Laser2000_Biotech+Analytical

Bulletin No. 3011 www.laser2000.de

& Analytical Instrumentation

Fluorescence Filters Edge FiltersNarrowband Laser-line FiltersNotch FiltersHigh-Performance MirrorLasersOptical InstrumentationOptics & Optomechanics

Optical Products for Biotech

Call us: +49 (0)8153 405-0

For the fastest fluorescence measurements and “no burn-out”

Laser Notch Filters . . . . . . . . . . . . . . . . . . . . . . .42T

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Deep Laser-blocking Filters

Single-notch Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42Multi-notch Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45

405 nm – 830 nm

Raman Edge Filters . . . . . . . . . . . . . . . . . . . . . . .32

Laser-line Filters . . . . . . . . . . . . . . . . . . . . . . . . . .36

Laser Diode Clean-up Filters . . . . . . . . . . . . . . .41

Ultra-broadband High-performance Mirror . . .47

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Laser-line filters

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What’s Inside

Fluorescence Filters and Sets . . . . . . . . . . . . . . . .4T

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BrightL

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Razor sharp – for the most discriminating Raman measurements

248 nm – 1064 nm

Clean up your laser with ultra-fast roll-off and the highest peak transmission – don’t waste expensive laser light!

266 nm – 1064 nm

High transmission, low ripple, and exceptional noise blocking for the cleanestdiode laser output

375 nm – 635 nm

The most versatile high-performance mirror on the market – there is nothingelse like it!

350 nm – 1100 nm in just one mirror

For more innovative filters and pioneering OEM solutions,see www.semrock.com.

Single-band Filter Sets . .6Multiband Filter Sets . . . .6UV Filters . . . . . . . . . . . .10Multiphoton Filters . . . .20

Clinical Filter Sets . . . . . . . . . .22Bandpass Filters . . . . . . . . . . .24Dichroic Beamsplitters (45°) .28

NEW – UV Optical Filters!Filters Made with Only Hard Coatings

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Welcome to the latest LASER 2000 cataloguefor Biotechnology and Analytical Instrumentation...In this issue we focus on the industry-leading filters from Sem-rock, which are brighter and spectrally more sophisticated than any other filter. In addition we give you a short selection of other superior and very useful products for these applica-tions like lasers, sensors, optics and optomechanics.

When dealing with Laser 2000, you will receive advice and in-formation from trained engineers and physicists, whose tech-nical expertise is of the highest level. With products in service at thousands of locations all over the world, you can be sure that LASER 2000 will provide you with the performance and reliability that you need. Contact us today to find out how you can benefit from our technology.

Victoria BenediktSales Assistance+49 (0) [email protected]

Gabriela ThunigSales Assistance+49 (0) [email protected]

Dr. Stefan KremserProduct Specialist+49 (0) [email protected]

Mark DrechslerProduct Specialist+49 (0) [email protected]

Bernhard DaunerProduct Specialist+49 (0) [email protected]

Sales Assistance

Product Specialists

Dr. Helge BrüggemannProduct Specialist+49 (0) 30 962 [email protected]

Dr. Georg DraudeProduct Specialist+49 8153 [email protected]

Lasers

Femtosecond Lasers ............................................................................49

Diode pumped solid state lasers ........................................................50

Deep UV Gas Lasers .............................................................................52

Digital Detector Controllers .................................................................53

Spectrally Programmable Light Engine .............................................58

Optical Instrumentation

Silicon Solutions for Low Light Detection .........................................54

Spectroscopy Cameras ........................................................................54

NIR InGaAs Spectrometers..................................................................56

UV-VIS Spectrometers .........................................................................57

Optics & Optomechanics

Optics and Optomechanics .................................................................60

Laser Safety Goggles ...........................................................................60

Service

Free Catalogue Order ...........................................................................61

Anette HartlSales Assistance+49 8153 [email protected]

2 Call us: +49 (0)8153 405-0

IntroductionIntroduction Introduction

Semrock Hard-coated Ion Beam Sputtered Optical FiltersThe Standard in Optical Filters for Biotech & Analytical Instrumentation

Semrock introduced a revolutionary optical filter technology to Biotech and Analytical Instrumentation. For more thansix years, Semrock has been successfully combining the most sophisticated and modern ion-beam sputtering depositionsystems, renowned for their stability, with its own proprietary deposition control technology, unique predictive algorithms, processimprovements and volume manufacturing capability. The result isoptical filters of unsurpassed performance and reliability that haveset the standard for the biotech and analytical instrumentationindustries.These filters are so exceptional that they are award-winning and so innovative that they are patented. And Semrocknever stops improving.

That is why Semrock consistently delivers:

The brightest filters with the most discriminating spectra forthe fastest measurements

Proven reliability for permanent performance; see page 3

Unsurpassed batch-to-batch reproducibility for assured supply

We are your Biotech & Analytical Instrumention Optics Experts

Semrock knows optics! We routinely do optical ray tracing, run Monte Carlo simulations, perform stray light analysis,and of course craft sophisticated optical filter designs. Why is all this necessary? We believe that in order to designthe best optical filters, great filter technology is not enough – we must also thoroughly understand your applications.And because we have long-focused all of our resources on the biotech and analytical instrument markets, we have aprofound understanding of our customers’ needs. We thoroughly understand optics, we bring you great filters, andwe also speak your language!

Product lines:

Fluorescence FiltersFluorescence microscopy, DNA microarray scanners, high-throughputand high-content screening, flow cytometry, real-time PCR, microplatereaders, electrophoresis scanners, fluorescence imagers, chemicalprocess monitoring . . .

Raman Spectroscopy FiltersRaman microscopes, laboratory spectrometers, Raman microprobe analyzers (including optical fiber probes) . . .

Biomedical Laser OpticsOphthalmological, dermatological, and other medical laser systems,laser-based scanners, multiphoton fluorescence systems and microscopes . . .

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Four different batches; identical results!

Product SpecialistsBernhard Dauner+49 (0) [email protected]

Sales Assistance Victoria Benedikt+49 (0) 8153-405-61 [email protected]

Introduction

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Introduction

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Example of bandpass filter spectra measured beforeand after ten 24-hour cycles of Aggravated Humidity testing according to MIL-STD-810F, demonstratingnegligible change.

Semrock’s dedicated reliability laboratory with state-of-the-art environmental test chambers is in regular use.

No measurable change!

All Semrock filters demonstrate exceptional reliability. The simple all-glass structure and hard dielectric glass coatings(as hard as the glass on which they are coated!) mean they are virtually impervious to humidity-induced shifting and canbe cleaned and handled like any standard glass optics. All Semrock coatings are hard – never soft.

All Semrock filters are capable of withstanding high optical intensities. Semrock filters either have laser-damagethreshold specifications (see page 46) or, depending on the application, have been tested with intense broadbandsources for prolonged periods of time with no noticeable degradation. Semrock filters do not burn out under normal conditions, even with prolonged use and with ultraviolet (UV) irradiation.

You will never find any adhesives in the optical path of any Semrock filter. Epoxies and cements can absorb watervapor and swell or shift, and they can photo-darken or be optically damaged. Semrock filters are built for longevity.

Semrock filters have been tested to meet or exceed requirements for environmental and physical durability set forthin specifications such as MIL-STD-810F, MIL-C-48497A, MIL-C-675C, and ISO 9022-2. The table below shows someof the key standards against which our filters are regularly tested.

Environmental Durability Testing Standard / Procedure Test Description

Humidity MIL-STD-810F (507.4) Aggravated Humidity (> 10 x 24 hr cycles)High Temperature MIL-STD-810F (501.4) Induced Hot (> 7 x 24 hr cycles)Low Temperature MIL-STD-810F (502.4) Cold (C2) (24 hr cycles)

Semrock Hard-coated Ion Beam Sputtered Optical Filters

Proven Reliability

Physical Durability Testing Standard / Procedure Test Description

Adhesion MIL-C-48497A (4.5.3.1) “Tape Test” Humidity MIL-C-48497A (4.5.3.2) Damp HeatModerate Abrasion MIL-C-48497A (4.5.3.3) “Cheesecloth Test” (> 50 cycles) Solubility/Cleanability MIL-C-48497A (4.5.4.2) Sequential immersion in acetone and alcohol Water Solubility MIL-C-48497A (4.5.5.3) Immersion in distilled water (> 24 hrs)

RoHS Compliance: Semrock products are compliant, without exemptions, with EU Directive 2002/96/EC, Restrictive Use of Hazardous Substances (RoHS). Semrock has instituted controls to ensure our suppliers provide only RoHS compliant materialsfor our products.

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Fluorescence FiltersFluorescence Filters

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The award-winning BrightLine series of optical fluorescence filters has revolutionized fluorescence instruments – frommicroscopes to microarrays. That’s why leading OEMs around the world have designed BrightLine filters into their fluorescence systems.

Semrock adapted and improved the premier optical coating technology in the industry, Ion Beam Sputtering (IBS), tosolve the toughest fluorescence problems. By combining this state-of-the-art technology with our deep understandingof fluorescence applications we have produced the brightest, most durable fluorescence filters available anywhere. In fact BrightLine filter technology is so innovative that it is patented (U.S. Patent No. 6,809,859 and pending).

When You Want the Best!

BrightLine® Fluorescence Filters

“I got the filters, but you forgot to include the sunglasses! These are the brightest filters I have ever seen!! Kudos.”

Michael W. DavidsonMolecular Expressions™National High Magnetic Field LaboratoryThe Florida State University

Photos courtesy Mike Davidson at Molecular Expressions,using BrightLine® fluorescence filter sets.

BrightLine Filters are Bright

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Typical GFP-3035B Filter Set for Green Fluorescent Protein

BrightLine filters are perfectly optimized for fluorescence applications. Semrock’s state-of-the-art ion-beam sputtering technology combined with its patented single-substrate filter construction result in the highest transmission on the market.And steeper edges, precise wavelength accuracy and carefully optimized blocking mean better contrast and faster measurements – plus affordable “ZEROpixel shift” performance is available from stock!

The Standard in Optical Filters for Biotech & Analytical Instrumentation > 100,000 Ion Beam Sputtered filters delivered – extensive inventory now!

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Proven BrightLine Durability (see page 3)

All BrightLine filters: hard dense glass coatings on hard glass substrates

Can be cleaned and handled, even with acetone!

Impervious to humidity, insensitive to temperature

No soft coatings – no exceptions

All BrightLine filters: no “burn-out,” no annual replacement

Stand up to intense Xenon and Mercury arc-lamp and halogen light sources

No adhesives in the optical path to darken or degrade

Made with the same refractory materials as our high “laser damage threshold” laser optics

Semrock is continuously improving its already cutting edge Ion Beam Sputtering technology, regularly introducing new state-of-the-art filters for the most discriminating researcher.

• New ultraviolet (UV) fluorescence filters (see pages 6 & 10) –efficiently access wavelengths below 300 nm!

• Amazing multiband filters sets (see page 15) – see what you’ve been missing!

• Multiphoton filters (see page 20) that will astonish even the most discerning researchers

Cutting-edge Research Offerings

With Ion Beam Sputtering manufacturing prowess second-to-none, and after years of continuous improve-ment, Semrock recently introduced the famous durabilityand consistency of its hard-coated BrightLine technologyfor the mainstream clinical market. Our spectacularBrightLine Clinical™ series of fluorescence filters (see page 22) are optimized specifically for the cost-sensitive clinical user. In field trials by independent clinical laboratory personnel, BrightLine Clinical filtersets were found to have significantly better performancethan even the best conventional soft-coated filter sets,yet BrightLine Clinical filters do not burn out ordegrade with use, and are fully cost-competitive!

Superior Clinical Offerings

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BrightLine FF01-280/20 UV filterTraditional metal-dielectric filter

BrightLine Clinical™ Fluorescence Filter SetsHard-coated performance at a soft-coated price™

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Proven BrightLine Durability (see page 3)

All BrightLine filters: hard dense glass coatings on hard glass substrates

Can be cleaned and handled, even with acetone!

Impervious to humidity, insensitive to temperature

No soft coatings – no exceptions

All BrightLine filters: no “burn-out,” no annual replacement

Stand up to intense Xenon and Mercury arc-lamp and halogen light sources

No adhesives in the optical path to darken or degrade

Made with the same refractory materials as our high “laser damage threshold” laser optics

Semrock is continuously improving its already cutting edge Ion Beam Sputtering technology, regularly introducing new state-of-the-art filters for the most discriminating researcher.

• New ultraviolet (UV) fluorescence filters (see pages 6 & 10) –efficiently access wavelengths below 300 nm!

• Amazing multiband filters sets (see page 15) – see what you’ve been missing!

• Multiphoton filters (see page 20) that will astonish even the most discerning researchers

Cutting-edge Research Offerings

With Ion Beam Sputtering manufacturing prowess second-to-none, and after years of continuous improve-ment, Semrock recently introduced the famous durabilityand consistency of its hard-coated BrightLine technologyfor the mainstream clinical market. Our spectacularBrightLine Clinical™ series of fluorescence filters (see page 22) are optimized specifically for the cost-sensitive clinical user. In field trials by independent clinical laboratory personnel, BrightLine Clinical filtersets were found to have significantly better performancethan even the best conventional soft-coated filter sets,yet BrightLine Clinical filters do not burn out ordegrade with use, and are fully cost-competitive!

Superior Clinical Offerings

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BrightLine Clinical™ Fluorescence Filter SetsHard-coated performance at a soft-coated price™

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Semrock carries a broad range of high-performance no “burn-out” fluorescence microscopy filter sets that will satisfy even themost demanding researcher. Single-band sets are available with an affordable “zero pixel shift” option that is ideal for making precise multi-color composite images (see page 13). Or choose from our amazing multiband filter sets for simultaneous and high-speed sequential imaging of samples with multiple fluorophores (see page 15).

Research Microscopy Filter Sets(For stunning, yet affordable, Clinical Microscopy Filter Sets, see page 22.)

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[1] Single-band Set consists of a single-band exciter, single-band emitter, and single-edge dichroic beamsplitter; “Full Multiband” Set consists of multiband exciter, multibandemitter, and multiband dichroic beamsplitter; “Pinkel” Set consists of single-band exciters, multiband emitter and multiband dichroic beamsplitter; “Sedat” Set consists ofsingle-band exciters, single-band emitters, and multiband dichroic beamsplitter. See the Technical Note on page 19 for more information.

[2] For a complete list of fluorophores and filter compatibility, go to www.semrock.com.

Blue: DAPI, Hoechst, AMCA, Alexa Fluor 350Green: FITC, GFP, rsGFP, BODIPY, Alexa Fluor 488Orange: TRITC, Cy3, Texas Red®, MitoTracker Red, Alexa Fluor 568 & 594Red: Cy5™, APC, TOTO-3, TO-PRO-3, Alexa Fluor 647 & 660

Green: GFP, rsGFP, FITC, Alexa Fluor 488Red: DsRed, TRITC, Cy3, Texas Red®, Alexa Fluor 568 & 594

Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1Yellow: YFP, Calcium Green-1, Fluo-3, Rhodamine 123Red: HcRed, Cy3.5, Texas Red®, Alexa Fluor 594

Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor 350Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor 488Red: Texas Red®, MitoTracker Red, Alexa Fluor 568 & 594

Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1Yellow: YFP, Calcium Green-1, Eosin, Rhodamine 123

NEW!

NEW!

All in Stock – Only at Semrock!

Emission Full SetColor Prefix Type of Set [1] Primary Fluorophores [2] Page

Single-band Filter Sets

TRP-A- Single-band Tryptophan – New UV set 10

DAPI-1160A- Single-band DAPI, Hoechst, AMCA, BFP, Alexa Fluor 350 – Highest contrast set 10

DAPI-5060B- Single-band DAPI, Hoechst, AMCA, BFP, Alexa Fluor 350 – Highest brightness set 10

CFP-2432A- Single-band CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 10

FURA2-B- Single-band Fura-2 Ca2+ indicator, LysoSensor Yellow/Blue 11

GFP-3035B- Single-band GFP, EGFP, DiO, Cy2, YOYO-1, YO-PRO-1 11

FITC-3540B- Single-band FITC, rsGFP, Bodipy, FAM, Fluo-4, Alexa Fluor 488 11

YFP-2427A- Single-band YFP, Calcium Green-1, Eosin, Fluo-3, Rhodamine 123 11

TRITC-A- Single-band TRITC, Rhodamine, DiI, 5-TAMRA, Alexa Fluor 532 12

Cy3-4040B- Single-band Cy3, DsRed, PE, TAMRA, Calcium Orange, Alexa Fluor 546 & 555 12

TXRED-4040B- Single-band Texas Red®, Cy3.5, 5-ROX, Mitotracker Red, Alexa Fluor 568 & 594 12

Cy5-4040A- Single-band Cy5™, APC, DiD, Alexa Fluor 647 & 660 12

Dual-band Filter Sets

CFP/YFP-A- “Full Multiband” 16

CFP/YFP-2X-A- “Pinkel”

CFP/YFP-2X2M-A- “Sedat”

GFP/DsRed-2X-A- “Pinkel” 17

GFP/DsRed-2X2M-B- “Sedat”

GFP/HcRed-2X-A- “Pinkel” Green: GFP, rsGFP, FITC, Alexa Fluor 488 17Red: HcRed, Cy3.5, Texas Red®, Alexa Fluor 594

Triple-band Filter Sets

BFP/GFP/HcRed-3X-A- “Pinkel” Blue: BFP, DAPI, Hoechst, AMCA, Alexa Fluor 350 17Green: GFP, rsGFP, FITC, Alexa Fluor 488Red: HcRed, Cy3.5, Texas Red®, Alexa Fluor 594

CFP/YFP/HcRed-3X-A- “Pinkel” 16

CFP/YFP/HcRed-3X3M-A- “Sedat”

DA/FI/TX-B- “Full Multiband” 15

DA/FI/TX-3X-A- “Pinkel”

DA/FI/TX-3X3M-A- “Sedat”

Quadruple-band Filter Sets

DA/FI/TR/Cy5-4X-A- “Pinkel” 18

DA/FI/TR/Cy5-4X4M-B- “Sedat”


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Microscope Cubes and Sets Mounted in Cubes (also known as holders)

For a nominal amount more you can experience certified “zero-pixel-shift” performance. BrightLine ZERO™single-band fluorescence filter sets are affordable and in stock. Just append –ZERO. For example,TRITC-A-NTE-ZERO is the TRITC set in “ZERO” form mounted in a Nikon TE2000 cube. Leica single-band sets are only available as–ZERO sets. See page 13 for more information on BrightLine ZERO™ filters.

Leica, LDMK Leica, LLC Leica, LSCNikon, NTE Nikon, NQF Olympus, OMF Zeiss, ZHE Zeiss, ZAT

[1] See page 6 and page 23 for <Full set prefix>.

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Microscope Brand/ Cube Number for Filter SetCompatible Microscopes Designation for Cube Mounted in Cube

Nikon

TE2000, 80i, 90i, 50i, and any using the TE2000 NTE <Full set prefix>-NTEEpi-fluor Illuminator

E200, E400, E600, E800, E1000, TS100, TS100F, TE200, Quadfluor NQF <Full set prefix>-NQFTE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

Olympus

AX, BX, and IX series U-MF2 OMF <Full set prefix>-OMF

MVX10 (and other large optical path microscopes) U-MF/XL Contact Semrock Contact Semrock

Zeiss

Axio Imager, Axioplan2i, Axioplan2ie, Axiovert200, FL CUBE EC P&C ZHE <Full set prefix>-ZHEand Axioskop2 (post-2001) (Push-and-Click)

Axioplan (pre-version 2), Axiovert100, and Threaded ZAT <Full set prefix>-ZATAxioskop2 (pre-2002) Filter Cube

Leica

DM-4000, DM-5000, and DM-6000 DM-K LDMK <Full set prefix>-LDMK-ZERO

DM-LB, DM-LM, DM-LP, DM-RB, and DM-R HCRF4 DM-R LLC <Full set prefix>-LLC-ZERO

DM-IL, DM-IRB, DM-LS, DM-LSP, DM-R HCRF8, DM-IRB LSC <Full set prefix>-LSC-ZEROand DM-R XARF8



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[1] For transmission specifications for UV and multiband filters, go to www.semrock.com.[2] Filter performance is likely to remain satisfactory for Cone Half Angles as large as 10° for exciters and emitters, and 3° for dichroics.

Common Specifications (for Single and Multiband Sets)


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Orientation of Filters in a MicroscopeBecause BrightLine filters are so durable, you can easily populate your own cubes, sliders, and filter wheels without havingto worry about damaging the filters. To obtain the most optimal performance from the filters, they should be orientedproperly. The diagrams below explain the proper orientation.

The exciter and emitter shouldbe oriented so that the arrowon the side of the aluminumring points in the direction ofpropagation of the desired light– from the light source todichroic for the exciter andfrom the dichroic to eye orcamera for the emitter.Thedichroic must be oriented suchthat the reflective coating sidefaces toward the exciter or lightsource and the sample.

When viewing the dichroic with the reflective coating sidedown, you can see a double-reflection of a bright objectand the thickness of the filterat the far edge is apparent.

When viewing the dichroic with the reflective coating side up, you can see a predominantly single reflectionof a bright object and thethickness of the filter at the far edge is not visible.

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T E C H N I C A L N O T E

Averaged over passband (emitter band only for dichroic)

Property Exciter Emitter Dichroic Comment

Passband Transmission[1] Guaranteed > 93% > 93% > 90%Typical > 97% > 97% > 93%

Dichroic Reflection N/A N/A > 98% Averaged over exciter band(s)Angle of Incidence 0° ± 5° 0° ± 5° 45° ± 1.5° Range of angles over which optical specifi-

cations are guaranteed for collimated lightCone Half Angle [2] 7° 7° 2° For uniformly distributed non-collimated lightAutofluorescence Low Low Ultra-lowTransverse Dimensions 25.0 mm 25.0 mm 25.2 x Filter sizes shown are standard for most

35.6 mm Nikon, Olympus, and Zeiss microscopes.For Leica , see www.semrock.com.

Transverse Dimension Tolerance + 0.0 / – 0.1 mm ± 0.1 mm Thickness 5.0 mm 3.5 mm 1.05 mm FF01-357/44-25 is 5.0 mm thickThickness Tolerance ± 0.1 mm ± 0.05 mmClear Aperture > 21 mm > 22 mm > 80% Area over which all optical specifications

are metEdge Chipping < 0.1 mm Measured from substrate edgeRing Housing Material Aluminum, black-anodized Exciter and emitter only; Leica filters are not

mounted in aluminum ringsSurface Quality 60-40 scratch-dig Measured within clear apertureCoating Type “Hard” ion-beam-sputteredBlocking BrightLine filters have blocking far exceeding OD 6 wherever needed to ensure the blackest

background, even when using modern low-noise CCD cameras. The blocking is optimizedfor microscopy applications using our exclusive SpecMaker™ filter design software.

Reliability and Durability Ion-beam-sputtered, hard-coating technology with epoxy-free, single-substrateconstruction for unrivaled filter life. BrightLine filters are rigorously tested and proven to MIL-STD-810F and MIL-C-48497A environmental standards; see page 3 for details

Filter Orientation Arrow on ring indicates direction of propagation of light; see diagram belowMicroscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, and Zeiss microscopes.

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Ultraviolet (UV) Fluorescence Applications

Many biological molecules of interest naturally fluoresce when excited by shorter-wavelength UV light. This “intrinsic fluorescence” can be a powerful tool. One important application is the direct fluorescence imaging of aromatic amino acids including tryptophan, tyrosine, and phenylalanine, which are building blocks for proteins.The aromatic rings in these molecules give rise to strong fluorescence excitation peaksin the 260 to 280 nm range. Because the fluorescence is intrinsic, samples can beobserved without the added chemistry and limitations associated with “indirect” labeling by extrinsic fluorophores. Another important application is DNA quantitation.Purines and pyrimidines – bases for nucleic acids like DNA and RNA – have strongabsorption bands in the 260 to 280 nm range.The neurotransmitter serotonin alsoexhibits strong absorption at these wavelengths and associated intrinsic fluorescence.

Semrock’s UV BrightLine fluorescence filters offer a new and powerful tool for practic-ing direct fluorescence imaging. These new UV filters are both reliable (no “burn-out”)and offer performance nearly comparable to that of visible and near-IR filters. The topfigure shows the spectrum of a high-reliability 280 nm BrightLine excitation filter withthe highest commercially available transmission (> 70%), remarkably steep edges, andwideband blocking across the entire UV and visible spectrum. This spectrum is directlycompared to that of a traditional and inferior metal-dielectric filter. In one example system this filter difference was shown to provide well over 100x improvement in signal-to-noise ratio.

The bottom figure shows the spectra from a new UV filter set designed for imagingtryptophan, overlaid on the absorption and emission spectra of that amino acid. Notethe nearly ideal overlap and high transmission of all three filters in this set.


Introduction to Fluorescence filters

Optical fluorescence occurs when a molecule absorbs light at wavelengths within its absorption band, and then nearlyinstantaneously emits light at longer wavelengths within its emission band. For analytical purposes, strongly fluorescingmolecules known as fluorophores are specifically attached to biological molecules and other targets of interest to enablequantification, identification, and even real-time observation of biological and chemical activity. Fluorescence is widelyused in biotechnology and analytical applications due to its extraordinary sensitivity, high specificity, and simplicity.

Most fluorescence instruments, including fluorescence microscopes, are based on optical filters.A typical system hasthree basic filters: an excitation filter (or exciter), a dichroic beamsplitter, and an emission filter (or emitter).The exciter is typically a bandpass filter that passes only the wavelengths absorbed by the fluorophore,thus minimizing excitation of other sources of fluorescence and blocking light in the fluorescence emission band.The dichroic is an edge filter used at an oblique angle of incidence (typically 45°) toefficiently reflect light in the excitation band and to transmit light in the emission band.The emitteris typically a bandpass filter that passes only the wavelengths emitted by the fluorophore andblocks all undesired light outside this band – especially the excitation light.

In most fluorescence instruments, the best performance is obtained with thin-film filters, which are comprised of multiple alternating thin layers of transparentmaterials with different indexes of refraction on a glass substrate.The complex layer structure determines the spectrum of light transmission by a filter.Thin-film filters are simple to use, inexpensive,and provide excellent optical performance: high transmission over an arbitrarilydetermined bandwidth, steep edges, and high blocking of undesired light over the widest possible wavelength range. Recent advances in thin-film filter technology,unique to BrightLine filters, permit even higher performance while resolving thelongevity and handling issues that can affect filters made with older soft-coating technology.


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TRP-2044A single-band fluorescence filter set is idealfor imaging tryptophan (see page 10).

New BrightLine FF01-280/20-25 filter (see page 24).

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DAPI-5060B – Highest Brightness

Highest Contrast DAPI set on the market. The preferred DAPI set for most applications, this filter set is optimized for achieving very low noise while maintaining excellent brightness, resulting in unsurpassed contrast. The signal-to-background ratio achieved with this DAPI set forimaging typical nuclear-stain samples is more than twice that of a leadingcompetitor’s.

Brightest DAPI set on the market. This filter set is recommendedfor those special cases in which it is critical to achieve the highest possible brightness for the shortest possible exposure times, or for very low fluorophore concentrations. For shorter exposure times, theset exhibits up to 5 times more brightness, but has lower contrast thanthe DAPI-1160A set.

Exciter Part Number . . .FF01-387/11-25Emitter Part Number . . .FF02-447/60-25Dichroic Part Number . .FF409-Di02-25x36Full Set Prefix . . . . . . . . .DAPI-1160A-_ _ _

Exciter Part Number . . .FF01-377/50-25Emitter Part Number . . .FF02-447/60-25Dichroic Part Number . .FF409-Di02-25x36Full Set Prefix . . . . . . . . .DAPI-5060B-_ _ _

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CFP-2432A

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) filter set available for measuringCFP and its variants – no more compromising or exchanging filter setsfor different CFP experiments. Filters from this set are ideal for CFP-YFP FRET measurements when used in conjunction with filters fromour YFP-2427A filter set (see page 11 and page 14).

Exciter Part Number . . .FF01-438/24-25Emitter Part Number . . .FF01-483/32-25Dichroic Part Number . .FF458-Di01-25x36Full Set Prefix . . . . . . . . .CFP-2432A-_ _ _

Single-band Fluorescence Filter Sets – Made With ONLY Hard Coatings


DAPI-1160A – Highest Contrast

See page 6 for a list of primary fluorophores.Go to www.semrock.com for ASCII data and a complete fluorophore listing.

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New UV fluorescence filter set! Only Semrock offers true UV fluorescence filters with high transmission, edge steepness, and blockingthat rival high-performance visible filters. The filters in this set are idealfor imaging the intrinsic fluorescence from aromatic amino acids (like tryptophan) and other UV-excited fluorescent species. See page 9 for a Technical Note on UV Fluorescence.

Exciter Part Number . . . FF01-280/20-25Emitter Part Number. . . FF01-357/44-25-5.0Dichroic Part Number. . FF310-Di01-25x36Full Set Prefix . . . . . . . . . TRP-A-_ _ _

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DAPI-5060B – Highest Brightness

Highest Contrast DAPI set on the market. The preferred DAPI set for most applications, this filter set is optimized for achieving very low noise while maintaining excellent brightness, resulting in unsurpassed contrast. The signal-to-background ratio achieved with this DAPI set forimaging typical nuclear-stain samples is more than twice that of a leadingcompetitor’s.

Brightest DAPI set on the market. This filter set is recommendedfor those special cases in which it is critical to achieve the highest possible brightness for the shortest possible exposure times, or for very low fluorophore concentrations. For shorter exposure times, theset exhibits up to 5 times more brightness, but has lower contrast thanthe DAPI-1160A set.

Exciter Part Number . . .FF01-387/11-25Emitter Part Number . . .FF02-447/60-25Dichroic Part Number . .FF409-Di02-25x36Full Set Prefix . . . . . . . . .DAPI-1160A-_ _ _

Exciter Part Number . . .FF01-377/50-25Emitter Part Number . . .FF02-447/60-25Dichroic Part Number . .FF409-Di02-25x36Full Set Prefix . . . . . . . . .DAPI-5060B-_ _ _

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CFP-2432A

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) filter set available for measuringCFP and its variants – no more compromising or exchanging filter setsfor different CFP experiments. Filters from this set are ideal for CFP-YFP FRET measurements when used in conjunction with filters fromour YFP-2427A filter set (see page 11 and page 14).

Exciter Part Number . . .FF01-438/24-25Emitter Part Number . . .FF01-483/32-25Dichroic Part Number . .FF458-Di01-25x36Full Set Prefix . . . . . . . . .CFP-2432A-_ _ _

Single-band Fluorescence Filter Sets – Made With ONLY Hard Coatings


DAPI-1160A – Highest Contrast


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New UV fluorescence filter set! Only Semrock offers true UV fluorescence filters with high transmission, edge steepness, and blockingthat rival high-performance visible filters. The filters in this set are idealfor imaging the intrinsic fluorescence from aromatic amino acids (like tryptophan) and other UV-excited fluorescent species. See page 9 for a Technical Note on UV Fluorescence.

Exciter Part Number . . . FF01-280/20-25Emitter Part Number. . . FF01-357/44-25-5.0Dichroic Part Number. . FF310-Di01-25x36Full Set Prefix . . . . . . . . . TRP-A-_ _ _

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GFP-3035B

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) filter set available for measuringGFP and its variants – no more compromising or exchanging filter sets for different GFP experiments. Filters from this set are excellent for GFP-DsRed FRET measurements when used in conjunction with the FF01-593/40 filter. See page 14 for a description of FRET.

Exciter Part Number . . .FF01-472/30-25Emitter Part Number . . .FF01-520/35-25Dichroic Part Number . .FF495-Di02-25x36Full Set Prefix . . . . . . . . .GFP-3035B-_ _ _

Single-band Fluorescence Filter Sets – Highest Performance & Durability


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FITC-3540B

This set is optimized for the most popular green fluorophores like FITC(fluorescein isothiocyanate) and FAM. It provides the highest brightnessand signal-to-noise ratio. It is also ideal for similar fluorophores but withnarrower, more closely spaced excitation and emission peaks, like Bodipy.For FITC filter sets optimized for clinical applications, see page 23.

Exciter Part Number . . .FF01-482/35-25Emitter Part Number . . .FF01-536/40-25Dichroic Part Number . .FF506-Di02-25x36Full Set Prefix . . . . . . . . .FITC-3540B-_ _ _


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This four-filter set achieves high-performance ratiometric imaging of Ca2+ using Fura-2 as an indicator. The carefully optimized excitation filters provide the best balance of free and saturated Ca2+ signals whileminimizing the crosstalk and noise, even when using light sources of relatively limited ultraviolet output. See Technical Note on page 14.

FURA2-B

Exciter Part Number . . .FF01-340/26-25Exciter Part Number . . .FF01-387/11-25Emitter Part Number . . .FF01-510/84-25Dichroic Part Number . .FF409-Di02-25x36Full Set Prefix . . . . . . . . .FURA2-B-_ _ _

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YFP-2427A

This filter set is optimized to be simultaneously the brightest and thehighest signal-to-noise ratio (contrast) filter set available for measuringYFP and its variants – no more compromising or exchanging filter setsfor different YFP experiments. Filters from this set are ideal for CFP-YFPFRET measurements when used in conjunction with filters from ourCFP-2432A filter set (see page 10 and page 14).

Exciter Part Number . . . FF01-500/24-25Emitter Part Number. . . FF01-542/27-25Dichroic Part Number. . FF520-Di01-25x36Full Set Prefix . . . . . . . . . YFP-2427A-_ _ _

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Single-band Fluorescence Filter Sets – Highest Performance & Durability


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Cy5-4040A

This filter set is ideal for use with Cy5™ and other deep red fluorophores. It is optimized to provide the highest brightness, whilemaintaining a high signal-to-noise ratio.

Exciter Part Number . . .FF01-628/40-25Emitter Part Number . . .FF01-692/40-25Dichroic Part Number . .FF660-Di01-25x36Full Set Prefix . . . . . . . . .Cy5-4040A-_ _ _

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TXRED-4040B

This filter set is ideal for use with Texas Red® and other red fluorophores. It also works well for broader orange-to-red fluorophoreslike MitoTracker Red. It is optimized to provide the highest possiblebrightness, while maintaining a high signal-to-noise ratio.

Exciter Part Number . . .FF01-562/40-25Emitter Part Number . . .FF01-624/40-25Dichroic Part Number . .FF593-Di02-25x36Full Set Prefix . . . . . . . . .TXRED-4040B-_ _ _

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Cy3-4040B

This filter set is ideal for use with Cy3™ and a variety of other orangefluorophores (including DsRed fluorescent protein). It is optimized toprovide the highest brightness, while maintaining a very high signal-to-noise ratio. When co-labeling with YFP, GFP, or FITC, we recommendusing our TRITC-A set.

Exciter Part Number . . .FF01-531/40-25Emitter Part Number . . .FF01-593/40-25Dichroic Part Number . .FF562-Di02-25x36Full Set Prefix . . . . . . . . .Cy3-4040B-_ _ _

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This filter set is ideal for use with TRITC and a number of Rhodaminedyes, as well as other orange fluorophores.This improved set offers highbrightness, and yet with its narrow exciter filter, it also provides a highsignal-to-noise ratio and minimizes the bleedthrough in co-labeling appli-cations typical with shorter-wavelength yellow and green fluorescentmarkers such as YFP, GFP, and FITC.

Exciter Part Number . . . FF01-543/22-25Emitter Part Number. . . FF01-593/40-25Dichroic Part Number. . FF562-Di02-25x36Full Set Prefix . . . . . . . . . TRITC-A-_ _ _

TRITC-A


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Composite images produced from conventional filter sets (above left), which typically have significant pixel shift,are distorted, whereas BrightLine ZERO pixel shift filter sets(above right) yield precise multi-color images.

Allows you to create perfect multi-color composite images

Ideal for demanding applications like:

Co-localization fluorescence measurements

Fluorescence In Situ Hibridization (FISH)

Comparative Genomic Hybridization (CGH)

What is Pixel Shift?

Pixel shift results when a filter in an imaging path (the emitterand/or dichroic beamsplitter in a fluorescence microscope)with a non-zero wedge angle deviates the light rays so as tocause a shift of the image detected on a high-resolution CCDcamera. When two or more images of the same objectacquired using different filter sets are overlaid (in order tosimultaneously view fluorescence from multiple fluorophores),any significant non-zero filter wedge angle means that theimages will not be registered to identical pixels on the CCDcamera. Hence, images produced by different fluorophores willnot be accurately correlated or combined. On the other hand,BrightLine ZERO filter sets are manufactured, tested and certified to very tight tolerances so as to ensure accurateimage registration every time.

How do we do it?

Poor image registration, or pixel shift, results from the almostinevitable non-zero filter wedge angle. But low pixel shift is critical toobtain the best imaging performance when exchanging filters duringany measurements that involve multiple exposures.

Semrock’s advanced ion-beam sputtering (IBS) coating technology makes it possible for all BrightLine filters to beuniquely constructed from a single pieceof glass, with the permanent hard coat-ings applied directly to the outside.Thispatented[1] lower-loss and high-reliabilityconstruction inherently offers superiorimaging performance. BrightLine ZEROfilter substrates are further manufactured and tested to the most exacting tolerances for certified “zero-pixel-shift”performance.

With older soft-coated fluorescence filters, one is forced to use multiple substrates that are typically bonded together with adhesive, generally resulting in significant wedge angleand therefore pixel shift.To improve the imaging registration, extraprocessing steps, alignment steps, and/or compensating optics arerequired, resulting in added cost. By contrast, BrightLine ZERO filters areinherently manufacturable and thus very affordable.

Conventional Approach

Uncoated

Uncoated

AdhesiveSoft coatingAdhesiveSoft coating

BrightLine ZERO™

Hard Coating

Hard Coating

Glass


Semrock solves your image registration problem – just choose BrightLine ZERO fluorescence filter sets.All of the standardBrightLine single-band filter sets – from our DAPI sets through our Cy5 filter set – are optionally available with certified “zero-pixel-shift” performance (measured relative to other BrightLine ZERO sets).

BrightLine ZERO™ Fluorescence Filters – for Exact Image Registration


[1]U.S. Patent No. 6,809,859 and pending.

BrightLine ZERO fluorescence filter sets are very affordable – for a nominal amount more than the price of a BrightLine standard single-band set you can ensure exact image registration when making multi-color composite images. Not sure if you will need this?Keep in mind that BrightLine filters never burn out, and the ZERO option requires no calibration or special alignment – so why notfuture-proof your system? Our patented design is so manufacturable that BrightLine ZERO filters are in stock for quick delivery.Join your many colleagues and demand the “ZERO option” for certified image registration.

[1] Tested in popular microscope cubes.

For more information go to www.semrock.comto view our article from the August 2005 issue ofBioPhotonics International.

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Property Value Comment

Set-to-set Image Shift < ± 1 pixel Worst case image shift when interchanging BrightLine ZERO filter sets, as measuredrelative to the mean image position for a large sample of filter sets. Analysis assumescollimated light in a standard microscope with a 200 mm focal length tube lens and 6.7 micron pixel size.[1]

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Figure 2: CFP exciter and dichroic filters with an emitter filter (FF01-536/40) for quantitative measurement of the YFP (acceptor)emission.

Fluorescence Resonance Energy Transfer (FRET)

Fluorescence Resonance Energy Transfer (FRET) is a powerful technique for characterizing distance-dependent interactions on a molecular scale. FRET starts with the excitation of a donor fluorophore molecule by incident light within its absorption spectrum. Normally, the fluorophorewould decay radiatively with a characteristic emission spectrum; however, if another fluorophoremolecule (the acceptor) exists in close proximity to the donor and with its energy state character-ized by an absorption spectrum that overlaps the emission spectrum of the donor, then non-radia-tive energy transfer may occur between donor and acceptor. As an example, cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) support a strong FRET interaction. FRET is suited to measuring changes in distance on the order of the “Förster distance,” which is typically 20 to 90 Å.This length scale is far below the Rayleigh-criterion resolution limit of an optical microscope (typically 2500 Å for visible light at high numerical aperture), thus illustrating the powerof FRET for measuring extremely small distance interactions.

Application conditions for observing FRET are often characterized by extremely low fluorophore concentrations thus requiring detection of very faint emission levels. Semrock BrightLine®

fluorescence filters offer the highest possible transmission for maximizing the FRET emission signal,as well as carefully optimized deep blocking out of the transmission passbands, for maximum possi-ble signal-to-background ratios (highest contrast).

Figure 1 shows the CFP absorption and emission spectra along with the transmission spectra for a filter set optimized for measuring CFP (CFP-2432A). Figure 2 shows the same set but with a different emitter filter (FF01-536/40) and the emission spectrum for YFP. The first set is used toquantify the fluorescence from the donor alone, while the second set is used to quantify the FRETfluorescence from the acceptor.

Figure 1: CFP exciter, dichroic, andemitter filters (CFP-2432A set) forquantitative measurement of donoremission.

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Using Fura-2 to Track Ca 2+

The fluorophore Fura-2 has an absorption spectrum that varies markedly depending on the concentration of calcium (Ca2+) that is present near the fluorophore molecule. By measuring the ratio of intensities on digital images captured with two different excitation filters, the variation of calcium concentration at various locations can be tracked.

Compared to the highest performance competitive filter sets, the BrightLine FURA2-B set provides 4 times the brightness yet with substantially higher contrast, providing exceptionalhigh-speed imaging performance. Furthermore, the BrightLine set achieves superb balance of the saturated-to-free signal intensities, thus allowing the most accurate ratio calculations withminimal adjustment of camera settings.

Ca2+-free exciter

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BrightL

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The highest transmission and steepest edges for dazzling brightness – visually and digitally

Nearly perfect blocking for striking contrast – visually and digitally

ALL hard dielectric coatings, including blue and UV filters, for long-lasting no “burn-out” performance

Only Semrock manufactures multiband fluorescence filters with passband,edge steepness, and blocking performance that rival the best single-bandfilters, and all with the superior, no “burn-out” durability of hard coatings.In fact, every filter in every BrightLine filter set, including these multibandsets, is made with the same, durable hard-coating technology (see page 3).So you will always see…


Multiband Fluorescence Filter Sets (pages 15-19)

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Graph above shows typical measured transmission of the FF01-425/527/685-25 filter

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Typical measured spectra of the DA/FI/TX “Full Multiband” filter set

This full line of filter sets is optimized forimaging the most popular blue, green, andred fluorophores. The “Full Multiband” setallows simultaneous direct viewing or imag-ing with a color camera. The “Pinkel” set,with single-band exciters, and the “Sedat”set, with single-band exciters and emitters,are ideal for three-color high-speed imagingusing a low-noise monochrome camera anda filter wheel or wheels.

DAPI/FITC/Texas Red in “Full Multiband,” “Pinkel” and “Sedat” Filter Sets

Filters and Part Numbers for the “Pinkel”configuration:Exciter 1 Part No. . .FF01-387/11-25Exciter 2 Part No. . .FF01-494/20-25Exciter 3 Part No. . .FF01-575/25-25Emitter Part No. . . .FF01-457/530/628-25Dichroic Part No. . .FF436/514/604-Di01-25x36Full Set Prefix . . . . .DA/FI/TX-3X-A-___

Typical measured spectra of the DA/FI/TX “Pinkel” filter set

Filters and Part Numbers for the “Sedat”configuration:Exciter 1 Part No. . .FF01-387/11-25Exciter 2 Part No. . .FF01-494/20-25Exciter 3 Part No. . .FF01-575/25-25Emitter 1 Part No. . .FF02-447/60-25Emitter 2 Part No. . .FF01-531/22-25Emitter 3 Part No. . .FF01-624/40-25Dichroic Part No. . .FF436/514/604-Di01-25x36Full Set Prefix . . . . .DA/FI/TX-3X3M-A-___

Filters and Part Numbers for the “Full Multiband” configuration:Exciter Part No. . . . .FF01-407/494/576-25Emitter Part No. . . . .FF01-457/530/628-25Dichroic Part No. . .FF436/514/604-Di01-25x36Full Set Prefix . . . . . .DA/FI/TX-B-_ _ _

Semrock’s award-winning multiband filter sets are uniquely optimized to provide brilliant colorsand a very black background. In this example, the relative signal, noise, and signal-to-noise ratioachieved by a BrightLine DA/FI/TX-B filter set wascompared side-by-side with the performance of thepremium Full Multiband set of a leading competitor.The BrightLine filters are both 50% brighter andprovide a stunning 2.4 times higher contrast.

SNR

Noise

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Go to www.semrock.com for ASCII data and a complete fluorophore listing.


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Multiband Sets for Fluorescent Proteins

All BrightLine single-band bandpass filters in “Pinkel” and “Sedat” sets, including for Leica microscopes, come with standard 25 mm (32 mm optional) exciters and 25 mm emitters, and are packaged separately for convenient mountingin standard filter wheels. For part numbers for Leica microscopes, see www.semrock.com.

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Typical measured spectra of the CFP/YFP “Full Multiband” filter set

This full line of filter sets is optimized forimaging samples dual-labeled with cyan andyellow fluorescent proteins (CFP and YFP).The “Full Multiband” set allows simultane-ous direct viewing or imaging with a colorcamera. The “Pinkel” set, with single-bandexciters, and the “Sedat” set, with single-band exciters and emitters, are ideal fortwo-color high-speed imaging using a low-noise monochrome camera and a filterwheel or wheels.

CFP/YFP “Full Multiband,” “Pinkel” and “Sedat” Filter Sets

Filters and Part Numbers for the “Pinkel”configuration:Exciter 1 Part Number . . .FF01-427/10-25Exciter 2 Part Number . . .FF01-504/12-25Emitter Part Number . . . .FF01-464/547-25Dichroic Part Number . . .FF440/520-Di01-25x36Full Set Prefix . . . . . . . . . . .CFP/YFP-2X-A-___

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Typical measured spectra of the CFP/YFP “Pinkel” filter set

Filters and Part Numbers for the “Sedat”configuration:Exciter 1 Part Number . .FF01-427/10-25Exciter 2 Part Number . .FF01-504/12-25Emitter 1 Part Number . .FF01-472/30-25Emitter 2 Part Number . .FF01-542/27-25Dichroic Part Number . .FF440/520-Di01-25x36Full Set Prefix . . . . . . . . . .CFP/YFP-2X2M-A-___

Filters and Part Numbers for the “Full Multiband” configuration:Exciter Part Number . . . . .FF01-416/501-25Emitter Part Number . . . .FF01-464/547-25Dichroic Part Number . . .FF440/520-Di01-25x36Full Set Prefix . . . . . . . . . . .CFP/YFP-A-___

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Filters and Part Numbers for the “Pinkel” configuration:Exciter 1 Part Number . . . . .FF01-427/10-25Exciter 2 Part Number . . . . .FF01-504/12-25Exciter 3 Part Number . . . . .FF01-589/15-25Emitter Part Number . . . . . .FF01-464/542/639-25Dichroic Part Number . . . . .FF444/521/608-Di01-25x36Full Set Prefix . . . . . . . . . . . .CFP/YFP/HcRed-3X-A-___

Filters and Part Numbers for the “Sedat” configuration:Exciter 1 Part Number . . . . .FF01-427/10-25Exciter 2 Part Number . . . . .FF01-504/12-25Exciter 3 Part Number . . . . .FF01-589/15-25Emitter 1 Part Number . . . . .FF01-472/30-25Emitter 2 Part Number . . . . .FF01-542/27-25Emitter 3 Part Number . . . . .FF01-632/22-25Dichroic Part Number . . . . .FF444/521/608-Di01-25x36Full Set Prefix . . . . . . . . . . . .CFP/YFP/HcRed-3X3M-A-___

Typical measured spectra of the CFP/YFP/HcRed “Pinkel” filter set

These filter sets are ideal for imaging samples triple-labeled withcyan and yellow fluorescent pro-teins (CFP and YFP) and HcRed withhigh brightness, extremely lowcrosstalk, and superb signal-to-noise ratio. They are also excellentfor samples triple-labeled with CFP,YFP, and an orange or red dye, likeTexas Red. The “Pinkel” configura-tion has three single-band exciters,a triple-band beamsplitter and atriple-band emitter. The “Sedat”configuration has single-bandexciters and emitters with a triple-band beamsplitter. The single-bandfilters are meant to go in a filterwheel or slider.

CFP/YFP/HcRed “Pinkel” and “Sedat” Filter Sets

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Go to www.semrock.comfor ASCII data and a

complete fluorophore listing.

This composite image was created from separate monochromeimages captured using a Pinkel filter set. The sample is a Hela cell expressing SECFP, Venus and mRFP targeted to theendoplasmic reticulum, the mitochondria and the nucleus,respectively. The wide-field image was taken on a NikonTE2000E inverted microscope with a 60X, PlanApo, 1.4-NA,oil-immersion objective, and a cooled monochrome CCD camera (Orca-ER, Hamamatsu Photonics) using a BrightLineCFP/YFP/HcRed-3X-A filter set.

Courtesy of Takeharu Nagai and Kenta Saito, Laboratory forNanosystems Physiology, REIS, Hokkaido University.

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Multiband Sets for Fluorescent Proteins

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These filter sets are ideal for imaging samples dual-labeled with GFP and DsRed with high brightness, extemely low crosstalk, and superb signal-to-noise ratio. They are also ideal for samples dual-labeled with FITC and TRITC, or GFP and Cy3, among other fluorophore combinations. The “Pinkel”set has single-band exciters with a dual-band beamsplitter and dual-band emitter. The “Sedat” set has single-band exciters and emitters with a dual-band beamsplitter. The single-band filters are meant to go in a filter wheel or wheels.

Exciter 1 Part Number . . . . .FF01-470/22-25Exciter 2 Part Number . . . . .FF01-556/20-25Emitter Part Number . . . . . .FF01-512/630-25Dichroic Part Number . . . . .FF493/574-Di01-25x36Full Set Prefix . . . . . . . . . . . .GFP/DsRed-2X-A-___

Exciter 1 Part Number . . . . .FF01-470/22-25Exciter 2 Part Number . . . . .FF01-556/20-25Emitter 1 Part Number . . . . .FF01-514/30-25Emitter 2 Part Number . . . . .FF01-617/73-25Dichroic Part Number . . . . .FF493/574-Di01-25x36Full Set Prefix . . . . . . . . . . . .GFP/DsRed-2X2M-B-___

Typical measured spectra of the GFP/DsRed “Pinkel” filter set

Typical measured spectra of the GFP/DsRed “Sedat” filter set

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GFP/DsRed “Pinkel” and “Sedat” Filter Sets

All BrightLine single-band bandpass filters in “Pinkel” and “Sedat” sets, including for Leica microscopes, come with standard 25 mm(32 mm optional) exciters and 25 mm emitters, and are packaged separately for convenient mounting in standard filter wheels.For part numbers for Leica microscopes, see www.semrock.com.

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This filter set is ideal for imaging samples that are dual-labeled withgreen fluorescent protein (GFP) and HcRed with excellent brightnessand minimal crosstalk. It is also excellent for samples dual-labeled with FITC and Texas Red, for example.The green channel is brighter and the red channel is substantially red-shifted compared to theGFP/DsRed-2X-A filter set.

Exciter 1 Part Number . . . . .FF01-474/23-25Exciter 2 Part Number . . . . .FF01-585/29-25Emitter Part Number . . . . . .FF01-527/645-25Dichroic Part Number . . . . .FF495/605-Di01-25x36Full Set Prefix . . . . . . . . . . . .GFP/HcRed-2X-A-___

GFP/HcRed “Pinkel” Filter Set

This filter set is ideal for imaging samples that are triple-labeled withblue, green, and red fluorescent proteins (BFP, GFP, and HcRed in particular). It is also excellent for samples with a DAPI (or Hoechst)stain in the blue channel, and other green or red fluorophores like FITCor Texas Red in the green and red channels, respectively. The red band is very wide to allow maximum brightness in this channel.

Exciter 1 Part Number . . . . .FF01-370/36-25Exciter 2 Part Number . . . . .FF01-474/23-25Exciter 3 Part Number . . . . .FF01-585/29-25Emitter Part Number . . . . . .FF01-425/527/685-25Dichroic Part Number . . . . .FF395/495/610-Di01-25x36Full Set Prefix . . . . . . . . . . . .BFP/GFP/HcRed-3X-A-___

BFP/GFP/HcRed “Pinkel” Filter Set

Typical measured spectra of the GFP/HcRed “Pinkel” filter set

Typical measured spectra of the BFP/GFP/HcRed “Pinkel” filter set

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Quad-band Filter Set – the Flagship of Multiband Sets!

Independently tested: 4 times brighter... and twice the contrast!

Leading Competitor’s Quad-band “Pinkel” Filter Set Semrock BrightLine Quad-band “Pinkel” Filter Set

Revolutionary performance

Most versatile multiband sets on the market

All no “burn-out” exciters

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Exciter 1 Part Number . . . . .FF01-387/11-25Exciter 2 Part Number . . . . .FF01-485/20-25Exciter 3 Part Number . . . . .FF01-560/25-25Exciter 4 Part Number . . . . .FF01-650/13-25Emitter 1 Part Number . . . . .FF01-440/40-25Emitter 2 Part Number . . . . .FF01-525/30-25Emitter 3 Part Number . . . . .FF01-607/36-25Emitter 4 Part Number . . . . .FF01-684/24-25Dichroic Part Number . . . . .FF410/504/582/669-Di01-25x36Full Set Prefix . . . . . . . . . . . .DA/FI/TR/Cy5-4X4M-B-___

Typical measured spectra for the Quad-band DA/FI/TR/Cy5 “Pinkel” filter set

Comparisons done under identical imaging conditions using an Olympus BX61WI microscope outfitted with DSU spinning-disk confocal unit and Hamamatsu ORCA-ER monochromeCCD camera. Sample of Rat Kidney Mesangial Cells courtesy of Mike Davidson, Molecular Expressions™, using: Hoechst 33258, Alexa Fluor 488 – Phalloidin, MitoTracker Red CMXRos,and Vimentin (Ms) – Cy5. Semrock DA/FI/TR/Cy5-4X-A filter set.

Quad-band “Pinkel” and “Sedat” Filter Sets

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Exciter 1 Part Number . . . . .FF01-387/11-25Exciter 2 Part Number . . . . .FF01-485/20-25Exciter 3 Part Number . . . . .FF01-560/25-25Exciter 4 Part Number . . . . .FF01-650/13-25Emitter Part Number . . . . . .FF01-440/521/607/700-25Dichroic Part Number . . . . .FF410/504/582/669-Di01-25x36Full Set Prefix . . . . . . . . . . . .DA/FI/TR/Cy5-4X-A-___

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Typical measured spectra for the Quad-band DA/FI/TR/Cy5 “Sedat” filter set

Semrock's state-of-the-art DAPI-FITC-TRITC-Cy5 filter sets are ideal for creating the brightest 4-color composite images with superior contrast. The six-filter “Pinkel” set includes exceptionally efficient quad-band beamsplitter and quad-band emission filtersmated with four single-band exciters, all of which utilize Semrock’s no “burn-out” hard-coating technology. With its exceptionallylow crosstalk, performance rivals the fidelity achieved by single-band sets. The nine-filter “Sedat” set provides the ultimate in high-speed, low-crosstalk imaging performance, with all no “burn-out” hard-coated filters – including the blue and UV filters.

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“Full Multiband” Configuration(Multiband exciter, multiband emitter,

& multiband dichroic)


“Pinkel” Configuration(Multiband emitter, multiband

dichroic, & single-band exciters)

“Sedat” Configuration(Multiband dichroic, single-band exciters,

& single-band emitters)

Multiband Filter Set Terminology

The ability to label multiple, distinct objects of interest in a singlesample greatly enhances the power of fluorescence imaging. Fora long time the only way to achieve high-quality images of suchsamples was to take multiple photographs while switching wholesingle-band filter cubes between photographs, and then latercombine these photographs electronically. Limitations to thisapproach included “pixel shift” among the multiple, single-colorimages and the speed with which a complete multi-color imagecould be captured. Semrock solved the problem of “pixel shift”with its BrightLine ZERO™ technology (see page 13 for a completeexplanation), and the single-band filter cube approach remains thebest technique for achieving images with the highest contrast andlowest bleedthrough possible. But with increasing demand forhigh-speed imaging, especially for live-cell real-time analysis usingfluorescent protein labels, there is a need for an alternative tothe single-band filter cube approach without sacrificing too muchimage fidelity. Now Semrock’s recent advances in multiband optical filter technology have brought simultaneous multi-colorimaging to a new level.

There are three types of multiband filter sets for simultaneous multi-color imaging. The “full-multiband” configuration uses all multiband filters – exciter, emitter, and dichroic beamsplitter – and is ideal fordirect visualization, such as when locating areas of interest on a sample. This approach is quick and easy to implement and is compatible with all standard fluorescence microscopes, though itrequires a color camera for electronic imaging and cannot eliminate fluorophore bleedthrough. The “Pinkel” configuration uses single-band exciters in a filter wheel with multiband emitter and dichroicfilters, and offers an economical way to achieve very high-speed,high-contrast, simultaneous multi-color imaging. This approach isbased on a monochrome CCD camera, which is less expensive andoffers better noise performance than color cameras. Whilebleedthrough is reduced relative to the full-multiband approach,some bleedthrough is still possible since all emission bands areimaged simultaneously. The “Sedat” configuration uses single-bandexciters and single-band emitters in synchronized filter wheels, witha multiband dichroic beamsplitter. This approach provides the bestimage fidelity for high-speed simultaneous multi-color imaging,though it requires a larger investment in system hardware. Seewww.semrock.com for our 2006 BioPhotonics International article.

“Full Multiband” ImageMulti-color image captured with a color CCD camera

“Pinkel” and “Sedat” Composite ImageSingle-color images are combined electronically to produce one high-fidelity, multi-color image.


T-Cell and AntigenPresenting Cell (APC)conjugates demonstrating animmunologic synapse. Samplescourtesy Beth Graf and Dr. Jim Miller at the Universityof Rochester Medical Center.

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These BrightLine multiphoton ultrahigh-performance fluorescence filters serve a fullrange of applications with just two sets of filters, thus conveniently accommodatingthe wide range of fluorescent dyes that are the essential tools of the modernresearcher. The transmission bands of the emitters are so wide that they appearclear at normal incidence, and the reflection bands of the dichroics are so wide thatthey look like mirrors (at 45° incidence).

Multiphoton Filters – Our Hard Coatings are Laser Friendly

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These filters provide exceptional OD > 8 blocking for detectingthe lowest fluorophore concentrations, with transmission even downto near-UV wavelengths.

Near-UV & Visible Filters


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These filters provide excellent detection of fluorescence throughoutthe full visible wavelength range, including red fluorophores like Cy5™.

Full Visible Filters

Common SpecificationsProperty Emitter Dichroic Comment

Passband Transmission Guaranteed > 90% > 90% Averaged over passbandTypical > 95% > 95%

Dichroic Reflection N/A > 98% Averaged over exciter band(s)Autofluorescence Ultra-low Ultra-low Fused silica substrateBlocking Emitter filters have exceptional blocking over the Ti:Sapphire laser range as needed to achieve superb

signal-to-noise ratios even when using an extended-response PMT or a CCD camera or other silicon-based detector; see www.semrock.com for detailed specifications.

Pulse Dispersion Dichroic beamsplitters are suitable for use with 100 femtosecond gaussian laser pulses.Emitter Orientation The emitter orientation does not affect its performance; therefore there is no arrow on the ring to denote

a preferred orientation.Dichroic Orientation "Reflective coating side" should face toward detector and sample; see diagram on page 8Microscope Compatibility These filters fit most standard-sized microscope cubes from Nikon, Olympus, and Zeiss and may also be

mounted in optical-bench mounts. Contact Semrock for special filter sizes.

All other specifications are the same as standard BrightLine specifications on page 8.

Laser Blocking Emission Filter – FF01-680/SP-25 Transmission Range (Tavg > 90%) . . . . . . . . . . . . . .350-650 nmLaser Blocking Range . . . . . . . . . . . . . . . . . . . . . . .OD > 8: 680-1040 nm

OD > 6: 1040-1080 nm

Dichroic Beamsplitter – FF665-Di01-25x36Transmitted Laser Wavelengths (Tavg > 90%) . . .680-1100 nmReflection Bands (Ravg > 98%) . . . . . . . . . . . . . . . .350-650 nm

Laser Blocking Emission Filter – FF01-750/SP-25Transmission Range (Tavg > 90%) . . . . . . . . . . . . . .380-720 nmLaser Blocking Range . . . . . . . . . . . . . . . . . . . . . . .OD > 6: 750-1100 nm

Dichroic Beamsplitter – FF735-Di01-25x36Transmitted Laser Wavelengths (Tavg > 90%) . . .750-1100 nmReflection Bands (Ravg > 98%) . . . . . . . . . . . . . . . .350-720 nm

Emitter Short Wave Pass . . . .FF01-680/SP-25Dichroic Long Wave Pass . . . .FF665-Di01-25x36

Emitter Short Wave Pass . . . .FF01-750/SP-25Dichroic Long Wave Pass . . . .FF735-Di01-25x36

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Multiphoton filters

Multiphoton fluorescence microscopy is similar to traditional fluorescence microscopyin that fluorescent molecules that tag targets of interest in a cell or other specimen areexcited and subsequently emit fluorescent photons that are collected to form an image.However, in a two-photon microscope, for example, the molecule is not excited with asingle photon, as it is in traditional fluorescence, but instead two photons – each withtwice the wavelength – are absorbed simultaneously to excite the molecule (Figure 1).

As shown in Figure 2, a typical system is com-prised of an excitation laser, scanning and imag-ing optics, a sensitive detector (usually a photo-multiplier tube), and optical filters for separatingthe fluorescence from the laser (dichroic beam-splitter) and blocking the laser light from reach-ing the detector (emission filter).

The advantages offered by multiphoton imagingsystems include: true three-dimensional imag-ing, or optical sectioning, like confocalmicroscopy; the ability to image deep inside oflive tissue; elimination of out-of-plane fluores-cence; and reduction of photobleaching awayfrom the focal plane to increase sample longevi-ty. In addition, with this method it is possible toimage fluorescent dyes with very short Stokesshifts and/or very low efficiencies, and eveninherently fluorescent molecules native to thesample or tissue. Disadvantages of multiphotonimaging include the need for a high-peak-power,

pulsed laser, such as a mode-locked Ti:Sapphire laser, and, until now, the lack of high-per-formance optical filters that provide sufficient throughput across the whole emissionrange of interest and sufficient blocking across the full laser tuning range (Figure 3).

Now Semrock has brought enhanced performance to multiphoton users by introducingoptical filters with ultra-high transmission in the passbands, very steep transitions, and guaranteed deep blocking everywhere it is needed. Given how much investment is typically required for the excitation laser and other complex elements of multiphotonimaging systems, these new filters represent a simple and inexpensive upgrade to substantially boost system performance.

The new BrightLine emission filters provide crystal-clear transmission from thenear-UV to the near-IR (Figure 4). In fact, by eye the filters look as clear as windowglass (Figure 5), in contrast to the brownish tint of traditional filters. At the sametime, the dichroic beamsplitters are designed to reflect the precious fluorescencesignal with exceptionally high efficiency. The emission filters also provide deepblocking across the Ti:Sapphire laser tuning range, which is critical to achieving highsignal-to-noise ratio and measurement sensitivity.

Sometimes it is desirable torestrict the spectral band of fluorescence emission detectedat any given time, especially whenmultiple fluorophores are used tolabel different targets in a sample.Narrower bandpass emission filters are ideal for this purpose,and Semrock provides a widevariety of these bandpass filters(see page 24) that may be com-bined with a multiphoton emitterwith almost no loss of fluorescencesignal.


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3-Photon Fluorescence

Figure 1: Two-color in-vivo two-photon imaging from theexposed mouse cortex. NADH fluorescence (red) and sul-forhodamine-labeled astrocytes (green) taken usingBrightLine FF01-680/SP emitter and FF665-Di01 dichroic.Image courtesy of Karl A. Kasischke and Nikhil Mutyal, Dept.of Neurosurgery, University of Rochester Medical Center.

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Figure 3: Multiphoton microscopes require control oflight over a very wide spectrum: from the near-UV all theway through the near-IR.

Figure 5: BrightLine multiphoton filters are crystal clear!

Figure 2: Typical configuration of a multiphoton fluorescence microscope.

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See www.semrock.com for our recent BioPhotonics International article on multiphoton microscopy.



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First, BrightLine fluorescence filters established the standard in optical filters for research microscopy. Now, after years of continuous development, Semrock has made it possible to adapt the advanced hard-coating Ion Beam Sputtering technology usedfor BrightLine research filters to the more cost-conscious clinical market. The new BrightLine Clinical fluorescence filter sets arecarefully cost-reduced, and yet their optical performance exceeds that of even the premium traditional soft-coated fluorescencefilters, while retaining all the proven durability of BrightLine research-grade filters. These all-new patent pending BrightLine Clinicalhard-coated fluorescence filter sets are poised to set the standard in affordable performance for clinical laboratories!

BrightLine Clinical™ Fluorescence Filter Sets – Stunning ValueA Dramatic Advance in Clinical Microscopy!

Proven reliability and durability – for identical measurements year after year

Dazzling brightness and stunning contrast – for faster, surer diagnoses

Fully cost-competitive – plus no “burn out” so you never have to replace them

Take your laboratory to the next level – ask your microscope dealer to equip your microscope with Semrock’s amazing BrightLine Clinicalfluorescence filters.


Color photograph of toxoplasma gondii sample tagged with FITCand Evans Blue and taken using a FITC-LP01-Clinical filter set.

Color photograph of fungal yeast cell sample tagged withCalcofluor White and taken using a CFW-LP01-Clinical filter set.

“Very impressed.”

“Spectacular!”

“Dark background makes thefluorescence look more intense.”

“I like this better than the usual milky-white background.”

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Competitive FiltersBrightLine Clinical Filters

Comparison of the performance of FITC-LP01-Clinical filter set tocompetitive filter sets in widespread use today. Tests were per-formed at three different hospital clinical laboratories with at leasttwo experts from each laboratory. Testers were asked to rank theperformance on a scale of 1 to 5, with 5 representing the bestpossible performance.

Comments from independent clinical laboratory personnel

Hard-coated performance at a soft-coated price™

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These filter sets are optimized for the popular Calcofluor White fluorochrome used in a wide variety of mycological and fungal staining tests and kits.Samples will exhibit amazingly low background and high contrast for easy and accurate identification in test after test. The long-pass (LP01) set offershigher brightness and a dim “blue-white” background to provide clear color contrast (in addition to light-level contrast). The bandpass (BP01) setoffers the lowest background and highest light-level contrast in exchange for some brightness.

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BrightLine Clinical™ Fluorescence Filter Sets (continued)


Bandpass Calcofluor White Filter Set

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These filter sets are optimized for all popular tests based on blue excitation and green fluorescence. These include both direct (DFA) and indirect(IFA) immunofluorescent tests utilizing the FITC fluorochrome. Samples will exhibit amazing brightness and superior contrast for easy and accurateidentification in test after test. The long-pass (LP01) set offers higher brightness and the ability to observe longer-wavelength (e.g. yellow, orange, andred) fluorescence from counter-stains such as Rhodamine and Evans Blue. The bandpass (BP01) set is ideal for seeing only green fluorescence andoffers higher contrast in exchange for some brightness.

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Long Pass FITC Filter Set

FITC-LP01-Clinical

Bandpass FITC Filter Set

For full sets mounted in cubes, or filter holders, simply append the cube part number on page 7. For example, CFW-LP01-Clinical-ZHE is a long-pass CalcofluorWhite set mounted in a Zeiss ZHE cube.


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Part Number Primary Emitter Emitter Center Emitter Exciter Center ExciterPrefix Fluorophores Type or Edge Wavelength Bandwidth Wavelength Bandwidth

CFW-LP01-Clinical- Calcofluor White, DAPI Long Pass 416 nm N/A 387 nm 11 nm

CFW-BP01-Clinical- Calcofluor White, DAPI Bandpass 442 nm 46 nm 387 nm 11 nm

FITC-LP01-Clinical- FITC, Acridine Orange Long Pass 515 nm N/A 475 nm 28 nm

FITC-BP01-Clinical- FITC, Acridine Orange Bandpass 529 nm 28 nm 475 nm 28 nm


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Semrock stocks a wide selection of individual fluorscence bandpass filters that have been optimized for use in a variety of fluorescence instruments. For complete data describing these filters and the most up-to-date listing of available filters please visitwww.semrock.com.

These single-band bandpass filters all utilize Semrock’s patented single-substrate construction.The substrate is high-optical-quality,low-autofluorescence glass. Unless otherwise noted, all filters are housed in a standard 25 mm round black-anodized aluminum ringwith thickness as indicated, and a clear aperture of at least 21 mm. Parts denoted with a “-D” are unmounted. Parts with a “/LP” inthe part number are long-pass filters and parts with a “/SP” are short-pass filters.

Single-band Bandpass Filters

(continued)

Blocking varies for different filters. For graphs, ASCII data, and blocking information, go to www.semrock.com.

[1] Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.

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Emission Center Average Transmission SizeColor Filter Wavelength & Bandwidth [1] (Diameter x Thickness)

FF01-280/20-25 280 nm > 65% over 20 nm 25 mm x 5 mm

FF01-300/LP-25 306 nm (edge) > 85% 308-420 nm 25 mm x 5 mm

FF01-310/SP-25 293 nm (edge) > 70% 270-290 nm 25 mm x 3.5 mm


FF01-341/LP-25 347 nm (edge) > 90% 350-500 nm 25 mm x 3.5 mm

FF01-355/40-25 355 nm > 80% over 40 nm 25 mm x 3.5 mm














FF02 -447/60-25 447 nm > 90% over 60 nm 25 mm x 3.5 mm



















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Single-band Bandpass Filters (continued)


(continued)[1] Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.

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FF01-513/17-25 513 nm > 90% over 17 nm 25 mm x 3.5 nm


FF01-514/LP-25 527 nm (edge) > 90% 530-800 nm 25 mm x 5 mm




FF01-525/30-25 525 nm > 90 % over 30 nm 25 mm x 3.5 mm





FF01-530/200-25-D 530 nm > 90% over 200 nm (UV/IR blocking filter) 25 mm x 3 mm (no cell)





FF01-536/40-25 536 nm >90% over 40 nm 25 mm x 3.5 mm

FF01-537/26-25 537 nm >90% over 26 nm 25 mm x 5 mm




FF01-543/22-25 543 nm >90% over 22 nm 25 mm x 5 mm

FF01-550/220-25 550 nm > 90% over 220 nm (UV/IR blocking filter) 25 mm x 5 mm

FF01-554/211-25 554 nm > 90% over 211 nm (UV/IR blocking filter) 25 mm x 5 mm










FF01-580/60-25-D 580 nm > 80% over 60 nm 25 mm x 4 mm (no cell)





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Single-band Bandpass Filters (continued)


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[1] Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.



























FF01-680/SP-25 654 nm (edge) See Multiphoton Filters, page 20




FF01-697/75-25-D 697 nm > 90% over 75 nm 25 mm x 4 mm (no cell)





FF01-750/SP-25 727 nm (edge) See Multiphoton Filters, page 20









Call us: +49 (0)8153 405-02�




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Semrock offers a selection of individual multiband fluorescence bandpass filters that have been optimized for use in a variety of fluorescence instruments. These filters all utilize Semrock’s patented single-substrate construction.Thesubstrate is high-optical-quality, low-autofluorescence glass. All filters are housed in a standard 25 mm round black-anodized aluminum ring with thickness as indicated, and a clear aperture of at least 21 mm.

Multiband Bandpass Filters

State-of-the-art Multiband Filters

Semrock’s unique ion-beam sputtered filter technology allows us to manufacture the highest-performance multiband filters on themarket. Note the extremely high transmission, steep and well-defined edges, and outstanding blocking between the passbands ofthis triple-band filter (FF01-464/542/639-25) – just one example.

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Emission Center Average Transmission SizeColor Filter Wavelength & Bandwidth (Diameter x Thickness)

Dual-band Filters

FF01-416/501-25 416 nm > 90% over 25 nm 25 mm x 5 mm501 nm > 90% over 18 nm


FF01-464/547-25 464 nm > 90% over 23 nm 25 mm x 3.5 mm547 nm > 90% over 31 nm








Triple-band Filters

FF01-407/494/576-25 407 nm > 80% over 14 nm 25 mm x 5 mm494 nm > 85% over 20 nm576 nm > 85% over 20 nm

FF01-425/527/685-25 425 nm > 90% over 35 nm 25 mm x 3.5 mm527 nm > 90% over 42 nm685 nm > 90% over 130 nm




Quadruple-band Filters

FF01-440/521/607/700-25 440 nm > 90% over 40 nm 25 mm x 3.5 mm521 nm > 90% over 21 nm607 nm > 90% over 34 nm700 nm > 90% over 45 nm

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Single-edge Dichroic Beamsplitters (polarization-insensitive; for use at 45°)

Semrock offers a selection of polarization-insensitive dichroic beamsplitters for 45° angle-of-incidence that exhibit steep edges withvery high and flat reflection and transmission bands. More complete reflection and transmission mean less stray light for lowerbackground and improved signal-to-noise ratio. These filters are optimized for fluorescence microscopes and instrumentation, andmay also be used for a variety of other applications that require beam combining and separation based on wavelength. All Semrockfilters are made with our reliable hard-coating technology. Our dichroics utilize high-optical-quality, ultralow-autofluorescence glasssubstrates. Go to www.semrock.com for ASCII data and spectra graphs.

Dichroic Beamsplitters – ONLY Hard Coatings


[1] Wavelength ranges over which average reflection and transmission are guaranteed to be above 98% and 90%, respectively.

The examples below show high-peformance single-edge dichroic beamsplitters.They are long-wave-pass filters used at 45 degrees and are ideal for epi-fluorescenceillumination applications, in which the beamsplitter reflects the excitation light andtransmits the fluorescence.The new FF310-Di01 is an ultraviolet (UV) filter that provides very high reflection of light in the 250-300 nm range.


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NominalEmission Edge Reflection TransmissionColor Filter Wavelength Band [1] Band [1] Special Features Size

FF310-Di01-25x36 310 nm 255-295 nm 315-600 nm 25.2 mm x 35.6 mm x 1.1 mm




FF506-Di02-25x36 506 nm 446-500 nm 513-725 nm Ideal for separating CFP & YFP 25.2 mm x 35.6 mm x 1.1 mmemission paths in a 2-detector system


FF555-Di02-25x36 555 nm 493-548 nm 562-745 nm Ideal for separating GFP & RFP 25.2 mm x 35.6 mm x 1.1 mmemission paths in a 2-detector system





FF665-Di01-25x36 665 nm See Multiphoton Filters, page 20

FF669-Di01-25x36x3.0 669 nm 350-660 nm 677-800 nm 25.2 mm x 35.6 mm x 3.0 mm

FF735-Di01-25x36 735 nm See Multiphoton Filters, page 20


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Triple-edge Dichroic Beamsplitters (polarization-insensitive; for use at 45°)

Dichroic Beamsplitters (continued)

Dual-edge Dichroic Beamsplitters (polarization-insensitive; for use at 45°)



NominalEmission Edge Reflection TransmissionColor Filter Wavelength Band [1] Band [1] Special Features Size

FF440/520-Di01-25x36 440 nm 415-432 nm 449-483 nm 25.2 mm x 35.6 mm x 1.1 mm520 nm 493-511 nm 530-569 nm





561-700 nmFF497/661-Di01-25x36 497 nm 486-490 nm 420-471 nm 25.2 mm x 35.6 mm x 1.1 mm

661 nm 646-648 nm 505-626 nm668-750 nm



646 nm 632-634 nm 505-613 nm653-750 nm


FF545/650-Di01-25x36 545 nm 532.0 nm 554-613 nm 25.2 mm x 35.6 mm x 1.1 mm 650 nm 632.8 nm 658-742 nm



646 nm 632-634 nm 561-613 nm653-750 nm



644 nm 632-634 nm 587-613 nm653-750 nm

Designed for reflecting laserwavelengths 543 nm and 633 nm


Designed for blocking laserwavelengths 488 nm and 543 nm




Designed for dual-laser excitation(532 and 633 nm) of Cy3 and Cy5


Emission Nominal Edge Reflection TransmissionColor Filter Wavelength Band [1] Band [1] Size

FF395/495/610-Di01-25x36 395 nm 354-385 nm 403-446 nm 25.2 mm x 35.6 mm x 1.1 mm495 nm 465-483 nm 502-552 nm610 nm 570-596 nm 620-750 nm

FF436/514/604-Di01-25x36 436 nm 394-414 nm 446-468 nm 25.2 mm x 35.6 mm x 1.1 mm 514 nm 484-504 nm 520-540 nm604 nm 566-586 nm 614-642 nm

FF444/521/608-Di01-25x36 444 nm 420-430 nm 451-480 nm 25.2 mm x 35.6 mm x 1.1 mm521 nm 496-510 nm 530-561 nm608 nm 579-596 nm 618-664 nm

FF462/522/607-Di01- 462 nm 430-453 nm 471-489 nm 25.2 mm x 35.6 mm x 3.5 mm25x36x3.5 522 nm 507-512 nm 532-560 nm

607 nm 580-595 nm 619-750 nmFF494/540/650-Di01- 494 nm 488 nm 500-519 nm 25.2 mm x 35.6 mm x 3.5 mm

25x36x3.5 (s polarization only) (NBK7 substrate)540 nm 532 nm 545-610 nm650 nm 633-642 nm 655-700 nm


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The examples at the rightshow two high performancemultiband beamsplitters.The Di01-T488/568/638transmits three differentlaser lines and reflects the generated fluorescence. TheFF410/504/582/669-Di01is ideal for reflecting four bands of excitation light andtransmitting fluorescence fromfour different fluorophoressimultaneously.

Di01-T488/568/638 FF410/504/582/669-Di01

Quadruple-edge Dichroic Beamsplitters (polarization-insensitive; for use at 45°)

Laser-transmitting Dichroic Beamsplitters [1] (polarization-insensitive; for use at 45°)



FF410/504/582/669- 410 nm 381-392 nm 420-460 nm 25.2 mm x 35.6 mm x 1.1 mm Di01-25x36 504 nm 475-495 nm 510-531 nm

582 nm 547-572 nm 589-623 nm669 nm 643-656 nm 677-722 nm



[1] These beamsplitters have high and narrow transmission bands centered on popular laser lines while reflecting fluorescence over the broad reflection bands.They are ideal for high-quality imaging with better than 0.75λ flatness over the full aperture.

[2] Wavelength ranges over which the transmission and average reflection are guaranteed to be above 90%.

Emission Reflection TransmissionColor Filter Band [2] Band [2] Size

Di01-T488/532/638- 499-521 nm 488 nm 25.2 mm x 35.6 mm x 5.0 mm25x36x5.0 543-623 nm 532 nm

653-755 nm 636-640 nm

Di01-T488/532/568/638- 499-521 nm 488 nm 25.2 mm x 35.6 mm x 5.0 mm25x36x5.0 543-556 nm 532 nm

580-622 nm 568 nm652-755 nm 636-640 nm


608-623 nm 594 nm653-755 nm 636-640 nm


652-755 nm 636-640 nm





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The examples at the rightshow two high performancemultiband beamsplitters.The Di01-T488/568/638transmits three differentlaser lines and reflects the generated fluorescence. TheFF410/504/582/669-Di01is ideal for reflecting four bands of excitation light andtransmitting fluorescence fromfour different fluorophoressimultaneously.

Di01-T488/568/638 FF410/504/582/669-Di01

Quadruple-edge Dichroic Beamsplitters (polarization-insensitive; for use at 45°)

Laser-transmitting Dichroic Beamsplitters [1] (polarization-insensitive; for use at 45°)



FF410/504/582/669- 410 nm 381-392 nm 420-460 nm 25.2 mm x 35.6 mm x 1.1 mm Di01-25x36 504 nm 475-495 nm 510-531 nm

582 nm 547-572 nm 589-623 nm669 nm 643-656 nm 677-722 nm



[1] These beamsplitters have high and narrow transmission bands centered on popular laser lines while reflecting fluorescence over the broad reflection bands.They are ideal for high-quality imaging with better than 0.75λ flatness over the full aperture.

[2] Wavelength ranges over which the transmission and average reflection are guaranteed to be above 90%.

Emission Reflection TransmissionColor Filter Band [2] Band [2] Size


653-755 nm 636-640 nm


580-622 nm 568 nm652-755 nm 636-640 nm


608-623 nm 594 nm653-755 nm 636-640 nm


652-755 nm 636-640 nm

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Semrock offers fluorescence filters that enable you to achieve superior performance from your real-time confocal microscope system based on the Yokogawa CSU10 or CSU22 scanner. Like allBrightLine filters, they are made exclusively with hard, ion-beam-sputtered coatings to provideunsurpassed brightness and durability.These filters are compatible with all scan head system configurations, regardless of the microscope, camera, and software platforms you have chosen.

Dichroic Beamsplitters for the Yokogawa CSU10 and CSU22 confocal scannersThese beamsplitters transmit the excitation laser light and reflect the fluorescence from the sam-ple. Because the filters are precisely positioned between the spinning microlens array and pinholearray discs, they have been manufactured to exacting physical and spectral tolerances.The filter dimensionsare 13.0 mm x 15.0 mm x 0.5 mm. (Installation in the CSU22 may only be performed by certain Yokogawa-authorized personnel.)

Emission Filters for the Yokogawa CSU10 and CSU22 confocal scannersThese filters mount outside the CSU head, typically in a filter wheel, and provide the utmost in transmission of the desired fluorescence while blocking the undesired scattered laser light and autofluorescence.The filters are 25.0 mm in diameter and are housed in black-anodized aluminum rings.

Laser-blocking Emission Filters for the Yokogawa CSU22 confocal scanner (inside the scan head)These filters go inside the CSU22 head in the motorized emission-filter slider.The purpose is primarily to block undesiredlaser light, preventing it from exiting the scan head to the camera.The filters are 15.0 mm in diameter and are housed in blackanodized aluminum rings. (Installation in the CSU22 may be performed only by certain Yokogawa-authorized personnel.)

Examples of Popular Fluorophores Used with Common Laser Wavelengths

Blocked Laser Wavelengths Transmission Bands Semrock Part Number

405 nm, 488 nm, 561-568 nm 418-472 nm, 583-650 nm Em01-R405/568-25

405 nm, 442 nm, 561-568 nm, 638-647 nm 458-512 nm, 663-750 nm Em01-R442/647-25

405 nm, 488 nm 503-552 nm Em01-R488-25

514 nm 528-650 nm Em01-R514-25

Blocked Laser Wavelengths Transmission Bands Semrock Part Number

405 nm, 442 nm, 514 nm, 638-647 nm 458-497 nm, 529-620 nm, 667-750 nm Em01-R442/514/647-15

405 nm, 442 nm, 488 nm, 561-568 nm 503-546 nm, 583-700 nm Em01-R488/568-15

Laser Wavelength(s) Popular Fluorophores

405 nm DAPI, Hoechst, Alexa Fluor 405

440, 442 nm CFP, Alexa Fluor 430

488 nm GFP, FITC, Alexa Fluor 488

505, 514 nm YFP

561, 568 nm DsRed, Rhodamine, Cy3, Texas Red, Alexa Fluor 568

635, 638, 647 nm Cy5, Alexa Fluor 633 & 647

For the Yokogawa CSU10 & CSU22 Confocal Scanners

Transmitted Laser Wavelengths Reflection Bands Semrock Part Number

405 nm, 488 nm, 561-568 nm, 638-647 nm 422-473 nm, 503-545 nm, 586-620 nm, 665-750 nm Di01-T405/488/568/647-13x15x0.5

405-442 nm, 514 nm, 638-647 nm 458-497 nm, 533-620 nm, 665-750 nm Di01-T442/514/647-13x15x0.5

405-442 nm, 502-508 nm, 630-641 nm 458-484 nm, 527-607 nm, 664-750 nm Di01-T442/505/635-13x15x0.5

488 nm, 532 nm 442-473 nm, 503-510 nm, 534-750 nm Di01-T488/532-13x15x0.5

488 nm, 568 nm 422-473 nm, 503-545 nm, 586-750 nm Di01-T488/568-13x15x0.5

405-488 nm 508-700 nm Di01-T488-13x15x0.5

For graphs of the filter spectra and ASCII data, go to www.semrock.com.


Notch Filters

32www.semrock.com

The “-25” in the part numbers above indicates these filters are 25 mm in diameter. See table on left for 50 mm diameter filters.

Laser TransitionLine Width [1] Passband Part Number

568.2 nm < 174 cm–1 575.6-1281.7 nm LP02-568RU-25< 345 cm–1 581.3-1281.7 nm LP02-568RS-25










RazorEdge® Raman Filters

Ultra-steep edges to measure even the smallest Raman shifts

Deep laser-line blocking for maximum laser rejection

High signal transmission to detect the weakest signals

High laser damage threshold and proven reliability

Rejected light is reflected, for convenient alignment and best stray-light control

Ultra-wide, low-ripple passband

Semrock RazorEdge thin-film filter technology offers dramatically improved long-wave-pass filters that provide better Stokes-edge steepness and higher transmission than even the leading holographic notch filters, yet are less than half the price!RazorEdge filter technology is so unique that it is patented (U.S. patent No. 7,068,430).

For a perfectly-matchedMaxLine® filter see page 36.

All standard RazorEdgewavelengths available[1]

RazorEdge Long Wave Pass Edge Filters (25 mm Diameter)

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[1] See pages 34 and 38 for more on Transition Width and wavenumbers[2] See additional information on UV RazorEdge filters on page 35

Laser Line Part Number

Long Wave Pass Edge FiltersLP0_-___RU-50LP0_-___RS-50

RazorEdge Long Wave Pass Edge Filters (50 mm Diameter)

Laser TransitionLine Width [1] Passband Part Number

248.6 nm[2] < 805 cm–1 261.0-560.8 nm LP02-248RS-25266.0 nm[2] < 372 cm–1 272.4-600.0 nm LP02-266RU-25

< 737 cm–1 275.0-600.0 nm LP02-266RS-25325.0 nm < 305 cm–1 329.2-432.5 nm LP02-325RU-25








< 381cm–1 526.3-726.3 nm LP01-514RS-25532.0 nm < 186 cm–1 538.9-1200.0 nm LP03-532RU-25

< 369 cm–1 544.2-1200.0 nm LP03-532RS-25


NEW!

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UV Long Wave PassRazorEdge filters – see page 35

NEW!

[1] Except 248.6 and 266 nm filters – call for availability.

Call us: +49 (0)8153 405-032

Edge Filters Edge Filters

Notch Filters

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RazorEdge Short Wave Pass Edge Filters(50 mm Diameter)


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Spectra Examples of RazorEdge Raman FiltersActual measured data from a typical filter is shown!

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The graph to the left shows a typical measured spectrum of a 532 nm RazorEdgefilter plotted on a logarithmic (OD) scale. Note that because RazorEdge filtersare so steep (the steepness measured between the OD 6 and 50% transmissionpoints is typically below 0.5% of the edge wavelength!), standard laboratory spectrophotometers are insufficient for measuring the data accurately. Variousinstrument artifacts appear, including: noise above a certain OD level due to theinstrument noise floor; rounded “corners” of the spectrum due to the non-zerobandwidth of monochromators; and edges that appear less steep than they actually are, especially above OD levels of about 3, due to imperfections in thediffraction gratings and other optics that lead to side bands in the monochroma-tor spectrum. Fortunately precise measurements of specific features of the spectrum near the edge (such as OD at the laser wavelength) can be made morepainstakingly with special equipment.

Transmission spectra of 532 nm RazorEdge filters

These unique filters (U.S. patent No. 7,068,430) are ideal for Anti-Stokes Raman applications. The latest addition to the popularhigh-performance RazorEdge family of steep edge filters, these new short-wave-pass filters are designed to attenuate a designatedlaser-line by six orders of magnitude, and yet maintain a typical edge steepness of only 0.5% of the laser wavelength. Both short-and long-wave-pass RazorEdge filters are perfectly matched to Semrock’s popular MaxLine® laser-line cleanup filters.

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diameter. All wavelengths are also available in 50 mm diameter. See the table below for changes to the part numbers.

All standard RazorEdgewavelengths available

Laser Line Part Number

Short Wave Pass Edge FiltersSP01-___RU-50

632.8 nm RazorEdge Short Wave Pass Filter

RazorEdge Short Wave Pass Edge Filters (25 mm Diameter)

Laser TransitionLine Width Passband Part Number

532.0 nm < 186 cm–1 350.0-525.2 nm SP01-532RU-25632.8 nm < 160 cm–1 350.0-624.6 nm SP01-633RU-25785.0 nm < 129 cm–1 400.0-774.8 nm SP01-785RU-25

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Measured from laser wavelengthto 50% transmission wavelength

[1] For small angles (in degrees), the wavelength shift near the laser wavelength is Δλ (nm) = – 5.0 × 10–5 × λL × θ2 and the wavenumber shift is Δ(wavenumbers)(cm–1) = 500 × θ2 / λL, where λL (in nm) is the laser wavelength. See technical note on page 40.

Common Specifications

RazorEdge® and MaxLine® are a Perfect Match

The MaxLine (see page 36) and RazorEdge filters make an ideal filter pairfor applications like Raman spectroscopy – they fit together like hand-in-glove.The MaxLine filter spectrally “cleans up” the excitation laser lightbefore it reaches the sample under test – allowing only the desired laserline to reach the sample – and then the RazorEdge filter removes the laserline from the light scattered off of the sample, while efficiently transmittingdesired light at wavelengths very close to the laser line.

Typical measured spectral curves of 785 nm filters on a linear transmissionplot demonstrate how the incredibly steep edges and high transmissionexhibited by both of these filters allow them to be spectrally positionedvery close together, while still maintaining complementary transmission andblocking characteristics.

The optical density plot (see page 42) illustrates the complementary natureof these filters on a logarithmic scale using the theoretical design spectralcurves.The MaxLine filter provides very high transmission (> 90%) of lightimmediately in the vicinity of the laser line, and then rapidly rolls off toachieve very high blocking (> OD 5) at wavelengths within 1% of the laser line.The RazorEdge filter provides extremely high blocking (> OD 6)of the laser line itself, and then rapidly climbs to achieve very high transmission (> 90%) of the desired signal light at wavelengths only 1% away from the laser line.


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Edge Steepness (typical) 0.5% of laser wavelength Measured from OD 6 to 50%;(2.5 nm or 90 cm–1 for 532 nm filter) Up to 0.8% for filters below 300 nm

Blocking at Laser Wavelength > 6 OD OD = – log10 (transmission)Transition Width “U” grade < 1% of laser wavelength

(< 5 nm or 180 cm–1 for 532 nm filter)

“S” grade < 2% of laser wavelength(< 10 nm or 360 cm–1 for 532 nm filter)

Average Passband Transmission > 93% > 90% for 248, 266, and 325 nm filterMinimum Passband Transmission > 90% > 80% for 248, 266, and 325 nm filterPassband Wavelength Range See tables on page 32Angle of Incidence 0.0 ± 2.0° See technical note on page 40Cone Half Angle < 5° Rays uniformly distributed about 0° Angle Tuning Range [1] – 0.3% of laser wavelength Wavelength “blue-shift” attained

(– 1.6 nm or + 60 cm–1 for 532 nm filter) by increasing angle from 0° to 8°Temperature Dependence < 5 ppm / °C < 0.003 nm / °C for 532 nm filterLaser Damage Threshold 1 J/cm2 @ 532 nm 10 ns pulse width

0.5 J/cm2 @ 266 nm Tested for 266 and 532 nm filters onlySubstrate Material Fused silica NBK7 or equivalent for LP01 filtersCoating Type “Hard” ion-beam-sputteredClear Aperture ≥ 22 mm (or ≥ 45 mm) ≥ 21 mm for LP01 filtersOuter Diameter 25.0 + 0.0 / – 0.1 mm (or 50.0 + 0.0 / – 0.1 mm) Black-anodized aluminum ringOverall Thickness 3.5 ± 0.1 mmTransmitted Wavefront Error < λ / 4 RMS at λ = 633 nm (per inch) Peak-to-valley error < 5 x RMSBeam Deviation ≤ 30 arc secondsSurface Quality 60-40 scratch-dig 80-50 scratch-dig for LP01 filtersReliability and Durability Ion-beam-sputtered, hard-coating technology with epoxy-free, single-substrate

construction for unrivaled filter life. RazorEdge filters are rigorously tested and provento MIL-STD-810F and MIL-C-48497A environmental standards. See page 3 for details.

Edge Filters Edge Filters

www.laser2000.de 35

Semrock’s renowned and patented (U.S. Patent No. 7,068,430) RazorEdge thin-film filters are now available matched to popularultraviolet (UV) lasers. Due to near-band-edge resonance enhancement effects, and the absence of autofluorescence in the UV,Raman spectroscopy at UV wavelengths can be substantially more sensitive than at visible and near-infrared wavelengths. With theemergence of commercially available UV lasers of sufficient performance, convenience, and price for widespread use, UV Ramanspectroscopy is growing rapidly.

248.6 nm Long Wave Pass Edge FilterIdeal for new, compact NeCu lasers

266.0 nm Long Wave Pass Edge FilterIdeal for 4th Harmonic of Nd:YAG lasers

These unique new UV filters have:

The same ultrasteep edges and deep laser-line blocking of our visible and near-IRRazorEdge filters

Superb UV transmission for detecting the weakest Raman signals


35www.semrock.com


UV Long Wave Pass Edge Filters

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MeasuredLaser Line

Ultraviolet (UV) Raman Spectroscopy

Raman spectroscopy measurements generally face two limitations:(1) Raman scattering cross sections are tiny, requiring intense lasersand sensitive detection systems just to achieve enough signal; and (2) the signal-to-noise ratio is further limited by fundamental, intrinsicnoise sources like sample autofluorescence. Raman measurementsare most commonly performed with green, red, or near-infrared (IR)lasers, largely because of the availability of established lasers anddetectors at these wavelengths. However, by measuring Raman spectra in the ultraviolet (UV) wavelength range, both of the abovelimitations can be substantially alleviated.

Visible and near-IR lasers have photon energies below the first electronic transitions of most molecules. However, when the photonenergy of the laser lies within the electronic spectrum of a molecule,as is the case for UV lasers and most molecules, the intensity of


Raman-active vibrations can increase by many orders of magnitude –this effect is called “resonance-enhanced Raman scattering.”

Further, although UV lasers tend to excite strong autofluorescence,it typically occurs only at wavelengths above about 300 nm,independent of the UV laser wavelength. Since even a 4000 cm –1

(very large) Stokes shift leads to Raman emission below 300 nmwhen excited by a common 266 nm laser, autofluorescence simplydoes not interfere with the Raman signal making high signal-to-noiseratio measurements possible.

Recently an increasing number of compact, affordable, and high-power UV lasers have become widely available, such as quadrupled,diode-pumped Nd:YAG lasers at 266 nm and NeCu hollow-cathodemetal-ion lasers at 248.6 nm, making ultra-sensitive UV Raman spectroscopy a now widely accessible technique.

See page 32 for part numbers and page 34 for specifications.

NEW!

Razo

rEdge

®

Ram

an filters

Edge Filters

3� Call us: +49 (0)8153 405-0

Max

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Las

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Spectra of MaxLine Filters Actual measured data from a typical filter is shown!

Highest laser-line transmission – don’t waste expensive laser light!

Steepest edges – the perfect match to Semrock’s ultra-sharp RazorEdge® filters (see page 32)

Ideal complement to Semrock’s StopLine® deep notch filters (see page 42) for fluorescence and other applications

Hard dielectric coatings for proven reliability and durability

Semrock MaxLine Laser-line Filters have an unprecedented high transmission exceeding 90% at the laser line, while rapidly rollingoff to an optical density (OD) > 5 at wavelengths differing by only 1% from the laser wavelength, and OD > 6 at wavelengths differing by only 1.5% from the laser wavelength. This performance is so exclusive that MaxLine filter technology is patented (U.S.patent No. 7,119,960).

MaxLine® Laser-line Filters

735 745 755 765 775 785 795 805 815 825 8350

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These graphs demonstratethe outstanding performanceof the 785 nm MaxLinelaser-line filter, which hastransmission guaranteed to exceed 90% at the laserline and OD > 5 blockingless than 1% away from the laser line. Note the excellent agreement with the design curves.

Guaranteed Typical OD 5 OD 5 12.5 mm Diameter 25 mm DiameterWavelength Transmission Bandwidth Blue Range Red Range Part Number Part Number

266.0 nm > 55% 1.9 nm 242.8-263.3 nm 268.7-302.2 nm LL01-266-12.5 LL01-266-25325.0 nm > 80% 1.2 nm 291.0-321.8 nm 328.3-380.7 nm LL01-325-12.5 LL01-325-25355.0 nm > 80% 1.3 nm 314.8-351.5 nm 358.6-422.5 nm LL01-355-12.5 LL01-355-25363.8 nm > 85% 1.4 nm 321.7-360.2 nm 367.4-435.0 nm LL01-364-12.5 LL01-364-25441.6 nm > 90% 1.7 nm 381.0-437.2 nm 446.0-551.1 nm LL01-442-12.5 LL01-442-25457.9 nm > 90% 1.7 nm 393.1-453.3 nm 462.5-576.7 nm LL01-458-12.5 LL01-458-25488.0 nm > 90% 1.9 nm 415.1-483.1 nm 492.9-625.3 nm LL01-488-12.5 LL01-488-25514.5 nm > 90% 2.0 nm 434.1-509.4 nm 519.6-669.5 nm LL01-514-12.5 LL01-514-25532.0 nm > 90% 2.0 nm 446.5-526.7 nm 537.3-699.4 nm LL01-532-12.5 LL01-532-25543.5 nm > 90% 2.1 nm 454.6-538.1 nm 548.9-719.5 nm LL01-543-12.5 LL01-543-25561.0 nm > 90% 2.1 nm 466.7-555.4 nm 566.6-750.5 nm LL01-561-12.5 LL01-561-25568.2 nm > 90% 2.2 nm 471.7-562.5 nm 573.9-763.4 nm LL01-568-12.5 LL01-568-25632.8 nm > 90% 2.4 nm 515.4-626.5 nm 639.1-884.7 nm LL01-633-12.5 LL01-633-25647.1 nm > 90% 2.5 nm 524.8-640.6 nm 653.6-912.9 nm LL01-647-12.5 LL01-647-25780.0 nm > 90% 3.0 nm 609.0-772.2 nm 787.8-1201.8 nm LL01-780-12.5 LL01-780-25785.0 nm > 90% 3.0 nm 612.0-777.2 nm 792.9-1213.8 nm LL01-785-12.5 LL01-785-25808.0 nm > 90% 3.1 nm 625.9-799.9 nm 816.1-1139.4 nm LL01-808-12.5 LL01-808-25830.0 nm > 90% 3.2 nm 639.1-821.7 nm 838.3-1183.7 nm LL01-830-12.5 LL01-830-25976.0 nm > 90% 3.7 nm 722.2-966.2 nm 985.8-1325.2 nm LL01-976-12.5 LL01-976-25980.0 nm > 90% 3.7 nm 724.4-970.2 nm 989.8-1332.6 nm LL01-980-12.5 LL01-980-251047.1 nm > 90% 4.0 nm 963.3-1036.6 nm 1057.6-1398.6 nm LL01-1047-12.5 LL01-1047-251064.0 nm > 90% 4.0 nm 978.9-1053.4 nm 1074.6-1428.9 nm LL01-1064-12.5 LL01-1064-25


NEW!

Laser-line Filters

Laser-line Filters


37www.semrock.com


Full Width at Half Maximum (FWHM)Typical 0.7% and Maximum 0.9% for 266 nm filter

[1] The wavelengths associated with these red and blue shifts are given by λ = 1/(1/λL – red shift × 10 –7) and λ = 1/(1/λL + blue shift × 10 –7), respectively, where λ and λL are in nm, and the shifts are in cm –1. Note that the red shifts are 3600 cm–1 for the 808 and 830 nm filters and 2700 cm–1 for the 980 nm filter, and the red and blue shifts are 2400 and 800 cm–1, respectively, for the 1047 and 1064 nm filters. See Technical Note on wavenumbers on page 38.


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Spectra of MaxLine FiltersActual measured data from typical filters is shown!

Illustration of MaxLine filter blocking performance 532 nm filter shown as an example

475425 525 575 625 675500450 550 600 650 700

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OD 5 RedOD 5 RedRRangee

OD 5 BOD 5 BBlueBlueRangee

OD 6 BlueOD 6 BlueORange

OD 6 RedOD 6 RedRange

Limited wavelengthrange shown foreach filter.

MaxL

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Laser-line filters


Laser Wavelength λL Standard laser wavelengths available See page 36 and www.semrock.comTransmission at Laser Line > 90% Except λL < 400 nm; see page 36

Will typically be even higherBandwidth Typical 0.38% of λL

Maximum 0.7% of λL

Blocking[1] OD > 5 from λL ± 1% to 4500 cm–1 OD = – log10 (Transmission)(red shift) and 3600 cm–1 (blue shift);OD > 6 from λL ± 1.5% to 0.92 and 1.10 × λL

Angle of Incidence 0.0 ± 2.0° See technical note on page 40Temperature Dependence < 5 ppm / °C < 0.003 nm / °C for 532 nm filterLaser Damage Threshold 0.1 J/cm2 @ 532 nm (10 ns pulse width) Tested for 532 nm filter onlySubstrate Material Low autofluorescence NBK7 or better Fused silica for 266 nm filterCoating Type “Hard” ion-beam-sputteredClear Aperture ≥ 10 mm (or ≥ 22 mm) For all optical specificationsOuter Diameter 12.5 + 0.0 / – 0.1 mm Black-anodized aluminum ring

(or 25.0 + 0.0 / – 0.1 mm)Overall Thickness 3.5 ± 0.1 mmTransmitted Wavefront Error < λ / 4 RMS at = λ 633 nm Peak-to-valley error < 5 × RMSBeam Deviation ≤ 11 arc secondsSurface Quality 60-40 scratch-dig Measured within clear apertureReliability and Durability Ion-beam-sputtered, hard-coating technology with epoxy-free, single-substrate

construction for unrivaled filter life. MaxLine filters are rigorously tested and provento MIL-STD-810F and MIL-C-48497A environmental standards. See page 3 for details.

Laser-line Filters

38 Call us: +49 (0)8153 405-0


The “color” of light is generally identified by the distribution ofpower or intensity as a function of wavelength λ. For example,visible light has a wavelength that ranges from about 400 nm tojust over 700 nm. However, sometimes it is convenient todescribe light in terms of units called “wavenumbers,” where the wavenumber w is typically measured in units of cm−1

(“inverse centimeters”) and is simply equal to the inverse ofthe wavelength:

In applications like Raman spectroscopy, often both wavelengthand wavenumber units are used together, leading to potential con-fusion. For example, laser lines are generally identified by wave-length, but the separation of a particular Raman line from thelaser line is generally given by a “wavenumber shift” Δw, since thisquantity is fixed by the molecular properties of the material andindependent of which laser wavelength is used to excite the line.


When speaking of a “shift” from a first known wavelength λ1 to asecond known wavelength λ2 , the resulting wavelength shift Δλ isgiven by

whereas the resulting wavenumber shift Δw is given by

When speaking of a known wavenumber shift Δw from a firstknown wavelength λ1, the resulting second wavelength λ2 is given by

Note that when the final wavelength λ2 is longer than the initialwavelength λ1, which corresponds to a “red shift,” in the aboveequations Δw < 0, consistent with a shift toward smaller values of w. However, when the final wavelength λ2 is shorter than theinitial wavelength λ1, which corresponds to a “blue shift,” Δw > 0,consistent with a shift toward larger values of w.

Measuring Light with Wavelengths and Wavenumbers

107

λ(nm)w(cm−1) =

Δλ = λ2 − λ1

1λ2

Δw = 1λ1

× 10 7 Δλλ1λ2

− × 107−( )

11/λ1 + Δw × 10−7

λ2 =

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33,33350,000 20,00025,000 16,667 14,286 12,500 10,00011,111 9,091 8,333

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=

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See page 36 for part numbers and page 37 for specifications.

High Efficiency Narrowband Laser Clean-up Filters for 266 nmNEW!

Semrock’s highly acclaimed MaxLine thin-film filters (U.S. Patent No. 7,119,960) are now available for the popular 266 nm quadrupled Nd:YAG laser. These unique new UV filters have:

Unprecedented UV transmission – stop wasting expensive UV laser light!

The same rapid roll-off and deep blocking of our visible and near-IR MaxLine filters

A perfect match to our new 266 nm RazorEdge filters (page 35)

Proven reliability

266.0 nm Narrowband Laser-line FilterEfficiently clean up your quadrupled Nd:YAG Laser

Max

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Laser-line Filters

Laser-line Filters

www.laser2000.de 39

Filter Types for Raman Spectroscopy Applications

Raman spectroscopy is widely used today for applications ranging from industrial process control to laboratory research to bio/chemicaldefense measures. Industries that benefit from this highly specific analysis technique include the chemical, polymer, pharmaceutical, semi-conductor, gemology, computer hard disk, and medicalfields. In Raman spectroscopy, an intense laser beam isused to create Raman (inelastic) scattered light from asample under test. The Raman “finger print” is measuredby a dispersive or Fourier Transform spectrometer.

There are three basic types of Raman instrumentation. Raman microscopes, also called micro-Raman spectrophotometers, are larger-scale laboratoryanalytical instruments for making fast, high-accuracyRaman measurements on very small, specific sampleareas. Traditional laboratory Raman spectrometers areprimarily used for R&D applications, and range from“home-built” to flexible commercial systems that offer a variety of laser sources, means for holding solid and liquid samples, and different filter and spectrometer types. Finally, a rapidly emerging class of Raman instrumentation is the Raman micro-probe analyzer.These complete, compact and often portable systems are ideal for use in the field or in tight manufacturing and process environments.They utilize a remote probe tip that contains optical filters and lenses, connected to the main unit via optical fiber.

Optical filters are critical components in Raman spectroscopy systems to prevent all undesired light from reaching the spectrometerand swamping the relatively weak Raman signal. Laser Transmitting Filters inserted between the laser and the sample block all undesiredlight from the laser (such as broadband spontaneous emission or plasma lines) as well as any Raman scattering or fluorescence generatedbetween the laser and the sample (as in a fiber micro-probe system). Laser Blocking Filters inserted between the sample and the spec-trometer block the Rayleigh (elastic) scattered light at the laser wavelength.

There are three basic types of filters to choose from: Laser-line Filters, Edge Filters, and Notch Filters. The examples below show howthe various filters are used. In these graphs the blue lines represent the filter transmission spectra, the green lines represent the laserspectrum, and the red lines represent the Raman signal.

Laser-Line Filters are ideal for use as Laser Transmitting Filters, and Notch Filters are an obvious choice for Laser Blocking Filters. In systems using these two filter types, both Stokes and Anti-Stokes Raman scattering can be measured simultaneously. However, in manycases Edge Filters provide a superior alternative. For example, a long-wave-pass (LWP) Edge Filter used as a Laser Blocking Filter formeasuring Stokes scattering offers better transmission, higher laser-line blocking, and the steepest edge performance to see Ramansignals extremely close to the laser line. For more details on choosing between edge filters and notch filters, see the Technical Note “Edge Filters vs. Notch Filters for Raman Instrumentation” on page 44.

Semrock stocks high-performance MaxLine® Laser-line Filters, RazorEdge® long-wave-pass and short-wave-pass Edge Filters, and StopLine® Notch Filters as standard catalog products. Non-standard wavelengths and specifications for these filters are routinely manufactured for volume OEM applications.


Sample

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Laser-line Filter LWP Edge Filter

Laser-transmitting filter for both Stokes andAnti-Stokes measurements

Laser-blocking steep edge filter for superiorStokes measurements

Tra

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Wavelength

Notch Filter

Versatile laser-blocking notch filter for bothStokes and Anti-Stokes measurements

Laser-line Filters

40 Call us: +49 (0)8153 405-0

Filter Spectra at Non-normal Angles of Incidence

Many of the filters in this catalog (with the exception of dichroic beamsplitters and the MaxMirror®) are optimized for use with lightat or near normal incidence. However, for some applications it is desirable to understand how the spectral properties change for anon-zero angle of incidence (AOI).

There are two main effects exhibited by the filter spectrum as the angle is increased from normal:

1. the features of the spectrum shift to shorter wavelengths;2. two distinct spectra emerge – one for s-polarized light and one for p-polarized light.

As an example, the graph at the right shows a series of spectra derived from a typical RazorEdge long-wave-pass (LWP) filter design. Because the designs areso similar for all of the RazorEdge filters in the series, the set of curves in thegraph can be applied approximately to any of the filters. Here the wavelength λis compared to the wavelength λ0 of a particular spectral feature (in this casethe edge location) at normal incidence. As can be seen from the spectralcurves, as the angle is increased from normal incidence the filter edge shiftstoward shorter wavelengths and the edges associated with s- and p-polarizedlight shift by different amounts. For LWP filters, the edge associated with p-polarized light shifts more than the edge associated with s-polarized light,whereas for short-wave-pass (SWP) filters the opposite is true. Because of this polarization splitting, the spectrum for unpolarized light demonstrates a “shelf” near the 50% transmission point when the splitting significantly exceeds the edge steepness. However, the edge steepness for polarized light remains very high.

The shift of almost any spectral feature can be approximately quantified by a simple model of the wavelength λ of the feature vs. angle of incidence θ,given by the equation:

where neff is called the effective index of refraction, and λ0 is the wavelength of the spectral feature of interest at normal incidence. Different shifts thatoccur for different spectral features and different filters are described by a different effective index. For the RazorEdge example above, the shift of the 90% transmission point on the edge is described by this equation with neff = 2.08 and 1.62 for s- and p-polarized light, respectively.

Other types of filters don’t necessarily exhibit such a marked difference in the shift of features for s- and p-polarized light. For example, the middle graphshows a series of spectra derived from a typical MaxLine laser-line filter design curve. As the angle is increased from normal incidence, the center wavelength shifts toward shorter wavelengths and the bandwidth broadens slightly for p-polarized light while narrowing for s-polarized light.The centerwavelength shifts are described by the above equation with neff = 2.19 and 2.13 for s- and p-polarized light, respectively.The most striking feature is the decrease in transmission for s-polarized light, whereas the transmissionremains quite high for p-polarized light.

As another example, the graph at the right shows a series of spectra derived from a typical StopLine notch filter design curve. As the angle isincreased from normal incidence, the notch center wavelength shifts to shorter wavelengths, the notch depth decreases, and the notch bandwidthdecreases (with a greater decrease for p-polarized light than for s-polarizedlight).The shift of the notch center wavelength is described by the above equation with neff = 1.76 for both s- and p-polarized light. Note that it is possible to optimize the design of a notch filter to have a very deep notch even at a 45° angle of incidence.


0.88 0.90 0.92 0.94 0.96 0.98 1.00 1.02 1.04 1.06 1.080

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λ 1 – (sinθ/neff)2

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Clean-up Filters

www.laser2000.de 41

Square low-ripple passband for total consistency as the laser ages,over temperature, or when replacing a laser

Highest transmission exceeding 90%, over a carefully tailored range of each diode’s possible laser wavelengths

Extremely steep edges transitioning to very high blocking to filter out the undesired out-of-band noise

MaxDiode filters have an exceptionally high-transmission, low-ripple passband for consistent high throughput.This remarkablepassband is further defined by steep edges for high optical noise discrimination. Keep the desirable laser light while eliminatingthe noise! The MaxDiode filters are ideal for both volume OEM manufacturers of laser-based fluorescence instrumentation andlaboratory researchers who use diode lasers for fluorescence excitation and other types of spectroscopic applications.

MaxDiode™ Laser Diode Clean-up Filters

Spectra of MaxDiode Laser Diode Clean-up Filters Actual measured data from typical filters is shown!


500300 700600400

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Transmission over Full Bandwidth > 90% Will typically be even higher Transmission Ripple < ± 1.5% Measured peak-to-peak across bandwidthBlocking Wavelength Ranges Optimized to eliminate spontaneous emission See table aboveAngle of Incidence 0.0 ± 5.0° Range for above optical specificationsPerformance for Non-collimated Light The high-transmission portion of the long-wavelength edge and the low-transmission portion of the

short-wavelength edge exhibit a small “blue shift” (shift toward shorter wavelengths). Even for Cone Half Angles as large as 15° at normal incidence, the blue shift is only several nm.

Filter Orientation Arrow on ring indicates direction of propagation of laser light

Wavelength (nm)

470 nm Laser0Rangen

OD 3 RedOD 3 RedRangeg

OD 3 BlueOD 3 BlueRangeg

OD 5 BlueOD 5 BlueeRangeg

OD 5 RedOD 5 RedRangeg

Design

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Illustration of MaxDiode filter blocking performance 470 nm filter shown as an example

All other mechanical specifications are the same as MaxLine® specifications on page 37.

MaxD

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Laser-dio

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Laser Diode Transmission Center OD 3 OD 5 12.5 mm 25 mmWavelength & Bandwidth Wavelength Color Range Color Range Part Number Part Number

375 nm > 90% over 6 nm 375 nm 212-365 & 385-554 nm 337-359 & 393-415 nm LD01-375/6-12.5 LD01-375/6-25405 nm > 90% over 10 nm 405 nm 358-389 & 420-466 nm 361-384 & 428-457 nm LD01-405/10-12.5 LD01-405/10-25 440 nm > 90% over 8 nm 439 nm 281-425 & 453-609 nm 392-422 & 456-499 nm LD01-439/8-12.5 LD01-439/8-25470 nm > 90% over 10 nm 473 nm 308-458 & 488-638 nm 423-455 & 491-537 nm LD01-473/10-12.5 LD01-473/10-25635 nm > 90% over 8 nm 640 nm 400-625 & 655-720 nm 580-622 & 658-717 nm LD01-640/8-12.5 LD01-640/8-25


NEW!

Clean-up Filters

42 Call us: +49 (0)8153 405-0

Deep laser-line blocking for maximum laser rejection

Narrowest bandwidth thin-film notch filters

High signal transmission to detect the weakest signals

Negligible temperature dependence for maximum temperature stability


Rejected light is reflected, for convenient alignment and best stray-light control

Semrock’s StopLine Deep Notch Filters rival the performance of holographic notch filters but in a less expensive, more convenient, and more reliable thin-film filter format.These filters are ideal for applications including Raman spectroscopy, laser-based fluorescence instruments, and biomedical laser systems. Multi-notch filters are also available (see page 45).These filtersare so advanced they are patented (U.S. patent No. 7,123,416).

StopLine® Notch Filters

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632.8 nm StopLine Filter

Spectra of StopLine Single-notch FiltersActual measured data from a typical filter is shown!

Working with Optical Density

Optical Density – or OD, as it is commonly called – is a convenient tool to describe the transmission of light through a highly blocking opticalfilter (when the transmission is extremely small). OD is simply defined as the negative of the logarithm (base 10) of the transmission, wherethe transmission varies between 0 and 1 (OD = – log10(T)). Therefore, the transmission is simply 10 raised to the power of minus the OD (T = 10 – OD). The graph below left demonstrates the power of OD: a variation in transmission of six orders of magnitude (1,000,000times) is described very simply by OD values ranging between 0 and 6. The table of examples below middle, and the list of “rules” belowright, provide some handy tips for quickly converting between OD and transmission. Multiplying and dividing the transmission by two and ten is equivalent to subtracting and adding 0.3 and 1 in OD, respectively.


Transmission OD

1 00.5 0.30.2 0.70.1 10.05 1.30.02 1.70.01 20.005 2.30.002 2.70.001 3

The “1” RuleT = 1 → OD = 0

The “x 2” RuleT x 2 → OD – 0.3

The “÷ 2” RuleT ÷ 2 → OD + 0.3

The “x 10” RuleT x 10 → OD – 1

The “÷ 10” RuleT ÷ 10 → OD + 1

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Wavelength Bandwidth Blocking Part Number

405.0 nm 9 nm OD > 6 NF02-405U-2512 nm OD > 4 NF02-405S-25

441.6 nm 11 nm OD > 6 NF01-442U-2514 nm OD > 4 NF02-442S-25

488.0 nm 14 nm OD > 6 NF01-488U-25514.5 nm 16 nm OD > 6 NF01-514U-25526.5 nm 17 nm OD > 6 NFO1-526U-25532.0 nm 17 nm OD > 6 NF01-532U-25

20 nm OD > 4 NF02-532S-25568.2 nm 20 nm OD > 6 NF01-568U-25594.1 nm 22 nm OD > 6 NF01-594U-25632.8 nm 25 nm OD > 6 NF01-633U-25

29 nm OD > 4 NF02-633S-25785.0 nm 39 nm OD > 6 NF01-785U-25808.0 nm 41 nm OD > 6 NF01-808U-25830.0 nm 44 nm OD > 6 NF01-830U-25


NEW!

Notch Filters Notch Filters

www.laser2000.de 43

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950

405.0 nm StopLine Filter 488.0 nm StopLine Filter



594.1 nm StopLine Filter 632.8 nm StopLine Filter*568.2 nm StopLine Filter

Design

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Spectra of StopLine Single-notch Filters

* Measurement note: Due to the extreme performance of these StopLine notch filters (narrow and very deep notches), it is difficult and time consuming to obtain accurate logarithmic scale measurements, even with the use of a double monochromator. Semrock has carefully measured a typical 633 nm deep notch filter and compared it with the calculated performance (see graph above and on page 42). You can see that the agreement is exceptional – a signature characteristic of Semrock’s highly controlled ion-beam sputtering manufacturing process. Therefore, while we display only theoretical curves for the remaining deep notch filters, we are confident that these are representative curves.

Design

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For complete ASCII data and the latest offerings, go to www.semrock.com.

NEW!

www.semrock.com

Notch Filters

44 Call us: +49 (0)8153 405-0

Sto

pLin

e®

No

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rs

[1] For NF02-405 filter, 90% bandwidth is < 1.3 × Maximum 50% Bandwidth, and Passband short wavelength is 330 nm.[2] For small angles θ (in degrees), the wavelength shift near the laser wavelength is Δλ (nm) = – 5.0 × 10–5 × λL × θ2 and the wavenumber

shift is Δ(wavenumbers) (cm–1) = 500 × θ2 / λL, where λL (in nm) is the laser wavelength.

Full width at 50% transmission; λL is design laser wavelength(NBW and λL in nm)

At the design laser wavelength;OD = – log10 (transmission)


Laser Line Blocking: “U” grade > 6 OD“S” grade > 4 OD

Typical 50% Notch “U” grade NBW = 55 × 10–6 × λL2 + 14 × 10–3 × λL – 5.9

Bandwidth e.g. 17 nm (600 cm–1) for 532.0 nm filter

“S” grade NBW = 10 × 10–5 × λL2 – 29 × 10–3 × λL + 7.2

e.g. 20 nm (700 cm–1) for 532.0 nm filter

Maximum 50% Notch Bandwidth < 1.1 × NBW90% Notch Bandwidth < 1.3 × NBW [1] Full width at 90% transmissionPassband from 0.75 × λL to λL / 0.75 [1] λL is design laser wavelength (nm)Average Passband Transmission > 90%Passband Transmission Ripple < 2.5% Calculated as standard deviationAngle of Incidence 0.0 ± 5.0° See technical note on page 40Angle Tuning Range [2] – 1% of laser wavelength Wavelength “blue-shift” attained

(– 5.3 nm or + 190 cm–1 for 532 nm filter) by increasing angle from 0° to 14°Temperature Dependence < 5 ppm / °C < 0.003 nm / °C for 532 nm filterLaser Damage Threshold 1 J/cm2 @ 532 nm (10 ns pulse width) Tested for 532 nm filter onlySubstrate Material Ultra-low autofluorescence fused silicaCoating Type “Hard” ion-beam-sputteredClear Aperture ≥ 22 mm For all optical specificationsOuter Diameter 25.0 + 0.0 / – 0.1 mm Black-anodized aluminum ringOverall Thickness 3.5 ± 0.1 mmTransmitted Wavefront Error < λ / 4 RMS at = λ 633 nm Peak-to-valley error < 5 × RMSSurface Quality 60-40 scratch-dig Measured within Clear ApertureReliability and Durability Ion-beam-sputtered, hard-coating technology with epoxy-free, single-substrate

construction for unrivaled filter life. StopLine filters are rigorously tested and provento MIL-STD-810F and MIL-C-48497A environmental standards. See page 3 for details.


Common Specifications for Single-notch Filters

Edge Filters vs. Notch Filters for Raman Instrumentation

RazorEdge Filter Advantages (page 32):• Steepest possible edge for looking at the smallest Stokes shifts • Largest blocking of the laser line for maximum laser rejection

StopLine Notch Filter Advantages (page 42):• Measure Stokes and Anti-Stokes signals simultaneously • Greater angle-tunability and bandwidth for use with variable laser lines

The graph below left illustrates the ability of a long-wave-pass (LWP) filter to get extremely close to the laser line. The graph in the centercompares the steepness of an edge filter to that of a notch filter. A steeper edge means a narrower transition width from the laser line to the high-transmission region of the filter. With transition widths guaranteed to be below 1% of the laser wavelength (on Semrock U-gradeedge filters), these filters don't need to be angle-tuned!

The graph on the right shows the relative tuning ranges that can be achieved for edge filters and notch filters. Semrock edge filters can betuned up to 0.3% of the laser wavelength.The filters shift toward shorter wavelengths as the angle of incidence is increased from 0° to about 8°. Semrock notch filters can be tuned up to 1.0% of the laser wavelength.These filters also shift toward shorter wavelengths as the angle of incidence is increased from 0° up to about 14°.

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Notch Filters Notch Filters

www.laser2000.de 45

Sto

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StopLine multi-notch catalog filters include:

StopLine filters are housed in a 25 mm black-anodized aluminum ring unless indicated otherwise. All filters are on low-autofluorescenceNBK7 or ultra-low-autofluorescence fused silica substrates.

Laser-based fluorescence instruments

Confocal and multi-photon fluorescence microscopes

Analytical and medical laser systems

Semrock’s advanced manufacturing process means that we can make these filters with notch wavelengths that are not integermultiples of each other!

Semrock’s unique multi-notch filters meet or exceed even the most demanding requirements of our OEM customers. These includedual-, triple-, and even quadruple-notch filters for a variety of multi-laser instruments. Applications include:

Examples of StopLine Multi-notch Filters – Actual measured data from typical filters is shown!

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405 & 488 & 568 nm Multi-notch Filter

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400-410, 488, 532, & 631-640 nm Multi-notch Filter

For complete graphs, ASCII data, and the latest offerings, go to www.semrock.com.


StopLine Multi-notch Filters

Laser Colors Laser Wavelengths Laser-line Blocking Part Number Dimensions

Dual-notch Filters

488 & 532 nm OD > 6 NF01-488/532-25x5.0 25 x 5.0 mm

488 & 543 nm OD > 6 NF01-488/543-25x5.0 25 x 5.0 mm

486-490 & 631-640 nm OD > 4 NF01-488/635-25.4x6.0-D 25.4 x 6.0 mm(Note: no housing)

488 & 647 nm OD > 6 NF01-488/647-25x5.0 25 x 5.0 mm

543 & 647 nm OD > 6 NF01-543/647-25x5.0 25 x 5.0 mm

568 & 638 nm OD > 6 NF01-568/638-25x5.0 25 x 5.0 mm

568 & 647 nm OD > 6 NF01-568/647-25x5.0 25 x 5.0 mm

594 & 638 nm OD > 6 NF01-594/638-25x5.0 25 x 5.0 mm

Triple-notch Filters

405, 488, & 568 nm OD > 6 NF01-405/488/568-25x3.5 25 x 3.5 mm

488, 532, & 631-640 nm OD > 4 NF01-488/532/635-25x5.0 25 x 5.0 mm

Quadruple-notch Filters

400-410, 488, 532, & 631-640 nm OD > 4 NF01-405/488/532/635-25x5.0 25 x 5.0 mm

400-410, 488, 561, & 631-640 nm OD > 4 NF01-405/488/561/635-25x5.0 25 x 5.0 mm


Notch Filters

4� Call us: +49 (0)8153 405-0

Laser Damage Threshold

Laser damage to optical filters is strongly dependent on many factors, and thus it is difficult to guarantee the performance of a filter in allpossible circumstances. Nevertheless, it is useful to identify a Laser Damage Threshold (LDT) of pulse fluence or intensity below which nodamage is likely to occur.

Pulsed vs. continuous-wave lasers: Pulsed lasers emit light in a series of pulses of duration τ at a repetition rate R, with peak powerPpeak. Continuous-wave (cw) lasers emit a steady beam of light with a constant power. Pulsed-laser average power Pavg and cw laser constantpower typically range from several milli-Watts (mW) to Watts (W) for most lasers. The diagram and table below illustrate and summarizethe key parameters that are used to characterize the output of pulsed lasers.


Symbol Definition Units Key Relationships

τ Pulse duration sec τ = D / R

R Repetition rate Hz = sec–1 R = D / τD Duty cycle dimensionless D = R x τP Power Watts = Joules / sec Ppeak = E / τ; Pavg = Ppeak x D; Pavg = E x R

E Energy per pulse Joules E = Ppeak x τ; E = Pavg / R

A Area of laser spot cm2 A = (π / 4) x diameter2

I Intensity Watts / cm2 I = P / A; Ipeak = F / τ; Iavg = Ipeak x D; Iavg = F x R

F Fluence per pulse Joules / cm2 F = E / A; F = Ipeak x τ; F = Iavg / R

Note that because fluence and intensity on the surface of the component are the critical parameters, the area of the laser spot is also critical. Even very high-power lasers may be transmitted through,or reflected off of, a durable optical filter if the spot size is sufficientlylarge to minimize the fluence and/or intensity. The diameter of a laserspot with a Gaussian profile is most commonly measured at the 1/e2

intensity points as shown in the diagram below.

Long-pulse lasers: LDT is perhaps most accurately specified in terms of pulse fluence for “long-pulse lasers.” Long-pulse lasers have pulse durations τ in thenanosecond (ns) to microsecond (μs) range, with repetition rates R typicallyranging from about 1 to 100 Hz. Because the time between pulses is so large(milliseconds), the irradiated material is able to thermally relax – as a resultdamage is generally not heat-induced, but rather caused by nearly instantaneous optical field effects. Usually damage results from surface or volume imperfections in the material and the associated irregular optical field properties near these sites, rather than catastrophic destruction of the fundamental material structure. Most Semrock filters have LDT values on the order of 1 J/cm2, and are thus considered “high-power laser quality” components. An important exception is aHigh-Q laser-line filter in which the internal field strength is strongly magnified, resulting in an LDT that may be an order of magnitudesmaller.

As an example, suppose a frequency-doubled Nd:YAG laser at 532 nm emits 10 ns pulses at a 10 Hz repetition rate with 1W of averagepower. This laser has a duty cycle of 1 x 10–7, a pulse energy of 100 mJ, and a peak power of 100 MW. If the beam is focused down to a100 μm diameter spot on the surface of a component, the pulse fluence is 1.3 kJ/cm2, and thus it will almost surely damage a componentwith a 1 J/cm2 LDT. However, if the spot diameter is 5 mm, the pulse fluence is only 0.5 J/cm2, and thus the component should not be damaged.

cw lasers: The LDT for cw lasers is more difficult to measure, and therefore is not specified as often as the long-pulse laser LDT.Damage from cw lasers tends to result from thermal (heating) effects. At this time Semrock does not test nor specify cw LDT for its fil-ters. As a very rough rule of thumb, many all-glass components like dielectric thin-film mirrors and filters have a cw LDT (specified asintensity in kW/cm2) that is 10 – 100 times the long-pulse laser LDT (specified as fluence in J/cm2).

Quasi-cw lasers: Quasi-cw lasers are pulsed lasers with pulse durations τ in the femtosecond (fs) to picosecond (ps) range, and withrepetition rates R typically ranging from about 10 – 100 MHz for high-power lasers. These lasers are typically mode-locked, which meansthat R is determined by the round-trip time for light within the laser cavity. With such high repetition rates, the time between pulses is soshort that thermal relaxation cannot occur. Thus quasi-cw lasers are often treated approximately like cw lasers with respect to LDT, usingthe average intensity in place of the cw intensity.

time

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MaxMirror®


Very highly reflecting over:

Near-UV, all Visible, andNear-IR wavelengths

All states of polarization

All angles from 0 to 50° inclusive

simultaneously!


Low-scattering

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300 400 500 600 700 800 900 1000 1100 1200

“s” polarization“p” polarization

Measured at 45˚Also highly reflecting 0 – 50˚

Typical MaxMirror spectrumActual measured data shown.

The MaxMirror is a unique high-performance laser mirror that covers an ultra-broad range of wavelengths – it can replace three or more conventional laser mirrors. In fact, it is so unique that it is patented(U.S. patent No. 6,894,838). The MaxMirror is a winner of the prestigious Photonics Circle of Excellence award,reserved for the most innovative new products of the year. And there is still nothing else like it on the market!

MaxMirror® Ultra-broadband High-Performance Mirror

Win

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Surface Mirror SideDiameter Flatness Part Number

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Wavelength Range 350-1100 nmStandard Reflectivity > 99.0% For unpolarized light

> 98.5% (> 99% typical) For “s” polarization> 98.5% (> 99% typical) For “p” polarization

Standard Angle of Incidence 45.0 ± 2.5° Range over which Standard 0.0 ± 5.0° Reflectivity specifications are met

Wide Angle Reflectivity R > 98.5% For unpolarized lightR > 98.0% For “s” polarizationR > 98.0% For “p” polarization

Wide Angle of Incidence Range 0.0-50.0° Range over which Wide AngleReflectivity specifications are met

Laser Damage Threshold 1 J/cm2 @ 355 and 532 nm ~ 10 ns pulse width2 J/cm2 @ 1064 nm

Substrate Material NBK7 or equivalentCoating Type “Hard” ion-beam-sputteredClear Aperture > 80% of Outer DiameterOuter Diameter 25.0 or 25.4 or 50.8 mm + 0.0 / – 0.25 mmThickness 9.52 ± 0.25 mmMirror Side Surface Flatness See table below Measured at λ = 633 nmMirror Side Surface Quality 20-10 scratch-dig Measured within Clear ApertureMirror Side Bevel 0.75 mm maximumPulse Dispersion The MaxMirror will not introduce appreciable pulse broadening for most laser pulses

that are > 1 picosecond; however, pulse distortion is likely for significantly shorter laser pulses, including femtosecond pulses.

Reliability and Durability Ion-beam-sputtered, hard-coating technology with unrivaled filter life. MaxMirror ultra-broadband mirrors are rigorously tested and proven to MIL-STD-810F and MIL-C-48497A environmental standards; see page 3 for details.

MaxMirror®

fs-Lasers

48 Call us: +49 (0)8153 405-0

Your Partner in the

European Photonics Market

Laser, Lightsources & Accessories

Whether products for industry or for research, Laser 2000 recognizes and understands the customer’s needs. We supply OEM services and provide all solution related components that can be built around or be integrated into the application of your customer.

Gas lasersSolid state lasers Laser diodes and laser diode modules Measurement and control equipment Optical, opto-mechanical and safety products

Image Processing & Machine Vision

To improve productivity and quality in industrial environments we sup-port the increasing demand for pho-tonics products. Our engineers assist customers in selecting the appropri-ate combination of light source, ca-mera and software.

Light sourcesLine and array camera systems Test charts and calibration patternsSoftware

Fiber Optics & Digital Testing

Customers generate profit from a full range of components and systems. Our sales engineers assist in develo-ping solutions. Our seminars are re-cognized as a source of leading edge information.

Active and passive components for Telecom and Datacom Instrumentation for Telecom and Datacom Field test and measurement equip-ment for Optical Networking

Over more than 20 years Laser 2000 is concentrating on the three core product areas of Photonics market. Our experts and experienced account managers ensure the best possible attention to your requirements today for the solutions of tomorrow.

www.laser2000.de 49

Femtosecond Lasers

fs-Lasers

t-Pulse s-Pulse

Specifications t-Pulse 20 t-Pulse 200 t-Pulse 500 s-Pulse s-Pulse HRs-Pulse

HPWavelength 1030 nm 1030 nm 1030 nm 1030 nm 1030 nm 1030 nm

Pulse Energy 20 nJ 200 nJ 500 nJ 100 µJ 10 µJ 30 µJ

Repetition Rate 50 MHz 10 MHz 10 MHz 1…10 kHz 100 kHz 100 kHz

Average Power 1 W 2 W 5 W 0,1…1 W 1 W 3 W

Pulse Duration < 200 fs < 400 fs < 500 fs < 400 fs < 500 fs < 500 fs

Peak Power > 100 kW > 500 kW > 1 MW > 250 MW > 20 MW > 60 MW

Foot print 60 x 20 cm 60 x 40 cm 75 x 50 cm

Pulse Picker x x x x x x

Second Harmonic Generation (SHG) x x x x x x

Third Harmonic Generation (THG) x x x x x x

Pulse Compression x x

Tunability x

The new generation of ultrafast lasers from Amplitude Sys-temes provides diode-pumped femtosecond lasers setting new standards for performance and easy to use. They take full advantage of new, high quality ytterbium doped materi-als. Ytterbium lasers can be directly diode-pumped and do not require any additional green pump laser. They also offer a number of attractive properties, such as a very high thermal efficiency, as well as the capability to generate femtosecond pulses with a high average power.

t-Pulse series

Femtosecond laser t-Pulse 20

The t-Pulse series provides compact and reliable femtose-cond lasers with high average power, exceptional energy per pulse and excellent pulse-to-pulse stability. With its low electrical consumption and its internal cooling system, the t-Pulse is designed for simple daily operation, a short warm-up time and an excellent day to day repeatability. It is the laser of choice for applications like

Multiphoton spectroscopy and microscopy

Photoacoustics

Terahertz imaging

Photopolymerization

Non linear optics

s-Pulse seriesThe s-Pulse series of amplified femtosecond lasers offers high pulse energies at high repetition rates. The s-Pulse is built on a compact, small footprint, rugged mechanical base for improved stability. It features simple control electronics and no requirement for external water cooling. Low electrical consumption and long lifetime laser diodes ensure operating costs are at a minimum. The short pulse duration and the high output beam quality makes the s-Pulse series ideal for

Laser ablation

Marking and engraving

Laser assisted ICP-MS

Eye surgery

Non linear optics

DsRed GFP & Covalaria

YFP GFP

Micro-structures on metal, ceramics

and wafers

Product SpecialistsMark Drechsler+49 (0) [email protected]

Dr. Stefan Kremser+49 (0) [email protected]


50 Call us: +49 (0)8153 405-0

DPSS Lasers DPSS Lasers

Diode pumped solid state lasers

Blue, green and yellow SLIM DPSS lasers

SLM: Low-noise and single longitudinal mode cw operation

Ruggedness: The laser cavity cannot be misaligned by thermal or mechanical stress.

Reliability: Highly reliable GaAs laser diodes are used to pump the lasing medium.

Highest compactness: No micro-mechanical positioning or aligning element is required; the size of the optical laser head can be less than a cubic inch.

Efficiency: Because high internal intensities are achievab-le, frequency doubling can be efficient with pump powers as low as one Watt.

Scalable power levels: Due to the thermal properties of crystals, Watt-scale operation can be achieved without de-gradation.

High spectral purity: With AMR’s millimeter-scale cavity length, single longitudinal mode (SLM) operation can easily be achieved, with a linewidth smaller than 1 MHz and a high signal-to-noise ratio.

Versatility: With AMR, numerous laser wavelengths can be achieved and will be introduced in the near future.

The SLIM platform is a new groundbreaking generation of continuous-wave laser modules featuring outstanding opti-cal performances in a miniaturized package. Based on Oxxi-us‘ proprietary Alignment-free Monolithic Resonator (AMR) technology, it delivers light beams with characteristics par-ticularly well suited to a wide range of instrumentation and scientific applications: single-frequency spectrum, low noise and long-term power stability.

Because of its unparalleled compactness, it is a unique yet affordable light source for a large variety of applications inclu-ding laser induced fluorescence microscopy, flow cytometry, Raman spectroscopy, metrology, holography, display and projection.

All members of the SLIM family have a market-leading size which makes possible the development of reduced-footprint instruments. In addition, because of their low power consump-tion, they can be embedded in mobile or portable devices.

Lasers based on the SLIM platform are available today at 473 nm, 532 nm and 561 nm. They can be provided in beam-shaping and packaging configurations optimized to their target environment.

SLIM-473 SLIM-532 SLIM-561

Parameters Uncollimated Collimated Uncollimated Collimated Uncollimated Collimated

Wavelength 473 nm 532 nm 561 nm

CW Power 20 / 40 mW up to 150 mW up to 25 mW

Power Stability +/- 1% over 24 hours +/- 1% over 24 hours +/- 1% over 24 hours

Optical Noise (RMS, 10 Hz - 100 MHz)

< 0.2% < 0.2% < 0.2%

Linewidth < 1 MHz < 1 MHz < 1 MHz

M2 <1.2 <1.2 <1.2

Beam Mode TEM00 TEM00 TEM00

Beam Diameter (at window, typ.)

0.2 mm 0.7 mm 0.2 mm 1 mm 0.2 mm 0.7 mm

Beam Divergence < 10 mrad < 1.5 mrad < 10 mrad < 2 mrad < 10 mrad < 2 mrad

Polarization 100:1, Linear 100:1, Linear 100:1, Linear

Circularity > 0.85 > 0.85 > 0.85

Length 50 mm 80 mm 50 mm 80 mm 50 mm 80 mm

Width x Height 44 mm x 25 mm 44 mm x 25 mm 44 mm x 25 mm

SLIM laser head SLIM laser head and OEM driver Collimated SLIM laser head and CE driver

www.laser2000.de 51

DPSS Lasers

CW DPSS lasers from Ultraviolet to Infrared A wide variety of continious wave (CW) lasers is available

at the following wavelengths: 355 nm, 375 nm, 405 nm, 430 nm, 440 nm, 457 nm, 473 nm, 501 nm, 523 nm, 532 nm, 543 nm, 556 nm, 561 nm, 589 nm, 593 nm, 612 nm, 633 nm, 635 nm, 671 nm, 690 nm and others.

For full information use the Laser 2000 Web Site www.laser2000.de

Pulsed UV Lasers for Molecular Unca-ging, Microdissection, MALDI TOF and Bio-Particle DetectionThe fast growing fields of bio-science are now taking advan-tage of the latest developments in DPSS laser technology. The Model DPS-3507-50 offers the precise pulse duration, power stability and UV wavelength parameters needed for photoli-sys of caged compounds. Molecular uncaging through UV photolisys is providing new avenues of understanding into neurological disorders and drug delivery methods.

Diode pumped solid state lasers for microscopes

LCL-GARNET laser for MALDI TOF, Laser microdissection

DPS-3507-50 complete laser system for Molecular Uncaging, Bio-Particle Detection

Model nameWavelength

(nm)Pulse Energy (µJ at 1 kHz)

Beam diameter

(mm)

Beam divergence (half

angle, mrad)Polarization

LCL-LCS-DTL-378QT 351 40 < 1 < 5>100:1,

horizontal

LCL-LCS-DTL-374QT 355 20 < 1 < 5>100:1,

horizontal

LCL-GARNET 355 20 0.4 < 1.7>100:1,

horizontal

Specifications DPS-Series 3500

Power at 355nm 0.1W - 2W

Pulse Repetition Rate 20 - 150 kHz

Pulse width (nominal) 20 - 100 nsec

Wavelength 354.7 nm

Mode (M²) Specification TEMoo(<1.25)

Beam Diameter (1/e²) 1.5 mm

Pulse to Pulse Stability < 10%

Power Stability (8 hr. drift at constant temp.) <5%

Beam Pointing Stability at constant temp. <50 urad

Polarization (Linear, Vertical) >100:1

Beam Divergence (full angle) < 0.5 mrad

Input voltage 90-240 VAC

Power consumption, maximum 500 W

Ambient operating temperature (non-condensing) 10-30 °C

Laser head dimensions (LWH) 20.0 x 7.5 x 6.5 in.

Laser head weight 30 lbs.

Laser power supply dimensions (LWH) 12.2 x 13.5 x 5.4 in.

Product SpecialistsBernhard Dauner+49 8153 [email protected]

Dr. Stefan Kremser+49 (0) [email protected]


52 Call us: +49 (0)8153 405-0

Deep UV Gas Lasers

Deep UV Gas Lasers

Deep UV Gas Lasers

Deep UV Gas Lasers

Series 30 laser with integrated controller

Series 70 laser with integrated controller

224 and 248 nm

Wavelengths 224 nm, 248 nm

Narrow line width <0.5 pm (3 GHz, 0.1 cm-1)

Innovative plug- and play instrument solutions

Rugged design for reliability

USB or Ethernet interface with LabView

Built-in laser power monitor

Wide operating environment (-200 – 100 °C)

Instant on (< 10 µs from cold start)

90 VAC – 240 VAC input at < 10 W, no water cooling or toxic chemicals

Deep ultraviolet for less

The DUV family of lasers offer 224.3 nm and 248.6 nm for frac-tion of the cost of the competition. The laser is the size, weight and power consumption of a HeNe laser but with output in the deep UV. The self-contained, integrated, laser controller enables remote computer control for ease of operation and flexible data collection via LabView software. With an input power less than 10 W the need for water cooling and other thermal management issues is eliminated. The lasers reach full power in less than 20 microseconds from a cold start from any ambient temperature from -200 to 100 °C without prehea-ting or temperature regulation. With output over 100mW and linewidths less than 3GHz or 0.0005nm these are great sources for a wide range of applications.

Ultra-easy ultravioletMake ultra-sensitive measurements of Raleigh, Raman, fluo-rescence or phosphorescence emissions generated by deep UV excitation. Our “instrument solutions” combine a deep UV laser source with an array of analyzer and detector plug-and-play modules. Detection choices include single and multi-channel PMT and photodiode detector modules that are gated in synchronism with the laser and offer flexible boxcar inte-gration and averaging for enhanced signal-to-noise data coll-ection. Analyzer options include UV Raman, laser induced na-tive fluorescence, CE and HPLC modules. The combination of plug-and-play source, analyzer and detector modules enables you to rapidly develop breadboard and prototype instruments for a wide range of applications from research to product ana-lysis to environmental monitoring with data sampling rates up to 20 Hz.

Flexible for the lab, made for the real worldAn array of accessories such as emission line purity modules and fiberoptic couplers enable you to mate our componen-ts with a wide range of devices from third-party suppliers. Communication with the laser and all plug-and-play modules is accomplished via USB or Ethernet using LabView drivers. Our „instrument solutions” provide a seamless fit for many applications such as laser induced native fluorescence or UV resonance Raman analyzers, photoluminescence, capil-lary electrophoresis, high performance liquid chromatogra-phy, phosphorescence and many other types of instruments. Rugged design, reliable performance and low cost make them ideal for the field researcher and the OEM.

Deep UV laser, plasma line filter, cuvette holder, PMT adapter and 1/2” filter adapter all interconnect with standard optics and Hama-matsu PMTs to enable a complete system

Deep UV Gas Lasers

www.laser2000.de 53

Deep UV Gas Lasers

HeAg lasers @ 224.3 nm

Model PSY-HeAg70-224SL PSY-HeAg30-224SL

Peak power(quasi cw) > 50 mW > 10 mW

System dimensions 10 cm x 10 cm x 70 cm 5 cm x 13 cm x 30 cm

System weight 3.6 kg 1.4 kg

Pulse frequency 0 - 20 Hz 0 - 5 Hz

Longitudinal mode spacing 257 MHz 642 MHz

Pulse width 20 - 120 µs, adjustable

Pulse synchronism internal or external

Beam diameter 3 mm

Beam divergence < 4 mrad

Oscillation bandwidth < 0.5 pm, < 3 GHz, < 0.10 cm -1

Power consumption < 10 W

Line requirements 90 - 250 VAC, 47 - 63 Hz oder 24 VDC

NeCu lasers @ 248.6 nm

Model PSY-NeCu70-248SL PSY-NeCu30-248SL

Peak power(quasi cw) > 250 mW > 50 mW

System dimensions 10 cm x 10 cm x 70 cm 5 cm x 13 cm x 30 cm

System weight 3.6 kg 1.4 kg

Pulse frequency 0 - 20 Hz 0 -5 Hz

Longitudinal mode spacing 257 MHz 642 MHz

Pulse width 20 - 80 µs, adjustable

Pulse synchronism internal or external

Beam diameter 3 mm

Beam divergence < 4 mrad

Oscillation bandwidth < 0.5 pm, < 3 GHz, < 0.10 cm -1

Power consumption < 10 W

Line requirements 90 - 250 VAC, 47 - 63 Hz oder 24 VDC

Digital Detector Controllers

Digital Detector Controller

The tiny „instruments on a chip” are key to the Deep UV laser systems. They include multichannel digital photo multiplier tube controllers with gated boxcar integrators. This accessory is a direct plug-in unit to the lasers and enables ultra-sensi-tive detection simultaneously in ten or more channels auto-matically synchronized with the deep UV laser. We also offer plasma line cleaning optics modules, fiber optic interfaces and many more accessories. Together they form a catalog of op-tions from which a researcher can choose to develop his own tools for sensitive instrument.

Laser Line Filter System (PLRFS) for Deep UV Laser

Digital Detector Controller

Product SpecialistsDr. Stefan Kremser+49 (0) [email protected]

Bernhard Dauner+49 8153 [email protected]


54 Call us: +49 (0)8153 405-0

Photon Counting

Spectroscopy Cameras

SensL, Lee House, Riverview Business Park, Bessboro Road, Blackrock, Cork, IrelandTel. +353 214 350 442, Fax. +353 214 350 447, [email protected], www.SensL.com

PCDMiniMiniature Photon Counting Device

Version 1 Revision 3.0

Results taken refer to the PCDMini-s0020

Parameter Minimum Typical Maximum Units Notes

Spectral range1

400 950 nm

Active areaPCDMini-s0010PCDMini-s0020PCDMini-s1000

10201000

mmm Via lens attachment

Jitter2

120 130 140 ps

Time-walk3

50 ps 10kcps to 1Mcps

Dark count 3 80 100 cps Min dark count @ -20°C

Peak Quantum

Efficiency1

45 % Max QE @ -20°C,

550nm – 650nm

Peak afterpulsing4

0.14 0.30 %Output pulse

width5 53 ns

Output amplitude 2.5 V 50load

Dead time5

98 ns

Maximum count6

9 10 Mcps Continuous light

Supply voltageVCC

Quench resetQuench operationDetector Bias

101426

5

121528

131640

V

VVV

@50mA without cooling@200mA with cooling@10mA@10mADependent on APD breakdown voltage

Cooling time 10 s -20°C

Specifications

5. Output Pulse & Dead Time

Dead time

6. Maximum Count Rate

Wavelength (nm)

1. Quantum Efficiency

400 1000

45

600 800

QE (%)30

15

0

3. Time-walk

50ps

1Mcps

10kcps

20.0

Count(cps)

Time (ns)21.020.5

50k

0

Time (ns)21.0

2. Jitter50k

020.0 20.5

130psCount(cps)

1000Time (s)

4. Afterpulsing

1001010.1

0.15

0

Afterpulsing Probability (%)

SensL, Lee House, Riverview Business Park, Bessboro Road, Blackrock, Cork, IrelandTel. +353 214 350 442, Fax. +353 214 350 447, [email protected], www.SensL.com

PCDMiniMiniature Photon Counting Device

Version 1 Revision 3.0

Results taken refer to the PCDMini-s0020

Parameter Minimum Typical Maximum Units Notes

Spectral range1

400 950 nm

Active areaPCDMini-s0010PCDMini-s0020PCDMini-s1000

10201000

mmm Via lens attachment

Jitter2

120 130 140 ps

Time-walk3

50 ps 10kcps to 1Mcps

Dark count 3 80 100 cps Min dark count @ -20°C

Peak Quantum

Efficiency1

45 % Max QE @ -20°C,

550nm – 650nm

Peak afterpulsing4

0.14 0.30 %Output pulse

width5 53 ns

Output amplitude 2.5 V 50load

Dead time5

98 ns

Maximum count6

9 10 Mcps Continuous light

Supply voltageVCC

Quench resetQuench operationDetector Bias

101426

5

121528

131640

V

VVV

@50mA without cooling@200mA with cooling@10mA@10mADependent on APD breakdown voltage

Cooling time 10 s -20°C

Specifications

5. Output Pulse & Dead Time

Dead time

6. Maximum Count Rate

Wavelength (nm)

1. Quantum Efficiency

400 1000

45

600 800

QE (%)30

15

0

3. Time-walk

50ps

1Mcps

10kcps

20.0

Count(cps)

Time (ns)21.020.5

50k

0

Time (ns)21.0

2. Jitter50k

020.0 20.5

130psCount(cps)

1000Time (s)

4. Afterpulsing

1001010.1

0.15

0

Afterpulsing Probability (%)

Silicon Solutions for Low Light DetectionWe offer a range of innovative silicon-based low-light imaging and photon counting sensors, arrays and modules. Products include photon-counting detectors and large-area high-gain APD.

APD in Geiger Mode

Large Area High Gain APD

Time binning 2.5 nanoseconds

Highest QE reached by back thinning

This unique silicon photon counter is based on a new shal-low junction Geiger-mode avalanche photodiode technology. SensL‘s products and services provide a revolutionary alter-native to the PMT Photomultiplier Tube, the existing standard for photon counting applications. Compatible and adaptable, SensL‘s products are ideal for applications such as biosensors, quantum cryptography, medical diagnostics, laboratory instru-mentation, environmental monitoring and space exploration.

Typical main markets and applications are:

Photon Correlation Spectroscopy

Confocal Microscopy

Fluorescence Lifetime Measurement

Biological Sensors

Microarray Scanning

DNA Biochips/Sequencing

Bio/Chemical Sensors

Scientific Instrumentation

Proteomics/Protein Biochips

Flow Cytometry

Capillary Electrophoresis

Nuclear Medicine (PET/SPECT Scanning)

Product SpecialistDr. Helge Brüggemann+49 (0) 30 962 [email protected]

Sales Assistance Gabriela Thunig+49 (0) 8153-405-43 [email protected]

Photon counting

High gain sensing

www.laser2000.de 55

Spectroscopy Cameras

Key FeaturesHigh QE between 950-1650 nm

<1 e- rms read noise

16 bit dynamic range

1024 X 256 Pixel Format

26 µm pixel pitch

-40 °C TE Cooling

USB 2.0 data port

External controller NOT required The MOSIR™ 950 stands alone as the world‘s first and exclusive NIR image intensified spectroscopy camera between 950 and 1650 nm.

Based on Intevac‘s patented and exclusive transfer electron (TE) photocathode and electron bombarded (EB) gain techno-

logy, the MOSIR 950 is ideal for low light spectroscopy and imaging applications including Raman spectroscopy, chemical imaging, astronomy, and photoluminescence. The combinati-on of high QE and low noise gain between 950 and 1650 nm provides far superior sensitivity than CCD‘s or InGaAs arrays.

The MOSIR focal plane array has an ideal 4:1 aspect ratio spectroscopy format (1024X256, 26X26ìm pixel pitch), 16 bit dynamic range, USB 2.0 data port and interfaces to leading spectrometers and microscopes. Dark charge is minimized for all sensors with a proprietary thermo-electric cooling and va-cuum design. The MOSIR camera is extremely compact and does not require an external controller.

900

Sensor fomat 1024 x 256 imaging pixels; 26 x 26-um pixels; 100% fill factor; 26.6 x 6.7-mm imaging area

System read noise e- rms @ scan rate Minimum Typical Maximum

100 kHz 7 10

1 MHz 17 20

2 MHz 25 30

Spectrometric well capacity

single pixel 250 ke- 500 ke-

binned 600 ke- 1 Me-

Photocathode Dark current e-/pixel/sec @ -40C

-40 deg C operation 7k 18k

Minimum Photocathode Cooling Temperature -40 deg. C -50 deg. C

Spectral Response see QE curve

EB Gain 200

Excessive Noise Factor 1.1

Minimum Gate Width 10 usec

Maximum Repetition Rate 30 Hz

Software-selectable gains 4 e-/count, 8 e-/count, 16 e-/count,

Dynamic range 16 bits

Nonlinearity <2%

Spectral rate, Hz, full vertical binning

100 kHz digitization 50

1 MHz digitization 180

Sensor cooling range +20 deg C to -40 deg C

Temperature stability +/-0.1 deg C over entire temperature range;

Ambient Operating Temperature 0 deg C to +30 deg C

INTEVAC, INC. 3560 Bassett Street Santa Clara, CA 95054-2704Phone 408-588-2150 Fax 408-727-5739 www.mosir950.com/NOTE: This product is under the export control of the Office of Defense Trade Controls, US Department of State, and is subject to theInternational Traffic in Arms Regulations. Transshipment to any destination outside the United States without the knowledge and consent of theOffice of Defense Trade Controls is strictly prohibited.

The information and specifications included in this datasheet are preliminary and subject to change.

MOSIRTM 950 SPECIFICATIONS

900

Sensor fomat 1024 x 256 imaging pixels; 26 x 26-um pixels; 100% fill factor; 26.6 x 6.7-mm imaging area


100 kHz 7 10

1 MHz 17 20

2 MHz 25 30




Photocathode Dark current e-/pixel/sec @ -40C

-40 deg C operation 7k 18k

Minimum Photocathode Cooling Temperature -40 deg. C -50 deg. C

Spectral Response see QE curve

EB Gain 200

Excessive Noise Factor 1.1

Minimum Gate Width 10 usec

Maximum Repetition Rate 30 Hz



Nonlinearity <2%






Ambient Operating Temperature 0 deg C to +30 deg C

INTEVAC, INC. 3560 Bassett Street Santa Clara, CA 95054-2704Phone 408-588-2150 Fax 408-727-5739 www.mosir950.com/NOTE: This product is under the export control of the Office of Defense Trade Controls, US Department of State, and is subject to theInternational Traffic in Arms Regulations. Transshipment to any destination outside the United States without the knowledge and consent of theOffice of Defense Trade Controls is strictly prohibited.

The information and specifications included in this datasheet are preliminary and subject to change.

Typical QE Response for 30-11 (BBAR and UVAR)

0

10

20

30

40

50

60

70

80

90

200 300 400 500 600 700 800 900 1000 1100

Wavelength (nm)

Qu

antu

m E

ffic

ien

cy (

%)

30-11-BBAR30-11-UVAR

CCD Image sensor Marconi CCD30-11; scientific grade 1; AIMO; MPP back illuminated, anti-reflection coating

CCD fomat 1024 x 256 imaging pixels; 26 x 26-um pixels; 100% fill factor; 26.6 x 6.7-mm imaging area


100 kHz 7 10

1 MHz 17 20

2 MHz 25 30




Dark current e-/p/hr

-40 deg C operation 7 18

Maximum Cooling Temperature

TE Cooling -60 -70



Nonlinearity <2%

Vertical shift time 30 us






INTEVAC, INC. 3560 Bassett Street Santa Clara, CA 95054-2704Phone 408-588-2150 Fax 408-727-5739 www.mosir950.com/The information and specifications included in this datasheet are preliminary and subject to change.

MOSIRTM 950 TE Photocathode QE Curve MOSIRTM 350/150 QE Curve

UV/VIS/NIR Image intensified Spectroscopy Cameras

5� Call us: +49 (0)8153 405-0

Spectrometer Spectrometer

NIR InGaAs Spectrometers

Our high performance NIR InGaAs spectrometers use a linear photo diode array detector with 512 pixels (1024 optional) to provide maximum sensitivity. The NIR InGaAs spec-trometers use single strand SMA-905 fiber optic input. Several models provide a variety of operational ranges and resolutions suitable for both spectroscopy and op-tical spectrum analysis. NIR applica-tions include chemical ID and moisture analysis, SpectroRadiometry and op-tical power measurements, laser cha-racterization, microsensor applications, and thicker thin-film measurements.

Dynamic Range: 4000:1 with 5 decades Dimensions: 150 x 100 x 70 mm

Optical Resolution: up to < 1 nm resolving res. with 25um slit Power Consumption: 2 A @ 5 VDC

Detector Type: 512 or 1024 pixel InGaAs PDA Interface: USB-2 or parallel

Detector Range: 900 - 1700 nm Data Transfer Speed: 40x faster than USB-1

Pixel Size: 25 µm x 500 µm Detector Integration: 1 ms to 30 s

Signal to Noise: 4000:1 with TEC cooling Slit Size Options: 25, 50, 100, or 200 µm

Digitizer: 14-bit @ 2.5 MHz rate Fiber Optic Input: SMA-905 0.22 NA single fiber

Operating Systems: Win 9x/NT/00/XP

Software Included: SpectraWiz program & apps

Also free programs for: LabView / VC / VBA / Delphi

14390 Carlson Circle, Tampa, Florida, 33626 USA www.StellarNet-Inc.com Voice: +1-813-855-8687 Fax: +1-813-855-2279 [email protected]

www.StellarNet-Inc.com

Analytical Instrumentation Surf the New Wave in Portable Fiber Optic Spectrometry

EPP2000-NIR-InGaAs Spectrometer - for Near Infrared Applications

StellarNets’ high performance InGaAs spectrometers cover the NIR wavelength range from 0.9-2.2um. The units are exceptionally robust with no moving parts and are packaged in small rugged metal enclosures (2.75 x4 x6 inch) for portable, process, and lab applications. The InGaAs detector is a Sensors Unlimited linear photo diode array with 512 pixels (1024 optional) 25um by 500um tall to provide maximum sensitivity. The detector has an integrated thermo electric cooler (TEC) maintained at –10 C, stabilized within +/-0.1 C. The EPP2000-NIR-InGaAs spectrometers

use single strand SMA 905 fiber optic input. Several models provide a variety of operational ranges and resolutions suitable for both spectroscopy and optical spectrum analysis. NIR applications include chemical ID and

moisture analysis, SpectroRadiometry and optical power measurements, laser characterization, microsensor applications, and thicker thin-film measurements.

The SpectraWiz software is included for Win9x/Me/NT/2000/XP to accurately measure wavelength emissions, reflectance, transmission, absorption, and absolute intensities. Driver and customizable programs are also included for operation in LabVIEW, Excel+VBA, VC, and Delphi.

The system includes high speed plug & play interfaces for notebook and desktop com-puters. The USB2EPP cable connects to USB-2 ports (40x faster than USB1).

Specifications Zero defect 512 detector EPP2000-NIR-InGaAs Spectrometer $13,125 Dynamic range: 4000:1 with 5 decades Dimensions: 150 x 100 x 68.8 mm Resolving resolution: 3.1nm with 25um slit Power consumption: 2 Amps @ 5 VDC InGaAs Detector: 512 pixel cooled PDA array Interface: USB-2 and Parallel Detector range: 0.9-1.7um (900-1700nm) Data transfer speed: 40x faster than USB-1 Pixel size: 25um x 500um Detector Integration: 1 millisecond to 30 secs Pixel well depth: 130 x10^8 electrons Slit size options: 25, 50,100, or 200um Selectable well control: 130 x10^8 or 5 x10^6 el. Operating systems: Win98/NT/Me/00/XP Signal to noise: 4000:1 with TEC cooling Software included: SpectraWiz program & apps Digitizer: 14 bit @ 2.5 MHz rate Also free programs for: LabView,Excel+VBA,Delphi

The SpectraWiz software is included to accurately measure wavelength emis-sions, reflectance, transmission, ab-sorption, concentrations, and absolute intensities.




Spectrometer

UV-VIS Spectrometers

The EPP2000-C-UV/VIS spectrometers utilize a 40 mm dia-meter concave grating with aberration correction to provide superb imaging. This significantly improves spectral shapes by eliminating coma and stigmatism found in plane grating spectrograph designs. The flat field spectrograph architecture does not utilize mirrors, and therefore provides the lowest possible stray light in the UV with additional Assistance from the holographic line grating. A multi-band filter is integrated into the spectrograph to provide order sorting and prevent op-tical aliasing. The optical signal is input via single strand fiber optic cable into a standard SMA-905 connector.

The SpectraWiz software is included to accurately measure wavelength emissions, reflectance, transmission, absorption, concentrations, and absolute intensities.

Dynamic Range: 2000:1 with 6 decades Dimensions: 150 x 100 x 70 mm

Optical Resolution: < 1 nm Resolving Res. with 25um slit Power Consumption: 100 mA @ 5 VDC

Detector Type: 2048 pixel CCD, PDA opt. Interface: USB-2 or parallel

Detector Range: 190 - 850 nm or 250 - 885 nm Data Transfer Speed: 3x / 40x faster than USB-1

Pixel Size: 14 µm x 200 µm Detector Integration: 2 ms / 1 ms [12 / 14-bit] to 65 s

Concave Grating: Aberration corrected Slit Size Options: 5, 10, 25, 50, 100, or 200 um

Grating Type: Holographic, 590 g/mm Stray Light: 0.02% @ 435 nm; 0.2% @ 200 nm

Spectrograph: f/2, Flat field - No mirrors Fiber Optic Input: SMA-905 0.22 NA single fiber

Order Sorting Filter: Integrated multi-band Operating Systems: Win 9x/NT/00/XP

Signal to Noise: 1000:1 CCD, PDA 2000:1 Software Included: SpectraWiz program & apps

Digitizer: 12-bit, 14-bit optional Also free programs for: LabView / VC / VBA / Delphi



58 Call us: +49 (0)8153 405-0

Spectrally Programmable Light Engine

Broad band light source

If you could instantly control the spectrum, intensity and tim-ing of your light source, what could you achieve?

Replacing light created by a frustrating assemblage of me-chanical devices, OneLight Spectra improves illumination sys-tems using a patented software-driven light engine – or Digital Light Source – to achieve an entirely new category of illumina-tion. Employing simple-yet-powerful software, precise spec-tral profiles are created. Illumination, intensity and exposure duration are under your complete control. And the result is nothing short of evolutionary.

THE EVOLUTION OF SPECTRAL CONTROL SPLE technology marks a major departure from conventional light solutions. It allows for any desired mix of wavelengths (color) and intensity of illumination to be dynamically config-ured and instantly selected under software control to produce currently unattainable contrast, intensity, purity and range of color.

Able to respond faster than most cameras capture images, OneLight Spectra can improve the performance of most mi-croscopy or spectroscopy imaging systems. Fully program-mable, the range of wavelengths you can use is no longer de-fined by how many band-pass or neutral density filters your filter wheel can accommodate, or how fast you can change them. Wavelength requirements are defined using an intuitive software application, instead of the coating laboratory. In this sense, OneLight Spectra is like having a filter factory right at your fingertips, with none of the limitations.

SOFTWARE CONTROL

OneLight Spectra comes with an intuitive software interface that provides easy command of the most commonly used func-tions of the instrument. Users can set up and save their own particular instrument configurations; recalling them quickly and easily to continue an experiment, without having to repeat the setup procedure. You can save and load any spectral pro-files you create, as well as those from the libraries of spectral profiles that are provided to get you started.

The software is organized around a modular plug-in architec-ture, making it easy to add new features by simply adding new plug-ins. For example, the Tuner plug-in provides selection of wavelength bands or “filter functions” that are the digital light equivalent to short-pass, long-pass, band-pass or notch filters, and are just a few mouse clicks away.

Freeform is another plug-in that provides a graphic equalizer-type application for the construction of arbitrary spectral pro-files on the fly. You can also construct spectral profiles from data you have measured, created or captured in any other manner. For those who want to add functionality and develop software for their own applications, we provide an optional SDK that gives programmatic access to all the functions avail-able.

Simply put, creating light has never been this easy. And once you have experienced the simplicity and flexibility with which you can create light using OneLight Spectra’s software, you will never want to do it any other way.

www.laser2000.de 59

Broad band light source



Deep UV Gas Lasers

Optics and Optomechanics

The full range of available products is offered...

Optomechanics:optical mounts

stages (translation, rotation, goniometer)

motorized mounts and stages

mounting systems

OEM solutions

Optics:a wide variety of optics (mirror, filter, lenses, etc.)

optical coatings

Optics & Optomechanics

�0 Call us: +49 (0)8153 405-0

Laser Safety GogglesFull spectrum Polycarbonate laser protection

Laser goggles are provided by companies with more than 30 years experience in the field of eye safety for medical and industrial applications. Filters for wavelength regions between the UV to IR (190 nm-1700 nm) are available and pro-tect against laser powers up to 100W (cw). Also goggles for short pulse lasers as femtosecond- or picosecond lasers are certified.

The light and very robust material together with a variety of frames allows the user to find the right goggle for comfortable usage.

For full information use the Laser 2000 Website (www.laser2000.de). All laser goggles shown are fully CE and EN207 certified.

As registered user you will also be able to download a program to easily identify the necessary filter for your application. If you still have questions please contact our product specialists.

Please contact Victoria Benedikt for catalogues:+49 (0) 8153-405-61 [email protected]


Dr. Andreas Stangassinger+49 (0) [email protected]


Isabell Langfellner+49 (0) 8153-405-26 [email protected]

3001Webcode:

Deep UV Gas Lasers

www.laser2000.de �1

c Please keep me informed by eMail. I am particularly interested in:c Fiber Opticsc Network Technologyc Digital Testingc Lasers & Lightsources

c Ophthalmic Solutions c Laserprotection c Optics & Optomechanicsc UV Technology

c Infrared Technology c Optical Instrumentation c Image Processing

Free catalogues!Mail order for your catalogFax +49 8153 405-33c Fiber Optics, 240 pages, in German

c Network Solutions, 16 pages, in German

c FO Components & Lab Equipment, 40 pages

c Optical Filters for Life Sciences, 62 pages

c Lasers and Lightsources, 124 pages, in German

c Optical Instrumentation, 104 pages, in German

c Laser Safety, 24 pages, in German

c Image Processing, 72 pages, in German

c Infrared Technology, 48 pages, in German

c Ophthalmic Systems, 12 pages

c Call me back please.

Optical Filters_Bulletin No. 3011

Company / University, Department

Last name, First name Title

Street Number

Zip-Code / City Country

Phone with prefix eMail adress

Find all these products and many more on our web page www.laser2000.com:• Fiber Optics

• Network Technology

• Digital Testing

• Lasers & Lightsources

• Ophthalmic Solutions

• Laserprotection

• Optics & Optomechanics

• UV Technology

• Infrared Technology

• Optical Instrumentation

• Image Processing

9001Webcode:

www.laser2000.comAs part of our continuous program for product improvement, Laser 2000 reserves the right to change specifications without notice. Copyright © 2009 Laser 2000 GmbH. All Trademarks are the registered property of their respective owners.

U.K. Phone +44 1933 461666 Fax +44 1933 461699 [email protected] Phone +32 71 610 640 Fax +32 71 610 649 [email protected] Phone +31 297 266 191 Fax +31 297 266 134 [email protected] Phone +46 11 369 681 Fax +46 11 369 689 [email protected]

Office BerlinPasedagplatz 3-4DE-13088 BerlinTel. +49 30 962778-0Fax +49 30 [email protected]

Office DresdenGeschwister-Scholl-Str. 47DE-01877 BischofswerdaTel. +49 3594 705980Fax +49 3594 [email protected]

Office MönchengladbachOhlerkamp 4 DE-41069 MönchengladbachTel. +49 2161 307300Fax +49 2161 [email protected]

Office BambergHängbergstrasse 18DE-96199 ZapfendorfTel. +49 9547 870369Fax +49 9547 [email protected]

Office WienChristian SchöbelAT-1160 WienTel. +43 1 4810498Fax +43 1 [email protected]

Laser 2000 GmbH Argelsrieder Feld 14 DE-82234 Wessling Munich/Germany

Tel. +49 8153 405-0 Fax +49 8153 405-33 [email protected]

Find all these products and many more on our web page:

• Fiber Optics

• Network Technology

• Digital Testing

• Lasers & Lightsources

• Ophthalmic Solutions

• Laserprotection

• Optics & Optomechanics

• UV Technology

• Infrared Technology

• Optical Instrumentation

• Image Processing

www.laser2000.com

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