Is there genec resistance of Nellore cale to both cks and ... · Gene*c varia*on was found to be...
Transcript of Is there genec resistance of Nellore cale to both cks and ... · Gene*c varia*on was found to be...
Is there gene*c resistance of Nellore ca2le to both *cks and gastro-intes*nal parasites?
FabioLuizBuraneloToralDepartmentofAnimalScience/UFMG
AndreaWilson’sgroup
GeneAcsandGenomicsDivision/Roslin
13thMeeAngEdinburghAllianceforComplexTraitGeneAcs
RoyalSocietyofEdinburgh
Brazilian ca2le
• 210-220Mheads
• ~80%beefcaQle• 168–176Mheads
• ~70%Zebubreeds• ~75%Nelloreorcrossbred
• 88–92MNelloreorcrossbred
Nellore is widely distributed over Brazil
PonteseLacerda,MT CarlosChagas,MG
Nellore is exposed to great seasonal varia*on
NeloreLemgruber NeloreIRCA
Animals should stay healthy along the year
Toreproduce Togrowth
ABSPecplanDanielResende
To achieve sustainability in livestock produc*on • Animalbreeding• Sanity• NutriAon• Animalwelfare• Etc…
The annual cost with an*parasi*c for ruminants in Brazil – 2016
R$852.5M £198.2M
AdaptedfromSINDAN(2017).
Animal breeding is about to explore differences between and within breeds
Selec:onCrossbreeding
Significant difference between gene*c groups for *ck counts
AdaptedfromIbellietal.(2012).
Non significant difference between gene*c groups for number of eggs per gram
Oliveiraetal.(2013).
Non significant effect of gastrointes*nal nematodes on Nellore growth Meannumberofnematodesintheresistant(n=10)andsuscep:ble(n=10)groupsofNelloreyoungbullsnaturallyinfectedbygastrointes:nalnematodes
Bodyweightoftheresistant(n=10)andsuscep:ble(n=10)groupsofNelloreyoungbulls,naturallyinfectedwithgastrointes:nalnematodes
Nematodes Resistant Suscep:ble Significance
Haemonchusspp. 156 1,272 <0.01
Cooperiaspp. 1,500 4,807 <0.05
Oesophagostomumspp. 100 174
Trichurisspp. 22 22
Total 1,778 6,275 <0.05
AdaptedfromBricarelloetal.(2007).
Non significant effect of *ck and gastrointes*nal nematodes on Nellore growth Group N Mean(g/day) SE(g/day)
Tick
High 533 557.37 3.24
Low 568 552.17 3.12
Nematodes
High 520 553.28 3.32
Low 581 555.84 3.12
Barbosa(2016).
Do *cks and nematodes affect Nellore performance?
• Yes.So,whytheresearchersdidn’tfindthis?
• No.So,whythefarmersspendsomuchmoneyonparasitetreatments?
Increase food safety in beef
• GeneAcvariaAonforresistancetoparasites(Acks,nematodesandEimeriaspp.)
• SelecAonasacomplementarytoolinparasitecontrolprograms
• ReducetheuAlizaAonofchemicalsinbeefcaQleproducAonsystems
Data recording in a Nellore seedstock farm
• Youngbullsonpastureperformancetests(294d)• Groupsof30-35animals(8groups/year)• 70dofadaptaAon• EvaluaAons(weightandparasitecounts)every56d,unAl550daysofage• Upto5measurements/animal
• FromApril2011toMay2017• 7,871Ackcounts(1,713animals,130sires)• 7,292nematodesandEimeriaspp.counts(1,712animals,130sires)• 5,920animalsinthepedigree
Distribu*ons of parasite counts are neither Gaussian nor Poisson
FiPng of Zero Inflated Poisson distribu*ons
• Tickcountindairycows(Silvaetal.,2011)
• Tickcountinsheep(Sae-Limetal.,2017)
• Faecaleggcountinsheep(Wangetal.,2017)
• NumberofmasAAscasesindairycows(Rodriges-MoQaetal.,2007)
FiPng of ZIP distribu*ons
The interpreta*ons of gene*c parameters are not trivial under ZIP models • ThereisnoobviouslyusefuldefiniAonofheritability(Rodrigues-MoQaetal.,2007).
• WeassessedtheimportanceofaddiAvegeneAcdifferencesbetweenanimalsfijngmodelswithoutandwithaddiAvegeneAceffect.
• WeassessedtherelaAonshipbetweenphenotypesandbreedingvalues(underliabilityscale).
Low phenotypic correla*on between parasite counts
0.11(0.09,0.13)
0.04(0.02,0.06)
0.14(0.11,0.16)
Gene*c varia*on was found to be important
Addi:vegene:ceffectParasite Without WithTick 42,532 42,515Nematodes 38,545 38,527Eimeriaspp. 33,366 33,359
DevianceinformaAoncriterionaccordingtostaAsAcalmodels
Gene*c varia*on was found to be important
Tick Nematode Eimeriaspp.Effect Mean Median S.D. Mean Median S.D. Mean Median S.D.
Animal(A) 0.140 0.137 0.023 0.289 0.289 0.012 0.187 0.176 0.047Permanentenvironment(Pe)
0.211 0.209 0.018 0.238 0.238 0.009 0.600 0.596 0.046
Group(Gr) 1.037 1.030 0.085 0.416 0.412 0.038 4.658 4.615 0.046
A/(A+Pe+Gr) 0.101 0.306 0.034(A+Pe)/(A+Pe+Gr) 0.253 0.559 0.145
Correla*ons between breeding values are low to moderate
0.30(0.27,0.32)
0.01(-0.02,0.03)
-0.07(-0.09,-0.04)
Moderate rela*onships between breeding values and phenotypes
0.59(0.56,0.62)
0.77(0.75,0.79)
0.41(0.37,0.45)
Tick Nematode Eimeriaspp.
Animals with the best breeding values have smaller parasite counts
Animals with the best breeding values have smaller parasite counts
Animals with the best breeding values have smaller parasite counts
Conclusions
• ThereisgeneAcresistanceofNellorecaQletobothAcksandgastro-intesAnalparasitesAcks.
• TickandnematodescountsmaybegeneAcallycorrelated.
• BreedingvaluesunderliabilityscalesareusefulforselecAonofresistantanimals.
Next steps
• GWASforresistancetoAcksandgastro-intesAnalparasites.
• TofitmodelsaccounAngforinfecAvity.
• IstheregeneAccorrelaAonbetweenresistanceandinfecAvity?
Acknowledge
• UFMG• CNPq• FAPEMIG• CAPES• MundoNovoRanch
• RonaldoLeite• EduardoCardoso• DanielGoncalves• AndreaWilson• OsvaldoAnacleto
Acknowledge
• JuanPabloBoteroCarrera• TiagoLucianoPassafaro• LiviaLoioladosSantos• DalinnedosSantos• FernandaRaidan• MullerGomes• GlausenLima
• AdrianeBarbosa• LarissaKretli• MarianaMamedes• AndresaMelo• GabrielaGouveia• VirgíniaRibeiro• LuizaAbreu• Etc…Thankyou!