Journal Review - The Emerging role of in vitro electrophysiological methods in CNS Safety...
-
Upload
blessing-umoudit -
Category
Science
-
view
10 -
download
1
Transcript of Journal Review - The Emerging role of in vitro electrophysiological methods in CNS Safety...
Presentedby
Blessing Umoudit
Outline
• Background• In vitro electrophysiological approaches• In vitro CNS tissue models for electrophysiology• Emerging Models used in CNS safety pharmacology studies• Personal review• Conclusion
Image source: http://azneuromod.com/central-nervous-system-may-contribute-to-dpn/
Background• Central Nervous System • The ICH S7A guidelines recognized the CNS as a central pillar within safety
pharmacology • CNS drugs possess high attrition rates due to adverse effects• CNS adverse effects are mediated predominately by off-target drug activity• Altered neuronal function and network communication
• In response • There is a growing need to perform more comprehensive CNS safety
testing prior to first-in-human trials
Background cont’d Ion channels
Play a fundamental role in inter and intracellular communication and neuronal excitability
Subjected to rigorous studies
Some off target interactions are associated with ion channels HERG, Voltage-gated sodium channels (5CN5A),
GABA type a receptor
In vitro Electrophysiological Approaches
• Patch Clamp technique• The “Gold standard”
• Clamping is by forming a giga-ohm seal between plasma membrane and a glass or quartz micropipette
• Used to measure biophysical and pharmacological activity of ion channels on millisecond timescales.
MANUAL AUTOMATIC
VersatileLacks the versatility and quality of manual technique
Yields low throughput High throughput
High maintenance cost and level of expertise
Inexpensive
Manual vs Automated Patch Clamp Technique
• Impalement Techniques• Forcible penetration of the membrane of large cells with sharp
micropipettes
• Xenopus laevis oocytes are commonly used• Common e.g. is two electrodes voltage clamping (TEVC)• In TEVC, one electrode acts as a dedicated membrane potential
sensor and the other as a current injector.
• Smaller electrodes mean less disruption in membrane and cytosolic ionic composition compared to patch clamp techniques
In vitro Electrophysiological Approaches Cont’d
In vitro Electrophysiological Approaches Cont’d
Common in vitro electrophysiological methods.
Expression systems
and immortalized
cell linesDissociated neuronal primary
cultures
3D - Neuronal Models
Brain slice models
In Vitro CNS Tissue Models for Electrophysiology
Incr
easi
ng co
mpl
exity
Expression Systems and Immortalized Cell Lines
• Are heterologous and recombinant expression systems(cells/cell lines) maintained in culture for an extended period of time.
• Non neuronal cells (e.g. Xenopus oocytes) were initially used
• Neural Stem Cells (NSC) are now being used• They possess Na+, K+ and Ca2 + currents in accordance with the known
patterns described for in vivo neuronal system
Dissociated Neuronal Primary Cultures
• Are neuronal cells mechanically and enzymatically dissociated from various brain regions
• Advantage• They retain most of their functionality in vitro• Success is dependent on specific requirement • This is dictated by the neurons cultured (e.g.
age of donor)
• Disadvantage• Cannot be maintained in culture for extended
periods and so would need to be freshly isolated regularly
Enzymatic dissociation of neuronal cells. (Hai-Yan Wu et.al 2014)
3D Neuronal Models• Consist of a co-mixture of different neuronal and non- neuronal sources• When grown in an in vitro 3D environment, neuronal cells demonstrate
better survivability as they are more similar to in vivo “3D” models.• Highly compatible with a number of electrophysiological techniques• Limitation• Cells within the centre of these models are exposed to less oxygen
and are often nutrient deprived
Brain Slice Models• Acute brain slice • Most accurate and the most common slice model used • Allows the use of intra and extracellular electrophysiological
techniques in a near in vivo situation• However, slice induced damage, lack of oxygen/glucose, bacterial
contamination limit the lifespan of these models
• Organotypic slice cultures• Capable of maintaining brain slice in culture on a stable substratum
over prolonged periods of time.• However, it does not retain its shape and thickness as it flattens to 3D
models
Animal Model Considerations• Rodents (mice and rats) are the primary species used in CNS
investigations• Transgenic mice enable the study of pharmacological drugs on
variety of conditions (e.g. Alzheimer’s disease, epilepsy etc.)• These models have shown good preclinical and safety testing
potential as they express the exact protein observed in the human situation
http://www.ddw-online.com/chemistry/p102797-zebrafish
https://en.wikipedia.org/wiki/African_clawed_frog
http://www.criver.com/products-services/basic-research/find-a-model/sprague-dawley-rat
Emerging Models in CNS safety pharmacology studies• Receptor Profile safety screening -Targets included on a recommended minimal panel for testing - Generates drug response profiles
• High-throughput techniques
• Liability Testing• Seizure liability assessment• Memory loss
Liability testing• Seizure LiabilityA number of pharmaceutical drugs targeting the CNS have been associated with seizures.
Case study : Minaprine an antidepressant withdrawn from market in 1996 due to an increase in clinical incidence of convulsions.
• Memory lossStudies have shown that compounds such as benzodiazepines that cause deficit in human memory have also led to dramatic changes in vitro.
Personal review• It is important to monitor these techniques to determine if
they are justifiable
• Suitability in preclinical studies (3RS)
• Predictive validity
Conclusion• Predictive power of in vitro models for CNS safety is low• The CNS is a complicated system and so there is need for
more enhanced means of studying it• Interspecies differences in ion-channel expression,
neuroanatomy and drug metabolism also reduce the predictive power in humans• Advancements have been made to techniques to make them
more applicable to in vivo conditions
Thank you!!!
Questions?