Decisions Transcribe gene Splice RNA Destroy protein

download Decisions Transcribe gene Splice RNA Destroy protein

of 35

  • date post

    18-Jan-2016
  • Category

    Documents

  • view

    214
  • download

    0

Embed Size (px)

Transcript of Decisions Transcribe gene Splice RNA Destroy protein

  • Decisions Transcribe gene

    Splice RNADestroy protein

  • ..Or for the whole cell?

  • Think small, talk big.

    Sydney Brenner

  • Cooperative BindinglacZ

  • Regulated Recruitment

  • StepwiseEvolvability

  • 1.3

  • 1.3

  • 1.3

  • Synergy is easyEvolvability

  • New genesEvolvability

  • Specificity depends onconcentration or sequestrationThe Price

  • 1.16

  • 1.16

  • SH2 (P-Tyr)

    PTB (P-Tyr)

    SH3 (Pro)

    FHA (P-Thr)

    PH (PIP3)HTH

    HLH

    Zn Finger

    Winged Helix

    Homeodomain

    Recruiting Domains

  • Here are three ways to regulate presence of proteins in cellsthere are others THIS SHOULD (AND I THINK DOES) GO IN 3 STEPS..BUT FOR SOME REASON IT HALTS FOR A WHILE BETWEEN STEPSSOME FORMATTING PROBLEM? MAYBE REDRAW, TOP LINE, THEN ADD SECOND LINE, THEN THIRD..CAN YOU MAKE A BLACK BKGRD? THIS SHOULD (AND I THINK DOES) GO IN 3 STEPS..BUT FOR SOME REASON IT HALTS FOR A WHILE BETWEEN STEPSSOME FORMATTING PROBLEM? MAYBE REDRAW, TOP LINE, THEN ADD SECOND LINE, THEN THIRD..CAN YOU MAKE A BLACK BKGRD? THIS SHOULD (AND I THINK DOES) GO IN 3 STEPS..BUT FOR SOME REASON IT HALTS FOR A WHILE BETWEEN STEPSSOME FORMATTING PROBLEM? MAYBE REDRAW, TOP LINE, THEN ADD SECOND LINE, THEN THIRD..CAN YOU MAKE A BLACK BKGRD? TAKE OFF TITLE (MODE OF DNA BDG..) AND REPLACE WITH : COOPERATIVE BINDING.LABEL CAP SITE ON DNA AND MARK THE SITE WITH A BIT OF SHADING OR COLOR; AND LABEL PROMOTER AND MARK IT WITH A BIT OF SHADING OR COLOR TAKE OFF THE TWO GIZMOS ON CAP SO IT IS A SIMPLE SPHERE TAKE OFF THE TITLE AND TEXTCAN YOU SEPARATE A BIT THE MEDIATOR FROM THE POLYMERASE..I THINK TFIIH SHOULD ALSO BE A BIT SEPARATED..LABEL MEDIATORBE SURE IT HAS GAL11 IN ITSEE P 120, FIG 3.2 OF G AND S FOR A BETTER PICTURE OF MEDIATOR..THERE IS A RECENT REVIEW IN CELL OR MOLECULAR CELL CALLED MEDIATOR, A CONSERVED STRUCTURE OR SOMETHING LIKE THAT THAT MIGHT GIVE AN EVEN BETTER PICTURE...MAKE SAGA BLUE ALSOMAKE ACTIVATOR (THE GREEN THING) LOOK LIKE BALL (ON DNA) AND SQUIGGLEY THING REPRESDENTING ACTIVATING REGION AS IN PREVIOUS SLIDE..

    The Real Deal: instead of neat dedicatedd machines for each substrate (eg gene) have for each case one E and large array of possible substrates and zilliuons of locators, inhibitors, etcregulation as subsrarte choice, determijned by recruitmentdetermijned This slide is finejust to show what previous slide should look like..As go to higher euks must integrate more signals such that have more intricate patterns of expressionAdd energiescan touch anywhere Integration of three signals.. Structure of enhancesome not criticalreiterated single guys work pretty well..if strengthen promoter need only 2 of the three (which I guess means they bind coop first..)cf Thanos..nb: each activator works together in combinations with different activators: combinatorial controlmeans that # of regulators is much smaller than total number of programs (I think) because have combinationsSopecificity determined not by active sites alone but by locators plus active site..Follows from principles of coop bdg Huge huff and puff depends on conc of Rs not getting too highso have autoregulatory negative mechanism (as with lac case, initial thing works, but as add features gets better and better)Domains for P-D, P-P, P-Rall serve same fnctnkey concept.. Change the order so that the bottom thing (the promoter) is on the topthen the text reads:

    A promter (they see the promoter) then click looks like a receptor and they see the picture of the receptor..