March 3, 2010 Introduction to development Gene expression.
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Transcript of March 3, 2010 Introduction to development Gene expression.
What cellular functions are needed to carry out development?
DivideGrowDifferentiateDieMoveAdhereSecreteSignal
Cell biological properties need to be coordinated in space and time
Why do different cells behave differently?
Have different genes?Have different histories?Experience different environments?Chance?Have different gene expression states?
Why do different cells behave differently?
Have different genes?Have different histories?Experience different environments?Chance?Have different gene expression states?
08_03_control.steps.jpg
mRNA localization control(tether mRNA to localized proteins)
mRNA turnover control(microRNAs)
Protein turnover control(ubiquitylation and proteasome digestion)
Protein localization control
Regulation of gene expression
08_03_control.steps.jpg
mRNA localization control(tether mRNA to localized proteins)
mRNA turnover control(microRNAs)
Protein turnover control(ubiquitylation and proteasome digestion)
Protein localization control
Regulation of gene expression
05_30_histone tails.jpgHistone modifications affect gene expression
Can affect recruitment of transcription factors to promoter
From Wildwater et al., Cell 123: 1337-1349 (2005)
Rb-Related expression in Arabidopsis embryosby in situ hybridization
PARF6::ARF6::GUS fusion expression in flowers and ovules
Promoter:protein:reporter fusion gene – reveals protein location
Kosman et al., Science 254: 118-122 (1991)
anti-Snailanti-Twist
Immunolocalization of Snail and Twist proteins in Drosophila embryos