Pennsylvania Highlands Community College

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Fall 2012

Pennsylvania Highlands Community College

Index

Welcome…………………………………………………………………………..1

Frequently Asked Questions …………………………………………………….2

Pennsylvania Highlands Community College

Welcome

Quick Contact List for New Faculty

Name Title Contact

William Wolff Assistant Professor, Chemistry

262-6479

Jill Mitchell Instructor, Biological Sciences Lead Faculty

262-6487

Ashley Hollern Instructor, Biological Sciences 262-6412

We want to first welcome you to the Biology Department, here at Pennsylvania Highlands Community College. This packet includes information that will be useful in your faculty role as an instructor. This is designed to make your transition into the Biology Department at Pennsylvania Highlands Community College more pleasant and productive. The Biology department provides a solid foundation in science by instilling in students a commitment to search for truth through application of the scientific method, to gain a better understanding of humans, the earth and the universe, and to desire knowledge that can be applied to the service of others. The department offers courses that fulfill the college's Core Curriculum, as well as courses that support the Biotechnology Program. Your role as a member of the department is to aid our endeavors of creating a learning environment that enhances critical thinking, allows for collaboration, and exploration through experience which lends to life-long learning skills.

WELCOME TO PENNSYLVANIA HIGHLANDS

COMMUNITY COLLEGE BIOLOGY DEPARTMENT

Summer 2011

Volume 1,

Issue 1

Important Phone Extensions

Security – x 6247 Security Emergency – x 5555 IT Help Desk – x 6470 Front Desk – x 6400

To make a local call from any room

dial 9 first.

101 Community College Way, Johnstown PA 15904

www.pennhighlands.edu 814-262-6400

Wolff, William, Associate Professor, Natural Sciences and Technology Mitchell, Jill, Instructor, Biological Sciences Hollern, Ashley, Instructor, Biological Sciences

Pennsylvania Highlands Community College

1. How do I obtain the keys for my classroom?

The keys for your lecture are lab room can be obtained from Mary Ann Lee during the

hours of 8:30am and 4:30pm. If your class is held after these hours you should contact

security at x6427 which can be dialed from any open classroom or from the faculty copy

room.

2. Who are the full time departmental employees in biology?

Jill Mitchell is the Biology Department lead faculty. She teaches BIO110, and BIO115.

Her office is located in C. Ashley Hollern is a full time biology faculty member and

teaches BIO210, BIO260, and BIO262. Her office is located in C

3. Who can I ask for assistance while teaching lecture / lab?

Feel free to contact either Jill Mitchell at x 6487 or Ashley Hollern at x 6412 with any

questions or concerns regarding lab or lecture questions.

4. What supplies are available to me?

You will find a list of supplies available to you in this manual. See pages ****

5. Is it ok to borrow supplies from another lab cabinet?

We would prefer you obtain the lab supplies from the lab cabinet assigned to your class.

However, if supplies are in short supply and you must borrow supplies from another

cabinet, please make a note of the need for a purchase order. There is a tablet for

“supplies needed” that can be found in C230B Storage room, or C226 B Storage room.

6. Where do I make copies?

Copies can be made in the faculty copy room located in C*****

7. Who do I contact if I need additional supplies?

If you are aware of the need for additional supplies at the beginning of the semester, you

should contact Jill Mitchell with the Supply list, company name and purchase order

information so that the order may be processed in a timely manner.

8. How do I address supplies that I run out of during the semester?

If you should run out of supplies during the semester and no replacements are available,

contact Jill Mitchell ASAP so that an order may be processed.

9. What is the proper PHCC procedure for disposing of laboratory material?

You will find a “Lab Safety” section in the manual outlining the proper procedure of

handling hazardous materials handled during the semester.

10. Where can I find manuals to the lab equipment available to me?

The lab equipment manuals can be found in the C230 A Prep Room, located in the

drawer beneath the incubators labeled “Equipment Manuals”

11. How do I address Lab Safety in Lab?

At the start of the semester, students should be made aware of proper lab safety protocol.

You are required to show a brief video on “Lab Safety” from Flinn Scientific. Following

the video ALL students are to receive the “Lab Safety Contract” that they must read and

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sign, prior to the start of any lab. Please keep the contracts until the end of the semester,

at which time you must turn them into Jill Mitchell.

12. The lab stations are out of lab disinfectant, where can I find more?

The lab countertop disinfectant can be found in the C230 prep room to the left of the

dishwasher in a cabinet marked “Lab Disinfectant”

13. Where the emergency gas shut-off located in the lab rooms?

The emergency gas shut off is a large red button located near the door. By pushing the

button in, the gas is shut “off”.

14. What is the recharging procedure for the chargeable microscopes located in C224/

C226? Microscopes that have been used should be plugged into the outlet strip and the timer

knob turned. The timer will turn the charge off automatically after 8 hours. Microscopes

that were not used should not be plugged in during this process. Overcharging of the

scopes will shorten the life of the battery.

15. Usage of the dishwasher for glassware? What is it primarily used to clean? It can be

used to clean glassware, but it is best to have students wash glassware at the end of lab so

that glassware remains available to all labs and isn't hanging out in the dishwasher.

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Welcome to The Microbiology Lab C230

The lab and lecture rooms on campus are used frequently

for both day and evening classes. Therefore it is of utmost

importance that the materials used during class and lecture

be returned. ALL supplies and equipment should be put

away after use and all glassware needs to be cleaned.

You are permitted to prep your materials ahead of time and

place the materials on a marked cart in the prep rooms of

the lab. However following the lab sections the materials are

to be put away.

Also, it is your responsibility as an instructor to prep your

own labs. As the instructor, you are to plan accordingly to

make sure all the materials you need are available prior to

the start of lab.

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The following is a list of stocked items, and equipment available for each of the biology

courses. If you require additional supplies not present on the list, please contact Jill

Mitchell with your order ASAP to assure your purchases are made. In your order supply

the company name, item name, item number and quantity required.

Note: Not all additional orders are guaranteed and are based on funds available at the time.

BIO 110 Principles of Biology Storage Room C230B

Sugar

Glycerin

Dish detergent

Straws

pH paper

antacid tablets

3 beverages: i.e. soda, iced tea,

Gatorade, etc.

Milk

String

Pennies

Ethanol

Vegetable oil

Lemon juice

Ammonia

Benedicts solution

Iodine solution Buiret solution

Sudan IV solution

Starch solution

Glucose solution

Egg white

Elodea

Agar

Corn syrup

Syringes

Potato cores

Dialysis tubing

Well plates

Sodium bicarbonate

Food coloring

Yeast

Balloons

Bromothyml blue

Sucrose

Prepared mitosis slides

Magnetic chromosome kits

Electrophoresis gels, etc.

Evolutionary agents kit

BIO 115 Principles of Biology II Storage Room C230B

BIO 210 Microbiology Storage Room C230B

Microscopes

Staining trays

Test Tube Racks

Gloves

Prepared Slides

o Letter “e” slide

o Bacterial Shapes

o Gram Stain

Glass Slides

Cover Slips

Rubber Bulb Pippets

Toothpicks

Oil

Stains / Alcohol

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o Methylene blue

o Crystal violet

o Safranin

o Nigrosin

o Safranin

o Methyl red

o Iodine

o Ethyl alcohol

o Acid fast alcohol

o Carbol fuchsin

o Methyl green

o

Pond Water

Sterile Swabs

Bibulous paper

Kim wipes

Petri plates

Test tubes

Sterile Cotton Swabs

Inoculating Loop

Bunsen Burners

Hot Plates

Clothespins

Wax marking pencils

Alcohol Pads

Specimen (All specimen are located in

cooler unless otherwise noted)

o Escherichia coli

o Staphlococcus aureus

o Streptococcus pyogenes

o Clostridium botulinum

o Bacillus megaterium

o Corynebacterium diphtheriae

o Micrococcus luteus

o Enterobacter aerogenes

o Serretia marcescens

o Mycobacterium smegmatis

o Saccharomyces cerevisiae

What’s in your water Kit

Agar:

o Nutrient

o Eosin Methelyne Blue

o Sabouraud agar

o MacKonkey agar

o Trypticase Soy agar

o Nutrient broth

BIO 260 Anatomy and Physiology I Storage Room C226B

Microscopes

Dissecting Kits

Gloves

Synthesizing Macromolecules lab

Set

Dialysis Tubing

Iodine

Starch

Onion Root Tip / Ascaris Slides

Tissue Slides

Bones (2 boxes- green, blue)

Skeletons (4)

Muscles (see models below)

Scapels

Mammal Spinal Cord

Mammal Brain

Mammal Eye

Human Senses Lab Kit

Phenyl-thiocarbamide (PTC)

Thiourea

Sodium Benzoate

BIO 262 Anatomy and Physiology II Storage Room C230B

Microscopes

Gloves

Dissecting Kits

Blood Group Identification Lab

Mammal Heart

Double Injected Cats

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Blood Pressure Cuffs

Stethoscopes

Models:

- (2) Human Leg Muscle

- (2) Human Arm Muscule

- (2) Human Torso

- (3) Atlay Human Heart

- (3) Human Lung

- Kidney Model (Small)

- Cross Section With Description

chart

- Kidney Model (Med)

- Cross Section on stand

- Kidney Model (Large)

- Cross Section with description

booklet in base.

- Cell Model Globe

- Liver Model (Large) Cross

Section

- Reflex Arc Model (Large, Flat)

- Eye Model (small) Eyeball only

- Eye Model (Medium) in socket

with muscle and bone sites

- Eye Model (Large) in socket

with muscle and bone sites

- (5) Brain Model (small)

- external colored regions ,and

cross section marked sites

- (4) marked sites cards for

identification purposes

- Male Reproductive Organs in

box

- Brain Model (Large)

- External and Cross Section with

description booklet in base

- Brain Model in Head (Small)

LAB Equipment Prep / Storage Room C230A/B

- Microscopes

- Balances

- Hot Plates

- Stirrers

- Water Distiller

- Bunsen Burners

- Microscopes

- Autoclave

- Incubators (2)

- Fume Hood (2)

- Dishwasher

- Cooler

- Refrigerator

- Gel Electrophoresis Chambers

- Micropippeters

- Thermometers

- Glassware

- Test Tube Racks

Note: The lab equipment manuals can be found in the C230 A Prep Room in a

cabinet drawer labeled, “Equipment Manuals”.

LAB Equipment C226 Prep / Storage Room C226A/B

- Beakers

- Microscopes

- Yard Sticks

- Cooler

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Index

A. OSHA Laboratory Safety Guidance

B. Disposal of Biological Specimen

C. OSHA Bloodborne Pathogens and Hazard Communication Standards

A. OSHA Laboratory Safety Guidance

Here are a list of helpful links to documents provided by the Occupational Safety and Health

Administration.

Laboratory Safety Guidance

OSHA Fact Sheet OSHA’s Bloodborne Pathogens Standard

OSHA Fact Sheet Chemical Hygiene Plan

OSHA Fact Sheet Laboratory Safety OSHA Laboratory Standard

OSHA Quick Facts Laboratory Safety Autoclaves / Sterilizers

OSHA Quick Facts Laboratory Safety Labeling and transferring of Chemicals

Other Helpful Links:

Material Safety Data Sheets

Center for Disease Control and BIO Safety

World Health Organization

Management and Disposal of Biological Waste

At Pennsylvania Highlands Community College

I. Introduction

The purpose of this document is to provide information, requirements, guidelines and

procedures for the handling and disposal of hazardous and non-hazardous biological

waste for all departments and units on the Pennsylvania Highlands Community

Pennsylvania Highlands Community College

College campus. Currently there are three departments or units that generate

biowaste: Science, Healthcare Management, and Workforce Education.

“Biological Waste” or “Biowaste” means discarded biological material from teaching

and clinical laboratories and operations. This does not include household or office

trash.

“Biohazardous Waste” means any solid or liquid biological waste that is hazardous

because of its physical and/or biological nature. All waste that contains infectious

material, or because of its biological nature, may be harmful to humans, animals,

plants, or the environment is biohazardous waste. This includes waste from

infectious animals, human blood or blood products, pathological waste, and sharps.

II. Responsibility

Faculty member or other person with operational responsibility

1. Shall be responsible for ensuring students in classes generating biohazard waste

comply with the provisions of the plan.

2. Shall take appropriate steps to assure the safety of the students involved in the

handling of biowaste.

3. Will take immediate decontamination measures, primarily consisting of intensive

flushing, and other preliminary first aid procedures in the event of a spill or other

accident, while notifying the Director of Security and Safety or his employees

who will in turn notify appropriate medical and HAZMAT first responders.

Science lab manager, Health Care Management appointee, Director of

Workforce Education

1. Shall be responsible for the proper storage, handling and packaging for disposal

of biological waste generated from their respective programs. Material for

disposal shall be clearly marked in the red biohazard bags provided, and stored in

the “prep” rooms, room 224A for Healthcare Mgmt. and Workforce Ed., as well

as in room 230 for Science, pending pickup. Boxes should also be clearly marked

with the name of the originating department for billing purposes. Access to these

locked rooms shall be strictly limited to appropriate faculty, or to students under

direct supervision of those faculty members, and all access doors will be clearly

marked with signs depicting the universal biohazard symbol.

2. Shall notify the Director of Security and Safety when a sufficient number of

containers of biowaste or sharps are ready for disposal (2-3 standard boxes) so

that a pickup can be scheduled. Will also place orders for replacement containers

at this time.

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3. Shall immediately report spills or other events to the Office of Security and

Safety, while ensuring that any necessary decontamination and first aid to affected

persons is immediately performed. The Office of Security will respond

immediately to the scene of the incident and will coordinate immediate

deployment of medical and HAZMAT first responders as appropriate.

Facilities Manager

1. Shall be responsible for receiving requested replacement biowaste supplies from

the disposal company at the time of a scheduled pick-up and distributing the same

to the various departments.

2. Shall be responsible for directing invoices and other paperwork resulting from a

pickup to the Director of Security and Safety, who will in turn file or distribute to

the appropriate departments as necessary.

3. Shall be responsible for transporting containers of biowaste from the “prep” room

to a holding area. This includes assuring that the containers are secure while they

await removal by the disposal company. Containers of “sharps” will be

transferred to the holding area approximately one hour prior to the anticipated

arrival of the disposal company.

4. Facilities Manager will oversee the actual loading and removal of biowaste.

Director of Security and Safety

1. Shall assume primary oversight responsibility for procedures as outlined in this

plan, as well as ensuring that they conform to applicable state and federal

regulations.

2. Shall be notified immediately of any incident occurring on PHCC property

involving biowaste spills or other related accidents, particularly those which may

result in the contamination of students, faculty, staff or others. Will coordinate

notification to appropriate medical and HAZMAT first responders, and will

proceed immediately to the accident scene to assist with decontamination and first

aid, as well as the evacuation of personnel and securing the area. Subsequent

notification of the incident will also be made to the PA Department of

Environmental Protection.

3. Following the incident will complete a PHCC Incident/Injury report, and will

convene an after-action meeting attended by all involved parties for the purpose

of identifying the cause(s) of the accident, as well as making recommendations

aimed at avoiding a reoccurrence.

4. Will ensure that all required records and notifications to regulatory agencies are

being accomplished correctly and in a timely fashion. Will maintain a file of

Department of Environmental Protection (DEP) forms # 2540, Infectious and

Chemotherapeutic Manifest, documenting pickup and eventual disposal (i.e. chain

of custody).

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5. Upon notification by the referenced departments that a pickup is required, shall

contact the disposal company and make arrangements, and that information will

be relayed to the departments and to the Facilities Manager. Will also relay

orders for replacement biowaste containers as requested from the departments.

III. Segregation

1. Any waste that could produce lacerations or puncture injuries must be disposed

of as “sharps”. Sharps must be segregated from other waste. Metal sharps and

broken glass that pose a biological hazard may be comingled with each other, but

not with other waste. Sharps should not be comingled with regular laboratory

glassware which has been broken and disposed of in an appropriate and separate

container.

2. Biological waste must not be comingled with chemical waste or other laboratory

trash.

3. Biohazardous waste should be separated from other biological waste.

IV. Containers

Containers must be appropriate for the contents; not leak; be properly labeled; and

maintain their integrity if thermal or chemical treatment is used. Containers of

biohazardous materials must be kept closed.

1. Sharps—use a rigid, puncture-resistant container (heavy-walled plastic is

recommended) for storage and disposal. These are usually provided by the

disposal company.

2. Solid Biohazardous Waste—Use heavy- duty plastic “Biohazard Bags” (autoclave

bags) or containers for biohazardous waste.

3. Solid Non-hazardous Waste—Use heavy-duty plastic bag or other appropriate

container without a Biohazard symbol.

V. Storage

Biological waste may be held temporarily under refrigeration, prior to disposal, in a

safe manner that does not create aesthetic problems. Biohazardous waste should be

treated and disposed of promptly. Containers holding biohazardous material must be

clearly labeled, including the biohazard symbol.

Storage of any type of biological waste, as well as any other disposal materials, for

the various departments are as follows:

Science—the storage or prep room adjacent to Rm C230

Healthcare Management—the smaller storage room off of the large prep room

between Rms. C224 and C226.

Workforce Education—the large prep room between Rms. C224 and C226.

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VI. Procedures for Treatment and Disposal

1. Microbiological Waste

a.) Should be treated (disinfected) promptly on-site by autoclaving at 15 psi and

at least 121 °C for at least 30 minutes. Autoclaving is the preferred

disinfection method. However, if autoclaving is not possible, microbiological

waste may be chemically disinfected by immersion, for not less than three

minutes, in a 1:10 (volume/volume) bleach solution.

b.) Once treated as above, the waste may be deposited in the regular trash as long

as the autoclave bag has been labeled “treated” and then placed in another bag

that is opaque—but not a biohazard bag.

2. Animal Carcasses and Body Parts

The dissection specimens that the college purchases may be disposed of

by way of the regular trash according to the manufacturer. However, it is current

policy to dispose of preserved specimens via a disposal company. Therefore,

these carcasses and body parts should be handled as follows:

a.) During the semester or session, the specimens should be double-bagged, to

prevent leakage, and stored in the refrigerator nearest the lab where the

dissection is being performed.

b.) At the end of the semester, the double-bagged specimens should be placed

into a cardboard container, supplied by the disposal company, that is lined

with a red biowaste bag. Care should be exercised to not exceed the weight

limit of the box, which is typically 50 lbs. In all cases the packaging

instructions of the disposal company should be followed (See attached).

c.) Notify the Facilities Manager when the package is ready for disposal.

3. Sharps

a.) Sharps should be placed into an approved sharps container immediately after

use. The container should be kept closed between uses.

b.) At the end of the semester or when the sharps container is full, the container

should be placed into a cardboard container, supplied by the disposal

company, that is lined with a red biowaste bag. In all cases the packaging

instructions of the disposal company should be followed (See attached).

c.) Notify the Director of Security and Safety when the package is ready for

disposal.

WEW/9-25-2009

Pennsylvania Highlands Community College