ABSTRACT

ABSTRACT

DETECTION OF ENTEROPATHOGENIC Escherichia coli (EPEC) O86, O114, O125, O127, O128 FROM CHILDREN UNDER-FIVE YEARS WITH DIARRHEA ATTENDING THE HOSPITALITY PRIMARY HEALTH CARE IN PEKANBARU

By

SABELLA GUSTIKA VERNANDA

Diarrheal diseases remain one of the leading cause of childhood morbidity and mortality in most developing countries, with Enteropathogenic Escherichia coli (EPEC) being one of the most important aetiologic agents of children under-five years diarrhea in many of these countries. In Indonesia especially Pekanbaru, the epidemiology of these EPEC have not been well studied. During the period from April 2014 to September 2014, 47 stool sample were collected from children under-five years with acute diarrhea in the hospitality primary health care in Pekanbaru and the examination has been done in Microbiology Laboratory of Medical Faculty Riau University. The Escherichia coli were isolated and diagnosed by standard microbiological methods. The isolates confirmed to be Esherichia coli were subjected to a slide agglutination test with Escherichia coli polyvalent antisera. The following typing sera was used polyvalent 3 (O86, O114, O125, O127, O128). This study was a descriptive study using a consecutive sampling method to describe the prevalence of Enteropathogenic Escherichia coli (EPEC) O86, O114, O125, O127, O128. Based on gender and age from all specimens that were collected, the diarrhea mostly found in male (59.5%), and in 12-59 months patients (93.6%). Most of the patients who had diarrhea experiencing fever (57.4%) and mild-moderate dehydration (74.5%). Out of the 47 stool samples, 1 isolate (2.2%) were positive for EPEC O86, O114, O125, O127, O128. The isolate was recovered from the 19 months female who experienced fever and mild-moderate dehydration.

Key words: diarrhea, enteropathogenic Escherichia coli, EPEC, children under-five years, hospitality primary health care

ABSTRAK

PEMERIKSAAN ENTEROPATHOGENIC Escherichia coli (EPEC) O86, O114, O125, O127, O128 PADA BALITA DENGAN DIARE DI PUSKESMAS RAWAT INAP KOTA PEKANBARU

Oleh

SABELLA GUSTIKA VERNANDA

Diare merupakan penyebab utama morbiditas dan mortalitas pada balita dibanyak negara berkembang, dengan Enteropathogenic Echerichia coli (EPEC) sebagai salah satu etiologi terpenting pada balita dengan diare di banyak negara berkembang tersebut. Di Indonesia, khususnya Pekanbaru, epidemiologi dari EPEC masih belum diteliti secara mendalam. Selama kurun waktu April 2014 sampai dengan September 2014, berhasil dikumpulkan sampel tinja sebanyak 47 sampel dari balita dengan diare akut di Puskesmas Rawat Inap Kota Pekanbaru. Pemeriksaan kultur tinja dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Riau. Escherichia coli diidentifikasi dan diisolasi menggunakan metode mikrobiologi standar. Isolat yang positif Escherichia coli kemudian di uji kembali menggunakan tes slide aglutinasi dengan antiserum polyvalent Escherichia coli. Polyvalent yang digunakan terdiri dari O86, O114, O125, O127, O128. Penelitian ini merupakan penelitian deskriptif dengan metode consecutive sampling untuk mengetahui gambaran prevalensi dari EPEC O86, O114, O125, O127, O128. Berdasarkan umur dan jenis kelamin dari seluruh 47 sampel yang berhasil dikumpulkan, diare terbanyak ditemukan pada balita laki-laki (59,5%) dan dalam rentang usia 12-59 bulan (93,6%). Mayoritas pasien mengalami demam (57,4%) dan dehidrasi ringan-sedang (74,5%). Dari seluruh 47 sampel, didapatkan 1 isolat (2,2%) yang positif EPEC O86, O114, O125, O127, O128. Isolat ditemukan pada tinja balita perempuan yang mengalami demam dan dehidrasi ringan-sedang.

Kata kunci: diare, enteropathogenic Escherichia coli, EPEC, balita, puskesmas rawat inapv

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