GRAM STAINING Dr.T.V.Rao MD 1. Hans Christian Joachim Gram Danish Bacteriologist Dr.T.V.Rao MD2.
Gram Staining
13
Microbiology laboratoric test
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Transcript of Gram Staining
Gram Stain• Material
– solutions of crystal violet, Gram’s iodine (2 g potassium iodide in 300 ml distilled water plus 1 g iodine crystals), 95% ethanol and/or
– isopropanol-acetone mixture (3:1 v/v), and
– safranin– Bismark brown stain (for color-
blind students)– clean glass slides– inoculating loop– Bunsen burner– bibulous paper
– microscope– lens paper and lens cleaner– immersion oil– Hyphomonas (Hyphomicrobium)
neptunium (ATCC 15444) grown in marine broth (Difco)
– slide warmer– staining rack– Bacto Gram Stain Reagents from
Difco for the three-step Gram stain