In brain, one of the principal GSLs is sulfatide (SF), sulfated derivative of GalCer.
description
Transcript of In brain, one of the principal GSLs is sulfatide (SF), sulfated derivative of GalCer.
In brain, one of the principal GSLs is sulfatide (SF), sulfated derivative of GalCer.
Goals:
• To determine how SO3 group affects SF anchoring to GLTP
• To switch GLTP for SF selectivity
2-
Altered SF level is implicated in Alzheimer’s and
Parkinson’s diseases, multiple sclerosis, progressive
epilepsy with mental retardation and other
neurodegenerative human disorders
K55I
K55F
K55Y
K55Q
K55E
K55N
SF head group binding to GLTP
“OUT” “IN”
WT D48V
PART III. Structural Basis for Suppression of Human Trinucleotide Repeat Expansion Diseases (TREDs)
• repeated CNG-triplet sequences in genes• dynamically unstable, tend to expand• expanded tracts cause many muscle- and
neurodegenerative diseases
HYPOTHESIS
• Ye, K., Malinina, L., and Patel, D.J. (2003) Recognition of small interfering RNA by a viral suppressor of RNA silencing. Nature 426, 874-878
pGG(CAG)5CC• • • • • • •CC(GAC)5GGp
• • • • • • UC(GUC)5GUUp
pUUG(CUG)5CU
U•UU•U C•CC•CA•AA•A G•GG•G
12
3?
Sequence specific p19?
CNGCNGCNGCNGCNGCNGGNCGNCGNCGNCGNCGNC• • • • • • • • • • • • • • • • • •
2 3
Come to Bilbao
Research TeamBorja OchoaBorja Ochoa
Felipe GoñiFelipe Goñi
Sergey IvaninskiySergey Ivaninskiy Aintzane CaboAintzane Cabo
Elizaveta KatorchaElizaveta Katorcha
Sandra DelgadoSandra Delgado Valeria SamyginaValeria Samygina
Jevgenia TamjarJevgenia Tamjar