Investigating biopesticides for the control of cabbage ... Project.pdf · biopesticides in the lab...
Transcript of Investigating biopesticides for the control of cabbage ... Project.pdf · biopesticides in the lab...
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Claire Hoarausupervised by
Tom Pope and Heather Campbell
Investigating biopesticides for the control of
cabbage stem flea beetle (Psylliodes chrysocephala) and the optimisation of conventional
synthetic insecticides
@CSFB_Hoa
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Context
Cabbage stem flea beetle (CSFB) Oilseed rape crop Severe economic damage from reduced yields or total crop failure
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Project aim
Imidacloprid
Clothianidin
Thiamethoxam
Pyrethroids
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Imidacloprid
Clothianidin
Thiamethoxam
Project aim
Pyrethroids
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Imidacloprid
Clothianidin
Thiamethoxam
Project aim
Pyrethroids
Oilseed rape growers need other solutions to control the
cabbage stem flea beetle!
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Azadirachtin (neem extract)
Nematodes
Bacillus thuringiensis, a bacterium
Project aimSteinernema spp.
Heterorhabditis spp.
CSFB infected with a fungus
Metarhizium spp.Beauveria spp.
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Tender loving care
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Tender loving care
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Experiments
Screening of biopesticides in the lab against adults
and larvae
Nematodes bioassays on adult stage >>
X 10
Steinernema feltiae
For each species, 3 repetitions of 3 concentrations: 4000, 10000 and 40000 injective juveniles/ml
Incubation at 25°C for 4 days; mortality assessed after 2, 3 and 4 days
X 10
Steinernema carpocapsae
X 10
Heterorhabditisbacteriophora
X 10
water
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Experiments
Screening of biopesticides in the lab against adults
and larvae
Nematodes bioassays on 3rd larval stage >>
1xFor each nematode species, 2 replicates. In each well: & 200 nematodes
Steinernema feltiae
Steinernema carpocapsae
Heterorhabditisbacteriophora
water
Incubation at 25°C for 40h. Mortality assessed at the end
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Experiments
Screening of biopesticides in the lab against adults
and larvae
Nematodes bioassays >>
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Experiments
Screening of biopesticides in the lab against adults
and larvae
Azatin bioassays on adult stage >>
X 15
0.5 ml/l
For each concentration, 3 repetitionsIncubation at 20°C for a week day; mortality assessed every day, leaf consumption assessed at the end
X 15
1 ml/l
X 15
1.4 ml/l
X 15
water
Solution of Azatin or water
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Experiments
Screening of biopesticides in the lab against adults
and larvae
Azatin bioassays on adult stage >>
At the end of the assay, consumption rate measured with Image J
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Experiments
Screening of biopesticides in the lab against adults
and larvae
Combination between
biopesticides and with pyrethroids
Investigation of diurnal patterns of CSFB susceptibility
to insecticides
Semi-field and field experiments
using selected insecticides
combinations
Study of the economical
feasibility of the successful
combinations