Infection Control PBC PresTest
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Transcript of Infection Control PBC PresTest
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Peripheral Blood Culture Collection
Brandy Ballard BSN, RN; Jesika Sacomani RN; Norman Swope BSN, RN, OCN
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Gather supplies.Check procedure.Obtain butterfly
needle.Obtain Multi-vial
adapter.Obtain culture kit (comes
with tourniquet, chlorhexadine swab, alcohol swabs, gauze and a band aid.
Check culture bottles.
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Set up multi-vial adapter.
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Prep culture bottles.• Clean each bottle
with alcohol swab, and leave swab in place until ready to instill blood.
• Clean 15-20 seconds.
• The caps are not sterile.
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Skin Preparation
Scrub venipuncture site with chlorhexadine 30 seconds. Allow to dry, also 30 seconds.
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Do not re-palpate site after prep to avoid contamination.
BAD
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Venipuncture
One needle, One stick = Zero Contaminated Cultures
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Inoculation of cultures• Obtain 8-10
mL blood each bottle, Blue first.
• Label site, date, time initials accordingly.
KEYPOINT: If drawing with a winged butterfly set you must inject the blue topped Aerobic bottle first then the purple topped Anaerobic bottle. If drawing with a syringe, either directly or from a central line, then you must inject the purple topped Anaerobic tube first then the blue topped Aerobic tube last.This is opposite from CVL draws.
First Second
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Blood Culture Post ExamBrandy Ballard BSN, RN; Jesika Sacomani RN;
Norman Swope BSN, RN, OCN
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If multiple blood tubes and blood cultures are required from a peripheral stick which do you get first?A. blood tubes.B. the red blood culture bottle.C. the blue blood culture bottle.D. Makes no difference which you draw if
done using sterile technique.
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When drawing blood cultures from a central or arterial line which bottle do you inoculate first?
A. the pink bottle. B. the blue bottle.C. It does not matter.
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Before drawing blood cultures from a peripheral IV you must first change the infusion caps?A. TrueB. False
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How many ml’s of blood must be obtained for each culture bottle?A. 5 mlB. 20 mlC. 10 ml D. Just enough to tint the water.
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How long should you scrub the site before drawing your specimen for blood cultures?A. 30 secondsB. 60 seconds C. 5 minutes D. You do not need to clean the site before
drawing cultures!
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As you open the box that contains the culture bottles you accidently drop one. Do you use it?A. Sure! Why not!B. No, it is now considered damaged, and a
new culture set must be obtained. C. If the bottle looks ok then it is fine to use.
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To mix the blood in the bottle invert the bottle 4-5 times.A. TrueB. False
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Do you need to clean the septa of the culture bottle before inoculating?A. Yes, because the septa is not sterile. B. No, because the septa is sterile.
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How long should you wait before drawing the second set of blood cultures?A. 1 hourB. 12 hoursC. 5-30 mins.
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When obtaining blood cultures from an immune-suppressed patient do you waste any blood when drawing from a CVL?A. YesB. No
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Answer Key1. C 10. B2. A3. B 4.C5. A6. B7. A 8. A 9. C