Observation of bacteria using staining procedures Simple staining Gram staining.
The Three-Domain System · . Gram positive cell wall Membrane protein. Gram negative cell wall 25....
Transcript of The Three-Domain System · . Gram positive cell wall Membrane protein. Gram negative cell wall 25....
fimbriaglycocalyx
pilus
flagellum
Cell wall
Cell membrane
ribosome
Outer membrane
Actin cytoskeleton
Bacterial chromosome or nucleoid
Inclusion/ granule
Bacterial cell
SIMPLE STAINING:-
METHYLENE BLUE staining IMPART SAME COLOUR TO ALL BACTERIA
USED TO KNOW THE ARRANGEMENT
GRAM STAINING
GRAM POSITIVE BACTERIA - VIOLET STAPHYLOCOCCUS STREPTOCOCCUS
GRAM NEGATIVE BACTERIA - PINK E.COLI KLEBSIELLA
ACID FAST STAINING ZEIHL-NEELSON METHOD
PINK COLOR BACILLI
TUBERCLE BACILLI
LEPRA BACILLI BACTERIAL SPORES, CRYPTOSPORIDIUM
Endospore formation
Vegetative cell
Vegetative cell becomessporangium in preparation forSporulation
Sporangium
Sporangium withprospore
1.Germinal groove
2.Outer cortical layer
3.Cortex
4.Inner spore coat
6.Outer spore coat
Bacterial spore
BACTERIAL GROWTH CURVE
Lag phase - with no increase in number ( adaptation of new surrounding)
Log phase - phase of division
Stationary phase - division stops due to depletion of nutrients.Phase of decline - due to cell death.
A = lag phase,B = acceleration phase,C = log (exponential) phase
D = stationary phase, E = death phase.