InstructionsforUse ToolsforMALDI Imaging · BrukerDaltonics Table1...
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Revision 1 (July 2012) Instructions for Use Tools for MALDI Imaging MTP Slide Adapter II, Glass Slides and Cover Slips CARE products are designed to support our worldwide customers with high-quality consumables, accessories and dedicated kits. The CARE product range is specifically optimized and certified for use with all Bruker Daltonics systems. www.care-bdal.de / www.care-bdal.com Language: en
Transcript of InstructionsforUse ToolsforMALDI Imaging · BrukerDaltonics Table1...
Revision 1 (July 2012)
Instructions for UseTools for MALDI Imaging
MTP Slide Adapter II, Glass Slides and Cover Slips
CAREproducts are designed to support our worldwide customerswith high-qualityconsumables, accessories and dedicated kits.
The CARE product range is specifically optimized and certified for use with allBruker Daltonics systems.
www.care-bdal.de / www.care-bdal.com Language: en
Bruker Daltonics
1 Product Description 22 Software Requirements 3
2.1 Teaching and Spot Localization 43 Using Cover Slips 44 Mounting Glass Slides 55 Manufacturer 6
1 Product Description
The MTP Slide Adapter II (see Figure 1), dedicated glass slides and cover slips are tools for MALDIimaging. Two glass slides (75 x 25 x 0.9mm) can bemounted on theMTP Slide Adapter II.
Because MALDI targets must provide an electrically conductive surface, the glass slides have atransparent conductive ITO coating on one surface. Otherwise electrostatic charges will occur. Thesurface of the slides that is coated is indicated by the slide packaging (see Figure 2).
IMPORTANT Tissue samplesmust be applied to the surface of the slide with the ITO conductivecoating.
Cover slips (9 x 25 mm) are size-optimized and recommended for use in all ImagePrep samplepreparations to optimize the reproducibility of the sensor readout (see the ImagePrep User Manual formore details).
The products are for research use only. They are not for use in diagnostic procedures
Figure 1 MTP Slide Adapter II
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Bruker Daltonics
Figure 2 Glass slides for MALDI imaging
Ordering Information
Product Part number
MTP Slide Adapter II for MALDI Imaging # 235380
Glass Slides (75 x 25mm) for MALDI Imaging (100 pcs) # 237001
Cover slips for ImagePrep (200 pcs) # 267942
BigSlides (75 x 50mm, 100 pcs) for MALDI Imaging (require # 255595) # 259387
2 Software Requirements
Compass Software for flex Series 1.2. (flexControl 3.0) provides automated target geometryrecognition. Older software versions only recognize the MTP Slide Adapter II after a software updateand/or installation of a free software patch (see Table 1) . The software patch is available fromwww.bdal.com/Imaging or www.bdal.de/Imaging. Please close flexControl and flexAnalysis beforerunning the setup.
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Bruker Daltonics
Table 1 Requirements for automatic recognition of the MTP Slide Adapter II
Compass release 1.2 1.1 1.0
flexControl release 3.0 2.4 2.2 2.0 1.2 1.1
No software patchrequired
Software patchrequired
Software update required
Alternatively, theMTP 384 ground steel geometry in FlexControl can be loadedmanually inflexControl.
Note MALDI imaging applications require Bruker flexImaging software (#242166).
2.1 Teaching and Spot Localization
Bruker flexImaging software provides a convenient 3-point teaching procedure which matches themicroscopic image of the slide (or tissue section) to the actual target position in the instrument. Werecommend using either three slide corners or three distinct locations on the tissue section as teachingpoints.
Coarse navigation on tissue samples ismade very easy using the 384 array coordinates:
1. Load the slide(s) containing tissue samples into the slide adapter.
2. Place a transparent plasticMTP target lid (supplied with the adapter) onto the slide adapter,
3. Trace the position of the sample boundary and teachingmarks onto the plastic lid with amarkerpen.
4. Transfer the plastic lid to a standard MTP target to determine the coordinates of the sampleboundary and teaching point .
3 Using Cover Slips
Cover slips are used to optimize thematrix deposition procedure using the ImagePrep device.
Beforematrix deposition a cover slip is placed over a tissue-free area of the slide. The area of the slidewith the cover slip is then placed over the ImagePrep sensor window.
Figure 3 Slide with tissue and cover slip before ImagePrep matrix deposition
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Bruker Daltonics
4 Mounting Glass Slides
Note Glass slides aremounted from the rear of the slide adapter.
1. Place the slide adapter upside-down on a table or lab bench.
2. Make sure the beige colored retaining tabs are retracted into the body of the slide adapter.
3. Insert slides into the slide adapter with the tissue side facing downwards and lower gently intoplace.
The protruding screws at the front of the slide adapter prevent the tissue from contacting the tablesurface.
Figure 4 Inserting a slide into the slide adapter
4. Push the retaining tabs toward the center of the slide adapter to hold the glass slides in place.
Figure 5 Push the retaining clips toward the center to hold the slide in place
IMPORTANT The surface of the slide with the conductive coating and the tissue section must facethe front of the target.
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Bruker Daltonics
5 Manufacturer
Bruker Daltonik GmbHFahrenheitstraße 428359 BremenGermany
Support
E-mail: [email protected]: +49 (421) 2205-184Fax: +49 (421) 2205-104
Sales Information
E-mail: [email protected]: +49 (421) 2205-0Web: www.care-bdal.com
# 235380, 237001, 267942
Descriptions and specifications supersede all previous information and are subject to change without notice.
© Copyright 2012 Bruker Daltonik GmbH
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