Methods in Enzymology

Volume 158

MetallobiochemistryPart A

James F. Riordan

Bert L . Vallee

CONTRIBUTORS TO VOLUME 158 vi i

PREFACE i X

VOLUMES IN SERIES X i

Section I . Sample Preparatio n

1. Preparation of Metal-Free Water

JAMES F . RIORDAN AN D

BERT L . VALLEE

3

2. Elimination of Adventitious Metals

BARTON HOLMQUIST

6

3. Metal-Free Dialysis Tubing

DAVID S . AULD

1 3

4. Metal-Free Chromatographic Media

MARK T . MARTIN

1 5

5. Preparation of Metal-Free Enzymes

FRED W . WAGNER

2 1

6. Metal-Buffered Systems

JOHN O . BAKER

3 3

7. Standards for Metal Analysis

CLAUDE VEILLON

5 6

8. Methods for Metal Substitution

DAVID S . AULD

7 1

9. Preparation of Metal-Hybrid Enzymes

WOLFGANG MARET AND

MICHAEL ZEPPEZAUER

79

10 . Introduction of Exchange-Inert Metal Ions into HAROLD E . VAN WART

95

Enzymes

11. Use of Chelating Agents to Inhibit Enzymes

DAVID S . AULD

11 0

Section II . Analytical Techniques

12. Atomic Absorption Spectrometry

WALTER SLAVIN

11 7

13 . Multielement Atomic Absorption Methods of JAMES M . HARNLEY AN D

Analysis

DONITA L . GARLAND

145

14. Ion Microscopy in Biology and Medicine

SUBHASH CHANDRA AN D

GEORGE H . MORRISON

157

15. Flame Atomic Emission Spectrometry

TERENCE H . RISBY

180

16 . Inductively Coupled Plasma-Emission Spectrome- KAREN A . WOLNIK

190

try

17 . Inductively Coupled Plasma-Mass Spectrometry Jost A . OLIVARES

20 5

18 . Atomic Fluorescence Spectrometry

ROBERT G . MICHEL

22 2

19 . Electrochemical Methods of Analysis

JANET OSTERYOUNG

24 3

20 . Neutron Activation Analysis

JACQUES VERSIECK

267

Section III . Analysis of Metals

21 . Aluminum

JOHN SAVORY, SUE BROWN ,

ROGER L . BERTHOLF ,

NANCY MENDOZA, AND

MICHAEL R . WILLS

28 9

22 . Measurement of Total Calcium in Biological Flu- GEORGE N . BOWERS, JR .ids : Flame Atomic Absorption Spectrometry

AND THEODORE C . RAINS

30 2

23 . Measurement of Ionized Calcium in Biological SALVADOR F . SENA AND

Fluids : Ion-Selective Electrode Method

GEORGE N. BOWERS, JR .

32 0

24 . Chromium

CLAUDE VEILLON

334

25 . Determination of Cobalt by Atomic Absorption ROBERT SHAPIRO AN D

Spectrometry

MARK T . MARTIN

344

26 . Measurement of Copper in Biological Samples by MERLE A . EVENSON

35 1Flame or Electrothermal Atomic AbsorptionSpectrometry

27 . Rapid Colorimetric Micromethod for the Quantita- WAYNE W. FISH

357tion of Complexed Iron in Biological Samples

28 . Atomic Absorption Spectrometry of Magnesium MARK T . MARTIN AN D

ROBERT SHAPIRO

365

29 . Molybdenum

JEAN L . JOHNSON

37 1

30 . Nickel Analysis by Electrothermal Atomic Ab- F . WILLIAM SUNDERMAN, JR . ,sorption Spectrometry

SIDNEY M . HOPFER, AND

M . CRISTINA CRISOSTOMO

38 2

31 . Determination of Selenium in Biological Matrices S . A . LEWIS

39 1

32 . Vanadium

DONNA M . MARTIN AN D

N . DENNIS CHASTEEN

40 2

33 . Determination of Zinc in Biological Samples by K. H . FALCHUK, K . L . HILT,Atomic Absorption Spectrometry

AND B . L . VALLEE

42 2

AUTHOR INDEX 43 5

SUBJECT INDEX 451