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    Diagnostic Tests

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    Overview of noninvasive and invasive

    diagnostic testing

    Diagnostic tests are either noninvasive orinvasive.

    Noninvasive means the body is not entered

    with any type of instrument. The skin and other

    body tissues, organs, and cavities remain intact.

    Invasivemeans accessing the bodys tissue,

    organ, or cavity through some type ofinstrumentation procedure.

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    Preparing a Client for Diagnostic

    Testing

    Diagnostic testing is a critical element of

    assessment.

    The nurse plays a key role in scheduling and

    preparing the client for diagnostic testing. The emphasis of pretest is on appropriate test

    selection, proper patient preparation,

    individualized patient education, and emotional

    support (Fischbach & Dunning, 2008, p. 9).

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    Preparing a Client for Diagnostic

    Testing

    Other key nursing measures to ensure client safety

    are to establish baseline vital signs, identify known

    allergies, and assess the effectiveness of teaching.

    The nurse has additional responsibilities:

    Preparing the room (e.g., adequate lighting)

    Gathering and charging for supplies used during the

    procedure Testing the equipment to ensure it is functional and safe

    Securing proper containers for specimen collection

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    Care of the Client during Diagnostic

    Testing

    Although the care of the client needs to beindividualized for a specific procedure.

    Standard Precautions are initiated when

    exposure to body fluids presents a threat to the

    safety of the caregiver.

    Protective barriers, such as gloves and a gown,

    should be used during invasive procedures.

    The nurse is responsible for labeling anyspecimen with the clients name, room number

    (hospitalized clients), date, time, and source of

    the specimen.

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    Care of the Client after Diagnostic

    Testing

    Nursing care post-procedure is directed towardrestoring the clients pre-diagnostic level of

    functioning.

    The client is monitored closely for signs ofrespiratory distress and bleeding.

    Some diagnostic procedures require that the

    vital signs be measured every 15 minutes for the

    first hour, then gradually decreased in frequencyuntil the client is stable

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    LABORATORY TESTS

    Common laboratory studies are usually simple

    measurements to determine how much or how

    many analytes (a substance dissolved in a

    solution, also called a solute) are present in a

    specimen.

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    Purpose of Lab Tests

    Laboratory tests are ordered by practioner to:

    Detect and quantify the risk of future disease

    Establish and exclude diagnoses

    Assess the severity of the disease process anddetermine the prognosis

    Guide the selection of interventions

    Monitor the progress of the disorder

    Monitor the effectiveness of the treatment

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    SPECIMEN COLLECTION

    The scheduling and sequencing of laboratory

    tests is an important function of the nurse. All

    tests requiring Venipuncture.

    Venipuncture, the puncturing of a vein with a

    needle to aspirate blood. Venipuncture is aninvasive procedure can be performed by various

    members of the health care team.

    Laboratories employ a phlebotomist, an

    individual who performs venipuncture, to collect

    blood specimens.

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    Arterial Puncture

    Arterial blood samples are drawn from a

    peripheral artery (e.g., radial or femoral) or froman arterial line.

    The arterial blood sample is collected in a 5-mL

    heparinized syringe. The syringe is then rotatedto mix the blood with the heparin to prevent

    clotting.

    Assessment of arterial blood gases (ABG)

    reveals the ability of the lungs to exchangegases by measuring the partial pressures of

    oxygen (PO2) and carbon dioxide (PCO2) and

    evaluates the pH of arterial blood.

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    Urine Collection

    Urine can be collected for various studies. The

    type of testing determines the method of

    collection.

    The different methods of urine collection are:

    Random collection (Routine analysis) Timed collection

    Collection from a closed urinary drainage system

    Clean-voided specimen

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    Urine Collection container

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    RANDOM COLLECTION

    The prescribing practitioner usually writes theorder for a UA (routine urine analysis), which is

    also called a random collection. It can be

    collected at any time using a clean cup. The

    urine does not have to be collected in a sterile

    container.

    TIMED COLLECTION

    Timed collection is done over a 24-hour period.The urine is collected in a plastic gallon

    container that contains preservative

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    COLLECTION FROM A CLOSED DRAINAGE

    SYSTEMA sterile specimen can be collectedfrom a client with an indwelling Foley catheter

    with a closed drainage system. A sterile

    specimen is used to culture the urine. The urine

    specimen should not be obtained from the

    drainage bag.

    CLEAN-VOIDED SPECIMEN Clean-voided

    (clean-catch, or midstream) specimen collectionis done to secure a specimen uncontaminated

    by skin flora. A clean-voided specimen should

    be obtained on first voiding in the morning.

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    Stool Collection

    Explain to the client why the stool specimen isbeing collected.

    Instruct the client to defecate into a clean

    bedpan or container, discarding tissue into the

    toilet.

    To determine the presence

    of occult (hidden) blood. To detect the presence of

    ova and parasites.

    To detect the presence of

    bacteria or viruses.

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    Sputum Specimens

    Sputum is the mucous secretion from the lungs,

    bronchi, and trachea. It is important todifferentiate it from saliva.

    Sputum specimens are usually collected for one

    or more of the following reasons: For C & S to identify a specific microorganism and its

    drug sensitivities.

    For cytology to identify the origin, structure, function,

    and pathology of cells. For acid-fast bacillus (AFB), which also requires serial

    collection, often for 3 consecutive days.

    To assess the effectiveness of therapy

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    Culture and Sensitivity Tests

    Culture refers to the growing of microorganisms

    to identify the pathogen.

    Culture and sensitivity (C&S) tests are

    performed to identify both the nature of the

    invading organisms and their susceptibility tocommonly used antibiotics.

    Blood Culture

    Swab (Throat) Culture

    Sputum Culture

    Urine Culture

    Stool Culture

    Wound Culture

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