Lecture 6:

EVE 161:Microbial Phylogenomics

Lecture 6: Era II: Culture Independent rRNA

UC Davis, Winter 2016 Instructors: Jonathan Eisen & Holly Ganz

Where we are going and where we have been

• Previous lecture: !5. Modern view of Tree of Life

• Current Lecture: !6. Era II: rRNA from environment

• Next Lecture: !7: Era II: rRNA PCR

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• Problem set on archaea vs. eukaryote phylogeny papers will be posted today or emailed

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Paper #1

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Lane et al. Abstract

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Background: Why Sequences?

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Background: Why Sequences?

Background: Why rRNA

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Background: Why rRNA

Methods: How Get RNA

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Methods: How Make Primers

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Methods: Reverse Transcriptase

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Methods: Nucleotides

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Methods: Sequencing

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Results and Discussion

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Priming

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Priming

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Priming

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Errors

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Errors

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Alignment

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Phylogeny

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