S. Nucleoside uptake in Vibrio cholerae and its role in the transition fitness from host to environment Mol Microbiol 2016, 99 (3), 470-483. DOI: 10.1111/mmi.13143

[4] Leitner, D.R., Lichtenegger, S., Temel, P., Zingl, F.G., Ratzberger, D., Roier, S., Schild-Prufert, K., Feichter, S., Reidl, J., Schild, S. A combined vaccine approach against Vibrio cholerae and ETEC based on outer membrane vesicles Front Microbiol 2015, 6, 823. DOI: 10.3389/fmicb.2015.00823

[5] Seper, A., Hosseinzadeh, A., Gorkiewicz, G., Lichtenegger, S., Roier, S., Leitner, D.R., Rohm, M., Grutsch, A., Reidl, J., Urban, C.F., Schild, S. Vibrio cholerae Evades Neutrophil Extracellular Traps by the Activity of Two Extracellular Nucleases PLoS Pathog 2013, 9 (9), e1003614. DOI: 10.1371/journal.ppat.1003614

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[6] Leitner, D.R., Feichter, S., Schild-Prufert, K., Rechberger, G.N., Reidl, J., Schild, S. Lipopolysaccharide modifications of a cholera vaccine candidate based on outer membrane vesicles reduce endotoxicity and reveal the major protective antigen Infect Immun 2013, 81 (7), 2379-2393. DOI: 10.1128/IAI.01382-12

[7] Roier, S., Leitner, D.R., Iwashkiw, J., Schild-Prufert, K., Feldman, M.F., Krohne, G., Reidl, J., Schild, S. Intranasal immunization with nontypeable Haemophilus influenzae outer membrane vesicles induces cross-protective immunity in mice PLoS One 2012, 7 (8), e42664. DOI: 10.1371/journal.pone.0042664

[8] Seper, A., Fengler, V.H., Roier, S., Wolinski, H., Kohlwein, S.D., Bishop, A.L., Camilli, A., Reidl, J., Schild, S. Extracellular nucleases and extracellular DNA play important roles in Vibrio cholerae biofilm formation Mol Microbiol 2011, 82 (4), 1015-1037. DOI: 10.1111/j.1365-2958.2011.07867.x

[9] Schild, S., Nelson, E.J., Bishop, A.L., Camilli, A. Characterization of Vibrio cholerae outer membrane vesicles as a candidate vaccine for cholera Infect Immun 2009, 77 (1), 472-484. DOI: 10.1128/IAI.01139-08

[10] Schild, S., Tamayo, R., Nelson, E.J., Qadri, F., Calderwood, S.B., Camilli, A. Genes induced late in infection increase fitness of Vibrio cholerae after release into the environment Cell Host Microbe 2007, 2 (4), 264-277. DOI: 10.1016/j.chom.2007.09.004

Patents

Roier S, Reidl J, Schild S. 2012. Vaccine against Pasteurellaceae. EP 2505208

Schild S, Nelson EJ, Camilli A. 2008. Cholera Vaccines. PCT/US08/879,292

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Curriculum Vitae – Univ.-Prof. Dr. rer. nat. Ulrich Stelzl Institute of Pharmaceutical Sciences (IPW), Department of Pharmaceutical Chemistry, University of Graz, Austria; Phone: +43 (0) 316 380 5397, E-Mail: [email protected] https://pharmazie.uni-graz.at/en/research/pharmaceutical-chemistry/network-pharmacology-stelzl/

Year of Birth *1971






Keywords network biology, protein-protein interactions, post-translational

modifications, computational biology, proteomics, genomics


since 09/2015 Full Professor at the Institute of Pharmaceutical Sciences, Department

of Pharmaceutical Chemistry, University of Graz and BioTechMed-Graz,

Austria

2007 - 2015 Max-Planck Research Group Leader (W2 position) at the Max-Planck

Institute for Molecular Genetics (MPIMG), Berlin, Germany

Head of the Otto Warburg Laboratory “Molecular Interaction Networks”

2002 - 2007 Postdoctoral fellow / NGFN project leader in the AG Neuroproteomics

headed by Erich Wanker, Max-Delbrück Center for Molecular Medicine

(MDC), Berlin-Buch, Germany

2001 - 2002 Research Fellow in the Laboratory of Dinshaw Patel at the Memorial

Sloan Kettering Cancer Center (MSKCC), New York, USA

2000 Postdoctoral fellow in the AG Ribosomen, MPIMG, Berlin

Education

1996 - 1999 Ph.D. thesis at the Max-Planck Institute Molecular Genetics, Berlin,

Germany in the AG Ribosomen under the supervision of Knud Nierhaus,

Academic degree Dr. rer. nat., University of Vienna

1994 - 1995 Studies of Biochemistry at the ETH Zurich, Switzerland

Diploma Thesis at the ETH Zurich, Laboratory for Organic Chemistry.

1990 - 1996 Studies of Technical Chemistry / Biochemistry at the TU Vienna

Graduated with honors, April 1996 at the TU Vienna


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34


2018 EMBL-EBI Seminar; 20th March 2018; EBI, Genome Campus, Hinxton, UK

2017 VDS lecture, Vienna Doctoral School Molecules of Life; Oct 9th, 2017;

IMBA Lecture Hall, Dr. Bohr-Gasse, Vienna, AT

2016 BioMed X Lunch Talk Series / DKFZ Postdoc Network; 22nd Sept 2016;

BioMed X Innovation Center / DKFZ, Heidelberg, Germany

2016 Workshop Network Biology; Apr. 11 – Apr. 15, 2016, Simons Institute for

the Theory of Computing, UC Berkeley, USA

2015 CSH Systems Biology: Networks, March 17 – 21, 2015, Cold Spring Harbor

Laboratory, New York, USA

Honors ▪ Awards

2008 Erwin Schrödinger Preis of the HGF (50 kEuro price money, shared)

http://www.helmholtz.de/ueber_uns/forschungspreise/erwin_schroedinger_preis/


since 2013 Special Section Organizer/Chair at ISMB 2013; Program committee

member of ISMB 2015, ISMB 2016, ISMB 2017; Organizing committee

Austrian Proteomic and Metabolomic Research Symposium (APRS 2017)

since 2013 Editorial Board of Scientific Reports

since 2010 Associate Editor BMC Bioinformatics

since 2007 Peer review for grant agencies / sci. societies incl. MPG (MPRGL and

Minerva Center, (DE)), BBSRC (UK), ANEP (ES), ANR (FR), MRC (UK);

GIF (IL/DE), BSF (IL) RGC (Hong Kong), NRF (Korea), USA Universitys,

Humbolt-Stiftung (DE), Leibnitz Gemeinschaft(DE), BioSysNet(DE)

since 2007 Peer review for more than 50 Journals incl. Nature, Nat Biotechnol, Nat

Methods, Science, Sci Signal, Mol Cell, Cell Systems, Genome Res,

Genome Biol, Nat Commun, Nat Protoc, Mol Syst Biol, elife, Nucleic Acids

Res, PLoS Genet, PLoS Comput Biol, Bioinformatics, BMC Bioinformatics,

BMC Biol, Trends Biochem Sci, Trends Biotechnol, Cell Res, Sci Rep-UK,

J Proteome Res, Proteomics, etc.

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Funded Projects


Funding Org.

Project Number

Research topic Amount funded [kEuro]

Funding Period

FWF P 30162 Amino acid resolution mapping of

interactions of NF2 402 2018-2020

Max Planck Society

Project grant The human RNA-binding protein

interactome 78 2015

Max Planck Society

Max-Planck Research

Group Molecular Interaction Networks 2900 2007–2016


Patrick ALOY, IRB Barcelona, Spain

Miguel ANDRADE-NAVARRO, IMB Mainz, Germany

Thomas SCHWARTZ, MIT, Boston, USA

Bernd TIMMERMANN, MPIMG, Berlin, Germany

Markus WAHL, FU Berlin, Germany


P. ALOY, IRB Barcelona, Spain: Structural interactome modelling

M. ANDRADE-NAVARRO, IMB Mainz, Germany: Bioinformatics

R. SHARAN, Tel Aviv University, Israel: Bioinformatics;

B. TIMMERMANN, MPIMG Berlin, Germany: Second generation sequencing

H. HERMJAKOB, EMBL-EBI, Hinxton, UK: Database bioinformatics (INTACT, UniProt)

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[1] Woodsmith J, Stelzl U; Understanding Disease Variants through the Lens of Protein Interactions; Cell Syst 2017, 5 (6): 544-546; DOI: 10.1016/j.cels.2017.12.009

[2] Woodsmith J, Apelt L, Casado-Medrano V, Özkan Z, Timmermann B, Stelzl U; Protein interaction perturbation profiling at amino acid resolution; Nat Methods 2017, 14(12): 1213-21; DOI: 10.1038/nmeth.4464

[3] Corwin T, Woodsmith J, Apelt F, Fontaine JF, Meierhofer D, Helmuth J, Grossmann A, Andrade-Navarro MA, Ballif BA, Stelzl U; Defining Human Tyrosine Kinase Phosphorylation Networks Using Yeast as an In Vivo Model Substrate; Cell Syst 2017, 5 (2): 128-139, DOI: 10.1016/j.cels.2017.08.001

[4] Birth P, Schöne S, Stelzl U, Meijsing SH; Identification and characterization of BATF3 as a context-specific coactivator of the glucocorticoid receptor; PLoS One 2017, 12 (7), e0181219; DOI: 10.1371/journal.pone.0181219

[5] Henning LM, Santos KF, Sticht J, Jehle S, Lee CT, Wittwer M, Urlaub H, Stelzl U, Wahl MC, Freund C; A new role for FBP21 as regulator of Brr2 helicase activity; Nucleic Acids Res 2017, 45(13): 7922–37; DOI: 10.1093/nar/gkx535

[6] Woodsmith J, Stelzl U, Vinayagam A; Bioinformatics Analysis of PTM-Modified Protein Interaction Networks and Complexes; Methods Mol Biol 2017, 1558: 321–332; DOI: 10.1007/978-1-4939-6783-4_15

[7] Böhme MA, Beis C, Reddy-Alla S, Reynolds E, Mampell MM, Grasskamp AT, Lützkendorf J, Bergeron DD, Driller JH, Babikir H, Göttfert F, Robinson IM, O'Kane CJ, Hell SW, Wahl MC, Stelzl U, Loll B, Walter AM, Sigrist SJ. Active zone scaffolds differentially accumulate Unc13 isoforms to tune Ca2+ channel-synaptic vesicle coupling Nat Neurosci 2016, 19: 1311-1320. DOI: 10.1038/nn.4364

[8] Bachmann VA, Mayrhofer JE, Ilouz R, Tschaikner P, Raffeiner P, Röck R, Courcelles M, Apelt F, Lu TW, Baillie GS, Thibault P, Aanstad P, Stelzl U, Taylor SS, Stefan E. Gpr161 anchoring of PKA consolidates GPCR and cAMP signaling Proc Natl Acad Sci U S A 2016, 113: 7786–7791. DOI: 10.1073/pnas.1608061113

[9] Apelt L, Knockenhauer KE, Leksa NC, Benlasfer N, Schwartz TU, Stelzl U. Systematic protein-protein interaction analysis reveals inter-subcomplex contacts in the nuclear pore complex Mol Cell Proteomics 2016, 15: 2594-2606. DOI: 10.1074/mcp.M115.054627

[10] Weir ME, Mann JE, Corwin T, Fulton ZW, Hao JM, Maniscalco JF, Kenney MC, Roman Roque KM, Chapdelaine EF, Stelzl U, Deming PB, Ballif BA, Hinkle KL. Novel Sites of Src Family Kinase Autophosphorylation Regulate Kinase Activity and SH2 Domain Binding Capacity FEBS Lett 2016, 590:1042–1052. DOI: 10.1002/1873-3468.12144

[11] Schütze T, Ulrich AK, Apelt L, Will CL, Bartlick N, Seeger M, Weber G, Lührmann R, Stelzl U#, Wahl MC#. Multiple protein-protein interactions converging on the Prp38 protein during activation of the human spliceosome RNA 2016, 22: 265-277. DOI: 10.1261/rna.054296.115

[12] Absmeier E, Rosenberger L, Apelt L, Becke C, Santos KF, Stelzl U, Wahl MC. A noncanonical PWI domain in the N-terminal helicase-associated region of the spliceosomal Brr2 protein Acta Crystallogr D Biol Crystallogr 2015, 71: 762-771. DOI: 10.1107/S1399004715001005

[13] Grossmann A, Benlasfer N, Birth P, Hegele A, Wachsmuth F, Apelt L, Stelzl U. Phospho-tyrosine dependent protein-protein interaction network Mol Syst Biol 2015, 11: 794. DOI: 10.15252/msb.20145968

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[14] Stelzl U. E. coli network upgrade Nat Biotechnol 2014, 32: 241-243. DOI:10.1038/nbt.2848

[15] Woodsmith J, Stelzl U. Studying post-translational modifications with protein interaction networks Curr Opin Struct Biol 2014, 24: 34-44. DOI: 10.1016/j.sbi.2013.11.009

[16] Kamburov A, Stelzl U, Lehrach H, Herwig R. The ConsensusPathDB interaction database: 2013 update Nucleic Acids Res 2013, 41: D793-800. DOI: 10.1093/nar/gks1055

[17] Stelzl U. Molecular interaction networks in the analyses of sequence variation and proteomics data Proteomics Clinical applications 2013, 7: 727-732. DOI: 10.1002/prca.201300039

[18] Weimann M, Grossmann A, Woodsmith J, Ozkan Z, Birth P, Meierhofer D, Benlasfer N, Valovka T, Timmermann B, Wanker EE, Sauer S, Stelzl U. A Y2H-seq approach defines the human protein methyltransferase interactome Nat Methods 2013,10: 339-342. DOI: 10.1038/nmeth.2397

[19] Woodsmith J, Kamburov A, Stelzl U. Dual coordination of post translational modifications in human protein networks. PLoS Comput Biol 2013, 9: e1002933. DOI: 10.1371/journal.pcbi.1002933


[1] Woodsmith J, Apelt L, Casado-Medrano V, Özkan Z, Timmermann B, Stelzl U; Protein interaction perturbation profiling at amino acid resolution; Nat Methods 2017, 14(12): 1213-21; DOI: 10.1038/nmeth.4464

[2] Corwin T, Woodsmith J, Apelt F, Fontaine JF, Meierhofer D, Helmuth J, Grossmann A, Andrade-Navarro MA, Ballif BA, Stelzl U; Defining Human Tyrosine Kinase Phosphorylation Networks Using Yeast as an In Vivo Model Substrate; Cell Syst 2017, 5(2): 128–39; DOI: 10.1016/j.cels.2017.08.001

[3] Grossmann A, Benlasfer N, Birth P, Hegele A, Wachsmuth F, Apelt L, Stelzl U. Phospho-tyrosine dependent protein-protein interaction network Mol Syst Biol 2015, 11, 794. DOI: 10.15252/msb.20145968

[4] Woodsmith J, Kamburov A, Stelzl U. Dual coordination of post translational modifications in human protein networks PLoS Comput Biol 2013, 9(3), e1002933. DOI: 10.1371/journal.pcbi.1002933

[5] Weimann M, Grossmann A, Woodsmith J, Ozkan Z, Birth P, Meierhofer D, Benlasfer N, Valovka T, Timmermann B, Wanker EE, Sauer S, Stelzl U. A Y2H-seq approach defines the human protein methyltransferase interactome Nat Methods 2013, 10, 339-342. DOI: 10.1038/nmeth.2397

[6] Hegele A, Kamburov A, Grossmann A, Sourlis C, Wowro S, Weimann M, Will CL, Pena V, Lührmann R, Stelzl U. Dynamic protein-protein interaction wiring of the human spliceosome Mol Cell 2012, 45, 567-580. DOI: 10.1016/j.molcel.2011.12.034

[7] Vinayagam A=, Stelzl U=#, Foulle R, Plassmann S, Zenkner M, Timm J, Assmus HE, Andrade-Navarro MA, Wanker EE#. A directed protein interaction network for investigating intracellular signal transduction Sci Signal 2011, 4, rs8. DOI: 10.1126/scisignal.2001699

[8] Venkatesan K,= Rual JF=, Vazquez A=, Stelzl U=, Lemmens I=, Hirozane-Kishikawa T, Hao T, Zenkner M, Xin X, Goh KI, Yildirim MA, Simonis N, Heinzmann K, Gebreab F, Sahalie JM, Cevik S, Simon C, de Smet AS, Dann E, Smolyar A, Vinayagam A, Yu H, Szeto D, Borick H, Dricot A, Klitgord N, Murray RR, Lin C, Lalowski M, Timm J, Rau K, Boone C, Braun P, Cusick ME, Roth FP, Hill DE, Tavernier J, Wanker EE, Barabási AL,

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Vidal M. An empirical framework for binary interactome mapping Nat Methods 2009, 6, 83-90. DOI: 10.1038/nmeth.1280

[9] Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE. A Human Protein-Protein Interaction Network: A Resource for Annotating the Proteome Cell 2005, 122, 957-968. DOI: 10.1016/j.cell.2005.08.029

[10] Stelzl U, Spahn CM, Nierhaus KH. Selecting rRNA binding sites for the ribosomal proteins L4 and L6 from randomly fragmented rRNA: Application of a method called SERF Proc Natl Acad Sci U S A 2000, 97, 4597 - 4602. DOI: 10.1073/pnas.090009297

= contributed equally, # corresponding authors

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39

Curriculum Vitae – Ao. Univ.-Prof. Dr. rer. nat. Klaus Zangger Institute of Chemistry, University of Graz, Heinrichstrasse 28, Ground floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 8673, E-Mail: [email protected] http://nmr.uni-graz.at

Year of Birth *1970




h-Index 28 (Scopus, March 2018)


Keywords NMR spectroscopy, structure and function of proteins, protein-

protein/DNA/lipid interaction, toxin-antitoxin systems, pure shift NMR,

biophysics, biochemistry


since 2005 Head of NMR Lab, Institute of Chemistry, University of Graz, Austria

since 2004 Associate professor, Institute of Chemistry, University of Graz, Austria

2003 Habilitation in Chemistry and appointment as “Dozent”

2001 - 2003 Assistant professor, Institute of Chemistry, University of Graz, Austria

2000 - 2001 APART Fellowship at the Institute of Chemistry at the University of Graz

1999 - 2000 Post-doc at the Institute of Pharmaceutical Chemistry at the University

of Graz, Austria

1997 - 1999 Post-doc at the Institute of Biochemistry, Molecular Biology and

Biophysics at the University of Minnesota at Minneapolis, USA (Prof. Ian

M. Armitage), (E. Schrödinger fellowship)

1993 - 1996 Assistant at the Institute of Organic Chemistry at the University of Graz

Education

1993 - 1996 PhD thesis (with distinction)

University of Graz, Austria, Supervisor: Prof. Dr. H. Sterk

1988 - 1996 Study in Chemistry (with distinction), University of Graz, Austria


2017 Euromar Conference, Warsaw, Poland

2017 Analytical Methods in Structure Biology Conference, Hamburg, Germany

2015 ENC Conference, Monterey, USA.


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40

2014 SMASH NMR Conference, Atlanta, Georgia, USA.

2014 Euromar Conference, Zurich, Switzerland.

Honors ▪ Awards

2000 - 2001 APART Fellowship (Austrian Academy of Sciences)

1997 - 1999 Erwin Schrödinger Fellowship (Austrian Science Funds FWF)


since 2016 Organizer of the biannual NMR Summerschool Niederöblarn

since 2010 Organization of several workshops/symposia, like the CEUM 2010,

Austrian BioNMR Meeting 2016

since 2006 Reviewer for several journals (e.g. JACS, PNAS, Angew.Chem, JBC)

since 2011 Reviewer for several granting agencies (e.g. DFG (Germany), ANR

(France), ETH Zurich, EU projects.

since 2012 Member in various habilitation and professor hiring commissions,

University of Graz, Austria



Funding Org.

Project Number

Research topic Amount funded [kEuro]

Funding Period

FWF P30230 Interrupted NMR pulse-sequences 308 2017-2020

FWF P27793 Spatially-selective NMR

spectroscopy 273 2015-2017

FWF P24742 Pure shift NMR 276 2012-2015



559 2011-2019

FWF P22630 NMR studies on the relaxosome 272 2010-2013

Top 5 International Collaborations (since 2013)

Wolfgang BERMEL, Bruker BioSpin, Rheinstetten, Germany

Mark GLOVER, Department of Biochemistry, University of Alberta, Edmonton, Canada

Remy LORIS, Center for Structural Biology, Free University of Brussels, Belgium

Peyman SAKHAII, Sanofi, Aventis, Frankfurt, Germany

Predrag NOVAK, Faculty of Natural Sciences, University of Zagreb, Croatia

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I. FELLI, CERM, University of Florence, Italy: High field NMR, paramagnetics and direct

carbon detection

R. WIMMER, Aalborg University, Denmark: Protein-protein and protein ligand interactions

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[1] Becker, W., Adams, L.A., Graham, B., Wagner, G.E., Zangger, K., Otting, G., Nitsche, C.,Trimethylsilyl tag for probing protein-ligand interactions by NMR. J. Biomol. NMR 2018, doi: 10.1007/s10858-018-0173-6

[2] Becker, W., Bhattiprolu, K.C., Gubensäk, N., Zangger, K., Investigating Protein-Ligand Interactions by Solution Nuclear Magnetic Resonance Spectroscopy. ChemPhysChem 2018, doi: 10.1002/cphc.201701253

[3] Buchberger, K., Walenta, M., Zangger, K., Extracting unresolved coupling constants from complex multiplets by a real-time J-upscaled SERF experiment. Magn Reson Chem 2018, doi: 10.1002/mrc.4699

[4] Dal Cortivo, G., Wagner, G.E., Cortelletti, P., Padmanabha Das, K.M., Zangger, K., Speghini, A., Dell'Orco, D., Meyer, N.H., Luminescent and paramagnetic properties of nanoparticles shed light on their interactions with proteins. Sci Rep 2018, 8(1), 3420. doi: 10.1038/s41598-018-21571-y

[5] Dornisch, E., Pletz, J., Glabonjat, R.A., Martin, F., Lembacher-Fadum, C., Neger, M., Högenauer, C., Francesconi, K., Kroutil, W., Zangger, K., Breinbauer, R., Zechner, E.L., Biosynthesis of the Enterotoxic Pyrrolobenzodiazepine Natural Product Tilivalline. Angew Chem Int Ed 2017, 56(46), 14753-14757, doi: 10.1002/anie.201707737

[6] Glabonjat, R.A., Raber, G., Jensen, K.B., Guttenberger, N., Zangger, K., Francesconi, K.A., A 2-O-Methylriboside Unknown Outside the RNA World Contains Arsenic. Angew Chem Int Ed 2017, 56(39), 11963-11965, doi: 10.1002/anie.201706310

[7] Göbl, C., Focke-Tejkl, M., Najafi, N., Schrank, E., Madl, T., Kosol, S., Madritsch, C., Dorofeeva, Y., Flicker, S., Thalhamer, J., Valenta, R., Zangger, K., Tjandra, N., Flexible IgE epitope-containing domains of Phl p 5 cause high allergenic activity. J Allerg Clin Immunol 2017, 140(4), 1187-1191, DOI: 10.1016/j.jaci.2017.05.005

[8] Lienhart, W., Strandback, E., Gudipati, V., Koch, K., Binter, A., Uhl, M.K. et al. Catalytic competence, structure and stability of the cancer associated R139W variant of the human NAD(P)H:quinone oxidoreductase 1 (NQO1) FEBS J 2017, 284(8), 1233-124 DOI: 10.1111/febs.14051

[9] Fercher, C., Keller, W., Zangger, K., Meyer, N.H. 1H, 15N and 13C chemical shift assignment of the Gram-positive conjugative transfer protein TraHpIP501 Biomol NMR Assign 2016, 10 (1),163–166. DOI: 10.1007/s12104-015-9658-3

[10] Fercher, C., Probst, I., Kohler, V., Goessweiner-Mohr, N., Arends, K., Grohmann, E., Zangger, K., Meyer, N.H., Keller, W. et al. VirB8-like protein TraH is crucial for DNA transfer in Enterococcus faecalis Scientific Rep 2016, 6, 24643. DOI: 10.1038/srep24643

[11] Reichart, B., Guedes de la Cruz, G., Zangger, K., Kappe, C.O., Glasnov, T. Copper/Nafion-Catalyzed Hydroarylation Process Involving Ketenimine Intermediates. A Novel and Synthetic Approach to 4-Sulfonamidoquinoline-2-ones and Derivatives Thereof Adv Synth Catal 2016, 358 (1), 50–55. DOI: 10.1002/adsc.201500942

[12] Guttenberger, N., Glabonjat, R.A., Jensen, K.B., Zangger, K., Francesconi, K.A. Synthesis of two arsenic-containing cyclic ethers. Model compounds for a novel group of naturally-occurring arsenolipids Tetrahedron Lett 2016, 57 (41), 4578–4580. DOI: 10.1016/j.tetlet.2016.08.097

[13] Glanzer, S., Kunert, O., Zangger, K. Determination of unresolved heteronuclear scalar coupling constants by J(up)-HSQMBC J Magn Reson 2016, 268,88–94. DOI: 10.1016/j.jmr.2016.05.002

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[14] Nusshold, C., Ullen, A., Kogelnik, N., Bernhart, E., Reicher, H., Plastira, I., Glasnov, T., Zangger, K., Rechberger, G., Kollroser, M., Fauler, G., Wolinski, H., Weksler, B.B., Romero, I.A., Kohlwein, S.D., Couraud, P.O., Malle, E., Sattler, W. Assessment of electrophile damage in a human brain endothelial cell line utilizing a clickable alkyne analog of 2-chlorohexadecanal Free Radic Biol Med 2016, 90,59–74. DOI: 10.1016/j.freeradbiomed.2015.11.010

[15] Roier, S., Blume, T., Klug, L., Wagner, G.E., Elhenawy, W., Zangger, K., Prassl, R., Reidl, J., Daum, G., Feldman, M.F., Schild, S. A basis for vaccine development: Comparative characterization of Haemophilus influenzae outer membrane vesicles Int J Med Microbiol 2015, 305 (3), 298-309. DOI: 10.1016/j.ijmm.2014.12.005

[16] Zangger, K. (2015): Pure shift NMR Progr NMR Spectr 2015, 86-87C, 1–20. DOI: 10.1016/j.pnmrs.2015.02.002

[17] Glanzer, S., Pulido, S.A., Tutz, S., Wagner, G.E., Kriechbaum, M., Gubensäk, N., Trifunovic, J., Dorn, M., Fabia, W.M.F., Novak, P., Reidl, J., Zangger, K. Structural and functional implications of the interaction between macrolide antibiotics and bile acids Chemistry-Eur J 2015, 21 (11), 4350–4358. DOI: 10.1002/chem.201406413

[18] Zorzini, V., Buts, L., Schrank, E., Sterckx, Y.G.J., Respondek, M., Engelberg-Kulka, H. et al. Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding Nucl Acids Res 2015, 43 (2), 1241–1256. DOI: 10.1093/nar/gku1352

[19] Dupe, A., Judmaier, M.E., Belaj, F., Zangger, K., Mösch-Zanetti, N.C. Activation of molecular oxygen by a molybdenum complex for catalytic oxidation Dalton Trans 2015, 44 (47), 20514–20522. DOI: 10.1039/c5dt02931g

[20] Glanzer, S., Zangger, K. Uniform Reduction of Scalar Coupling by Real-Time Homonuclear J-Downscaled NMR Chemphyschem 2015, 16 (15), 3313–3317. DOI: 10.1002/cphc.201500640

[21] Glanzer, S., Zangger, K. Visualizing unresolved scalar couplings by real-time J-upscaled NMR J Am Chem Soc 2015, 137 (15), 5163–5169. DOI: 10.1021/jacs.5b01687.

[22] Mauhart, J., Glanzer, S., Sakhaii, P., Bermel, W., Zangger, K. Faster and cleaner real-time pure shift NMR experiments J Magn Reson 2015, 259, 207–215. DOI: 10.1016/j.jmr.2015.08.011

[23] Amanpour, T., Zangger, K., Belaj, F., Bazgir, A., Dallinger, D., Kappe, C.O. A detailed investigation of the multicomponent reaction of salicylaldehyde, ethyl acetoacetate and isocyanides under microwave heating Tetrahedron 2015, 71 (39), 7159–7169. DOI: 10.1016/j.tet.2014.12.002

[24] Boeszoermenyi, A., Nagy, H.M., Arthanari, H.,,Pillip, C.J., Lindermuth, H., Luna, R.E., Wagner, G., Zechner, R., Zangger, K., Oberer, M. Structure of a CGI-58 motif provides the molecular basis of lipid droplet anchoring J Biol Chem. 2015, 290 (44), 26361–26372. DOI: 10.1074/jbc.M115.682203

[25] Ullen, A., Nusshold, C., Glasnov, T., Saf, R., Cantillo, D., Eibinger, G. et al. Covalent adduct formation between the plasmalogen-derived modification product 2-chlorohexadecanal and phloretin Biochem Pharmacol 2015, 93 (4), 470–481. DOI: 10.1016/j.bcp.2014.12.017

[26] Vogel, A., Schmiedel, R., Hofmann, U., Gruber, K., Zangger, K. Converting Aspartase into a β-Amino Acid Lyase by Cluster Screening ChemCatChem 2014, 6 (4), 965–968. DOI: 10.1002/cctc.201300986

[27] Glanzer, S., Zangger, K. Directly decoupled diffusion-ordered NMR spectroscopy for the analysis of compound mixtures Chemistry-Eur J 2014, 20 (35), 11171–11175. DOI: 10.1002/chem.201402920

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[28] Gubensäk, N., Fabian, W.M.F., Zangger, K. Disentangling scalar coupling patterns by real-time SERF NMR Chem Commun 2014, 50 (82), 12254–12257. DOI: 10.1039/c4cc05892e

[29] Dall'antonia, F., Pavkov-Keller, T., Zangger, K., Keller, W. Structure of allergens and structure based epitope predictions Methods 2014, 66 (1), 3–21. DOI: 10.1016/j.ymeth.2013.07.024

[30] Hlina, J., Arp, H., Walewska, M., Florke, U., Zangger, K., Marschner, C., Baumgartner, J. Coordination Chemistry of Cyclic Disilylated Germylenes and Stannylenes with Group 11 Metals Organometallics 2014, 33 (24), 7069–7077. DOI: 10.1021/om500668r

[31] Lienhart, W., Gudipati, V., Uhl, M.K., Binter, A., Pulido, S.A., Saf, R. et al. Collapse of the native structure caused by a single amino acid exchange in human NAD(P)H:quinone oxidoreductase FEBS J 2014, 281 (20), 4691–4704. DOI: 10.1111/febs.12975

[32] Meyer, N.H., Zangger, K. Viva la Resolucion. Enhancing the Resolution of H-1 NMR Spectra by Broadband Homonuclear Decoupling Synlett 2014, 25 (7), 920–927. DOI: 10.1055/s-0033-1340635

[33] Meyer, N.H., Zangger, K. Boosting the resolution of 1H NMR spectra by homonuclear broadband decoupling Chemphyschem 2014, 15 (1), 49–55. DOI: 10.1002/cphc.201300861

[34] Meyer, N.H., Zangger, K. Enhancing the resolution of multi-dimensional heteronuclear NMR spectra of intrinsically disordered proteins by homonuclear broadband decoupling. Chem Commun 2014, 50 (12), 1488–1490. DOI: 10.1039/c3cc48135b

[35] Wagner, G.E., Gutfreund, S., Fauland, K., Keller, W., Valenta, R., Zangger, K. (2014): Backbone resonance assignment of Alt a 1, a unique beta-barrel protein and the major allergen of Alternaria alternata Biomol NMR Assign 2014, 8 (2), 229–231. DOI: 10.1007/s12104-013-9489-z

[36] Pratter, S.M., Ivkovic, J., Birner-Gruenberger, R., Breinbauer, R., Zangger, K., Straganz, G.D. More than just a halogenase: modification of fatty acyl moieties by a trifunctional metal enzyme Chembiochem 2014, 15 (4), 567–574. DOI: 10.1002/cbic.201300345

[37] Praznik, W., Löppert, R., Cruz Rubio, J.M., Zangger, K., Huber, A. Structure of fructo-oligosaccharides from leaves and stem of Agave tequilana Weber, var. azul Carbohyd Res 2013, 381, 64–73. DOI: 10.1016/j.carres.2013.08.025

[38] Redzej, A., Ilangovan, A., Lang, S., Gruber, C.J., Topf, M., Zangger, K. et al. Structure of a translocation signal domain mediating conjugative transfer by type IV secretion systems Mol. Microbiol 2013, 89 (2), 324–333. DOI: 10.1111/mmi.12275

[39] Wagner, G.E., Sakhaii, P., Bermel, W., Zangger, K. Monitoring fast reactions by spatially-selective and frequency-shifted continuous NMR spectroscopy. application to rapid-injection protein unfolding Chem Commun 2013, 49 (30), 3155–3157. DOI: 10.1039/c3cc39107h

[40] Wuensch, C., Lechner, H., Glueck, S.M., Zangger, K., Hall, M., Faber, K. Asymmetric Biocatalytic Cannizzaro-Type Reaction ChemCatChem 2013, 5 (7), 1744–1748. DOI: 10.1002/cctc.201300028

[41] Sakhaii, P., Haase, B., Bermel, W., Kerssebaum, R., Wagner, G. E., Zangger, K. Broadband homodecoupled NMR spectroscopy with enhanced sensitivity J Magn Reson 2013, 233, 92–95. DOI: 10.1016/j.jmr.2013.05.008

[42] Schrank, E., Wagner, G.E., Zangger, K. Solution NMR studies on the orientation of membrane-bound peptides and proteins by paramagnetic probes Molecules 2013, 18 (7), 7407–7435. DOI: 10.3390/molecules18077407

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[43] Hocking, Henry G., Zangger, Klaus, Madl, Tobias (2013): Studying the structure and dynamics of biomolecules by using soluble paramagnetic probes Chemphyschem 2013, 14 (13), 3082–3094. DOI: 10.1002/cphc.201300219

[44] Meyer, N.H., Zangger, K. Simplifying proton NMR spectra by instant homonuclear broadband decoupling Angew Chem Int Ed 2013, 52 (28), 7143–7146. DOI: 10.1002/anie.201300129

[45] Glanzer, S., Schrank, E., Zangger, K. A general method for diagonal peak suppression in homonuclear correlated NMR spectra by spatially and frequency selective pulses. J Magn Reson 2013, 323, 1–6. DOI: 10.1016/j.jmr.2013.04.005


[1] Zangger, K. (2015): Pure shift NMR Progr NMR Spectr 2015, 86-87C, 1–20. DOI: 10.1016/j.pnmrs.2015.02.002

[2] Glanzer, S., Zangger, K. Visualizing unresolved scalar couplings by real-time J-upscaled NMR J Am Chem Soc 2015, 137 (15), 5163–5169. DOI: 10.1021/jacs.5b01687

[3] Wagner, G.E., Sakhaii, P., Bermel, W., Zangger, K. Monitoring fast reactions by spatially-selective and frequency-shifted continuous NMR spectroscopy. application to rapid-injection protein unfolding Chem Commun 2013, 49 (30), 3155–3157. DOI: 10.1039/c3cc39107h

[4] Meyer, N.H., Zangger, K. Simplifying proton NMR spectra by instant homonuclear broadband decoupling Angew Chem Int Ed 2013, 52 (28), 7143–7146. DOI: 10.1002/anie.201300129

[5] Falsone,F., Meyer, N.H., Schrank, E., Leitinger, G., Pham, C.L., Fodero-Tavoletti, M.T., Holmberg, M., Dulle, M., Scicluna, B., Gesselbauer, B., Rückert, H.M., Wagner, G.E., Merle, D.A., Nollen, E.A., Kungl, A.J., Hill, A.F., Cappai, R., Zangger,K. SERF protein is a direct modifier of amyloid fiber assembly Cell Rep 2012, 2 (2), 358–371. DOI: 10.1016/j.celrep.2012.06.012

[6] Madl, T., Bermel, W., Zangger, K. Use of relaxation enhancements in a paramagnetic environment for the structure determination of proteins using NMR spectroscopy Angew Chem Int Ed 2009, 48 (44), S. 8259–8262. DOI: 10.1002/anie.200902561

[7] Jonge, N. de, Garcia-Pino, A., Buts, L., Haesaerts, S., Charlier, D., Zangger, K. et al. Rejuvenation of CcdB-poisoned gyrase by an intrinsically disordered protein domain. Mol Cell 2009, 35 (2), S. 154–163. DOI: 10.1016/j.molcel.2009.05.025

[8] Respondek, M., Madl, T., Göbl, C., Golser, R., Zangger, K. Mapping the orientation of helices in micelle-bound peptides by paramagnetic relaxation waves J Am Chem Soc 2007, 129 (16), S. 5228–5234. DOI: 10.1021/ja069004f

[9] Madl, T., van Melderen, L., Mine, N., Respondek, M., Oberer, M., Keller, W. et al. Structural basis for nucleic acid and toxin recognition of the bacterial antitoxin CcdA J Mol Biol 2006, 364 (2), 170–185. DOI: 10.1016/j.jmb.2006.08.082

[10] Zangger, K., Sterk, H. Homonuclear broadband-decoupled NMR spectra J Magn Reson 1997, 124, 486–489. DOI: 10.1006/jmre.1996.1063

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Curriculum Vitae – Ao. Univ.-Prof. Dr. rer. nat. Ellen L. Zechner Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50,1st floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 5624, E-Mail: [email protected] https://molekularbiologie.uni-graz.at/en/research/areas-of-research/infektionsbiologie/

Year of Birth *1961

Nationality USA / Austrian





Keywords host-pathogen interaction, bacterial enterotoxin, bacterial

macromolecular secretion, human microbiota, horizontal gene transfer


since 2016 Vice-President, Biology and Medicine Section, Austrian Science Fund

(FWF)

2015 - 2016 Vice Dean of the Natural Sciences Faculty, University of Graz

NAWI-Graz Steering Committee, University of Graz / Technical Univ. of

Graz cooperative research and teaching program

Supervisory Board, Doctoral Academy Graz, University of Graz

2013 - 2017 Advisory Board, Doktoratskolleg Molecular Fundamentals of

Inflammation (MOLIN), Medical University of Graz

2011 - 2016 Austrian Science Fund (FWF), Full Member of the Kuratorium

Fields of Genetics, Microbiology and Biotechnology

2009 - 2016 Spokesperson for the University of Graz core research area “Molecular

Enzymology and Physiology”

2007 - 2016 Director, Doctoral School of Molecular Biology and Biochemistry,

University of Graz

2006 - 2015 Member, COIMBRA Universities’ Task Force “Doctoral Studies and

Research”

2006 - 2008 Treasurer, International Society for Plasmid Biology and other Mobile

Genetic Elements

since 2005 Director of the Doktoratskolleg Molecular Enzymology, University of

Graz and Graz University of Technology, Austria


E8: E.L. Zechner

47

since 2001 Ao.University Professor, Institute of Molecular Biosciences, University of

Graz, Habilitation: Molecular Microbiology

2001 Habilitation (‚venia docendi‘), University of Graz

1999 - 2001 University Assistant, Institute of Molecular Biosciences, University of

Graz

1994 - 1999 University Assistant, Institute of Microbiology, University of Graz

1993 Maternity Leave

1992 - 1994 Postdoctoral fellow, Institute of Microbiology, University of Graz

Education

1986 - 1991 Ph.D. in Molecular Biology, Sloan-Kettering Division, Cornell University,

Graduate School of Medical Sciences, New York, USA Supervisor: Prof.

Dr. K. J. Marians

1979 - 1983 B.A. degree in Chemistry and Biology, Oberlin College, Oberlin, USA


2016 “Functional interaction network of bacterial FIC effector proteins”, Type

IV secretion in Gram-negative and Gram-positive bacteria, Beilngries,

DE

2014 “Bacteriophage as structure-function probes of T4SS” Biophysical

Aspects of Type IV Secretion, Bilbao, ES

2013 “Late stage morphogenetic or gating reactions of the R1 conjugative

machinery require the actin-like partitioning protein ParM“, Santander,

ES

2011 “Coupling protein and substrate selection in the F-like T4SS R1“, Baeza,

ES

2011 “Regulation of plasmid conjugation“, Institute Pasteur, FR

Honors ▪ Awards

2014 - 2015 Prizes awarded for Schneditz, G. et al. (2014) Enterotoxicity of a

nonribosomal peptide causes antibiotic-associated colitis. Proc Natl

Acad Sci U S A 111: 13181-13186

(€10,000) Top Abstract Award 2014, United European

Gastroenterologists, Berlin

(€4,000) Wewalka Prize 2015, Salzburg

E8: E.L. Zechner

48

Career Related Activities in Editorial Boards, as Reviewers and Memberships

2002 - 2011 Member of Elsevier Journal Editorial Board, Plasmid

2003 - 2006 Member of Elsevier Journal Editorial Board, Research in Microbiology

since 2005 Speaker of the Doktoratskolleg Molecular Enzymology, Graz, Austria

E8: E.L. Zechner

49



Funding Org.

Project Number


Funding Period

BioTechMed -

Immunomodulatory and Antiproliferative Bioactivities of the Bacterial Secretome

in Gastrointestinal Disease and Therapy (PI)

135 2017-2019

BioTechMed PostDoc Projects

Bacterial Metabolite signal molecules in the intestinal

microbiota 155 2015-2016

EFRE / Land Steiermark

A3-11.B-38/2012-7

Bacterial type IV protein translocation and Catheter-

associated urinary tract infections 310 2011-2014



559 2011-2019

FWF P24016 Regulation of the initiation of bacterial type IV secretion

326 2011-2016


Gabriel WAKSMAN, Structural and Molecular Biology, Birkbeck College, University College

London, UK

Martin J BLASER, New York University School of Medicine and VA Medical Center, New

York, NY, USA

Fernando DE LA CRUZ, Universidad de Cantabria, Santander, ES

Jose´ Fernando DÍAZ, Centro de Investigaciones Biológicas, CSIC, Madrid, ES

Joel F. SCHILDBACH, The Johns Hopkins University, Baltimore, MD, USA


C. DEHIO, University of Basel, CH; Biological diversity and structure-function relationships of

Fic domain proteins

K. GERDES, University of Copenhagen, DK; Function and regulation of TA modules

E8: E.L. Zechner

50


[1] Dornisch, E., J. Pletz, R. A. Glabonjat, F. Martin, C. Lembacher-Fadum, M. Neger, C. Hogenauer, K. Francesconi, W. Kroutil, K. Zangger, R. Breinbauer & E. L. Zechner, Biosynthesis of the Enterotoxic Pyrrolobenzodiazepine Natural Product Tilivalline. Angew Chem Int Ed Engl (2017) 56: 14753-14757. DOI: 10.1002/anie.201707737

[1] Ilangovan, A., Kay, C.W.M., Roier, S., El Mkami, H., Salvadori, E., Zechner, E.L., Zanettti, G., Waksman, G. Cryo-EM structure of a relaxase reveals the molecular basis of DNA unwinding during bacterial conjugation Cell 2017 169: 708-721 e712. DOI: 10.1016/j.cell.2017.04.010

[2] Kienesberger, S., Zechner, E.L., Helicobacter pylori Reference Module in Biomedical Sciences, BMED, 2017, Medical Microbiology BMED:99201

[3] Sprenger H.,S. Kienesberger, B. Pertschy, L. Poltl, B. Konrad, P. Bhutada, D. Vorkapic, D. Atzmuller, F. Feist, C. Hogenauer, G. Gorkiewicz & E. L. Zechner, (2017) Fic Proteins of Campylobacter fetus subsp. venerealis Form a Network of Functional Toxin-Antitoxin Systems. Front Microbiol 8: 1965. DOI: 10.3389/fmicb.2017.01965

[4] Zechner, E.L. Inflammatory disease caused by intestinal pathobionts Curr Opin Microbiol 2017, 35, 64-69. DOI: 10.1016/j.mib.2017.01.011

[5] Zechner, E.L., G. Moncalian & F. de la Cruz Relaxases and Plasmid Transfer in Gram-Negative Bacteria Curr Top Microbiol Immunol 2017, 413: 93-113.

[6] Gruber, C.J., Lang, S., Rajendra, V.K.H., Nuk, M., Raffl, S., Schildbach J.F., Zechner E.L. Conjugative DNA transfer is enhanced by plasmid R1 partitioning proteins Front Mol Biosci 2016, 3: 32. DOI: 10.3389/fmolb.2016.00032

[7] Kienesberger, S., Cox, L. M., Livanos, A., Zhang, X. S., Chung, J., Perez-Perez, G. I., Gorkiewicz, G., Zechner, E.L., Blaser, M.J. Gastric Helicobacter pylori Infection Affects Local and Distant Microbial Populations and Host Responses Cell Rep 2016, 14, 1395-1407. DOI: 10.1016/j.celrep.2016.01.017

[8] Zollner-Schwetz, I., Herzog, K. A., Feierl, G., Leitner, E., Schneditz, G., Sprenger, H., Prattes, J., Petritsch, W., Wenzl, H., Kump, P., Gorkiewicz, G., Zechner, E.L., Hogenauer C Dig Dis Sci 2015, 60 3393-8. DOI: 10.1007/s10620-015-3765-y

[9] Schneditz, G., Rentner, J., Roier, S., Pletz, J., Herzog, K. A., Bucker, R., Troeger, H., Schild, S., Weber, H., Breinbauer, R., Gorkiewicz, G., Högenauer, C., Zechner, E.L. Enterotoxicity of a nonribosomal peptide causes antibiotic-associated colitis Proc Natl Acad Sci U S A 2014, 111, 13181-13186. DOI: 10.1073/pnas.1403274111

[10] Kienesberger, S., Sprenger, H., Wolfgruber, S., Halwachs, B., Thallinger, G., Perez-Perez, G.I., Blaser, M.J., Zechner, E.L., Gorkiewicz G. Comparative genome analysis of Campylobacter fetus subspecies revealed horizontally acquired genetic elements important for virulence and niche specificity PLoS One 2014 9:e85491 DOI: 10.1371/journal.pone.0085491

[11] Gibert, M., Juarez, A., Zechner, E. L., Madrid, C., Balsalobre, C. TrhR, TrhY and HtdA, a novel regulatory circuit that modulates conjugation of the IncHI plasmids Mol Microbiol 2014, 94, 1146-1161. DOI: 10.1111/mmi.12823

[12] Reisner, A., Maierl, M., Jorger, M., Krause, R., Berger, D., Haid, A., Tesic D., Zechner, E.L. Type 1 fimbriae contribute to catheter-associated urinary tract infections caused by Escherichia coli J Bacteriol 2014, 196 931-939. DOI: 10.1128/JB.00985-13

[13] Herzog, K. A., Schneditz, G., Leitner, E., Feierl, G., Hoffmann, K.M., Zollner-Schwetz, I., Krause, R., Gorkiewicz, G., Zechner, E.L., Hogenauer, C. Genotypes of Klebsiella oxytoca Isolates from Patients with Nosocomial Pneumonia Are Distinct from Those of Isolates

E8: E.L. Zechner

51

from Patients with Antibiotic-Associated Hemorrhagic Colitis J Clin Microbiol 2014, 52, 1607-16. DOI: 10.1128/JCM.03373-13

[14] Lang, S., Gruber, C.J., Raffl, S., Reisner, A., Zechner, E.L. Common requirement for the relaxosome of plasmid R1 in multiple activities of the conjugative type IV secretion system. J Bacteriol 2014, 194, 2108-2121. DOI: 10.1128/JB.00045-13

[15] Redzej, A., Ilangovan, A., Lang, S., Gruber, C.J., Topf, M., Zangger, K., Zechner, E.L., Waksman G. Structure of a translocation signal domain mediating conjugative transfer by type IV secretion systems Mol Microbiol 2013, 89, 324-333. DOI: 10.1111/mmi.12275

[16] Alperi, A., Larrea, D., Fernandez-Gonzalez, E., Dehio, C., Zechner, E.L. Llosa, M. A translocation motif in relaxase TrwC specifically affects recruitment by its conjugative Type IV Secretion System J Bacteriol 2013, 195, 4999-5006. DOI: 10.1128/JB.00367-13


[2] Ilangovan, A., Kay, C.W.M., Roier, S., El Mkami, H., Zechner, E.L., Zanettti, G., Waksman, G. Cryo-EM structure of a relaxase reveals the molecular basis of DNA unwinding during bacterial conjugation Cell 2017, 169: 708-721 e712. DOI: 10.1016/j.cell.2017.04.010

[3] Dornisch, E., J. Pletz, R. A. Glabonjat, F. Martin, C. Lembacher-Fadum, M. Neger, C. Hogenauer, K. Francesconi, W. Kroutil, K. Zangger, R. Breinbauer & E. L. Zechner. Biosynthesis of the Enterotoxic Pyrrolobenzodiazepine Natural Product Tilivalline. Angew Chem Int Ed Engl 2017, 56: 14753-14757 DOI: 10.1002/anie.201707737

[4] Zechner, E.L. Inflammatory disease caused by intestinal pathobionts Curr Opin Microbiol 2017, 35, 64-69. DOI: 10.1016/j.mib.2017.01.011

[5] Kienesberger, S., Cox, L. M., Livanos, A., Zhang, X. S., Chung, J., Perez-Perez, G. I., Gorkiewicz, G., Zechner, E.L., Blaser, M.J. Gastric Helicobacter pylori Infection Affects Local and Distant Microbial Populations and Host Responses Cell Rep 2016, 14, 1395-1407. DOI: 10.1016/j.celrep.2016.01.017

[6] Schneditz, G., Rentner, J., Roier, S., Pletz, J., Herzog, K. A., Bucker, R., Troeger, H., Schild, S., Weber, H., Breinbauer, R., Gorkiewicz, G., Högenauer, C., , Zechner, E.L. Enterotoxicity of a nonribosomal peptide causes antibiotic-associated colitis Proc Natl Acad Sci U S A 2014, 111, 13181-13186. DOI: 10.1073/pnas.1403274111

[7] Sprenger H.,S. Kienesberger, B. Pertschy, L. Poltl, B. Konrad, P. Bhutada, D. Vorkapic, D. Atzmuller, F. Feist, C. Hogenauer, G. Gorkiewicz & E. L. Zechner. Fic Proteins of Campylobacter fetus subsp. venerealis Form a Network of Functional Toxin-Antitoxin Systems. Front Microbiol 2017, 8: 1965. DOI: 10.3389/fmicb.2017.01965

[8] Redzej, A., Ilangovan, A., Lang, S., Gruber, C.J., Topf, M., Zangger, K., Zechner, E.L., , Waksman G. Structure of a translocation signal domain mediating conjugative transfer by type IV secretion systems Mol Microbiol 2013, 89, 324-333. DOI: 10.1111/mmi.12275

[9] Lang, S.; Kirchberger, P.C.; Gruber, C.J.; Redzej, A.; Raffl, S.; Zellnig, G.; Zangger, K.; Zechner, E.L. An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation Mol Microbiol 2011, 82, 1071-1085. DOI:10.1111/j.1365-2958.2011.07872.x

[10] de la Cruz, F., Frost, L.S., Meyer, R.J., Zechner, E.L. Conjugative DNA metabolism in Gram-negative bacteria FEMS Microbiol Rev 2010, 34 18-40. DOI: 10.1111/j.1574-6976.2009.00195.x

[11] Reisner, A., Haagensen, J. A., Schembri, M. A., Zechner E. L., Molin, S. Development and maturation of Escherichia coli K-12 biofilms Mol Microbiol 2003, 48 933-946


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Curriculum Vitae – Univ.- Prof. Dr. Rudolf Zechner Institute of Molecular Biosciences, University of Graz, Heinrichstrasse 31, 2nd floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 1900, E-Mail: [email protected] https://molekularbiologie.uni-graz.at/en/research/areas-of-research/energy-and-lipid-metabolism/

Year of Birth *1954






Keywords biochemistry, molecular biology, cell biology of lipid and energy

metabolism


since 2016 Director BioTechMed-Graz, Austria

since 2000 Professor of Biochemistry, Institute of Molecular Biosciences, University

of Graz, Austria

1998 - 1999 Professor of Biochemistry, Chairman of the Institute of Biochemistry,

University of Graz, Austria

1994 - 1998 Head of the Section of Molecular Biology, Institute of Medical

Biochemistry, University of Graz, Austria

1990 - 1998 Associate Professor, Institute of Medical Biochemistry, University of

Graz, Austria

1990 Habilitation in Biochemistry, Institute of Medical Biochemistry, University

of Graz, Austria

1987 - 1990 University Assistant, Institute of Medical Biochemistry, University of

Graz, Austria

1985 - 1987 Research Associate, Laboratory of Biochemical Genetics and

Metabolism, The Rockefeller University, New York, USA

1980 - 1984 University Assistant, Institute of Medical Biochemistry, University of

Graz, Austria

Education

1980 Ph.D. Graduation in Chemistry, University of Graz, Austria

1972 - 1980 University education in General Chemistry, University of Graz, Austria


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53


2017 Keystone Symposium on Obesity and Diabetes (plenary lecture)

2015 Louis-Jeantet Prize Lecture at the EMBO Meeting Birmingham, UK

2014 Richard Havel Lecture, Deuel Lipid Conference, San Diego, CA, USA

2012 Award Lecture: DeWitt S. Goodman Lectureship, Columbia University,

NY, USA, sponsored by Columbia University, New York, USA

2012 Award Lecture: Van Deenen Lecture at the International Conference on

the Bioscience of Lipids and the American Society of Biochemistry and

Molecular Biology, Banff, Canada

Top 5 Honors and Awards

2018 Rolf Luft Award

2015 Louis-Jeantet Prize for Medicine

2013 Science Award, sponsored by the Country of Styria

2013 ERC Advanced Grant by the European Research Council

2009 European Lipid Science Award, sponsored by the European Federation

for the Science and Technology of Lipids


since 2012 Member of the Editorial Board of Cell Metabolism

since 2013 European Coordinator of the Consortium “Triglyceride Metabolism in

Obesity and Cardiovascular Disease”, Fondation Leducq, USA-France

2010 - 2016 Executive Editor: BBA-Molecular and Cellular Biology of Lipids

since 2008 Member of the Austrian Academy of Sciences

2007 - 2017 Speaker of the Research Consortium SFB LIPOTOX, Graz, Austria

Reviewer: Science, Nature, Cell, Nature Medicine, Cell metabolism

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54



Funding Org.

Project Number


Funding Period


F30

SFB LIPOTOX - Lipases and the Control of Energy Balance, Insulin

Resistance, and Lipotoxicity (Coordinator & PI) - 1st, 2nd and 3rd

funding period

4150 (13100 for


FWF Z136 Wittgenstein Award: Metabolic

Lipase in Energy and Lipid Metabolism

1500 2008-2014

European Research Council (ERC)

ERC-2013-ADG 340896

ERC Advanced Grant: The Role of Lipolysis in the Pathogenesis of

Cancer-associated Cachexia 2500 2014-2019

Foundation Leducq

12CVD04

Transatlantic Network on Triglycerides - Triglyceride Metabolism in Obesity and

Cardiovascular Disease (Coordinator & PI)

770 (US$ 6.000

for consortium)

2013-2019

Fondation Louis

Jeantet -

Louis-Jeantet Prize for Medicine 2015

625 2015-2019


Chuna Ram CHOUDHARY, NNF Center for Protein Research, University of Copenhagen,

Denmark

Stephen G. YOUNG, Departments of Medicine (Cardiology) and Human Genetics, David

Geffen School of Medicine, University of California, Los Angeles, CA, USA

Erin KERSHAW, Division of Endocrinology, Diabetes, and Metabolism University of Pittsburg,

PA, USA

Erwin WAGNER, CNIO Spanish National Cancer Research Center, Madrid, Spain

Patrick SCHRAUWEN and Mattheijs HESSELINK, Maastricht University, The Netherlands


M. CUERVO, Albert Einstein College of Medicine, Bronx, NY, US: Lipophagy in aging mice;

crosstalk between neutral lipolysis and lipophyagy.

D. LANGIN, Obesity Research Laboratory, CHU Rangueil, Toulouse, France: Lipolytic and

non-lipolytic functions of HSL furing aging.

U. KINTSCHER, Center for Cardiovascular Research, Inst. of Pharmacology, Charite -

Universitätsmedizin Berlin, Germany: Lipolysis in cardiac endothelial cells; cardiac function in

mouse models with defective lipolysis.

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[1] Kotzbeck P, Giordano A, Mondini E, Murano I, Severi I, Venema W, Cecchini MP, Kershaw EE, Barbatelli G, Haemmerle G, Zechner R, Cinti S. Brown adipose tissue whitening leads to brown adipocyte death and adipose tissue inflammation J Lipid Res 2018,. pii: jlr.M079665. DOI: 10.1194/jlr.M079665. [Epub ahead ofprint]

[2] Padmanabha Das KM, Wechselberger L, Liziczai M, De la Rosa Rodriguez M, Grabner GF, Heier C, Viertlmayr R, Radler C, Lichtenegger J, Zimmermann R, Borst JW, Zechner R, Kersten S, Oberer M. Hypoxia-inducible lipid droplet-associated protein inhibits adipose triglyceride lipase J Lipid Res 2018, 59(3), 531-541. DOI: 10.1194/jlr.M082388.

[3] Simcox J, Geoghegan G, Maschek JA, Bensard CL, Pasquali M, Miao R, Lee S, Jiang L, Huck I, Kershaw EE, Donato AJ, Apte U, Longo N, Rutter J, Schreiber R, Zechner R, Cox J, Villanueva CJ. Global Analysis of Plasma Lipids Identifies Liver-Derived Acylcarnitines as a Fuel Source for Brown Fat Thermogenesis Cell Metab 2017, 26(3), 509-522.e6. DOI: 10.1016/j.cmet.2017.08.006.

[4] Schreiber R, Diwoky C, Schoiswohl G, Feiler U, Wongsiriroj N, Abdellatif M, Kolb D, Hoeks J, Kershaw EE, Sedej S, Schrauwen P, Haemmerle G, Zechner R. Cold-Induced Thermogenesis Depends on ATGL-Mediated Lipolysis in Cardiac Muscle, but Not Brown Adipose Tissue Cell Metab 2017, 26(5), 753-763.e7. DOI:10.1016/j.cmet.2017.09.004.

[5] Heier C, Kien B, Huang F, Eichmann TO, Xie H, Zechner R, Chang PA. The phospholipase PNPLA7 functions as a lysophosphatidylcholine hydrolase and interacts with lipid droplets through its catalytic domain J Biol Chem 2017 292(46), 19087-19098. DOI: 10.1074/jbc.M117.792978.doi: 10.1074/jbc.M117.792978.

[6] Schweiger M, Romauch M, Schreiber R, Grabner GF, Hütter S, Kotzbeck P, Benedikt P, Eichmann TO, Yamada S, Knittelfelder O, Diwoky C, Doler C, Mayer N, De Cecco W, Breinbauer R, Zimmermann R, Zechner R. Pharmacological inhibition of adipose triglyceride lipase corrects high fat diet-induced obesity, insulin resistance, and liver steatosis in mice Nat Commun 2017, 22(8), 14859. DOI: 10.1038/ncomms14859

[7] Kory N, Grond S, Kamat SS, Li Z, Krahmer N, Chitraju C, Zhou P, Fröhlich F, Semova I, Ejsing C, Zechner R, Cravatt BF, Farese RV Jr, Walther TC. Mice lacking lipid droplet-associated hydrolase, a gene linked to human prostate cancer, have normal cholesterol ester metabolism J Lipid Res 2017, 58(1), 226-235. DOI: 10.1194/jlr.M072538

[8] Zechner R, Madeo F, Kratky D. Cytosolic lipolysis and lipophagy: two sides of the same coin Nat Rev Mol Cell Biol 2017, 18(11), 671-684. DOI: 10.1038/nrm.2017.76.

[9] Grond S, Eichmann TO, Dubrac S, Kolb D, Schmuth M, Fischer J, Crumrine D, Elias PM, Haemmerle G, Zechner R, Lass A, Radner FP. PNPLA1 Deficiency in Mice and Humans Leads to a Defect in the Synthesis of Omega-O-Acylceramides J Invest Dermatol 2017, 137(2), 394-402. DOI: 10.1016/j.jid.2016.08.036

[10] Grond S, Radner FP, Eichmann TO, Kolb D, Grabner GF, Wolinski H, Gruber R, Hofer P, Heier C, Schauer S, Rülicke T, Hoefler G, Schmuth M, Elias PM, Lass A, Zechner R, Haemmerle G. Skin Barrier Development Depends on CGI-58 Protein Expression during Late-Stage Keratinocyte Differentiation J Invest Dermatol 2017, 137(2), 403-413. DOI: 10.1016/j.jid.2016.09.025

[11] Heckmann BL, Zhang X, Saarinen AM, Schoiswohl G, Kershaw EE, Zechner R, Liu J. Liver X receptor alpha mediates hepatic triglyceride accumulation through upregulation of G0/G1 Switch Gene 2 expression JCI Insight 2017, 2(4), e88735. DOI: 10.1172/jci.insight.88735.

[12] Heier C, Taschler U, Radulovic M, Aschauer P, Eichmann TO, Grond S, Wolinski H, Oberer M, Zechner R, Kohlwein SD, Zimmermann R. Monoacylglycerol Lipases Act as

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Evolutionarily Conserved Regulators of Non-oxidative Ethanol Metabolism J Biol Chem 2016, 291(22), 11865-11875. DOI: 10.1074/jbc.M115.705541

[13] Al-Zoughbi W, Pichler M, Gorkiewicz G, Guertl-Lackner B, Haybaeck J, Jahn SW, Lackner C, Liegl-Atzwanger B, Popper H, Schauer S, Nusshold E, Kindt AS, Trajanoski Z, Speicher MR, Haemmerle G, Zimmermann R, Zechner R, Vesely PW, Hoefler G. Loss of adipose triglyceride lipase is associated with human cancer and induces mouse pulmonary neoplasia Oncotarget 2016, 7(23), 33832-33840. DOI: 10.18632/oncotarget.9418

[14] Grumet L, Eichmann TO, Taschler U, Zierler KA, Leopold C, Moustafa T, Radovic B, Romauch M, Yan C, Du H, Haemmerle G, Zechner R, Fickert P, Kratky D, Zimmermann R, Lass A. Lysosomal Acid Lipase Hydrolyzes Retinyl Ester and Affects Retinoid Turnover J Biol Chem 2016, 291(34), 17977-17987. DOI: 10.1074/jbc.M116.724054

[15] Lord CC, Ferguson D, Thomas G, Brown AL, Schugar RC, Burrows A, Gromovsky AD, Betters J, Neumann C, Sacks J, Marshall S, Watts R, Schweiger M, Lee RG, Crooke RM, Graham MJ, Lathia JD, Sakaguchi TF, Lehner R, Haemmerle G, Zechner R, Brown JM. Regulation of Hepatic Triacylglycerol Metabolism by CGI-58 Does Not Require ATGL Co-activation Cell Rep 2016, 16(4), 939-949. DOI: 10.1016/j.celrep.2016.06.049

[16] Schreiber R, Hofer P, Taschler U, Voshol PJ, Rechberger GN, Kotzbeck P, Jaeger D, Preiss-Landl K, Lord CC, Brown JM, Haemmerle G, Zimmermann R, Vidal-Puig A, Zechner R. Hypophagia and metabolic adaptations in mice with defective ATGL-mediated lipolysis cause resistance to HFD-induced obesity Proc Natl Acad Sci U S A 2015, 112(45), 13850-13855. DOI: 10.1073/pnas.1516004112

[17] Weinert BT, Iesmantavicius V, Moustafa T, Schölz C, Wagner SA, Magnes C, Zechner R, Choudhary C. Acetylation dynamics and stoichiometry in Saccharomyces cerevisiae Mol Syst Biol 2015, 11(10):833. DOI: 10.15252/msb.156513

[18] Weinert BT, Moustafa T, Iesmantavicius V, Zechner R, Choudhary C. Analysis of acetylation stoichiometry suggests that SIRT3 repairs nonenzymatic acetylation lesions EMBO J 2015, 34(21), 2620-2632. DOI: 10.15252/embj.201591271

[19] Boeszoermenyi A, Nagy HM, Arthanari H, Pillip CJ, Lindermuth H, Luna RE, Wagner G, Zechner R, Zangger K, Oberer M. Structure of a CGI-58 Motif Provides the Molecular Basis of Lipid Droplet Anchoring J Biol Chem 2015, 290(44), 26361-26372. DOI10.1074/jbc.M115.682203

[20] Heier C, Radner FP, Moustafa T, Schreiber R, Grond S, Eichmann TO, Schweiger M, Schmidt A, Cerk IK, Oberer M, Theussl HC, Wojciechowski J, Penninger JM, Zimmermann R, Zechner R. G0/G1 Switch Gene 2 Regulates Cardiac Lipolysis J Biol Chem 2015, 290(43), 26141-261450. DOI: 10.1074/jbc.M115.671842

[21] Eichmann TO, Grumet L, Taschler U, Hartler J, Heier C, Woblistin A, Pajed L, Kollroser M, Rechberger G, Thallinger GG, Zechner R, Haemmerle G, Zimmermann R, Lass A. ATGL and CGI-58 are lipid droplet proteins of the hepatic stellate cell line HSC-T6 J Lipid Res 2015, 56(10), 1972-1984. DOI: 10.1194/jlr.M062372

[22] Schweiger M, Zechner R. Breaking the Barrier-chaperone-mediated autophagy of perilipins regulates the lipolytic degradation of fat Cell Metab 2015, 22(1), 60-61.DOI: 10.1016/j.cmet.2015.06.017

[23] Hofer P, Boeszoermenyi A, Jaeger D, Feiler U, Arthanari H, Mayer N, Zehender F, Rechberger G, Oberer M, Zimmermann R, Lass A, Haemmerle G, Breinbauer R, Zechner R, Preiss-Landl K. Fatty Acid-binding Proteins Interact with Comparative Gene Identification-58 Linking Lipolysis with Lipid Ligand Shuttling J Biol Chem 2015, 290(30), 18438-18453. DOI: 10.1074/jbc.M114.628958

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[24] Das SK, Stadelmeyer E, Schauer S, Schwarz A, Strohmaier H, Claudel T, Zechner R, Hoefler G, Vesely PW. Micro RNA-124a regulates lipolysis via adipose triglyceride lipase and comparative gene identification 58 Int J Mol Sci 2015, 16(4), 8555-8568. DOI: 10.1016/j.jnutbio.2010.12.014

[25] Mayer N, Schweiger M, Melcher MC, Fledelius C, Zechner R, Zimmermann R, Breinbauer R. Structure-activity studies in the development of a hydrazone based inhibitor of adipose-triglyceride lipase (ATGL) Bioorg Med Chem 2015, 23(12), 2904-2916. DOI: 10.1016/j.bmc.2015.02.051

[26] Jaeger D, Schoiswohl G, Hofer P, Schreiber R, Schweiger M, Eichmann TO, Pollak NM, Poecher N, Grabner GF, Zierler KA, Eder S, Kolb D, Radner FP, Preiss-Landl K, Lass A, Zechner R, Kershaw EE, Haemmerle G. Fasting-induced G0/G1 switch gene 2 and FGF21 expression in the liver are under regulation of adipose tissue derived fatty acids J Hepatol 2015, 63(2), 437-445. DOI: 10.1016/j.jhep.2015.02.035

[27] Taschler U, Schreiber R, Chitraju C, Grabner GF, Romauch M, Wolinski H, Haemmerle G, Breinbauer R, Zechner R, Lass A, Zimmermann R. Adipose triglyceride lipase is involved in the mobilization of triglyceride and retinoid stores of hepatic stellate cells Biochim Biophys Acta 2015, 1851(7), 937-945. DOI: 10.1016/j.bbalip.2015.02.017

[28] Zechner R. FAT FLUX: enzymes, regulators, and pathophysiology of intracellular lipolysis EMBO Mol Med 2015, 7(4), 359-362. DOI: 10.15252/emmm.201404846

[29] Savonen R, Hiden M, Hultin M, Zechner R, Levak-Frank S, Olivecrona G, Olivecrona T. The tissue distribution of lipoprotein lipase determines where chylomicrons bind J Lipid Res 2015, 56(3):588-598. DOI: 10.1194/jlr.M056028

[30] Folick A, Oakley HD, Yu Y, Armstrong EH, Kumari M, Sanor L, Moore DD, Ortlund EA, Zechner R, Wang MC. Aging. Lysosomal signaling molecules regulate longevity in Caenorhabditis elegans Science 2015, 347(6217), 83-86. DOI: 10.1126/science.1258857

[31] Pollak NM, Jaeger D, Kolleritsch S, Zimmermann R, Zechner R, Lass A, Haemmerle G. The interplay of protein kinase A and perilipin 5 regulates cardiac lipolysis J Biol Chem 2015, 290(3), 1295-1306. DOI: 10.1074/jbc.M114.604744

[32] Camus G, Schweiger M, Herker E, Harris C, Kondratowicz AS, Tsou CL, Farese RV Jr, Herath K, Previs SF, Roddy TP, Pinto S, Zechner R, Ott M. The hepatitis C virus core protein inhibits adipose triglyceride lipase (ATGL)-mediated lipid mobilization and enhances the ATGL interaction with comparative gene identification 58 (CGI-58) and lipid droplets J Biol Chem 2014, 289(52), 35770-35780. DOI: 10.1074/jbc.M114.587816

[33] Schreiber R, Zechner R. Lipolysis meets inflammation: arachidonic acid mobilization from fat J Lipid Res 2014, 55(12), 2447-2449. DOI: 10.1194/jlr.C055673

[34] Mussbacher M, Stessel H, Wölkart G, Haemmerle G, Zechner R, Mayer B, Schrammel A. Role of the ubiquitin-proteasome system in cardiac dysfunction of adipose triglyceride lipase-deficient mice J Mol Cell Cardiol 2014, 77, 11-19. DOI: 10.1016/j.yjmcc.2014.09.028

[35] Cerk IK, Salzburger B, Boeszoermenyi A, Heier C, Pillip C, Romauch M, Schweiger M, Cornaciu I, Lass A, Zimmermann R, Zechner R, Oberer M. A peptide derived from G0/G1 switch gene 2 acts as noncompetitive inhibitor of adipose triglyceride lipase J Biol Chem 2014, 289(47), 32559-32570. DOI: 10.1074/jbc.M114.602599

[36] Brahma MK, Adam RC, Pollak NM, Jaeger D, Zierler KA, Pöcher N, Schreiber R, Romauch M, Moustafa T, Eder S, Ruelicke T, Preiss-Landl K, Lass A, Zechner R, Haemmerle G. Fibroblast growth factor 21 is induced upon cardiac stress and alters cardiac lipid homeostasis J Lipid Res 2014, 55(11), 2229-2241. DOI: 10.1194/jlr.M044784

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[37] Zechner R, Langin D. Hormone-sensitive lipase deficiency in humans Cell Metab 2014, 20(2), 199-201. DOI: 10.1016/j.cmet.2014.07.018

[38] Petruzzelli M, Schweiger M, Schreiber R, Campos-Olivas R, Tsoli M, Allen J, Swarbrick M, Rose-John S, Rincon M, Robertson G, Zechner R, Wagner EF. A switch from white to brown fat increases energy expenditure in cancer-associated cachexia Cell Metab 2014, 20(3), 433-447. DOI: 10.1016/j.cmet.2014.06.011

[39] Mattijssen F, Georgiadi A, Andasarie T, Szalowska E, Zota A, Krones-Herzig A, Heier C, Ratman D, De Bosscher K, Qi L, Zechner R, Herzig S, Kersten S. Hypoxia inducible lipid droplet associated (HILPDA) is a novel peroxisome proliferator-activated receptor (PPAR) target involved in hepatic triglyceride secretion J Biol Chem 2014, 11, 289(28), 19279-93. DOI: 10.1074/jbc.M114.570044

[40] Tsoli M, Schweiger M, Vanniasinghe AS, Painter A, Zechner R, Clarke S, Robertson G. Depletion of white adipose tissue in cancer cachexia syndrome is associated with inflammatory signaling and disrupted circadian regulation PLoS ONE 2014, 9(3), e92966. DOI: 10.1371/journal.pone.0092966

[41] Schrammel A, Mussbacher M, Wölkart G, Stessel H, Pail K, Winkler S, Schweiger M, Haemmerle G, Al Zoughbi W, Höfler G, Lametschwandtner A, Zechner R, Mayer B. Endothelial dysfunction in adipose triglyceride lipase deficiency Biochim Biophys Acta 2014, 1841(6), 906-917. DOI: 10.1016/j.bbalip.2014.03.005

[42] Kotzbeck P, Zechner R. Angiopoietin-like 4: An endogenous break of intestinal lipid digestion Mol Metab 2014, 3(2), 88-89. DOI: 10.1016/j.molmet.2014.01.009

[43] Grahn TH, Kaur R, Yin J, Schweiger M, Sharma VM, Lee MJ, Ido Y, Smas CM, Zechner R, Lass A, Puri V. Fat-specific Protein 27 (FSP27) Interacts with Adipose Triglyceride Lipase (ATGL) to Regulate Lipolysis and Insulin Sensitivity in Human Adipocytes J Biol Chem 2014, 289(17), 12029-12039. DOI: 10.1074/jbc.M113.539890

[44] Zierler KA, Zechner R, Haemmerle G. Comparative gene identification-58/α/β hydrolase domain 5: more than just an adipose triglyceride lipase activator? Curr Opin Lipidol 2014, 25(2), 102-109. DOI: 10.1097/MOL.0000000000000058

[45] Schweiger M, Eichmann TO, Taschler U, Zimmermann R, Zechner R, Lass A. Measurement of lipolysis Methods Enzymol 2014, 538, 171-193. DOI: 10.1016/B978-0-12-800280-3.00010-4

[46] Weinert BT, Iesmantavicius V, Moustafa T, Schölz C, Wagner SA, Magnes C, Zechner R, Choudhary C. Acetylation dynamics and stoichiometry in Saccharomyces cerevisiae Mol Syst Biol 2014, 10, 716. DOI: 10.15252/msb.156513

[47 Nagy HM, Paar M, Heier C, Moustafa T, Hofer P, Haemmerle G, Lass A, Zechner R, Oberer M, Zimmermann R. Adipose triglyceride lipase activity is inhibited by long-chain acyl-coenzyme A Biochim Biophys Acta 2014, 1841(4), 588-594. DOI: 10.1016/j.bbalip.2014.01.005

[48] Mayer N, Schweiger M, Romauch M, Grabner GF, Eichmann TO, Fuchs E, Ivkovic J, Heier C, Mrak I, Lass A, Höfler G, Fledelius C, Zechner R, Zimmermann R, Breinbauer R. Development of small-molecule inhibitors targeting adipose triglyceride lipase Nat Chem Biol 2013, 9(12), 785-787. DOI: 10.1038/nchembio.1359

[49] Jha P, Claudel T, Baghdasaryan A, Mueller M, Halilbasic E, Das SK, Lass A, Zimmermann R, Zechner R, Hoefler G, Trauner M. Role of adipose triglyceride lipase (PNPLA2) in protection from hepatic inflammation in mouse models of steatohepatitis and endotoxemia Hepatology 2014, 59(3), 858-869. DOI: 10.1002/hep.26732

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[50] Schrammel A, Mussbacher M, Winkler S, Haemmerle G, Stessel H, Wölkart G, Zechner R, Mayer B. Cardiac oxidative stress in a mouse model of neutral lipid storage disease Biochim Biophys Acta 2013, 1831(11), 1600-1608. DOI: 10.1016/j.bbalip.2013.07.004

[51] Zierler KA, Jaeger D, Pollak NM, Eder S, Rechberger GN, Radner FP, Woelkart G, Kolb D, Schmidt A, Kumari M, Preiss-Landl K, Pieske B, Mayer B, Zimmermann R, Lass A, Zechner R, Haemmerle G. Functional cardiac lipolysis in mice critically depends on comparative gene identification-58 J Biol Chem 2013, 288(14), 9892-9904. DOI: 10.1074/jbc.M112.420620

[52] Sitnick MT, Basantani MK, Cai L, Schoiswohl G, Yazbeck CF, Distefano G, Ritov V, DeLany JP, Schreiber R, Stolz DB, Gardner NP, Kienesberger PC, Pulinilkunnil T, Zechner R, Goodpaster BH, Coen P, Kershaw EE. Skeletal muscle triacylglycerol hydrolysis does not influence metabolic complications of obesity Diabetes 2013, 62(10), 3350-3361. DOI: 10.2337/db13-0500

[53] Currie E, Schulze A, Zechner R, Walther TC, Farese RV Jr. Cellular fatty acid metabolism and cancer Cell Metab 2013, 18(2), 153-161. DOI: 10.1016/j.cmet.2013.05.017

[54] Chitraju C, Trötzmüller M, Hartler J, Wolinski H, Thallinger GG, Haemmerle G, Zechner R, Zimmermann R, Köfeler HC, Spener F. The impact of genetic stress by ATGL deficiency on the lipidome of lipid droplets from murine hepatocytes J Lipid Res 2013, 54(8), 2185-2194. DOI: 10.1194/jlr.M037952

[55] Kienesberger PC, Pulinilkunnil T, Nagendran J, Young ME, Bogner-Strauss JG, Hackl H, Khadour R, Heydari E, Haemmerle G, Zechner R, Kershaw EE, Dyck JR. Early structural and metabolic cardiac remodelling in response to inducible adipose triglyceride lipase ablation Cardiovasc Res 2013, 99(3), 442-451. DOI: 10.1093/cvr/cvt124

[56] Young SG, Zechner R. Biochemistry and pathophysiology of intravascular and intracellular lipolysis Genes Dev 2013, 27(5):459-484. DOI: 10.1101/gad.209296.112

[57] Pollak NM, Schweiger M, Jaeger D, Kolb D, Kumari M, Schreiber R, Kolleritsch S, Markolin P, Grabner GF, Heier C, Zierler KA, Rülicke T, Zimmermann R, Lass A, Zechner R, Haemmerle G. Cardiac-specific overexpression of perilipin 5 provokes severe cardiac steatosis via the formation of a lipolytic barrier J Lipid Res 2013, 54(4), 1092-1102. DOI: 10.1194/jlr.M034710

[58] Pulinilkunnil T, Kienesberger PC, Nagendran J, Waller TJ, Young ME, Kershaw EE, Korbutt G, Haemmerle G, Zechner R, Dyck JR. 2013. Myocardial Adipose Triglyceride Lipase Overexpression Protects Diabetic Mice From the Development of Lipotoxic Cardiomyopathy Diabetes 2013, 62(5), 1464-1477. DOI: 10.2337/db12-0927

[59] van de Weijer T, Havekes B, Bilet L, Hoeks J, Sparks L, Bosma M, Paglialunga S, Jorgensen J, Janssen MC, Schaart G, Sauerwein H, Smeets JL, Wildberger J, Zechner R, Schrauwen-Hinderling VB, Hesselink MK, Schrauwen P. Effects of Bezafibrate Treatment in a Patient and a Carrier With Mutations in the PNPLA2 Gene, Causing Neutral Lipid Storage Disease With Myopathy Circ Res 2013, 112(5), e51-54. DOI: 10.1161/CIRCRESAHA.113.300944

[60] Yasmeen R, Reichert B, Deiuliis J, Yang F, Lynch A, Meyers J, Sharlach M, Shin S, Volz KS, Green KB, Lee K, Alder H, Duester G, Zechner R, Rajagopalan S, Ziouzenkova O. Autocrine function of aldehyde dehydrogenase 1 as a determinant of diet- and sex-specific differences in visceral adiposity Diabetes 2013, 62(1), 124-136. DOI: 10.2337/db11-1779.

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[1] Schweiger M, Romauch M, Schreiber R, Grabner GF, Hütter S, Kotzbeck P, Benedikt P, Eichmann TO, Yamada S, Knittelfelder O, Diwoky C, Doler C, Mayer N, De Cecco W, Breinbauer R, Zimmermann R, Zechner R. Pharmacological inhibition of adipose triglyceride lipase corrects high fat diet-induced obesity, insulin resistance, and liver steatosis in mice Nat Commun 2017, 22(8),14859. DOI: 10.1038/ncomms14859

[2] Schreiber R, Hofer P, Taschler U, Voshol PJ, Rechberger GN, Kotzbeck P, Jaeger D, Preiss-Landl K, Lord CC, Brown JM, Haemmerle G, Zimmermann R, Vidal-Puig A, Zechner R. Hypophagia and metabolic adaptations in mice with defective ATGL-mediated lipolysis cause resistance to HFD-induced obesity Proc Natl Acad Sci USA 2015, 112(45), 13850-13855. DOI: 10.1073/pnas.1516004112

[3] Heier C, Radner FP, Moustafa T, Schreiber R, Grond S, Eichmann TO, Schweiger M, Schmidt A, Cerk IK, Oberer M, Theussl HC, Wojciechowski J, Penninger JM, Zimmermann R, Zechner R. G0/G1 Switch Gene 2 Regulates Cardiac Lipolysis J Biol Chem 2015, 290(43), 26141-26150. DOI: 10.1074/jbc.M115.671842

[4] Mayer N, Schweiger M, Romauch M, Grabner GF, Eichmann TO, Fuchs E, Ivkovic J, Heier C, Mrak I, Lass A, Höfler G, Fledelius C, Zechner R, Zimmermann R, Breinbauer R. Development of small-molecule inhibitors targeting adipose triglyceride lipase Nat Chem Biol 2013, 9(12), 785-787. DOI: 10.1038/nchembio.1359

[5] Kumari M, Schoiswohl G, Chitraju C, Paar M, Cornaciu I, Rangrez AY, Wongsiriroj N, Nagy HM, Ivanova PT, Scott SA, Knittelfelder O, Rechberger GN, Birner-Gruenberger R, Eder S, Brown HA, Haemmerle G, Oberer M, Lass A, Kershaw EE, Zimmermann R, Zechner R. Adiponutrin functions as a nutritionally regulated lysophosphatidic acid acyltransferase Cell Metab 2012, 15(5), 691-702. DOI: 10.1016/j.cmet.2012.04.008

[6] Haemmerle G, Moustafa T, Woelkart G, Kotzbeck P, Büttner S, Schmidt A, van de Weijer T, Hesselink M, Jäger D, Kienesberger P, Zierler K, Schreiber R, Eichmann T, Kolb D, Schweiger M, Kumari M, Eder S, Schoiswohl G, Wongsiriroj N, Pollak N, Radner FW, Preiss-Landl K, Kolbe T, Rülicke T, Pieske B, Trauner M, Lass A, Zimmermann R, Hoefler G, Cinti S, Kershaw EE, Schrauwen P, Madeo F, Mayer B, Zechner R. ATGL-mediated fat catabolism regulates cardiac mitochondrial function via the PPARa and PGC-1a and b Nat Med 2011, 17(9), 1076-1085. DOI: 10.1038/nm.2439

[7] Das SK, Eder S, Schauer S, Diwoky C, Temmel H, Guertl B, Gorkiewicz G, Tamilarasan KP, Kumari P, Trauner M, Zimmermann R, Vesely P, Haemmerle G, Zechner R, Hoefler G. Adipose triglyceride lipase contributes to cancer-associated cachexia Science 2011, 333(6039), 233-238. DOI: 10.1126/science.1198973

[8] Haemmerle G, Lass A, Zimmermann R, Gorkiewicz G, Meyer C, Rozman J, Heldmaier G, Maier R, Theussl C, Eder S, Kratky D, Wagner EF, Klingenspor M, Hoefler G, Zechner R. Defective lipolysis and altered energy metabolism in mice lacking adipose triglyceride lipase Science 2006, 312(5774), 734-737. DOI: 10.1126/science.1123965

[9] Lass A, Zimmermann R, Haemmerle G, Riederer M, Schoiswohl G, Schweiger M, Kienesberger P, Strauss JG, Gorkiewicz G, Zechner R. Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome Cell Metab 2006, 3(5), 309-319. DOI: 10.1016/j.cmet.2006.03.005

[10] Zimmermann R, Strauss JG, Haemmerle G, Schoiswohl G, Birner-Gruenberger R, Riederer M, Lass A, Neuberger G, Eisenhaber F, Hermetter A, Zechner R. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase Science 2004, 306(5700), 1383-1386. DOI: 10.1126/science.1100747

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Patents

Zimmermann R, Zechner R, Haemmerle G, Hoefler G, Das S, Lass A. 2015. Method for

treatment of cachexia by administering inhibitors of adipose triglyceride lipase expression or

activity. WO2010115825A2

Hoefler G, Zechner R, Zimmermann R, Haemmerle G, Lass A, Das S. 2016. Modulating the

activity of triglyceride hydrolase. EP 1765997 B1

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Curriculum Vitae – Assoz. Prof. Dr. rer. nat. Robert Zimmermann Institute of Molecular Biosciences, University of Graz, Heinrichstrasse 31, 3rd floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 1900 (1914), E-Mail: [email protected] https://molekularbiologie.uni-graz.at/en/research/areas-of-research/energy-and-lipid-metabolism/

Year of Birth *1967


Orcid ID https://orcid.org/0000-0002-7354-870X




Keywords lipid metabolism, lipid-metabolizing enzymes, lipid signaling, mutant

animal models


since 2010 Associate professor, Institute of Molecular Biosciences, University of

Graz, Austria

2007 Habilitation in “Molecular Biology and Biochemistry”

2002 - 2010 Assistant professor at the Institute of Molecular Biosciences, University of

Graz

1998 - 2002 Postdoc at the Institute of Biochemistry, University of Graz

Education

1998 Graduation “Dr. rer. nat.”, at the University of Graz

1994 - 1998 Ph.D. thesis at the Inst. of Medical Biochemistry, University of Graz

1994 Graduation “Master of Science”, University of Graz

1986 Studies in Biology, University of Graz, Austria


2016 “α/β Hydrolase domain-containig 6 degrades the late

endosomal/lysosomal lipid Bis(monoacylglycero) phosphate”. 84th EAS

Congress, Innsbruck, Austria.

2013 “Adipose triglyceride lipase: Role in energy metabolism and implications

for metabolic disorders” 40th Anniversary UK Adipose Tissue Discussion

Group December 12-13th 2013, Edinburgh


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2012 “Monoglyceride Lipase at the crossroads of lipid metabolism and

endocannabinoid signaling”, Congress: Lipid Droplets: Metabolic

Consequences of the Storage of Neutral Lipids (FASEB), Snowmass,

Colorado (USA), 07/22/2012.

2011 “Role of neutral lipases in glucose and lipid metabolism” ÖDG

Jahrestagung 2011, Salzburg, Austria.

2011 “Adipose triglyceride lipase: Role in energy metabolism and implications

for metabolic disorders”, Congress: Triglyceride Deposit

Cardiomyovasculopathy and Neutral Lipid Storage Disease, Kyoto,

Japan, 11/26/2011.

Career Related Activities in Editorial Boards, as Reviewers and Memberships

Reviewer of peer-reviewed Journals (Cell Metabolism, BBA, JBC, JLR, etc.)

Memberships: International Cannabinoid Research Society (ICRS), Austrian Atherosclerosis

Society (AAS), The American Society for Biochemistry and Molecular Biology (ASBMB).



Funding Org.

Project Number


Funding Period

FFG 859212 Preclinical Development of small

molecule inhibitors targeting human Adipose Triglyceride Lipase

688 (1.2 for


FWF P 28533 Molecular mechanisms mediating

ZAG signaling 450 2016-2019

FWF P 26166 Functional characterization of

α/β hydrolase domain-containing 6 (ABHD6)

450 2014-2017



559 2011-2019

FWF P24294 Characterization of mice with tissue

-specific gene deletion of monoglyceride lipase

300 2012-2015


Matthias TSCHÖP, Institute for Diabetes and Obesity, Helmholtz Zentrum München, Germany

Jarmo T. LAITINEN, University of Eastern Finland, Institute of Biomedicine, Kuopio, Finland

Jonathan M. BROWN, Department of Cellular and Molecular Medicine, Cleveland Clinic Lerner

Research Institute, Cleveland, OH, USA

Judith FISCHER, University Medical Center Freiburg, Freiburg, Germany

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Siamak BAHRAM, Strasbourg University School of Medcine and Teaching Hospitals,

Strasbourg, France


Jonathan M. BROWN, Department of Cellular and Molecular Medicine, Cleveland Clinic Lerner

Research Institute, Cleveland, OH, USA: Pathophysiology of α/β hydrolases;

Jarmo T. LAITINEN, University of Eastern Finland, Finland: Endocannabinoid metabolism and

action.

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[1] Padmanabha Das KM., Wechselberger L., Liziczai M., De la Rosa Rodriguez M., Grabner GF., Heier C., Viertlmayr R., Radler C., Lichtenegger J., Zimmermann R., Borst JW., Zechner R., Kersten S., Oberer M. Hypoxia Inducible Lipid Droplet Associated protein inhibits Adipose Triglyceride Lipase J Lipid Res 2018, DOI: 10.1194/jlr.M082388.

[2] Jarc E., Kump A., Malavašič P., Eichmann TO., Zimmermann R., Petan T. Lipid droplets induced by secreted phospholipase A 2 and unsaturated fatty acids protect breast cancer cells from nutrient and lipotoxic stress Biochim Biophys Acta - Mol Cell Biol Lipids 2018, 1863, 247–265. DOI: 10.1016/j.bbalip.2017.12.006.

[3] Curcic S., Holzer M., Pasterk L., Knuplez E., Eichmann TO., Frank S., Zimmermann R., Schicho R., Heinemann A., Marsche G. Secretory phospholipase A2 modified HDL rapidly and potently suppresses platelet activation Sci Rep 2017, 7, 8030. DOI: 10.1038/s41598-017-08136-1.

[4] Grabner GF., Zimmermann R., Schicho R., Taschler U. Monoglyceride lipase as a drug target: At the crossroads of arachidonic acid metabolism and endocannabinoid signaling Pharmacol Ther 2017, 175, 35–46. DOI: 10.1016/j.pharmthera.2017.02.033.

[5] Schweiger M., Romauch M., Schreiber R., Grabner GF., Hütter S., Kotzbeck P., Benedikt P., Eichmann TO., Yamada S., Knittelfelder O., Diwoky C., Doler C., Mayer N., De Cecco W., Breinbauer R., Zimmermann R., Zechner R. Pharmacological inhibition of adipose triglyceride lipase corrects high-fat diet-induced insulin resistance and hepatosteatosis in mice Nat Commun 2017, 8, 14859. DOI: 10.1038/ncomms14859.

[6] Vujic N., Korbelius M., Leopold C., Duta-Mare M., Rainer S., Schlager S., Goeritzer M., Kolb D., Eichmann TO., Diwoky C., Zimmer A., Zimmermann R., Lass A., Radovic B., Kratky D. Monoglyceride lipase deficiency affects hepatic cholesterol metabolism and lipid-dependent gut transit in ApoE-/- mice Oncotarget 2017, 8, 33122–33136. DOI: 10.18632/oncotarget.16529.

[7] Heier C., Xie H., Zimmermann R. Nonoxidative ethanol metabolism in humans-from biomarkers to bioactive lipids IUBMB Life 2016, 68, 916–23. doi: 10.1002/iub.1569.

[8] Grumet L., Eichmann TO., Taschler U., Zierler KA., Leopold C., Moustafa T., Radovic B., Romauch M., Yan C., Du H., Haemmerle G., Zechner R., Fickert P., Kratky D., Zimmermann R., Lass A. Lysosomal Acid Lipase Hydrolyzes Retinyl Ester and Affects Retinoid Turnover J Biol Chem 2016, 291, 17977–17987 DOI: 10.1074/jbc.M116.724054.

[9] Al-Zoughbi W., Pichler M., Gorkiewicz G., Guertl-Lackner B., Haybaeck J., Jahn SW., Lackner C., Liegl-Atzwanger B., Popper H., Schauer S., Nusshold E., Kindt ASD., Trajanoski Z., Speicher MR., Haemmerle G., Zimmermann R., Zechner R., Vesely PW., Hoefler G. Loss of Adipose Triglyceride Lipase is associated with human cancer and induces mouse pulmonary neoplasia Oncotarget 2014, 7, 33832–33840. DOI: 10.18632/oncotarget.9418.

[10] Heier C., Taschler U., Radulovic M., Aschauer P., Eichmann TO., Grond S., Wolinski H., Oberer M., Zechner R., Kohlwein SD., Zimmermann R. Monoacylglycerol Lipases Act as Evolutionarily Conserved Regulators of Non-oxidative Ethanol Metabolism J Biol Chem 2016, 291, 11865–71185. DOI: 10.1074/jbc.M115.705541.

[11] Aschauer P., Rengachari S., Lichtenegger J., Schittmayer M., Das KMP., Mayer N., Breinbauer R., Birner-Gruenberger R., Gruber CC., Zimmermann R., Gruber K., Oberer M. Crystal structure of the Saccharomyces cerevisiae monoglyceride lipase Yju3p Biochim Biophys Acta 2016, 1861, 462–470. DOI: 10.1016/j.bbalip.2016.02.005.

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[12] Doler C., Schweiger M., Zimmermann R., Breinbauer R. Chemical Genetic Approaches for the Investigation of Neutral Lipid Metabolism Chembiochem 2016, 17, 358–377. DOI: 10.1002/cbic.201500501.

[13] Grabner GF., Eichmann TO., Wagner B., Gao Y., Farzi A., Taschler U., Radner FPW., Schweiger M., Lass A., Holzer P., Zinser E., Tschöp MH., Yi C-X., Zimmermann R. Deletion of Monoglyceride Lipase in Astrocytes Attenuates Lipopolysaccharide-induced Neuroinflammation J Biol Chem 2016, 291, 913–923. DOI: 10.1074/jbc.M115.683615.

[14] Vujic N., Schlager S., Eichmann TO., Madreiter-Sokolowski CT., Goeritzer M., Rainer S., Schauer S., Rosenberger A., Woelfler A., Doddapattar P., Zimmermann R., Hoefler G., Lass A., Graier WF., Radovic B., Kratky D. Monoglyceride lipase deficiency modulates endocannabinoid signaling and improves plaque stability in ApoE-knockout mice Atherosclerosis 2016, 244. DOI: 10.1016/j.atherosclerosis.2015.10.109.

[15] Pribasnig MA., Mrak I., Grabner GF., Taschler U., Knittelfelder O., Scherz B., Eichmann TO., Heier C., Grumet L., Kowaliuk J., Romauch M., Holler S., Anderl F., Wolinski H., Lass A., Breinbauer R., Marsche G., Brown JM., Zimmermann R. α/β Hydrolase Domain-containing 6 (ABHD6) Degrades the Late Endosomal/Lysosomal Lipid Bis(monoacylglycero)phosphate J Biol Chem 2015, 290, 29869–29881. DOI: 10.1074/jbc.M115.669168.

[16] Schreiber R., Hofer P., Taschler U., Voshol PJ., Rechberger GN., Kotzbeck P., Jaeger D., Preiss-Landl K., Lord CC., Brown JM., Haemmerle G., Zimmermann R., Vidal-Puig A., Zechner R. Hypophagia and metabolic adaptations in mice with defective ATGL-mediated lipolysis cause resistance to HFD-induced obesity Proc Natl Acad Sci U S A 2015, 112, 13850–1385 DOI i: 10.1073/pnas.1516004112.

[17] Heier C., Radner FPW., Moustafa T., Schreiber R., Grond S., Eichmann TO., Schweiger M., Schmidt A., Cerk IK., Oberer M., Theussl H-C., Wojciechowski J., Penninger JM., Zimmermann R., Zechner R. G0/G1 Switch Gene 2 Regulates Cardiac Lipolysis J Biol Chem 2015, 290, 26141–26150 DOI: 10.1074/jbc.M115.671842.

[18] Eichmann TO., Grumet L., Taschler U., Hartler J., Heier C., Woblistin A., Pajed L., Kollroser M., Rechberger G., Thallinger GG., Zechner R., Haemmerle G., Zimmermann R., Lass A. ATGL and CGI-58 are lipid droplet proteins of the hepatic stellate cell line HSC-T6 J Lipid Res 2015, 56, 1972–1984. DOI: 10.1194/jlr.M062372.

[19] Taschler U., Eichmann TO., Radner FPW., Grabner GF., Wolinski H., Storr M., Lass A., Schicho R., Zimmermann R. Monoglyceride lipase deficiency causes desensitization of intestinal cannabinoid receptor type 1 and increased colonic μ-opioid receptor sensitivity Br J Pharmacol 2015, 172, 4419–29 DOI: 10.1111/bph.13224.

[20] Hofer P., Boeszoermenyi A., Jaeger D., Feiler U., Arthanari H., Mayer N., Zehender F., Rechberger G., Oberer M., Zimmermann R., Lass A., Haemmerle G., Breinbauer R., Zechner R., Preiss-Landl K. Fatty Acid-binding Proteins Interact with Comparative Gene Identification-58 Linking Lipolysis with Lipid Ligand Shuttling J Biol Chem 2015, 290, 18438–18453 DOI: 10.1074/jbc.M114.628958.

[21] Taschler U., Schreiber R., Chitraju C., Grabner GF., Romauch M., Wolinski H., Haemmerle G., Breinbauer R., Zechner R., Lass A., Zimmermann R. Adipose triglyceride lipase is involved in the mobilization of triglyceride and retinoid stores of hepatic stellate cells Biochim Biophys Acta 2015, 1851, 937–945. DOI: 10.1016/j.bbalip.2015.02.017.

[22] Navia-Paldanius D., Aaltonen N., Lehtonen M., Savinainen JR., Taschler U., Radner FPW., Zimmermann R., Laitinen JT. Increased tonic cannabinoid CB1R activity and brain region-specific desensitization of CB1R Gi/o signaling axis in mice with global genetic knockout of monoacylglycerol lipase Eur J Pharm Sci 2015, 77, 180–188. DOI: 10.1016/j.ejps.2015.06.005.

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[23] Mayer N., Schweiger M., Melcher M-C., Fledelius C., Zechner R., Zimmermann R., Breinbauer R. Structure-activity studies in the development of a hydrazone based inhibitor of adipose-triglyceride lipase (ATGL) Bioorg Med Chem 2015, 23, 2904–2916 DOI: 10.1016/j.bmc.2015.02.051.

[24] Pollak NM., Jaeger D., Kolleritsch S., Zimmermann R., Zechner R., Lass A., Haemmerle G. The interplay of protein kinase A and perilipin 5 regulates cardiac lipolysis J Biol Chem 2015, 290, 1295–1306 DOI: 10.1074/jbc.M114.604744.

[25] Heier C., Zimmermann R. The sparing use of fat: G0s2 controls lipolysis and fatty acid oxidation Diabetologia 2015, 58, 7–9 DOI: 10.1007/s00125-014-3430-6.

[26] Cerk IK., Salzburger B., Boeszoermenyi A., Heier C., Pillip C., Romauch M., Schweiger M., Cornaciu I., Lass A., Zimmermann R., Zechner R., Oberer M. A peptide derived from G0/G1 switch gene 2 acts as noncompetitive inhibitor of adipose triglyceride lipase J Biol Chem 2014, 289, 32559–70. doi: 10.1074/jbc.M114.602599.

[27] Nagy HM., Paar M., Heier C., Moustafa T., Hofer P., Haemmerle G., Lass A., Zechner R., Oberer M., Zimmermann R. Adipose triglyceride lipase activity is inhibited by long-chain acyl-coenzyme A Biochim Biophys Acta 2014, 1841, 588–594 DOI: 10.1016/j.bbalip.2014.01.005.

[28] Jha P., Claudel T., Baghdasaryan A., Mueller M., Halilbasic E., Das SK., Lass A., Zimmermann R., Zechner R., Hoefler G., Trauner M. Role of adipose triglyceride lipase (PNPLA2) in protection from hepatic inflammation in mouse models of steatohepatitis and endotoxemia Hepatology 2014, 59, 858–869 DOI: 10.1002/hep.26732.

[29] Schweiger M., Eichmann TO., Taschler U., Zimmermann R., Zechner R., Lass A. Measurement of lipolysis Methods Enzym 2014, 538, 171–193 DOI: 10.1016/B978-0-12-800280-3.00010-4.

[30] Mayer N., Schweiger M., Romauch M., Grabner GF., Eichmann TO., Fuchs E., Ivkovic J., Heier C., Mrak I., Lass A., Hofler G., Fledelius C., Zechner R., Zimmermann R., Breinbauer R. Development of small-molecule inhibitors targeting adipose triglyceride lipase Nat Chem Biol 2013, 9, 785–787 DOI: 10.1038/nchembio.1359.

[31] Thomas G., Betters JL., Lord CC., Brown AL., Marshall S., Ferguson D., Sawyer J., Davis MA., Melchior JT., Blume LC., Howlett AC., Ivanova PT., Milne SB., Myers DS., Mrak I., Leber V., Heier C., Taschler U., Blankman JL., et al. The serine hydrolase ABHD6 Is a critical regulator of the metabolic syndrome Cell Rep 2013, 5, 508–520 DOI: 10.1016/j.celrep.2013.08.047.

[32] Alsted TJ., Ploug T., Prats C., Serup AK., Høeg L., Schjerling P., Holm C., Zimmermann R., Fledelius C., Galbo H., Kiens B. Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle J Physiol 2013, 591, 5141–5155 DOI: 10.1113/jphysiol.2013.260794.

[33] Chitraju C., Trötzmüller M., Hartler J., Wolinski H., Thallinger GG., Haemmerle G., Zechner R., Zimmermann R., Köfeler HC., Spener F. The impact of genetic stress by ATGL deficiency on the lipidome of lipid droplets from murine hepatocytes J Lipid Res 2013, 54, 2185–2194 DOI: 10.1194/jlr.M037952.

[34] Janssen MCH., van Engelen B., Kapusta L., Lammens M., van Dijk M., Fischer J., van der Graaf M., Wevers RA., Fahrleitner M., Zimmermann R., Morava E. Symptomatic lipid storage in carriers for the PNPLA2 gene Eur J Hum Genet 2013, 21, 807–815 DOI: 10.1038/ejhg.2012.256.

[35] Zierler KA., Jaeger D., Pollak NM., Eder S., Rechberger GN., Radner FPW., Woelkart G., Kolb D., Schmidt A., Kumari M., Preiss-Landl K., Pieske B., Mayer B., Zimmermann R., Lass A., Zechner R., Haemmerle G. Functional cardiac lipolysis in mice critically depends

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on comparative gene identification-58 J Biol Chem 2013, 288, 9892–9904 DOI: 10.1074/jbc.M112.420620.

[36] Pollak NM., Schweiger M., Jaeger D., Kolb D., Kumari M., Schreiber R., Kolleritsch S., Markolin P., Grabner GF., Heier C., Zierler KA., Rülicke T., Zimmermann R., Lass A., Zechner R., Haemmerle G. Cardiac-specific overexpression of perilipin 5 provokes severe cardiac steatosis via the formation of a lipolytic barrier J Lipid Res 2013, 54, 1092–1102. DOI 10.1194/jlr.M034710.


[1] Pribasnig, M. A., Mrak, I., Grabner, G. F., Taschler, U., Knittelfelder, O., Scherz, B., Eichmann, T. O., Heier, C., Grumet, L., Kowaliuk, J., Romauch, M., Holler, S., Anderl, F., Wolinski, H., Lass, A., Breinbauer, R., Marsche, G., Brown, J. M., and Zimmermann, R. α/β Hydrolase Domain-containing 6 (ABHD6) Degrades the Late Endosomal/Lysosomal Lipid Bis(monoacylglycero)phosphate J Biol Chem 2015, 290, 29869–29881. DOI: 10.1074/jbc.M115.669168

[2] Grabner, G. F., Eichmann, T. O., Wagner, B., Gao, Y., Farzi, A., Taschler, U., Radner, F. P. W., Schweiger, M., Lass, A., Holzer, P., Zinser, E., Tschop, M. H., Yi, C.-X., and Zimmermann, R. Deletion of Monoglyceride Lipase in Astrocytes Attenuates Lipopolysaccharide-Induced Neuroinflammation J Biol Chem 2015, 291, 913–923. DOI: 10.1074/jbc.M115.683615

[3] Taschler, U., Eichmann, T. O., Radner, F. P. W., Grabner, G. F., Wolinski, H., Storr, M., Lass, A., Schicho, R., and Zimmermann, R. Monoglyceride lipase deficiency causes desensitization of intestinal cannabinoid receptor type 1 and increased colonic μ-opioid receptor sensitivity Br J Pharmacol 2015, 172, 4419–4429. DOI: 10.1111/bph.13224

[4] Mayer, N., Schweiger, M., Romauch, M., Grabner, G. F., Eichmann, T. O., Fuchs, E., Ivkovic, J., Heier, C., Mrak, I., Lass, A., Hofler, G., Fledelius, C., Zechner, R., Zimmermann, R., and Breinbauer, R. Development of small-molecule inhibitors targeting adipose triglyceride lipase Nat Chem Biol 2013, 9, 785–787. DOI: 10.1038/nchembio.1359

[5] Taschler, U., Radner, F. P., Heier, C., Schreiber, R., Schweiger, M., Schoiswohl, G., Preiss-Landl, K., Jaeger, D., Reiter, B., Koefeler, H. C., Wojciechowski, J., Theussl, C., Penninger, J. M., Lass, A., Haemmerle, G., Zechner, R., and Zimmermann, R. Monoglyceride Lipase Deficiency in Mice Impairs Lipolysis and Attenuates Diet-induced Insulin Resistance J Biol Chem 2011, 286, 17467–17477. DOI: 10.1074/jbc.M110.215434

[6] Schweiger, M., Schoiswohl, G., Lass, A., Radner, F. P. W., Haemmerle, G., Malli, R., Graier, W., Cornaciu, I., Oberer, M., Salvayre, R., Fischer, J., Zechner, R., and Zimmermann, R. The C-terminal region of human adipose triglyceride lipase affects enzyme activity and lipid droplet binding J Biol Chem 2008, 283, 17211–1720. DOI: 10.1074/jbc.M710566200

[7] Schweiger, M., Schreiber, R., Haemmerle, G., Lass, A., Fledelius, C., Jacobsen, P., Tornqvist, H., Zechner, R., and Zimmermann, R. Adipose triglyceride lipase and hormone-sensitive lipase are the major enzymes in adipose tissue triacylglycerol catabolism J Biol Chem 2006, 281, 40236–40241. DOI: 10.1074/jbc.M608048200

[8] Haemmerle, G., Lass, A., Zimmermann, R., Gorkiewicz, G., Meyer, C., Rozman, J., Heldmaier, G., Maier, R., Theussl, C., Eder, S., Kratky, D., Wagner, E. F., Klingenspor, M., Hoefler, G., and Zechner, R. Defective lipolysis and altered energy metabolism in mice lacking adipose triglyceride lipase Science 2006, 312, 734–737. DOI: 10.1126/science.1123965

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[9] Lass, A., Zimmermann, R., Haemmerle, G., Riederer, M., Schoiswohl, G., Schweiger, M., Kienesberger, P., Strauss, J. G., Gorkiewicz, G., and Zechner, R. Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome Cell Metab 2006, 3, 309–319. DOI: 10.1016/j.cmet.2006.03.005

[10] Zimmermann, R., Strauss, J. G., Haemmerle, G., Schoiswohl, G., Birner-Gruenberger, R., Riederer, M., Lass, A., Neuberger, G., Eisenhaber, F., Hermetter, A., and Zechner, R. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase Science 2004, 306, 1383–1386. DOI: 10.1126/science.1100747

Patents

Zimmermann R, Zechner R, Haemmerle G, Hoefler G, Das S, Lass A. 2015. Method for

treatment of cachexia by administering inhibitors of adipose triglyceride lipase expression or

activity. WO2010115825A2

Hoefler G, Zechner R, Zimmermann R, Haemmerle G, Lass A, Das S. 2016. Modulating the

activity of triglyceride hydrolase. EP 1765997 B1

Schweiger M, Romauch M, Zimmermann R, Mayer N, Breinbauer R. 2014. Lipase inhibitors.

US 20140206738 A1/EP 2948433

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