Developing a compact and low-cost long-term live-cell ... · aim •make use of 3d printing, the...
Transcript of Developing a compact and low-cost long-term live-cell ... · aim •make use of 3d printing, the...
DEVELOPING A COMPACT AND LOW-COST LONG-TERM LIVE-CELL
IMAGING PLATFORM FOR BIOMEDICAL RESEARCH AND
EDUCATION PURPOSE (SCBE06)
CHUA YA XUAN
MENTOR: DR PUI TZE SIAN
INTRODUCTION
• LIVING THINGS ARE MADE UP OF CELLS AND CELLS ARE MADE UP OF
THOUSANDS OF MOLECULES THAT INTERACT WITH EACH OTHER.
• IN ORDER TO MAKE NEW DISCOVERIES ABOUT THE CELLULAR DYNAMICS AND
DEVELOP DRUGS TO COMBAT DISEASES THAT ARISE FROM DYSFUNCTIONAL
CELLS, SCIENTISTS ARE REQUIRED TO STUDY THE CONSTANTLY CHANGING
PATTERNS OF THESE MOLECULES
CURRENT METHODS
• LONGITUDINAL STUDIES
• TIME LAPSE MICROSCOPY
LIMITATIONS
COSTLYCELLS ARE NOT IN
OPTIMUM CONDITION BULKY
AIM
• MAKE USE OF 3D PRINTING, THE PROTOTYPING TECHNOLOGY, TO COME UP
WITH A COMPACT, LOW-COST AND EASY-TO-OPERATE LIVE-CELL IMAGING
PLATFORM FOR BIOMEDICAL RESEARCH AND EDUCATION PURPOSE
ENGINEERING GOALS
PHYSICAL FUCTIONAL
HUMIDITYTEMPERATURELOW-COSTCOMPACT
METHODOLOGY-HOUSING-
3D MODEL OF THE TOP VIEW OF INCUBATOR 3D MODEL OF THE SIDE VIEW OF INCUBATOR
• CAD software
• Acrylic glass
Holder for
CO2 sensor
Temperature &
humidity sensor
METHODOLOGY-MICROCONTROLLER-
• Arduino open-source Integrated
Development Environment
• C++ coding language
METHODOLOGY-INCUBATOR SYSTEM-
Top-view of the entire physical set-up Close-up view of incubator
Close-up view of LED panel
RESULTS-TEMPERATURE-
Graph of temperature against time to test how long the
incubator takes to reach the ideal temperature of 28 ˚C
• From a temperature of 24.4˚C, it took
85 minutes for the temperature to rise
to 28˚C, the optimum temperature we
aim to sustain throughout the
experiment.
RESULTS-TEMPERATURE-
Graph of temperature against time to test the stability of
the incubator in maintaining the optimum temperature
• Temperature only increases by 0.5˚C
in the next hour from 28˚C.
RESULTS-TEMPERATURE-
𝑻 =𝟏
𝑷 + 𝑩 + 𝑷𝟑 × 𝑪 + 𝑨− 𝟐𝟕𝟑. 𝟏𝟓
STEINHART-HART EQUATION
𝑃 = ln𝑅𝑝𝑎𝑖𝑟𝑒𝑑 × 𝑉𝑠𝑢𝑝𝑝𝑙𝑦
𝑉𝑜𝑢𝑡− 𝑅𝑝𝑎𝑖𝑟𝑒𝑑
T: Temperature in degree Celsius
A,B,C are Steinhart-Hart coefficients
A: 1.129148 × 10-3
B: 2.34125 × 10-4
C: 8.76741 × 10-8
• Software-based feedback loop using
the mean temperature
• Used to sustain temperature at 28 ˚C
RESULTS-HUMIDITY-
Graph of humidity against time to test the ability of the
incubator in maintaining an optimal humidity range
• 2 wells with 10ml of water each were
used during the experiment
• Humidity level of 85%-95% is ideal
• At the optimum temperature of 28 ˚C,
humidity increases from 86.8% to
90.3%.
DESIGN SPECIFICATIONS
Incubator
designed
Incubators on
the market
(average)
Size 17.5 cm x
12 cm x 7 cm
0.654g
28 cm x
20 cm x 9 cm
1.5 kg
Cost <$120 $15000-$20000
with microscope
CONCLUSION
PHYSICAL FUCTIONAL
TEMPERATURELOW-COSTCOMPACT HUMIDITY
ENGINEERING GOALS
FUTURE IMPROVEMENTS
HEATING ELEMENT VENTILATOR