Microscopy101
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Transcript of Microscopy101
![Page 1: Microscopy101](https://reader035.fdocuments.us/reader035/viewer/2022070316/555d0498d8b42a08668b577a/html5/thumbnails/1.jpg)
Microscopy 101
![Page 2: Microscopy101](https://reader035.fdocuments.us/reader035/viewer/2022070316/555d0498d8b42a08668b577a/html5/thumbnails/2.jpg)
Proper Handling
• Make sure cord is secure
• Support the base with one hand
• Lift with the arm of the scope
• Do not swing!
![Page 3: Microscopy101](https://reader035.fdocuments.us/reader035/viewer/2022070316/555d0498d8b42a08668b577a/html5/thumbnails/3.jpg)
Ocular
Arm
Stage
Base
Coarse Adjustment
Fine AdjustmentLight Source
Diaphragm
Stage Clips
Objectives
Nosepiece
Tube
![Page 4: Microscopy101](https://reader035.fdocuments.us/reader035/viewer/2022070316/555d0498d8b42a08668b577a/html5/thumbnails/4.jpg)
Magnification
Ocular = 10x
4x Objective 10x Objective 40x Objective
Therefore, 40x, 100x, 400x Magnification
![Page 5: Microscopy101](https://reader035.fdocuments.us/reader035/viewer/2022070316/555d0498d8b42a08668b577a/html5/thumbnails/5.jpg)
Getting Started
• Plug in & turn on
• Rotate nosepiece to place 4x objective into position
• Raise stage all the way up by turning the coarse adjustment knob
• Place specimen slide into place
• Look into ocular as you lower the stage VERY SLOWLY until low magnification image focuses
![Page 6: Microscopy101](https://reader035.fdocuments.us/reader035/viewer/2022070316/555d0498d8b42a08668b577a/html5/thumbnails/6.jpg)
Increasing Magnification & Focusing
• Once low magnification is focused, DO NOT touch coarse adjustment knob ever again!
• Switch to 10x objective by rotating nosepiece
• Use fine adjustment knob to focus
• Once focused, switch to 40x objective to view high magnification
• Focus with fine adjustment knob
![Page 7: Microscopy101](https://reader035.fdocuments.us/reader035/viewer/2022070316/555d0498d8b42a08668b577a/html5/thumbnails/7.jpg)
Before you leave…
• Return slides to proper boxes
• Turn off & unplug scopes
• Rotate to lowest objectives (4x)
• Raise stage all the way up with coarse adjustment knob
• Secure cords
• Return to cabinets, carrying properly