INTERNAL TECHNICAL ANNEX AGRIFOOD DEPARTMENT …©e-version... · Pesticide Residues Scope of...

Phytocontrol Analytics France, Parc Scientifique Georges Besse II – 180 rue Philippe Maupas – 30035 Nîmes - France Tél. +33 (0)4 34 14 70 00 - Fax. +33 (0)4 66 23 99 95 - www.phytocontrol.com - [email protected] SAS au capital de 1.000.000 euros - RCS Nîmes 490 024 049 - TVA intra FR 08 490 024 049 - APE 7120B

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Phytocontrol Laboratoire d’analyses

INTERNAL TECHNICAL ANNEX EA3.b-1 - Version 24 – 18/06/2018

INTERNAL TECHNICAL ANNEX AGRIFOOD DEPARTMENT

PHYTOCONTROL ANALYTICS France

Version 24 – June 12, 2018

References : Technical annex Cofrac No 1-1904 rev. 8 Technical annex Cofrac No 1-6066 rev. 6

LABORATOIRE PHYTOCONTROL (1)

Parc Scientifique Georges Besse II

180, rue Philippe Maupas

30035 NIMES,

under the accreditation number No 1-1904

LABORATOIRE PHYTOCONTROL (2)

Parc Scientifique Georges Besse

70 allée Graham Bell

30035 NIMES,

under the accreditation number No 1-6066

BIOTECHNOLOGICAL UNIT (Phytocontrol 1) ANALYTICAL CHEMISTRY UNIT (Phytocontrol 1, Phytocontrol 2) MICROBIOLOGY UNIT (Phytocontrol 2)


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Pesticide Residues Scope of accreditation No 1-1904

Flexible scope (FLEX3)

General scope*

Food and food products/ Foodstuffs / Physical-chemical analyses

Analysis of pesticide residues and organic contaminants in foodstuffs intended for human or animal consumption, biological matrices of animal origin - LAB GTA 26/99-2

OBJET CARACTERISTIQUE MESUREE OU RECHERCHEE PRINCIPE DE LA METHODE

Products of plant origin Products of animal origin Animal feed

Pesticide Residues

Extraction: Solid/Liquid Purification (if necessary) : Liquid-Solid (SPE) Liquid-Solid (SPE dispersive) Analysis : LC/MS-MS, GC/MS-MS, GC-MS

* FLEX3 Flexible Scope: The laboratory is recognised as competent, in the area covered by the general scope, to adopt any recognised method and to develop or implement any other method for which it has provided validation.


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Detailed scope



ITEM CHARACTERISTIC MEASURED OR SOUGHT PRINCIPLE OF THE METHOD REFERENCE OF

THE METHOD

Products of plant and animal origin: Watery product, High oil content products, Acid and water-rich products, Products rich in sugar and low in water, Products low in water and fat, Spices, aromatic and medicinal plants, Alcoholic beverages,

Fruit and vegetable juices

Diquat/Paraquat

Preparation/Extraction : Solid / liquid cold extraction Purification : SPE Analysis : LC-MS/MS

In-house method MOC3/20

Non-fat products of vegetable origin :

Watery product

Products rich in starch and protein Acid products Highly-pigmented products

Determination of the content of Chlormequat and Mepiquat

Extraction: by solvent Analysis : LC-MS-MS

In-house method

MOC3/21

Products of plant origin:

Watery product Products rich in starch and protein Acid products Highly-pigmented products

Determination of residues of Dithiocarbamates

Preparation/Extraction : Hydrolysis Analysis : Determination of residual CS2 by GC-MS

In-house method

MOC3/01


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THE METHOD

Products of plant origin: Watery product Products rich in starch and protein Acid products Highly-pigmented products

Determination of pesticides multiresidues Organophosphates: Chlorpyriphos ethyl Isofenphos methyl, Malathion, Parathion methyl, Phosalone, Pirimiphos methyl, Tolclophos methyl, Chlorfenvinphos, chlorpyriphos-methyl, dichlorfenthion, ethoprofos, fenchlorfos, fonofos Organochlorines : Chlorpropham, Procymidone, Propyzamide, Vinchlozoline, Myclobutanil, Triadimefon, Triadimenol, 2-4'DDE, 2-4'DDD, 4-4'DDE, 4-4''DDT, chlorobenzylate, fenarimol, fenhexamide, hcb, hch alpha, hch beta, hch delta, mirex oxadiazon, pentachloroanisole, tebufenpyrad Pyrethroids: Bifenthrin, Cyhalothrin Nitrogenous / miscellaneous : Bromopropylate, Cyprodinil, Diphenylamine, Pirimicarb, Propyconazole, Pyrimethanil, Fludioxonil, O-phenylphenol, Oxadixyl, Benalaxyl, bitertanol, carfentrazone-ethyl, chorthal-dimethyl, cyproconazole, dichlofop-methyl, difenoconazole, flusilasole, mepanipyrim, mepronil, penconazole, perthane, proquinazid, pyriproxyfen, tebuconazole Polychlorinated biphenyls (PCBs) : PCB 28, PCB 52, PCB 101, PCB 118, PCB 138, PCB 153, PCB 180.

Extraction : Solid/liquid cold extraction

Purification : SPE

Analysis : GC-MS GC/MS-MS

In-house method

MOC3/25


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THE METHOD

Products of plant origin: High oil content products

Products of animal origin : Dairy products Meat products Fats Fish products Egg products

Multi-residue pesticide dosing Organophosphates: Chlorfenvinphos, Chlorpyriphos ethyl, Chlorpyrifos methyl, Coumaphos Fenitrothion, Malathion, Methidathion Parathion methyl, Parathion ethyl, Phosalone, Pirimiphos methyl Ethion, Isofenphos methyl Pyridafenthion, Tolclophos methyl Organochlorates: Aclonifen, Chlorpropham, 2,4-DDD 2,4-DDE, 4,4’-DDE, 4,4’ DDT, Dieldrin Endosulfan alpha, Endosulfan beta Endosulfan sulphate, HCB, Oxyfluorfen Procymidone, Propyzamide Vinchlozoline, Myclobutanil Carfentrazone ethyl, Cyproconazole Diclofop methyl, Difenoconazole Fenarimol, Penconazole, Tebuconazole Tebufenpyrad Pyrethroids: Bifenthrine, Cyfluthrine, Cyhalothrine Cypermethrine, Deltamethrine Fluvalinate, Tefluthrine, Tetramethrine Organonitrogen groups / other: Bromopropylate, Propyconazole Fludioxonil, Benalaxyl, Cyprodinil Diflufenican, Flusilasole, Mepronil Metalaxyl, Pirimicarb, Proquinazid, Prosulfocarb, Pyriproxifen

Extraction : Solid/liquid cold extraction Purification : SPE dispersive Analysis : GC-MS/MS

In-house method

MOC3/26


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THE METHOD

Products of plant origin: Watery product Products rich in starch and protein Acid products Highly-pigmented products Products low in water and fat

Ethephon


Analysis: LC-MS-MS

In-house method

MOC3/27

Products of plant origin: Watery product, Acid and water-rich products, Products low in water and fat Products rich in sugars and low in water Alcoholic beverages, Fruit and vegetable juices, Infant nutrition

Determination of the content of fentin (expressed as triphenyltin cation), fenbutatin oxide, cyhexatin and azocyclotin.

Extraction : by solvent Purification : Liquid/solid (SPE dispersive) Analysis : LC-MS/MS

In-house method

MOC3/31

Products of plant origin: Watery product Products rich in starch and protein Acid products

Determination of the content of Maleic Hydrazide

Extraction : Solid/liquid cold extraction Analysis : LC-MS/MS

In-house method

MOC3/44

Products of plant origin: Watery product, Acid and water-rich products, Products rich in sugar and low in water, Products low in water and fat, Alcoholic beverages, Fruit and vegetable juices, Infant nutrition

Determination of the content of ethylene thiourea (ETU) and propylene thiourea (PTU)

Extraction : Solid/liquid cold extraction Purification : Liquid/liquid Analysis : LC-MS-MS



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THE METHOD

Products of plant origin: Watery product, Acid and water-rich products, Products low in water and fat Products rich in sugars and low in water Alcoholic beverages, Fruit and vegetable juices, Sodas

Determination of the content of Acetochlore, Alachlore, Benfluraline, Clomazone, Diflufenican, Ethofumesate, Etofenprox, Fenpropathrine, Fenvalerate, Fluopicolide, Hexazinone, Metolachlore, Permethrine, Pyridaben, Tefluthrine, Terbufos, Terbuthylazine, Triallate, Zoxamide.

Extraction : by solvent Purification : Liquid/solid (SPE dispersive) Analysis : GC-MS/MS


Products of plant origin: Infant nutrition

Multi-residue pesticide dosing Terbufos, Fipronil, Fipronil desulfinyl, HCB, Haloxyfop 2ethylhexyl, Haloxyfop methyl, Terbufos sulfone, Heptachlor, Heptachlor epoxide cis, Heptachlor epoxide trans Endrin, Disulfoton, Dieldrin, Aldrin, Demeton S Methyl, Nitrofen

Extraction : Solid/liquid cold extraction Purification : Liquide/Solide (SPE) Analysis : GC-MS/MS

In-house method

MOC3/56


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THE METHOD


Infant nutrition

Multi-residue pesticide dosing Haloxyfop (free acid), Terbufos sulfoxide, Ethoprophos, Fensulfothion, Fensulfothion oxon, Fensulfothion oxon sulfone, Fensulfothion sulfone, Disulfoton sulfone, Disulfoton sulfoxide, Cadusafos


Analysis : LC-MS/MS

In-house method

MOC3/57

Non-fat products of plant origin : Watery product, Acid and water-rich products, Alcoholic beverages, High oil content products (oil seeds) Products low in water and fat Products Other : teas Animal feed : Fodder, oilcake Mixed feeds

Determination of the content of Glyphosate and AMPA



Products of plant origin: Watery product Acid and water-rich products Alcoholic beverages Fruit and vegetable juices

Determination of the content of Fosetyl-Aluminum and Phosphoric Acid


In-house method

MOC3/89


Watery product

Acid and water-rich products

Sugar-rich and water-poor products

Alcoholic beverages

Fruit and vegetable juices

Determination of the content of Chlorate and Perchlorate


In-house method

MOC3/120


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THE METHOD


Watery product Acid and water-rich products

Multiresidue pesticide dosing Phenoxyacetic herbicides : 2.4.5 T, 2.4 D, 2.4 DB, DNOC, MCPA

Phenoxypropionic herbicides : Dichloprop P, Diclofop acid Fluazifop (free acid), Haloxyfop P Mecoprop P, Quizalofop Ureas : Amidosulfuron, Diflubenzuron Hexaflumuron, Teflubenzuron Thifensulfuron methyl, Triflumuron Nitriles : Bromoxynil, Ioxynil, Dinitrophenols : Dinoseb, dinoterb Pyridines : Triclopyr, Fluroxypyr Other : Bentazone, Dicamba, Dithianon MCPB, Orizalin


In-house method

MOC3/90


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THE METHOD

Products of plant origin: Watery product Acid and water-rich products Products rich in sugar and low in water Fruit and vegetable juices Sodas Alcoholic beverages Low water and fat products

Multi-residue pesticide dosing Benzimidazoles : Thiabendazole Ureas : Chlorotoluron, Diuron, Lufenuron Novaluron, Pencycuron Triazoles : Metconazole, Prothioconazole desthio, Etoxazole Triazines : Simazine, Terbumeton Carbamates : Benthiavalicarb isopropyl, Methomyl Oxamyl, Propoxur Organophosphores : Dimethoate, Omethoate Other : 1-naphtylacetamide, Ametoctradin Bifenazate, Carbetamide Carboxin, Chlorantraniliprole, Chloridazon, Clofentezine, Clothianidin, Cymoxanil, Dodine, Emamectin benzoate B1a, Etoxazole, Imidachlopride, Kresoxim-methyl, Mandipropamide Metamitron, Spinosad A + D Spiromesifen, Spirotetramate, Spirotetramate enol, Spirotetramate enol glucoside, Spirotetramate keto hydroxy, Spirotetramate mono hydroxy TCMTB, Thiachlopride Thiametoxam, Tricyclazole


In-house method

MOC3/97


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THE METHOD

Products of plant origin: Watery product Products rich in starch and protein Acid products Highly-pigmented products

Multi-residue pesticide dosing Benzimidazoles : Carbendazime, Thiophanate-methyl Strobilurines : Azoxystrobine, trifloxystrobine, Fluoxastrobine, picoxystrobine, pyraclostrobine Ureas : Isoproturon, linuron, metoxuron, triflusulfuron-methyl, Bensulfuron-methyl, buturon, cycluron, flufenoxuron, fluometuron, methabenzthiazuron, monolinuron, monuron, neburon Triazoles : Epoxyconazole, fenbuconazole, fetraconazole, Azaconazole, bromuconazole, paclobutrazole, triticonazole, Triazines : Cyanazine Carbamates : Iprovalicarb, thiodicarb

Pyridilmethyamines : Acetamipride Other :

Lenacil, metoxyfenoside, phenmedipham, rotenone, tebufenozide, Boscalid, butafenacil, cloquintecet, cyazofamide, desmedipham, desmetryn, dimethanamide, dimethomorphe, fenamidone, fenpyroximate, flurtamone, hexythiazox, indoxacarbe, isoporthiolane, isoxathion, metrafenone, phoxim, picolinafen, propaquizafop, pyraflufen-ethyl, spirodiclofen, spiroxamine, triflumizole, warfarine

Preparation/Extraction : Solid/liquid cold extraction

Analysis : LC-MS/MS

In-house method

MOC3/35


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ITEM CHARACTERISTIC MEASURED OR SOUGHT PRINCIPLE OF THE METHOD

REFERENCE OF THE METHOD

Beehive products :

Honey

Royal Jelly

Pollen

Bees

Multi-residue pesticide dosing : 2,4 DDD, 2,4 DDE, 4,4 DDE, 4,4 DDT, Alachlor, Bromopropylate, Chlordane (cis+trans), Chlorobenzilate, Chlorpyriphos ethyl, Chlorpyriphos methyl, Cyhalothrine, Cymiazole, Cypermethrine, Deltamethrine, Dichlobenil, Dieldrin, Difenoconazole, Endosulfan alpha, Endosulfan beta, Endrin, Ethion, Fenitrothion, Tau-fluvalinate, HCH alpha, HCH beta, Malathion, Metolachlor, Oxadiazon, Oxyfluorfen, Parathion methyl, Permethrine, Pirimiphos methyl, Procymidone, Profenofos, Prothiofos, Quinalfos, Tebufenpyrad, Tetradifon, Trifluraline, Vinchlozoline.

Extraction : Solid/liquid cold extraction Purification : SPE dispersive Analysis : GC-MS/MS

In-house method

MOC3/76

Products of animal origin : Meat products Egg products and by-products

Fipronil, Fipronil sulfone

Preparation / Extraction : Solid/liquid cold extraction Purification : SPE Analysis : GC-MS/MS


Products of animal origin : Meat products Egg products and by-products.

Amitraz (including metabolites containing the 2,4-dimethylaniline moiety expressed as amitraz)

Preparation / Extraction : Hydrolysis Solid/liquid cold extraction Purification : SPE dispersive Analysis : LC-MS/MS



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Products of plant origin: Watery product Acid and water-rich products Sugar-rich and water-poor products Products low in water and fat Alcoholic beverages Fruit and Vegetable Juices Sodas

Multi-residue pesticide dosing : 6-Benzyladenine, Acephate, Acetamipride, Ametoctradine, Amidosulfuron, Azaconazole, Azimsulfuron, Azinphos-ethyl, Azinphos-methyl, Azoxystrobine, Beflubutamide, Bensulfuronmethyl, Benthiavalicarbisopropyl, Bixafen, Boscalide, Bromacil, Bromuconazole, Bupirimate, Buprofezin, Buturon, Cadusafos, Carbendazim, Carbetamide, Carboxine, Chlorantraniliprole, Chloridazon, Chlorotoluron, Chloroxuron, Chlorsulfuron, Chromafenozide, Cinidonethyl, Cinosulfuron, Clethodim-sulfoxide, Clofentezine, Clothianidine, Cyanazine, Cyantraniliprole, Cyazofamide, Cycluron,Cyflufenamid, Cymoxanil, Cyprosulfamide, Demeton-S, Demeton-S-methylsulfone, Demeton-S-methylsulfoxide, Desmetryn, Difenamide, Diflubenzuron, Dimethenamid- P, Dimethoate, Dimethomorphe, Dinoseb, Dinoterb, Disulfoton-sulfone, Disulfoton-sulfoxide, Diuron,DMST, Dodemorphe, Dodine, Emamectine-benzoate B1a, Emamectine-benzoate B1b, Epoxiconazole, Ethametsulfuron-methyl, Ethidimuron, Ethiprole, Ethirimol, Etoxazole, Fenamidone, Fenamiphos sulfone, Fenamiphossulfoxide, Fenbuconazole, Fenchlorphos oxon, Fenoxaprop-ethyl, Fenoxycarbe, Fenpropidine, Fenpyramazine, Fenpyroximate, Fensulfothion, Fensulfothionoxon, Fensulfothion-oxonsulfone, Fensulfothionsulfone, Fenthion, Fenthion sulfone, Fenthion sulfoxide, Fenuron, Florasulam, Fluazinam, Flufenoxuron, Fluometuron, Fluopyram, Fluoxastrobine, Flupyradifurone, Flupyrsulfuron methyl, Fluquinconazole, Flurtamone, Fluxapyroxad, Foramsulfuron, Forchlorfenuron, Fosthiazate, Fuberidazole, Furametpyr, Halauxifen methyl, Halfenprox, Halosulfuronmethyl, Hexythiazox, Hydramethylnon, Imazalil, Imazamox, Imazaquin, Imazosulfuron, Imidachlopride, Indoxacarb, Iodosulfuronmethyl, Ioxynil, Iprovalicarbe, Isazofos, Isocarbophos, Isoprocarb, Isoprothiolane, Isoproturon, Isopyrazam, Isoxaben, Isoxaflutole, Isoxathion, Kresoxim-methyl, Lenacil, Linuron, Lufenurone, Mandipropamide, MCPA, Mecarbam, Mesosulfuronmethyl, Metaflumizone, Metamitron, Metconazole

Preparation/ Extraction: Solid/liquid cold extraction Purification : SPE Analysis : LC-MS/MS



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Products of plant origin: Watery product Acid and water-rich products Sugar-rich and water-poor products Products low in water and fat Alcoholic beverages Fruit and Vegetable Juices Sodas

Methabenzthiazuron, Methomyl, Methoxyfenozide, Metobromuron, Metolcarb, Metosulam, Metoxuron, Metrafenone, Metsulfuronmethyl, Mevinphos, Monalide, Monocrotophos, Monolinuron, Monuron, NAD(1-naphtyl acetamide), Napropamide, Neburon, Nicosulfuron, Norflurazon, Novaluron, Ofurace, Omethoate, Orthosulfamuron, Oxamyl, Oxasulfuron, Paclobutrazol, Paraoxon-ethyl, Pencycuron, Penflufen, Penoxsulame, Penthiopyrad, Phenmediphame, Phorate sulfone, Phorate-oxon, Phosphamidon, Phoxim, Picolinafen, Picoxystrobine, Pinoxadene, Pirimicarbdesmethyl, Promecarb, Prometon, Propamocarbe, Propaphos, Propaquizafop, Propoxur, Prothioconazoledesthio, Pyraclofos, Pyraclostrobine, Pyraflufenethyl, Pyrimidifen, Pyriofenone, Pyroquilon, Pyroxsulam, Rimsulfuron, Rotenone, Sedaxane, Silthiofam, Simazine, Spinetoram A, Spinetoram B, Spinosad A, Spinosad D, Spirodiclofen, Spiromesifen, Spirotetramate, Spirotetramate-enol, Spirotetramate-enolglucoside, Spirotetramate-keto-hydroxy, Spirotetramate-monohydroxy, Spiroxamine, Sulfosulfuron, TCMTB, Tebufenozide, Tebutam, Tebuthiuron, Teflubenzuron, Tepraloxydim, Terbumeton, Terbumeton desethyl, Tetraconazole, Thiabendazole, Thiachlopride, thiamethoxam, Thiencarbazone methyl, Thifensulfuron-methyl,Thiobencarb, Thiodicarb, Thionazin, Thiophanatemethyl, Tricyclazole, Trifloxystrobine, Triflumuron, Triflusulfuron-methyl, Triticonazole, Tritosulfuron, Vamidothion, Warfarin

Preparation/ Extraction: Solid/liquid cold extraction Purification : SPE Analysis : LC-MS/MS



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Scope FLEX3

General scope*



OBJET CARACTERISTIQUE MESUREE OU RECHERCHEE PRINCIPE DE LA METHODE


Organic Contaminant Residues

Extraction : by solvent Purification : Liquid-Solid (SPE) Analysis : UFLC, LC-MS/MS, GC-MS/MS


Detailed scope




THE METHOD

Products of plant origin: Watery product, Acid and water-rich products, Alcoholic beverages, Products rich in sugar and low in water, Products low in water and fat, Fruit and vegetable juices, Sodas Products of animal origin : Dairy products Meat products Fish products

Determination of the content of DDAC and BAC (QAC Analysis)

Preparation/ Extraction : Solid/liquid cold extraction

Analysis : LC-MS-MS

In-house method

MOC3/145


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FIXED Scope




THE METHOD

Products of plant origin: Watery product (Water content ≥ 60%) High oil content products Acid and water-rich products Products rich in sugar and low in water Other products Alcoholic beverages Fruit and vegetable juices Sodas

Products of animal origin : Dairy products

Determination of nitrate, nitrite, chloride, bromide content

Preparation / Extraction : Water Analysis : HPLC/CI (conductivity measurement)


Products of plant origin: High oil content products, Products low in water and fat (cerals and by-products, Fruits and vegetables powder) Fruit and vegetable juices, Alcoholic beverages Products rich in sugar and low in water Animal feed : Flour of animal origin Mixed feeds Raw materials of vegetable origin Products of animal origin : Dairy products including Infant nutrition Egg products Meat products Fish products

Melamine

Extraction :

Solvant

Analysis :

LC-MS/MS


FIXED Scope : The laboratory is recognized as competent to perform the tests in strict compliance with the methods mentioned in the scope of accreditation. Technical changes to the operating mode are not permitted.


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Heavy Metals Scope of accreditation N°1-1904

Scope FLEX3

General scope*

# Food and food products/ Foodstuffs / Physical-chemical analyses

Analysis of metallic and mineral trace elements and their chemical species in foodstuffs intended for human or animal consumption - LAB GTA 45


Foodstuffs for humans and animals (including infant nutrition)

Metals Minerals

Mineralisation Wet process (microwave digestion in closed system) Wet process (acid digestion in open system) Analysis : ICP/MS LC-ICP/MS



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Detailed scope

# Food and food products/ Foodstuffs / Physical-chemical analyses

Analysis of metallic and mineral trace elements and their chemical species in foodstuffs intended for human or animal consumption - LAB GTA 45


THE METHOD

Foodstuffs for humans and animals (including infant nutrition)

Arsenic, Lead, Cadmium, Mercury, Antimony, Barium, Boron, Chromium, Cobalt, Copper, Tin, Manganese, Molybdenum, Nickel, Palladium, Platinium, Iridium, Lithium, Rhodium, Ruthenium, Thallium, Vanadium

Mineralisation : Wet process (microwave digestion in closed system)

Wet process (open system digestion) Analysis : ICP-MS

In-house method

MOC3/85

Dairy products including infant nutrition Aluminium

Mineralisation : Wet process (microwave digestion in closed system)

Wet process (acid digestion in open system)

Analysis : ICP-MS

In-house method

MOC3/85

Alcoholic beverages Iron

Mineralisation : Wet process (acid digestion in open system)

Analysis : ICP-MS

In-house method

MOC3/85

Cereals Fruits and vegetables Fruit and vegetable juices Medicinal plants Beehive products Fish products Dairy products including infant nutrition

Arsenic III, Arsenic V, monomethyl Arsenic, dimethyl Arsenic, Arsenocholine AsC, Arsenobetaine AsB


Analysis : LC-ICP/MS


Fish products Fruits and vegetables Mushrooms Medicinal plants Food supplements Animal feed

Mercury II HgII, Methylmercury MeHg


Analysis : LC/ICP-MS



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Human food : Grain products, Fatty products, Egg products, Dairy products, Meat products, Fish products, Fruits and vegetables, Sweet products, Non-alcoholic beverages, Alcoholic beverages, Spices and condiments, Aromatic and medicinal plants, Special and dietary foods, Mixed feeds, Infant nutrition Animal feed : Raw materials, Complete or complementary compound feedingstuffs

Calcium, Magnesium, Phosphorus, Potassium

Mineralisation : Wet process (open system digestion) Analysis : ICP-MS

In-house method

MOC3/152


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Mycotoxins Scope of accreditation N°1-1904

Scope FLEX3

General scope*


Determination of mycotoxins and phycotoxins in food and feed - LAB GTA 21/99-1


Plant and animal products Raw material, derived and/or processed products

Determination of mycotoxins

Extraction : by solvent Purification : SPE Immunoaffinity Analysis : UFLC/LC-MS/MS

* Flexible scope FLEX3 : The laboratory is recognized as competent, in the field covered by the General scope, to adopt any recognized method and to develop or implement any other method including it will have ensured validation.


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Detailed scope




THE METHOD

Cereals Oilseeds Nuts Dried fruit Legumes Cereal Derivatives Products derived from oilseeds and nuts Fruit products : Compotes, Juices Alcoholic beverages Spices Coffee/Cocoa Coffee/Cocoa Products Food and drink for children Animal feed

Determination of the content of Ochratoxine A

Extraction : by solvent Purification : Immunoaffinity Analysis : UFLC


Fresh fruit and fruit products including children's food (fruit-based baby food)

Determination of the content of patuline

Extraction/purification: Solvant/SPE

Analysis : LC-MS-MS


Cereals Oilseeds Nuts Dried fruit Legumes Cereal Derivatives Products derived from oilseeds and nuts Fruit products : Compotes (including Infant nutrients) Food for children Animal feed

Determination of the content of aflatoxines (B1, B2, G1, G2)

Extraction : by solvent

Purification : Immunoaffinity

Analysis : UFLC



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THE METHOD

Cereals Cereal by-products Fresh fruit Fruit products Infant nutrition Animal feed : Products derived from oilseeds : Oilseed cake

Determination of the content of : Deoxynivalenol (DON), Fumonisines (B1+B2, B3), HT2 toxine, T2 toxine, Zearalenone (ZEA), Aflatoxines (B1, B2, G1, G2), Ochratoxine A (OTA)

Extraction / purification: Solvant / SPE


Analysis : UFLC


Spices Dry plants Coffee and cocoa and their by-products

Determination of the content of aflatoxines (G2, G1, B2, B1) et en ochratoxine



Analysis : LC-MS-MS


Milk and all dairy products including infant food Dairy products containing cereals

Determination of the content of Aflatoxine M1



Analysis : UFLC


Cereals Determination of the content of Deoxynivalenol (DON)



Analysis : UFLC


Cereals, Cereal by-products Legumes (pulses) Fresh vegetables, Leafy vegetables, Animal feed, Oilcake

Determination of the content of Datura Alkaloids

Extraction : By solvent Purification : SPE Analysis : LC-MS-MS


Cereals Cereal by-products Legumes (pulses) Fresh vegetables

Determination of the content of Ergot alkaloids

Extraction : by solvent Purification : SPE Analysis : UFLC

In-house method

MOC3/122

Cereals Determination of the content of Zearalenone (ZEA)

Extraction : by solvent Purification : SPE Analysis : UFLC

In-house method

MOC3/60


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Alkaloids Scope of accreditation N°1-1904

Scope FLEX3

General scope*






Extraction : by solvent Purification : Liquide-Solide (SPE) Analysis : UFLC, LC-MS/MS, GC-MS/MS


Detailed scope




THE METHOD

Spices Piperine

Preparation/Extraction : Solid/liquid cold extraction Purification : SPE Analysis : LC-MS/MS


Potato Chaconine and solanine Preparation/Extraction : Solid/liquid cold extraction Analysis : LC-MS/MS



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General scope*




Plant and animal products Raw material, derived and/or processed products

Determination of mycotoxins

Extraction : by solvent Purification : Immunoaffinity SPE Analysis : UFLC/LC-MS/MS


Detailed scope




THE METHOD

Products of plant and animal origin: Leafy vegetables Cereals and cereal by-products Aromatic and medicinal plants Food supplements Spices Honey

Echimidine, Echimidine-N-oxide, (Z)-Erucifoline, (Z)-Erucifoline-N-oxide, Europine hydrochloride, Europine-N-oxide, Heliotrine, Heliotrine-N-oxide, Indicine hydrochloride, Indicine-N-oxide, Integerrimine, Integerrimine-N-oxide, Jacobine, Jacobine-N-oxide, Lasiocarpine, Lasiocarpine-N-oxide, Lycopsamine, Lycopsamine-N-oxide, Monocrotaline, Monocrotaline-N-oxide, Retrorsine, Retrorsine-N-oxide, Senecionine, Senecionine-N-oxide, Senkirkine, Seneciphylline, Seneciphylline-N-oxide, Senecivernine, Senecivernine-N-oxide, Trichodesmine, Intermedine, Intermedine-N-oxide.

Preparation/Extraction : By solvent Purification : SPE Analysis : LC-MS/MS



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GMOs Scope of accreditation N°1-1904

Scope FLEX3

General scope*

Agri-food / Vegetables / Molecular Genetics

Analysis related to genetically modified organisms - GMOs


Corn Crude and/or processed products

PCR target plant species PCR target of a GMO sequence :

- screening sequence - specific event sequence

Homogenisation / Grinding Extraction Real-time PCR (qualitative and quantitative)

Soy Crude and/or processed products

PCR target plant species PCR target of a GMO sequence : - screening sequence - specific event sequence

Homogenisation / Grinding Extraction Real-time PCR (qualitative and quantitative)



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Detailed scope


Analysis related to genetically modified organisms - GMOs

OBJET CARACTERISTIQUE

MESUREE OU RECHERCHEE

DOMAINE D’APPLICATION

ETENDUE DE MESURE

PRINCIPE DE LA METHODE

REFERENCE DE LA METHODE

Plant species Maize

Target plant species-specific PCR: ADH

Raw products (seeds, grains, flour, etc.) and processed products

Qualitative/quantitative

Homogenisation/ Grinding Extraction PCR in real time

In-house method adapted from standards

NF EN ISO 21569, 24276, 21570 and 21571 and

their respective amendments on MON 810, GA21, NK 603 and

MON 863 maize

MOC3/103

Plant species Maize

Target plant sequence-specific PCR P35S screening





NF EN ISO 21569, 24276, 21570 and 21571 and


MON 863 maize

MOC3/103

Plant species Maize

Target GMO sequence-specific PCR Tnos screening





NF EN ISO 21569, 24276, 21570 and 21571 and


MON 863 maize

MOC3/103


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ETENDUE DE MESURE



Plant species Maize

Target GMO sequence-specific PCR Identification of specific event MON810


Qualitative


Internal method adapted to the NF EN

ISO 21569,24276,21570 and 21571 standards and their respective

amendments on MON 810 maize.

MOC3/103

Plant species Maize





Internal method adapted to the NF EN

ISO 21569,24276,21570 and 21571 standards and their respective

amendments on MON 810 maize.

MOC3/103

Plant species Maize

Target GMO sequence-specific PCR Identification of specific event NK603




Internal method adapted to NF EN ISO 21569,24276,21570 and 21571 standards and their respective

amendments on NK603 maize.

MOC3/103

Plant species Maize

Target GMO sequence-specific PCR Identification of specific event GA21


Qualitative



amendments on GA21 maize.

MOC3/103

Plant species Maize

Target GMO sequence-specific PCR Identification of specific event Bt11


Qualitative


Internal method adapted from NF EN

ISO 21569,24276,21570 and 21571 and their

respective amendments on Bt11

maize.

MOC3/103


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ETENDUE DE MESURE



Plant species Maize

Target GMO sequence-specific PCR Identification of specific event Mon88017




Internal method adapted to NF EN ISO

21569,24276,21570 and 21571 standards and their respective amendments on

maize Mon88017

MOC3/103

Plant species Maize

Target GMO sequence-specific PCR Identification of specific event T25


Qualitative


Internal method adapted to the standards... Validation

according to NF EN ISO 21569,24276,21570 and

21571 standards and their respective amendments on

maize T25

MOC3/103

Plant species Maize

Target GMO sequence-specific PCR Identification of specific event TC1507


Qualitative


Internal method adapted to the standards... Validation

according to NF EN ISO 21569,24276,21570 and

21571 standards and their respective amendments on

maize TC1507

MOC3/103

Plant species Soya

Target plant species-specific PCR: ADH: Lectine




In-house method adapted to NF EN ISO


RRS soybeans.

MOC3/103

Plant species Soya

Target GMO sequence-specific PCR P35S screening






RRS soybeans.

MOC3/103

Plant species Soya

Target GMO sequence-specific PCR TNOS screening






RRS2 soybeans.

MOC3/103


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ETENDUE DE MESURE



Plant species Soya

Target GMO sequence-specific PCR Identification of specific event RRS





21569,24276,21570 and 21571 standards and their respective amendments

on FG72 soybeans.

MOC3/103

Plant species Soya

Target GMO sequence-specific PCR Identification of specific event RRS2






on RRS2 soybeans.

MOC3/103

Plant species Soya

Target GMO sequence-specific PCR Identification of specific event FG72






on FG72 soybeans.

MOC3/103

Plant species Soya



Qualitative



21569,24276,21570 and 21571 standards and their respective amendments on Mon87701 soybeans.

MOC3/103

Plant species Soya

Target GMO sequence-specific PCR Identification of specific event A2704-12


Qualitative



21569,24276,21570 and 21571 standards and their respective amendments on A2704-12 soybeans.

MOC3/103

Plant species Soya

Target GMO sequence-specific PCR Identification of DAS-81419


Qualitative

Homogenization/ grinding

Manual and automated extraction

Real-time PCR



MOC3/103


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ETENDUE DE

MESURE



Plant species Soya

Target GMO sequence-specific PCR Identification of MON87751


Qualitative

Homogenization/ grinding Manual and automated extraction Real-time PCR


amendments MOC3/103

Plant species Soya

Target GMO sequence-specific PCR Identification of DAS-68416-4


Qualitative



amendments MOC3/103

Plant species Soya

Target GMO sequence-specific PCR Identification of DAS-44406-6


Qualitative



amendments MOC3/103


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Allergènes Scope of accreditation N°1-1904

Scope FLEX3

General scope*



Grain products Fruits and vegetables Sweet products Tea and infusion

Detection of specific target DNA of an allergenic protein Detection in simplex

Grinding /Homogenisation Manual extraction of DNA by silica gel column adsorption Amplification by real time PCR (qualitative method)



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Detailed scope


ITEM CHARACTERISTIC MEASURED OR SOUGHT PRINCIPLE OF THE METHOD REFERENCE OF THE METHOD

Cereal products: Raw material: soybeans, barley Processed products: (granules, cake, cookies) Sweet and sweetened products: (processed products): chocolate, rice and chocolate desserts, jam, cake mixtures, cake. Fruits and vegetables: Tapioca, soya milk, coconut, milk

Specific target DNA sequence: Cashew nuts: Ana o (1.010 vicilin-like protein) Almond: prunin 1 precursor Hazelnut: Cor a 1 Nuts: 2S albumin seed storage protein precursor


In-house method MOC3/115: Grinding /Homogenisation C3/01-01. D Extraction of ADN: NucleoSpin®Plant II (Macherey-Nagel)

Real-time PCR: according to MOC3/115 ‘annex 1’

Target DNA sequence specific: Pistachio: COR gene dehydrin Pecan nuts: pec2a1a (7S vicilin)


In-house method MOC3/115: Grinding /Homogenisation IC3/01-01.D Extraction of ADN: NucleoSpin®Plant II (Macherey-Nagel) Real-time PCR: according to MOC3/115 ‘annex 2’

Cereal products: Raw material: soybeans, barley Processed products: (granules, cake, cookies) Tea and infusion: Tea, Jasmine flower Sweet and sweetened products: cake mixtures, cake

Target DNA sequence specific for peanut: Arah 1 gene


In-house method MOC3/115:

Grinding /Homogenisation IC3/01-01.D Extraction of ADN: NucleoSpin®Plant II

(Macherey-Nagel) Real-time PCR: according

to: according to MOC3/115

‘annex 1’


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Cereal products: Raw material: soybeans, barley Processed products: (granules, cake, cookies) Sweet and sweetened products: cake mixtures, cake

Specific target DNA sequence for sesame: 2S albumin


In-house method MOC3/115: Grinding /Homogenisation C3/01-01. D Extraction of ADN: NucleoSpin®Plant II (Macherey-Nagel) Real-time PCR: according to MOC3/115 ‘annex 1’

Cereal products: Raw material: corn flour, lupin flour, tapioca starch, chestnut flour Processed products: (granules, fondant, pancakes, muffins, cookies)

Specific target DNA sequence for Brazilian nuts: 2S albumin (ber e1)



Cereal products: Raw material: corn flour, lupin flour, tapioca starch, chestnut flour. Processed products: (granules, fondant, pancakes, muffins, cookies)

Sweet and sweetened products: Soy yogurt, soy cream, fruit compote, vegetable drinks

Target DNA sequence specific soya: Lectine


In-house method MOC3/115: Grinding /Homogenisation C3/01-01. D Extraction of ADN: NucleoSpin®Plant II

(Macherey-Nagel) Real-time PCR: according to

MOC3/115 ‘annex 1’


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Cereal products: Raw material: corn flour, lupin flour, tapioca starch, chestnut flour. Processed products: fondant, filled pancakes, filled muffins, chocolate chip cookies, prepared baby food dishes, frozen patties, zucchini, vegetable terrine, verrine meat.

Sweet and sweetened products: Soy yogurt, soy cream, vegetable drinks

Target DNA sequence specific celery: 5.8 ribosomal RNA



Cereal products: Raw material: corn flour, lupin flour, tapioca Processed products: fondant, pancakes, muffins Sweet and sweetened products: Turron, soy fruit cream, fruit compote, vegetable drinks

Target DNA sequence specific lupine: conglutin alpha mRNA




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Scope FLEX3

General scope*

Food and food products/ Allergens / Immunology


Cereal products

Alcoholic and non-alcoholic drinks

Compound feed

Meat production

Fish products

Sweet and sweetened products

Dairy products

Detection and quantification of allergenic proteins

Grinding /Homogenisation Extraction of proteins ELISA



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Detailed scope

Food and food products/ Allergens / Immunology

ITEM CHARACTERISTIC MEASURED OR SOUGHT PRINCIPLE OF THE METHOD REFERENCE OF THE

METHOD

Cereal products

Compound products

Raw cereals and primary products: wheat, rye, barley, barley, oats, spelt and their hybrid strains, rice, sorghum, quinoa, lentils, chick peas, flour and derivatives, cereal flakes, tapioca, buckwheat, brewer's yeast, guar gum.

Mix for pastry Pizzas Spreads Cereal puree and vegetables, ravioli Breakfast cereals Sauce preparations Spices and aromatic plants

Detection and quantification of gluten


In-house method MOC3/119 according to supplier kit: R7001 RIDASCREEN® Gliadin (R. BIOPHARM)

Fish products Fresh and Canned Fish

Alcoholic beverages

Wine

Detection of histamine Grinding /Homogenisation Extraction of proteins ELISA

In-house method MOC3/135 version 1 according to supplier kit: R1601 RIDASCREEN® Histamine (R. BIOPHARM)


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ITEM CHARACTERISTIC MEASURED OR SOUGHT PRINCIPLE OF THE METHOD REFERENCE OF THE

METHOD

Non-alcoholic beverages

Cereal products

Sweet products

Compound products

Almond milk; Soy milk; Fruit juice Raw cereals and primary products Blown cereals Cereal products containing chocolate Sorbets & water ice cream Prepared meals based on cereals and vegetables Babyfood based on cereals and vegetables

Detection and quantification of casein


In-house method MOC3/125 version 2 according to supplier R4612 RIDASCREEN®FAST Casein (R. BIOPHARM)

Cereal products

Compound products

Meat products

Dairy products

Beverages

Raw cereals and primary products Blown cereals Babyfood Chile Bolognese sauce Soup Cheese Pork ham and pork chorizo wine

Detection and quantification of egg protein


In-house method MOC3/125 according to supplier kit: R6402 RIDASCREEN®FAST Ei /Egg Protein (R. BIOPHARM)

Almond milk

Soy milk

Crude cereals and products of

primary processing - Blown

cereals

Chile

Bolognese sauce

Wine

Detection and quantification of total milk protein


In-house method MOC3/125 according to supplier kit R4652 RIDASCREEN®FAST Milk (R. BIOPHARM


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Grain products (raw and first processing) : Corn flour, soya, quinoa, wheat semolina Grain products processed: Mini plum, Mix dry pastry, Cookies, sandwich bread, Mixed feeds: Chili con carne, Bolognese sauce, Mediterranean pizza, Doy passato BBF ; Sweet products: Organic candy, Hazelnut ice cream, Glucose syrup, Chocolate

Detection and quantification of beta-lactoglubin


In-house method MOC3/190 selon kit fournisseur R4912 RIDASCREEN® FAST β-Lactoglobulin (R. BIOPHARM)


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Contaminants from packaging and materials Scope of accreditation N°1-1904

Scope FLEX3

General scope*

Food processing / Other foods / Physical and chemical analyses







Detailed scope




THE METHOD

Products of plant origin: Watery product Acid and water-rich products Products rich in sugar and low in water Products low in water and fat Alcoholic beverages Fruit and vegetable juices Sodas Products of animal origin : Dairy products including infant nutrition

Bisphenol A

Extraction : Solid/liquid cold extraction Purification : SPE Analysis : LC-MS/MS



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REFERENCE OF THE METHOD

Products of plant origin: Alcoholic beverages, Oils

Determination of the content of Phthalates and other plasticisers: - DMP (Dimethyl phtalate) -DiBP (Di-iso-butyl phtalate) -DBP (Di-n-butyl phtalate) -BBP (Benzylbutyl phtalate) -DiPP (Di-iso-pentyl phtalate) -nPiPP (n-pentyl-iso-pentyl phthalate) -DPP (Di-n-pentyl phtalate) -DHxP (Di-n-hexyl phthalate) -DEHP (Bis(2-ethylhexyl) phtalate) -DCHP (Dicyclohexyl phtalate) -DiHpP (Di-iso-heptyl phtalate) -DnOP (Di-n-octyl phtalate) -DEHT (Bis(2-ethylhexyl) terephtalate) -DiNP (Di-iso-nonyl phtalate) -DNP (Di-n-nonyl phtalate) -DiDP (Di-iso-decyl phtalate) -DiBA (Di-iso-butyl adipate) -DBA (Di-n-butyl adipate) -DINCH (1,2-cyclohexanedicarboxylic acid, diisononyl ester) -Tributyl O-acetylcitrate - DMEP (Bis(2-methoxyethyl) phtalate) - DMiP (Dimethyl isophtalate) - DMT (Dimethyl terephthalate) - DPhP (Diphenyl phthalate) - DAP (Diallyl phthalate) - DEP (Diethyl phthalate) - TBP (tributylphosphate) - DEA (Diethyl adipate) - DEHA (Bis(2-ethylhexyl) adipate) - DVA (Divinyl adipate)

Extraction : Liquid/liquid cold extraction Analysis : GC-MS-MS



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Neoformed contaminants : Scope of accreditation N°1-1904

Scope FLEX3

General scope*





Pesticide Residues

Extraction: Solid/Liquid Purification (if necessary) : Liquid-Solid (SPE) Liquid-Solid (SPE dispersive) Analysis : LC/MS-MS, GC/MS-MS, GC-MS



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Detailed scope




THE METHOD

Products of plant and animal origin: Tea, cocoa, vegetable oils, soy sauce, hydrolyzed vegetable proteins, infant milk

3-MCPD (free) 2-MCPD (free) Glycidol (free)

Preparation/Extraction: Solid / cold liquid Liquid / cold liquid Purification: Derivation Analysis : GC-MS/MS



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Scope FLEX3

General scope*









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Detailed scope




THE METHOD

Products of vegetal origin : Spices Aromatic and medicinal plants Products rich in sugar and low in water Watery product, High oil content products, Acid and water-rich products, Products low in water and fat, Alcoholic beverages, Fruit and vegetable juices, Sodas Products of animal origin : Beehive products, Dairy products, Meat products, Fish products, Fats Animal feed : Animal feed Other : Cacao

Polycyclic aromatic hydrocarbons : Benzo(a)anthracène, Benzo(b)fluoranthène, Benzo(a)pyrène, Chrysène.

Preparation / Extraction : Liquid/liquid cold extraction Purification : SPE Analysis : GC-MS/MS


Products of vegetal origin : High oil content products Cereals and by-products Cacao Products of animal origin : Dairy products Meat products Fats Fish products Infant nutrition

Polycyclic aromatic hydrocarbons: Benzo(a)anthracène, Benzo(b)fluoranthène, Benzo(a)pyrène, Benzo(k)fluoranthène, Chrysène, Dibenzo(a, e)pyrene, Dibenzo(a, l)pyrene, Fluoranthène, 5-methylchrysene, Pyrène

Extraction : Solid/liquid cold extraction Purification : SPE Analysis : UFLC



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Products of plant origin: Cereals and by-products Cacao Tea and coffee High oil content products Infant nutrition Products of animal origin : Dairy products Meat products Fats Fish products Infant nutrition Animal feed : Raw materials of vegetable origin

Polycyclic aromatic hydrocarbons: Acenaphtène, Acenaphtylène, Benzo(g,h,i)perylène, Benzo(k)fluoranthène, Benzo(j)fluoranthène, Cyclopenta(c,d)pyrene, Dibenzo(a,h)anthracène, Dibenzo(a, l)pyrene, Fluorène, Indeno(1,2,3-cd)pyrène, 5- methylchrysene. Anthracene Phenanthrene Fluoranthene Pyrene

Extraction : Solid/liquid cold extraction Purification : SPE Analysis : GC-MS/MS



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THE METHOD

Products of plant origin: Watery product and by-products, Cereals and by-products, Products rich in sugar and low in water, Nuts, Vegetable and Fruit juices, Wine, cider, beer, coffee, tea Products of animal origin : Meat products Fish products Milk, Yogurt

Determination of the content of Acrylamide

Extraction : Solid/liquid cold extraction Purification : SPE Analysis : LC-MS/MS



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Dioxins and PCBs Scope of accreditation N°1-1904

FIXED Scope




THE METHOD

Products of plant origin: High oil content products Watery product Acid and water-rich products Products rich in sugar and low in water Products low in water and fat Infant nutrition Other products: spices, coffee, tea, aromatic and medicinal plants Products of animal origin : Dairy products (cheese) Egg products Meat products Fish products Infant nutrition Animal feed : Flours of animal origin Mixed feeds Raw materials of vegetable origin Mineral Compounds

Polychlorodibenzo-p-dioxines (PCDD) : 2,3,7,8-TCDD, 1,2,3,7,8-PeCDD, 1,2,3,4,7,8-HxCDD, 1,2,3,6,7,8-HxCDD, 1,2,3,7,8,9-HxCDD, 1,2,3,4,6,7,8-HpCDD, OCDD

Polychlorodibenzofurannes (PCDF) : 2,3,7,8–TCDF, 1,2,3,7,8-PeCDF, 2,3,4,7,8-PeCDF, 1,2,3,4,7,8-HxCDF, 1,2,3,6,7,8-HxCDF, 1,2,3,7,8,9-HxCDF, 2,3,4,6,7,8-HxCDF, 1,2,3,4,6,7,8-HpCDF, 1,2,3,4,7,8,9-HpCDF, OCDF,

PCB “dioxins like” : PCB77, PCB81, PCB126, PCB169, PCB105, PCB114, PCB118, PCB123, PCB156, PCB157, PCB167, PCB189

PCB « Non dioxins Like » (indicators) : PCB28, PCB52, PCB101,

PCB138, PCB 153, PCB180

Extraction :

Under hot pressure

(PFE)

Purification:

SPE

Analysis :

GC-HRMS

Dilution isotopique



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THE METHOD

Products of plant origin: High oil content products (vegetable oils) Alcoholic beverages Fruit and vegetable juices Soda Products of animal origin : Dairy products (milk, yoghurt, very fatty products) Fats Infant nutrition Animal feed : Fats

Polychlorodibenzo-p-dioxines (PCDD) : 2,3,7,8-TCDD, 1,2,3,7,8-PeCDD, 1,2,3,4,7,8-HxCDD, 1,2,3,6,7,8-HxCDD, 1,2,3,7,8,9-HxCDD, 1,2,3,4,6,7,8-HpCDD, OCDD,

Polychlorodibenzofurannes (PCDF) : 2,3,7,8–TCDF, 1,2,3,7,8-PeCDF, 2,3,4,7,8-PeCDF, 1,2,3,4,7,8-HxCDF, 1,2,3,6,7,8-HxCDF, 1,2,3,7,8,9-HxCDF, 2,3,4,6,7,8-HxCDF, 1,2,3,4,6,7,8-HpCDF, 1,2,3,4,7,8,9-HpCDF, OCDF,

PCB “dioxines like” : PCB77, PCB81, PCB126, PCB169, PCB105, PCB114, PCB118, PCB123, PCB156, PCB157, PCB167, PCB189

PCB « Non dioxines Like » (indicators) : PCB28, PCB52, PCB101, PCB138, PCB 153, PCB180

Extraction :

Liquid-Liquide

Purification:

SPE

Analysis :

GC-HRMS

Isotopic dilution


FIXED Scope : The laboratory is recognized as competent to practice the methods described in strict compliance with the recognized methods mentioned in the Scope of accreditation.


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Scope FLEX3

General scope*






Preparation/Extraction : Solid / liquid cold by solvent Purification : Liquid-Solid (SPE) Analysis : UFLC-FLD, LC-MS/MS, GC-MS/MS


Detailed scope


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THE METHOD

Products of plant origin: High oil content products Infant nutrition Products of animal origin : Dairy products (cheese) Egg products Meat products Fish products Infant nutrition Animal feed : Farines d’origine animales Mixed feeds Minerals compound

Polychlorodibenzo-p-dioxines (PCDD) : 2,3,7,8-TCDD, 1,2,3,7,8-PeCDD, 1,2,3,4,7,8-HxCDD, 1,2,3,6,7,8-HxCDD, 1,2,3,7,8,9-HxCDD, 1,2,3,4,6,7,8-HpCDD, OCDD Polychlorodibenzofurannes (PCDF) : 2,3,7,8–TCDF, 1,2,3,7,8-PeCDF, 2,3,4,7,8-PeCDF, 1,2,3,4,7,8-HxCDF, 1,2,3,6,7,8-HxCDF, 1,2,3,7,8,9-HxCDF, 2,3,4,6,7,8-HxCDF, 1,2,3,4,6,7,8-HpCDF, 1,2,3,4,7,8,9- HpCDF, OCDF PCB “dioxins like” : PCB77, PCB81, PCB126, PCB169, PCB105, PCB114, PCB118, PCB123, PCB156, PCB157, PCB167, PCB189 PCB “non dioxins like” (indicators) : PCB28, PCB52, PCB101, PCB138, PCB 153, PCB180

Preparation / Extraction : By solvent Purification: SPE Analysis : GC-MS/MS Isotopic dilution

In-house method

MOC3/180


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Products of plant origin: High oil content products (vegetable oils) Products of animal origin : Dairy products Fats Infant nutrition Animal feed : Fats

Polychlorodibenzo-p-dioxines (PCDD) : 2,3,7,8-TCDD, 1,2,3,7,8-PeCDD, 1,2,3,4,7,8-HxCDD, 1,2,3,6,7,8-HxCDD, 1,2,3,7,8,9-HxCDD, 1,2,3,4,6,7,8-HpCDD, OCDD Polychlorodibenzofurannes (PCDF) : 2,3,7,8–TCDF, 1,2,3,7,8-PeCDF, 2,3,4,7,8-PeCDF, 1,2,3,4,7,8-HxCDF, 1,2,3,6,7,8-HxCDF, 1,2,3,7,8,9-HxCDF, 2,3,4,6,7,8-HxCDF, 1,2,3,4,6,7,8-HpCDF, 1,2,3,4,7,8,9- HpCDF, OCDF PCB “dioxins like” : PCB77, PCB81, PCB126, PCB169, PCB105, PCB114, PCB118, PCB123, PCB156, PCB157, PCB167, PCB189 PCB “non dioxins like” (indicators): PCB28, PCB52, PCB101, PCB138, PCB 153, PCB180

Preparation / Extraction : By solvent Purification: SPE Analysis : GC-MS/MS Isotopic dilution

In-house method

MOC3/181


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Veterinary drug residues Scope of accreditation N°1-1904

Scope FLEX3

General scope*

# Food processing / Other foods / Physical and chemical analyses

Analysis of authorised or unauthorised substances for veterinary or zootechnical use (veterinary medicinal products) - LAB GTA 30/99-6


Biological matrices of animal origin Chloramphenicol

Preparation : Extraction by solvent Purification : SPE dispersive Analysis : LC-MS/MS


Detailed scope

# Food processing / Other foods / Physical and chemical analyses

Analysis of authorised or unauthorised substances for veterinary or zootechnical use (veterinary medicinal products) - LAB GTA 30/99-6


THE METHOD

Œufs Muscles Milk Honey Fish products :fish, shellfish, crustaceans

Chloramphenicol

Preparation : Extraction by solvent Purification : SPE dispersive Analysis : LC-MS/MS



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Food colouring Scope of accreditation N°1-1904

FIXED Scope

Agri-food / Other foods, Beverages (excluding drinking water) and Sweet products / Physico-chemical analysis

Physico-chemical analyses for the determination of composition, quality and technological criteria, and nutritional labelling in human food and animal nutrition - LAB GTA 25/60-118


THE METHOD

Spices and condiments Mixed feeds Sauce

Dosing of dyes: Auramine, Fast garnet GBC, Oil orange SS, Para red, P-nitroaniline, Sudan blue 2, Sudan I, Sudan II, Sudan III, Sudan IV, Sudan red 7B, Sudan red G, Sudan yellow, Toluidine red, Vert de leucomalachite.

Extraction : by solvent Analysis : LC-MS/MS



Dosing of dyes: E101, E110, E122, E123, E124, E129, E131, E132, E133, E151

Extraction : by solvent Analysis : UFLC-DAD




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Nutritional values Scope of accreditation N°1-1904

Scope FLEX3

General scope*

Agri-food / Other foods, Dairy products, Animal feed, Fats, Beverages (excluding drinking water) and Sweet products, Grain products / Analyses physico-chemical

Physico-chemical analyses for the determination of composition, quality and technological criteria, and nutritional labelling in human food and animal feed - LAB GTA 25/60-61-81-82-118-119


Human food Animal feed Determination of the content of sodium

Preparation : Mineralisation (wet process) Analysis : ICP-MS



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Detailed scope

Agri-food / Other foods, Dairy products, Animal feed, Fats, Beverages (excluding drinking water) and Sweet products, Grain products / Analyses physico-chemical



THE METHOD

Human food : Fruits and vegetables Mixed feeds Dairy products Fatty products Sweet products Grain products Egg products Meat products Fish products Coffee, Teas, Infusion Non-alcoholic beverages Spices and condiments Dietetic foods, diet foods, special foods Animal feed : Complete or complementary mixed feeds Raw material for anaimal nutrition

Determination of the content of total sodium and calculation of salt content

Preparation : Mineralisation (wet process) Analysis : ICP-MS



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Scope FLEX3

General scope*

Agri-food / Other aliments, Dairy products, Animal feed, Fats, Beverages (excluding drinking water) and Sweet products, Grain products / Analyses physico-chemicals



Human food Determination of carbohydrate components

Preparation : Water extraction Analysis : Ion chromatography / Pulsed amperometry


Detailed scope




THE METHOD

Human food : Fruits and vegetables Mixed feeds Dairy products Sweet products Grain products Dietary foods

Determination of the content of Fructose, Glucose, Lactose, Maltose, Saccharose

Preparation : Water extraction Analysis : Ion Chromatography / Pulsed Amperometry



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Nutritional values Scope of accreditation N°1-1904

FIXED Scope


Physico-chemical analyses for the determination of composition, quality and technological criteria, and nutritional labelling in human food and animal nutrition - LAB GTA 25/82-118


THE METHOD

Fatty products Sweet products Fat extraction for characterisation

Extraction by solvent : n-hexane / Isopropanol 3 /2 (v/v)


Fatty products Sweet products

Multi-residue determination of methyl esters, fatty acids

Preparation : Methylation Analysis : GC-FID




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FIXED Scope


Physical-chemical analyses for the determination of composition, quality and technological criteria, and nutrition labelling in food and feed - GAB GTA 25/60-61-80-81-82-118-119


THE METHOD

Human food : Fruits and vegetables Dietary foods Diet foods Special power supply Mixed feeds Spices and condiments Dairy products Meat products Fish products Sweet products Coffee, tea, infusion Grain products Animal feed : Mixed feeds

Determination of water activity

Hygrometry (Principle of dew point)




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Scope FLEX3

General scope*


Physical-chemical analyses for the determination of composition, quality and technological criteria, and nutrition labelling in food and feed - LAB GTA 25/60-118-119


Human food

Determination of content of food fiber

Preparation/Extraction: Enzymatic digestion Analysis: Gravimetry


Detailed scope


Physical-chemical analyses for the determination of composition, quality and technological criteria, and nutrition labelling in food and feed - LAB GTA 25/60-118-119


THE METHOD

Fruits and vegetables Mixed feeds Sweet products Grain products

Determination of total dietary fibre

Preparation/Extraction: Enzymatic digestion Analysis: Gravimetry



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Scope FLEX3

General scope*




Human food Animal feed Determination of total fat content

Preparation : Hydrolysis Extraction by solvent Analysis: Gravimetry


Detailed scope




THE METHOD

Human food : Fruits and vegetables Mixed feeds Dairy products Fatty products without seeds Oilseeds Sweet products Grain products except Raw cereals Dietary foods Spices and condiments Meat products Non-alcoholic beverages Animal feed : Complete or complementary compound feedingstuffs

Determination of total fat content

Preparation : Hydrolysis Extraction by solvent Analysis: Gravimetry



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Scope FLEX3

General scope*




Human food Animal feed Determination of total nitrogen content

Kjeldahl : Mineralisation Distillation Titrimetry


Detailed scope




THE METHOD

Human food : Fruits and vegetables Mixed feeds Dairy products Fatty products Sweet products Grain products Dietary foods Spices and condiments Animal feed : Complete or complementary compound feedingstuffs

Determination of total nitrogen content and protein content calculation

Kjeldahl : Mineralisation Distillation Titrimetry



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Scope FLEX3

General scope*




Human food Animal feed

Determination of humidity Drying process Gravimetry

Determination of ash content Dry Mineralization Gravimetry



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Detailed scope




THE METHOD

Foodstuff : Fruit and vegetables Compound foods Dairy products Non-seeded fat products oleaginous Sweet and sweetened products Cereal products except crude cereals Dietetic foods Spices and condiments Animal feed: Complete or complementary compound feedingstuffs

Determination of dry matter content or moisture content

Drying process Gravimetry


Determination of ash content Dry Mineralization Gravimetry



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Scope FLEX3

General scope*

Agri-food / Other foods, Beverages (excluding drinking water) and Sweet products / Physico-chemical analyses

Physico-chemical analyses for the determination of composition, quality and technological criteria, and nutritional labelling in human food - LAB GTA 25/60-118


Human food

Determination of sulphite content

Monier-Williams optimized method: Distillation Titrimetry


Detailed scope

Agri-food / Other foods, Beverages (excluding drinking water) and Sweet products / Physico-chemical analyses

Physico-chemical analyses for the determination of composition, quality and technological criteria, and nutritional labelling in human food - LAB GTA 25/60-118


THE METHOD

Foodstuff :

Fruit and vegetables

Compound foods


Dried fruit, dried tomatoes, mustard

Determination of sulphite content

Monier-Williams optimized method: Distillation Titrimetry



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Food Microbiology Scope of accreditation N°1-6066

Scope FLEX1

Agri-food / Other foods / Microbiological analyses

Microbiological analysis of food products and environment - LAB GTA 59


THE METHOD

Products intended for human consumption, feed and environmental samples

Micro-organisms Colony counts at 30°C by deep seeding technique NF EN ISO 4833-

1

Products intended for human consumption, feed and environmental samples

Micro-organisms Colony counts at 30°C using surface seeding technique NF EN ISO 4833-

2

Products intended for human consumption or animal feed, environmental samples in the agri-food sector

Entero-bacteriaceae

Colony counts at 37°C (or 30°C)

NF ISO 21528-2

All food and feed products and samples of the production plant working environment

Entero-bacteriaceae Colony counts at 37°C

BRD 07/24-11/13


Coliforms

Colony counts at 30°C (or 37°C)

NF ISO 4832

Products intended for human consumption or animal nutrition Thermotolerant coliforms

Colony counts at 44°C NF V08-060

Products intended for human consumption or animal nutrition

Escherichia coli - β-glucuronidase positive

Colony counts at 44°C NF ISO 16649-2

All human food products

Coliforms

Colony counts at 37°C by chromogenic medium RAPID E.coli 2

BRD 07/08-12/04

All food and feed products Escherichia coli

-β-glucuronidase positive

Colony counts at 37°C by chromogenic medium RAPID E. coli 2

BRD 07/07-12/04


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THE METHOD

All food and feed products Enterobacteriaceae Colony counts at 37°C by chromogenic medium REBECCA™ + EB

AES 10/07-01/08

All food and feed products Escherichia coli - β - glucuronidase positive

Colony counts at 37°C by chromogenic medium REBECCA™ BASE or REBECCA™+ EB

AES 10/06-01/08


Escherichia coli O157 Enrichment Separation / Concentration Isolation - Confirmation

NF EN ISO 16654


Positive coagulase staphylococcus Colony counts at 37°C by chromogenic agar medium Baird Parker

NF EN ISO 6888-1


Positive coagulase staphylococcus Aerobic colony counts at 35°C or 37°C using rabbit plasma and Fibrinogen agar media.

NF EN ISO 6888-2

Human food products and environmental sampling

Positive coagulase staphylococcus Colony counts at 37°C by specific media RAPID' Staph and confirmation

BRD 07/09-02/05


Sulfitore-reducing bacteria Anaerobic colony counts at 46°C in anaerobic conditions

NF V08-061


Anaerobic sulfitore-reducing bacteria

Colony counts at 37°C

NF ISO 15213


Clostridium perfringens Colony counts at 37°C and confirmation

NF EN ISO 7937


Presumptive Bacillus cereus Colony counts at 30°C

NF EN ISO 7932


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THE METHOD

All food and feed products Presumptive Bacillus cereus Enumeration at 30°C using Compass® Bacillus cereus Agar chromogenic medium

BKR 23/06-02/10


Mesophilic lactic acid bacteria Colony counts at 30°C NF ISO 15214

Meat and meat products Pseudomonas spp Colony counts at 25°C NF EN ISO 13720 Products intended for human consumption or animal nutrition

Yeasts and moulds Colony counts at 25°C NF V08-059


Yeasts and moulds developing on a medium with low water activity

Colony counts at 25°C NF V08-036


Listeria monocytogenes Colony counts at 37°C and confirmation

NF EN ISO 11290-2

All feeding products and environmental samples

Listeria monocytogenes et

Listeria spp

Enumeration at 37°C by chromogenic medium ALOA COUNT™

AES 10/05-09/06

Milk powders, infant milk powder Cronobacter spp Search Isolation / Identification and Confirmation

NF EN ISO 6579-1


Salmonella spp. of which Salmonella Typhi and Salmonella

Paratyphi

Search Isolation / Identification and Confirmation BRD 07/11-12/05

All food and feed products and samples from the production environment

Salmonella Search by Hromogenic Environment RAPID Salmonella

NF EN ISO 11290-1

All food and feed products Listeria monocytogenes Search Isolation / Identification and Confirmation

AES 10/03-09/00

Flexible Scope FLEX1: The laboratory is accredited to perform the tests according to the method described in the referential referred to in the version in force at the time of the evaluation. # Accreditation made compulsory under the French regulatory framework specified by the text cited in the document. Cofrac LAB INF 99 available at www.cofrac.fr.

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