DNA Replication

DNA Replication:

SEMICONSERVATIVE MODEL

FIG 16.10

This process is fueled by…nucleoside nucleoside triphosphatestriphosphates

Semi-conservative

DNA is made from DNA is made from 5’ to 3’5’ to 3’ and it is and it is read from 3’-5’.read from 3’-5’.

The 3’ end is the end which elongates (grows)

Leading & Lagging strands

•made 5’-3’

Okazaki fragments (are of the lagging strand)

ENZYMES:

•Helicase (not shown)

•DNA Polymerase

•ligase

Enzymes

•Helicase

•Single strand binding proteins

•Primase(RNA Primer)

•DNA Polymerase

•Ligase

•Nuclease and DNA Polymerase (both are repair enzymes)

Repairing mistakes

Telomeres

•Unfilled gap left at the ends of the DNA strands due to the use of RNA primers

•Eventual shortening of DNA over time

•Dolly, cancer, telomerase