JOURNAL OF BACTERIOLOGYVOLUME 130 * NUMBER 3 * JUNE 1977

EDITORIAL BOARD

L. Leon Campbell, Editor-in-Chief (1980)University of Delaware, Newark

S. G. Bradley, Editor (1979)Virginia Commonwealth University, Richmond

Elizabeth McFall, Editor (1980)New York University, New York, N.Y.

Alien T. Phillips, Editor (1980)Pennsylvania State University,

University Park, Pa.

Carl Schnaitman, Editor (1978)University of Virginia, Charlottesville

Simon Silver, Editor (1981)Washington University, St. Louis, Mo.

Mark Achtman (1979)James N. Adams (1979)Arthur I. Aronson (1979)Gad Avigad (1977)Stephen D. Barbour (1979)Richard S. Berk (1977)Harriet Bernheimer (1977)Judith S. Bond (1977)Winfried Boos (1979)H. D. Braymer (1979)Jean Brenchley (1977)George H. Brownell (1977)Richard 0. Burns (1977)Peter I. Chapman (1979)G. William Claus (1979)Don B. Clewell (1977)Stanely N. Cohen (1979)Ronald S. Cole (1977)S. F. Conti (1978)Stephen Cooper (1978)John E. Cronan, Jr. (1977)Walter J. Dobrogosz (1979)Patrick R. Dugan (1977)A. Eisenstark (1979)Alan D. Elbein (1979)Wolfgang Epstein (1978)David P. Fan (1978)Walton L. Fangman (1978)

Gerad R. Fink (1978)W. R. Finnerty (1979)Harry E. Gilleland, Jr. (1979)Walter R. Guild (1978)Tadayo Hashimoto (1979)Gerald L. Hazelbauer (1978)George Hegeman (1977)James A. Hoch (1977)Joy Hochstadt (1977)Stanley C. Holt (1977)Philip Hylemon (1979)Joseph Inselburg (1978)Robert J. Kadner (1979)Sam Kaplan (1977)Jost Kemper (1978)David E. Kennel (1977)Bruce C. Kline (1977)Thomas G. Lessie (1979)Robert L. Lester (1978)Hillel S. Levinson (1978)K. Brooks Low (1979)Calvin S. McLaughlin (1978)P. T. Magee (1977)Michael H. Malamy (1978)Manley Mandel (1978)Robert E. Marquis (1979)Neil H. Mendelson (1978)Gene E. Michaels (1977)

Eugene W. Nester (1978)Donald P. Nierlich (1977)John H. Nordin (1979)Michio Oishi (1977)Ronald H. Olsen (1978)Sunil Palchaudhari (1979)Charles Panos (1978)Leo Parks (1979)Martin Pato (1978)William S. Reznikoff (1979)Palmer Rogers (1978)Antonio H. Romano (1979)Robert Rownd (1977)Harold L. Sadoff (1977)Milton H. Saier, Jr. (1979)Gene A. Scarborough (1979)Irwin H. Segel (1979)Jane K. Setlow (1978)J. A. Shapiro (1979)Issar Smith (1978)Lucile Smith (1978)Dwight Stinnett (1979)Robert L. Switzer (1979)Bonnie M. Tyler (1978)James A. Wechsler (1978)David White (1977)Clifford A. Woolfolk (1979)Howard Zalkin (1979)

Robert A. Day, Managing EditorGiseila Poliock, Assistant Managing Editor

Linda I1ig, Production Editor1913 I St., N. W., Washington, D. C. 20006

EX OFFICIOHarlyn 0. Halvorson, President (1976-1977)

J. Mehsen Joseph, Secretary

The Journal of Bacteriology, a publication of the AmericanSociety for Microbiology, 1913 I St., N.W., Washington, D.C.20006, is devoted to the advancement and dissemination offundamental knowledge concerning bacteria and other micro-organisms. The Journal is published monthly, and the twelvenumbers are divided into four volumes per year. The non-member subscription price is $105 per year. Single copies are$9.' The member subscription price is $21 per year. Corre-spondence relating to subscriptions, reprints, defective copies,availability of back issues, lost or late proofs, disposition of

A. Fredeick Rsmussen, Jr., Vice-President (1976-1977)Brinton M. Miller, Treasurer

submitted manuscripts, and general editorial matters shouldbe directed to the ASM Publications Office, 1913 I St., N.W.,Washington, D.C. 20006 (area 202 833-9680).

Second class postage paid at Washington, D. C. 20006, and atadditional mailing offices.Made in the United States of America.Copyright C) 1977, American Society for Microbiology.All Rights Reserved.

Author IndexAbdelal, Ahmed T. H., 1125Ahearn, Donald G., 1125Arias, A., 1139

Barker, Susan L., 1017Bayer, Manfred E., 1364Bayley, Susan A., 1274Beck, Barbara D., 1292Bloom, Fedric R., 983Bowers, Blair, 1357Bremer, H., 1098, 1109, 1206Broda, Paul, 1274

Callister, Heather, 1030Celis, T. F. R.; 1234, 1244Churchward, Gordon, 1206Cleveland, P., 1333Cooper, Terrance G., 1253Cox, B. S., 1303

Delisle, Gloria J., 1390Demain, A. L., 1010Downard, J. S., 1175Duggleby, Clive J., 1274Duval-Iflah, Yvonne, 1281

Finch, Lloyd R., 1047Fitzgerald, T. J., 1333Frazier, Marsha L., 1064Friebel, T. E., 1010Friedman, Stanley A., 991Fukuda, Akio, 1199

Gardiol, A., 1139Gibson, D. T., 1117Giles, Norman H., 1192Graham, Amy C., 1214Guest, John R., 1038

Hall, Ruth M., 973Harmon, Jeffrey M., 1224Hays, John B., 991Hohman, R. J., 1345Holm, C., 1303Holmlund, C. E., 1310

Jacobson, Eric S., 1397Jacobson, James W., 1192

Johnson, R. C., 1333Jones, Jay B., 1357, 1404

Kashket, Eva R., 1017Kennedy, Emily H., 1125Kennedy, Eugene P., 1072Kobayashi, G. S., 1387Kolenbrander, P. E., 1345Konisky, J., 1399Kumar, B. Vijay, 1387

Langworthy, Thomas A., 1326LeGall, Jean, 1084Leive, Loretta, 1364Le Mesurier, Sue, 1030Ljungdahl, Lars G., 1084Long, Richard A., 1159Lucas, M. Cran, 1192

Maas, Werner K., 1402McCready, S. J., 1303McKibbin, Janet, 1000McLaughlin, C. S., 1303McMillian, Ray, 1387Martin, W. G., 1159Martinez-Drets, G., 1139Mattick, John S., 973Medoff, Gerald, 1387Messenguy, Francine, 1253Metzenberg, Robert L., 1397Miller, J. N., 1333Mitchell, Alana, 1047Miwatani, Toshio, 1393

Ohnishi, Takeo, 1393Okada, Yoshimi, 1199Oliver, S. G., 1303Oxender, Dale L., 1024

Pace, Norman R., 1000Palchaudhuri, Sunil, 1402Park, James T., 1292

Quay, Steven C., 1024

Rahn, Shelley, 1402Raibaud, Pierre, 1281Rogers, J. E., 1117

Rousseau, Micheline, 1281Rownd, Robert H., 1262

Santos, Diogenes S., 1402Schenk, S. P., 1382Schlessinger, David, 1387Schneider, Henry, 1159Schroeder, Ellen, 1000Scott, William A., 1144Setlow, Barbara, 1130Setlow, Peter, 1130Shen, V., 1098, 1109Silverman, M., 1317Simon, M., 1317Singh, Ravendra P., 1130Snider, Martin D., 1072Sobus, M. T., 1310Sogin, Mitchell L., 1000Soucek, S., 1399Stadtman, Thressa C., 1357,

1404Stocker, B. A. D., 1214Streicher, Stanley L., 983Sutherland, P. A., 1303Sykes, J. A., 1333

Taber, Harry W., 1224Taichman, Lorne, 1262Takeda, Yoshifumi, 1393Tanaka, Yoshinori, 1393Tockman, Judith, 1091Tyler, Bonnie, 983

Van Heijenoort, Jean, 1281Vold, Barbara S., 1091

Wake, R. G., 1030Ward, J. Barrie, 1055Whittaker, N. F., 1310Williams, Peter A., 1149, 1274Woolfolk, C. A., 1175Worsey, Michael J., 1149, 1274Wyke, Anne W., 1055Wyss, Orville, 1382

Yang, Shiow-Shong, 1084Yoh, Myonsun, 1393

Zimmerman, Leonard N., 1064

INSTRUCTIONS TO AUTHORS

Submit manuscripts in duplicate (original andone copy) to ASM Publications Office, 1913 I St.,N.W., Washington, D.C. 20006. A $35 check ormoney order (non-refundable) must accompany themanuscript to cover handling costs. Institutionalpurchase orders will be accepted, but the author isrequested to utilize this procedure only if problemsare encountered in getting reimbursement from theauthor's institution. We solicit your cooperation inthis effort to hold down the cost of administration.General policy. Any manuscript submitted must

be a report of unpublished original research, whichis not being considered for publication elsewhere.Each manuscript should present the results ofan independent, cohesive study; numbered seriespapers are discouraged. When a submitted manu-script is judged to be more appropriate for the sub-ject scope of one of the other ASM journals, theEditors will transfer it to that journal for considera-tion, so notifying the author. In borderline cases,the preference ofthe author will be considered, butthe final decision rests with the respective Editorsand the Chairman of the ASM Publications Board.The "editorial style" of the Journal essentially

follows the CBE Style Manual (3rd ed., AIBS, 1972).For bacterial genetic systems, the recommendationsof Demerec et al. (Genetics 54:61, 1966) should befollowed, as updated and used by Bachmann et al.(Bacteriol. Rev. 40:116-167) for E. coli, Sanderson(Bacteriol. Rev. 36:558-586) for Salmonella, andLow (Bacteriol. Rev. 36:587-607) for F prime factors.For plasmids and plasmid-s ecified activities, ge-netic symbols should follow 4ovick et al. (Bacteriol.Rev. 40:168-189). For yeast, Chlamydomonas, andseveral fungal species, symbols such as those giveninHandbook ofMicrobiology (1974, A. I. Laskin andH. A. Lechevalier, eds., CRC Press, Cleveland, p.665-711, Vol. IV) should be employed. For geneticnomenclature, use italics consistently for genotypesymbols. Greater leniency can be allowed for pheno-typic symbols. However, they should not be itali-cized. The preferred convention is to use the samethree-letter symbols in Roman type with the firstletter capitalized.

Biochemical nomenclature, including abbrevia-tions and symbols, should follow the recommenda-tions of the IUPAC-IUB Commission on Biochemi-cal Nomenclature (CBN) and the Instructions toAuthors of J. Biol. Chem. and Arch. Biochem.Biophys. (first issue of each year), which are basedupon the CBN Recommendations and contain a listthereof. Reprints of these Recommendations andadvice on biochemical and chemical nomenclatureare available from the NRC Office of BiochemicalNomenclature (W. E. Cohn, Director), BiologyDivision, Oak Ridge Nat'l Lab., Box Y, Oak Ridge,Tenn. 37830 (phone: 615-483-8611, Ext. 3-7514).Normally, abbreviations (except those of standardunits of measurement and symbols of the elements)should be defined and introduced parenthetically atfirst use in the text. Enzyme activities should beexpressed in the terms set out in CBN's EnzymeNomenclature (1972) (Elsevier Scientific PublishingCo.). Lengths, weights, volumes, and molaritiesshould make use of the prefixes m, ,u, n, and p (for1o-3, 10-6, 10-9, and 10-12, respectively), whereapplicable, avoiding such compound prefixes as m,uand ,ju. Metric units should be em loyed as muchas possible. The Editors reserve the privilege ofediting manuscripts to make them conform to theabove-mentioned stylistic conventions. As a rule,all bacterial growth curves should be presented assemilogarithmic plots (log x vs. time).Form of manuscrint. All parts of the manuscript

should be typed double-space or, preferably, triple-space. Most manuscripts can and should be dividedinto the following sections: Abstract, Introduction,

Materials and Methods, Results, Discussion, Ac-knowledgments, and Literature Cited.

Title. A single statement of the subject of a paperis preferred to a main title-subtitle arrangement. Ashort version ofthe title (no more than 46 charactersand spaces) should be supplied for use as a runninghead.

Abstract. An Abstract appears at the beginning ofeach paper in the Journal. The Abstract should notexceed 200 words.

Literature Cited. In the text, references are citedby number. The Literature Cited section should betyped in alphabetical order, by first author, andnumbered. Each reference should include the title ofthe article and inclusive pagination. Names ofjournals are abbreviated according to BibliogrraphicGuide for Editors & Authors (American ChemicalSociety, 1974). Citations of abstracts, theses, "un-published data," "personal communication," andin press" will not be accepted in the LiteratureCited.

Tables. Each table should be typed on a separatepage. The data should be arranged so that columnsof like material read down, not across. Explanatoryfootnotes are permitted, but detailed descriptionsof the experiments are not. The materials andmethods used to gain the data should properlyremain in the section of that name.Figures. A complete set of figures, preferably

glossy photographs, should accompany each of thetwo copies of the manuscript. Graphs (submit asphotographs) should be finished drawings not need-ing further artwork or type-setting. Absolutely nopart of a graph should be typewritten (except thelegend, which should be typed on a separate page).Most graphs will be reduced to one-column width,and all elements in the drawing should be preparedto withstand this reduction. The legend of the figureshould provide enough information so that thefigure is understandable without reference to thetext. Experimental details from Materials andMethods should not be repeated in figure legends.Figure ordinate and abscissa scales, and tablecolumn headings, should avoid ambiguous use ofnumbers with exponents (see p. 140, CBE StyleManual).Nomenclature of microorganisms. In general,

the nomenclature presented in Bergey's Manual ofDeterminative Bacteriology (8th ed., 1974) is used.If an author challenges this nomenclature, his ownjudgment will be followed, but the name in Bergey'sManual should follow in parentheses the first timethe name is used in the text and in the Abstract.Papers which include extensive taxonomic material(e.g., description of new taxa) will not be publishedin this journal. The proper place for publication oftaxonomic material is the International Journal ofSystematic Bacteriology (IJSB), which is publishedby the ASM for the International Association ofMicrobiological Societies. If the main thrust of sucha paper is not taxonomy, the paper should be di-vided, the taxonomy portion being submitted toIJSB and the other portion to this journal.Notes. The accepted form for Notes is somewhat

different from the foregoing. Contributors shouldconsult a recent issue of the Journal for style. Notesshould not exceed 500 words. The Abstract shouldnot exceed 25 words.

Copyright. Once a paper has been published inthe Journal, which is a copyrighted publication, thelegal ownership of all parts of the paper, includingthe illustrations, has passed from the author to theASM. If the same author, or any author, wishes torepublish material previously published in theJournal, he must first receive written permissionfrom ASM.

AUTHOR INDEX

VOLUME 130

Aaronson, S., 205Abdelal, Ahmed T. H., 1125Adachi, Toshiro, 74Ahearn, Donald G., 1125Ames, Bruce N., 399, 411, 420Ananthaswamy, H. N., 187Anderson, James J., 384Aoki, Takashi, 529Arai, Toshihiko, 529Arceneaux, J. E. L., 173Arias, A., 1139Armstrong, Emma Lee, 569Arthur, Helen, 312Aswell, J. E., 173Atkinson, Daniel E., 676Atkinson, Katharine D., 472

Bagdasarian, Michael, 577Bagdasarian, Miroslawa M., 577Barbour, Stephen D., 160Barker, Susan L., 1017Bar Tana, J., 787Bassford, Philip J., Jr., 750Bayer, Manfred E., 911, 1364Bayley, Susan A.,Bean, Barry, 571Beck, Barbara D., 1292Beck, Reta S., 181Beremand, Phillip D., 532Bernard, Patricia D., 200Bishop, Paul E., 954Bjork, Glenn R., 635Blasi, F., 4Bless, Juergen W., 20Bloom, Fredric R., 983Blumberg, Richard, 852Bohlin, Thomas, 604Bolt, R. J., 545Bonner, J. T., 144Borgia, Peter, 812Botsford, J. L., 563Bowers, Blair, 1357Bowman, Barry J., 274, 285Bredt, W., 937Bremer, H., 92, 692, 1098, 1109,

1206Bridges, B. A., 724Brill, Winston J., 954Brinton, Charles C., Jr., 495,

506Broda, Paul, 1274Brodie, Arnold F., 729Brown, Byron L., 759, 948Bruni, C. B., 4Bulla, Lee A., Jr., 375Burman, Lars G., 604Burnett, L., 538Byers, B. R., 173

Calleja, G. B., 512Callister, Heather, 1030Canale-Parola, E., 485Caro, L., 303Castric, Peter A., 826Celis, T. F. R., 1234, 1244Chai, Tuu-jyi, 781Chan, Russell K., 766Chandler, M., 303Chandler, P. M., 596Chang, B. J., 943Chang, F. N., 839Charba, J. F., 242Chase, John W., 667Chen, L. J., 545Cheng, Po-Jun, 620Churchward, Gordon, 92, 692,

1206Cirillo, Vincent P., 714Cleveland, P., 1333Clewell, Don B., 759, 948Cohen, Stanley N., 888Colantuoni, V., 4Coleman, William G., Jr., 656Conner, Barbara H., 704Cooper, Stephen, 118Cooper, Terrance G., 192, 1253Cornell, C. P., 965Cortese, R., 4Costerton, J. W., 318, 329, 339Cox, B. S., 1303Cronan, John E., Jr., 114Culbertson, Michael R., 472

Danley, Dennis E., 563Davidson, Loren I., 375Davis, Rowland H., 274, 285Dawkins, C. A., 173Delisle, Gloria J., 1390Demain, A. L., 1010de Marsac, N. Tandeau, 82Deutsch, Robert, 620Dhawale, S. W., 205Dougan, G., 846Downard, J. S., 1175Drews, G., 629Drexler, Mark, 563Duggleby, Clive J., 1274Duval-Iflah, Yvonne, 1281Dworkin, Martin, 561

Eberhart, Bruce M., 181, 516Efstathiou, J. D., 257Egi, Yoshiko, 542Eisenstark, A., 187Emerson, Suzanne U., 200Endo, Akira, 48Engel, Robert, 968Erickson, Robert J., 869

i

Falkinham, Joseph O., III, 566Filpula, David, 107Finch, Lloyd R., 1047Fitzgerald, T. J., 1333Foulds, John, 781Frazier, Marsha L., 1064Frey, J., 303Friebel, T. E., 1010Friedman, Stanley A., 991Fuchs, James A., 107, 957, 960Fukuda, Akio, 1199Fridovich, Irwin, 805

Gardiol, A., 1139Gelfand, David H., 429, 441Gibson, D. T., 1117Giles, Norman H., 704, 1192Glaser, Luis, 610Glazer, Alexander N., 200Goebel, Paul J., 656Goldberg, I., 535Gonzalez, Iris Laudien, 684Goolsby, Kathy M., 181Gotfried, Fern, 963Gotschlich, Emil C., 775Graham, Amy C., 1214Green, M. H. L., 724Greenberg, E. P., 485Grindley, June N., 852Gronostajski, Richard M., 793Grosch, Josephine C., 869Gross, Tracy A., 114Guest, John R., 1038Gunja-Smith, Zeenat, 818

Hacking, Andrew J., 832Hall, Ruth M., 973Harada, Tokuya, 62, 74Harmon, Jeffrey M., 1224Hassan, H. Moustafa, 805Hatfield, G. Wesley, 552Hautala, Judith A., 704Haydon, A. H., 173Hayflick, L., 292, 297, 464Hays, John B., 991Henry, Susan A., 472Henson, Joan M., 354Hershberger, Charles L., 860Hildebrandt, Virginia A., 37Hill, Aubrey, 558Hilmen, M., 877Hodson, Robert C., 266Hohman, R. J., 1345Holloway, B. W., 943Holm, C., 1303Holmlund, C. E., 1310Howlett, Barbara J., 787

ii AUTHOR INDEX

Imperato, T. J., 545Indira, R., 629Ippen-Ihler, Karin, 495, 506Irvin, R. T., 318, 329, 339Israel, Daniel W., 793Israeli, Eitan, 729Izakowska, Maryla, 577

Jacobson, Eric S., 1397Jacobson, James W., 704, 1192James, Eric, 642Janssen, Gary R., 561Jensen, A. P., 535Johnson, Byron F., 512Johnson, R. C., 1333Jones, Gary E., 128Jones, Jay B., 1357, 1404Juretic, Davor, 524

Kadner, Robert J., 750Kakihara, Katsushi, 62Kalra, Vijay K., 729Kao, M., 965Kashket, Eva R., 1017Kawasaki, Takashi, 542Keating, Mark, T., 144Kennedy, Emily H., 1125Kennedy, Eugene P., 1072Kier, Larry D., 399, 411, 420Kiesow, Lutz A., 20Kim, Sangduk, 839Kline, Ellis L., 951Kobayashi, G. S., 1387Kodaira, Ryoji, 212Kondoh, Hisato, 736Kolat, Anita I., 472Kolenbrander, P. E., 1345Konisky, J., 1399Konrad, E. Bruce, 167Koshland, D. E., Jr., 223, 787Kosman, Daniel J., 455Kramer, Karl J., 375Kretschmer, Peter J., 888Krishnapillai, V., 596Kumar, B. Vijay, 1387Kushner, D. J., 698Kwiatowski, Bartlomiej, 366

Lam, Keng-Bon, 746Landau, J. V., 154Langworthy, Thomas A., 1326Lawther, Robert P., 552Lee, Chong S., 354Lee, Theresa, 37LeGall, Jean, 1084Leifer, Zev, 968Leive, Loretta, 656, 1364Le Mesurier, Sue, 1030Lev, Meir, 445Lew, Betty, 839Lin, E. C. C., 832Lindsay, Barbara, 610Lindsley, Benjamin F., 20Liu, Ying-ping, 495, 506Ljungdahl, Lars G., 1084

Long, Richard A., 1159Lucas, M. Cran, 1192

Maas, Werner K., 1402MacAlister, T. J., 318, 329, 339McCready, S. J., 1303McKay, L. L., 257McKibbin, Janet, 1000McLaughlin, C. S., 1303McMillian, Ray, 1387Manning, Paul A., 540Manross, Delbert N., Jr., 951Manwaring, John D., 960Martin, W. G., 1159Marmur, Julius, 746Martinez-Drets, G., 1139Masker, Warren E., 667Masover, G. K., 292, 297, 464Matsumura, P., 877Mattick, John S., 973Mayer, H., 629Medoff, Gerald, 1387Messenguy, Francine, 1253Metzenberg, Robert L., 1397Michelsen, Ole, 136Mielenz, Jonathan R., 860Mikheyskaya, L. V., 1Milford, Albert F., 445Miller, Charles G., 347Miller, J. N., 1333Miller, Judith E., 160Milner, Joel J., 860Mitchell, Alana, 1047Miwa, Toshiaki, 62Miwatani, Toshio, 1393Mottershead, R. P., 724Muller, Eric, 114Munford, Robert S., 775Murooka, Yoshikatsu, 62, 74

Nakada, Dai, 852Nakata, H. M., 242Natter, William, 642Neidhardt, Frederick C., 212Niemann, Heiner, 366Norwood, William I., 100Nunn, William D., 620Ny, Tor, 635

O'Brien, R. W., 131Ohnishi, Takeo, 1393Okada, Yoshimi, 1199Okamura, Haruki, 74Oliver, Daphna R., 759, 948Oliver, S. G., 1303Ovodov, Yu. S., 1Ovodova, R. G., 1Oxender, Dale L., 384, 1024

Pace, Norman R., 1000Palant, Marina, 464Palchaudhuri, Sunil, 1402Park, James T., 1292Patel, Gordhan L., 704Patil, Narayan B., 818

Patni, N. J., 205Paznokas, John L., 661Pearce, Susanne M., 37Pope, D. H., 154Pugsley, Anthony P., 26

Quay, Steven C., 1024

Radestock, U., 937Rahn, Shelley, 1402Raibaud, Pierre, 1281Razin, S., 292, 297, 464Reddy, C. Adinarayana, 965Reeves, Peter, 26, 540Rogers, J. E., 1117Rogers, P. J., 521Rolfe, Barry G., 939Rousseau, Micheline, 1281Rownd, Robert H., 148, 1262Rudo, Naomi, 441Russo, V. E. A., 548

Sadler, John R., 100Sanemori, Hiroshi, 542Santer, Melvin, 900Santos, Diogenes S., 1402Sbordone, L., 4Schamhart, D. H. J., 526Schenk, S. P., 1382Schlessinger, David, 1387Schmidt, Robert R., 793Schnaitman, Carl A., 750Schneider, Henry, 1159Schroeder, Ellen, 1000Scott, William A., 1144Selitrennikoff, Claude P., 249Sens, Don, 642Setlow, Barbara, 1130Setlow, Peter, 1130Seto, Koji, 62Shane, Sara, 900Shaper, Joel H., 200Shatzman, Allan R., 455Shen, V., 1098, 1109Sheppard, David E., 684Sherratt, I. J., 846Shindler, D. B., 698Silver, L., 303Silverman, M., 877, 1317Simon, Melvin I., 200, 877, 1317Singh, Ravendra P., 1130Skotnicki, Mary L., 939Smith, Eric E., 818Smith, Paul F., 393Smith, W. P., 154Snider, Martin D., 1072Sobus, M. T., 1310Sogin, Mitchell L., 1000Sojka, Gary A., 532Sonneborn, David R., 249Soucek, S., 1399Spencer, Allen, 114Sprague, George F., Jr., 232Stadtman, Thressa C., 1357,

1404

J. BACTERIOL.

VOL. 130, 1977

Steinberg, Robert A., 429Stirm, Stephan, 366Stocker, B. A. D., 1214Streicher, Stanley L., 983Sutherland, P. A., 1303Swaney, Lois M., 495, 506Sykes, J. A., 1333Sypherd, Paul S., 661, 812

Taber, Harry W., 1224Taichman, Lorne, 1262Takayama, Kuni, 569Takeda, Yoshifumi, 1393Tanaka, Yoshinori, 1393Tang, Chu T., 620Taylor, Barry L., 131, 223Taylor, Dean P., 148Thomas, Terence D., 583Thompson, John, 583Thurow, Horst, 911To, Cheng-chin, 495To, Chuen-mo, 495Tockman, Judith, 1091Tomasz, Alexander, 571Tropp, Burton E., 620, 968

AUTHOR INDEX

Tsujita, Yoshio, 48Turner, H. R., 173Tyler, Bonnie, 192, 983

Unger, Michael W., 11

van den Heijkant, M. P. M., 526van de Poll, K. W. 526van der Plaat, Johannes B., 946Van Heijenoort, Jean, 1281van Solingen, Pieter, 946Villadsen, Ingrid S., 136Vold, Barbara S., 1091

Wake, R. G., 538, 1030Walker, James, R., 354Walker-Simmons, Mary, 676Wang, Augustine W., 558Wanner, Barry L., 212Ward, J. Barrie, 1055Warrick, H. M., 223Warwick, Mary L., 951Watson, Kenneth, 312Wechsler, James A., 963Weckesser, J., 629

Weinberger, Martin, 118Weppelman, Roger, 399, 411, 420Westphal, Ulrich, 366Whittaker, N. F., 1310Williams, Peter A., 1149, 1274Williams, Stuart K., II, 266Wireman, John W., 561Womble, David D., 148Wong, C. G., 545Woodward, John R., 714Woolfolk, C. A., 1175Worsey, Michael J., 1149, 1274Wydro, R. M., 698Wyke, Anne W., 1055Wyss, Orville, 1382

Yang, Shiow-Shong, 1084Yeung, Anthony T., 793Yoh, Myonsun, 1393Yoo, Bong Y., 512Young, R., 92Yudkin, M. D., 57

Zimmerman, Leonard N., 1064Zipser, David, 347

* *

SUBJECT INDEX

VOLUME 130

O-Acetylhomoserine sulfhydrylasecatalyzing O-alkylhomoserine, 62

Acholeplasmalipopolysaccharides, homogeneity, 393

Adenylate energy chargein E. coli under nutritional stress, 676

a FactorS. cerevisiae a cells, 766

Alkaline phosphatasecell envelope protection against acid denaturation

in E. coli, 339of B. emersonii, 249

Alkaline phosphatase, cell surface-localizedofE. coli, 318

O-Alkylhomoserine synthesisC. acetophilum, 62catalyzed by O-acetylhomoserine sulfhydrylase,

62Alteromonas halolanktis

neutral amino acid transport, 37Amino acid transportbinding sites in M. phlei, 729E. coli, 1024transcription termination factor Rho in E. coli,

1024Aminoacylation

of tRNA in B. subtilis, 1091Anabaena flos-aquae

phenol-water-extractable materials, 558an-Glycerol 3-phosphate acylation

phenethyl alcohol inhibition in E. coli, 620L-Arabinose operon

ofE. coli, mutations in, 684argA, argE, and argCBH operons

of E. coli, transcription, 642Arginine catabolism

cycling of ornithine, 285in Neurospora, 285

Arginine in Escherichia coli K-12regulation of transport and biosynthesis, 1244

Arginine transportE. coli K-12 mutant defective in, 1234

Aromatic amino acid aminotransferasedeficiency in E. coli mutants, 429mapping of genes tyrB and aspC, 441

Arthrobacter crystallopoietescell division of, 1345electron microscopic study of cell surface rings,

1345morphogenesis of, 1345

Arylsulfatasecontrol in a serine auxotroph ofNeurospora, 1397

Arylsulfatase synthesisgenetic control, 74K. aerogenes, 74

L-Asparaginase Igenetic and physiological relationships with as-

paraginase II, 128

S. cerevisiae, 128Asparaginase II

genetic and physiological relationships with I-as-paraginase I, 128

S. cerevisiae, 128Aspartate aminotransferaseE. coli mutants deficient in, 429mapping of genes tyrB and aspC, 441

aspCmapping of aminotransferase, 441

Aspergillus oryzaeassociation with heterogeneous polysaccharides,

48extracellular acid protease, 48

Azotobacter vinelandiigenetic analysis of mutant strains, 954influence of oxygen in phospholipid productionand colony formation, 1382

mutant strains unable to fix nitrogen, 954nitrogen-fixing mutant, 1382

Bacillus amyloliquefacienspolynucleotide phosphorylase, 869

Bacillus brevisgramicidin S synthetase, 1010oxygen-dependent inactivation of gramicidin S

synthetase, 1010Bacillus cereus

sporogenesis, role of glutamate, 242Bacillus megaterium

ferriferrioxamine B in membrane vesicles, 173ferrischizokinen in membrane vesicles, 173levels of small molecules and enzymes, 1130siderophore receptors in membrane vesicles, 173

Bacillus subtilisaminoacylation of tRNA, 1091biosynthesis of wall polymers, 1055cell wall turnover and cell growth, 610classification of flagellins, 200dna initiation mutant, altered accumulation of amembrane protein, 1244

precursor-specific RNA fragments, 1000Bacillus subtilis 168dnaB(Ts)134 mutation, 1030DNA replication in germinating spores carryingdnaB(Ts)134 mutation, 1030

Bacillus subtilis TsB134chromosome replication in the DNA initiationmutant, 538

Bacillus thuringiensisentomocidal parasporal crystal, 375

Bacterial lysatescovalently closed circular plasmid-to chromosome

ratios, 148dye-buoyant density centrifugation, 148

Bacteriocin productiondetermined by pOBl and pOB2, in S. faecalis, 948

Bacteriophage-borne glycanaseiv

VOL. 130, 1977

depolymerizing Klebsiella serotype 25 capsularpolysaccharide, 366

Bacteriophage-resistant mutantsrecipient ability in E. coli K-12, 540

Bacteriophages T5, Ti, and ES18sensitivity of Salmonella sp., 1214

Bacteroides melaninogenicussphingolipid precursors and fatty acids, energy-dependent incorporation, 445

Barotolerant protein synthesisinitiation factors, 154P. bathycetes, 154specific ion concentration, 15430S ribosomal subunit, 154

bfe gene productsfunctional stability in E. coli, 750

Blastocladiella emersonjialkaline phosphatase, 249

Blue-green algaeisolation and characterization of lipopolysaccha-

rides from cell walls, 1lipopolysaccharides, 1Phormidium, 1

Branched-chain amino acidsalternately required byE. coli K-12 mutant, 566

Branched-chain amino acid transport systemslack of binding protein and other components inE. coli transport mutants, 384

BUG 6carboxypeptidase II activity during the cell cycle,

1292temperature-sensitive division mutant of E. coli

K-12, 1292

Candida lypolyticacell surface topography, 312

Candida parapsilosisoxidative phosphorylation in continuous cultures,

521Candida slooffi

cell surface topography by scanning electronmicroscopy, 312

Carboxypeptidase II activitycoordinate morphogenesis of polar surface struc-

tures, effect of macromolecular synthesis, 1199in temperature-sensitive mutant of E. coli K-12,

1292observed fluctuation in BUG 6, 1292

Caulobacter crescentusCell-bound cations

of V. costicola, 698Cell surface rings of A. crystallopoietes

electron microscopy during cell division and mor-phology, 1345

Cellular slime moldsnegative chemotaxis, 144

CellulaseofN. crassa, 181

Chemotactic mechanismS. typhimurium, 223

Chemotaxiscellular slime molds, 144identification of polypeptides in E. coli, 1317

Chemotaxis genescarried by Lambda E. coli hybrids, 877

Chlamydomonas reinhardi

SUBJECT INDEX v

extracellular phosphatases, 205urea transport at low concentrations, 266

Chloramine Tiodination of E. coli, 775

Chlorellacontrol of GDH induction, 793

Chloroplast deoxyribonucleic acidof E. gracilis, analysis, 860

Choline metabolismin pneumococci, 571

Chromatic adaptationcyanobacteria, 82occurrence and nature, 82

Chromosome replicationin TsB134 of B. subtilis, 538

cis-Toluene dihydrodiol dehydrogenaseP. putida, 1117

Clostridium thermoaceticumhigh thermostability in a four-iron, four-sulfide

ferredoxin, 1084Colicin I

outer membrane receptor protein of E. coli, 1399Colicin I outer membrane receptor protein

iron-enterochelin uptake, 1399Colicin M

sensitivity of Salmonella sp., 1214Colony formation

in a nitrogen-fixing mutant ofA. vinelandii, 1382influence of oxygen, 1382

Conjugation-induced lysisof S. pombe, 512

Coordinate morphogenesisaffected by macromolecular synthesis, 1199of polar surface structures, in C. crescentus, 1199

Corynebacterium acetophilumO-alkylhomoserine synthesis, 62

Corynebacterium pyogeneshemin-dependent growth stimulation and cyto-chrome synthesis, 965

Covalently closed circular plasmid-to-chromosomeratios

bacterial lysates, 148dye-buoyant density centrifugation, 148

Cyanobacteriachromatic adaptation, 82phycoerythrin producing, 82

Cyclic adenosine 3',5'-monophosphatedifferential binding to cAMP receptor protein inE. coli, 563

Cyclic phosphodiesteraseof S. typhimurium, 411regulation in S. typhimurium, 420

Cytochrome synthesishemin-dependency in C. pyogenes, 965

Dactylium dendroidesgalactose oxidase synthesis and secretion, 455pH and culture density in galactose oxidase syn-

thesis and secretion, 455Decontrolled lac operon

physiological regulation, 212Defective nitrogen-fixing phenotypes

selection inE. coli K-12 by dimethyl sulfoxide, 939Degradation of precursor-specific ribonucleic acid

fragmentsB. subtilis, 1000

vi SUBJECT INDEX

Deoxyribonucleic acidfreeze-drying in E. coli, 1393induction and repair of single-strand breaks in E.

coli, 187repair of strand breaks during freeze-drying, 1393R-plasmid-mediated restriction-modification sys-tem conferred by group E R plasmids, 529

strand breaks during freeze-drying, 1393Deoxyribonucleic acid damage

cell division in E. coli B,.12, hypersensitivity byultraviolet light, 724

Deoxyribonucleic acid initiation mutantofB. subtilis, chromosome replication at 45°C, 538

Deoxyribonucleic acid repairin E. coli mutants, 667

Deoxyribonucleic acid replicationdnaB(Ts)134 mutation ofB. subtilis, 1030in bacteria, 92in germinating spores of B. subtilis, 168, 1030initiation and termination, 92stepwise increase in velocity of replication, 92

Deoxyribonucleic acid replication timein exponentially growing E. coli B/r, 1206

Deoxyribonucleic acid segregationE. coli, 118

Deoxyribonucleic acid segregationmedium-dependent variation, 118

Deoxyribonucleic acid synthesisinhibition of replication initiation inE. coli, 692isolated mitochondria from S. cerevisiae, 973

Dictyostelium discoideumnegative chemotaxis, 144

3,4-Dihydroxybutyl-1-phosphonateanalogue of sn-glycerol 3-phosphate, 968transport, 968

Dimethyl sulfoxideaffecting selection of defective nitrogen-fixingphenotypes, 939

dnaA+dominance to dnaA in E. coli, 963

dnaA508 mutationin E. coli, dominance of dnaA+, 963

dnaA mutation of E. coliR factor R100.1 generation of small plasmids after

integration, 303suppression by integrated R factor R100.1, 303

dnaA phenotypesuppression by mutations in the rpoB cistron ofRNA polymerase, 577

dnaB(Ts)134 mutationB. subtilis, 1030DNA replication, 1030

dna initiation mutantof B. subtilis, altered accumulation of a mem-brane protein, 1224

XdnaZ+ transducing phagesofE. coli, 354

Dye-buoyant density centrifugationcovalently closed circular plasmid-to-chromosome

ratios, 148

EcoRIE. gracilis chloroplast DNA analysis, 860

Effects of potassium ionselectrical and pH gradients across membrane ofS.

lactis cells, 1017

Electron microscopyofA. crystallopoietes during cell division and mor-

phology, 1345Electron transport mutants

in E. coli, flagellar formation, 787Entomocidal parasporal crystal

ofB. thuringiensis, 375Enzymatic methyl esterification

ofE. coli ribosomal proteins, 839Escherichia coli

adenylate energy charge under nutritional stress,676

amino acid transport, 1024argA, argE, and argCBH operons, in vitro tran-

scription, 642bfe and tonB gene products, 750biochemical characterization of an hisT strain,

552biochemical properties, 4catabolic pathway for propanediol, 832cell envelope protection of alkaline phosphatase

against acid denaturation, 339cell surface-localized alkaline phosphatase, 318chemotaxis, 1317chromosomal duplications, 167colicin I, lack of in mutants, 1399degradation of /8-galactosidase fragments in S.typhimurium, 347

differential binding of cAMP by cAMP receptorprotein, 563

DNA replication, 92DNA segregation, 118DNA strand breaks and repair during freeze-

drying, 1393DNA synthesis after inhibition of replication ini-

tiation, 692dominance of dnaA + to dnaA, 963effect of EDTA on surface, 1364energy requirements for methylthio-fB-i-galacto-

side, 1159enzymatic methyl esterification, 839ferrienterochelin uptake, 26functional capacities under nutritional stress, 676glycerophosphate acyltransferase, 1072hyper-rec mutants, 167immunocytological investigation of protein syn-

thesis, 329iodination with chloramine T, 775iron-enterochelin uptake, 1399flagellar formation in electron transport mutants,

787medium-dependent variation, 118motility influenced by naturally occurring conju-

gative plasmid, 604nucleoside tri- and tetraphosphate pools, 136outer membrane lipoprotein, selective labeling,

775phenethyl alcohol inhibition ofan-glycerol 3-phos-phate acylation, 620

physiological regulation in a decontrolled lac op-eron, 212

plasmids in a sucrose-fermenting strain, 1402polypeptides, 1317polysaccharide capsule, microscopy of, 911PRPP pools, 136recombination-deficient strains, 160

J. BACTERIOL.

VOL. 130, 1977

regulatory properties, 4repression of tryptophanase synthesis, 57ribonucleoside diphosphate reductase synthesis,

10730S and 5S ribosomes, 900single-strand breaks, repair of, 187stepwise increase in velocity of replication, 92superoxide dismutase in glucose-limited chemo-

stat cultures, 805suppression of a dnaA mutation by the integratedR factor R100.1, 303

suppression ofdnaA phenotype by rpoB mutationsof RNA polymerase, 577

synthesis of ribonucleoside diphosphate reductasecomponents, 957

transcription termination factor Rho upon regula-tion of amino acid transport, 1024

transporter of methylthio-,8-n-galactoside, 1159tRNA (m5U)methyltransferase from, 635type 1 pilus mutants and phase variants, 495unstable mutations, 57viable, residually dividing and nondividing cell

classes, 160Escherichia coli 0111:B4

genetic analysis, 656Escherichia coli B/rDNA replication time, 1206mutations in the L-arabinose operon, 684reduced initiator function in mutations of L-arabi-nose operon, 684

RNA elongation during chloramphenicol treat-ment, 1109

rRNA, tRNA, and mRNA, synthesis of, 1098Escherichia coli B.,2

cell division, 724hypersensitivity to DNA damage, 724

Escherichia coli dnaA mutationgeneration of small plasmids after integration byR factor R100.1, 303

Escherichia coli K-12bacteriophage-resistant mutants, recipient abil-

ity, 540BUG 6, 1292carboxypeptidase II activity, 1292defective nitrogen-fixing phenotypes, 939hisT mutants, 4ilvA deletion strain, 951isoleucine-valine transaminase in an ilvA dele-

tion strain, 951menaquinone biosynthesis, 1038mutants requiring 2-succinyl-benzoate, 1038temperature-sensitive division mutant, 1292tolF mutants, 781transport and biosynthesis of arginine, 1244

Escherichia coli K-12 mutantalternately requiring branched chain amino

acids, 566alternately requiring vitamin B6, 566arginine and ornithine, transport defects in, 1234lacking transaminase C activity, 566

Escherichia coli minicellsnucleotide triphosphate pools, 960

Escherichia coli mutantsdeficiency in 5'-.3' exonuclease activity of DNApolymerase I and exonuclease VII, 667

SUBJECT INDEX vii

deficient in aminotransferases, 429DNA repair, 667isolation, 167

Escherichia coli transport mutantslacking components of branched-chain amino acid

transport systems, 384Escherichia coli type 1

somatic pilus mutants, genetic complementationanalysis, 506

Ethylenediaminetetraacetateeffect upon E. coli surface, 1364

Ethylene-induced growthin Phycomyces mutants abnormal for autochemo-

tropism, 548Euglena gracilis

chloroplast DNA analysis with EcoRI, 860Exonuclease VII

of DNA, deficiency in E. coli mutants, 6675'--+3' Exonuclease activity of DNA polymerase I

and exonuclease VIIdeficiency in DNA repair in E. coli mutants, 667

Extracellular acid proteaseA. oryzae, 48association with heterogeneous polysaccharides,48

multiple forms, 48Extracellular phosphatases

of C. reinhardi, 205

Fatty acid compositionof methanol-utilizing bacteria, 535

Fatty acid metabolism of S. cerevisiaeeffects of trifluperidol, 1310

Fatty acidsenergy-dependent incorporation in B. melanino-genicus, 445

Ferredoxinfrom C. thermoaceticum, 1084with high thermostability, 1084

Ferrienterochelinenergy-dependent stage of uptake, 26uptake in E. coli, 26

Ferriferrioxamineof B. megaterium, 173

Ferrischizokinenof B. megaterium, 173

Ferritin-labeled antibodiesvisualization ofE. coli alkaline phosphatase, 318

Flagellinsclassification in B. subtilis, 200

fla genesof E. coli K-12, carried by nondefective transduc-

ing lambda bacteriophage, 736F' plasmid deoxyribonucleic acid

differential association with a P. mirabilis lysate,1262

Free mycolic acidsin M. tuberculosis, 569

Fructose diphosphataseinactivation by sucrose in yeast, 526

Fucose systemcatabolic pathway for propanediol inE. coli, 832

Galactose oxidase synthesis and secretionpH and culture density in D. dendroides, 455

viii SUBJECT INDEX

,8-Galactosidase fragments of Escherichia colidegradation in protease-deficient mutants of S.typhimurium, 347

Gl arrestrecovery of S. cerevisiae mating type a cells from,by a factor, 766

S. cerevisiae, 11Germ tube formation

inhibition in N. crassa, 516,S-Glucosidase synthesis

control in M. racemosus, 812Glutamate

in sporogenesis of B. cereus, 242Glutamine synthetaseenzyme synthesis, 983S. typhimurium, 983

Gramicidin S synthetaseB. brevis, 1010oxygen-dependent inactivation in B. brevis, 1010

Group E R plasmidsR plasmid-mediated restriction-modification sys-tem of DNA, 529

sn-Glycerol 3-phosphatetransporting 3,4-dihydroxybutyl-1-phosphonate,

968Glycerophosphate acyltransferase

partial purification from E. coli, 1072Glycogen

in Saccharomyces, 818Glycolytic pathway mutants

isolation and characterization in S. cerevisiae, 746

Halophilic bacteriumV. costicola, cell-bound cations of, 698

Hemin-dependent growth stimulationin C. pyogenes, 965

Hemolysin productiondetermined by pOB1 and pOB2, in S. faecalis, 948genetic loci in S. faecalis subsp. zymogenes, 1064

Heterogeneous polysaccharidesof acid protease, 48

Hexitol phosphoenol pyruvate-dependent phospho-transferases

S. aureus, 991Hexose phosphoenol pyruvate-dependent phospho-

transferasesS. aureus, 991

Histoplasma capsulatum ribonucleic acid polymer-ases

inhibition by homologous and heterologous RNA,1387

hisT strainof E. coli, biochemical characterization, 552

Homologous and heterologous RNAinhibiting H. capsulatum RNA polymerases, 1387

hut operonsof S. typhimurium, 192transcription, 192

Hydrogen cyanide biosynthesisby P. aeruginosa, 826

Hyper-rec mutantsenhanced recombination in E. coli, 167

ilvA deletion strainof E. coli, multivalent regulation of isoleucine-

valine transaminase, 951

J. BACTERIOL.

Inactivation of fructose diphosphataseby sucrose in S. carlsbergenesis, 526

Independent regulation of transport and biosyn-thesis

of arginine in E. coli K-12, 1244Inducible glutamate dehydrogenaseaccumulation and turnover in Chlorella, 793

Inhibition of deoxyribonucleic acid synthesisribonucleoside diphosphate reductase synthesis,

107Inorganic salts resistance

in S. lactis, 257with a lactose-fermenting plasmid, 257

Inositol-starved Saccharomyces cerevisiaegrowth and metabolism, 472

Integrated R factor R100.1suppressing E. coli dnaA mutation, 303

Iron-enterochelin uptakeE. coli mutants, 1399mutants ofE. coli lacking the colicin I outer mem-brane receptor protein, 1399

Isolated mitochondriareplicative DNA synthesis, 973S. cerevisiae, 973

Isoleucine-valine transaminasemultivalent regulation in E. coli K-12 ilvA, 951

K-12 hisT mutantsbiochemical properties, 4E. coli, 4regulatory properties, 4

Klebsiella aerogenesgenetic control of arylsulfatase synthesis, 74

Klebsiella serotype 25 capsular polysaccharidedepolymerization by a bacteriophage-borne gly-

canase, 366primary structure, 366

lac operonin E. coli, 212

3-Lactamasein vitro system, 852R-plasmid-coded, 852synthesis in minicells, 852

Lactose-fermenting plasmid in Streptococcus lactisinorganic salts resistance, 257

Lactose operator-constitutive mutantspseudoreversion, 100

Lambda Escherichia coli hybridscarrying chemotaxis genes, 877

Lecithinrequirement for sporulation in N. crassa, 524

Leucosporidium frigidumcell surface topography by scanning electron mi-

croscopy, 312Leucosporidium nivalis

cell surface topography, 312Lipid A

fatty acyl moieties, 114relA gene control of the synthesis, 114

Lipophilic 0-antigensR. tenue, 629

Lipopolysaccharideshomogeneity of, from Acholeplasma, 393Phormidium, 1

Lipopolysaccharides of blue-green algae

VOL. 130, 1977

isolation and characterization, 1Lithocholate sulfate

hydrolysis by P. aeruginosa, 545Lipid composition

of heterotrophically and autotrophically grown S.acidocaldarius, 1326

Lysine-isoaccepting transfer ribonucleic acid speciesdifferential utilization, 1091

Lysogenic conversionS. aureus, 1281

Macromolecular synthesiseffect on coordinate morphogenesis of polar sur-

face structures, 1199in C. crescentus, 1199

Marine pseudomonadneutral amino acid transport, 37

Mating-type a cellsrecovery from Gl arrest by a factor, 766

Membrane protein, altered accumulationin a dna initiation mutant of B. subtilis, 1224

Menaquinone biosynthesisE. coli K-12 mutants, 1038mutants of E. coli K-12 requiring 2-succinylben-

zoate, 1038Messenger ribonucleic acidchloramphenicol-induced changes in synthesis,

1098synthesis in E. coli B/r, 1098

Methanococcus vannieliiculture, 1404growth, effects of selenium and tungsten, 1404sensitivity to detergents and antibiotics, 1357ultrastructure, 1357

Methanol-utilizing bacteriaphospholipid and fatty-acid composition, 535

Methionyl-transfer ribonucleic acid deficiencyGl arrest of S. cerevisiae, 11

Methylthio-,8-n-galactosideenergy requirements by E. coli, 1159measurement by microcalorimetry, oxygen con-sumption, and carbon dioxide production, 1159

transport by E. coli, 1159Microcalorimetrymeasuring transport of methylthio-38-D-galacto-

side, 1159Micrococcus denitrificans

specific thiamine monophosphate phosphohydro-lase, 542

Mitomycin C inductionof wild-type and mutant CloDF13 plasmids, 846

Mucor racemosuscontrol of f8-glucosidase synthesis, 812pyruvate kinase isozyme regulation, 661

Mutant CloDF13 plasmidsmitomycin C, 846proteins synthesized from, 846

Mycobacterium phleibinding sites for amino acid transport, 729

Mycobacterium tuberculosismetabolism in free mycolic acids, 569

Mycoplasma mycoides subsp. mycoidesnucleotide biosynthesis, 1047

Mycoplasma pulmonisgliding motility, 937

SUBJECT INDEX ix

Myxococcus xanthusgrowth affected by temperature, 561

Naturally occurring conjugative plasmidpUM5, influencing motility ofE. coli and S. typhi-murium, 604

Neurosporaanabolic and catabolic steady states of ornithine,

274arginine catabolism, cycling of ornithine, 285control of arylsulfatase in a serine auxotroph,

1397serine auxotroph, 1397

Neurospora crassacellulase, 181characterization ofpolyadenylated mRNA, 1192germ tube formation, inhibition of, 516in vitro translation of polyadenylated mRNA,

1192lecithin requirement for sporulation, 524nuclei, isolation and characterization, 704polyadenylated mRNA, 1192unsaturated fatty acid mutants, 1144

Neutral amino acid transportmarine pseudomonad, 37

Neutral proteaseS. lipolytica, 1125

Nitrobacter winogradskyiinhibition by pyridoxal phosphate, 20phosphoribulokinase, 20

Nitrogen-fixing mutantof A. vinelandii, phospholipid production and

colony formation, 1382Nitrogen-starved yeastamino acid transport and metabolism in S. cerevi-

siae, 714Nondefective transducing lambda bacteriophages

carrying fla genes of E. coli K-12, 736isolation and characterization, 736

Nucleoside tri- and tetraphosphate poolsE. coli, 136nitrogen starvation, 136

Nucleoside triphosphate poolsin E. coli minicells, 960

Nucleotide biosynthesisMycoplasma mycoides subsp. mycoides, 1047

0-antigens (lipopolysaccharides)of R. tenue, 629

Ornithinecellular distribution in Neurospora, 274

Ornithine carbamoyltransferase productionoccurring at the transcription level of S. cerevi-

siae, 1253specific and "general" control in S. cerevisiae, 1253

Ornithine cyclingarginine catabolism in Neurospora, 285

Ornithine in Neurosporaanabolic and catabolic steady states, 274

Ornithine transportE. coli K-12 mutant defective in, 1234

Oxalacetateformation and dissimilation, 131grown on noncarbohydrate substrates, 131P. citronellis, 131

x SUBJECT INDEX

Oxidative phosphorylationof C. parapsilosis, continuous cultures, 521

Periplasmic phosphatasesof S. typhimurium, 399

Phenethyl alcohol inhibitionin E. coli, 620of an-glycerol 3-phosphate acylation, 620

Phenol-water extractionofA. flos-aquae, 558

Phormidiumlipopolysaccharides, 1

Phosphatasesregulation in S. typhimurium, 420

6-Phospho-D-gluconate:NAD+ 2-oxido-reductase (de-carboxylating)

from slow-growing rhizobia, 1139Phosphoenolpyruvateendogenous energy source in S. lactis, 583

2-Phosphoglycerateendogenous energy source in S. lactis, 583

Phospholipid compositionof methanol-utilizing bacteria, 535

Phospholipid productionin a nitrogen-fixing mutant ofA. vinelandii, 1382influence of oxygen, 1382

Phosphoribulokinaseactivation by NADH, 20inhibition by pyridoxal phosphate, 20N. winogradskyi, 20

Phosphotransferases of Staphylococcus aureushexose and hexitol phosphoenol-pyruvate depend-

ency, 991Phycomyces mutants abnormal for autochemotrop-

ismethylene-induced growth, 548

pil mutantscomplementation, E. coli, 506

Plaque-forming XdnaZ+ transducing phagesisolation and characterization, 354

Plasmid deoxyribonucleic acidcariogenic strain of S. faecalis, 759identification of genetic determinants on cryptic

plasmids, 759Plasmid genes

selected translocation, 888Plasmid maintenance

affected by risA in P. aeruginosa, 943Plasmids

characterization in E. coli, 1402sucrose-fermenting strain ofE. coli, 1402

Plasmids determining hemolysin and bacteriocinproduction

in S. faecalis 5952, 948Pneumococci

choline metabolism, 571Polyadenylated messenger ribonucleic acid

characterization and in vitro translation, 1192from N. crassa, 1192

Polymerase Iof DNA, deficiency in E. coli mutants, 667

Polynucleotide phosphorylasefrom B. amyloliquefaciens, 869

Polypeptidesnecessary for chemotaxis in E. coli, 1317

Polysaccharide capsuleofE. coli, microscopy, 911

Polysphondylium violaceumnegative chemotaxis, 144

Precursor-specific ribonucleic acid fragmentsmode of degradation, 1000

Propanediolevolution of a catabolic pathway in E. coli, 832

Protein synthesisimmunocytological investigation in E. coli, 329

Proteus mirabilisrapidly sedimenting lysate, differential associa-

tion of F' and R plasmid DNA with, 1262PRPP poolsE. coli, 136nitrogen starvation, 136

Pseudomonad B-16neutral amino acid transport, 37

PseudomonasTOL plasmids, 1274

Pseudomonas aeruginosaderepressed R-plasmids, 596effecting glycine metabolism, 826hydrogen cyanide biosynthesis, 826hydrolysis of lithocholate sulfate, 545risA affecting plasmid maintenance, 943

Pseudomonas bathycetesbarotolerant protein synthesis, 154

Pseudomonas citronellolisoxalacetate and pyruvate, 131

Pseudomonas putidacis-toluene dihydrodiol dehydrogenase, 1117

Pseudomonas putida MT20regulatory implications of a spontaneously occur-

ring mutant of the T0120 plasmid, 1149T0120 plasmid, spontaneous occurrence, 1149

Pseudoreversion of mutantslacOc, 100

pUM5influencing motility of E. coli and S. typhimu-rium, 604

Purification of glycerophosphate acyltransferaseE. coli, 1072

pykl of S. cerevisiaedeficient in pyruvate kinase activity, 232

Pyridoxal phosphateinhibition, 20phosphoribulokinase from N. winogradskyi, 20

Pyruvateformation and dissimilation, 131grown on noncarbohydrate substrates, 131P. citronellolis, 131

Pyruvate kinase activitydeficiency in an S. cerevisiae mutant, 232

Pyruvate kinase isozymescontrol of synthesis by glucose, 661of M. racemosus, 661

Reaction product depositionvisualization of E. coli alkaline phosphatase, 318

Recombinationisolation of E. coli mutants, 167

rec- strains of E. colirecombination deficiencies, 160

Reduced nicotinamide adenine dinucleotide

J. BACTERIOL.

VOL. 130, 1977

activation, 20phosphoribulokinase, 20N. winogradskyi, 20

relA gene controlsynthesis of lipid A fatty acyl moieties, 114

Relation between cell wall turnover and cell growthin B. subtilis, 610

Replication in Escherichia coliDNA synthesis after inhibition of initiation, 692

Replicative deoxyribonucleic acid synthesisin isolated mitochondria, 973S. cerevisiae, 973

Rhizobia6-phospho-D-gluconate:NAD+ 2-oxidoreductase

(decarboxylating), 1139slow-growing, 1139

Rhoregulation of amino acid transport in E. coli, 1024

Rhodopseudomonas capsulataserine-glycine biosynthesis, mutational analysis,

532Rhodospirillum tenue

lipophilic 0-antigens, 62916S ribonucleic acid

location between 30S and 50S ribosomes ofE. coli,900

Ribonucleic acid deficiencymethionyl transfer, 11S. cerevisiae, 11

Ribonucleic acid polymerasesuppression of the dnaA phenotype in S. typhimu-rium and E. coli, 577

Ribonucleic acid polymerasesHistoplasma capsulatum, inhibition by homolo-

gous and heterologous RNA, 1387Ribonucleoside diphosphate reductase synthesisE. coli, 107increased enzyme synthesis, 107

Ribonucleoside diphosphate reductase componentssynthesis in E. coli, 957

Ribosomal proteinsof E. coli, enzymatic methyl esterification, 839

Ribosomal ribonucleic acidchloramphenicol-induced changes in synthesis,

1098chloramphenicol treatment, 1109elongation in E. coli B/r, 1109synthesis in E. coli B/r, 1098

30S and 50S ribosomesnear location of 16S RNA, 900

risAaffecting plasmid maintenance in P. aeruginosa,

943R-plasmid-coded /3-lactamase

synthesis in minicells, 852R-plasmid deoxyribonucleic acid

differential association with a P. mirabilis lysate,1262

R-plasmid-mediated restriction-modification systemconferred by group E R plasmids on DNA, 529of DNA, 529

R-plasmidsderepressed, characterization in P. pseudomonas,

596rpoB cistron of ribonucleic acid polymerase

SUBJECT INDEX xi

E. coli, 577S. typhimurium, 577

Saccharomycespools of glycogen, 818

Saccharomyces carlsbergenesisfructose diphosphatase inactivation by yeast, 526

Saccharomyces cerevisiaeamino acid transport and metabolism, 714asparaginase II, 128effects of trifluperidol on the sterol, steryl ester,and fatty acid metabolism, 1310

fusion of spheroplasts, 946Gl arrest, 11genetic and physiological relationships between L-

asparaginase I and asparaginase II, 128glycolytic pathway mutants, isolation and charac-

terization, 746inositol starvation affecting growth and metabo-

lism, 472isolated mitochondria, 973L-asparaginase I, 128methionyl-transfer ribonucleic acid deficiency, 11nitrogen starved cells, 714ornithine carbamoyltransferase production, 1253replicative DNA synthesis, 973transcription level, ornithine carbamoyltransfer-

ase production, 1253Saccharomyces cerevisiae mating-type a cells

recovery from Gl arrest by a factor, 766Saccharomyces cerevisiae mutant

deficiency in pyruvate kinase activity, 232Saccharomycopsis lipolytica

neutral protease, 1125Salmonella species

sensitivity to colicin M and bacteriophages T5, Ti,and ES18, 1214

Salmonella typhimuriumchemotactic mechanism of mutants, 223enzyme synthesis, 983motility influenced by naturally occurring conju-

gative plasmid, 604periplasmic phosphatases, 399phosphatases and a cyclic phosphodiesterase, 411protease-deficient mutants, degradation ofE. coli

,8-galactosidase fragments, 347regulation of phosphatases and a cyclic phospho-

diesterase, 420suppression ofdnaA phenotype by rpoB mutations

of RNA polymerase, 577transcription of hut operons, 192

Schizosaccharomyces pombelysis, conjugation-induced, 512

Seleniumaffecting growth of M. vannielii, 1404

Serine-glycine biosynthesismutational analysis in R. capsulata, 532

Single-strand breaks in deoxyribonucleic acidrepair, 187

Small molecules and enzymesin the mother cell compartment and the forespore

of sporulating B. megaterium, 1130Somatic pilus mutants

genetic complementation analysis of E. coli type1, 506

xii SUBJECT INDEX

Spheroplastsfusion in S. cerevisiae, 946

Sphingolipid precursorsenergy-dependent incorporation in B. melanino-genicus, 445

Spirochaeta aurantiachemotaxis, 485

Sporogenesisof B. cereus, glutamate in, 242

Sporulating Bacillus megateriumsmall molecules and enzymes, 1130

Sporulationlecithin requirement in N. crassa, 524

Staphylococcus aureushexose and hexitol phosphoenol pyruvate-depend-

ent phosphotransferases, 991lysogenic conversion for multiple characters, 1281

Sterol metabolism of Saccharomyces cerevisiaeeffects of trifluperidol, 1310

Steryl ester metabolism ofSaccharomyces cerevisiaeeffects of trifluperidol, 1310

Streptococcus faecalisgenetic determinants on cryptic plasmids, 759plasmid DNA in a cariogenic strain, 759

Streptococcus faecalis 5952hemolysin and bacteriocin production, 948

Streptococcus faecalis subsp. zymogeneshemolysin production, 1064genetic loci, 1064

Streptococcus lactisinorganic salts resistance associated with a lac-

tose-fermenting plasmid, 257phosphoenolpyruvate and 2-phosphoglycerate asendogenous energy sources, 583

Streptococcus lactis cellseffects of potassium ions on electrical and pH gra-

dients, 1017Sucrose-fermenting strainplasmids ofE. coli, 1402

Sugar accumulationby starved cells of S. lactis, 583

2-Succinylbenzoaterequired by mutants of E. coli K-12 in menaqui-none biosynthesis, 1038

Sulfolobus acidocaldariusautotrophically grown, 1326heterotrophically grown, 1326lipid composition during heterotrophic and auto-

trophic growth, 1326Superoxide dismutase

in chemostat cultures of E. coli, 805Synthesis control of pyruvate kinase isozymes

in M. racemosus, 661Synthesis of lipid A fatty acyl moieties

relA gene control, 114

Thiamine monophosphate phosphohydrolaseM. denitrificans, 542

T0120 plasmidregulatory implications in P. putida, 1149spontaneously occurring mutant in P. putidaMT20, 1149

TOL plasmidsmolecular sizes and relationships, 1274Pseudomonas, 1274

tolF mutantsE. coli K-12, outer membrane protein alterations,

781tonB gene products

functional stability in E. coli, 750Tn3 elementfrequency and site specificity, 888translocation, 888

Transaminase C activitylacking in E. coli K-12 mutant, 566

Transcription termination factor Rhoamino acid transport in E. coli, 1024

Transductional analysisofE. coli 0111:B4, 656

Transfer ribonucleic acidchloramphenicol-induced changes in synthesis,

1098in vivo aminoacylation, 1091synthesis in E. coli B/r, 1098

Transfer ribonucleic acid biosynthetic enzymesynthesis, 635tRNA (m5U)nethyltransferase fromE. coli, 635

Transfer ribonucleic acid (m5U)methyltransferaseregulation of synthesis from E. coli, 635

Transfer ribonucleic acid specieslysine isoacceptance, 1091

Treponema pallidumcultured mammalian cells, 1333scanning electron microscopy, 1333

Trifluperidoleffects on metabolism of S. cerevisiae, 1310hypocholesteremic effects on S. cerevisiae, 1310sterol, steryl ester, and fatty acid metabolism of

S. cerevisiae, 1310Tryptophanase synthesisE. coli, 57unstable mutations that relieve catabolite repres-

sion, 57T-strain mycoplasma

U. urealyticum, 292T-strain mycoplasma

U. urealyticum, localization of enzymes, 297Tungsten

affecting growth of M. vannielii, 1404Type 1 pilus production

deficiency in phase variants and mutants of E.coli, 495

tyrBmapping of aminotransferase, 441

Unsaturated fatty acid mutantsN. crassa, 1144

Ureaplasma urealyticumcytoplasmic fractions, 1390localization of enzymes, 297morphology of organisms and colonies, 464urease, 1390

Ureaplasma urealyticum (T-strain mycoplasma)growth affected by carbon dioxide, urea, and am-monia, 292

Ureasecytoplasmic fractions, 1390U. urealyticum, 1390

Urea transportin C. reinhardi at low concentrations, 266

J. BACTERIOL.

VOL. 130, 1977 SUBJECT INDEX xiii

Vibrio costicolacell-bound cations, 698moderately halophilic, 698

Virus-like particlebiochemical and physiological studies, 1303yeast, 1303

Vitamin B6alternately required byE. coli K-12 mutant, 566

Wall polymersbiosynthesis in B. subtilis, 1055

Wild-type CloDF13 plasmidsmitomycin C, 846proteins synthesized from, 846

Xanthine dehydrogenase specificity typedistribution among bacteria, 1175

Xanthine oxidase specificity typedistribution among bacteria, 1175

Yeast virus-like particlebiochemical and physiological studies, 1303

INDEX TO DATE OF ISSUE

Date of Issue

27 April 197717 May 19779 June 1977

Month

AprilMayJune

Pages

1-575577-972973-1407

JOURNALOF

BACTERIOLOGY

VOLUME 130

WASHINGTON, D C 20006

1977

JOURNAL OF BACTERIOLOGYVOLUME 130 0 1977

EDITORIAL BOARD

L. Leon Campbell, Editor-in-Chief (1980)University of Delaware, Newark

S. G. Bradley, Editor (1979)Virginia Commonwealth University, Richmond

Elizabeth McFall, Editor (1980)New York University, New York, N.Y.

Allen T. Phillips, Editor (1980)Pennsylvania State University,

University Park, Pa.

Carl Schnaltman, Editor (1978)University of Virginia, Charlottesville

Simon Siver, Editor (1981)Washington University, St. Louis, Mo.

Mark Achtman (1979)James N. Adams (1979)Arthur I. Aronson (1979)Gad Avigad (1977)Stephen . Barbour (1979)Richard S. Berk (1977)Harriet Bernbeimer (1977)Judith S. Bond (1977)Winfried Boos (1979)H. D. Braymer (1979)Jean Brenchley (1977)George H. Brownell (1977)Richard 0. Burns (1977)Peter J. Chapman (1979)G. William Claus (1979)Don B. Clewell (1977)Stanely N. Cohen (1979)Ronald S. Cole (1977)S. F. Conti (1978)Stephen Cooper (1978)John E. Cronan, Jr. (1977)Walter J. Dobrogosz (1979)Patrick R. Dugan (1977)A. Eisenstark (1979)Alan D. Elbein (1979)Wolfgang Epstein (1978)David P. Fan (1978)Walton L. Fangman (1978)

Gerald R. Fink (1978)W. R. Finnerty (1979)Harry E. Gillebnd, Jr. (1979)Walter R. Guild (1978)Tadayo Hashimoto (1979)Gerald L. Hazelbauer (1978)George Hegeman (1977)James A. Hoch (1977)Joy Hochstadt (1977)Stanley C. Holt (1977)Philip Hylemon (1979)Joseph Inselburg (1978)Robert J. Kadner (1979)Sam Kaplan (1977)Jost Kemper (1978)David E. Kennel (1977)Bruce C. Kline (1977)Thomas G. Lessie (1979)Robert L. Lester (1978)Hillel S. Levinson (1978)K. Brooks Low (1979)Calvin S. McLaughlin (1978)P. T. Magee (1977)Michael H. Malamy (1978)Manley Mandel (1978)Robert E. Marquis (1979)Neil H. Mendelson (1978)Gene E. Michaels (1977)

Eugene W. Nester (1978)Donald P. Nierlich (1977)John H. Nordin (1979)Mlchio Olshi (1977)Ronald H. Olsen (1978)Sunil Palchaudhuri (1979)Charles Panos (1978)Leo Parks (1979)Martin Pato (1978)William S. Reznikoff (1979)Palmer Rogers (1978)Antonio H. Romano (1979)Robert Rownd (1977)Harold L. Sadoff (1977)Milton H. Saier, Jr. (1979)Gene A. Scarborough (1979)Irwin H. Segel (1979)Jane K. Setlow (1978)J. A. Shapiro (1979)Issar Smith (1978)Lucile Smith (1978)Dwight Stinnett (1979)Robert L. Switzer (1979)Bonnie M. Tyler (1978)James A. Wechsler (1978)David White (1977)Clifford A. Woolfolk (1979)Howard Zalkin (1979)

Robert A. Day, Managing EditorGisells Poiock, Assistant Managing Editor

Linda Mig, Production Editor1913 I St., N. W., Washington, D. C. 20006

EX OFFICIOHarlyn 0. Halvorson, President (1976-1977)

J. Mebsen Joseph, Secretary

The Journal of Bacteriology, a publication of the AmericanSociety for Microbiology, 1913 I St., N.W., Washington, D.C.20006, is devoted to the advancement and dissemination offundamental knowledge concerning bacteria and other micro-organisms. The Journal is published monthly, and the twelvenumbers are divided into four volumes per year. The non-member subscription price is $105 per year. Single copies are$9. The member subscription price is $21 per year. Corre-spondence relating to subscriptions, reprints, defective copies,availability of back issues, lost or late proofs, disposition of

A. Frederick Ramusse, Jr., Vice-President (1976-1977)Brinton M. Miller, Treasurer

submitted manuscripts, and general editorial matters shouldbe directed to the ASM Publications Office, 1913 I St., N.W.,Washington, D.C. 20006 (area 202 833-9680).

Second class postage paid at Washington, D. C. 20006, and atadditional mailing offices.Made in the United States of America.Copyright 0 1977, American Society for Microbiology.All Rights Reserved.

Volume 130 Contents for AprilMorphology and Ultrastructure

Negative Chemotaxis in Cellular Slime Molds. MARK T. KEATING AND J. T.BONNER* .............................................................. 144

Cell Surface Topography of Candida and Leucosporidium Yeasts as Revealed byScanning Electron Microscopy. KENNETH WATSON* AND HELEN ARTHUR 312

Cell Surface-Localized Alkaline Phosphatase of Escherichia coli as Visualized byReaction Product Deposition and FerTitin-Labeled Antibodies. T. J. MAC-ALISTER, R. T. IRVIN, AND J. W. COSTERTON* .... ........................ 318

Immunocytological Investigation of Protein Synthesis in Escherichia coli. T. J.MACALISTER, R. T. IRVIN, AND J. W. COSTERTON* .... .................... 329

Cell Envelope Protection of tlkaline Phosphatase Against Acid Denaturation inEscherichia coli. T. J. MACALISTER, R. T. IRVIN, AND J. W. COSTERTON* .. 339

Homogeneity of Lipopolysaccharides from Acholeplasma. PAUL F. SMITH ...... 393

Morphology of Ureaplasma urealyticum (T Mycoplasma) Organisms and Colo-nies. SHMUEL RAZIN, GERALD K. MASOVER,* MARINA PALANT, AND LEON-ARD HAYFLICK .......................................................... 464

Conjugation-Induced Lysis of Schizosaccharomyces pombe. G. B. CALLEJA,*BONG Y. Yoo, AND BYRON F. JOHNSON ..... ............................. 512

Inhibition of Germ Tube Formation in Neurospora. BRUCE M. EBERHART* .... 516

Lecithin Requirement for the Sporulation Process in Neurospora crassa. DAVORJURETI1 ............................................................... 524

Chemical Analysis of the Phenol-Water-Extractable Materials from Anabaena

flos-aquae. AUGUSTINE W. WANG* AND AUBREY HILL.................... 558

Genetics and Molecular BiologyBiochemical and Regulatory Properties of Escherichia coli K-12 hisT Mu-

tants. C. B. BRUNI, V. COLANTUONI, L. SBORDONE, R. CORTESE, AND F.BLASI* .............................................................. 4

Methionyl-Transfer Ribonucleic Acid Deficiency During Gi Arrest ofSaccharomy-ces cerevisiae. MICHAEL W. UNGER ..... ................................ 11

Unstable Mutations That Relieve Catabolite Repression ofTryptophanase Synthe-sis by Escherichia coli. M. D. YUDKIN ..... ............................ 57

Genetic Control of Arylsulfatase Synthesis in Klebsiella aerogenes. YOSHIKATSUMUROOKA,* TOSHIRO ADACHI, HARUKI OKAMURA, AND TOKUYA HARADA .... 74

Initiation and Termination of Deoxyribonucleic Acid Replication in Bacteria Aftera Stepwise Increase in the Velocity of Replication. H. BREMER,* R. YOUNG,AND G. CHURCHWARD.................................................. 92

Pseudoreversion of Lactose Operator-Constitutive Mutants. WILLIAM I. NOR-WOOD* AND JOHN R. SADLER ....... ..................................... 100

Regulation of Ribonucleoside Diphosphate Reductase Synthesis in Escherichiacoli: Increased Enzyme Synthesis as a Result of Inhibition of DeoxyribonucleicAcid Synthesis. DAVID FILPULA AND JAMES A. FUCHS* ... ............... 107

Medium-Dependent Variation of Deoxyribonucleic Acid Segregation in Esche-richia coli. STEPHEN COOPER* AND MARTIN WEINBERGER ................. 118

Genetic and Physiological Relationships Between L-Asparaginase I and Asparagi-nase II in Saccharomyces cerevisiae. GARY E. JONES ..................... 128

Method for Obtaining More-Accurate Covalently Closed Circular Plasmid-to-Chro-

* Asterisk refers to person to whom inquiries regarding the paper should be addressed.

xvii

Number 1

mosome Ratios from Bacterial Lysates by Dye-Buoyant Density Centrifuga-tion. DAVID D. WOMBLE, DEAN P. TAYLOR, AND ROBERT H. ROWND* ...... 148

Role of the 30S Ribosomal Subunit, Initiation Factors, and Specific Ion Concentra-tion in Barotolerant Protein Synthesis in Pseudomonas bathycetes. J. V.LANDAU,* W. P. SMITH, AND D. H. POPE ....... ......................... 154

Metabolic Characterization of the Viable, Residually Dividing and NondividingCell Classes of Recombination-Deficient Strains ofEscherichia coli. JUDITHE. MILLER* AND STEPHEN D. BARBOUR ........ .......................... 160

Method for the Isolation of Escherichia coli Mutants with Enhanced Recombina-tion Between Chromosomal Duplications. E. BRUCE KONRAD ............. 167

Repair of Hydrogen Peroxide-Induced Single-Strand Breaks in Escherichia coliDeoxyribonucleic Acid. H. N. ANANTHASWAMY AND A. EISENSTARK* ...... 187

Transcription of the hut Operons of Salmonella typhimurium. TERRANCE G.COOPER* AND BONNIE TYLER ........... ................................. 192

Physiological Regulation ofa Decontrolled lac Operon. BARRY L. WANNER, RYOJIKODAIRA, AND FREDERICK C. NEIDHARDT* ....... ......................... 212

Chemotactic Mechanism of Salmonella typhimurium: Preliminary Mapping andCharacterization of Mutants. H. M. WARRICK, BARRY L. TAYLOR, AND D. E.KOSHLAND, JR. * ...................................................... 223

Isolation and Characterization of a Saccharomyces cerevisiae Mutant Deficient inPyruvate Kinase Activity. GEORGE F. SPRAGUE, JR...................... 232

Inorganic Salts Resistance Associated with a Lactose-Fermenting Plasmid inStreptococcus lactis. J. D. EFSTATHIOU AND L. L. MCKAY* ..... .......... 257

Suppression of an Escherichia coli dnaA Mutation by the Integrated R FactorR100. 1: Generation of Small Plasmids After Integration. M. CHANDLER,* L.SILVER, J. FREY, AND L. CARO .......... ................................ 303

Degradation of Escherichia coli 8-Galactosidase Fragments in Protease-DeficientMutants of Salmonella typhimurium. CHARLES G. MILLER* AND DAVID ZIP-SER ................................................................... 347

Isolation and Characterization of Plaque-Forming XdnaZ+ Transducing Bacterio-phages. JAMES R. WALKER,* JOAN M. HENSON, AND CHONG S. LEE ....... 354

Escherichia coli Transport Mutants Lacking Binding Protein and Other Compo-nents of the Branched-Chain Amino Acid Transport Systems. JAMES J.ANDERSON AND DALE L. OXENDER* ......... ............................. 384

Escherichia coli Mutants Deficient in the Aspartate and Aromatic Amino AcidAminotransferases. DAVID H. GELFAND* AND ROBERT A. STEINBERG ...... 429

Mapping ofthe Aspartate and Aromatic Amino Acid Aminotransferase Genes tyrBand aspC. DAVID H. GELFAND* AND NAOMI RUDO ...... ................. 441

Isolation and Characterization of Escherichia coli Phase Variants and MutantsDeficient in Type 1 Pilus Production. LOIS M. SWANEY, YING-PING LIU,CHUEN-MO To, CHENG-CHIN To, KARIN IPPEN-IHLER, AND CHARLES C. BRIN-TON, JR.* ............................................................. 495

Genetic Complementation Analysis of Escherichia coli Type 1 Somatic PilusMutants. LOIS M. SWANEY, YING-PING LIU, KARIN IPPEN-IHLER, ANDCHARLES C. BRINTON, JR.* ............ ................................. 509

New R Plasmid-Mediated Restriction-Modification System of DeoxyribonucleicAcid Conferred by Group E R Plasmids. TOSHIHIKO ARAI AND TAKASHIAOKI* .............................................................. 529

Mutational Analysis of Serine-Glycine Biosynthesis in Rhodopseudomonas capsu-lata. PHILLIP D. BEREMAND AND GARY A. SOJKA* ..... .................. 532

Initiation and Termination of Chromosome Replication at 45°C in a Temperature-

x *ii CONTENTS

Sensitive Deoxyribonucleic Acid Initiation Mutant of Bacillus subtilis 168,TsB134. L. BURNETT AND R. G. WAKE*.... ............................. 538

Further Characterization of the Recipient Ability of Escherichia coli K-12 Bacte-riophage-Resistant Mutants. PAUL A. MANNING* AND PETER REEVES ..... 540

Biochemical Characterization of an Escherichia coli hisT Strain. ROBERT P.LAWTHER AND G. WESLEY HATFIELD*..... ............................... 552

Escherichia coli K-12 Mutant with Alternate Requirements for Vitamin B6 orBranched-Chain Amino Acids and Lacking Transaminase C Activ-ity. JOSEPH O. FALKINHAM III ......................................... 566

Physiology and MetabolismIsolation and Characterization of Lipopolysaccharides from Cell Walls of Blue-

Green Algae of the Genus Phormidium. L. V. MIKHEYSKAYA, R. G. Ovo-DOVA, AND Yu. S. OVODOV* ............................................. 1

Uptake of Ferrienterochelin by Escherichia coli: Energy-Dependent State of Up-take. ANTHONY P. PUGSLEY* AND PETER REEVES.... .................... 26

Third System for Neutral Amino Acid Transport in a Marine Pseudo-monad. SUSANNE M. PEARCE,* VIRGINIA A. HILDEBRANDT, AND THERESALEE............................................................ 37

Occurrence and Nature of Chromatic Adaptation in Cyanobacteria. N. TANDEAUDE MARSAC ............................................................ 82

relA Gene Control of the Synthesis of Lipid A Fatty Acyl Moieties. ALLENSPENCER, ERIC MULLER, JOHN E. CRONAN, JR.,* and TRACY A. GROSS...... 114

Formation and Dissimilation of Oxalacetate and Pyruvate in Pseudomonas citro-nellolis Grown on Noncarbohydrate Substrates. R. W. O'BRIEN* AND BARRYL. TAYLOR ........................................................... 131

Regulation of PRPP and Nucleoside Tri- and Tetraphosphate Pools in Escherichiacoli Under Conditions of Nitrogen Starvation. INGRID S. VILLADSEN* ANDOLE MICHELSEN ....................................................... 136

Specificity of Siderophore Receptors in Membrane Vesicles of Bacillus megate-rium. J. E. ASWELL, A. H. HAYDON, H. R. TURNER, C. A. DAWKINS, J. E. L.ARCENEAUX, AND B. R. BYERS* ......... ................................ 173

Cellulase of Neurospora crassa. BRUCE M. EBERHART,* RETA S. BECK, ANDKATHY M. GOOLSBY ..................................................... 181

Classification of Bacillus subtilis Flagellins. MELVIN I. SIMON, SUZANNE U.EMERSON, JOEL H. SHAPER, PATRICIA D. BERNARD, AND ALEXANDER N.GLAZER* ......................................................... 200

Extracellular Phosphatases of Chlamydomonas reinhardi and Their Regula-tion. N. J. PATNI, S. W. DHAWALE, AND S. AARONSON* .... .............. 205

Role of Glutamate in the Sporogenesis of Bacillus cereus. J. F. CHARBA* AND H.M. NAKATA ........................................................... 242

Transport of Urea at Low Concentrations in Chlamydomonas reinhardi. STUARTK. WILLIAMS II AND ROBERT C. HODSON* ....... ......................... 266

Cellular Distribution of Ornithine in Neurospora: Anabolic and Catabolic SteadyStates. BARRY J. BOWMAN* AND ROWLAND H. DAVIS ..... ............... 274

Arginine Catabolism in Neurospora: Cycling of Ornithine. BARRY J. BOWMAN*AND ROWLAND H. DAVIS ................................................ 285

Effects of Carbon Dioxide, Urea, and Ammonia on Growth of Ureaplasma urealyti-cum (T-Strain Mycoplasma). G. K. MASOVER,* S. RAZIN, AND L. HAYFLICK 292

Localization of Enzymes in Ureaplasma urealyticum (T-Strain Mycoplasma). G.K. MASOVER,* S. RAZIN, AND L. HAYFLICK ....... ........................ 297

XiXCONTENTS

Klebsiella Serotype 25 Capsular Polysaccharide: Primary Structure and Depolym-erization by a Bacteriophage-Borne Glycanase. HEINER NIEMANN,* BAR-TTOMIEJ KWIATOWSKI, ULRICH WESTPAHL, AND STEPHAN STIRM .... ........ 366

Energy-Dependent Incorporation of Sphingolipid Precursors and Fatty Acids inBacteroides melaninogenicus. MEIR LEV* AND ALBERT F. MILFORD ....... 445

Regulation of Galactose Oxidase Synthesis and Secretion in Dactylium dendroides:Effects of pH and Culture Density. ALLAN R. SHATZMAN AND DANIEL J.KOSMAN* ............................................................. 455

Growth and Metabolism of Inositol-Starved Saccharomyces cerevisiae. SUSAN A.HENRY,* KATHARINE D. ATKINSON, ANITA I. KOLAT, AND MICHAEL R. CUL-BERTSON .............................................................. 472

Chemotaxis in Spirochaeta aurantia. E. P. GREENBERG AND E. CANALE-PAROLA* 485Efficiency of Oxidative Phosphorylation in Continuous Cultures ofCandida parap-

silosis. P. J. ROGERS ...................................................521Phospholipid and Fatty Acid Composition of Methanol-Utilizing Bacteria. I.

GOLDBERG* AND A. P. JENSEN ................................ 535Hydrolysis of Lithocholate Sulfate by Pseudomonas aeruginosa. T. J. IMPER-

ATO,* C. G. WONG, L. J. CHEN, AND R. J.' BOLT ...... .................... 545Ethylene-Induced Growth in Phycomyces Mutants Abnormal for Autochemotro-

pism. V. E. A. Russo ............... .................................. 548Effect of Temperature on the Growth of Myxococcus xanthus. GARY R. JANSSEN,

JOHN W. WIREMAN, AND MARTIN DWORKIN* ...... ....................... 561Differential Binding of Cyclic Adenosine 3',5'-Monophosphate to the Cyclic Adeno-

sine 3',5'-Monophosphate Receptor Protein in Escherichia coli. DENNIS E.DANLEY, MARK DREXLER, AND J. L. BOTSFORD* ...... ..................... 563

Metabolic Role of Free Mycolic Acids in Mycobacterium tuberculosis. KUNI TAK-AYAMA* AND EMMA LEE ARMSTRONG ........ ............................ 569

Choline Metabolism in Pneumococci. BARRY BEAN* AND ALEXANDER TOMASZ.. 571

EnzymologyPhosphoribulokinase from Nitrobacter winogradskyi: Activation by Reduced Nico-

tinamide Adenine Dinucleotide and Inhibition by Pyridoxal Phos-phate. LUTZ A. KIESOW,* BENJAMIN F. LINDSLEY, AND JUERGEN W. BLESS 20

Extracellular Acid Protease of Aspergillus oryzae Grown on Liquid Media: Multi-ple Forms due to Association with Heterogeneous Polysaccharides. YOSHIOTSUJITA AND AKIRA ENDO* ........... .................................. 48

O-Alkylhomoserine Synthesis Catalyzed by O-Acetylhomoserine Sulfhydrylase inMicroorganisms. YOSHIKATSU MUROOKA,* KATSUSHI KAKIHARA, TOSHIAKIMIWA, KOJI SETO, AND TOKUYA HARADA ....... ......................... 62

Alkaline Phosphatase ofBlastocladiella emersonii: Partial Purification and Char-acterization. CLAUDE P. SELITRENNIKOFF* AND DAVID R. SONNEBORN ..... 249

Characterization of the Entomocidal Parasporal Crystal of Bacillus thuringien-sis. LEE A. BULLA, JR.,* KARL J. KRAMER, AND LOREN I. DAVIDSON ...... 375

Resolution and Purification of Three Periplasmic Phosphatases of Salmonellatyphimurium. LARRY D. KIER, ROGER WEPPELMAN, AND BRUCE N. AMES* 399

Properties of Two Phosphatases and a Cyclic Phosphodiesterase of Salmonellatyphimurium. ROGER WEPPELMAN, LARRY D. KIER, AND BRUCE N. AMES* 411

Regulation of Two Phosphatases and a Cyclic Phosphodiesterase of Salmonellatyphimurium. LARRY D. KIER, ROGER WEPPELMAN, AND BRUCE N. AMES* 420

Inactivation of Fructose Diphosphatase by Sucrose in Yeast. D. H. J. SCHAM-HART, M. P. M. VAN DEN HEIJKANT, AND K. W. VAN DE POLL* ............. 526

xx CONTENTS

Specific Thiamine Monophosphate Phosphohydrolase in Micrococcus denitrifi-cans. TAKASHI KAWASAKI,* YOSHIKo EGI, AND HIROSHI SANEMORI ........ 542

Erratum3-Deoxy-3-Fluoro-D-Glucose-Resistant Salmonella typhimurium Mutants Defec-

tive in the Phosphoenolpyruvate:Glycose Phosphotransferase Sys-tem. THOYD MELTON, WERNER KUNDIG, PHILIP E. HARTMAN,* AND NORMANMEADOW .............................................................. 575

Volume 130 Contents for May Number 2Morphology and Ultrastructure

Flagellar Formation in Escherichia coli Electron Transport Mutants. J. BARTANA, BARBARA J. HOWLETT, AND D. E. KOHSLAND, JR.* ..... ............ 787

Polysaccharide Capsule of Escherichia coli: Microscope Study of Its Size, Struc-ture, and Sites of Synthesis. MANFRED E. BAYER* AND HORST THUROW ... 911

Gliding Motility of Mycoplasma pulmonis. W. BREDT* AND U. RADESTOCK ..... 937

Genetics and Molecular BiologySuppression of the DnaA Phenotype by Mutations in the rpoB Cistron of Ribonu-

cleic Acid Polymerase in Salmonella typhimurium and Escherichiacoli. MIROSZAWA M. BAGDASARIAN, MARYLA IZAKOWSKA, AND MICHAELBAGDASARIAN* ........................................................ 577

Characterization of Pseudomonas aeruginosa Derepressed R-Plasmids. P. M.CHANDLER AND V. KRISHNAPILLAI* ......... ............................. 596

Influence on Motility of Escherichia coli and Salmonella typhimurium by aNaturally Occurring Conjugative Plasmid. THOMAS BOHLIN AND LARS G.BURMAN* ............................................................. 604

Stringent Regulation of the Synthesis of a Transfer Ribonucleic Acid BiosyntheticEnzyme: Transfer Ribonucleic Acid(m5U)methyltransferase from Escherichiacoli. TOR NY AND GLENN R. BJORK* ........ ............................ 635

In Vitro Transcription of the Escherichia coli argA, argE, and argCBH Op-erons. DON SENS, WILLIAM NATTER, AND ERIC JAMES* ..... ............. 642

Genetic Analysis ofEscherichia coli O111:B4, a Strain of Medical and BiochemicalInterest. WILLIAM G. COLEMAN, JR.,* PAUL J. GOEBEL, AND LORRETA LEIVE 656

Deoxyribonucleic Acid Repair in Escherichia coli Mutants Deficient in the 5' -- 3'Exonuclease Activity of Deoxyribonucleic Acid Polymerase I and ExonucleaseVII. JOHN W. CHASE* AND WARREN E. MASKER ...... ................... 667

Mutations in the L-Arabinose Operon ofEscherichia coli B/r with Reduced Initia-tor Function. IRIS LAUDIEN GONZALEZ AND DAVID E. SHEPPARD* .... ..... 684

Deoxyribonucleic Acid Synthesis After Inhibition of Initiation of Rounds of Repli-cation in Escherichia coli B/r. HANS BREMER* AND GORDON CHURCHWARD 692

Isolation and Characterization of Nuclei from Neurospora crassa. JUDITH A.HAUTALA, BARBARA H. CONNER, JAMES W. JACOBSON, GORDHAN L. PATEL,AND N. H. GILES*...................................................... 704

Cell Division in Escherichia coli B,12 Is Hypersensitive to Deoxyribonucleic AcidDamage by Ultraviolet Light. B. A. BRIDGES,* R. P. MOTTERSHEAD, AND M.H. L. GREEN .......................................................... 724

Isolation and Characterization of Nondefective Transducing Lambda Bacterio-phages Carrying fla Genes ofEscherichia coli K-12. HISATO KONDOH ..... 736

XXiCONTENTS

Isolation and Characterization of Saccharomyces cerevisiae Glycolytic PathwayMutants. KENG-BON LAM* AND JULIUS MARMUR.............746

Analysis of Plasmid Deoxyribonucleic Acid in a Cariogenic Strain ofStreptococcusfaecalis: an Approach to Identifying Genetic Determinants on Cryptic Plas-mids. DAPHNA R. OLIVER, BRYRON L. BROWN, AND DON B. CLEWELL* .... 759

Recovery of Saccharomlyces cerevisiae Mating-Type a Cells from Gl Arrest by aFactor. RuSSELL K. CHAN........................766

Regulatory Changes in the Fucose System Associated with the Evolution of aCatabolic Pathway for Propanediol in Escherichia coli. ANDREW J. HACKINGAND E. C. C. LIN*............................832

Changes in Protei S thesis on Mitomycin C Induction of Wild-Type and MutantCoDF13 Pasmids G. DOUGAN AND D. J. SHERRATT*...........846

Synthesis of R-Plasmid-Coded j3-Lactamase in Minicells and in an In Vitro Sys-tem. JUNE N. GRINDLEY, RICHARD BLUMBERG, AND DAi NAKADA* .....852

Analysis ofEuglena gracilis Chloroplast Deoxyribonucleic Acid with a RestrictionEndonuclease, EcoRI. JONATHAN R. MIELENZ,* JOEL J. MILNER, ANDCHARLEs L. HERSHBERGER........................860

Characterization of Lambda Escherichia coli Hybrids Carrying7 ChemotaxisGenes. M. SILVERMAN, P. MATSUMURA, J. HILMEN, AND M. AIMON* ....877

Selected Translocation of Plasmid Genes: Frequency and Regional Specificity ofTranslocation of the Tn3 Element. PETER J. KRETSCHMER AND STANLEY N.COHEN*.................................888

Area of 1&S Ribonucleic Acid at or Near the Interface Between 305 and 50SRibosomes of Escherichia coli. MELVIN SANTER* AND SARA SHANE.....900

Stepwise Selection of Defective Nitrogen-Fixing Phenotypes in Escherichia coli K-12 by Dimethyl Sulfoxide. MARY L. SKoTNICKI AND BARRY G. RouLFE*....939

Bacterial Mutation Affecting Plasmid Maintenance in Pseudomonas aerugi-nosa. B. J. CHANG AND B. W. HOLLOWAY*................943

Fusion of Yeast Spheroplasts. PIETER VAN SOLINGEN AND JOHANNEs B. VAN DERPLAAT*.................................946

Characterization of Plasmids Determnining Hemolysin and Bacteriocin Productionin Streptococcus faecalis 5952. DAPHNA R. OLIVER, BYRON L. BROWN, ANDDON B. CLEWELL*............................948

Genetic Analysis of Azotobacter vinelandii Mutant Strains Unable to Fix Nitro-gen. PAuL E. BISHOP AND. WINSTON J. BRILL*...............954

Dominance ofdnaA + to dnaA inEscherichia coli. FERN GOTFRIED AND JAMEs A.WECHSLER*...............................963

Physiology and MetabolismPhosphoenolypyruvate and 2-Phosphoglycerate: Endogenous Energy Source(s) for

Sugar Accumulation by Starved Cells of Streptococcus lactis. JOHN THOMP-SON* AND TERENCE D. THomAs......................583

Relation Between Cell Wall Turnover and Cell Growth in Bacillus subtilis. LuisGLASER* AND BARBARA LINDSAY .....................610

Phenethyl Alcohol Inhibition ofs-lcrl3Phosphate Acylation in Escherichiacoli. WILLIAm D. NUNN* P0JNCEG, ROBERT DEUTSCH, CHu T. TANG,AND BURTON E. TROPP..........................620

Lipophilic 0-Antigens in Rhodospirillum tenue. J. WECKESSER,* G. DREWS, R.INDIRA, AND H. MAYER..........................629

Pyruvate Kinase Isozymes of Mucor racemosus: Control of Synthesis by Glu-cose. JOHN L. PAZNOKAS* AND PAUL S. SYPHERD .............661

xxii CONTENTS

Functional Capacities and the Adenylate Energy Charge inEscherichia coli UnderConditions of Nutritional Stress. MARY WALKER-SIMMONS AND DANIEL E.ATKINSON* ............................................................. 676

Cell-Bound Cations of the Moderately Halophilic Bacterium Vibrio costicola. D.B. SHINDLER, R. M. WYDRO, AND D. J. KUSHNER* ..... ................... 698

Amino Acid Transport and Metabolism in Nitrogen-Starved Cells of Saccharomy-ces cerevisiae. JOHN R. WOODWARD AND VINCENT P. CIRILLO* .... ........ 714

Different Binding Sites for Entry and Exit of Amino Acids in Whole Cells ofMycobacterium phlei. EITAN ISRAELI, VIJAY K. KALRA, AND ARNOLD F.BRODIE* .............................................................. 729

Functional Stability of the bfe and tonB Gene Products in Escherichiacoli. PHILIP J. BASSFORD, JR., CARL A. SCHNAITMAN, AND ROBERT J. KAD-NER* .............................................................. 750

Iodination ofEscherichia coli with Chloramine T: Selective Labeling of the OuterMembrane Lipoprotein. ROBERT S. MUNFORD* AND EMIL C. GoTsCHILCH .. 775

Escherichia coli K-12 tolF Mutants: Alterations in Protein Composition of theOuter Membrane. TUU-JYI CHAI AND JOHN FOULDS* .... ................ 781

Regulation of Accumulation and Turnover of an Inducible Glutamate Dehydrogen-ase in Synchronous Cultures of Chlorella. DANIEL W. ISRAEL, RICHARD M.GRONOSTAJSKI, ANTHONY T. YEUNG, AND ROBERT R. SCHMIDT* ............ 793

Physiological Function of Superoxide Dismutase in Glucose-Limited ChemostatCultures ofEscherichia coli. H. MOUSTAFA HASSAN AND IRWIN FRIDOVICH* 805

Control of -Glucosidase Synthesis in Mucor racemosus. PETER BORGIA* ANDPAUL S. SYPHERD...................................................... 812

Two Pools of Glycogen in Saccharomyces. ZEENAT GUNJA-SMITH, NARAYAN B.PATIL, AND ERIC E. SMITH* ............................................. 818

Glycine Metabolism by Pseudomonas aeruginosa: Hydrogen Cyanide Biosyn-thesis. PETER A. CASTRIC.............................................. 826

Enzymatic Methyl Esterification of Escherichia coli Ribosomal Pro-teins. SANGDUK KIM, BETTY LEW, AND F. N. CHANG*... ................ 839

Multivalent Regulation of Isoleucine-Valine Transaminase in an Escherichia coliK-12 ilvA Deletion Strain. ELLis L. KLINE,* DELBERT N. MANROSS, JR., ANDMARY L. WARWICK .................................................... 951

Coordinate Control of the Synthesis of Ribonucleoside Diphosphate ReductaseComponents of Escherichia coli. JAMES A. FUCHS... .................... 957

Nucleoside Triphosphate Pools in Minicells of Escherichia coli. JOHN D. MAN-WARING AND JAMES A. FUCHS*.......................................... 960

Hemin-Dependent Growth Stimulation and Cytochrome Synthesis in Corynebacte-rium pyogenes. C. ADINARAYANA REDDY,* C. P. CORNELL, AND M. KAO ... 965

Transport of 3,4-Dihydroxybut1yl-1-Phosphonate, an Analogue of sn-Glycerol 3-Phosphate. ZEV LEIFER,ROBERT ENGEL, AND BURTON E. TROPP* ......... 968

EnzymologyIsolation and Characterization of a Polynucleotide Phosphorylase from Bacillus

amyloliquefaciens. ROBERT J. ERICKSON* AND JOSEPHINE C. GROSCH...... 869Errata

Relationships Between Cotransducible Plasmids in Staphylococcus au-reus. SERBAN IORDXNESCU............................................. 972

Role of a Sugar-Lipid Intermediate in Colanic Acid Synthesis by Escherichiacoli. JAMES G. JOHNSON AND DAVID B. WILSON*........................ 972

xxiiiCONTENTS

xxiv CONTENTS

Genetic Characterization of the Temperature-Sensitive and Suppression Pheno-types of Escherichia coli Mutant N4316. M. B. HERRINGTON AND M. C.GANOZA*....... ........ 972

Volume 130 Contents for June Number 3

Morphology and UltrastructureEffect of Macromolecular Synthesis on the Coordinate Morphogenesis of Polar

Surface Structures in Caulobacter crescentus. AKIo FUKUDA* AND YOSHIMIOKADA ................................................................ 1199

Biochemical and Physiological Studies of the Yeast Virus-Like Particle. S. G.OLIVER, S. J. MCCREADY, C. HOLM, P. A. SUTHERLAND, C. S. MCLAUGHLIN,*AND B. S. Cox. 1303

Comparative Lipid Composition of Heterotrophically and Autotrophically GrownSulfolobus acidocaldarius. THOMAS A. LANGWORTHY. 1326

Scanning Electron Microscopy of Treponema pallidum (Nichols Strain) Attachedto Cultured Mammalian Cells. T. J. FITZGERALD,* P. CLEVELAND, R. C.JOHNSON, J. N. MILLER, AND J. A. SYKES. 1333

Electron Microscopic Study of Cell Surface Rings During Cell Division and Mor-phogenesis ofArthrobacter crystallopoietes. P. E. KOLENBRANDER* AND . J.HOHMAN... 1345

Methanococcus vannielii: Ultrastructure and Sensitivity to Detergents and Anti-biotics. JAY B. JONES, BLAIR BOWERS, AND THRESSA C. STADTMAN* ... 1357

Effect of Ethylenediaminetetraacetate upon the Surface of Escherichia coli.MANFRED E. BAYER* AND LORETTA LEIVE. 1364

Influence of Oxygen on Phospholipid Production and Colony Formation in a

Nitrogen-Fixing Mutant ofAzobacter vinelandii. S. P. SCHENK AND ORVILLEWYss*. 1382

Genetics and Molecular BiologyRegulation of Enzyme Synthesis by the Glutamine Synthetase of Salmonella

typhimurium: a Factor in Addition to Glutamine Synthetase Is Required forActivation of Enzyme Formation. FREDRIC R. BLOOM, STANLEY L.STREICHER, AND BONNIE TYLER*. 983

Mode of Degradation of Precursor-Specific Ribonucleic Acid Fragments by Bacillussubtilis. ELLEN SCHROEDER, JANET MCKIBBIN, MITCHELL L. SOGIN, ANDNORMAN R. PACE*. 1000

Regulation ofAmino Acid Transport in Escherichia coli by Transcription Termina-tion Factor Rho. STEVEN C. QUAY AND DALE L. OXENDER*... 1024

Initiation of Deoxyribonucleic Acid Replication in Germinating Spores of Bacillussubtilis 168 Carrying the dnaB(Ts)134 Mutation. HEATHER CALLISTER, SUELE MESURIER, AND R. G. WAKE*. 1030

Menaquinone Biosynthesis: Mutants of Escherichia coli K-12 Requiring 2-Succi-nylbenzoate. JOHN R. GUEST. 1038

Genetic Loci of Hemolysin Production in Streptococcus faecalis subsp. zymo-genes. MARSHA L. FRAZIER AND LEONARD N. ZIMMERMAN*... 1064

In Vivo Aminoacylation of Transfer Ribonucleic Acid in Bacillus subtilis andEvidence for Differential Utilization of Lysine-Isoaccepting Transfer Ribonu-cleic Acid Species. JUDITH TOCKMAN AND BARBARA S. VOLD*.1091

Chloramphenicol-Induced Changes in the Synthesis of Ribosomal, Transfer, and

Messenger Ribonucleic Acids in Escherichia coli B/r. V. SHEN AND H. BRE-MER* ................................................................ 1098

Rate of Ribosomal Ribonucleic Acid Chain Elongation in Escherichia coli B/rDuring Chloramphenicol Treatment. V. SHEN AND H. BREMER* .... ...... 1109

Characterization of a Spontaneously Occurring Mutant of the TOL20 Plasmid inPseudomonas putida MT20: Possible Regulatory Implications. MICHAEL J.WORSEY AND PETER A. WILLIAMS* ....................................... 1149

Characterization and In Vitro Translation of Polyadenylated Messenger Ribonu-cleic Acid from Neurospora crassa. M. CRAN LUCAS, JAMES W. JACOBSON,AND NORMAN H. GILEs* ............ .................................... 1192

Determination of Deoxyribonucleic Acid Replication Time in Exponentially Grow-ing Escherichia coli B/r. GORDON CHURCHWARD AND HANS BREMER* ...... 1206

Genetics of Sensitivity of Salmonella Species to Colicin M and Bacteriophages T5,Ti, and ES18. AMY C. GRAHAM AND B. A. D. STOCKER* ..... ............ 1214

Altered Accumulation of a Membrane Protein Unique to a Membrane-Deoxyribo-nucleic Acid Complex in a dna Initiation Mutant of Bacillus sub-tilis. JEFFREY M. HARMON AND HARRY W. TABER* ..... ................. 1224

Differential Association of F' Plasmid and R Plasmid Deoxyribonucleic Acid with aRapidly Sedimenting Fraction of a Proteus mirabilis Lysate. LORNE TAICH-MAN AND ROBERT H. RoWND* .......... ................................. 1262

Molecular Sizes and Relationships of TOL Plasmids in Pseudomonas. CLIVE J.DUGGLEBY, SUSAN A. BAYLEY, MICHAEL J. WORSEY, PETER A. WILLIAMS, ANDPAUL BRODA*.......................................................... 1274

Lysogenic Conversion for Multiple Characters in a Strain of Staphylococcus au-reus. YVONNE DUVAL-IFLAH,* JEAN VAN HEIJENOORT, MICHELINE Rous-SEAU, AND PIERRE RAIBAUD............................................. 1281

Identification of Polypeptides Necessary for Chemotaxis in Escherichia coli. M.SILVERMAN AND M. SIMON* .......... .................................. 1317

Inhibition of Histoplasma capsulatum Ribonucleic Acid Polymerases by Homolo-gous and Heterologous Ribonucleic Acid. RAY MCMILLIAN,* B. VIJAY Ku-MAR, GERALD MEDOFF, DAVID SCHLESSINGER, AND G. S. KOBAYASHI .... .... 1387

Deoxyribonucleic Acid Strand Breaks During Freeze-Drying and Their Repair inEscherichia coli. TAKEO OHNISHI, YOSHINORI TANAKA, MYONSUN YOH,YOSHIFUMI TAKEDA, AND TOSHIO MIWATANI* ............................. 1393

Characterization of Plasmids in a Sucrose-Fermenting Strain of Escherichiacoli. SUNIL PALCHAUDHURI, SHELLEY RAHN, DIOGENES S. SANTOS, AND WER-NER K. MAAS* ......................................................... 1402

Physiology and MetabolismReplicative Deoxyribonucleic Acid Synthesis in Isolated Mitochondria from Sac-

charomyces cerevisiae. JOHN S. MATTICK AND RUTH M. HALL* .... ....... 973Initial Characterization of Hexose and Hexitol Phosphoenolpyruvate-Dependent

Phosphotransferases of Staphylococcus aureus. STANLEY A. FRIEDMAN ANDJOHN B. HAYS* ........................................................ 991

Oxygen-Dependent Inactivation of Gramicidin S Synthetase in Bacillusbrevis. T. E. FRIEBEL AND A. L. DEMAIN* .............................. 1010

Effects of Potassium Ions on the Electrical and pH Gradients Across the Membraneof Streptococcus lactis Cells. EVA R. KASHKET* AND SUSAN L. BARKER .... 1017

Pathways of Nucleotide Biosynthesis in Mycoplasma mycoides subsp. my-coides. ALANA MITCHELL AND LLOYD R. FINCH* ...... .................... 1047

Biosynthesis of Wall Polymers in Bacillus subtilis. ANNE W. WYKE AND J.BARRIE WARD*........................................................ 1055

CONTENTS xxv

A Four-Iron, Four-Sulfide Ferredoxin with High Thermostability from Clostrid-ium thermoaceticum. SHIOW-SHONG YANG, LARS G. LJUNGDAHL,* AND JEANLEGALL.................................................................1084

Levels of Small Molecules and Enzymes in the Mother Cell Compartment and theForespore of Sporulating Bacillus megaterium. RAVENDRA P. SINGH, BAR-BARA SETLOW, AND PETER SETLOW* ........ ............................. 1130

Unsaturated Fatty Acid Mutants of Neurospora crassa. WILLIAM A. ScoTT .... 1144Energy Requirements for the Transport of Methylthio-,3-D-Galactoside by Esche-

richia coli: Measurement by Microcalorimetry and by Rates of Oxygen Con-sumption and Carbon Dioxide Production. RICHARD A. LONG, W. G. MAR-TIN, AND HENRY SCHNEIDER* ......... .................................. 1159

Distribution of Xanthine Oxidase and Xanthine Dehydrogenase Specificity TypesAmong Bacteria. C. A. WOOLFOLK* AND J. S. DOWNARD ..... ............ 1175

Properties of an Escherichia coli K-12 Mutant Defective in the Transport ofArginine and Ornithine. T. F. R. CELIS . ............................... 1234

Independent Regulation of Transport and Biosynthesis of Arginine in Escherichiacoli K-12. T. F. R. CELIS .............................................. 1244

Evidence That Specific and "General" Control of Ornithine CarbamoyltransferaseProduction Occurs at the Level of Transcription in Saccharomyces cerevi-siae. FRANCINE MESSENGUY AND TERRANCE G. COOPER* .... ............. 1253

Basis for the Observed Fluctuationof Carboxypeptidase II Activity During the CellCycle in BUG 6, a Temperature-Sensitive Division Mutant ofEscherichia coliK-12. BARBARA D. BECK AND JAMES T. PARK* ..... ...................... 1292

Effects of the Hypocholesteremic Agent Trifluperidol on the Sterol, Steryl Ester,and Fatty Acid Metabolism of Saccharomyces cerevisiae. M. T. SOBUS, C. E.HOLMLUND,* AND N. F. WHITTAKER ....... .............................. 1310

Control of Arylsulfatase in a Serine Auxotroph of Neurospora. ERIC S. JACOB-SON* AND ROBERT L. METZENBERG ...... ................................ 1397

Normal Iron-Enterochelin Uptake in Mutants Lacking the Colicin I Outer Mem-brane Receptor Protein of Escherichia coli. S. SOUCEK AND J. KONIsKY* .. 1399

Methanococcus vannielii: Culture and Effects of Selenium and Tungsten onGrowth. JAY B. JONES AND THRESSA C. STADTMAN* .... ................. 1404

EnzymologyPartial Purification of Glycerophosphate Acyltransferase from Escherichia

coli. MARTIN D. SNIDER* AND EUGENE P. KENNEDY.... ................. 1072Purification and Properties of cis-Toluene Dihydrodiol Dehydrogenase from Pseu-

domonas putida. J. E. ROGERS* AND D. T. GIBSON .... .................. 1117Purification and Characterization of a Neutral Protease from Saccharomycopis

lipolytica. AHMED T. H. ABDELAL,* EMILY H. KENNEDY, AND DONALD G.AHEARN.............................................................. 1125

6-Phospho-D-Gluconate:NAD+ 2-Oxidoreductase (Decarboxylating) from Slow-Growing Rhizobia. G. MARTINEZ-DRETS,* A. GARDIOL, AND A. ARIAS ...... 1139

Multiple Forms of Urease in Cytoplasmic Fractions of Ureaplasma urealyti-cum. GLORIA J. DELISLE.............................................. 1390

ErrataRate of Ribonucleic Acid Chain Growth in Mycobacterium tuberculosis H37R . R.

M. HARSHEY AND T. RAMAKRISHNAN*.... ............................... 1407Complexity of the Ultraviolet Mutation Frequency Response Curve in Escherichia

coli B/r: SOS Induction, One-Lesion and Two-Lesion Mutagenesis. CHARLES0. DOUDNEY .......................................................... 1407

XXVi CONTENTS

INDEX TO ADVERTISERSPage

Academic Press, Inc.......................... 11Blaw Knox Food & Chemical ....................... ...............Cover 3

* Difco Laboratories ..................................................... Cover 4* Gelman Instrument Co................................Cover 2ISCO ........................... 10

* Pharmacia Fine Chemicals, Inc............................7, 9RoyalScientific.8.., , , , . .................................. 8

* Sustaining Member, American Society for Microbiology.

Advertising in this journal is limited to products and services believed to be of interest to the readers. However, ASMdoes not test nor examine advertised products nor claims related thereto. Therefore, ASM endorsement or approval ofadvertised products should not be inferred.

Trouble-Freem~~

Agituition 5;For continuous flask agitation of liquid or semi-liquid products, the rugged Gump Rotary FlaskAgitators are without equal. Available in one-, two- or three-tiered models, they aredimensionally compatible with your present set-up and can accommodate yourexisting trays. Applicable wherever there are exacting requirements for consistent,continuous agitation, the Gump agitators are engineered and built for endurancewhile variable speed drives permit optimum control of time/rotation cycles to

meet and maintain product standards.

One of the White Consolidated Industries DIVIS/ON BLAW-KNOX FOOD & CHEMICAL EQUIPMENT, INC.lXM@D BOX 1041. BUFFALO. NEW YORK 14240 PHONE. 716. 895-2100