Magnetic Sector GC-HRMS with DualData XL Option: Helium … · 2019-08-15 · Magnetic Sector...
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Thermo Fisher Scientific • Hanna-Kunath Str. 11 • Bremen, 28199, Germany • thermofisher.com ABSTRACT Purpose: Helium shortage is a global concern affecting operational costs of GC Analysis. Another important economic factor for a laboratory is the analysis time. Added to the intrinsic high sensitivity and robustness - due to the large ion source - for dioxin and POPs analysis of a Magnetic Sector High Resolution Mass Spectrometer the attachment of 2 GCs to one single MS strongly increases its flexibility. The DualData XL Option of the Thermo Scientific™ DFS™ Magnetic Sector GC-HRMS is a technical implementation based on a double GC configuration which allows at increasing the sample throughput. Effectively the productivity in number of samples injected on one single MS is almost doubled. In addition to the time-saving, by using this option combined with an Instant Connect Helium Saver Injector Module and a flow optimized DualData XL method, helium consumption can be drastically reduced. CONCLUSIONS It has been demonstrated that the DualData XL Option allows a higher sample throughput by no loss in performance such as peak shape or sensitivity. Increase of productivity up to double sample throughput. Excellent peak shape using MCD wafer technology. No loss in sensitivity compared to a standard dual GC System. Drastic reduction of helium consumption by using the Instant Connect Helium Saver Injector. Applicable to different POPs such as dioxins, PCBs and PBDEs. Statement of the findings, indicating future research directions. REFERENCES 1.U.S. Environmental Protection Agency, Method EPA 1613 Rev. B, Washington, October 1994. 2. European Committee for Standardization, EN 1948, Brussels, December 1996. 3. Application Note AN30098, Thermo Fisher Scientific, Bremen 2006. TRADEMARKS/LICENSING © 2019 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries. This information is not intended to encourage use of these products in any manner that might infringe the intellectual property rights of others. PO30642 Figure 1. Thermo Scientific DFS Magnetic Sector GC-HRMS coupled to two Thermo Scientific TRACE 1310 GC. Figure 5. Principle of the DFS GC-HRMS Dual Data Acquisition column switching system for dynamic flow switching of the GC effluent and inner view of a micro fluidic channel device (MCD) wafer. Each GC was equipped with a dynamic flow switching system which allows at dividing the GC eluate to waste or to the MS source (see Figure 4). The ability to cut out parts of the chromatogram in order to prevent any cross contamination caused by the second GC run was checked by a measurement of EPA 1613 CS5 standard. Furthermore the ability to cut out the solvent was proved by monitoring the MS Ion source pressure while injection solvent up to a volume of 10ul into the system. The solvent don’t reach the source, when the valves are in ON position. In the meantime the flow into the ion source will be compensated by helium delivered by a computer controllable carrier gas module in order to keep the pressure in the ion source constant. The helium makeup gas in can be reduced on both GCs in a way that the function of the dynamic flow switching system is given but reduce the ion source pressure during an alternating Sequence and saves helium at the same time. Heinz Mehlmann, Dirk Krumwiede, Thermo Fisher Scientific, Thermo Fisher Scientific, Hanna-Kunath Str. 11, Bremen, Germany, 20199 Magnetic Sector GC-HRMS with DualData XL Option: helium savings and flow optimization for maximized productivity and cost savings for PBDE, dioxin and PCB analysis In a typical experiment, the first GC run was started, and during the wait time of 20 minutes, while the solvent peak as well as other compounds of no interest eluted all GC eluate was diverted to waste. After 20 minutes, the GC eluate was directed to the ion source of the MS and MS data acquisition started. At approximately the same time, a second sample was injected to the second GC, running the same process as the first one. (i.e. during the first 20 minutes no GC eluate was directed towards the MS. Once the first GC finished cooling down the oven to start condition, another injection occurred, and the same scheme as denoted above repeats). This resulted in two GCs running simultaneously with staggered sample injections. Only the retention time windows of interest from each GC were directed to the MS for data acquisition. METHODS The configuration used in this study consists of two Thermo Scientific™ TRACE 1310™ GC equipped with the DualData XL Option using two columns coupled to the DFS Magnetic Sector GC-HRMS. The mass spectrometer was set up in a multiple ion detection mode (MID) at a resolution of 10,000 (10% valley definition). FC43 and PFK was used as a reference compound to provide the inherent lock and cali masses. The Thermo Scientific™ TriPlus RSH Autosampler with extended x-rail served both GCs from one common sample tray. Typically one μL of sample was injected in split less mode using a Thermo Scientific Instant Connect Helium Saver Injector Module. A method 1613 CS1 – CS5 calibration standard (1:10 diluted from Cambridge Isotope Laboratories) and CS3 / CPM 8290/1613 was used as well as method 1668 and 1614 standards to demonstrate the chromatographic performance of the system. It was found that the effects due to some increased dead volumes and a disturbed flow path caused by the MCD were negligible. No additional peak tailing was observed and the requirements of EPA 1613 to separate the 2,3,7,8 TCDD to the next eluting TCDD with a valley of better than 25% was easy achievable . Also other POPs such as PBDEs or PCBs can be run with the Dual Data XL Option as well as combinations of different applications per GC on one Dual Data XL System. e.g. dioxins on GC1 60m column and PBDEs on GC2 using a 15m column. Due to the typically acquisition time of those applications the PBDEs can be acquired while the dioxins are already injected but still in the waiting time frame. In such a sequence for each dioxin sample, an additional PBDE run can be acquired in the same time compared to a standard sequence only for dioxins on a single GCMS System. Methods: For all gas chromatographic analyses a certain amount of ‘dead’ time is an intrinsic part of the measurement. The dead time is the time before the first relevant peak is detected and after the last relevant peak elutes. Dioxin analyses are typically conducted using 60 m columns that result in run times of 50-60 minutes. The dead time for such analyses can be up to 20-30 minutes per sample. The dead time can be almost eliminated by performing alternate staggered injections using two GCs coupled to one MS. First, both GCs are running in parallel. Secondly, the timing is such that the chromatogram section with peaks from one GC falls into the chromatogram section with no peaks from the other GC and vice versa. To realize a time- controlled staggered injection sample sequence specific software is needed and some hardware modifications inside of each GC need to be implemented. These modifications ensure that at any point of time the flow of one GC only – time section with peaks - is guided into the mass spectrometer for measurement while the flow of the other GC – time section without peaks - is directed into waste. A time controlled dynamic flow switching system using a proprietary micro fluidic channel device (MCD) allows to switch flow between vacuum purge and MS (see Figure 5). Results: Chromatograms with and without the MCD device are practically undistinguishable from one another in terms of peak shape or sensitivity. The concept was proven by numerous experiments and is by now validated in full production dioxin analysis laboratories. The Thermo Scientific DualData Option for the DFS Magnetic Sector GC-HRMS can be used for a range of POPs analyses applications such as dioxins, PCBs or PBDEs. Also a combination of different applications is possible. With an increase of more than 90% the sample throughput for e.g. dioxins is almost doubled. Using the Instant Connected Helium Saver Injector Module in combination with the DualData option is an efficient way to save analysis time and Helium. INTRODUCTION The sequence timeline on a standard dual GC configuration is interrupted by segments of dead time (see Figure 3a). In the sequence timeline of a Dual Data equipped dual GC configuration a staggered injection is possible with no dead time between each data acquisition (see Figure 3b). Figure 2. Timescale of a typical dioxin GC. Figure 3a. Normal dual GC configuration: The gray area represents dead time, where no data is acquired. Figure 3b. DualData Acquisition sequence. Only the relevant retention time windows of both GCs are monitored. Valves Injector Vacuum He Carrier Gas Module Purge MCD wafer Column to DFS Capillaries GC oven GC Module Ø 25mm Vacuum Column in He Column out Figure 6. Flow of the analytical column of GC 1 is guided into the MS and is acquired while the flow of GC2 is directed into waste (upper schematic) and vice versa (lower schematic). RT: 38.44 - 43.53 38.5 39.0 39.5 40.0 40.5 41.0 41.5 42.0 42.5 43.0 43.5 Time (min) 0 50 100 0 50 100 0 50 100 0 50 100 0 50 100 Relative Abundance 0 50 100 0 50 100 0 50 100 39.39 41.88 41.16 41.01 42.91 41.88 41.16 39.39 41.01 42.89 41.86 41.14 40.99 42.89 41.86 41.14 40.99 42.88 40.16 42.51 42.19 40.16 42.19 42.51 42.08 42.18 42.49 42.06 42.18 42.49 42.06 NL: 5.27E5 NL: 4.28E5 NL: 5.76E5 NL: 1.07E6 NL: 3.87E5 NL: 3.06E5 NL: 7.43E5 NL: 5.95E5 Figure 8. Example of peak integrity of dioxin trace analysis (Hexa CDD/F) using the DualData Acquisition. native HxCDF (373.8202 m/z) native HxCDF (375.8172 m/z) 13 C 12 Standard HxCDF (383.8633 m/z) 13 C 12 Standard HxCDF (385.8604 m/z) native HxCDD (389.8151 m/z) native HxCDD (391.8121 m/z) 13 C 12 Standard HxCDD (401.8554 m/z) 13 C 12 Standard HxCDD (403.8524 m/z) Column: Thermo TR-Dioxin 60m x 0.25mm ID x 0.1µm film; Oven: 150 (2)-25-230(25)-6-320(6) 2,3,7,8-TCDD RT: 22.57 - 31.20 23 24 25 26 27 28 29 30 31 Time (min) 20 40 60 80 100 20 40 60 80 100 20 40 60 80 100 Relative Abundance 20 40 60 80 100 23.07 30.69 28.71 28.42 28.12 27.52 23.07 30.69 28.71 28.42 28.11 27.52 28.66 27.49 28.66 27.49 NL: 3.20E4 NL: 4.28E4 NL: 2.51E5 NL: 3.21E5 native TCDD (319.8960 m/z) native TCDD (321.8930 m/z) 13 C 12 Standard TCDD (331.9363 m/z) 13 C 12 Standard TCDD (333.9333 m/z) < 10% Column: Thermo TR-Dioxin 60m x 0.25mm ID x 0.1µm film; Oven: 150 (2)-25-230(25)-6-320(6) Figure 9. Separation of the 2,3,7,8-TCDD to the next eluting TCDD. The valley is below 25% as required by EPA1613. RT: 5.67 - 26.50 6 8 10 12 14 16 18 20 22 24 26 Time (min) 0 50 100 0 50 100 0 50 100 0 50 100 Relative Abundance 0 50 100 0 50 100 0 50 100 8.16 8.36 7.88 7.25 10.83 11.25 10.31 9.66 13.61 12.40 14.37 15.64 15.77 17.24 15.63 15.93 16.49 17.22 20.11 17.96 19.73 20.23 21.79 18.08 21.78 18.89 21.99 19.00 22.44 17.81 20.11 25.50 NL: 7.60E5 m/z= 327.8815- 327.9015 MS D90311A20 NL: 4.34E5 m/z= 405.7981- 405.8181 MS D90311A20 NL: 3.09E5 m/z= 485.7010- 485.7210 MS D90311A20 NL: 3.47E5 m/z= 563.6120- 563.6320 MS D90311A20 NL: 3.93E5 m/z= 643.5200- 643.5400 MS D90311A20 NL: 1.97E5 m/z= 721.4310- 721.4510 MS D90311A20 NL: 1.97E4 m/z= 799.3230- 799.3430 MS D90311A20 RT: 16.39 - 23.75 17 18 19 20 21 22 23 Time (min) 0 20 40 60 80 100 Relative Abundance 0 20 40 60 80 100 Relative Abundance 0 20 40 60 80 100 Relative Abundance 0 20 40 60 80 100 Relative Abundance RT: 20.27 AA: 439636 RT: 17.14 AA: 181218 RT: 21.67 AA: 202188 RT: 20.08 AA: 206028 RT: 20.85 AA: 199819 RT: 23.54 AA: 152336 RT: 20.27 AA: 276265 RT: 20.08 AA: 131558 RT: 17.14 AA: 115798 RT: 21.67 AA: 127874 RT: 20.85 AA: 121786 RT: 23.54 AA: 102118 RT: 18.28 AA: 197852 RT: 20.25 AA: 202457 RT: 17.12 AA: 164137 RT: 21.64 AA: 182319 RT: 23.52 AA: 161317 RT: 18.28 AA: 124122 RT: 20.25 AA: 125141 RT: 17.12 AA: 103963 RT: 21.66 AA: 113758 RT: 23.52 AA: 100446 NL: 1.37E5 m/z= 325.6574- 326.0329 MS ICIS X4-210313- 1S3 NL: 8.59E4 m/z= 327.7226- 328.0981 MS ICIS X4-210313- 1S3 NL: 6.17E4 m/z= 337.7672- 338.1427 MS ICIS X4-210313- 1S3 NL: 3.98E4 m/z= 339.6447- 340.2079 MS ICIS X4-210313- 1S3 Figure 10a. Example of PCB trace analysis (5Cl PCB) Figure 10b. Example of PBDEs trace analysis. DFS OFF OFF ON ON GC1 GC 2 OFF OFF ON ON DFS GC1 GC 2 Figure 4. TRACE 1310 GC with DualData XL module (left front slot), standard SSL Injector (right front slot) and the Instant Connect Helium Saver Injector Module (back slots) mounted on the DFS Magnetic Sector GC-HRMS. RESULTS The analytical performance with DualData XL Acquisition and conventional GC/MS configuration was compared using the same set of polychlorinated dioxins and furans, PCBs and PBDE samples as model compounds. Sensitivity was compared by using low concentrated PCDD/PCDF standards as well as a low level pooled blood sample. The GC separation integrity, ruggedness and long-term stability of the column switching system have been proven in unattended sample. The Instant Connect Helium Saver Injector Module can be used on the DFS Magnetic Sector GC-HRMS in combination with the DualData XL Option. The Instant Connect Helium Saver Injector Module is an innovative split/splitless (SSL) inlet system capable of dramatically reducing helium consumption. Figure 7. Schematic flow diagram of the Instant Connect Helium Saver Injector Module during Injection (left side) and during data acquisition. The septum purge and the split flow is substitute by nitrogen. During the injection, a small portion of nitrogen enters the analytical column but such as the solvent it will be guided into the service vacuum by the DualData XL flow switching system. Figure 7. The module and injector components of the Instant Connect Helium Saver Injector Module.
Transcript of Magnetic Sector GC-HRMS with DualData XL Option: Helium … · 2019-08-15 · Magnetic Sector...
Thermo Fisher Scientific • Hanna-Kunath Str. 11 • Bremen, 28199, Germany • thermofisher.com
ABSTRACT Purpose: Helium shortage is a global concern affecting operational costs of GC Analysis. Another important economic factor for a laboratory is the analysis time. Added to the intrinsic high sensitivity and robustness - due to the large ion source - for dioxin and POPs analysis of a Magnetic Sector High Resolution Mass Spectrometer the attachment of 2 GCs to one single MS strongly increases its flexibility. The DualData XL Option of the Thermo Scientific™ DFS™ Magnetic Sector GC-HRMS is a technical implementation based on a double GC configuration which allows at increasing the sample throughput. Effectively the productivity in number of samples injected on one single MS is almost doubled. In addition to the time-saving, by using this option combined with an Instant Connect Helium Saver Injector Module and a flow optimized DualData XL method, helium consumption can be drastically reduced.
CONCLUSIONSIt has been demonstrated that the DualData XL Option allows a higher sample throughput by no loss in performance such as peak shape or sensitivity.
Increase of productivity up to double sample throughput.
Excellent peak shape using MCD wafer technology.
No loss in sensitivity compared to a standard dual GC System.
Drastic reduction of helium consumption by using the Instant Connect Helium Saver Injector.
Applicable to different POPs such as dioxins, PCBs and PBDEs. Statement of the findings, indicating future research directions.
REFERENCES 1. U.S. Environmental Protection Agency, Method EPA 1613 Rev. B, Washington,
October 1994.
2. European Committee for Standardization, EN 1948, Brussels, December 1996.
3. Application Note AN30098, Thermo Fisher Scientific, Bremen 2006.
TRADEMARKS/LICENSING© 2019 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries. This information is not intended to encourage use of these products in any manner that might infringe the intellectual property rights of others.
PO30642
Figure 1. Thermo Scientific DFS Magnetic Sector GC-HRMS coupled to two Thermo Scientific TRACE 1310 GC.
Figure 5. Principle of the DFS GC-HRMS Dual Data Acquisition column switching system for dynamic flow switching of the GC effluent and inner view of a micro fluidic channel device (MCD) wafer.
Each GC was equipped with a dynamic flow switching system which allows at dividing the GC eluate to waste or to the MS source (see Figure 4). The ability to cut out parts of the chromatogram in order to prevent any cross contamination caused by the second GC run was checked by a measurement of EPA 1613 CS5 standard. Furthermore the ability to cut out the solvent was proved by monitoring the MS Ion source pressure while injection solvent up to a volume of 10ul into the system. The solvent don’t reach the source, when the valves are in ON position. In the meantime the flow into the ion source will be compensated by helium delivered by a computer controllable carrier gas module in order to keep the pressure in the ion source constant. The helium makeup gas in can be reduced on both GCs in a way that the function of the dynamic flow switching system is given but reduce the ion source pressure during an alternating Sequence and saves helium at the same time.
Heinz Mehlmann, Dirk Krumwiede, Thermo Fisher Scientific, Thermo Fisher Scientific, Hanna-Kunath Str. 11, Bremen, Germany, 20199
Magnetic Sector GC-HRMS with DualData XL Option: helium savings and flow optimization for maximized productivity and cost savings for PBDE, dioxin and PCB analysis
In a typical experiment, the first GC run was started, and during the wait time of 20 minutes, while the solvent peak as well as other compounds of no interest eluted all GC eluate was diverted to waste. After 20 minutes, the GC eluate was directed to the ion source of the MS and MS data acquisition started. At approximately the same time, a second sample was injected to the second GC, running the same process as the first one. (i.e. during the first 20 minutes no GC eluate was directed towards the MS. Once the first GC finished cooling down the oven to start condition, another injection occurred, and the same scheme as denoted above repeats). This resulted in two GCs running simultaneously with staggered sample injections. Only the retention time windows of interest from each GC were directed to the MS for data acquisition.
METHODSThe configuration used in this study consists of two Thermo Scientific™ TRACE 1310™ GC equipped with the DualData XL Option using two columns coupled to the DFS Magnetic Sector GC-HRMS. The mass spectrometer was set up in a multiple ion detection mode (MID) at a resolution of 10,000 (10% valley definition). FC43 and PFKwas used as a reference compound to provide the inherent lock and cali masses. The Thermo Scientific™ TriPlus RSH Autosampler with extended x-rail served both GCs from one common sample tray. Typically one μL of sample was injected in split less mode using a Thermo Scientific Instant Connect Helium Saver Injector Module. A method 1613 CS1 – CS5 calibration standard (1:10 diluted from Cambridge Isotope Laboratories) and CS3 / CPM 8290/1613 was used as well as method 1668 and 1614 standards to demonstrate the chromatographic performance of the system.
It was found that the effects due to some increased dead volumes and a disturbed flow path caused by the MCD were negligible. No additional peak tailing was observed and the requirements of EPA 1613 to separate the 2,3,7,8 TCDD to the next eluting TCDDwith a valley of better than 25% was easy achievable .
Also other POPs such as PBDEs or PCBs can be run with the Dual Data XL Option as well as combinations of different applications per GC on one Dual Data XL System. e.g. dioxins on GC1 60m column and PBDEs on GC2 using a 15m column. Due to the typically acquisition time of those applications the PBDEs can be acquired while the dioxins are already injected but still in the waiting time frame. In such a sequence for each dioxin sample, an additional PBDE run can be acquired in the same time compared to a standard sequence only for dioxins on a single GCMS System.
Methods: For all gas chromatographic analyses a certain amount of ‘dead’ time is an intrinsic part of the measurement. The dead time is the time before the first relevant peak is detected and after the last relevant peak elutes. Dioxin analyses are typically conducted using 60 m columns that result in run times of 50-60 minutes. The dead time for such analyses can be up to 20-30 minutes per sample.
The dead time can be almost eliminated by performing alternate staggered injections using two GCs coupled to one MS. First, both GCs are running in parallel. Secondly, the timing is such that the chromatogram section with peaks from one GC falls into the chromatogram section with no peaks from the other GC and vice versa. To realize a time-controlled staggered injection sample sequence specific software is needed and some hardware modifications inside of each GC need to be implemented. These modifications ensure that at any point of time the flow of one GC only – time section with peaks - is guided into the mass spectrometer for measurement while the flow of the other GC – time section without peaks - is directed into waste. A time controlled dynamic flow switching system using a proprietary micro fluidic channel device (MCD) allows to switch flow between vacuum purge and MS (see Figure 5).
Results: Chromatograms with and without the MCD device are practically undistinguishable from one another in terms of peak shape or sensitivity. The concept was proven by numerous experiments and is by now validated in full production dioxin analysis laboratories. The Thermo Scientific DualData Option for the DFS Magnetic Sector GC-HRMS can be used for a range of POPs analyses applications such as dioxins, PCBs or PBDEs. Also a combination of different applications is possible. With an increase of more than 90% the sample throughput for e.g. dioxins is almost doubled. Using the Instant Connected Helium Saver Injector Module in combination with the DualData option is an efficient way to save analysis time and Helium.
INTRODUCTIONThe sequence timeline on a standard dual GC configuration is interrupted by segments of dead time (see Figure 3a). In the sequence timeline of a Dual Data equipped dual GC configuration a staggered injection is possible with no dead time between each data acquisition (see Figure 3b).
Figure 2. Timescale of a typical dioxin GC.
Figure 3a. Normal dual GC configuration: The gray area represents dead time, where no data is acquired.
Figure 3b. DualData Acquisition sequence. Only the relevant retention time windows of both GCs are monitored.
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Figure 6. Flow of the analytical column of GC 1 is guided into the MS and is acquired while the flow of GC2 is directed into waste (upper schematic) and vice versa (lower schematic).
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Figure 8. Example of peak integrity of dioxin trace analysis (Hexa CDD/F) using the DualData Acquisition.
native HxCDF (373.8202 m/z)
native HxCDF (375.8172 m/z)
13C12 Standard HxCDF (383.8633 m/z)
13C12 Standard HxCDF (385.8604 m/z)
native HxCDD (389.8151 m/z)
native HxCDD (391.8121 m/z)
13C12 Standard HxCDD (401.8554 m/z)
13C12 Standard HxCDD (403.8524 m/z)
Column: Thermo TR-Dioxin 60m x 0.25mm ID x 0.1µm film; Oven: 150 (2)-25-230(25)-6-320(6)
2,3,7,8-TCDDRT: 22.57 - 31.20
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Column: Thermo TR-Dioxin 60m x 0.25mm ID x 0.1µm film; Oven: 150 (2)-25-230(25)-6-320(6)
Figure 9. Separation of the 2,3,7,8-TCDD to the next eluting TCDD. The valley is below 25% as required by EPA1613.
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NL:7.60E5m/z= 327.8815-327.9015 MS D90311A20NL:4.34E5m/z= 405.7981-405.8181 MS D90311A20NL:3.09E5m/z= 485.7010-485.7210 MS D90311A20NL:3.47E5m/z= 563.6120-563.6320 MS D90311A20NL:3.93E5m/z= 643.5200-643.5400 MS D90311A20NL:1.97E5m/z= 721.4310-721.4510 MS D90311A20NL:1.97E4m/z= 799.3230-799.3430 MS D90311A20
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RT: 17.14AA: 181218
RT: 21.67AA: 202188
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NL:1.37E5m/z= 325.6574-326.0329 MS ICIS X4-210313-1S3
NL:8.59E4m/z= 327.7226-328.0981 MS ICIS X4-210313-1S3
NL:6.17E4m/z= 337.7672-338.1427 MS ICIS X4-210313-1S3
NL:3.98E4m/z= 339.6447-340.2079 MS ICIS X4-210313-1S3
Figure 10a. Example of PCB trace analysis (5Cl PCB)
Figure 10b. Example of PBDEs trace analysis.
DFS
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Figure 4. TRACE 1310 GC with DualData XL module (left front slot), standard SSL Injector (right front slot) and the Instant Connect Helium Saver Injector Module (back slots) mounted on the DFS Magnetic Sector GC-HRMS.
RESULTSThe analytical performance with DualData XL Acquisition and conventional GC/MS configuration was compared using the same set of polychlorinated dioxins and furans, PCBs and PBDE samples as model compounds. Sensitivity was compared by using low concentrated PCDD/PCDF standards as well as a low level pooled blood sample. The GC separation integrity, ruggedness and long-term stability of the column switching system have been proven in unattended sample. The Instant Connect Helium Saver Injector Module can be used on the DFS Magnetic Sector GC-HRMS in combination with the DualData XL Option.
The Instant Connect Helium Saver Injector Module is an innovative split/splitless (SSL) inlet system capable of dramatically reducing helium consumption.
Figure 7. Schematic flow diagram of the Instant Connect Helium Saver Injector Module during Injection (left side) and during data acquisition.
The septum purge and the split flow is substitute by nitrogen. During the injection, a small portion of nitrogen enters the analytical column but such as the solvent it will be guided into the service vacuum by the DualData XL flow switching system.
Figure 7. The module and injector components of the Instant Connect Helium Saver Injector Module.
