H.Ghaderian1 1-Deparyment of Microbiology, Faculty of Biological Sciences, Islamic Azad Univercity,...
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Transcript of H.Ghaderian1 1-Deparyment of Microbiology, Faculty of Biological Sciences, Islamic Azad Univercity,...
H.Ghaderian1
1-Deparyment of Microbiology , Faculty of Biological Sciences , Islamic Azad Univercity , Falanarjan Branch , 804515/155 , Esfahan , Iran
: Classification
Family : Herpes viridea
Sub family : herpes virinea
Genus : simplex Specieas:HSV-1CL 101 ,HSV-2 strain KN 53690
common name : herpes symplex virus
tegument
Alpha herpes viruses
latently infected
Herpes virus in the host cell
HSV of the newborn is acquired 3 periods :
in utera
Peripartium (85%)
postpartum
Incidence of neonatal herpes
in 3 form :
1 )lesion of the skin , eyes , mouth
2 )Encephalitis with or without lesion
3 )Dissiminated desease in liver
lunges
gland adrenl
gut
Neonatal herpes
o case of neonatal herpes HSV -1and HSV-2
o Investigation showed Neonatal disseminated HSV infection is most frequently caused by HSV-2, although HSV-1 can also be the cause
o Serologic investigations showed no IgM and IgG antibodies for HSV-1 and HSV-2 in new born
Immune ResponsesNeonatal
Infected neonates produce HSV-specific IgM antibodies within 3 weeks of acquisition of the viral infection ,
which increase rapidly during the first 2 to 3 months
and may remain for as long as 1 year after neonatal infection develops.
Lymphocytes from infected infants
alpha-interferon
Neonates INF –αcomparewith adults with primary HSV infection is decreased.
The first time infection of the pregnant women may lead to severe illness in pregnancy and
may be virus transmission to newborn.
Study showed:
DIAGNISTIC
The high risk for death requires prompt diagnostic therfore suggestive that of HSV was identified by PCR
Viral DNA was extracted from all specimens(tracheal aspirate, liver, lungs, and gut)
By using the QIAamp DNA Mini Kit
1)PCR
primers for the HSV-1: thymidinekinase gene
((Fw 5′-AGCGTCTTGTCATTGGCGAA-3′
(Rev 5′-TTTTCTGCTCCAGGCGGACT-3′)
Primer for the HSV-2: DNA polymerase gene
((Fw 5′-CGTCCTGGAGTTTGACAGCG-3′
(Rev 5′-CAGCAGCGAGTCCTGCACACAA-3′)
The DNA was then used for HSV DNAPCR
Nucleotide sequence analysis
showed identical sequences in different specimens.
GenBank BLAST tool
highest similarity was
HSV-1 strain CL 101 and HSV-2 strain KN 53690
2)Cell culture
•Multi nucleated epithelial cells
treatment All infants with diagnosed HSV infectionmust be treated with an intravenous therapy with acyclovir
(60 mg/kg/day)
Antibody Therapy
Antibodies against the surface glycoproteins gB and gD prophylactic and therapeutic agents in HSV infection
inactive acyclovir
Viral thimydin kinas
Monophosphte acyclovoir
cellular thimydin kinase
active )) acyclovir three phosphate
mechanism acyclovir
is analog to guanin blocked DNApolymerasefunction
PREVENTION
Cesarean Deliverycesarean delivery in a woman with activegenital lesions can reduce risk of acquiring HSV to infant
Antiviral Prophylaxis During Pregnancythe use of oral acyclovir near the end of pregnancy to suppress genital HSV Because of acyclovir’s safety
HSV-2 : glycoprotein D subunit
vaccine adjuvanted :monophosphorylipid A(MPL)
Hsv vaccine
efficacy was limited to women who were HSV-1 and -2 seronegative before receiving vaccination.
Thank you for your Attention