Luc Montagnier Borovets October 23, 2013

8/13/2019 Luc Montagnier Borovets October 23, 2013

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Water structurescarrying DNA

information,Application to

HIV/AIDS and Autism

L.Montagnier, Borovets, October 23, 2013


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The Evolution of

Medicine

Toward 4 P Qualities

P1 Preventive

P2 PredictiveP3 Personalized

P4 Participative


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Emergence or reemergenceof new epidemics due to :

Globalization of exchanges and travels

Demography: concentration in large cities Nutrition (pesticides, water)

Environmental factors

Climate changes, electromagnetic radiations

Contacts with wild and farm animals

Decline of immune defenses


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- Cancers

- Cardiovascular- Neurodegenerative

- Arthritic

- Autoimmune

- Multifactorial, but in common:

oxidative stress

infectious agents (?)

The most important burden

Chronic Diseases


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Radiations (, X, UV, visible, hertzian)

Air chemical pollution

Inadequate Food

Excessive physical exercize

tobacco smoke

alcohol

ischemia

Parasitic infections

Bacterial infections

Viral infections

Various environmental factors effects

accumulate

+++

++

+

+

+

= DISEASE

+


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Lipid hyperoxydation (plasma, cell membranes)

Protein Oxydation --> destruction, agregates

DNA oxydation --> Mutations, Chromosomal

Breakage

Pathological effects of Oxydative Stress


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Oxidative stress

Weakens the immune system

Activates transcription factors

(NF-kappa B)

Activates genes involved

in cell division, inflammatory cytokines,

lymphocytes activation

Immune dysfunction, apoptosis

(TH1 TH2)


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The Human Genome

Genes(+ introns +promoters) 3%

Retroelements:retroviruses 8%

retrotransposons 13%

LTR 23%Total 47%

Repeats 50%

LINE 22%

SINE 13%

Unknown ?


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Extreme genetic plasticityof microorganisms:

virus : HIV, Influenza Bacteria (plasmids)

Parasites (retrotransposons)

against reaction of the immune system


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- Biofilms

- Mobile antibiotic resistant genes- Nanoforms

- Nanostructures

- Endosymbiontes

How bacteria have learned how to

persist despite the immune system


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Parasite Bacterium Virus

(Worm) intracellular

Filarial nematode Wolbachia


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Persistent cold infections

Antibiotics affect them only when they go out of sanctuaries

Inability of the immune system to eradicate them

(tolerance, oxidative stress)

Non-multiplicative forms of classical bacteria

intracellular bacteriaSanctuaries

(bone marrow, joints, intestine, brain, .)

Vectors (Parasites)

Remote effects (toxin, nanostructures)


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EXPLORING THE ROLEOF LATENT INFECTION

IN CHRONIC DISEASESA physical and molecular approach


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Two technologies for detecting

bacteria and viral DNAs

A new technology based on the

production of electromagnetic waves

The Polymerase Chain Reaction

(PCR) using the 16S ribosomal DNA

variability


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oligo

oligo

Taq

polymerase

Classical model of PCR


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Resonance emission of low frequency

electromagnetic waves by high water dilutions

of DNA.

A newly discovered propertyof DNA :


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Molecular recognition

without close contact(by waves and resonance)


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Capture of the signals

Amplifier Computer

X 500 Signal Analysis

software

Sensor coil

Sample


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Detection of Ultra Low Frequencies

Waves (ULF 500-2000 hertz) in certain

dilutions of filtrates (100nM, 20nM,15nM) from cultures of micro-

organisms (virus, bacteria) or from the

plasma of humans infected with the

same agents.

FACTS


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2ng/1ml 10-2 10-3 10-4 10-5 10-6 10-7 10-8 10-9 10-10 10-12 10-13 10-14 10-15 10-16

Filtrati

on 0.1

0.02

7-100 Hz

1000 -

3000 Hz


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Time (sec)

Amplitude

Noise

(+)


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Spectral Frequency Analysis

Fourier

Tranformation

A positive signal is defined by:

amplitude increase

Shift to higher frequencies (500-2000 Hertz)


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Noise

(+)

Frequency (1-20000 Hertz)


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Suspensions of pure culture of bacteria with pathogenic

potential are producing electromagnetic signals (EMS) in the

range of dilution usually 10-810-13, sometime (E.coli) up to

10-18

They are:E.coli, Streptococcus, Salmonella, Staphylococcus,

B.subtilis, Clostridium, Pseudomonas, etc.


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I DNAs emit EMS

II EMS are produced by water nanostructures (naneons)

III EMS are producing naneons

IV Naneons and EMS carry specific DNA information


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EMS are produced by water

nanostructures (naneons)

Evidence :

from filtration

Size : between 20 and 100 nM for bacterial sequences

Smaller that 20M for viral sequences

from biophysical studies indicating spectrometral changes in the dilutions

producing EMS


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Naneons and EMS carry

specific DNA information

Natural and digital transmission


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oligo

oligo

Taq

polymerase

Classical model of PCR


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DNA WaterNaneons

EMS EMS

DNA

Water

Naneons

PCR

7 Hz


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-6

metal

Generator 7Hz

+ + +

-2 -3 -4 -5 -6

18hrs

water-6

Tube 2

water

EMS

Tube 1

DNA


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D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after f iltration 450 and 20 nM

Transmission in water of D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM

DW: Distilled Water / FD2: Dilution 10-2 after filtration 450and 20 nM


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DNA

Water

Naneons

EMS

DNA

Water

Naneons

PCR

Computer

Digitized

Receiver

ComputerEMS Analog

Water-mediated photonic transmission of DNA


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Reproduction of DNA transduction

in other laboratories

File EMS of 194 bp DNA from HIV1 LTR

Sent to Benevento University,

Molecular Biology Laboratory

DNA reproduced and sequenced

100 % identical to original

File EMS of 499 bp DNA from Borrelia burgdorferiSent to Laboratory of Chronix Biomedicals

University of Gottingen

Molecular Biology LaboratoryDNA reproduced and sequenced


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100 % identical to original

File EMS of 499 bp DNA from Borrelia burgdorferi

Sent to Laboratory of Chronix BiomedicalsUniversity of Gottingen


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Water-mediated photonic transmission of DNA

Gel electrophoresis of the PCR DNA product (Borrelia Burgdorferi)

E.Schutz et al. Goettingen, 2011


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How pathogenic information can persist,and escape immune defence and treatment.

DNA

DNADNA

DNA

DNA

WATER(plasma)

ELF


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- The viral DNA reservoir- The Higher sensitivity of Africanpeople to HIV infection

Two main problems for AIDS researchin 2013


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The dual origin of EMS in HIV/AIDS

EMS plasma RBC

HIV


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Evidence that the agent X is a bacterium

- Presence of a sequence close to 16 S ribosomal DNA

of Rickettsiales

- Sensitive to antibiotics ( in vitro and in vivo)

- Growth in cell line

- Electron microscopy

- Associated with Red Blood Cells


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Human sequences associated with

the agent X

237 bp (human chromosome 1)

213 bp (human chromosome 7)

and more


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Strong correlation with HIV infection

- Contains human DNA sequences emitting EMS

only in pathogenicHIV infection

-Sequence 213 bp from Chromosome 7

EMS in HIV + patients

no EMS in HIV - patients


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Risk factor 2RBC

Risk factor 1

RBC

HIV plasma

RBC

DNA

PCR EMS+ +

++

-+

RISK FACTORS OF HIV INFECTION

(all HIV)

(20 nM)

(213/237 bp) (100 nM)(HIV+)( all HIV+, a few HIV-)

( all HIV+, many HIV-)(HIV- & HIV+)(100 nM)

(LTR 195 bp)


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CONCLUSION 2

Origin of EMS :

not necessarily depending on DNA sequence

- depending on transmissible modification of DNA

(free radicals of water ?)

- Association with pathogenicity


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GUT BLOOD BRAIN


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se Correlation between EMS antibiotic treatment


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20 -

30 -

10 -

40 -

%EMS/nois

50 100 133270 -

days

Correlation between EMS, antibiotic treatment

and clinical signs in an autistic child

AZITHROMYCIN CEFUROXIM

Acceptvariousfood

Moreprese

ntwith

Familymem

bers

Startsspeaking


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Environment

al factorsgenetic

susceptibility

immunosuppression

Oxidative

stress

Bacterial

agentsOxidative

stress

prion effect

Less and less

reversibleReversible

Somaticmutations


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In press

Electromagnetic detection of HIV DNA in the blood of AIDS patients

treated by antiretroviral therapy.

Luc MONTAGNIER^, Jamal AISSA, Claude LAVALLEE,

Mireille MBAMY, Joseph VARON#, and Henri CHENAL

World Foundation for AIDS research and Prevention (UNESCO), 1 rue Miollis, 75015, Paris, France Nanectis Biotechnologies, France

CIRBA Centre Intgr de Recherches Biocliniques dAbidjan, Ivory Coast# The University of Texas Health Science Center, Houston, USA.

Abstract : Electromagnetic signals of low frequency have been shown to be durably produced in aqueous dilutions of the

Human Imunodeficiency Virus DNA. In vivo, HIV DNA signals are detected only in patients previously treated byantiretroviral therapy and having no detectable viral RNA copies in their blood. We suggest that the treatment of AIDS

patients pushes the virus towards a new mode of replication implying only DNA, thus forming a reservoir insensitive to

retroviral inhibitors. Implications for new approaches aimed at eradicating HIV infection are discussed.

Key words: DNA, Electromagnetic signals, bacteria

Centre Intgr de Recherche Bioclinique dAbidjan


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g q j


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World Foundation of AIDS

Research and Prevention

R. Olivier, Cl. Lavallee,H.Chenal, M. Mbamy,

Nanectis Biotechnologies SAJ.Aissa, Cl.Lavallee, R.Olivier

Goettingen University and Chronix

BiomedicalsE. Schutz, H.Urnovitz

University of Washington, SeattleGerald H. Pollack


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Absence of evidenceis not

evidence of absence

Carl Sagan

Luc Montagnier Borovets October 23, 2013

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Transcript of Luc Montagnier Borovets October 23, 2013