High Value Uses Of Waste Methane From Landfill And Biogas...
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www.sbrc-nottingham.ac.uk [email protected] Synthetic Biology Research Centre High Value Uses Of Waste Methane From Landfill And Biogas Methane From Anaerobic Digestion 22/01/2019 Alexander Grosse-Honebrink, Bashir Rumah, Christopher Stead, Ruth Cornock, Helen West, Andrew Goddard, Ying Zhang
Transcript of High Value Uses Of Waste Methane From Landfill And Biogas...
www.sbrc-nottingham.ac.uk [email protected]
Synthetic Biology Research Centre
High Value Uses Of Waste Methane From Landfill And Biogas Methane From
Anaerobic Digestion
22/01/2019
Alexander Grosse-Honebrink, Bashir Rumah, Christopher Stead, Ruth Cornock, Helen West, Andrew Goddard, Ying Zhang
In Confidence 1
Agriculture
UK “waste” Methane sources
Source: Grosse-Honebrink et al 2018, C1Net
In Confidence 2
Methane assimilation
Methane Methanol
Assimilation via
RuMP
(T I, X),
Serine Cycle
(T II)FormaldehydeFormateCarbon dioxide
O2 H2O
NADH NAD+
NADH NAD+
In Confidence 3
Possible products from methanotrophs
Solvents
Platform
chemicals
Single Cell Protein
Speciality
chemicals
EnzymesPHAMethanolFormaldehydeAcetic acidLactic acidSuccinic acidFarnesenePropylene
Lipids (biodiesel)EthanolButanol
Lipids (dietary supplements)
EctoineSurface LayersCarotinoidsSources: Grosse-Honebrink et al 2018, C1Net
Strong et al. 2015European Commission 2015
Sample production phase
Green natural production
Orange GM needed
In Confidence 4
Isolate new methanotrophic species capable of PHB production
Use waste methane streams to assess PHB production
Project description
In Confidence 5
Environmental Sampling
In Confidence 6
Isolation of methanotrophic bacteria
Neat
10-7
Neat
10-7
x3
2 weeks
• Rich Media
• Microscopy
• PCR
• Growth W/WO Methane
• NGS
In Confidence 7
Isolation of methanotrophic bacteria
Organism Methylomonas
Type I
Isolated from Droitwich water
In Confidence 8
Growth on biogas from AD
NH3 2.9 mg/m3H2S 94 ppm
NH3 <0.7 mg/m3H2S 463 ppm
In Confidence 9
PHB production on biogas
In Confidence 10
New method for PHB production
d0
d1
d2
d3
d4
d4 c
trl +N
O3-
0
1 0
2 0
3 0
4 0
5 0
P H B p ro d u c t io nP
HB
/dry
we
igh
t [%
]
Compared to 2% with old method
In Confidence 11
Landfill gas samples to test
Erin 1Erin 2
Parkwood 1Parkwood 2
Pilsworth
www.sbrc-nottingham.ac.uk [email protected]
Acknowledgements
University of NottinghamYing ZhangNigel P MintonHelen WestBashir RumahChristopher SteadRuth Cornock
Freeland Horticulture LtdAndrew Goddard
