COURSE OUTLINE

ERT 317Kejuruteraan Biokimia / Biochemical Engineering

Semester 12015/2016

Nilai unit/ Number of unit: 4Jenis kursus/ Course type: Teras / CorePrasyarat/ Prerequisite: ERT 106 Biochemistry

Sinopsis kursus / Course synopsis:  This course focuses on the importance of bioprocessengineering elements such as enzyme technology, fermentation and bioconversion technology. Mathematical representations of enzymatic reaction as well as microbial systems are featured among lecture topics especially on kinetics study.

Hasil Pembelajaran / Learning Outcomes: At the end of the course, students are expected to be: Ability to apply knowledge and solve engineering problem

related in developing enzyme reactions based on its kinetics study and applied catalysis.

Ability to identify, formulate, analyze and design solutions complex engineering problems using mathematics principles, natural sciences and engineering sciences for batch and continuous microbial systems, metabolic pathways and kinetics study.

Ability to propose bioconversion technologies of microbial, plant and animal cell culture for the production of value-added product and defend it through verbal presentation in a group.

Senarai eksperimen / List of experiments: • Lab 1: To analyze the effect of enzyme and substrate

concentration on enzyme kinetics study

• Lab 2: To analyze the effect of immobilization method on the retention of enzymatic activity

• Lab 3: To analyze the effect of environmental conditions on growth kinetics of S. cerevisae in batch process

• Lab 4: Effect of cell immobilization on dye removal

by bioabsorbent • Lab 5: To organize an open-ended experiment based

on bioconversion technology approaches

Pendekatan pembelajaran / Learning approach:

 •(i) Lecture = 42 hours •(ii) Laboratory = 10 hours •(iii) Tutorial = 12 hours •(iv) PBL = 9 hours

Sumbangan penilaian / Evaluation contribution:  Peperiksaan/ Examination: 70 % • Mid Term Examinations = 20 %• Final Examination = 50 % Penilaian Berterusan/ Continual Assessment: 30

%

• Quizzes/Assignment =10 %• Lab report =10 %• Integrated Project =10 %

Senarai buku teks dan rujukan / List of text books and references :  • Shuler, M. L. and Kargi, F. (2014). Bioprocess Engineering: Basic

Concept. 2nd Ed. Upper Saddle River, NJ: Prentice Hall PTR

• Rao, D.G. (2010). Introduction to Biochemical engineering. 2nd Ed. Tata McGraw Hill Education Private Limited

• Dutta, R. (2008). Fundamentals of Biochemical Engineering. New Delhi: Ane Books India

• Katoh, S. and Yoshida, F. (2009). Biochemical Engineering: A Textbook for Engineers Chemists and Biologists. Weinheim: Wiley-VCH Verlag GmbH & Co

• Nielsen, J., Villadsen, J. and Gunner L. (2011). Bioreaction Engineering Principles. 3rd Ed. New York: Springer Science+Bussiness Media

Minggu/WeekKandungan Kursus / Course Contents

(Panduan/Guidelines)

Tugasan Bacaan/Reading

Assignment

Pensyarah/Lecturer

Week 1 - 2(7/09 – 18/09)

CHAPTER 1: ENZYME KINETICS AND APPLICATIONS

Chapter 1.1: Kinetics of Enzyme Catalyzed Reactions

Introduction - Classification of enzymes according to the reaction catalyzed and how enzymes work. Enzyme kinetics - Mechanistic model for simple enzyme kinetics, determination of rate parameters for Michaelis-Menten kinetics; models for more complex enzyme kinetics; effects of pH and temperature and insoluble substrates

Chapter 1 Mrs. Hafiza Shukor

Week 3(21/09 – 25/09)

CHAPTER 1: ENZYME KINETICS AND APPLICATIONS

Chapter 1.2 : Applied Enzyme Catalysis

Immobilized enzyme systems - Methods of immobilization, diffusional limitations in immobilized enzyme systems, electrostatic and steric effects in immobilized enzyme systemsEnzyme production and application – Large scale production of enzymes, medical and industrial utilization of enzymes

Chapter 1

Mrs. Hafiza Shukor

Week 4 - 5(28/9 – 9/10)

CHAPTER 2: MAJOR METABOLICS PATHWAY

Introduction to metabolism and bioenergetics. Glucose metabolism: Glycolysis and TCA cycle, respiration. Metabolism of nitrogenous compounds and hydrocarbons. Overview of biosynthesis for small and macromolecules, anaerobic and autotrophic metabolism.

Chapter 2

Mrs. Hafiza Shukor

Week 6 - 7(12/10 – 23/10)

CHAPTER 3 : KINETIC OF GROWTH IN BATCH AND CONTINOUS CULTURES

Chapter 3.1 : Kinetic of Growth in Batch CultureQuantifying cell concentrations, growth patterns and kinetics in batch culture, effects of environmental conditions on growth kinetics, heat generation by microbial growth.

Chapter 3 Dr. Saleha

RANCANGAN MENGAJAR/LESSON PLAN

Week 8-9 (26/10 – 6/11)

CHAPTER 3 : KINETIC OF GROWTH IN BATCH AND CONTINOUS CULTURES

Chapter 3.2 : Kinetic of Growth in Continuous Culture

Some specific devices for continuous culture, derivation for ideal chemostat, application of chemostat and its deviation of ideality.

Chapter 3 Dr. Saleha

Week 10(9/11 – 13/11)

Cuti Pertengahan Semester / Mid-semester Break

Week 11 (16/11 – 20/11)

CHAPTER 4: STOICHIOMETRY OF MICROBIAL GROWTH AND PRODUCT FORMATION Overall growth stoichiometry and design medium formulation, stoichiometric calculation of elemental balances and degree of reduction, theoretical predictions of yield coefficients.

Chapter 4 Dr. Saleha

Week 12-13 (23/11 – 4/12)

CHAPTER 5 : BIOPROCESS CONSIDERATIONS IN USING ANIMAL AND PLANT CELL CULTURES

Chapter 5.1 : Considerations in Using Animal Cell CultureStructure and biochemistry of animal cells, methods used for the cultivation of animal cells, products of animal cell cultures

(3 hours)

Chapter 5.2 : Considerations in Using Plant Cell CultureThe importance and advantages of plant cell culture, plant cells in culture compare to microbes, methods used for the cultivation of plant cells, economics of plant cell tissue culture

Chapter 5 Dr. Saleha

Week 14 - 15(7/12 – 18/12)

CHAPTER 6: BIOCONVERSION TECHNOLOGIES FOR INDUSTRIAL BIOPROCESSES

Type of bioconversion approaches e.g solid-state and liquid-state, advantages and disadvantages of bioconversion, important factors for bioconversion. Application of microbial, plant and animal cell culture in bioconversion technologies utilizing waste as substrate for production of value-added product.

Chapter 6 Dr. Saleha

Week 16(21/12 – 25/12)

Minggu Ulangkaji / Revision Weeks

Week 17 - 20(28/12 – 15/01)

Peperiksaan Akhir Semester/ Final Examination

 RK 28 - Year 3

8.00-9.00 9.00-10.00 10.00-11.00 11.00-12.00 12.00-1.00 1.00-2.00 2.00-3.00 3.00-4.00 4.00-5.00 5.00-6.00 6.00-7.00

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