Echo 525 Liquid Handler - Echo Qualified Reservoir Quick ... · Reservoir plate is used to add a...
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Echo ® 525 Liquid Handler - Echo ® Qualified Reservoir Quick Start Guide Version 1.1 The Echo ® Qualified Reservoir conveniently and affordably transfers large reagent volumes on the Echo ® 525 Liquid Handling System. The low dead volume of the Echo Qualified Reservoir enables scientists performing genomic, proteomic, and cell based assays to efficiently dispense costly and precious reagents and samples. The maximum working volume for each chamber is 2.55 mL and the dead volume is less than 10%. Constructed of polypropylene, the Echo Qualified Reservoir is compatible with a broad variety of aqueous solutions. The Echo Qualified Reservoirs have been manufactured in an DNase and RNase-free environment. The Echo Qualified Reservoir meets the Standards ANSI/SLAS 1-2004 through ANSI/SLAS 3-2004 (small flange) and can be filled by manual or automated methods. The welded lid minimizes evaporation and spills, and reduces the potential for cross-contamination. The 2.10 mm source plate insert (part #001-11366) should be used with the Echo 525 liquid handler plate gripper. For more information, see the Echo 525 Liquid Handler User Guide. Specifications for the Echo Qualified Reservoir The specifications for the Echo Qualified Reservoir Plate are listed below: 1 Varies with fluid properties. May be lower. 2 Recommended to remove bubbles caused by the filling process or reagent outgassing. Echo Qualified Reservoir Dead Volume (μL) 1 250 μL Max Fill Volume (μL) 2,800 μL Wicking No limitation Centrifugation Recommendation 2 1500 g's for 5 min Echo Insert Type 2.10 mm BP2 Surfactant 0-2% CMC GPSA2 Surfactant 2-30% CMC GPSB2 Surfactant 30-50% CMC
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Echo® 525 Liquid Handler - Echo® Qualified Reservoir Quick Start GuideVersion 1.1
The Echo® Qualified Reservoir conveniently and affordably transfers large reagent volumes on the Echo® 525 Liquid Handling System. The low dead volume of the Echo Qualified Reservoir enables scientists performing genomic, proteomic, and cell based assays to efficiently dispense costly and precious reagents and samples. The maximum working volume for each chamber is 2.55 mL and the dead volume is less than 10%.
Constructed of polypropylene, the Echo Qualified Reservoir is compatible with a broad variety of aqueous solutions. The Echo Qualified Reservoirs have been manufactured in an DNase and RNase-free environment. The Echo Qualified Reservoir meets the Standards ANSI/SLAS 1-2004 through ANSI/SLAS 3-2004 (small flange) and can be filled by manual or automated methods. The welded lid minimizes evaporation and spills, and reduces the potential for cross-contamination.
The 2.10 mm source plate insert (part #001-11366) should be used with the Echo 525 liquid handler plate gripper. For more information, see the Echo 525 Liquid Handler User Guide.
Specifications for the Echo Qualified ReservoirThe specifications for the Echo Qualified Reservoir Plate are listed below:
1 Varies with fluid properties. May be lower.2 Recommended to remove bubbles caused by the filling process or reagent outgassing.
Echo Qualified Reservoir
Dead Volume (μL)1 250 μL
Max Fill Volume (μL) 2,800 μL
Wicking No limitation
Centrifugation Recommendation2 1500 g's for 5 min
Echo Insert Type 2.10 mm
BP2 Surfactant 0-2% CMC
GPSA2 Surfactant 2-30% CMC
GPSB2 Surfactant 30-50% CMC
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Quick Start Guide
Calibration and Buffer Types Compatible with the Echo Qualified ReservoirThe following calibration and buffer types are compatible with the Echo Qualified Reservoir.
* Volume ranges and working volumes may vary with fluid type.1 Critical Micelle Concentration
Filling the Echo Qualified ReservoirEach well can be filled manually or with automation. The maximum fill volume of each well is 2.8 mL. Care should be exercised to avoid over-filling or using high flow rates that would cause splashing. Overfilling the well may lead to inaccurate droplet ejection, poor droplet placement, or even ejection failure.
Emptying the Echo Qualified ReservoirThe Echo Qualified Reservoir is designed to be single use. After dispensing, the remaining fluid can be recovered using a micropipette or pipette. The dead volume is 250 μL or less per well.
Note: Not all dead volume can be recovered. Centrifugation at 300 g for 1 minute can aid in recovering additional fluid.
Centrifuging the Echo Qualified ReservoirAfter filling the Echo Qualified Reservoir, it should be centrifuged (1,500 x g) for 5 minutes to normalize the liquid meniscus and to remove bubbles in the liquid. The Echo Qualified Reservoir lid allows for centrifugation without the fluid spilling from the plate and flowing into adjoining wells. Bubbles in the fluid prevent the formation of an even fluid meniscus. An uneven fluid meniscus can cause imprecise fluid transfer.
Echo 525 Liquid Handler SoftwareThe Echo 525 liquid handler client software that comes standard with the Echo instrument is designed for 384-well and 1536-well plate replication protocols. However, the Echo 525 liquid handler software does not currently support the 6-well Echo Qualified Reservoir plate. To use the 6-well Echo Qualified Reservoir plate that is configured on some Echo 525 liquid handlers, one of the following applications must be installed on the client: Echo Plate Reformat application, Echo Cherry Pick application, Echo Dose Response application, or Echo Combination Screen application.
Echo Plate Reformat ApplicationThe Echo Plate Reformat application gives you flexibility in designing assay plate formats that can take full advantage of the 6-well Echo Qualified Reservoir plate and a number of source plate configurations. The example below shows you one of the many protocols you can create using Echo qualified source plates and the 6-well Echo Qualified Reservoir plate.
Plate Calibration Volume Range (μL)* Working Range (μL)*
Echo Qualified Reservoir 6RES_AQ_BP2 (buffer 0 - 30% V/V glycerol and
0-2.5% CMC1 surfactant)
250 - 2,800 2,550
Echo Qualified Reservoir 6RES_AQ_GPSA2 (buffer 0 - 30% V/V glycerol and
2.5-30% CMC1 surfactant)
250 - 2,800 2,550
Echo Qualified Reservoir 6RES_AQ_GPSB2 (buffer with 0 - 30% V/V
glycerol and 30-50% CMC1 surfactant)
250 - 2,800 2,550
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Quick Start Guide
Creating a ProtocolIn this example, a column of samples is replicated to every column of a 384-well assay plate, and the 6-well Echo Qualified Reservoir plate is used to add a reagent to every well of the assay plate. For a complete list of capabilities, please refer to the Echo Plate Reformat User Guide.
The high-level steps used to create a typical transfer protocol using Echo Plate Reformat application are shown below:
1. Start a new protocol.
2. Select the source and destination plate types.
3. Select a custom mapping mode.
4. Select a source region to copy or replicate on a destination plate.
5. Select a destination region where the source region will be copied or replicated to.
6. Add or replicate a region.
7. Add a fixed plate (6-well Echo Qualified Reservoir plate) to the protocol.
8. Select a source well to replicate on the destination plate.
9. Select a destination region where the source well will be replicated to.
10. Replicate the region.
11. Save and run the protocol.
A specific example of how to create a 384-well replication protocol using a 6-well reagent reservoir plate for the Echo 525 liquid handler using Echo Plate Reformat application is shown below.
To transfer the contents from a single column to multiple row/columns in a destination plate:
1. To start a new protocol, select New from the File menu.
2. To select the source and destination plate types, in the Protocol Setup window, select the Plate Format to be 384PP or 6RES. Based on the plate format chosen, the compatible plate types appear for Default Plate Type.
3. Select the Default Plate Type to set the transfer properties for the Source Plate.
4. Select the Plate Type to set the transfer properties for the Destination Plate.
Note: Not all plate formats and plate types are available on all instruments. The specific plate requirements per user
environment are specified in the purchase agreement and programmed into the instrument prior to shipping.
5. In the Protocol Setup window, ensure Custom is selected for the mapping mode.
6. Click OK to close the Protocol Setup window. The Echo Plate Reformat application maps the plates and displays them in the main Protocol window.
Note: All custom settings are on the main Protocol window in the Custom mapping protocol tab.
7. In the main Protocol window, select wells within a column as the region in the source plate map. The information box for that region automatically opens. For this example, select column 8.
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Quick Start Guide
8. Select multiple wells for the destination region in the destination plate map. For this example, select the entire destination plate.
Note: The number of wells for the region in the destination plate must be greater or equal to the number of wells for
the region in the source plate. If there are less, an error message is displayed when the Replicate Region button is
clicked.
9. Enter the Volume (nL) to be replicated to the destination wells. The default value is 25 nL. For this example, enter 1000 nL for the volume.
10. Click the Replicate Region button or select the Replicate Region icon in the Toolbar.
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Quick Start Guide
11. Select Fixed in the Protocol Plates tree in the main Protocol window.
12. Right click on the Fixed node and select Add Plate.
13. In the Fixed Plate Setup box, select the Format and the Default Plate Type and click OK.
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14. Select the new Fixed Plate in the Protocol Plates tree in the main Protocol window.
Note: You can change the Fill Volume in the Plate Information section. In this example, the volume was changed from
1000 μL to 2000 μL.
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15. Select the well in the source plate to be replicated to the destination plate.
16. Select the region in the destination plate that will receive the controls.
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Quick Start Guide
17. Select the Replicate Region icon in the Toolbar.
The controls in the fixed plate will be added to the entire destination plate, including replicates.
18. Optionally, enter the protocol Name and Description.
19. Select Save from the File menu or click the Save icon in the Toolbar.
20. Select Run from the Protocol menu or click the Run icon in the Toolbar.
21. Select the Instrument if it is not already selected.
22. In the Source Plate section select Full plates or Partial plates:
• If selecting the Full Plates option defined by the protocol, specify the number of source plates to process.
• If selecting the Partial Plates option, the user can pick specific plates. When the Partial Plates option is selected, an Import button is displayed to import a .csv pick list indicating the wells to be transferred.
23. The Destination Plate section is only available in Echo Plate Reformat software versions 1.5.3 or later. Changing the value for Plate copies will override the number of plate copies for the protocol.
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Quick Start Guide
24. In addition, the Plate Calculator button is only available in Echo Plate Reformat software versions 1.5.3 or later. The user can click on the Plate Calculator button to view the number of plates needed to run the protocol.
25. Optionally, click Simulate to test the run.
Note: Running a simulation is recommended to verify the transfers defined in the protocol before a live run.
26. Click Run to execute the protocol.
Published October 2015 Part Number: 001-11060 Revision 1.1 Documentation for Echo® Qualified Reservoir, Version 1.0 The information contained in this guide is subject to change without notice. Any unauthorized changes or modifications to the Echo® Qualified Reservoir will void its warranty. Contact Labcyte, Inc. prior to making any change or modification. Copyright © 2015 Labcyte Inc. All rights reserved. Printed in the United States of America. Microsoft, Windows, Windows 7, ActiveX, and Microsoft SQL Server are either registered trademarks or trademarks of Microsoft Corporation in the United States and/ or other countries. Pentium III and Pentium 4 are trademarks or registered trademarks of Intel Corporation or its subsidiaries in the United States and other countries. All other registered or unregistered trademarks used herein are the exclusive property of their respective holders. For research purposes only; not for use in diagnostics.
The Echo liquid handler is covered by one or more of the following patents: United States: 6,416,164; 6,548,308; 6,603,118; 6,612,686; 6,642,061; 6,666,541; 6,707,038; 6,710,335; 6,746,104; 6,802,593; 6,808,934; 6,809,315; 6,849,423; 6,855,925; 6,869,551; 6,893,836; 6,893,115;6,916,083; 6,932,097; 6,938,987; 6,938,995; 6,991,917; 7,070,260; 7,090,333; 7,185,969; 7,270,986; 7,354,141; 7,405,072; 7,405,395; 7,439,048; 7,454,958; 7,481,511; 7,717,544; 7,899,645; 7,900,505; 7,901,039; 8,107,319. European Patent EP: 1322430; 1324823; 1337325; 1352112; 1366356; 1534526; 1585636. Japan: 4189964; 4309131; 4434581; 4559218; 4624644; 4955901; 4990476. Additional patents are approved and pending in the United States and other countries. Labcyte Inc.
