FOLLICULAR DYNAMICS AND UTERINE STATUS AFTER SYNCHRONIZATION

OF OVULATION IN EARLY POST-PARTURIENT BUFFALO COWS

Sosa, G.A.; Kandiel, M.M. ; Gad, B.A. and El-Azab, A.I. . . . . u .

- . u aimed at i ti ati t e u a a ia a d ute i e e e t

t e i itiated da t a tum . ima e e a a ed i t t eated a d t u . e t eated u a i e st d se da da ed a a da a d nd d se n n da . anima s e e

a ed a dai u t as und e aminati n m da - t da and t en a te e e s unti dete ti n u ati n. T e a ian es nse t e t eated u ea s ed t e

ani ed e ents i u a t ended u ati n in ne anima . t e t eated u t e an es in t e sma medium a e and t ta i e u ati n and a ea did n t di e si ni i ant et een t e t u s uteini ati n t e d minant i e

in t e i st n in e ti n in t anima s . indi ated t e e ia i it s n st ate t im e t e a ian un ti n t e ute ine es nse as ea e iden ed in easin t e ute ine a m eneit de easin t e ute ine umen ess t an . m

and ea in ntents da - 8 in the treated r u . n n usi n the s n h r t has a ene i ia e e t n the arian un ti n and uterine in uti n hen a ied da s in bu a es. e rd u a es ar s n h st artum tras und.

INTRODUCTION ntr the inter a r m arturiti n t subse uent n e ti n is im rtant t

timi e the re r du ti e rate a s e ies. u a es are hara teri ed b their re r du ti e e i ien as e ressed b n a in inter a e eedin da s ert et a m st due t the ered arian a ti it - ish . n enera the

arian res nse bu a es t stimu at r treatment seemed t be ess than that re rted in att e T te et a . in h et a . n bu a es the ered a ai abi it an u at r i e resu ts in r du in e and r ua it embr s

adan et a . and isra et a 88 . n dair s the st artum an u at r anestrus in dair s as attributed n t

n t a a i u ar de e ment but a s t the ai ure a d minant i e t u ate he et a . usa e n in a sin e in e ti n auses an in rease in the LH

sur e and u ati n durin st artum bet een da s – 8 in dair ha enber er et a . 8 and da s - in beef Tr e and es er 8 att e. three in e ti n s hedu e n H- - n H named s n h as su essfu im i ated f r s n hr ni ati n f u ati n in att e urs e et a and buffa es au and ra ash .

n the mean time f a r in that the s n h r t effe ti e indu es u ati n in dair s as ear as da s st artum ma a- nt a et a there

is n a ai ab e inf rmati n n its usa e durin an ear st artum sta e in buffa es. The resent stud as desi ned t e a uate the arian res nse and uterine han es f in

s n hr ni ati n f u ati n b s n h r t a ied ear ier after a in as a int f

165

Lu r ri tiin ifi e – seria edi in eterinar

e n mi a ue in r m tin the r du ti e and re r du ti e tentia s f buffa es.. Materials and Methods The resent stud as arried ut n a t ta number f si ne arturient a tatin buffa s e t in the du ati na arm a u t f eterinar edi ine enha ni . durin the eri d fr m ri t u ust . anima s ere h used in a free-sta

barn ffered f mi ed rati n f r a tati n nsisted f tt n seed a ine seed a e rn bran m asses ime and a n e dai and su ed t i e dai

and 8 h and the a era e mi ie d as a r imate - da . anima s ere e amined u tras n ra hi a bef re the start f treatment f r identif in the state f u ati n and resen e f r us uteum n the ar . nima s

ere rand m a ated in near t simi ar r u s f r treatment and ntr ea h f hi h as buffa s.

nima s in the treated r u n ere treated ith the s n h r t i ure a rdin t urs e et a . a anima s re ei ed i .m. in e ti n f usere in a etate as n H ana ue e e ta nter et nternati na . . e ua m n

da da f ed b i.m. in e ti n f s ntheti r sten strumate herin - u h nima hea th e ua m n da then b se nd i.m.

in e ti n f n H ana ue n da . n the ntr r u a anima s ere in e ted b sa ine m arab e t the d se and time f the h rm na treatment. usin transre ta u tras n ra h ie edi a -8 H inear arra r be

arian m r h as m nit red dai startin fr m the da bef re the first t the se nd GnRH in e ti n then e after t i e dai 8. and . h ti urren e f

u ati n and re-e amined after da s t nfirm u ati n and urren e f the CL. T ana e the han es in f i u ar d nami s after GnRH treatment a antra f i es mm

ere unted and measured. The bser ed f i es ere assified int sma . m medium . - . m and ar e . m si es . iameters and umes m f utea stru tures and a ities ere determined. The trans erse diameter f the anteri r se ti n f b th uterine h rns and the r ressi n f uterine in uti n ere e a uated.

nima s ere bser ed t i e dai f r at east min bef re mi in b e erien ed ers n t dete t si ns f estrus and t be bred natura i ure .

The btained data as tabu ated and statisti a m uted here a r riate b the inear re ressi n ana sis usin a i r s ft e m uter r ram a rdin t

asthi et a . Results: 1- Ovarian findings 1.1-. Follicular dynamics s sh n in fi ures and the han es in the number and area f sma medium and ar e f i u ar si e . m . - . m and . m diameter res e ti e and h e f i u ar u ati n in the treated and ntr r u s did n t sh an si nifi ant ariati n a n the e eriment eri d. re er the hara teristi s f f i u ar

a es tab e sh ed a simi ar attern in the t r u s e e t f r the diameter f the d minant f i e at st GnRH treatment hi h as si nifi ant ar er ri r t uteini ati n

. 8 . s . 8 . m . . 1.2- Ovulatory response s sh n fr m fi ures & 8 there as an urren e f uteini ati n f the d minant f i e L after first GnRH in e ti n in ut f treated anima s . and

166

Universitatea de tiin e ri e i edi in eterinar a i

fai ure f vu ati n r CL deve ment. uteini ati n f the ar est f i e as evident u tras n ra hi a in res nded buffa es b thi enin in the a . m idth and in reasin the e h eni it f the f i u ar a ne da after first GnRH treatment rea hed its ma imum diameter . m n da re ressi n f the utea stru ture started b de reasin the e h eni it the diameter and a sin the avit area fr m da and be ame u tras n ra hi a diffi u t t be dete tab e b da - bef re G treatment. ut f three treated anima s there as ne buffa sh ed . and

vu ati n - hrs after the se nd GnRH in e ti n. 2- Uterine findings:

The d rsa uterine diameter i . did n t differ bet een the treated - . . R . and ntr - . . R .8 r u s e e t n da here it

sh ed a si nifi ant de rease . in the treated r u . The ventra diameter f the uterus i . sh ed a si nifi ant . de rease n da and f ed b a si nifi ant . in rease n da in the treated r u b th r u s ere ne ative

rre ated ith da s st-treatment - . . R . 8 and - 8 . 8 . R .8 res e t. . the rania diameter i . C sh ed a si nifi ant in rease n da

8 and b th treated and ntr r u s ere ne ative rre ated ith da s st-treatment - . . R .8 and - . .88 R . res e t. . The transverse diameter in the treated r u i . sh ed si nifi ant . de rease

n da and but si nifi ant . in rease n da hen m ared t that in the ntr r u - . . 8 R . and - . . R .8 res e t . The uterine umen i . revea ed hi h si nifi ant . de rease a n the da s f the e eriment in the treated r u hen m ared ith ntr - . . R

.8 and - . . R . res e t.

Discussion The resent stud revea ed that the vs n h r t a ied at an ear

st artum eri d da re ise s n hr ni ed vu ati n ithin - h after the se nd-GnRH treatment in . buffa es and indu ed ear earan e f uterine se reti n as eviden ed b redu in the uterine umen diameter. H ever the han es in the t ta f i u ar u ati n and r area ere n t si nifi ant different bet een the treated and the ntr r u s. The r varian res nse in the treated r u t the first GnRH basi a mi ht be attributed either t the itt e u ati n f H-de endant f i es the LH se reti n fr m ituitar in res nse t GnRH in e ti n r the GnRH re e t rs in ituitar and . This findin ame in ass iati n ith s me revi us re rts uf ur and R 8 Lu et a . urs e et a . a ters et a 8 indi atin that the hi h rates f vu ati n after GnRH in e ti n are due t the resen e f

tentia vu at r f i e . mm the ear st artum eri d is hara teri ed b redu ti n in the number f sma si ed f i es as the number f da s st artum in reases Lu et a . - ish .

The present resu ts revea ed uteini ati n f the d minant f i e f in the first GnRH in e ti n in the treated r up a findin hi h emphasi ed urren e f

vu ati n in resp nse t GnRH treatment durin the p stpartum peri d f r buffa es aruse i et a . and 8 att e i tban . i e uteini ati n as dete ted

in . n n- i buffa es s n hr ni ed b vs n h - 8 da s p stpartum i and ahm .

167

Lu r ri tiin ifi e – v seria edi in eterinar

The present stud indi ated urren e f vu ati n in ne ase f the treated r up . - h after the se nd GnRH in e ti n. This findin ame in nsistent ith

that rep rted ear ier in s em ra et a and buffa es arria h et a . 8 assumin that vs n h pr t f r estrus s n hr ni ati n has p tentia app i ati n f r impr vement f ferti it in anestr us buffa es durin ear p stpartum peri d even durin e treme summer m nths thr u h suppressi n f pr a tin se reti n R and

ra ash . in GnRH in e ti n intensit f estrus as dete ted a findin hi h ame in a rdan e ith s me previ us studies attabiraman et a . 8 ar a i et a .

indi atin the presen e f p r si ns f heat in GnRH treated buffa es. The present stud sh ed f u tuati n in thi ness f the uterus but as ne ative rre ated ith the da p stpartum. The treated r up sh ed a pr minant

earan e f the uterine umen as indi ated fr m the n ti eab e de rease f the uterine umen e h eni it and diameter mparab e t the ntr ne. This mi ht be attributed

t the in reased varian estradi se reti n b ar e f i e s that has a a effe t t in rease the rate f uterine inv uti n he d n and bs n he d n et a . The u tras n raphi pattern f de reasin the uterine diameter b in reasin time after parturiti n ame in a rdan e ith s me previ us studies an and T mi u a 8 Tian and a es amimura et a he d n et a . esides the transverse uterine diameter re rded in the present stud f r the treated and ntr r ups ame in in nsistent ith previ us rep rts in buffa es Usmani et a . L han et a

hasati a et a . r m the present stud it an be n uded that the vs n h pr t is

p tentia ab e t impr ve the varian and uterine fun ti n hen app ied durin ear p stpartum in buffa es a findin hi h needs t be nfirmed in further stud n a bi er number f p stparturent buffa s. References:

. i ahm S. varian d nami s and mi pr ester ne n entrati ns in in and n n- in buffa - s uba us buba is durin vs n h pr ram.

Theri en 8 - 8 . ma a- nt a C atsui a ashima C Ha ashi G atsuda G ane ida

i am t i a e ndu ti n f vu ati n ith GnRH and G a pha at t different sta es durin the ear p stpartum peri d in dair s varian resp nse and han es in h rm ne n entrati ns. Repr d ev. 8 -8 .

. asthi hare bhishe avani S Siddi uee G pan ha T shah RR is ne- ave f i u ar r th durin the estr us e a usua phen men n in ater buffa es buba us buba is . nima Repr du ti n S ien e - .

. ar a i H ar ha H - rad ffe t f treatment ith GnRH ana ue n p stpartum repr du tive perf rman e f su in ptian buffa s. uffa

- . aruse i . S. . H. adureira . H. anarbe R. C. arnarbe and R. Cassimir .

va uati n f s n hr ni ati n f vu ati n f r fi ed time inseminati n. ra i ian . et. Res. nim. S i. - .

. em ra ـ ba e re T ض t r . The ffe tiveness f C s n h r t in air Heifers and u tipar us C s. Tur et nim S i -

168

Universitatea de tiin e ri e i edi in eterinar a i

. uf ur and R GL 8 . istributi n f varian f i u ar p pu ati ns in the dair ithin da s after parturiti n. . Repr d. erti . .

8. - ish The p stpartum buffa . nd rin i a han es and uterine inv uti n. nim Repr d S i. - .

. amimura S ha i T Ta ahashi Tsu am t T stpartum resumpti n f varian a tivit and uterine inv uti n m nit red b u tras raph in H stein s. et ed S i -

. hasati a CT avani S hami erashri H an ha T esai studies n puerpera events and repr du tive effi ien f in h rm ne therap at da p stpartum in surti buffa es internati na urna f a ri u ture & bi 8 -

. L han S a i R a er L Uterine inv uti n and varian f i u ar r th durin ear p stpartum peri d f urrah buffa es uba us buba is . sian-

ustra asian urna f nima S ien es . Lu C Stap es CR i he That her t ffe t f feedin a ium

s aps t ear p stpartum dair s n p asma pr sta andin a pha uteini in h rm ne and f i u ar r th. . air S i. 8 .

. adan .L. S. . as and . a ta. . pp i ati n f repr du tive te hn t buffa . nim. Repr d. S i. - .

. etr G.H. i . . e R. . -Ri a at H. . . a t rs affe tin the perf rman e f ptian buffa . ri . S i. sht h r - 8 .

. isra . . .C. adav and .T. t ani. 88. Su essfu embr transfer in a buffa uba us buba is . n r . f the Se nd r d uffa C n ress e e hi ndia .

. an and T mi u a T 8 U tras ni bservati n f p stpartum uterine inv uti n in the . Theri en -

. attabiraman SR eerapandian C ua am S 8 ffe ts f Re epta treatment in an estrus and ear p stpartum s and buffa es. ndian et -

8. au ra ash S. ffi a f the vs n h pr t f r s n hr ni ati n f vu ati n and fi ed-time artifi ia inseminati n in urrah buffa es uba us

buba is .Theri en . - . . urs e R ee i tban C S n hr ni ati n f vu ati n in dair s

usin G a pha and GnRH. Theri en - . . R he a e is in . Repr du tive mana ement f p stpartum s.

nim Repr d S i. - - Revie . . R S and ra ash S . Chan es in end rine pr fi es durin vs n h and

vs n h p us n rpr a treatment in urrah buffa heifers at h t summer seas n. Tr pi a nima Hea th and r du ti n - 8

. S ha enber er S hams u ermann a ters L. 8 u sati e se reti n f nad tr phins varian ster ids and varian t in durin pr sta andin-indu ed

re ressi n f the rpus uteum in the . Repr d erti . - . . She d n bs n H . ffe t f administrati n f eCG t p stpartum s n

f i u enesis in the var ipsi atera t the previ us ravid uterine h rn and uterine inv uti n. Repr d ert -

. She d n a es R r ft bs n H . The effe t f intrauterine administrati n f estradi n p stpartum uterine inv uti n in att e. Theri en

-

169

Lu r ri tiin ifi e – v seria edi in eterinar

. Sin h anda S & dams G The repr du tive pattern and effi ien f fema e buffa es. nima Repr du ti n S ien e -

. Tian and a es ffe t f f ur h rm ne treatments after a vin n uterine and ervi a inv uti n and varian a tivit in s. et Re 8 - .

. T te S. . G. Sin h . Tane a and G. . Ta ar. . n vitr maturati n and ferti i ati n f f i u ar tes fr m buffa .Theri en 8 .

8. Tr e TR es er . 8 The effe t f pr estin and GnRH treatments n varian fun ti n and repr du tive h rm ne se reti ns f anestr us p stpartum su ed beef

s.Theri en . - . . Usmani RH hmad Shafi ir a ffe t f sub ini a uterine infe ti n

n ervi a and uterine inv uti n estr us a tivit and ferti it in p stpartum buffa es. Theri en - .

. a ters H ai e TL ears n R G a daus asn C 8 arit -Re ated Chan es in vine i e and te pu ati ns te ua it and H rm nes t a s stpartum. air S i 8 8 -8 .

. arria h H Channa hmad 8. ffe t f estrus s n hr ni ati n meth ds n estrus behavi ur timin f vu ati n and pre nan rate durin the breedin and breedin seas ns in i i-Ravi buffa es. nim Repr d S i. -

. i tban . C. . H inf rmati n n h rm na re u ati n f the var has impr ved understandin f the timed breedin pr rams. n r eed. nnua

eetin f S iet f r Theri en nterea Canada pp 8 - .

170

Universitatea de tiin e ri e i edi in eterinar a i

Figure (1): ia rammati s heme f vs n h re imen and pr t f r . US u tras und e aminati n p stpartum GnRH nad tr phin re easin h rm ne G pr sta andin

Figure (2): U tras n raphi measurin f the d rsa rania and ventra diameters f a uterine h rn

171

Lu r ri tiin ifi e – v seria edi in eterinar

Figure (3): Chan es in the hara teristi s f sma f i e p pu ati n . m in the buffa es treated and ntr r ups f in vs n h GnRH- G -GnRH ) pr t started n da da s p stpartum . th f i u ar number ) (and area ) (did n t differ si nifi ant bet een the t r ups a n the m nit red peri d . a ues presented ere means.

172

Universitatea de tiin e ri e i edi in eterinar a i

Figure (4): Chan es in the hara teristi s f medium f i e p pu ati n . - . m) in the buffa es treated and ntr r ups f in vs n h )GnRH- G -GnRH (pr t started n da da s p stpartum . th f i u ar number ) (and area ) (did n t differ si nifi ant bet een the t r ups a n the m nit red peri d . a ues presented ere means.

173

Lu r ri tiin ifi e – v seria edi in eterinar

Figure (5): Chan es in the hara teristi s f ar e f i e p pu ati n . m in the buffa es treated and ntr r ups f in vs n h GnRH- G -GnRH pr t started n da da s p stpartum . th f i u ar number and area did n t differ si nifi ant bet een the t r ups a n the m nit red peri d . a ues presented ere means

174

Universitatea de tiin e ri e i edi in eterinar a i

Figure (6): Chan es in the hara teristi s f t ta f i e p pu ati n . m in the buffa es treated and ntr r ups f in vs n h GnRH- G -GnRH pr t started n da da s p stpartum . th f i u ar number and area did n t differ si nifi ant bet een the t r ups a n the m nit red peri d . a ues presented ere means.

175

Lu r ri tiin ifi e – v seria edi in eterinar

Figure (7): Representative patterns f f i u ar r th and re ressi n n the var f buffa es treated ith vs n h pr t da s p stpartum .The ar est f i e in the treated r up sh ed a uni ue pattern f r th that ended b vu ati n in ne buffa

hi e re ressed in the ther t anima s . tted and dashed ines refer t timin f GnRH and G treatment respe tive .Lut : uteini ati n v : vu ati n.

176

Universitatea de tiin e ri e i edi in eterinar a i

Figure (8) The pattern f indu e utea tissue deve pment and re ressi n n the var f 8treated buffa es ith vs n h pr t da s p stpartum. The uteini ati n f d minant f i e started n a .The utea tissue v ume sh ed a rapid de rease per unit f time as mpared ith utea tissue diameter

177

Lu r ri tiin ifi e – v seria edi in eterinar

Table 7: Chara teristi s ean S. . f the varian aves in ptian uffa s treated ith vs n h durin ear p stpartum peri d parameters

Treated r up C ntr r up P-va ue ean S ean S -test t-test

iameter f d minant at start f treatment m

. 8a . . 8b . . .

a .f i e diameter after treatment m

. 8 . 8 . . . .

Gr th rate f the d minant f i e mm/d . . . . . .

Len th f r th phase da s 8. . . . . . Ran e – da s – da s

a f emer en e f the d minant f i e

. . . . . .

Ran e – da s – da s e innin diameter m . . . . . .8 a f ma imum diam. f the

d minant f i e . . . . . .

Ran e – da s – da s a f emer en e f the st ave after treatment

. . . .88 . .

Ran e – da s – da s Linear r th rate f the d minant f i e

. . . . . .

ma diameter f nd ar est f i e

. . .88 . . .

a f deviati n . . . . . . Ran e – da s – da s

178

Universitatea de tiin e ri e i edi in eterinar a i

Figure ( 9) representative u tras n raphi pi tures f the uterus n a in the treated ri ht side pane and ntr eft side pane buffa es ith vs n h pr t started n a p stpartum. The h m en it f the uterine a in reased hi e the umen diameter and e h eni it de reased in the treated r up mpared ith ntr .

The arr refers t the dete tab e uterine umen in the ntr r up n a .

179

Lu r ri tiin ifi e – v seria edi in eterinar

Figure (10): Chan es in the uterine d rsa rania and ventra diameter f the uterine a in the treated and ntr r ups treated ith vs n h pr t started n

a p stpartum. The rania and ventra diameter f uterine h rn s in reased si nifi ant in the treated r up due t the in rease in vas u arit a mpan in the nset

f estrus . a ues presented are mean S * .P . **P . .

180

Universitatea de tiin e ri e i edi in eterinar a i

Figure (11) Chan es in the transverse uterine diameter and umen in the treated and ntr r ups treated ith vs n h pr t started n a p stpartum. f the diameter ta en as a uide t nfirm the uterine inv uti n m it did n t mp ete neither in treated n r in ntr r ups . f the umen diameter used as a uide . m the uterus f treated anima s inv uted b a i.e . 8 da s PP . The uterine umen sh ed a pr ressive and si nifi ant de rease in the treated anima s mpared ith the

ntr r up. a ues presented are mean S * .P . **P . .

181