Sterilization and Disinfection

Groups 1:Nadhira Adelina S.(130210103005)Solikha (130210103007)Candra Pratama H.(130210103031)Rini(130210103096)

Sterilization is method to kill all of the microorganism,so something (tools, material, media, etc ) is sterile.

Disinfection is a method to kill a microorganism,expecialy the organism is cause disease.

The Principles of Sterilization

mechanical physical chemical

mikrofilter warming irradiation

Pressure chemicals

It use for materials not resistant warming or the method dont do something because condition .Example : Formaldehyda, hibitane, Cidex. Sterilization with use a big tool material : HCl, HgCl2, Alkohol, Formalin, Phenol, ChlorinStep by step :Washing the tool with water and detergentWith HCl : Soak in HCL 10% for 2 daysWith Chlorin : Soak in chlorin liquid 150 mg/l for 12 24 hoursNetralisitation with Na-Thiosulfat 40 50 mg/lRinse with water until the smell is loseSterilization in environment antiseptik, UVSterilization Mediawater and chlorin will be netralisitation with Na-Thiosulfat

7agentThe mechanism of actionExample surfactantDamaging membraneSoap ,detergentsA cationic detergentProtein in denaturing, damage fatAntiseptic , disinfectant , benzal koniun chlorideAn acid and an organic basePh high or lowInhibitor fungi , an example of benzoic or soda alcoholProtein in denaturing, damage fat,and cause dehidration microorganismAn antiseptic and disinfectant: ethanol and of isopropylfenolicdamageto the cell membranedisinfectantaldehids denaturate proteinGluteraldehida formaldehyde (disinfectan)Etilen oksida denaturate protein-oxidation denaturate proteinHidrogen peroksida, benzoil peroksidaThe materialAlcohol is a substance that most effective and can make for sterilization and disinfectionThe method Soaking sterilization Gas sterilization Hidrogen Peroksida Ethylen Oksida Aldehida (Formalin)

Cara Dingin (Perendamam)

91. Warminga. Glowing (with fire directly) to burn the tools with fire directly example the tools is needle, pincers, etcb. Hot and dry fumigation of meat for tool from glass, such as : fleaker, test tube, etcc. Steam Boiling hot vapor the concept same with steaming. for the tool from material has a gynaecology water, so wont there are dehidration.d. Autoclave hot vapor autoclave2. Irradiating uv raysexample: kill the microba in interior Safety Cabinet3. Pressureexample: Autoclave

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When the source of heat is turned, Autoclave water in increasingly about to boil over.Water vapor formed would urge air that fill autoclave.A valve steam / air closed so that the pressure of the air in autoclave up.At the time of achieved pressure and a suitable temperature , the sterilization process is started and the timer starts to count time backwards.After process of sterilizing finished, the source of heat is turned off and pressure a descent be left until it reaches 0 pounds.Autoclave cant be opened before the pressure reaches 0 pounds.

agentThe mechanism of actiondescriptionSteamBoiling hot vaporIn denaturing proteinKill bacteria in vegetative, an endospore virus live 100 CAutoclave hot vapor

In denaturing proteinKill all microbesPasteurization hot vapor

In denaturing proteinKill pathogens in food products , 63oc , 30 seconds ( 72oc , 15 seconds ) ( 140oc , 3 second )Steam heat, oven hot water

Oxidation and in denaturing protein170OC, 2 jam, for sterilization tools and glass

osmotic pressure the addition of a salt or sugar

Plasmolisis contaminants

Used for preservation food, less effective to fungiUv radiation

Damage the dna

Radiation of x raysDamage the dnaFor sterilizing medical equipment

Filtration, microfilter : 2,5 - 3 / 0,22 - 0,45 The principle : liquid through a strainer porous (suppressed by centrifugation style or vacuum pump). Bacteria restrained in a sieve but viruses didnt filter.Condition :Sterile venues being used as a container.Filtered using media microfilter (2,5 - 3) and directly on the receptacle placed sterile.Closed meeting with aluminum foil.Performed in laminar air flow

Non-disposable filtration apparatusDisposable filter cup unitSyringe filtersSpin filters

Non-disposable filtration apparatus

Disposable filter cup unit

Syringe filtersSpin filters

The mechanism of using non-disposable filtration apparatus1. Sterilisation the membranes (sieve erlenmeyer collectors and paper).2. To put in a barge (aseptic of the equipment, as a picture and the contents of the discharge of a funnel will be sterilized.3. Connect with vacuum pumps erlenmeyer valve and then turn on the engines.4. After all the discharge of the membrane and filter erlenmeyer be accommodated, the collectors of the discharge of may be moved to another which is sterile and aluminum foil or cotton cap with a sterile.Disinfection is to kill a disease-causing microorganism with chemicals or physically , this could reduce a possible infection with pathogens kill microorganisms .

Disinfectant is a chemical substance that is worn for disinfection

Distrub the function of cell membranDistrub the function of proteinAlkilasi reactionBind sulfhidril groupOksidationMutation , adaptation, resistantion, and effect of toxic.

Disinfection damage cell membran of bacteria, example: klorheksidin, senyawa amonium kuartener, alcohol and fenolFicsation cell membran,it cause koagulation so there are mechanism of formaldehid and glutaraldehid.Oksidation material, example halogen group,such as hipoklorit, it is more active than bromida.

1. Before opening room in erlenmeyer , should be part of the mouth of this instrument is passed fire beforehand2. Tweezers , stems l , etc can be sprayed with alcohol beforehand and burned .3. The tips of needles inokulum which has been heated must be awaited first cold or it can be affixed to cover the cup in part to accelerate the transfer of heat that occurred.4. An instrument which is expected to work for the sterile conditions close to the fire.5. If work in safety cabinet not have to wear the bunsen burner but if outside safety cabinet the more source of fire hence the more guaranteed aseptic conditions. 24

Sterilization of Work Chair25Move the Microorganism with Aseptis

Move the microorganism From Mug27

Move the Microorganism with Pipette28POUR MEDIA

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