Skin Microbiome
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Transcript of Skin Microbiome
Skin MicrobiomeElizabeth Grice, PhD
Assistant Professor, Dermatology & MicrobiologyUniversity of Pennsylvania
The ecosystem of the skin• Terminally differentiated keratinocytes (corneocytes)• Average skin temp = 32-33 C• Varied topography: thickness, hair, glands, occlusion
Mary Marples, The Ecology of the Human Skin, 1965
Grice et al. (2011) Nature Reviews Microbiology
Topography of the skin microbiome
Oh et al. (2014) Nature.
Kingdom‐level diversity of the skin microbiome
K Findley et al. Nature (2013)
Fungal diversity of the skin
The skin virome: lots of “dark matter”
Hannigan et al. mBio 2015 and Hannigan et al. under review
Umbilicus
Toeweb
Scalp
Behind ear
Palm
Forehead
Axilla
Inner elbow
The skin microbiome is surprisingly stable over time
Oh et al. (2016) Cell.
• Generally, same dominant bacteria are present between individuals• The remainder is highly variable
Grice and Segre (2011) Nature Reviews MicrobiologyData from Grice et al (2009) Science
Skin microbiome interpersonal variability
Signatures of individuality are low abundance, shared across sites
Oh et al. (2014) Nature.
Meisel et al J of Investigative Dermatology, 2016 Jackie Meisel
Benchmarking best practices for skin microbiome characterization
Mock community analysis to compare taxonomic result of sequencing approach
Meisel et al J of Investigative Dermatology, 2016
V4 primers exclude Propionibacterium
Meisel et al. J. of Invest. Derm. 2016
V1V3 region allows for increased phylogenetic placement of Staphylococcus species
Meisel et al. J. of Invest. Derm. 2016
Skin Microbiome Summary• Skin is a multi-niche habitat that supports the colonization and
growth of microbes;• Skin site is an important determinant of the dominant colonizing
bacteria and diversity;• Signatures of individuality are apparent in the lower abundance
members;• Temporal diversity is surprisingly stable despite constant
environmental exposures and perturbations;• Non-bacterial members of the skin microbiota are still being
resolved, but are important components to consider;• Optimal approaches for skin microbiome characterization may
be different than other body sites and/or the environment.
Lab members:Jacquelyn MeiselAdam SanMiguelCharles Bradley, VMDMichael LoescheJoseph HorwinskiQi Zheng, PhDCasey Bartow-McKenneyJulia BugayevGeorgia Sfyroera PhDLindsay Kalan PhDGeoffrey Hannigan PhD (former)
Funding:NIAMS R01AR066663NINR R01NR015639Janssen Pharmaceutical CompaniesPennsylvania Department of Health
Collaborators:Sue Gardner RN PhD (U of Iowa Nursing)David Margolis MD PhD (Penn Derm & Epidemiology)Rick Bushman PhD (Penn Microbiology)Samir Mehta MD (Penn Orthopaedic Surgery)Junko Takeshita MD (Penn Derm)John Lambris PhD (Penn Pathology and Lab Medicine)Kara Spiller PhD (Drexel Biomedical Engineering)Phil Scott PhD (Penn Vet Medicine)Meghan Davis DVM MPH (Johns Hopkins SPH)Dan Morris DVM MPH (Penn Vet Medicine)Elizabeth Mauldin DVM (Penn Vet Medicine)