micro research poster update11new
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Transcript of micro research poster update11new
“COMPARISON OF WATER QUALITY IN AND OUT OF STORMWATER POND” Kelynn Brengelman, Dr. David Buckalew
Longwood UniversityDepartment of Biological &Environmental Sciences
Purpose: To determine the quality of water that flows into and out of the stormwater pond on Longwood University’s campus, thus measuring the effectiveness of the retention of water in the pond.
Site 1: Adjacent to Edmunds St
2/2 2/9 2/23 3/1 3/15 3/21 3/29
104 8 468 48 256 3464 248
Site 2: Franklin St2/2 2/9 2/23 3/1 3/15 3/21 3/2
9
108 16 664 40 228 308 200
Site 3: Mouth of pond2/2 2/9 2/23 3/1 3/15 3/21 3/29
68 20 460 64 172 576 208
Site 4: Stormwater pond2/2 2/9 2/23 3/1 3/15 3/21 3/29
228 64 716 4 2596 2068 1952
Site 5: Downtown outlet behind church2/2 2/9 2/23 3/1 3/15 3/21 3/29
164 68 876 548 9680 2316 500
Site 6: Downtown outlet 22/2 2/9 2/23 3/1 3/15 3/21 3/29
200 56 692 336 9680 1740 568
Site 7: Downtown outlet 32/2 2/9 2/23 3/1 3/15 3/21 3/29
224 120 9680 228 220
Collection Protocol•Samples:- collected weekly at the same time of day- collected at least 48 hours after rain event- obtained from mid-column region facing upstream- collected by hand or dip method
Laboratory Protocol• Add Colilert® medium to 25mL sample 75ml sterile water mixture• Add mixture to Quanti-Tray® and seal• Incubate for 24 +/- 2 hours at 44.2°C• Bacterial numbers were obtained using the IDEXX Quanti-Tray MPN table
PURPOSE
METHODOLOGIES
BACKGROUND• E. coli are used as indicator species which may signal the presence of potential human pathogens including: Cryptosporidium, Giardia, Shigella, Salmonella and norovirus. • Fecal contamination can lead to gastrointestinal illness, skin, eye, neurologic, and wound infections.
RESULTS
GIS MAP OF SAMPLE SITES CONCLUSIONS Regular maintenance and upkeep is necessary to maintain the integrity of the pond. - Bacterial numbers increase within
the pond and downstream. - Pond is not currently serving its
purpose.- Presence of ducks may contribute
to high E.Coli count.- Replant select wetland species to
improve water quality.- Output drain from pond may need
to be lowered- Changes could improve water
qualities in Appomattox river, James river, Chesapeake bay.
- Poses public health implications regarding drinking water.
INITIAL VS CURRENT STATE OF POND
INITIAL CURRENT
ACKNOWLEDGEMENTS
REFERENCESLewis, Lori. “Health Implications of Escherichia coli (e.coli) in Recreational and Drinking Water.” The Water Project. N.d. Web. 19 April, 2016.“Stormwater Wet Pond and Wetland Management Guidebook.” United States Environmental Protection Agency.” 2009. Web. 19 April, 2016.Clary, Jane et. al “Can Stormwater BMPs Remove Bacteria?” Wa Stormwater Center. 2007. Web. 19 April, 2016.
Thank you to Dr. Buckalew for his extensive help and guidance throughout this project, and thank you to the Longwood Department of Biological & Environmental Sciences.Finally, many thanks to Jessica Hoak for assistance with the GIS map.
1 2 3 4 5 6 70
500
1000
1500
2000
2500
Average E.coli Count
Average E.coli by Site
# E.
coli
per 1
00m
L
1 2 3 4 5 6 70
200
400
600
800
1000
1200
1400
1600
1800
2000
MPN Numbers by Date & Site
2-Feb 9-Feb 23-Feb 1-Mar 29-Mar AvgSite Number
Mos
t Pro
babl
e N
umbe
rs (M
PN)