DNA BIOLOGY PPT_Application.pdf · GPQ 05 G-Quant Bradford plus compatible protein assay Reagent...

DNA BIOLOGY

RNA BIOLOGY

PROTEIN BIOLOGY

MOLECULAR BIOLOGY

OLIGO SYNTHESIS SERVICE

CUSTOM SERVICES

DNA BIOLOGY

Genomic DNA Isolation Kits

Custom DNA Isolation Kit Soil, Stool, Hair, Urine Sputum, Tear, Milk, Water, Forensic, NEWHoney, NEWApototic Ladder Detection

Tissue DNA Isolation

Blood & Cell Culture

Direct Blood & Serum

Plants & Seeds

Micro-Organisms

Total DNA Isolation

G46221 GSure Fast Tissue Kit G46221F GSure FFPE Tissue DNA Kit

G4624A GSure Blood & Cell Culture DNA Mini Kit

G4625 GSure Blood DNA Mini Kit G45271 GSure Serum DNA Isolation Kit

G4620 GSure Plant Mini Kit G4620WLN GSure Plant Mini Kit with WLN buffer G4532 GSure Plant Mini Kit For Seed G4619M GSure Plant Maxi Kit

G46211 GSure Bacterial Genomic DNA isolation Kit G45331 GSure Fungal DNA kit G45333 GSure Algae DNA kit

G4621 GSure DNA mini kit

DNA Biology

DNA Isolation Kits

Genomic DNA Isolation

Plasmid DNA Isolation

Mitochondrial DNA Isolation

DNA Isolation Reagents

DNA Purification DNA Gel

electrophoresis Reagents

DNA Stabilization Solution


G46121 GSure plasmid Mini kit G46122 GSure Splash Plasmid mini kit G4615 GSure Plasmid Midi Kit G4617 GSure Plasmid Maxi Kit G46123 NEWGSure Rapidplasmid Mini kit


GM101 NEWMitochondrial DNA isolation kit

DNA Biology

DNA Isolation Kits








GCR-2 Phenol:Chloroform:Isoamyl alcohol-Molecular biology grade

GCR-3 Chloroform:Isoamyl alcohol-Molecular biology grade

GCR-4 Tris saturated Phenol (pH 8)-Molecular biology grade

GCR-7 Alkaline lysis solution pack (Solution 1, Solution 2 & Solution 3) (for Plasmid isolation)

GCR 9 Lysozyme (10 mg/ml) in 10 mM Tris-HCl, pH 8.0 (Molecular biology grade)

GEB9A 2X Lysis/ equlibration buffer

GWB9A His Protein Purification-Wash Buffer

G474B Proteinase K solution (20mg/ml)-Molecular biology grade

G4633 Ribonuclease A solution (RNAse A), (20mg/ml)-Molecular biology grade

GCR-12 CTAB lysis solution (Molecular biology grade)

GRBC01 RBCL Buffer


DNA/ PCR Purification

G4628A GSure PCR Purification Kit G4629A GSure Gel Extraction Kit G78200 ExoSap-Seq Kit (for enzymatic clean-up)

DNA Biology

DNA Isolation Kits








DNA Electrophoresis Reagents

GCR-17 6x Gel loading dye (Single dye) GCR-19 6x Gel loading dye (double dye) GCR-21 6X DNA Loading Dye (Triple Dye) GCR-23 6X Orange DNA Loading Dye G4663 20 bp DNA Step Ladder G4666 50 bp DNA Step Ladder G4667 100bp DNAStep Ladder G4668 200bp DNAStep Ladder G4669 1kbDNAStep Ladder GCR-28 Ethidium Bromide solution (10mg/ml) G4708 Super Stain Nucleic Acid Gel Stain (20000X) G4687 10X TBE Buffer -HI-grade G4691 1X TBE buffer,-HI-grade G4692 10X TAE Buffer -HI-grade G4696 1X TAE buffer-Hi grade G4651 High Gel Agarose NEW, MB grade (DNA & RNA free) G4650 LMP Agarose


G8002 DNA Later

G8002A DNA Later

G4704 DNA Safe

Applications of DNA BIOLOGY

DNA Quality Check in gel

Related Products GCC portfolio Agarose LMP, STR, High Gel Gel Running Buffer TAE, TBE DNA Ladder 20bp, 50 bp, 100 bp, 1kb

DNA Gel Stain Super Stain Nucleic Acid Gel Stain ; Ethidium Bromide Gel Loading Dye 6X, Hi-6X for single, double and triple dye Nuclease Free Water MB grade water

DNA Stabilization Solution DNA Later, DNA Safe

DNA Quantification

SYBER Green solution 10000X in DMSO Quant Pico Green dsDNA Quantitation Kit Quant Pico Green dsDNA Quantitation Reagents (Concentrate in DMSO) GQuantEZ Fluorometric ds DNA Quantitation Kit

Applications of DNA BIOLOGY

PCR of DNA

Primer Primer designing

Primer synthesis

Thermo-stable Polymerases

G9 Taq DNA pol and 2X Mix

Hi- G9 Taq DNA pol and 2X Mix

PrimeTaq DNA Pol and 2X Mix

Hi-PrimeTaq DNA Pol and 2X Mix

JetPro Polymerase mix

JetPro Pol Dry Mix

G9 PCR CORE KIT (G9 Taq)

Hot Start G9 Taq DNA Pol and 2X Mix

Hot Start PrimeTaq DNA Polymerase

GPfu DNA Pol and 2X Mix

Pro Pfu DNA Pol (Pfu Turbo) and 2X Mix

2x G9 Taq Readyload PCR master Mix

2X G9 Ecopolymerase readyload Xeropolymerase Dry PCR Mix

DeltaQ Polymerase

1X Colony PCR Master Mix

Other PCR Consumables

PCR grade extra pure Water

PCR grade extra pure MgCl2, 25mM

PCR grade extra pure DMSO

Hi-grade Nuclease free water

Magnesium Chloride (MgCl2), 25 mM

10 mM dNTP mix, ultrapure

RNA BIOLOGY

Bacterial RNA Isolation

Cell Culture

Tissue

Plants & Seeds

Blood

Total DNA Isolation

GR1001 GSure Bacterial RNA isolation Kit

GR1002A GSure Blood RNA isolation Kit

GR1003A GSure Cell Culture RNA isolation Kit

GR1005A GSure Tissue RNA isolation Kit

GR1004A GSure Plant RNA isolation Kit

GR1006A GSure Total RNA isolation Kit

RNA Isolation Kits

RNA Biology

RNA Isolation Kits

Total RNA Small RNA

RNA Isolation Reagents RNA Purification RNA Gel electrophoresis

Reagents RNA Stabilization

Solution

RNA Biology

RNA Isolation Kits

Total RNA Small RNA



Solution

Bacterial Small RNA Isolation

Blood & Cell Culture

Tissue Small RNA

Plants Small RNA

GRS1009A GSure Simplex Bacteria small RNA isolation kit

GRS1008A GSure Simplex Blood & Cultured cell small RNA isolation kit

GRS1006A GSure Simplex Tissue small RNA isolation kit

GRS1007A GSure Simplex Plant small RNA isolation kit

Small RNA Isolation Kits

RNA Biology

RNA Isolation Kits

Total RNA Small RNA



Solution

RNA Isolation Reagents

G31C Trizin RNA extraction reagent G7197 Trizin LS RNA extraction reagent

G47053 MSodium Accetate, (pH-5.2) molecular biology grade

GCR-30 RNA grade Nuclease free water2x

GCR-32 Acid-Phenol:Chloroform, pH 4.5 (with IAA, modify 25:24:1) G4648 RNase Inhibitor (40u per ul) G46481C RNA Later10x G46481A RNA Later G7111 Rnase ZIP

G28 Hi-Pure DEPC-treated water (RNA grade) (0.22u filtered)5x G7196 Ultra Pure DEPC treated water4x

RNA Purification

GR1007 GSure RNA Clean up kit

GR1008 GSure RNA gel purification kit

RNA Biology

RNA Isolation Kits

Total RNA Small RNA



Solution

RNA Gel Electrophoresis Reagents

G7112 RiboStain 2x GCR-C3 10x MOPS Buffer

GCR-5 1M Tris. HCl (pH 8) (Nuclease free)

GCR-6 10mM Tris.Cl buffer (pH 8) (Nuclease free)

GCR-353 RNA Ladder, 20 loading, ssRNA, (0.3kb to 3kb) G4651 High Gel Agarose NEW, MB grade (DNA & RNA free) G4650LM LMP Agarose GCR-A3 2x Ribo load RNA Gel loading dye GCR-C3 10x MOPS Buffer

GCR-D4 Formaldehyde

RNA Stabilization Solution

G46481C RNA Later

RNase Decontamination Solution

G7111 Rnase ZIP

Applications of RNA BIOLOGY

Cat. No. Product Details G7112 RiboStain 2x

GCR-C3 10x MOPS Buffer

GCR-5 1M Tris. HCl (pH 8) (Nuclease free)

GCR-6 10mM Tris.Cl buffer (pH 8) (Nuclease free)

GCR-353 RNA Ladder, 20 loading, ssRNA, (0.3kb to 3kb)

G4651 High Gel Agarose NEW, MB grade (DNA & RNA free)

G4650LM LMP Agarose

GCR-A3 2x Ribo load RNA Gel loading dye

GCR-C3 10x MOPS Buffer

GCR-D4 Formaldehyde


Cat. No. Product Details

G46451A M-MLV Reverse Transcriptase, Rnase H minus

G4645 Max M-MLV Reverse Transcriptase, Rnase H minus

G4641 R2D 1st strand cDNA synthesis kit, Rnase H minus

G4642 R2DFast 1st strand cDNA synthesis kit

G7113 One Step Quick RT PCR Kit

G7114 Dual Step RT PCR Kit

G7114A Dual Step Taq RT PCR Kit

G51 Oligo dT (50uM)

G52 Random Hexamer (50uM)

G52AC Random decomer (50uM)

G52AD Random Octamer (50 uM)


Cat. No. Products Details G7115B G9 Taq DNA polymerase, 10x buffer with MgCl2

GG03 Hi- G9 Taq DNA polymerase G7126 DeltaQ Polymerase

G4798A PrimeTaq DNA Polymerase

G7116B Hi-PrimeTaq DNA Polymerase G7122 GPfu DNA Polymerase


Cat. No. Product Details GCR-54 2x qPCR mastermix, SYBR, Rox

GCR-61 2X H-eff qPCR master mix, Rox GT1001 WRTaqman 2X mastermix from (40ul rxn), 1ml

GFT1001 FasTaqman mastermix for Blood

GCR-65 One step 2x qRTPCR mastermix, SYBR, Rox, 100rxn


Cat. No. Product Details GR47001 T7 RNA polymerase (20U/ul)

GR47011K GmScript T7 Transcription Kit


PROTEIN BIOLOGY

Pro

tein

Bio

logy

Protein extraction Buffer

Protein quantitation kit and reagents

Reagents and Kits for Protein Expression and

Purification

Protein Electrophoresis reagents

Protein Ladder

Protein Staining Dye

LumiFly: ECL Western Blot Kit

Reagents for Isoelectric focusing and 2D gel

electrophoresis

Buffer Cat. No. Product Details

RIPA Buffer

GLB 01 GLyse RIPA Buffer GLB 02 GLyse RIPA Buffer

GLB 03 GLyse RipaPhoslock Buffer GLB 04 GLyse RipaPhoslock Buffer

Bacterial Buffer GLB 05 GLyseB (Bacterial lysis buffer)

GLB 06 GLyseB (Bacterial lysis buffer)

Mammalian Buffer

GLB 07 GLyseM (Mammalian cell lysis buffer) GLB 08 GLyseM (Mammalian cell lysis buffer)

GLB 11 GLyseMT( Mammalian Tissue lysis buffer) GLB 12 GLyseMT( Mammalian Tissue lysis buffer)

Plant Buffer

GLB 09 GLysePL (Plant leaf lysis buffer) GLB 10 GLysePL (Plant leaf lysis buffer) GPA-006 GlysePT (Plant Tissue Lysis Buffer)

GPA-007 GlysePT (Plant Tissue Lysis Buffer)

Animal Buffers GPA-004 GLyseAT (Animal Tissue Lysis Buffer)

GPA-005 GLyseAT (Animal Tissue Lysis Buffer)

Cultured Cell GLB 13 GLyseCC (Protein Extraction Fron Culture Cell) GLB 14 GLyseCC (Protein Extraction Fron Culture Cell)

Cat No. Product Details

GPC-01A:D Hi-bind Ni-NTA agarose bead

GPQ 05 G-Quant Bradford plus compatible protein assay Reagent kit

GPS 101 SARPStain Protein Dye, 100 small gel equivalent

GCR-P3A Unstained Protein ladder unstained (S-14 to 200 Kd)

GIP051 GPure 2D sample preparation kit ( For 20 prep)-Bacteria

GIP052 GPure 2D sample preparation kit ( For 20 prep)-Mammalian

GIP053 GPure 2D sample preparation kit ( For 20 prep)-Plant

G7134 LumiFly ECL Western Blotting Kit

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Pro

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Purification


Protein Ladder




electrophoresis

Cat. No. Product Details GPQ 01 G-Quant Bradford Reagent 1X

GPQ 02 G-Quant Bradford Reagent 1X GPQ 03 G-Quant Bradford Reagent 5X

GPQ 04 G-Quant Bradford Reagent 5X GPQ 05 G-Quant Bradford plus compatible protein assay Reagent kit


GLB 01:02 GLyse RIPA Buffer

GLB 03:04 GLyse RipaPhoslock Buffer

GLB 05:06 GLyseB (Bacterial lysis buffer)

GLB 07:08 GLyseM (Mammalian cell lysis buffer)

GLB 11:12 GLyseMT( Mammalian Tissue lysis buffer)

GLB 09:10 GLysePL (Plant leaf lysis buffer)

GPA 006:007 GlysePT (Plant Tissue Lysis Buffer)

GPA 004:005 GLyseAT (Animal Tissue Lysis Buffer)




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Pro

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Purification


Protein Ladder




electrophoresis


GPC-01A Hi- bind Ni-NTA Agarose Bead GBI-01A G Bond Ni-IDA Agarose Bead

GPIB01 G Pure Inclusion Body purification Kit GPC-016 X-Gal solution, 20mg/ml, Ready to use

GPC-018 100mM IPTG solution, Ready to use GPC-020 5M NaCl

GPC-021 5M Imidazole

GCC-GNS01-25 Gold Nanoparticles, 0.01% Au GCC-GNR01-25 Gold Nanorod, 0.01% Au

GCC-GNF01-25 Gold Nanoflower, 0.01% Au










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Pro

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Purification


Protein Ladder




electrophoresis


GPC-005 2x SDSPAGE sample loading buffer

G4632A 20% SDS Solution

GPC008A 30% Acrylamide:bis-acrylamide solution (29:1)

GPC008C 40% Acrylamide:bis-acrylamide solution (19:1)

GPC-010 Beta-marcaptoethanol

GPC-011 1M Tris.Cl, pH 6.8, for electrophoresis

GPC-012 1.5M Tris.Cl pH8.8, for electrophoresis

GPC-014 Extra pure Water for electrophoresis

GPC-015 Ammonium Per sulfate

GPC-003 10x Tris-Glycine-SDS PAGE running buffer

GPC-004 Ready to use Tris-Glycine-SDS PAGE running buffer

GCR-P1 10X PBS, pH 7.2

GCR-1T 10x TBS buffer for western, Hi-pure

GCR-4P 10x PBST buffer for western, Hi-pure

GCR-1M Fat free dry milk

G7155 DAB (3,3′-Diaminobenzidine tetra hydrochloride)

GPW-003 10X Western Transfer Buffer



GPSP 101 SARPStain Plus Protein gel staining kit (100 small gel)

GPSN 101 G-SNAP in-gel protein visualization reagent (100 small gel)

GSBS 01 SNAP Blue stain

GCR-1W Ponceau S, ready to use protein on membrane staining solution

GCR-P2 Coommassie protein gel staining solution, Ready to use





Related Products

Pro

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Bio

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Purification


Protein Ladder




electrophoresis

Cat. No. Product Details GCR-P3 Unstained Protein ladder (L-14 to 100 Kd)(100 lanes) GCR-P3A Unstained Protein ladder unstained (S-14 to 200 Kd)

GCR-P3B Unstained Protein step ladder (S-10 to 100 Kd)(100 lanes)

GCR-P3SA Unstained protein ladder ( 10 -70 Kd)(100 lanes)

GCR-P3SB Unstained protein ladder ( 5 -50 Kd)(100 lanes) GCR-P3H His-Western Protein Marker (100lanes) GCR-P4 Prestained protein ladder (5-50kDa)(100lanes)

GCR-P4A Prestained protein step ladder (10-100kDa)(100lanes) GCR-P4MC Prestained protein ladder (10-100kDa)(100lanes) GCR-P4B Prime Prestained protein Marker



GBI 01A G-Bond Ni –IDA agarose bead


GPSP 101 SARPStain Plus Protein gel staining kit (100 small gel)

GPSN 101 G-SNAP in-gel protein visualization reagent (100 small gel)






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Pro

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Bio

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Purification


Protein Ladder




electrophoresis

Cat. No. Product Details GPS 101 SARPStain Protein Dye, 100 small gel equivalent

GPSP 101 SARPStain Plus Protein gel staining kit (100 small gel) GPSN 101 G-SNAP in-gel protein visualization reagent (100 small gel)

GSBS 01 SNAP Blue stain GCR-1W Ponceau S, ready to use protein on membrane staining solution GCR-P2 Coommassie protein gel staining solution, Ready to use
















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Pro

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Purification


Protein Ladder




electrophoresis

Cat. No. Product Details G7134 LumiFly ECL Western Blotting Kit G7210 LumiFly Fast ECL Western Blotting Kit

G7211 LumiFly Prime ECL Western Blotting Kit G7134 LumiFly ECL Plus Western Blotting Kit















GCR-P3H His-Western Protein Marker (100lanes)

GCR-P4 Prestained protein ladder (5-50kDa)(100lanes)

GCR-P4A Prestained protein step ladder (10-100kDa)(100lanes)

GCR-P4MC Prestained protein ladder (10-100kDa)(100lanes)

GCR-P4B Prime Prestained protein Marker

Related Products

Pro

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Purification


Protein Ladder




electrophoresis

Cat. No. Product Details GIP02 2D Clean up kit ( For 50 prep) GIP03 2D Rehydration Buffer (Soluble protein)

GIP051 GPure 2D sample preparation kit ( For 20 prep)-Bacteria GIP052 GPure 2D sample preparation kit ( For 20 prep)-Mammalian











GCR-P3 Unstained Protein ladder (L-14 to 100 Kd)(100 lanes)

GCR-P3A Unstained Protein ladder unstained (S-14 to 200 Kd)

GCR-P3B Unstained Protein step ladder (S-10 to 100 Kd)(100 lanes)

GCR-P3SA Unstained protein ladder ( 10 -70 Kd)(100 lanes)

GCR-P3SB Unstained protein ladder ( 5 -50 Kd)(100 lanes)



Related Products

Protein

Extraction

Protein Quantification

Protein Expression and Purification

Isoelectric Focusing and 2D

gel electrophoresis

Protein Electrophoresis

Protein Ladders


LumiFly: ECL Western Blot

Applications of PROTEIN BIOLOGY

MOLECULAR BIOLOGY

Cat. No. Product Details GRD1001A GSure Ultra Nucleic acid isolation Universal kit

GRD1001 GSure Ultra Nucleic acid isolation kit (Bacteria)

GRD1002 GSure Ultra Nucleic acid isolation kit (Cultured Cell)

GRD1003 GSure Ultra Nucleic acid isolation kit (Blood)

GRD1004 GSure Ultra Nucleic acid isolation kit (Plant)

GRD1005 GSure Ultra Nucleic acid isolation kit (Tissue)

Cat. No. Product Details GD1001 GSure Dogma kit for Bacteria

GD1002 GSure Dogma kit for Plant

GD1003 GSure Dogma kit for Cultured Cell

GD1004 GSure Dogma kit for Tissue

GD1005 GSure Dogma kit for Blood

GD1006 GSure One Prep Virus Kit

GD1006 GSure One Prep Virus Kit

DNA & RNA Isolation

DNA, RNA & PROTEIN Isolation

Applications PCR, RT-PCR, real-time PCR, microarray, NGS

Applications PCR, RT-PCR, real-time PCR, microarray, PAGE, 2D, western blotting, NGS


GRDM101 GSure Complete Nucleic acid isolation kit (DNA, RNA & miRNA)

DNA, RNA & miRNA Isolation

Reverse Transcription & cDNA synthesis

M-MuLV Reverse Transcriptase One Step Quick RT-PCR Kit Random primer

G46451A M-MLV Reverse Transcriptase, Rnase H

G4641 R2D 1st strand cDNA synthesis kit, Rnase H minus

G4642 R2DFast 1st strand cDNA synthesis kit

G4642B Prime R2D Double strand cDNA synthesis kit

G7113 One Step Quick RT PCR Kit

G7114 Dual Step RT PCR Kit

G7114A Dual Step Taq RT PCR Kit

G51 Oligo dT (50uM)

G52 Random Hexamer (50uM)

Applications PCR, RT-PCR, real-time PCR, microarray, NGS

Applications RT-PCR, fast cDNA synthesis, real-time PCR, microarray, NGS

Thermo-stable DNA Polymerase

Custom DNA Isolation Kit NEW XeroPolymerase, NEWJetPro Polymerase

APPLICATIONS

DNA Polymerase Selection Chart

+++ indicates recommended choice for application

GCC Biotech product offering for PCR includes a variety of DNA polymerases providing a suitable enzyme for all purposes, from routine PCR to the most demanding high-fidelity applications. The tables below indicate the suitability of different enzymes to specific applications, and some characteristics of the polymerases

Direct PCR Fast PCR Hot Start activity

High-fidelity Applications

TA-Cloning

Low copy number

detectibility

High complexity templates

Long-range PCR

Routine PCR

Site-directed mutagenesis

Master Mix format

Ready-load MM

Dried format

G9 Taq DNA polymerase -- +++ NA -- +++ + + + +++ -- A NA NA

Hi-G9 Taq DNA polymerase -- +++ NA -- +++ + + ++ +++ -- A NA NA

PrimeTaq DNA polymerase -- +++ NA ++ -- ++ ++ +++ +++ ++ A NA NA

Hi-PrimeTaq DNA polymerase -- +++ NA +++ -- ++ +++ +++ +++ ++ A NA NA

JetPro polymerase mix + +++ NA +++ -- +++ +++ +++ +++ ++ NA NA NA

JetPro polymerase Dry Mix + +++ NA +++ -- +++ +++ +++ +++ ++ A NA A

G9 PCR CORE KIT (G9 Taq) -- +++ NA -- +++ + + + +++ -- A NA NA

HS Prime Taq DNA Polymerase -- +++ A ++ -- ++ +++ +++ +++ ++ A NA NA

Hot Start G9 Taq DNA polymerase -- +++ A -- +++ + ++ ++ +++ -- NA NA A

GPfu DNA polymerase -- ++ NA ++ -- ++ -- + -- -- NA NA NA

Pro Pfu DNA polymerase (Pfu Turbo) -- +++ NA ++ -- ++ -- ++ -- -- NA NA NA

2x G9 Taq Readyload PCR master Mix -- +++ NA -- +++ + + + +++ -- A A NA

2X G9 Ecopolymerase readyload -- +++ NA -- +++ + + + +++ -- A A NA

Xero polymerase Dry PCR Mix -- +++ NA -- +++ + + + +++ -- A A A

DeltaQ polymerase -- +++ NA -- +++ ++ ++ + +++ -- NA NA NA

1X Colony PCR Master Mix -- +++ NA -- NA + + + -- -- A A NA

Fastract Direct PCR Kit +++ ++ NA +++ -- +++ ++ -- NA NA A A NA

5'-3' polymerase activity

5'-3' exonuclease activity

3'-5' exonuclease activity

Reverse transcriptase

activity

Shipping Condition

Recommended extension time

G9 Taq DNA polymerase A + -- -- -20°C 1 min/kb

Hi-G9 Taq DNA polymerase A + -- -- -20°C 1 min/kb

PrimeTaq DNA polymerase A + + -- -20°C 1 min/kb

Hi-PrimeTaq DNA polymerase A + + -- -20°C 1 min/kb

JetPro polymerase mix A -- + -- -20°C 1 min/kb

JetPro polymerase Dry Mix A -- + -- -20°C 1 min/kb

G9 PCR CORE KIT (G9 Taq) A + -- -- -20°C 1 min/kb

Hot Start G9 Taq DNA polymerase A + -- -- -20°C 1 min/kb

Hot Start Prime Taq DNA Polymerase A + -- -- -20°C 1 min/kb

GPfu DNA polymerase A -- + -- -20°C 2 min/kb

Pro Pfu DNA polymerase (Pfu Turbo) A -- + -- -20°C 2 min/kb

2x G9 Taq Readyload PCR master Mix A + -- -- -20°C 1 min/kb

2X G9 Ecopolymerase readyload A + -- -- -20°C 1 min/kb

Xero polymerase Dry PCR Mix A + -- -- Room Temp 1 min/kb

DeltaQ polymerase A -- -- -- -20°C 30 sec/kb

1X Colony PCR Master Mix A + -- -- -20°C 45 sec/kb

Fastract Direct PCR Kit A -- + -- -20°C 1 min/kb

CHARACTERISTICS

DNA Polymerase Selection Chart

Fastract Direct PCR Kit

G45311A Fastract Direct PCR Kit for Plant

G45312A Fastract Direct PCR Kit for Blood

G45313A Fastract Direct PCR Kit for Cultured Cell

G45314A Fastract Direct PCR Kit For Tissue

G45315A Fastract Direct PCR Kit for Saliva/Buccal Swab

G45316A Fastract Direct PCR Kit for Forensic samples

G45317A Fastract Direct PCR Kit For Hair

GCR-54 2x qPCR mastermix, SYBR, Rox

GCR-61 2X H-eff qPCR master mix, Rox

GCRS-001 SYBER Green solution 10000X in DMSO

GT1001 WRTaqman 2X master mix

GFT1001 FasTaqman mastermix for Blood

GCR-65 One step 2x qRTPCR mastermix, SYBR, Rox, 100rxn

GCR-4 CUSTOM-TURBO TAQMAN PROBE

GCR-7H Taqman mgb Probe

qRT-PCR Kit

Applications genotyping, paternal testing, genetic testing, veterinary testing, forensic testing

Applications

gene expression analysis, the detection of genetically modified organisms in food, cancer phenotyping, gene copy number (gene dosage), presence of mutant genes, cellular mRNA levels

TA Cloning Kit

G4715A Hi efficiency TA-Cloning kit

G4715AA Hi efficiency TA-Cloning kit with competent cell G4726 T4 Polynucleotide Kinase, Hi-Q

G4719 T4 PNK Buffer

G4720 T4 DNA Ligase

GPL-02A NEWDNA Ligation Kit

G4718 T4 DNA Ligase Buffer

G4724 Klenow Fragment

G4721A Exonuclease I

G4722 Shrimp Alkaline Phosphatase (SAP)

G4725 Alkaline Phosphatase Calf Intestinal (Hi-Q)

GS25A T4 DNA polymerase, Hi-Q

G47 dNTP's (100mM dNTP set)

G4810 mM dNTP mix, ultrapure

GCR-13A 10mM DTT, Hi-pure reaction grade

GCR-13B 100mM MgSO4 reaction grade

G71291M Tris pH 8, Hi-pure reaction grade

G71301M HEPES, pH 6.6, Hi-pure reaction grade

GCR-6A 0.5M EDTA, Hi-pure reaction grade

G4776 X-Gal

G4777 IPTG, Dioxane-

GCR-4IP1 IPTG 20MG/ML

Modifying enzyme and reagents

Competent Cells

GCR4 DHSuper DH 5a Competent Cell

GCR-4XL Super XL-1 Blue Competent Cells

GCR-4NK Efficomp supercompetent E.coli cells

GCR-4BL BL21(DE3) Competent E. coli

GCR-4PL BL21(DE3)pLysS Competent Cells

G7206 Site Directed Mutagenesis Kit S (6Kb)

G7206A Site Directed Mutagenesis Kit S (6Kb) with competent cell

Site Directed Mutagenesis Kit

G4651 High Gel Agarose NEW, Molecular biology grade (DNA & RNA free)

G4650 LMLMP Agarose

G4657 ISSR Agarose

G4658 STR Agarose

Agarose

GCR-5 1M Tris.HCl (pH 8) (Nuclease free) GCR-6 10mM Tris.Cl buffer (pH 8)(Nuclease free) G4674 TE buffer (pH 8)-Molecular biology grade

G4635 Hi-grade Nuclease free water

G7192 10% SDS solution (UP) G4632A 20% SDS solution (Hi-grade) G47053 MSodium Accetate, (pH-5.2)-Molecular biology grade

GCR-1

Isopropanol Extrapure, for DNA/RNA isolation-Molecular biology grade

G4675 TE Buffer (Molecular biology grade) G4677 0.5M EDTA,pH8.0 (Molecular biology grade) G4679 0.1 M EDTA (pH-8) (Molecular biology grade) G4680 1MTris,pH7.0 (Molecular biology grade) G4682 1MTris,pH8.0 (Molecular biology grade) G4684 1MTris,pH6.8 (Molecular biology grade) G4685 1.5MTris,pH8.8 (Molecular biology grade)

G4686

1MTris buffer Pack,pH6.8,pH7.0,pH7.5, pH8 (Molecular biology grade)

G4697 SSC Buffer

G4698 Hi-grade DEPC-treatedwater (Molecular biology grade) G4764 BCIP/NBT Color Development Substrate G4764A TMB/H2O2 Chromogenic substrate solution

GCR-1N 20X SSC Buffer500ml7800GCR-2 N20X SSC, Hi-pure

GCR-3N 10N NaOH

GCR-6N 2x Hybridization buffer GCR-7N 2x Hi-Prime hybridization buffer

GCR-8N

Hi-Pure Southern blotting buffer set,(Depurination, Denaturation & Nutralization), 500ml each3x

G4736 Glycine

G4738 Sodium Dodecyl Sulfate

G4744 Tris Base

G4747 Triton X-100500ml11772G4748T riton X-100

G4749 Tween 20500ml9632G4750Tween 20

G4751 Ammonium Sulfate

G4752 Sodium Chloride

G4754 HEPES (free acid) G4756 Guanidine-HCl G4758 Glycerol G4761 Urea

G4763 Bovine Serum Albumin, Acetylated G4767 Acrylamide Molecular Grade

G4769 Bisacrylamide, (N,N'-Methylenebisacrylamide) G4771 DTT, (Dithiothreitol) G4774 Urea

G477 6X-Gal G4777 IPTG, Dioxane-Free

G7138

EGTA(Ethylene Glycol bis-(2-aminoethyleether)-N,N,N',N'-tetra acetic acid)

G7139 Phenylmethanesulphonyl fluoride

G7140 Protease inhibitor cocktail (100x) G4881 DpnI

GCR-A13 PCR grade extra pure Water

GCR-B13 PCR grade extra pure MgCl2 25mM

GCR-4PG PCR grade extra pure DMSO

G4635 Hi-grade Nuclease free water

G4635A Hi-grade Nuclease free water

GCR-A3 MMagnesium Chloride (MgCl2) Molecular Biology Grade, 25 mM

GCR-13A 10mM DTT, Hi-pure reaction grade

GCR-13B 100mM MgSO4, reaction grade

MB grade buffers & reagents

GC1001 GProFect Transfection reagent

GC1002 GProFect Transfection reagent 5x1ml

New Product

Sequencing grade reagents

G78200 ExoSap-Seq Kit (for enzymatic clean-up, best use for sequencing reaction) 500rxn

G78201A Sequencing grade Ethanol Precipitating Solution

G78202 Sequencing grade water

G78202B Sequencing grade DEPC Treated water

G4705B 3M Sodium Accetate, (pH-5.2), molecular biology grade, DNAse & RNAse free

G4677B 0.5MEDTA, pH8.0

G4679P Sequencing Grade PCR Master Mix

G4679MA Sequencing Grade Mgcl2 Solution 25mM

G4679DA Sequencing Grade 10 mM dNTP mix

Cat. No. Details Specifications

GC-01 Oligo/Primer (11 – 39 bases) 25nmol GC-01A Oligo/Primer (11 – 39 bases) 10nmol

GC-02 Oligo/Primer (11 – 80 bases) 50nmol GC-03 Oligo/Primer (5 – 100 bases) 200 nmol GC-04 Oligo/Primer (5 – 100 bases) 1000 nmol

GC-05 Oligo/Primer (5 – 100 bases) 10000 nmol

GC-06 HPLC Purification Charges per oligo 50 nmol

HPLC Purification Charges per oligo 200 nmol HPLC Purification Charges per oligo 1000 nmol HPLC Purification Charges per oligo 10000 nmol

GC-07 PAGE Purification 50 nmol PAGE Purification 200 nmol

PAGE Purification 1000 nmol PAGE Purification 10000 nmol GC-08 SWIFT LC Charges 25 nmol

SWIFT LC Charges 50 nmol SWIFT LC Charges 200 nmol

SWIFT LC Charges 1000 nmol SWIFT LC Charges 10000 nmol

Oligo Synthesis

OLIGO SYNTHESIS

Custom Services

Will discuss in details in upcoming presentations..

GSureTM Splash plasmid DNA Isolation Kit

2x H-eff qPCR Master Mix ROX 2x qPCR Master Mix SYBR ROX GSureTM Dogma kit for plant

ECL PLUS GSureTM Gel Extraction Kit GSureTM PCR purification Kit GSureTM Plant Mini kit with WLN Buffer

IN Gel SNAP Blue SARP stain GSureTM Simplex kits for cell culture

GSureTM Simplex kits for plant GSureTM Plasmid mini kit Trizin GlyseB

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DNA BIOLOGY PPT_Application.pdf · GPQ 05 G-Quant Bradford plus compatible protein assay Reagent...

Documents

Transcript of DNA BIOLOGY PPT_Application.pdf · GPQ 05 G-Quant Bradford plus compatible protein assay Reagent...