Gut-Brain Regeneration:The microbiome as a new avenue for

therapeutic management

Zach Bush, MDInternal Medicine, Endocrinology and Metabolism

Founder, Director of Clinical AffairsRevolution Health Center

Scottsville, VA

Founder, CEOBiomic Sciences

Disclosure• I discovered carbon-based bacterial metabolites that

have the potential to function as Redox signaling molecules

• I am founder and CEO of Biomic Sciences that has brought to market RESTORE, the first Carbon-based redox molecule supplement, and my team has created a new paradigm of cell signaling science

• I am the founder and President of Revolution Health Center

• My clinical and basic science operations and experience that I share with you today produce all of my income

USA Ranks 49th in health outcomes

1st World Epidemics• Autism 1:68• Attention Deficit 1:10 (70% medicated)• Asthma 1:10• Allergy 1:4• Diabetes 1:4• Obesity 1:3• Major Depression 1:2• Cancer 1:2• Dementia 1:1

1st World Epidemics• Autism 1:68• Attention Deficit 1:10 (70% medicated)• Asthma 1:10• Allergy 1:4• Diabetes 1:4• Obesity 1:3• Major Depression 1:2• Cancer 1:2• Dementia 1:1

1st World Epidemics• Autism 1:68• Attention Deficit 1:10 (70% medicated)• Asthma 1:10• Allergy 1:4• Diabetes 1:4• Obesity 1:3• Major Depression 1:2• Cancer 1:2• Dementia 1:1

Root Cause of disease?

Root Cause of Aging and Disease

Inflammation is the root of all chronic disorder and disease

Autism

Oxidative Stress

• Acute inflammation can save our lives – Injury Repair– Infection control

• Chronic inflammation– oxidative stress = positive charge/acid– Shutdown/overwhelm of the antioxidant system

chronic inflammation = loss of communication

What is theRoot Cause of Inflammation?

Root Cause of Aging and Disease

Ecosystem News

• Bacterial population patterns in the human gut predict occurrence of chronic inflammatory disease

– Obesity/Diabetes– Asthma– Autoimmunity– Cancers– Dementia– Autism

The GALT

• Gastrointesinal Associated Lymphatic Tissue

GALT: 80% of the antibodies in your body are made here

Gut InflammationFood SensitivityAsthma/EczemaAllergyAutoimmune

Intercellular Tight JunctionsFirewalls of Defense

Gut/Brain Axis

• Gut injury: Gluten, Glyphosate, Lipopolysaccharides

• Zonulin goes systemic• Tight junction injury

– Gut– Blood/brain barrier– Vascular system– Kidney tubules

Gut/Brain Axis

• Leaky Brain– Interleukin inflammatory cytokines– Neuro transmitter dysregulation– Neuro-hormonal stress

A catastrophic vulnerability

Bacteria

UnprotectedMembranes

Tight Junction Toxins

Bacteria

Bacterial metabolites:Carbon-based Redox system

• Each bacterial species (30,000 species) creates a unique subset of carbon metabolites during the digestive process

• These function as an interspecies molecular communication network that allows for coordinated cell protection, injury response, and repair

Carbon Redox

www.restore4life.com

What are your bacteria saying?

• Dysbiosis: broken ecosystem– Monoculture Antibiotics, pesticides, chemicals, processed foods

• Weeds grow up: E.Coli, Klebsiela, Psudomonas, Candida

Probiotics – 3 - 24 species, $30 Billion monoculture

Monoculture = limited vocabulary = limited defense

• Optimal bowel ecology = 20,000 species

Biodiversity = fluid communication = strong defenes

GLUTENToxic Peptides

CXCR3

InflammationZONULIN

ENZ

TJ breakdown

Leaky Gut/Brain Leak GutGLYPHOSATE

BACTERIAL/YEASTOVERGROWTH

Enzymes

Chronic Inflammation

Leaky Gut

Vagal Nerve Stimulation and CNS inflammation

Functional Gut Barrier

www.restore4life.com

Leaky Gut in real time

VEH PT-GliadinCONTROL Gluten in 1 slice of pizza

www.restore4life.com

Bacterial Communication Frontlines of Defense

TIGHT JUNCTIONS

Bacterial communication impacts tight junction function

TE

ER

ohm

s/cm

2

www.restore4life.com

From Sieve to FirewallDouble-click to add an object

VEH PT-GliadinCONTROL GLUTEN

GLUTEN and RESTORE RESTORE ww

w.restore4life.com

Bacterial communication induces differential

Mitochondrial ROS ActivityR

OS

pro

duct

ion

www.restore4life.com

ROS assay RT- PCR

1 3 5 10 60

Minutes

DAMAGED

(MCF-7)

HEALTHY

Bacterial communication impacts Stem Cell Activation

Urin

e C

D13

3

www.restore4life.com

Urine CD133

1st and 2nd birth of our children

HUMAN70 Trillion Cells 25,000 Genes

BACTERIA 20,000 Species

1.4 Quadrillion Cells 2 Million Genes

Bacteria

www.restore4life.com

Epigenetics / Micro RNA

• Which genes turn on/off is determined by your environment

• Micro RNA are non-coding sequences in the genome that turn on and off gene pathways at the DNA level

• 15% of the micro RNA that is circulating in your blood stream today are from the bacteria in your gut– Bacterial genome is turning on and off the human

genes

www.restore4life.com

USA Ranks 49th in health outcomes

www.restore4life.com

The natural history of neurologic injury

• Children are being born into an ever more narrow bacterial ecosystem (vulnerability)

• Breast milk and food/drink thereafter is contaminated with glyphosate-based herbicides

• Gluten, polyunsaturated oils, and other refined foods

(leaky gut/leaky brain occurs)• Exposure to antibiotics, NSAIDS, Alcohol, invasive bacteria,

parasites• Leaky Gut/Leaky Gut Brain – Toxins accumulate

(accelerating neurologic injury)• Widespread immune dysfunction (invasive bacteria, chronic

viremia – HPV, HSV, HepC, HIV)• Shutdown of the antioxidant system (chronic inflammation)• System overwhelm (Autism to Alzheimers, CVD to Cancer)

Leaky Gut - Leaky BrainInflammation cascade from birth to

death Colic

Sensory processing defects Attention Deficit Anxiety Disorders Major Depression

Disordered Sleep Insulin resistance Infertility Diabetes Vascular Disease Cancer Dementia

Gut/Brain Injury What can we do about it?• Tight Junction firewall up and running• Direct toxin antidote – glyphosate, gluten• Promote eco-diversity in the gut and beyond• Increase bacterial breakdown of toxins• Increase bio-availabilty of food nutrients

Maximize the Biome: Get out in nature – breathe biodiversity Grow your own food Eat from the bottom of the food chain Stop Dairy (high glyphosate, highly acidic) Fermented foods:

Miso Soup, Sauerkraut, etc

www.restore4life.com

Soil/Gut Communication Supplementation:

What’s in Restore?• Carbon-based redox molecules extracted from fossil soil

in the South West United States• Trace micronutrients – minerals and amino acids with

concentration profiles similar to that of a sweet potato skin

• Unprecedented results in cellular safety studies– Gold-standard Renal Tubule Toxicity studies – No LD50, extends

life of cell lines at every concentration– Even at 100% concentrations

Restore Logistics• 1 tsp to 1 TBL three times daily before or with

meals• For very sensitive kids:

– 2-5 drops by mouth – plain or

with non-chlorinated water/juice– Slowly increase to 1 tsp three times daily

• Once on restore you can stop probiotics and digestive enzymes to speed recovery of natural gut biome diversity and healthy digestive processes

• No drug-drug interactions

References1. Centers for Disease Control and Prevention (CDC), Key Findings: Trends in the

Parent-Report of Health Care Provider-Diagnosis and Medication Treatment for ADHD: United States, 2003-2011 the National Survey of Children's Health (NSCH)

2. Ellwood P, Williams H, Aït-Khaled N, Björkstén B, Robertson C, ISAAC Phase III Study Group. Translation of questions: The International Study of Asthma and Allergies in Childhood (ISAAC) experience. Int J Tuberc Lung Dis. September 2009; 13(9): 1174-1182

3. Massey JT, Moore TF, Parsons VL, Tadros W. Design and estimation for the National Health Interview Survey, 1985–1994. National Center for Health Statistics. Vital Health Stat 1989;2(110)

4. Botman SL, Moore TF, Moriarity CL, Parsons VL. Design and estimation for the National Health Interview Survey, 1995–2004. National Center for Health Statistics. Vital Health Stat 2000;2(130)

5. Centers for Disease Control and Prevention. National Diabetes Statistics Report: Estimates of Diabetes and Its Burden in the United States, 2014. Atlanta, GA: US Department of Health and Human Services; 2014

References6. Janeway, CA Jr.; et al. (2001). "The mucosal immune system". Immunobiology. New

York: Garland Science. 10-13

7. Salminen S, Bouley C, Boutron-Ruault MC, et al. (1998). "Functional food science and gastrointestinal physiology and function". British Journal of Nutrition 80 (S1): S147–S171

8. Moriame G; et al. Viral Suppression and Immune Restoration in the Gastrointestinal Mucosa of HIV Type 1-Infected Patients Initiating Therapy during Primary or Chronic Infection Journal of Virology, August 2006, p. 8236-8247, Vol. 80, No. 16

9. Abbas A.B.; Lichtman A.H. (2009). "Ch.2 Innate Immunity". In Saunders (Elsevier). Basic Immunology. Functions and disorders of the immune system (3rd ed.)

10. Eming, S. A.; Krieg, T.; Davidson, J. M. (2007). "Inflammation in wound repair: molecular and cellular mechanisms". Journal of Investigative Dermatology 127 (3): 514–525

11. Van Dorpe, S; et al. (2012). "Brainpeps: The blood–brain barrier peptide database". Brain structure & function 217 (3): 687–718

12. Schneider, Stefan W.; et al. (March 2004). "Glioblastoma cells release factors that disrupt blood–brain barrier features". Acta Neuropathologica 107 (3): 272–276

References13. H Chen; EE Konofagou. Journal of Cerebral Blood Flow &

Metabolism (2014) 34, 1197–1204

14. H Matsui ; et al. (2011). The pathophysiology of non-steroidal anti-inflammatory drug (NSAID)-induced mucosal injuries in stomach and small intestine J Clin Biochem Nutr. 2011 Mar; 48(2): 107–111

15. Eadon MT; et al. Endotoxemia alters tight junction gene and protein expression in the kidney (2012). Am J Physiol Renal Physiol. 2012 Sep 15; 303(6): F821–F830