LudgerPureTM 2AB Labeled NA4 Glycan€¦ · Detector: Jasco FP920 Excitation wavelength: 360 nm...
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Transcript of LudgerPureTM 2AB Labeled NA4 Glycan€¦ · Detector: Jasco FP920 Excitation wavelength: 360 nm...
Certificate of Analysis
LudgerPureTM 2AB Labeled NA4 Glycan
Cat. # : CAB-NA4-01 Lot # : A4BJ-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
200
400
600 LudgerSepN1-A51Q-01-CAB-NA4-01-A4BJ-01 FluorescencemV
min
10.4
9
12.7
4
37.8
7
42.9
2
45.5
2
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NA4 glycan (Cat. #: CAB-NA4-01, Lot No. A4BJ-01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled NGA4 Glycan
Cat. # : CAB-NGA4-01 Lot # : A51E-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
200
400
600 LudgerSepN1-A51Q-01-CAB-NGA4-01-A51E-01 FluorescencemV
min
7.76
10.4
6
26.0
3
27.7
528
.97
30.5
7
37.2
4
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NGA4 glycan (Cat. #: CAB-NGA4-01, Lot No. A51E-
01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled A3 Glycan
Cat. # : CAB-A3-01 Lot # : A4AQ-02 Size : approx. 100 pmol
Purity: >90% by hplc (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
100
200
300 LudgerSepN1-A51Q-01-CAB-A3-01-A4AQ-02 FluorescencemV
min
7.77 40
.67
40.7
7 42.3
3
44.2
5
46.0
247
.47
49.1
9
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled A3 glycan (Cat. #: CAB-A3-01, Lot No. A4AQ-02).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled NA3 Glycan
Cat. # : CAB-NA3-01 Lot # : A4AT-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
125
250
450 LudgerSepN1-A51Q-01-CAB-NA3-01-A4AT-01 FluorescencemV
min
33.3
434
.76
38.0
0
42.9
2
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NA3 glycan (Cat. #: CAB-NA3-01, Lot No. A4AT-
01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled NGA3 Glycan
Cat. # : CAB-NGA3-01 Lot # : A51H-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
125
250
450 LudgerSepN1-A51Q-01-CAB-NGA3-01-A51H-01 FluorescencemV
min
7.77 10
.48
25.8
826
.61
27.6
6
31.0
6
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NGA3 glycan (Cat. #: CAB-NGA3-01, Lot No.
A51H-01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled NGA3 Glycan
Cat. # : CAB-NGA3-01 Lot # : A51H-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
125
250
450 LudgerSepN1-A51Q-01-CAB-NGA3-01-A51H-01 FluorescencemV
min
7.77 10
.48
25.8
826
.61
27.6
6
31.0
6
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NGA3 glycan (Cat. #: CAB-NGA3-01, Lot No.
A51H-01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled A2F Glycan
Cat. # : CAB-A2F-01 Lot # : A4AR-02 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-100
250
500
900 LudgerSepN1-A51Q-01-CAB-A2F-01-A4AR-02 FluorescencemV
min
39.3
6
43.3
944
.96
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled A2F glycan (Cat. #: CAB-A2F-01, Lot No. A4AR-02).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled A1F Glycan
Cat. # : CAB-A1F-01 Lot # : A4AJ-02 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
200
400
600 LudgerSep N1-A4AP-01-CAB-A1F-01-A4AJ-02 FluorescencemV
min
35.1
036
.03
37.5
238
.97
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled A1F glycan (Cat. #: CAB-A1F-01, Lot No. A4AJ-02).
HPLC Running Condition
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled NA2F Glycan
Cat. # : CAB-NA2F-01 Lot # : A531-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
200
400
600 LudgerSepN1-A52A-01-CAB-NA2F-01-A531-01 FluorescencemV
min
7.81
9.16
10.6
1
31.2
7
32.9
1
34.5
2
37.7
639
.02
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NA2F glycan (Cat. #: CAB-NA2F-01, Lot No. A531-
01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled NGA2F Glycan
Cat. # : CAB-NGA2F-01 Lot # : A51C-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
125
250
375
500 LudgerSepN1-A51Q-01-CAB-NGA2F-01-A51C-01 FluorescencemV
min
6.82 7.78
10.4
4
25.9
6
27.8
3
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NGA2F glycan (Cat. #: CAB-NGA2F-01, Lot No.
A51C-01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB labeled A1 Glycan
Cat. # : CAB-A1-01 Lot # : A4AR-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-100
250
500
900 LudgerSepN1-A51Q-01-CAB-A1-01-A4AR-01 FluorescencemV
min
33.0
8
36.0
7
37.8
6
39.6
4
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled A1 glycan (Cat. #: CAB-A1-01, Lot No. A4AR-01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled NA2 Glycan
Cat. # : CAB-NA2-01 Lot # : A4BP-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1).
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
125
250
375
500 LudgerSepN1-A51Q-01-CAB-NA2-01-A4BP-01 FluorescencemV
min
7.78
9.99
33.4
1
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NA2 glycan (Cat. #: CAB-NA2-01, Lot No. A4BP-01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled NGA2 Glycan
Cat. # : CAB-NGA2-01 Lot # : A4C2-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
125
250
450 LudgerSepN1-A51Q-01-CAB-NGA2-01-A4C2-01 FluorescencemV
min
7.76
8.27
10.4
4
24.7
725
.88
Figure 1 LudgerSepN1 HPLC profile of 2AB labeled NGA2 glycan (Cat. #: CAB-NGA2-01, Lot No. A4C2-
01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm.
Certificate of Analysis
LudgerPureTM 2AB Labeled MAN-9 Glycan
Cat. # : CAB-MAN9-01 Lot # : A4BO-05 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
100
200
300
400 LudgerSepN1-A4B9-01-CAB-MAN9-01-A4BO-05 FluorescencemV
min
7.39
42.5
2
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled MAN-9 glycan (Cat. #: CAB-MAN9-01, Lot No.
A4BO-05).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled MAN-8 Glycan
Cat. # : CAB-MAN8-01 Lot # : A532-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-30
0
25
50
80 LudgerSepN1-A522-01-CAB-Man8-01-A532-01 FluorescencemV
min
7.73
40.8
041
.56
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled MAN-8 glycan (Cat. #: CAB-MAN8-01, Lot No.
A532-01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled MAN-7 Glycan
Cat. # : CAB-MAN7-01 Lot # : A4BN-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
100
200
300 LudgerSepN1-A4B9-01-CAB-MAN7-01-A4BN-01 FluorescencemV
min
8.01 33
.24
36.6
8
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled MAN-7 glycan (Cat. #: CAB-MAN7-01, Lot No.
A4BN-01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled MAN-6 Glycan
Cat. # : CAB-MAN6-01 Lot # : A4BM-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-20
50
100
150
200 LudgerSepN1-A4B9-01-CAB-MAN6-01-A4BM-01 FluorescencemV
min
33.1
7
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled MAN6 glycan (Cat. #: CAB-MAN6-01, Lot No.
A4BM-01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled MAN-5 Glycan
Cat. # : CAB-MAN5-01 Lot # : A4BQ-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
200
400
600 LudgerSepN1-A51Q-01-CAB-MAN5-01-A4BQ-01 FluorescencemV
min
7.75
9.91
25.7
6
29.9
9
33.9
0
36.8
9
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled MAN-5 glycan (Cat. #: CAB-MAN5-01, Lot No.
A4BQ-01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm
Certificate of Analysis
LudgerPureTM 2AB Labeled Hybrid Glycan
Cat. # : CAB-HYBRID-01 Lot # : A51I-01 Size : approx. 100 pmol
Purity: > 90% pure as assessed by HPLC (see Fig 1)
5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50
125
250
375
500 LudgerSepN1-A51Q-01-CAB-HYBRID-01-A51I-01 FluorescencemV
min
6.78 7.77
10.4
6
33.3
0
Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled HYBRID glycan (Cat. #: CAB-HYBRID-01, Lot No.
A51I-01).
HPLC Running Conditions
Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C
Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4
Gradient: 0-75 min - 35-65 % B
75-80 min - 65-100 % B
80-83 min - 100 % B
83-85 min - 100-35 % B
85-115 min - 35 % B
Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm