MarbankMarbank-a marine biobank a marine biobank locatedlocated in Tromsø, Norway in Tromsø, Norway

Kjersti Lie Gabrielsen

Main objectives MarbankMain objectives Marbank

The mission of Marbank is to The mission of Marbank is to provide an accessible national provide an accessible national repository of frozen marine repository of frozen marine biological samples, collected and biological samples, collected and maintained under rigorously maintained under rigorously controlled conditionscontrolled conditions

Marbank CollectionMarbank Collection

The material to be archived and The material to be archived and stored in the Marbank repository stored in the Marbank repository will include genetic and biological will include genetic and biological material from marine material from marine microorganisms, plankton, algae, microorganisms, plankton, algae, invertebrates and vertebrates invertebrates and vertebrates

Marbank Marbank samplessamples

Organism/tissue

Water extract

Organic extract

DNA/ RNA (cDNA)

Storage

Photos: Klara Stensvåg, NFH

Marbank StorageMarbank Storage

- LLNN22-tanks and freezers -tanks and freezers

with temperature control/ with temperature control/ alarmalarm systems systems

Uniform and high quality storage of Uniform and high quality storage of samples at low temperaturessamples at low temperatures

Marbank CustomersMarbank Customers

Marbank will offer services for Marbank will offer services for researchers, legislation and industryresearchers, legislation and industry

Access to samples and data information Access to samples and data information from Marbank will be regulated by a set from Marbank will be regulated by a set of policiesof policies

R&D institutions and industry will gain R&D institutions and industry will gain access to the material after description access to the material after description of scientific purposesof scientific purposes

Database MarbankCollection

Administrative data:

-Policies-Loan/transactions

Taxonomy/physical characteristics:

-Specie-Name/Common name-Determined by-Weight-Length-Sex/age-Reference literature

Field data:

-Date-GIS/location-Field prep-Field notes-Hazard-Storage-Voucher

Storage:

-Tissue/extract-Preservation-Initial weight-Current weight-# individuals -Freezer name etc.-Position in archive (section, rack, box)

Digital images:

-Photo-Video

Literature:-Articles etc.

External Server MarbioCustomersWeb-site

Marbank strategies for collection of Marbank strategies for collection of marine samplesmarine samples

Initiation of dedicated Marbank surveys/ Initiation of dedicated Marbank surveys/ hiring of research vessels hiring of research vessels

Marbank participation on surveys Marbank participation on surveys initiated by other institutionsinitiated by other institutions

Receipt of living or frozen samples Receipt of living or frozen samples collected by researchers from other collected by researchers from other institutionsinstitutions

Marbank Marbank initiators/initiators/collaboratorscollaborators

University of Tromsø University of Tromsø Institute of Marine ResearchInstitute of Marine Research Norwegian Polar InstituteNorwegian Polar Institute Norwegian Institute of Fisheries and Norwegian Institute of Fisheries and

Aquaculture ResearchAquaculture Research

Collaboration Marbank - Collaboration Marbank - MarbioMarbio

CollectionCollection ExtractionExtraction

Bioactivity Bioactivity screeningscreening

FractionationFractionationPurificationPurification

MarbankMarbank

MarbioMarbio

DatabaseDatabase

Marbio: Medium/high throughput Marbio: Medium/high throughput screening of novel bioactivitiesscreening of novel bioactivities

Biomek NX S8 W/ gripper

1 meter ORCA

CO2

incubator

ShakerPlate reader

Plate washer

Platehotel BC

Anti-tumorAnti-tumor Anti-bacterial Anti-bacterial Anti-viralAnti-viral Anti-inflammatory Anti-inflammatory Immunostimulatory Immunostimulatory Enzymes

SummarySummary Marbank will build and maintain a Marbank will build and maintain a

collection of marine samplescollection of marine samples

The frozen tissue samples and extracts will The frozen tissue samples and extracts will represent the genetic material of a wide represent the genetic material of a wide range of species range of species

The collection will be an archive of The collection will be an archive of molecular biodiversity and will make molecular biodiversity and will make unique and important research materials unique and important research materials available to the scientific communityavailable to the scientific community