EliminatEs troublEs with bubblEs - Biotech · 2020. 9. 17. · debubblers - contact us! no troublEs...

1

EliminatEs troublEs with bubblEs

2

what is thE fundamEntal diffErEncE bEtwEEn thE dEgassEr and dEbubblEr?

the degasser removes gasses

dissolved in the mobile phase/flow

systems. the debubbler catches and

removes bubbles visible to your eyes.

for further information regarding

debubblers - contact us!

no troublEs with bubblEs

anymorE

Dissolved gasses in a fluidic system can often cause troubles. When the pressure or the temperature changes, the dissolved gasses can form bubbles which affect the accuracy, precision and perfor-mance of your equipment. On-line degassing is a very efficient way of removing dissolved gasses from the liquid and preventing bubble formation.

biotEch dEgasi sEriEs arE dEsignEd to rEmovE dissolvEd gassEs from a solvEnt. The degassers can be employed in all type of applications where gasses dissolved in the operating liquid may interfere with the use of the system. Degassing is an absolute requirement to get optimal performance out of your analytical instrumentation or chromatography system! The use of a BIOTECH DEGASi system will save you time and money by avoiding interruptions in your production.

The BIOTECH DEGASi line is easy to use and provides reliable con-tinuous operation for many years. The extremely low internal volume of the Systec AF™ tubing used in the degasser provides for quick equilibration and very short startup times, compared to the use of a degasser which uses PTFE® degassing channels with the same degassing efficiency. The product range includes six different degassers where you select a model depending on the flow rate and what type of solvent used.

– prevent bubble formation in your fluidic system with BIOTECH DEGASi line of degassers.

lEt thE flow volumE guidE thE choicE of dEgasi

3

analytical instrumEntation dEgassingget the best performance out of your instrumentation. wide range of application areas.• chromatography - hplc/uhplc - gpc- preparative• biosensor applications• biotechnology

DEGASSING applications:

a biotEch DEGASi for EvEry application

classic

gEnEralpurposE

gpc

gpcEluEnt

micro

limitEdvolumE

sEmi-prEp

sEmiprEp

prEp

hi-EndprEp

compactapplication

compact

systec af™ internal volume

480 µl 480 µl 100 µl 925 µl 8.4 ml / 13.8 ml 285 µl

no of channels 2-5 2-5 2-5 2-5 2 2, 4, 6

biocompatible flow path

y n y y y y

approximately max flow per channel (ml/min)

3 3 0.5 6 25 / 50 2

inner dimension flow path (mm)

1.14 1.14 1.14 1.14 1.91 0.89

4

gEt rid of dissolvEd gassEs with our DEGASi-linE

dEgassing ExplainEdThe critical component of the vacuum degasser is a short length of Systec AF™ tubing through which the solvent flows. This tubing is located in a chamber where a partial vacuum is maintained by a vacuum pump that is constant-ly running at a low speed. Dissolved gasses migrate across the tubing wall under a concentration gradientproduced by the vacuum as the solvent flows within the tubing in accordance with Henry’s law. The gasses are expelled from the system and the chamber is maintainedat a constant, preset vacuum level by varying the vacuum

pump speed as needed. A special port in the vacuum pump continually flushes the pump head with a small “bleed” of air to remove any solvent vapors which may enter the pump from the vacuum chamber. This air bleed eliminates the need for any solenoid valves within the system. This patented design results in zero vacuum “hysteresis”.

It is not necessary to totally eliminate the dissolved gas, it only needs to be reduced to a concentration that is below the saturation point of the mixture. Typically, ~50% must be removed.

thE principlE of our dEgasi-linE

Zhcr®vacuum pump

dissolved gas

systec af™

Eluent

vacuum controller

injector /auto sampler

dEgassingchambEr

fluidicpump

dissolved gasses are actively removed from a flowing liquid stream by vacuum via the systec af™ membrane.

5

classicmicro sEmi-prEp

thE dEgassing chambEr thE hEart of our DEGASi-linE

thE sEcrEt rEvEalEdTh e Systec AF™ membrane is an essential part of the degassing process. Systec AF™consists of an amorphous perfl uorinated copolymer. Th rough the highly permeable membrane the dissolved gasses are removed, by applying vacuum on the outside of the membrane, while the liquid stays on the inside.

Th e fl ow path is inert and most of our diff erent degas-sing chambers have a biocompatible fl ow path. Every vacu-um chamber is manufactured with the highest quality and tested individually to ensure top-of-the-line performance.

Depending on your needs regarding conditions such as type of solvents and fl ow rates, we can help you determine the size of the vacuum chamber that will be optimal for your application.

6

incrEasE thE Quality using systEc Zhcr®

and systEc af™

systEc Zhcr® vacuum pumpIntroducing the ZHCR® (Zero Hysterisis Constant Run) stepper motor driven vacuum pump, specifi cally designed and developed for membrane degassing of HPLC mobile phase and other fl uids used in Analytical Instrumentation.

Employing a micro-stepping closed loop vacuum control strategy permits the pump to maintain a constant vacuum level set-point* by varying the RPM of the stepper motor. Th e pump initially runs at a high speed which provides for a quick pull-down and, as it approaches the vacuum control point, the RPM is gradually reduced until the desired vac-uum level is reached. Th is patented control strategy allows the On-Line Degasser to maintain a virtually constant vac-uum that is unaff ected by varying degassing loads. As a con-sequence, fl uctuations in baseline due to vacuum hysteresis are eliminated by not having the pump repeatedly stop and start as is done in many older and existing systems.

Fluctuations in detector baseline of a single-speed pump compared to the patented technology of the Systec ZHCR® vacuum pump.* UV detector baseline fl uctuations are minimal when compared to traditional stop and start vacuum sources.

*vacuum chamber consists of 480 µl of systec af™ tubing:

flow rate is 1 ml/min, eluent is methanol; wavelength is 215 nm.*50 mmhg for most models and 80 mmhg for prep.

typical degassing fluctuations from vacuum sources

0.072

0.074

0.07

0.076

0.078

0.08

0.082

0.084

competitor vacuum source systec Zhcr®

0 50 100 150 200 250 300 350 400

ab

sorb

ance

(au

)

time (sec)

typical degassing fluctuations from vacuum sources

7

systEc af™ mEmbranETh e new Systec AF™ membrane is 50x more permeable and outperforms the older Tefl on® PTFE membranes used in many other degassing systems today. Th is translates into the ability to use shorter tubing for removal of dissolved gasses.

• ultra-high degassing efficiency

• low volume

• considerably shorter equilibration times

• very easy to prime

• short vacuum pull-down times, typically 30 seconds

• single lumen design for consistent degassing

• inert flow path

• Excellent chemical compatibility flow path

• long lifetime

Plot shows remaining dissolved air in methanol using a selection of Systec Mini-Chambers*. Th e range of chambers and specifi cations off ered provide ample solutions for system designs.

* water and methanol mixtures between 30 % and 70 % methanol

will outgas when more than 38 % dissolved air remains in each of

the solvents. other water and organic mobile phases being mixed

using a low pressure gradient system will undergo similar outgassing.

degassing Efficiency: residual air for systec

mini-chambers (percent dissolved air vs. flow rate).

480 µL MINI Chamber 9000-1004

925 µL MINI Chamber 9000-1009

80%

70%

60%

50%

40%

30%

20%

10%

0%0 1 2 3 4 5 6

per

cen

t rem

ain

ing

dis

solv

ed a

ir in

meo

h

flow rate (ml/min)

9

classic“an all-round degasser for most applications in your lab…”

product fEaturEs• Ultra-high degassing effi ciency• Fits every system• Long life expectancy – 5+ years

continuous (24/7) running capacity• Easy to prime• Extremely quiet• Closed loop vacuum control means

constant vacuum (variable RPM)• ZHCR® patented control eliminates

baseline fl uctuations• Excellent chemical compatibility

fl ow path• Biocompatible fl ow path• Continuous vacuum system monitor-

ing to ensure optimum operational conditions are maintained

• Advanced error and leak checking functions

• 2-pin vacuum level validation output• CE & RoHS compliant

thE first choicEWith the highly permeable Systec AF™ membrane an internal degasser chamber volume of only 480 µl is suffi cient to give you excellent degassing effi ciency up to fl ow rates of approximately 3 ml/min. Th e time the liquid spends inside the vacuum chamber is correlated to the degassing effi ciency, so with higher fl ow rate the degassing effi ciency will be low-ered. In many cases the 480 µl vacuum chamber can be used at 5 ml/min with suffi cient degassing.

dEgasi classic is the first choice for most applications in analytical instrumentation and chromatography.this state-of-the-art stand alone degasser will provide you with trouble free and efficient degassing day after day.

100 to 240 vac (±10 %), 1 a,

50 to 60 hz (±3 hz)

wall sockets: 4 supplied with ac adapter, interchangeable: north america/Japan, u.K., continental Europe, australia

POWER REQUIREMENT

USING SUPPLIED AC ADAPTER

Degassing Channel Tubing:

systec af™ (0.045’’ id)

Degassing Channel Pressure Rating:

70 psi (testing pressure)

Wetted Materials:

systec af™, pEEK, glass-filled ptfE

Liquid Connection:

1/4’’-28 unf threaded flat-bottom port

Size (L x H x B):

263 x 131 x 73 mm

GENERAL SPECIFICATIONS

Part NumberNumber of Channels

Internal Volume

0001-6352-a 2 480 µl

0001-6353-a 3 480 µl

0001-6354-a 4 480 µl

0001-6355-a 5 480 µl

11

gpc“when you are using 100 % organic solvents in your fl uidic line…”





fl ow path• Continuous vacuum system monitor-




what is uniQuEDEGASi GPC uses the same 480 µl Systec AF™ degassing membrane as used in DEGASi Classic. Th e main diff erence compared with the DEGASi Classic is that we use a stented version of the vacu-um chamber in the DEGASi GPC.

Th e stent is a short piece of a stainless steel tube placed inside the ends of the degassing membrane, in order to make the internal ferrule in the bulkhead to get a better grip. Th is solution makes an even more secure internal connection when working with 100 % organic solvents in the fl uidic line.

dEgasi gpc is the right choice of degasser if you are working with 100 % organic solvents in your fluidic line. Example of application areas where this degasser is successfully used are gpc (gel permeation chromatography) and normal phase chromatography.

100 to 240 vac (±10 %), 1 a,

50 to 60 hz (±3 hz)


POWER REQUIREMENT






Wetted Materials:

systec af™, pps, glass-filled ptfE,

stainless steel

Liquid Connection:


Size (L x H x B):

263 x 131 x 73 mm



Internal Volume

0001-6622 2 480 µl

0001-6623 3 480 µl

0001-6624 4 480 µl

0001-6625 5 480 µl

13

micro





fl ow path

are you working with very small flow rates? do you want to minimize the dead volumes inside your system?

then dEgasi micro is your number one choice of degasser. with only 100 µl internal volume you minimize the internal volume while still keeping excellent degassing efficiency up to approximately 0.5 ml/min.

100 to 240 vac (±10 %), 1 a,

50 to 60 hz (±3 hz)


POWER REQUIREMENT






Wetted Materials:


Liquid Connection:


Size (L x H x B):

263 x 131 x 73 mm

GENERAL SPECIFICATIONS• Biocompatible fl ow path• Continuous vacuum system monitor-




“an outstanding degasser for very small fl ow rates...”


Internal Volume

0001-6352-s 2 100 µl

0001-6353-s 3 100 µl

0001-6354-s 4 100 µl

0001-6355-s 5 100 µl

15

sEmi-prEp

when working with higher flow rates, up to 6 ml/min, we strongly recommend dEgasi sEmi-prEp equipped with 925 µl degassing chambers. these chambers can be used up to 10 ml/min but with a bit lower degassing efficiency.

“manages higher fl ow rates up to 6–10 ml/min…”

100 to 240 vac (±10 %), 1 a,

50 to 60 hz (±3 hz)


POWER REQUIREMENT






Wetted Materials:


Liquid Connection:


Size (L x H x B):

263 x 131 x 73 mm



Internal Volume

0001-6352-l 2 925 µl

0001-6353-l 3 925 µl

0001-6354-l 4 925 µl

0001-6355-l 5 925 µl





fl ow path• Biocompatible fl ow path• Continuous vacuum system monitor-




17

prEp

the dEgasi prEp vacuum degassing system for analytical instrumentation and hplc removes dissolved gasses at flow rates up to approximately 50 ml/min per channel. built for the rigours of modern preparative and semi-prep scale hplc, its unique design assures reliable continuous operation and the highest level of continuous performance available. the low internal volume of each systec af™ channel offers quick equilibration and short startup times compared with ptfE degassers.

product fEaturEs• Ultra-high degassing effi ciency• Low volume, easy to prime • Dual lumen design for low-fl ow

resistance• ZHCR® patented control eliminates

baseline fl uctuations• Inert fl ow path• Long life expectancy – 5+ years

continuous (24/7) running capacity• Continuous vacuum system mon-

itoring to ensure optimum opera-tional conditions are maintained

tips! we do supply degassing solutions for even higher flow rates. please contact us for further information.

“the best degassing effi ciency for demanding applications”


Channel Volume

(ml)

Flow Rate Per Channel for 70% Gas Removed from 60% MeOH/40% water

(ml/ min)

Pressure Drop (mmHg/ml/min)

0001-6482 2 8.4 25 0.28

0001-6484 2 13.8 50 0.47

100 to 240 vac (±10 %), 1 a,

50 to 60 hz (±3 hz)


POWER REQUIREMENT






Wetted Materials:

systec af™, pps, glass-filled ptfE

Liquid Connection:


Size (L x H x B):

296 x 163 x 79 mm


19

compact

biotech dEgasi compact is a new line of stand alone degas-sers which combine the cutting edge systec technology with a very small footprint at an affordable cost.

DEGASi Compact is available with 2, 4 or 6 degassing channels in a housing with only 167 x 56 mm footprint.

Th is is possible due to the devel-opment of a new single stage vacuum pump with a small built in control board and new 285 µl Systec AF™ vacuum chambers.

Closed-loop control with a contin-uously running vacuum pump gives a very smooth baseline. Th e speed of the pump is varied to maintain an exact vacuum level inside the vacuum chambers.

Th e degasser is equipped with built in error detection functionalities. A bi-color diode on the front panel gives information about the performance. Th e extremely low internal volume of 285 µl provides very fast priming and start up. Despite the small size of the unit it has an expected life time of more than 6 years.

“stand alone on-line degasser with the smallest footprint available”




70 psig

Wetted Materials:

systec af™, pps, pEEK and glass-filled

ptfE

Vacuum Housing Material:

glass-filled pps (polyphenylene sulfide)

Liquid Connections:

1/4’’- 28 unf-1b

Degasser Nominal Performance:

2 ml/min per channel

Expected Lifetime:

>6 years (continuous run @ 100 rpm

12 hours/day 365 days/year)


Part NumberNumber of

channelsInternal volume

0004-2285 2 285 µl

0004-4285 4 285 µl

0004-6285 6 285 µl

20

wE do all thE worK you taKE all thE crEdit

W I T HYOURLOGO

If you have troubles with bubbles in your system – contact

us and we will help you fi nd a solution. High fl ow, low fl ow,

1 channel or 8 channels, nasty solvents – we will customize a

degasser for you. We supply OEM degassers to international

instrument manufacturers around the globe.

• Development

• Design & drawings

• Brackets

• Cables

• Assembling

• Testing

• Warehousing

• IDEX Authorized Service Center

WE HELP YOU EVERY STEP OF THE WAY:

…just add your logo – customized OEM-degassers

20

21

YOUR LOGO NO.1DEGASSER

22

• Wide selection of fi ttings and tubing

• Filters, tools, tubing markers and accessories

• Labeling with your logo and company details

• Packaging in boxes or plastic bags

• Long experience in this business

• We help you to fi nd solutions for your needs

wE hElp you EvEry stEp of thE way:

dEgassEr tubing Kits

alternative fittings for the 1/8’’ od tubing

alternative fittings for the 1/16’’ od tubing

super flangeless

flangelessflangeless

flangeless

super flangeless one-piece superflangeless

one-piece superflangeless

Part Number Degasser Tubing Kits For 1/8’’ OD Tubing (1/4’’-28 Threaded Nuts)

0704 tubing, 5 m fEp tubing 1/8’’ od x 1/16’’ id

1208 flangeless fittings, 10 pcs pps nuts + 12 pcs EtfE ferrules 1/8’’ id

1408 superflangeless fittings, 10 pcs pEEK nuts + 10 pcs EtfE ferrules 1/8’’ id

1608 one-piece superflangeless fittings, 10 pcs pEEK nuts with integrated pEEK ferrule 1/8’’ id

Degasser Tubing Kits For 1/16’’ OD Tubing (1/4’’-28 Threaded Nuts)

0716 tubing, 5 m fEp tubing 1/16’’ od x 0.75mm id

1216 flangeless fittings, 10 pcs pEEK nuts + 12 pcs EtfE ferrules 1/16’’ id

1416 superflangeless fittings, 10 pcs pEEK nuts + 10 pcs EtfE ferrules 1/16’’ id

1616 one-piece superflangeless fittings, 10 pcs pEEK nuts with integrated pEEK ferrule 1/16’’ id

ready-to-send kits with the tubing in desired lengths and the fittings you need; attached, labeled and packed! we help you choose the best fittings and tubing for every application!

NEW!

NEW!

23

onE ExamplE of our tubing Kits

mobilE phasEbottlE Kits

With a production of 15,000 Mobile Phase Bottle Kits per annum we believe we can off er you a high quality concept used by leading LC producers and consisting of 4 sets of FEP tubings with 10 µm SS mobile phase fi lter (other porosities upon request) and Upchurch original Super Flangeless Fittings + mobile phase bottle caps + colored tubing markers. Every tubing is color coded and so is the nut. Kits are mounted and ready to be used – individ-ually packed into PE bags. Standard length is 1.4 meter – other lengths upon request.

bottle cap blue gl45, for 1/8’’ od tubing 2 hole + 1 luer hole 4 set

bottle cap plug, luer 4 pcs

super flangeless ferrules EtfE for 1/8’’ od tubing, yellow 4 pcs

nut super flangeless 1/8’’ pEEK blue 1 pcs

nut super flangeless 1/8’’ pEEK yellow 1 pcs

nut super flangeless 1/8’’ pEEK green 1 pcs

nut super flangeless 1/8’’ pEEK orange 1 pcs

fEp tubing, 1/8’’ od, 1/16’’ id, per m 4 x 1.4 m

solvent filter ss 10 µm with stem for 1/16’’ id tube (40 ml/min) 4 pcs

marker tube blue 1 pcs

marker tube yellow 1 pcs

marker tube green 1 pcs

marker tube orange 1 pcs

contEnt of thE Kit (p/n Kit.b.10.4): We tailor tubing kits to suit your requirements…

24

Biotech AB

P.O. Box 133

SE-439 23 Onsala

Sweden

Telephone +46 (0)300-56 91 80

Fax +46 (0)300-56 91 81

[email protected]

www.biotech.se

Distributor:

Biotech Sweden is a company highly specialized in chroma-tography accessories and consumables. Biotech is located on the Swedish Westcoast – south of Gothenburg – at the beautiful Onsala peninsula. We at Biotech believe that value is not ”the lowest price” – our customers are the reason we exist – our customers always come first. We have not lost sight where we come from and there-fore we try harder to be your partner, we’re not satisfied unless you are satisfied. Thank you for your business. I know we’ll continue to grow and prosper together.

a simplE philosophy...

USA Biotech USA – [email protected] INDIA Biotech Pvt Ltd – [email protected] SWEDEN Biotech AB – [email protected]

”IDEX Health & Science LLC is pleased to announce the appointment of Biotech AB the designation of Tier 1 European Distributor effective from January 1, 2011. As a Tier 1 Distributor, Biotech AB will provide an increased level of ser-vice to our European customers through technical support, superior customer focus, and localized delivery capabilities.” – IDEX Health & Science LLC

ww

w.s

om

mar

.se

EliminatEs troublEs with bubblEs - Biotech · 2020. 9. 17. · debubblers - contact us! no troublEs...

Documents

Transcript of EliminatEs troublEs with bubblEs - Biotech · 2020. 9. 17. · debubblers - contact us! no troublEs...