GL SCIENCES INERTSIL ODS-2 - ACORE Consumíveis · INERTSIL® ODS-2 An HPLC column offering...
2
INERTSIL ® ODS-2 An HPLC column offering symetric peaks for polar bases and acids and low pressure. GL SCIENCES HPLC COLUMNS GL Sciences’ Original High Purity ODS Phase When Inertsil ODS-2 was introduced in the late 1980s, this HPLC transformed the entire industry. Inertsil ODS-2 was the first HPLC phase created using ultra high purity silica, which produced superior base deactivation. Additionally, a unique “oligomeric” bonding architecture created a novel selectivity and a degree of chemical and physical ruggedness as yet unseen. Until this phase was eclipsed by the performace of it’s sibling Inertsil ODS-3 (and now, by Inertsil ODS-4), it was GL Sciences’ most popular phase and continues to be used widely and reliably for long established methods in pharmaceutical and environmental labs. While Inertsil ODS-2 is generally recommended primarily for following prescribed methods, (Inertsil ODS-4 and ODS-3 being recommended for new methods) it constitutes of one of six Inertsil ODS phases offering alternative selectivities for polar compounds. For additional information, please visit www.inertsil.com. Features & Benefits • Extremely reproducible. • Oligomeric bonding yields unique selectivity • Standard dimensions always in stock. Phase Characteristics Particle Size: 5um Particle Shape: Spherical Specific Surface Area: 320m2/g Pore Size: 150A Pore Volume: 1.15mL/g Purity: 99.999% Bonded Phase: Octadecyl groups Endcapped: Yes Carbon Load: 18.5% USP: L1 Guaranteed Efficiency: 80,000 p /m Pure Reverse Phase Chromatography The absence of metals and other impurities in Inertsil ODS-2 silica, combined with robust end-capping allows pure reverse phase chromatography for even the most polar compounds. The chromatograms at left show selectivity differences between Inertsil ODS-2 and GL Sciences’ more advanced ODS phases. Selectivity Comparison between Inertsil ODS Phases using PAHs and Alkylbenzenes Columns: As indicated, 250 x 4.6mm; Eluent: MeCN: 25mM Phosphate Buffer, pH 7.0, 40:60 Flow Rate: 1.0 mL/min Temp: 40C Detecon: 210nm Sample: Uracil, Caffeine, Phenol, n-Butylbenzene, o-Terphenyl, n-Amylbenzene, Triphenylene ODS-4 ODS-3 ODS-SP ODS-2 ODS-EP ODS-P 1 +2 3 4 7 5 6 1 +2 3 4 7 5 6 1 +2 3 4 7 5 6 1 2+3 4 7 5 6 1 +2 3 4 7 5 6 1 +2 3 4 7 5 6 Inertsil ODS Phase Selectivity Comparison
Transcript of GL SCIENCES INERTSIL ODS-2 - ACORE Consumíveis · INERTSIL® ODS-2 An HPLC column offering...
INERTSIL® ODS-2An HPLC column offering symetric peaks for polar bases and acids and low pressure.
GL SCIENCESHPLC COLUMNS
GL Sciences’ Original High Purity ODS PhaseWhen Inertsil ODS-2 was introduced in the late 1980s, this HPLC transformed the entire industry. Inertsil ODS-2 was the first HPLC phase created using ultra high purity silica, which produced superior base deactivation. Additionally, a unique “oligomeric” bonding architecture created a novel selectivity and a degree of chemical and physical ruggedness as yet unseen. Until this phase was eclipsed by the performace of it’s sibling Inertsil ODS-3 (and now, by Inertsil ODS-4), it was GL Sciences’ most popular phase and continues to be used widely and reliably for long established methods in pharmaceutical and environmental labs. While Inertsil ODS-2 is generally recommended primarily for following prescribed methods, (Inertsil ODS-4 and ODS-3 being recommended for new methods) it constitutes of one of six Inertsil ODS phases offering alternative selectivities for polar compounds. For additional information, please visit www.inertsil.com.
Features & Benefits • Extremely reproducible.
• Oligomeric bonding yields unique selectivity
• Standard dimensions always in stock.
Phase Characteristics
Particle Size: 5um Particle Shape: SphericalSpecific Surface Area: 320m2/gPore Size: 150APore Volume: 1.15mL/gPurity: 99.999%Bonded Phase: Octadecyl groupsEndcapped: YesCarbon Load: 18.5%USP: L1Guaranteed Efficiency: 80,000 p /m
Pure Reverse Phase Chromatography
The absence of metals and other impurities in Inertsil ODS-2 silica, combined with robust end-capping allows pure reverse phase chromatography for even the most polar compounds.
The chromatograms at left show selectivity differences between Inertsil ODS-2 and GL Sciences’ more advanced ODS phases.
Selectivity Comparison between Inertsil ODS Phases using PAHs and Alkylbenzenes Columns: As indicated, 250 x 4.6mm; Eluent: MeCN: 25mM Phosphate Buffer, pH 7.0, 40:60 Flow Rate: 1.0 mL/min Temp: 40C Detection: 210nm Sample: Uracil, Caffeine, Phenol, n-Butylbenzene, o-Terphenyl, n-Amylbenzene, Triphenylene
ODS-4
ODS-3
ODS-SP
ODS-2
ODS-EP
ODS-P
1 +2
3 4
7
5
6
1 +2
34
75
6
1 +2
34
7
5
6
1 2+3
4
7
5
6
1 +2
34
7
5
6
1 +2
34
7
5
6
Inertsil ODS Phase Selectivity Comparison
Components of Cold Drugs
Column: Inertsil ODS-2, 5 micron, 150 x 4.6mm; Eluent: A: MeOH/0.1% H3PO4 30/70B: MeOH/0.1% H3PO4 80/20; Gradient: 0%B to 100%B in 15 min; Flow: 1.0 mL/min;Temp: 40C; Detector: UV at 220nm; Sample: 1. acetaminophen, 2. caffeine, 3. ethenzamide, 4. bucetin, 5. acetyl salicylic acid
Ordering InformationUSA Part No. Phase Particle Size Column Dimensions Japan Part No.1210L150W021 Inertsil ODS-2 5 micron 150 x 2.1mm 5020-01101-211210L250W021 Inertsil ODS-2 5 micron 250 x 2.1mm 5020-01102-211210L150W030 Inertsil ODS-2 5 micron 150 x 3.0mm 5020-01101-301210L250W030 Inertsil ODS-2 5 micron 250 x 3.0mm 5020-01102-301210L100W046 Inertsil ODS-2 5 micron 100 x 4.6mm 5020-ODS-46-1001210L150W046 Inertsil ODS-2 5 micron 150 x 4.6mm 5020-01101-461210L250W046 Inertsil ODS-2 5 micron 250 x 4.6mm 5020-112101210P1KIT040 Inertsil ODS-2 5 micron 2 x 10 x 4.0mm guard cartridges with holder 5020-08520-ODS21210P010W040 Inertsil ODS-2 5 micron 2 x 10 x 4.0mm replacement guard cartridges 5020-08510-ODS2This is only a partial listing. Please visit www.inertsil.com for a complete listing or inquire by phone at the number shown below.
GL Sciences, Inc. USA4733 Torrance Blvd., Suite 255, Torrance, CA 90503Tel 310-265-4424 Fax 310-265-4425 Web www.inertsil.com
Column Particle Surface Pore Silica Carbon ODS Phase Degree of Name Size (µ) Area (m2/g) Size (Å) Purity (%) Load (%) Type / Structure End-Capping Inertsil ODS-2 5 320 150 99.999 18.5 Oligomeric Well Inertsil ODS-4 2,3,5 450 100 99.999 11 Monomeric Extreme Inertsil ODS-3 2,3,4,5 450 100 99.999 15 Monomeric Well Inertsil ODS-EP 5 450 100 99.999 9 Polar Embedded None Inertsil ODS-SP 5 450 100 99.999 8.5 Monomeric Well Inertsil ODS-P 5 450 100 99.999 29 Polymeric None
1
3
4
2
5
Guard Cartridges for Inertsil ODS-2 Columns
Wide Range of Inertsil ODS-2 Column Dimensions Available
Daniel
Acore Tel
