Mesa 2.4. myriam calle

Mesa 2. Control y biomarcadores

Dra. Myriam

Calle Rubio

Hospital Clínico Universitario

San Carlos. Madrid


[ATS] Inflammatory

Markers In Different COPD

Subgroups Compared To

Smokers

And Healthy Controls

Behndig AF


Curtis et al: Proc Am Thorac Soc 2007; 4:512-521


Inflammatory Markers in Different COPD Subgroups

Compared to Smokers and Healthy Controls

The chronic airway inflammatory response in COPD is suggested to be a result of both

innate and adaptive immune responses.

Natural killer cells (NK) and CD56-expressing T cells are cytotoxic lymphocytes that have

been implicated in COPD pathogenesis. Natural Killer cell group 2 D (NKG2D) receptors

have also been shown to be involved in emphysema development.Titular tabla

We hypothesised that cytotoxic T cells would be associated to the rate of lung function

decline in subjects with COPD. Two groups of COPD subjects were recruited, rapid

decliners (loss of FEV1 of > 60 ml/year) and slow decliners (loss of FEV1 of < 25 ml/year).

Am J RespirCrit Care Med 191;2015:A2884


METHODS

As controls, we included two groups; smokers with normal lung function and healthy never

smoking individuals. All subjects were recruited from the OLIN-cohort (Obstructive Lung

disease In Northern Sweden) that has been followed with spirometry annually for a period

of more than 10 years.

Bronchoscopy with bronchoalveolar lavage was performed. T cell subsets were

determined in BAL-fluid from 17 individuals with COPD stage II-III (12 rapid decliner

and 5 slow decliners), and compared to data from 15 smokers with normal lung

function and 14 healthy never-smokers. The cells were stained with monoclonal

antibodies against CD3, CD8, CD16, CD56 and NKG2D, and analyzed using flow

cytometry.

Am J RespirCrit Care Med 191;2015:A2884


RESULTS

In BAL-fluid NK-cells (CD3-/CD16+/CD56+) were higher in COPD subjects compared to healthy

(p=0.013). The expression of CD 16+/CD56+ on CD3+lymphocytes was increased both in

subjects with COPD with rapid decline in lung function (p<0.001), in COPD with slow decline

(p=0.0082) as well as in smokers with normal lung function (p=0.0065), all compared to healthy

non-smokers. Furthermore, the activating receptor NKG2D on CD8 positive lymphocytes was

found to be up-regulated in rapid decliners (p=0.0078) compared to healthy, but not in slow

decliners and in smokers.

CONCLUSIONS

NK-cells and CD56-expressing T cells were increased in COPD subjects regardless of rapid

or slow lung function decline. These data do not support the hypothesis that cytotoxic T cells can

be predictive for the rate of lung function decline in COPD. The increase in NKG2D in rapid

decliners may indicate that these cells can play a role in lung function decline. Further

research is warranted to confirm this finding.


[SEPAR] Evaluación de

redes de interacción entre

biomarcadores sistémicos

de reactantes de fase aguda

en la EPOC

Calerio C


EPOC e inflamación


¿Sus implicaciones?

• Se desconocen sus mecanismos

de génesis.

• Se relaciona con comorbilidades,

algunos resultados clínicos y el

pronóstico.


¿Papel de los marcadores de inflamación en el suero de los

pacientes con EPOC?Estudio Ciudad de Copenhague

N= 1302 EPOC

Seguimiento 8 años

Dahl et al; AJRCCM 2007; 175:250-255


Subtítulo de la diapositiva

•Cabecera párrafo

Titular tabla

El objetivo era evaluar la relación entre diversos biomarcadores que actúan como

reactantes de fase aguda en la EPOC, para aproximarnos a conocer su impacto e

implicaciones.


Estudio observacional, trasversal de pacientes con EPOC en fase

estable.

Determinación de 9 proteínas: alfa 2 macroglobulina, haptoglobina, PCR,

amiloide sérico P, procalcitonina, ferritina, activador tisular del

plasminógeno (tPA), fibrinógeno, amiloide sérico A.

Se estudiaron las correlaciones

bivariadas mediante un mapa de

calor.


Existe un patrón de relaciones entre los biomarcadores reactantes de

fase aguda en la EPOC, que se modifica respecto a fumadores sanos y

distintos grados de afectación funcional.


Muchas gracias

por su atención

Mesa 2.4. myriam calle

Health & Medicine

Transcript of Mesa 2.4. myriam calle