IMPLEMENTING A NETWORKING PLATFORM TO … · Presentation of the ALCUE Food project 3. A few...

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 1

Food Traceability: the key to global market access

Session 5: The European Program – New science in traceability

IMPLEMENTING A NETWORKING PLATFORM

TO PROMOTE AND ENHANCE SCIENCE AND TECHNICAL CO-OPERATION WITH EUROPE

IN FOOD QUALITY AND SAFETY


1st and 2nd of April 2008, Kuala Lumpur, Malaysia

Mathieu WEIL, Caroline BLACHE, Fabrice DAVRIEUX,

Didier MONTET, Marc LEBRUN, Dominique PALLET



IMPLEMENTING A NETWORKING PLATFORM

TO PROMOTE AND ENHANCE SCIENCE AND TECHNICAL CO-OPERATION WITH EUROPE

IN FOOD QUALITY AND SAFETY

CONTENTS

1. What is Cirad ?

2. Presentation of the ALCUE Food project

3. A few Cirad’s researches on traceability using innovative tools for Food Quality and Safety

- Polymerase Chain Reaction – Denaturant Gel Gradient Electrophoresis

- Electronic nose

- Near Infra Red Spectrometry



French Agricultural Research Centrefor International Development

workingworking for for developingdeveloping countries and the countries and the French French overseasoverseas regionsregions



Six strategic lines of researchEcological intensification

Biomass energy in favour of communities in the South

Safe, varied food

Animal health and emerging diseases

Public policy, poverty and inequality

Relations between agriculture, environment,nature and society



One operating principle: partnerships

Projects built and implemented hand-in-hand withresearch and development players• • ResearchResearch organizationsorganizations and and universitiesuniversities

• Professional • Professional organizationsorganizations and the and the privateprivate sectorsector

• Public • Public authoritiesauthorities and and donordonor agenciesagencies

• Local • Local authoritiesauthorities

• • NGOsNGOs

Working in the South



3 Departments - 60 research units1800 persons including 750 researchers

Metropolitan France: 430 researchers

Outside metropolitan France: 320 researchersFrench overseas regions = 110Other countries = 210, including 30 in international research centres

Cirad’s Organization



www.alcuefood.orgwww.alcuefood.org

“From European fork to Latin American farm”: an innovative networkingplatform for EU – LAC partnerships in food quality and safety R&D”

EC FP6 SSA Alcue-Food, 2005-08

2.



MAIN GOAL

To strenghthen the EU-LAC R&D cooperation through the effective involvement of LAC partners in european consortium on FQS

GENERAL OBJECTIVE

To implement a permanent EU-LAC platform on FQS

To develop and implement a

comprehensiveEU-LAC

information system on FQS

issues

SPECIFIC OBJECTIVES

To define EU-LAC stakes on FQS and state

priorities on elaboration of

joint R&D projects

To enhance the LAC capacity

building throughbetter use of the existing EU-LAC

cooperationinstruments

To propose to EC and/or europeanconsortia relevant

LAC "groups of excellence" for

further involvment in the new FP 6&7

instruments

Project’s objectives



KEY DATAS

Full project time : 36 months

Total human resources : 121 person months – 13 partners

4 EU Member States (France, Belgium, Spain, Portugal)4 Latin American MERCOSUR countries (Argentina, Brazil, Chile, Uruguay)

Total Budget : 737 500 euros

EXPECTED RESULTS

Strenghthening of trade in Food Sector

Compliance of LAC Food Exports with EU regulations

PROJECT CUSTOMERS

All stakeholders of the food supply chain : producers, firms, consumer associations, academic and research sector, policy makers, regulatory bodies

A totalfood chainapproach



TraceabilityTraceabilitywaswas identifiedidentified as one of major as one of major prioritypriority topicstopics

(existence of a knowledge, existence of research activities …)

Organization of Workshopsand

Training sessions

Elaborationof

joint projects

Data baseimplementation

Information System(Web portal)

Disseminationactivities

Projectactivities



Field: Traceability in the food chainField: Traceability in the food chain

Organization of workshops and training sessionsOrganization of workshops and training sessions

“Geo information in traceability systems, agricultural production systems and environmental management : opportunities in regional development”Campinas, Brazil, 27th of July 2005, 35 participants

“Recent advances on food traceability systems”Santiago, Chile, 1st of August 2005, 95 participants

“Opportunities for EU-LAC co-operation on food traceability systems”Santiago, Chile, 2-3 August 2005 (60 participants)

“Traceability systems along the meat chain”Buenos Aires, 4th of August 2005 (60 participants)

“Strategic Technology Watch - applied to traceability, why and how to use Internet efficiently”Buenos Aires, 28 November - 2 December 2005 (15 persons trained)



Offer interface with INCO (southern cone) countriesto FP6 project (SSA PETER)

Facilitate integration of INCO partners in the EC top-upprocedure (joining Integrated Projects or Network of Excellence)

Elaboration of new projects for the FP6/FP7(ex: SSA OTAG -Geodecision to Track and Trace Agricultural Production)

Elaboration of joint EUElaboration of joint EU--LAC ProjectsLAC Projects

Field: Traceability in the food chainField: Traceability in the food chain



Building a Database in traceability between the two regionsfor Key Stakeholders:

Producers, Entreprises and Traders

Scientists and Research Institutions

Consumer Groups and Policy Makers

Data base implementationData base implementation



Information System (www.alcuefood.org)Information System (www.alcuefood.org)



Through the Information System / Web Portal

Through Workshops and Trainings

Through Participation in PETER:- Disseminate European information to LAC/INCO countries- Put forward LAC/INCO demands/contexts for betteradequation of traceability systems to the real needs

Dissemination activitiesDissemination activities



developped in Joint Research Unit QualiSud

- PCR DGGE (fishes)

- Electronic nose (dates)

- Near Infra Red Spectrometry (coffee and cocoa)

3. A few Cirad’s researches on traceability usinginnovative tools for Food Quality and Safety



PangasiculturePangasiculture in Mekong delta (100.000 tons per year)in Mekong delta (100.000 tons per year)

Microbial ecology to trace originby Polymerase Chain Reaction – Denaturant Gel Gradient Electrophoresis

[email protected]



New method PCR-DGGE

Food sample

Total DNA includingmicrobial DNA and

Food DNA

Mixture of DNA amplicons from

species of microorganisms

Extraction of total DNA

PCR Amplification of a ribosomal DNA variable region by specific

primers

Comparative analysis of sequences and determination of microbial markers of specific area

Separation of differentDNA by DGGE



Dendrogramme de 25 Obs.Moy. non pond. grpes associés

Percent disagreement

0 20 40 60 80 100 120

(Dlien/Dmax)*100

AGB2P5-PAGB2P4-PAGB2P3-PAGB2P2-PAGB2P1-PAGB5P5-PAGB5P4-PAGB5P3-PAGB5P2-PAGB5P1-PAGB4P5-PAGB4P4-PAGB4P3-PAGB4P2-PAGB4P1-PAGB3P5-PAGB3P4-PAGB3P3-PAGB3P2-PAGB3P1-PAGB1P5-PAGB1P4-PAGB1P3-PAGB1P2-PAGB1P1-P

DGGE

PCR-DGGE

DGGE

UV transilluminator

Statistical analysis

Image analysis



DGGE prints of bacteria extracted from dry fishescoming from three different farms (rainy season)

Chau Phu An Phu Chau Doc

E.Coli

L. plantarum



CP1-RCP2-RCP3-RCP4-RCP5-R

CP1-DCP2-DCP3-DCP4-DCP5-D

AP1-RAP2-RAP3-RAP4-RAP5-RAP1-DAP2-DAP3-DAP4-DAP5-D

CD1-RCD2-RCD3-RCD4-RCD5-R

CD1 -DCD2 -DCD3 -DCD4 -DCD5 -DTC1-RTC2-RTC3-RTC4-RTC5-R

TC1-DTC2-DTC3-DTC4-DTC5-D

-12 -10 -8 -6 -4 -2 0 2 4 6 8 10

Dimen s ion 1 : 46.24% varia nces

-8

-6

-4

-2

0

2

4

6

8

Dim

ensi

on 2

: 26

.40%

var

ianc

es

CP: Chau Phu; CD: Chau Doc; TC: Tan Chau; AP: An PhuR: rainy season; D: dry season

Factorial analysis of DGGE fingerprint of bacteria from fishes of 4 districts in the province of Angiang (Vietnam) at rainy and dry seasons



Élec

tron

icH

uman

Electronic nose to distinguish dates [email protected]



Electronic noseMass spectrometer+ sensors

Visualization of the global finger prints



Distribution of Black Bousthammi, Deglet nour and their mix 50%on first principal plan



Near Infra Red Spectrometry

[email protected]



Near Infra Red Spectrometry: principle

Diatomic vibring Molecule

Infraredlight

(ν v, Ev)

(ν, λ) E = hν

The SPIR is the measurement of the absorption (Wave length and intensity) of the near infra-red light by a sample. The principle of the technic is based on the vibrational properties of the molecules and their interactions with the light.



ON

C H3

N

NNH3C

OC H

3

Spectral fingerprintComparaison to spectral data base

Infrared Light

Energy absorbance by Chemical Links Cafein

Qualitative AnalysisQuantitative Analysis



Characterization of coffee mixtures (roasted and grounded)

Robusta

Arabica

C P1 (63.86% )

C P2 (29.85% )

Distribution of coffee arabica and robusta on first principal plan

decaffeinated

Aromatized

with vanilla

defatted

Comparison of specific coffees to standard



Linear discriminant analysis of three origins of cocoa beans

-1

-0.8

-0.6

-0.4

-0.2

0

0.2

0.4

0.6

0.8

1

1.2

-1 -0.5 0 0.5 1 1.5 2

-- axe 1 (55% ) -->

-- ax

e 2

(45%

) -->

Côte d'Ivoire

TrinidadVenezuela

Classification rate 100%



ManyMany ThanksThanks for for youryour attention !attention !

www.cirad.fr/ur/qualite_aliments

www.cirad.fr

www.alcuefood.org

Mathieu WEIL [email protected]

IMPLEMENTING A NETWORKING PLATFORM TO … · Presentation of the ALCUE Food project 3. A few...

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