Inertsil ODS-EP Technical Information · Comparison of inertness to oxine-copper between Inertsil...

Inertsil ODS-EP Technical Information

Advantages of Inertsil ODS-EP

● The selectivity is completely different from those of conventional

columns such as ODS column due to its specific polar group in the

stationary phase.

● Durability can be obtained by the high density bonding.

● Superior peak shapes of both acids and bases can be obtained because of

neutral bonded phase and low residual silanol groups.

● Silica gel of high-purity is used as the support material, which

minimizes effects of any remaining metal ions.

● Specifications of Inertsil ODS-EP

Support Silica Gel Chemical Modification

Partical

diameterShape

Specific

surface area

Pore

diameterPurity Bonded group

ODS Carbon

content

Inertsil ODS-EP 5μｍ Spherical 450ｍ２ /g 100Å 99.999Embededpolar

Octadecyl group９ %

(1) Naphthalene sample

~ for measurement retention and peak shape ~

Conditions

Column dimensions:

4.6 mm I.D x 150 mm length

Mobile phase:

Acetonitrile : Water =65 : 35

Flow rate: 1 ml / min

Column temperature: 40℃Detection: UV 254 nm

Inertsil ODS-EP

Peak identification

1. Acetophenone

2. Benzene

3. Toluene

4. Naphthalene

0 2 4 6 8

Time (min)

1

2

3

4

0 2 4 6

Time (min)

Symmetry Shield RP18

0 2 4 6

Time (min)

Xterra RP18

0 2 4 6

Time (min)

Discovery RP Amide C16

0 2 4 6

Time (min)

Zorbax Bonus-RP

0 2 4 6

Time (min)

Hypersil HyPURITY Advance

Inertsil ODS-EP

Comparison of retention between Inertsil ODS-EP and commercial

embedded columns

0 2 4 6

Time (min)

0 2 4 6

Time (min)

Aqua C18(polar endcapping)

0 2 4 6 8

Time (min)

Inertsil ODS-3

Inertsil ODS-EP

0 2 4 6

Time (min)

Inertsil C8-3

0 2 4 6

Time (min)

Inertsil Ph-3

Comparison of retention between Inertsil ODS-EP and other type

commercial columns

0 2 4 6

Time (min)

(2) Selectivity test sample ~ for measurement selectivity ~

Conditions

Column dimensions:


Mobile phase:

Acetonitrile : Water = 70 : 30



Inertsil ODS-EP

Peak identification

1. Ethylbenzene

2. Naphthalene

3. n-Propylbenzene

4. n-Butylbenzene

5. Anthracene

(CH2)3CH3

C2H5

(CH2)2CH3

0 2 4 6 8 10

Time (min)

1

2

34

5

0 2 4 6 8 10

Time (min)


Xterra RP18


Zorbax Bonus-RP


Inertsil ODS-EP

Comparison of selectivity between Inertsil ODS-EP and commercial

embedded columns

0 2 4 6 8

Time (min)

n-Butylbenzene

Anthracene

0 2 4 6 8

Time (min)

n-Butylbenzenen-Butylbenzene

n-Butylbenzene

Anthracene

Anthracene

0 2 4 6 8

Time (min)

n-Butylbenzene

+ Anthracene

0 2 4 6 8

Time (min)

n-Butylbenzene

+ Anthracene

0 2 4 6 8

Time (min)

Anthracene


Inertsil ODS-3

Inertsil ODS-EP

Inertsil C8-3

Inertsil Ph-3

Comparison of selectivity between Inertsil ODS-EP and other type

commercial columns

n-Butylbenzene

Anthracene

0 2 4 6 8 10 12

Time (min)

0 2 4 6 8 10 12

Time (min)

0 2 4 6 8

Time (min)

0 2 4 6 8

Time (min)

n-Butylbenzenen-Butylbenzene

n-Butylbenzene

n-Butylbenzene

Anthracene

Anthracene

Anthracene

Anthracene

0 2 4 6 8 10

Time (min)

The selectivity of Inertsil ODS-EP is completely different from

those of conventional columns such as ODS column due to its

specific polar group in the bonded phase.

Comparison of retention and selectivity between Inertsil ODS-EP and commercial embedded columns

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

10.00

0.80 1.00 1.20 1.40

α（Anthracene/Butylbenzene）

k' (n-

But

ylbe

nzen

e)

Inertsil ODS-EP


Xterra RP18


Zorbax Bonus-RP


Inertsil ODS-3

Inertsil C8-3

Inertsil Ph-3

Aqua C18

Inertsil ODS-EP

0 10 20 30

Time (min)

(3) Tanaka-test sample

~ for measurement selectivity, hydrogen bonding capacity

and hydrophobicity ~

Conditions

Column dimensions:


Mobile phase:

Methanol : Water = 80 : 20



Inertsil ODS-EP

Peak identification

1. Uracil

2. Caffeine

3. Phenol

4. n-Butylbenzene

5. n-Amylbenzene

6. o-Terphenyl

7. Triphenylene

1

2

3

45

67

0 10 20 30

Time (min)


Xterra RP18


Zorbax Bonus-RP


Inertsil ODS-EP

0 10 20

Time (min)

0 2 4 6 8 10

Time (min)

0 2 4 6 8 10

Time (min)

0 2 4 6 8 10

Time (min)

0 2 4 6 8 10

Time (min)

１

２

３

４５６７

１

２

３４

５６

７

１

２

３４５６

７

１

２

３４５６

７

１＋２

３４５+６

７

１

２３

４５６

７

Comparison of selectivity between Inertsil ODS-EP and commercial

embedded columns

0 10 20 30

Time (min)


Inertsil ODS-3

Inertsil ODS-EP

Inertsil C8-3

Inertsil Ph-3

Comparison of selectivity between Inertsil ODS-EP and other type

commercial columns

0 10 20

Time (min)

0 2 4 6 8 10 12 14

Time (min)

0 2 4 6 8 10 12 14

Time (min)

0 2 4 6 8 10

Time (min)

１

２

３４５

６７

１

２

３４５６

７

１

２

３

４

５+６７

１

２

３４５６

７

１

２３

４５

６+７

0.00

0.10

0.20

0.30

0.40

0.50

Inertsil ODS-EP Symmetry Shield

RP18

Xterra RP18 Discovery RP

Amide C16

Zorbax Bonus-RP Hypersil HyPURITY

Advance

α（caffeine/ph

enol）

Hydrogen bonding capacity

Hydrophobicity

Steric selectivity

Characterization of Inertsil ODS-EP and commercial embedded columns by Tanaka test

(1) Caffeine shows no retention

(1)

Conditions

Column dimensions:


Mobile phase:

Methanol : Water = 80 : 20



Phenol

　　　　　Ｏ　　　ＣＨ３

CH3　　　　　　　　Ｎ

　 N

　　　　　　　　　　　　Ｎ

　Ｏ　　　Ｎ

　　　　　 CH3

　　　　　　　　（ＣＨ２）４ CＨ３　　　　　　　（ＣＨ２）３ＣＨ３

　　　　ＯＨ

Triphenylene

n-Amylbenzene

o-Terphenyl

n-Butylbenzene

Caffeine

0.00

0.50

1.00

1.50


RP18


Amide C16

Zorbax Bonus-RP Hypersil

HyPURITY

Advance

α（a

mylbe

nzen

e/bu

tylben

zene

）

0.00

0.50

1.00

1.50

2.00

2.50

3.00


RP18


Amide C16

Zorbax Bonus-RP Hypersil

HyPURITY

Advance

α（trip

heny

lene

/o-terph

enyl）

(4) Pyridine-Phenol sample

~ for measurement of inertness to basic compounds ~

Conditions

Column dimensions:


Mobile phase:

Methanol : 10mM Phosphate buffer(pH6.8)

=30 : 70



Inertsil ODS-EP

Peak identification

1. Uracil

2. Pyridine

3. Phenol0 2 4 6 8 10 12 14

Time (min)

1

2

3


Xterra RP18


Zorbax Bonus-RP


Inertsil ODS-EP

Comparison of inertness to pyridine between Inertsil ODS-EP and

commercial embedded columns

0 2 4 6 8 10 12

Time (min)

0 2 4 6 8 10 12

Time (min)

0 2 4 6 8 10 12

Time (min)

0 2 4 6 8 10 12

Time (min)

0 2 4 6 8 10 12

Time (min)

Pyridine

Phenol

Pyridine Phenol

Pyridine

Phenol

Pyridine Phenol

Pyridine

Phenol

Pyridine

Phenol

0 2 4 6 8 10 12 14

Time (min)

(5) Acid test sample

~ for measurement of inertness to acidic compounds ~

Conditions

Column dimensions:


Mobile phase:

0.1% Phosphoric acid(pH2.2)



Inertsil ODS-EP

Peak identification

1. Formic acid

2. Acetic acid1.0 2.0 3.0 4.0 5.0

Time (min)

1

2

1.0 2.0 3.0 4.0 5.0

Time (min)


Xterra RP18


Zorbax Bonus-RP


Inertsil ODS-EP

1.0 2.0 3.0 4.0 5.0

Time (min)

1.0 2.0 3.0 4.0 5.0

Time (min)

1.0 2.0 3.0 4.0 5.0

Time (min)

1.0 2.0 3.0 4.0 5.0

Time (min)

1.0 2.0 3.0 4.0 5.0

Time (min)

Formic acid

Acetic acid

Formic acid

Formic acid

Formic acid

Acetic acid

Acetic acid

Acetic acid

Formic acid

+Acetic acid

Acetic acid

Comparison of inertness to acids between Inertsil ODS-EP and


(6) Oxine-copper sample

~ for measurement of inertness to chelating compounds ~

Conditions

Column dimensions:


Mobile phase:

Acetonitrile : 0.1% Phosphoric acid(pH2.2)

=10 : 90



Inertsil ODS-EP

Peak identification

1. Oxine-copper1.0 2.0 3.0

Time (min)

1


Xterra RP18


Zorbax Bonus-RP


Inertsil ODS-EP

Comparison of inertness to oxine-copper between Inertsil ODS-EP and


1.0 2.0 3.0

Time (min)

1.0 2.0 3.0

Time (min)

1.0 2.0 3.0

Time (min)

1.0 2.0 3.0

Time (min)

1.0 2.0

Time (min)

1.0 2.0 3.0

Time (min)

0

20

40

60

80

100

0 50 100Flow time of 0.1% Phosphoric acid (pH2) ( hours )

Rel

ativ

e re

tent

ion

tim

e of

naph

thalen

e（％

）

Inertsil ODS-EP

ODS test column

Amide test column

Comparison of durability among Inertsil ODS-EP, ODS column

and Amide column

Test condition

Column: 4.6 mm I.D. x 150 mm


Column temperature: 30℃

Inertsil ODS-EP

● The selectivity is completely different from those of conventional

columns such as ODS column, but the retention is close to conventional

columns.

● High durability can be obtained even if strong acid was used as mobile

phase.

● Superior peak shapes of both acids and bases can be obtained because of

neutral bonded phase and low residual silanol groups.

● Silica gel of high-purity is used as the support material, which

minimizes effects of any remaining metal ions.

Inertsil ODS-EP is useful for the samples which are not separated by

conventional columns.

Inertsil ODS-EP is useful for the LC/MS analysis.

Inertsil ODS-EP Technical Information · Comparison of inertness to oxine-copper between Inertsil...

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